ライフサイエンスコーパス: directed mutation

1 combination of cloning, complementation and directed mutation.

2 latory and catalytic phenomena by studies in directed mutation.

3 ymes where key residues were altered by site-directed mutation.

4 in DNA translocation activity caused by site-directed mutations.

5 of Asp-40 of VCAM-1 through the use of site-directed mutations.

6 was investigated through a series of target-directed mutations.

7 e (PhoA) fusions, precise deletions and site-directed mutations.

8 null allele and Trbl transgenes bearing site-directed mutations.

9 ly control gene expression or to induce site-directed mutations.

10 pressing A beta class II molecules with site-directed mutations.

11 ent viruses were confirmed by analyzing site-directed mutations.

12 amples, and (iii) env clones containing site-directed mutations.

13 Additional site-directed mutations affecting this interval commonly impa

14 e results underscore the difficulty of using directed mutations alone as a means of mapping the activ

15 hat affects dimerization as shown by limited directed mutations along the transmembrane domains.

16 Site-directed mutations also revealed the electronic quenchin

17 In vivo deletion and site-directed mutation analyses confirm that one GATA element

18 n sites into two different peptides and site-directed mutation analyses of acylation sites, often ser

19 n sites into two different peptides and site directed mutation analyses of acylation sites.

20 Sequential deletion and site-directed mutation analyses of the VEGF promoter demonstr

21 promoter, gel mobility shift assays and site-directed mutation analyses suggest that the putative NF-

22 re, we present a series of deletion and site-directed mutation analyses to identify amino acids in RI

23 Site-directed mutation analysis identified Arg(205), which is

24 Sequential deletion and site-directed mutation analysis of the CD1 promoter have iden

25 Using site-directed mutation and with femtosecond resolution, we ex

26 Using an infectious clone, site-directed mutations and 3' untranslated region (3'UTR) ex

27 rved residues, we generated a series of site-directed mutations and analyzed their impact on AL1 func

28 spontaneous mutations as well as constructed directed mutations and deletions in ppsR.

29 UNX site (+526 to +508), as revealed by site-directed mutations and exogenous expression of RUNX fact

30 n and related ansamycins, a series of target-directed mutations and heterologous gene expressions wer

31 Here, we used site-directed mutations and molecular dynamic simulations to

32 Through a series of site-directed mutations and phage display selections, the seq

33 Site-directed mutations and small internal deletions made in

34 ates of random mutation, the specific stress-directed mutations are also enhanced.

35 of many nucleic acid enzymes, the first site-directed mutations are made at conserved acidic active r

36 A site-directed mutation at Lys-1016 significantly decreased WR

37 Site-directed mutations at Arg(662), Pro(667), and Pro(670) w

38 xygen of biotin has been conducted with site-directed mutations at Asn 23, Ser 27, and Tyr 43.

39 In PS II, the site-directed mutations at D1 His198 affect the P(+)--P-absor

40 il arrangement was disfavored by adding site-directed mutations at F241 and F243 in the CH2 domains,

41 previously reported making a number of site-directed mutations at five sites located in the cofactor

42 intermembrane loops and of a series of site-directed mutations at hamster MFT residues highly conser

43 Site-directed mutations at multiple positions along the RNA-b

44 unction of Ctr1 containing deletions or site-directed mutations at the C terminus indicates a structu

45 The site-directed mutations at the conserved amino acid residues

46 We constructed a series of site-directed mutations at the dimer interface, and tested th

47 By making site-directed mutations at these positions and analysing the

48 Site-directed mutations at these residues reduce the rate of

49 We have made site-directed mutations at these residues, measured thermodyn

50 g loss-of-function mutations of EF3 and site-directed mutations based on the homology of the edema fa

51 A structure-guided, site-directed mutation-based inquiry of the catalytic contrib

52 Site-directed mutations betaE197C, betaE197D, and betaE197S i

53 ionally, this approach enables not only site-directed mutations, but also the insertion of DNA strand

54 ricus strain 98/2, a popular strain for site-directed mutation by homologous recombination.

