ライフサイエンスコーパス: discoidal

1 oE and prepare apoE containing reconstituted discoidal 1-palmitoyl-2-oleoyl-l-phosphatidylcholine (PO

2 and relative alignment of apoA-I monomers on discoidal (9.4 nm) reconstituted high density lipoprotei

3 rt the discovery of polymer nanodiscs, i.e., discoidal amphiphilic block copolymer membrane patches e

4 The r-HDL were found to be discoidal and in the size range of native HDL.

5 ular determinants for stabilization of model discoidal and plasma spherical HDL.

6 esidues 1-43 or 44-65 obviously discriminate discoidal and spherical reconstituted HDL particles desp

7 In both discoidal and spheroidal rHDL, DPPC containing r-HDL wer

8 ein (HDL), and the formation of spherical or discoidal apoE-containing HDL.

9 Discoidal apoE3-phospholipid complexes using a substitut

10 llipsoidal DSH particles rapidly collapse to discoidal bilayer structures.

11 combines with phospholipids to form similar discoidal bilayers and may prove to be superior to human

12 tate that rHDL particles are protein bounded discoidal bilayers.

13 ts indicated that each of the 2 molecules of discoidal bound apoA-I exists in multiple conformations

14 This result, which is not limited to model discoidal but also extends to plasma spherical HDL, help

15 The composition of the 100 A discoidal complex is approximately 5 [1-44]apoA-I and ap

16 all-atom molecular dynamics simulation on a discoidal complex made of 1-palmitoyl-2-oleoyl-sn-glycer

17 lexes formed under similar conditions: small discoidal complexes (approximately 3:1 weight ratio, app

18 ed at an initial 1:1 weight ratio and larger discoidal complexes (approximately 4.6:1 weight ratio, a

19 asma apoA-I to form two sizes of homogeneous discoidal complexes and thus may be responsible for apoA

20 Nascent HDL are discoidal complexes composed of a phospholipid bilayer s

21 All four discoidal complexes displayed similar abilities to remov

22 olution structural view of the peptide.lipid discoidal complexes formed by a class A amphipathic alph

23 s suggests that the kinetic stability of the discoidal complexes is dominated by the lipid-lipid rath

24 choline (DMPC) bilayer vesicles into smaller discoidal complexes is enhanced as a function of decreas

25 Thermal unfolding of discoidal complexes of apolipoprotein (apo) C-1 with dim

26 t ambient temperatures, protein oxidation in discoidal complexes promotes their remodeling into large

27 displayed lipid-binding abilities and formed discoidal complexes that were similar in major diameter

28 At higher ratios, discoidal complexes were shown to exist together with a

29 dimyristoylphosphatidylcholine vesicles into discoidal complexes with an efficiency similar to that o

30 At 1:1 DMPC:[1-44]apoA-I (w/w) ratio, discoidal complexes with composition approximately 4:1 (

31 es of dimyristoylphosphatidylcholine to form discoidal complexes with diameters in the range of 15-20

32 , the so-called globular domain of ApoA1, in discoidal complexes with phospholipids and increasing am

33 lphosphatidylcholine/pyrene-R61C/E255C/apoE4 discoidal complexes, pyrene excimer fluorescence emissio

34 e reconstituted into phospholipid-containing discoidal complexes.

35 ement of the deletion mutant proteins in the discoidal complexes.

36 econstituted high density lipoprotein (rHDL) discoidal complexes.

37 uffling and a failure to acquire the typical discoidal erythroid shape but they can enucleate.

38 Discoidal forms of high density lipoproteins (HDL) are c

39 ein (apo) A-I, in spheres vs. better studied discoidal forms.

40 ch undergo regulated exocytosis of subapical discoidal/fusiform vesicles (DFV) during bladder filling

41 The discoidal/fusiform vesicles (DFV) of bladder umbrella ce

42 n bladder umbrella cells a subapical pool of discoidal/fusiform-shaped vesicles (DFVs) undergoes Rab1

43 by use of a bolus injection of reconstituted discoidal HDL (recHDL).

44 itatively with loss of LCAT activity in both discoidal HDL and HDL(3), the enzyme's physiological sub

45 ts corroborate our earlier analysis of model discoidal HDL and indicate that a kinetic mechanism prov

46 ed two general models of apoA-I structure in discoidal HDL complexes.

47 consistent with the destabilization of model discoidal HDL observed upon increasing the A-II to A-I r

48 l three monomers of apo A-I are bound to the discoidal HDL particle in a hairpin conformation.

49 4-243) wrapped around the circumference of a discoidal HDL particle.

50 hree monomers of apo A-I to a 150 A diameter discoidal HDL particle.

51 ts of apoA-I were analyzed in reconstituted, discoidal HDL particles composed of phospholipids contai

52 We examined the effects of the size of discoidal HDL particles containing wild-type (WT) apoA-I

53 phobic residues in the C-terminus, generated discoidal HDL particles indicating a defect in their con

54 In this work, discoidal HDL particles of different size were reconstit

55 binding to cells and the compositions of the discoidal HDL particles that are produced.

56 rt the belief that apo A-I binds to lipid in discoidal HDL particles via the belt conformation.

57 po) A-I in large (9.6 nm) and small (7.8 nm) discoidal HDL particles were determined by hydrogen-deut

58 are discussed for the binding of apo A-I to discoidal HDL particles with diameters identical to thos

59 made of a membrane-PL mixture and FC yields discoidal HDL particles with diameters in the range 9-17

60 Molecular belt models for discoidal HDL particles with differing diameters are pre

61 rp264Ala] contained mostly spherical and few discoidal HDL particles, and apoE4[Phe265Ala] contained

62 In terms of discoidal HDL particles, there has been a debate as to t

63 and "hairpin" models of apoA-I structure in discoidal HDL particles.