55 To test this possibility, site-directed mutations by alanine substitution were introduc

56 instability in Friedreich's Ataxia is a DNA-directed mutation caused by improper DNA structure at th

57 id-targeted RET/PTC1 mutants carrying a site-directed mutation changing tyrosine (Y) to phenylalanine

58 ylate a recombinant Rpg1 inactivated by site-directed mutation confirmed that Rpg1 autophosphorylatio

59 Assays and directed mutations confirmed this activity and showed th

60 scopic measurements in combination with site-directed mutations constitute one important source of in

61 Site-directed mutation constructs were expressed in Xenopus o

62 of the mathematical issues arising from the directed mutation controversy, thereby offering alternat

63 Structure-guided site-directed mutations converted SLAC1 into a SLAH2-like nit

64 Site-directed mutations D262C, D262H, D262N, and D262T were m

65 In conclusion, a site-directed mutation designed to improve the stability of p

66 obacter sphaeroides are investigated by site-directed mutations designed to alter the electrostatic e

67 Introduction of site-directed mutations designed to disrupt the hydrophobic f

68 Some site-directed mutations did not exhibit suppressor activity a

69 nt with previous reports, we found that site-directed mutations disrupting the protein-carbohydrate i

70 Kcv-like channels will be used to guide site-directed mutations, either singularly or in combination,

71 ion of recombinant viruses containing a site-directed mutation, encoding an amino acid substitution,

72 The results of site-directed mutation experiments established that icIL-1RaI

73 Isl-1 in overexpression, knockdown, and site-directed mutation experiments.

74 Furthermore, analysis of site-directed mutations found two putative Mg(2+)-binding res

75 The site-directed mutations H82A and Q222A (residues near the hem

76 In this study, site-directed mutations have been introduced to the human CSB

77 lar, we explored the effects that these site-directed mutations have over the enzyme kinetics with va

78 of new evidence that supposedly supports the directed mutation hypothesis has accumulated.

79 Within the latter region, site-directed mutations identified a consensus sequence "acac

80 These site-directed mutations identified a novel negatively charged

81 establish an example of transposon-mediated directed mutation, identify the protein responsible and

82 A site-directed mutation in a putative gene that resides downst

83 cted COH1-GT2, a strain with a nonpolar site-directed mutation in glnQ.

84 f CS-B-null cells and mutant cells with site-directed mutation in the motif VI of the putative helica

85 but not with an orf4 gene containing a site-directed mutation in the putative zinc metalloprotease m

86 ze the peptidyl transferase activity of site-directed mutations in 23S rRNA at positions G2252, U2506

87 (EAL) from Salmonella typhimurium with site-directed mutations in a conserved arginine residue (R160

88 activation of ARD1 activity was tested using directed mutations in a loop near the substrate-binding

89 Site-directed mutations in BadR and the presence of phosphory

90 Using a series of site-directed mutations in both the genomic and cDNA context,

91 In this study, we generated a series of site-directed mutations in conserved sequence and structural

92 These are the first site-directed mutations in CP 43 to show such an effect.

93 Naturally occurring variants and directed mutations in DaaE have been generated and analy

94 Site-directed mutations in GIV's GEF motif disrupt its abilit

95 Site-directed mutations in irp6A, a gene previously reported

96 Site-directed mutations in its putative dimerization interfac

97 is approach promises to permit generation of directed mutations in many types of cells and organisms.

98 f1 interaction surface on Rpo41, making site-directed mutations in Rpo41 at positions predicted to re

99 Using site-directed mutations in SP-A, we determined that both the

100 Moreover, site-directed mutations in specific Ikaros-binding sites redu

101 l cis-acting elements were selected for site-directed mutations in the activation domain.

102 dependent functions of VP1, we created site directed mutations in the B3 domain that disrupted DNA b

103 is diminished in constructs containing site-directed mutations in the Cbfa1 site indicate that Cbfa1

104 Site-directed mutations in the conserved residues of the chro

105 f a defined series of p9 truncation and site-directed mutations in the context of a reference infecti

106 Analysis of site-directed mutations in the CSF-1R further suggests that a

107 Site-directed mutations in the cytoplasmic domains of betaPS

108 multiple bands, known as A states, and site-directed mutations in the distal pocket of myoglobin.

109 Furthermore, we demonstrate that site-directed mutations in the esc protein previously shown t

110 Site-directed mutations in the Escherichia coli ssb gene were

111 Spontaneous or directed mutations in the gene that encodes citrate synt

112 Homologous recombination can produce directed mutations in the genomes of a number of model o

113 Site-directed mutations in the IBS indicate a probable cis-ac

114 Site-directed mutations in the most highly conserved region o

115 We then created a series of site-directed mutations in the OccR-binding site.

116 tosystem I (PS I) is investigated using site-directed mutations in the phylloquinone (QK) and FX bind

117 Site-directed mutations in the pore-lining M2 helix allowed t

118 characterized VP6 activity by creating site-directed mutations in the putative functional helicase d

119 n contrast, the debilitating effects of site-directed mutations in the single nuclear localization si

120 We analyzed the effects of site-directed mutations in the SUC2 promoter of Saccharomyces

121 r-reporter genes with 5'-truncations or site-directed mutations in the TGA elements showed that both

122 Several site-directed mutations in the transposase abolished activity

123 erized the effects of combinatorial and site-directed mutations in the two beta-turns of peptostrepto

124 basis of this association, we generated site-directed mutations in the various domains of CD82 and us

125 competence-based method for generating site-directed mutations in the wild-type fimbriated A. actino

126 tes in U3 sequences of MCF 247 and that site-directed mutations in these sites greatly diminish this

127 Site-directed mutations in this pocket impaired intracellular

128 expressing Flag-tagged CCR2B containing site-directed mutations in this region, 21-26, including a co

129 863-nt region and serial deletions and site-directed mutations indicated that the most distal PPRE (

130 n the enzyme active site as a result of site-directed mutations, inhibitor binding, and changes in pH

131 l ATPase, we have introduced equivalent site-directed mutations into the ATPbinding motif of each RPT

132 Directed mutation is a proposed process that allows muta

133 etermined by escape mutant analysis and site-directed mutation, is located in a non-aqueous island.

134 Functional analyses of site-directed mutations (K68D, K68E, K68N, K68P, and K68R) in

135 Site-directed mutations made in ori and Asn10 of Rep protein

136 Here we describe a novel mechanism of directed mutation mediated by the transposon, IS5 in Esc

137 The site-directed mutations modify the protein environment of the

138 the "helical cap" by evaluating single site-directed mutations, namely, L29A, I32E, L35A, L35E, L35R

139 Therefore, we conducted site-directed mutation of 15 amino acid residues of NuoM and

140 Deletion of the kinase domain or site-directed mutation of a subset of known PKCtheta phosphor

141 Site-directed mutation of AKT at T430 and T479 decreased O-Gl

142 Site-directed mutation of amadoriase II (R112E, R114E) result

143 Site-directed mutation of each of these conserved residues in

144 Deletion or site-directed mutation of either or both GATA sequences resul

145 Site-directed mutation of Glu92 to glutamine had a detrimenta

146 Site-directed mutation of His-309 to Asn and Asp-210 to Ala r

147 Site-directed mutation of His30 to alanine destroyed its phos

148 Site-directed mutation of individual nucleotides within these

149 Moreover, site-directed mutation of Leu-219, the conserved leucine resi

150 eventing the FAM83B/EGFR interaction by site-directed mutation of lysine 230 of FAM83B suppressed PLD

151 Site-directed mutation of lysine to glutamate at position 72

152 Directed mutation of P1 was used to locate regions that

153 Importantly, the site-directed mutation of raptor at one phosphorylation site,

154 However, site-directed mutation of sequences located between -35 and -

155 confer an aggregation phenotype, (iii) site-directed mutation of specific residues can abolish recep

156 Site-directed mutation of STAT binding sites on the COX-2 pro

157 Site-directed mutation of T91 to alanine abolished PKA-mediat

158 Site-directed mutation of the -533-, -759-, and -773-bp GREs

159 Site-directed mutation of the -564- to -558-bp sequence resul

160 armacologically, by leptomycin B, or by site-directed mutation of the ALX NES, impairs CD28-mediated

161 ther species, this study introduced the site-directed mutation of the ATPase motif II in the human CS

162 Site-directed mutation of the CRE-II element caused a marked

163 Directed mutation of these residues supported this regio

164 ket, and we had previously found that a site-directed mutation of this amino acid resulted in a loss

165 Site-directed mutation of this AP-1 binding site eliminated t

166 Site-directed mutation of this HRE substantially reduced the

167 47 to catalysis is investigated here by site-directed mutation of this residue to His (Y447H), the fi

168 Single site-directed mutation of this TonE site led to a 54 +/- 5% (

169 Site-directed mutation of three consensus PKA phosphorylation

170 Site-directed mutation of two putative NFAT binding sites bet

171 Analysis of nine site-directed mutations of -246/-99 region reveals that both

172 Alternative substrates and site-directed mutations of active-site residues are used to p

173 Site-directed mutations of both OCT-1 and CAAT motifs abrogat

174 Site-directed mutations of both OCT-1 and CAAT motifs substan

175 Site-directed mutations of conserved residues in the first RR

176 The effects of several other single site-directed mutations of conserved residues of HlyC on diff

177 The effects of single site-directed mutations of conserved residues of HlyC on diff

178 coli MG1655 to its derivatives carrying site-directed mutations of genes for enterobactin synthesis (

179 Specific site-directed mutations of individual N terminus residues wer

180 Site-directed mutations of key residues within the hydrophobi

181 Site-directed mutations of neighboring conserved P1 residues

182 a series of other tumor-associated, and site directed mutations of NFKB2.

183 Additionally, site-directed mutations of particular amino acid residues in

184 n heat capacity by means of a series of site-directed mutations of PwTBP.

185 We show that directed mutations of residues at a particular corner of

186 We explore the use of site-directed mutations of scytalone dehydratase to study inh

187 Site-directed mutations of serine-303 and threonine-494, pote

188 deletions of halpha(1C)1b promoter and site-directed mutations of the 2 CREB binding cis-elements in

189 Site-directed mutations of the conserved C93, C109, C116 and

190 Site-directed mutations of the human insulin promoter mapped

191 Thus, site-directed mutations of the pocket and lid loop led to sig

192 Using site-directed mutations of the redox-active tyrosines at the

193 Site-directed mutations of the residues involved in recogniti

194 Through the combined strategies of site-directed mutations of these amino acid residues and cati

195 Site-directed mutations of this region did not affect TCL1 se

196 With the two rat isoforms, site-directed mutations of two locations within the lipid bin

197 Functional inactivation by site-directed mutations of two Smad binding sites on the RON

198 s new evidence of a secondary effect of site-directed mutation on a RC chromophore.

199 studied to investigate the effects of a site-directed mutation on bound substrate conformations.

200 f posttranscriptional modifications and site-directed mutations on both the structure and function of

201 ied to investigate the effects of these site-directed mutations on bound-substrate conformations.

202 assessed with regard to the effects of site-directed mutations on class I and II activities.

203 clear import, we analyzed the effect of site-directed mutations on IE1 subcellular distribution.

204 pA promoter by measuring the effects of site-directed mutations on promoter activity.

205 The effects of the site-directed mutations on residues within the active site of

206 ere assessed by studying the effects of site-directed mutations on the binding affinity of (125)I-ASI

207 distribution that could be abrogated by site-directed mutations or treatment with the nuclear export

208 However, site-directed mutations predicted to impair ATP binding or hy

209 on with other studies in crystallography and directed mutation, puts the phosphate regulatory site at

210 Site-directed mutations R177A and R177K in the gene encoding

211 eptide synthetase cores, as well as the site-directed mutations, resulted in derivatives that retaine

212 Considerations of a large number of site-directed mutations reveal that two distinct regions, nam

213 Analysis of site-directed mutations revealed stereochemical and volume-de

214 An analysis of site directed mutations revealed that chaperone dimerization

215 ysis of transposase proteins containing site-directed mutations revealed the importance of the conser

216 the basis of these results, a number of site-directed mutations (SDM) were designed that were intende

217 The site-directed mutations studied here enable the individual is

218 ains of hmGlu2 and hmGlu3 combined with site-directed mutation studies has clarified the underlying m

219 ed by earlier X-ray crystallography and site-directed mutation studies.

220 ied in the screen as well as additional site-directed mutations suggest that the mode of DNA recognit

221 A series of domain swap and site-directed mutations, supported by structural comparisons,

222 between permease orthologs, a series of site-directed mutations targeted at residues within the two c

223 Site-directed mutations targeting the N-deacetylase catalytic

224 an active-site cysteine, we constructed site-directed mutations that altered each of the three cystei

225 In this study, we constructed four site-directed mutations that changed aspartate 205 to alanine

226 mants that express ESC proteins bearing site-directed mutations that disrupt ESC-E(Z) binding in vitr

227 Similarly, site-directed mutations that disrupt this site reduce promote

228 Additional site-directed mutations that disrupted ethylene binding in ye

229 Site-directed mutations that made the coupling sequences comp

230 mic caspase-3 cleavage with and without site-directed mutations that mimic phosphorylation at Thr231/

231 yll-protein interactions by a series of site-directed mutations that replaced residue Leu M160 with h

232 Removal of the Env aa from v-Rel or site-directed mutations that revert the three mutant residues

233 Site-directed mutations that strongly impair transport or tha

234 Using site-directed mutations that substitute minK and KCNE3 residu

235 Site-directed mutations that substitute Phe for Trp241 and Ty

236 Additional site-directed mutations that were predicted to further streng

237 beta, and, using chimeric analysis and site-directed mutations, that the central region of PP1c beta

238 n enzyme catalysis were investigated by site-directed mutation to alanine.

239 -159 was shown by deletion analysis and site-directed mutation to be critical for promoter activity.

240 of hexokinase I, specifically engineered by directed mutation to block dimerization, remains monomer

241 nt work, we constructed four individual site-directed mutations to change each of these two residues

242 emical denaturant, mechanical force and site-directed mutations to demonstrate the presence of multip

243 We then used site-directed mutations to identify directly repeated sequenc

244 In addition, we use site-directed mutations to identify the most important antibo

245 Site-directed mutations to individual cysteine residues in ea

246 proposed active site were subjected to site-directed mutations to investigate their possible functio

247 ical analysis of PLZF-BTB/POZ harboring site-directed mutations to probe structure-function relations

248 In this study, we used directed mutations to show that all six blades of the HC

249 The site-directed mutations, W352C, W352L, and W352A, exhibit an

250 en these paired nucleotides, one set of site-directed mutations was constructed at positions 690:697

251 ional activation of gltBDF, a series of site-directed mutations was generated.

252 engths from the visible to the UV and a site-directed mutation we unambiguously determined that the p

253 tive site, and based on the analysis of site-directed mutations we conclude that an active-site cyste

254 Using site-directed mutations we have examined the contributions of

255 bination with a series of deletions and site-directed mutations, we determined that the first 22 bp o

256 With various site-directed mutations, we identified all possible electron

257 f AAV2-AAV8 hybrids and the creation of site-directed mutations, we mapped the domain that directs th

258 om both hydrogen-deuterium exchange and site-directed mutations, we suggest structural differences be

259 Site-directed mutations were also created in specific surface

260 Site-directed mutations were also made at residues (Phe479 an

261 Site-directed mutations were constructed at Glu-23 to assess

262 Site-directed mutations were constructed to inactivate one or

263 h pyrimidine dimer-containing DNA, four site-directed mutations were created, the mutant enzymes expr

264 An extensive series of deletion and site-directed mutations were evaluated for their ability to r

265 ocyanin/cytochrome c6 and the PSI core, site-directed mutations were generated in the luminal J loop

266 Nine site-directed mutations were introduced at residue 117, using

267 In the studies presented here, site-directed mutations were introduced at two other conserve

268 Site-directed mutations were introduced in motif III S5 regio

269 n Qin-induced oncogenic transformation, site directed mutations were introduced in the winged helix (

270 Site-directed mutations were introduced into each of these RR

271 Site-directed mutations were introduced into HA-tagged Gag to

272 For this purpose, site-directed mutations were introduced into the full-length

273 incorporation and nuclear localization, site-directed mutations were introduced into the vpx gene of

274 Random and site-directed mutations were introduced into this segment of

275 Site-directed mutations were introduced to replace D1-His198

276 obic nature of this portion of p6(Gag), site-directed mutations were made at the minor protease cleav

277 ns between 23S rRNA and aminoacyl tRNA, site-directed mutations were made at the universally conserve

278 tions between Rubisco activase and ATP, site-directed mutations were made at two species-invariant as

279 In this study, site-directed mutations were made in the carboxyl-terminal nu

280 resected the full D-domain; therefore, site directed mutations were made to attribute precise sequen

281 Site-directed mutations were made to the phosphate-binding lo

282 Site-directed mutations were made to the phosphate-binding lo

283 to elucidate the function of subunit E, site-directed mutations were performed at the amino terminus

284 Site-directed mutations were prepared in a series of residues

285 Site-directed mutations were produced in the distal segments

286 ys (denoted pathway 1) are sensitive to site-directed mutation, whereas residues in the other propose

287 Here we observe that site-directed mutations which eliminate the dyad symmetry of

288 Moreover, a site-directed mutation within the PilZ domain of CelA, the ca

289 By using mutants with site-directed mutations within a predicted C-terminal process

290 s, performed with an extensive panel of site-directed mutations within each of the six kelch motifs,

291 We show that site-directed mutations within either the -10 or the -35 regi

292 ities), a series of 16 alleles carrying site-directed mutations within FAT1 were constructed and anal

293 Site-directed mutations within Ll.ltrB, either in the catalyt

294 insertionally inactivated construct or site-directed mutations within the active site, demonstrated

295 s of Swi6, we generated both random and site-directed mutations within the ankyrin repeat domain of S

296 We then created deletion and site-directed mutations within the conserved PI 3-kinase doma

297 this ectoenzyme, human CD39 was modified by directed mutations within these ACRs or by sequential de

298 Directed mutations within these so-called eIF4G homology

299 Ca(2+) concentrations demonstrate that site-directed mutations within this region interfere selectiv

300 Deletion and site-directed mutations within this region revealed that the