64 n HDL, we generated highly defined benchmark discoidal HDL particles.

65 gests the role of apoA-I in the formation of discoidal HDL particles.

66 ereas the apoA-I[Delta(89-99)] mutant formed discoidal HDL particles.

67 Discoidal HDL prepared with apoA-I containing a Met-148-

68 a relatively large boundary layer in smaller discoidal HDL promotes preferential distribution of phos

69 ts of salt, pH, and point mutations on model discoidal HDL reconstituted from human apolipoprotein C-

70 ed spherical HDL by incubating reconstituted discoidal HDL with physiological plasma-remodeling enzym

71 HDL species involving spontaneous fusion of discoidal HDL with spherical HDL and subsequent fission.

72 rface curvature during conversion of nascent discoidal HDL(A-I) and HDL(A-II) containing either apoA-

73 and carboxyl-terminal deletion mutant formed discoidal HDL, and a carboxyl-terminal deletion mutant f

74 ydrophobic when apoA-I was incorporated into discoidal HDL, and Tyr(192) of HDL-associated apoA-I was

75 we propose a detailed model for the smallest discoidal HDL, consisting of two apoA-I molecules wrappe

76 After attachment of LCAT to discoidal HDL, the helix 5/5 domains in apoA-I form amph

77 ion on the spatial organization of apoA-I in discoidal HDL, we engineered three separate cysteine mut

78 n (HDL) region and promoted the formation of discoidal HDL, whereas the apoE4-mut1 did not displace a

79 at least two sizes of relatively homogeneous discoidal HDL-like particles depending on the initial li

80 ith phospholipids to produce nanometer-scale discoidal HDL-like particles.

81 eas apoA-I[delta(1-41)delta(185-243)] formed discoidal HDL.

82 enhances net cholesterol efflux mediated by discoidal HDL.

83 sterol in VLDL and HDL, and the formation of discoidal HDL.

84 DL particles, and apoE4[Phe265Ala] contained discoidal HDL.

85 the structure, stability, and remodeling of discoidal HDLs reconstituted from human apolipoproteins

86 2-hydroxypropyl-beta-cyclodextrins and with discoidal high density lipoprotein (HDL) particles to pr

87 Apolipoprotein A-I (apoA-I) readily forms discoidal high density lipoprotein (HDL) particles with

88 The structure of apoA-I on discoidal high density lipoprotein (HDL) was studied usi

89 lations on a series of progressively smaller discoidal high density lipoprotein particles produced by

90 lano) and apo A-I(R151C)(Paris), to lipid in discoidal high-density lipoprotein (HDL) particles are p

91 ls for apolipoprotein A-I (apo A-I) bound to discoidal high-density lipoprotein (HDL) particles, base

92 l-atom model for apolipoprotein (apo) A-I in discoidal high-density lipoprotein in which two monomers

93 ave been carried out on three separate model discoidal high-density lipoprotein particles (HDL) conta

94 icles by apolipoprotein A-I (apoA-I) to form discoidal high-density lipoproteins (rHDL) was dramatica

95 LCAT by apolipoprotein (apo) A-I on nascent (discoidal) high-density lipoproteins (HDL) is essential

96 ganization of apolipoprotein A-I (apoA-I) in discoidal, high-density lipoprotein (HDL) complexes with

97 with the three full-length apoE isoforms are discoidal in shape, and structurally indistinguishable.

98 Predominately discoidal in shape, these particles have diameters betwe

99 that reconstituted apoA-I/HDL particles are discoidal in shape.

100 ituted apoA-I/HDL particles, in general, are discoidal in shape.

101 asma HDL and apoA-I levels and converted the discoidal into spherical HDL, indicating that the LCAT a

102 nts with LCAT deficiency have abnormal small discoidal LDLs and HDL particles, and develop kidney fai

103 They are discoidal lipid bilayer fragments encircled and stabiliz

104 (apo A-I) and its engineered constructs form discoidal lipid bilayers upon interaction with lipids in

105 ccumulates in the medium as apolipoprotein E-discoidal lipid particles.

106 Bicelles are bilayered discoidal lipid-detergent assemblies which are useful as

107 ly process, we prepared soluble monodisperse discoidal lipid/protein particles with controlled size a

108 o main competing models for the structure of discoidal lipoprotein A-I complexes both presume that th

109 complex provides a high-resolution view of a discoidal lipoprotein particle in which all of the inter

110 ive to the lipid bilayer was investigated in discoidal lipoprotein particles made with 1-palmitoyl-2-

111 a second apolipoprotein residing in the same discoidal lipoprotein.

112 id along the periphery of the bilayer of the discoidal lipoprotein.

113 olesterol efflux and esterification in model discoidal lipoproteins (including reduced protein size,

114 ts shows that protein oxidation destabilizes discoidal lipoproteins and accelerates protein unfolding

115 ine the number of apo A-I molecules bound to discoidal lipoproteins and compare this with values obta

116 re in solution facilitates reconstitution of discoidal lipoproteins but has no significant effect on

117 Using discoidal lipoproteins made with a combination of apolip

118 cture in protein-lipid interactions, we used discoidal lipoproteins reconstituted from dimyristoylpho

119 e lipoproteins as well as being able to form discoidal lipoproteins upon incubation with either lipos

120 fide form of the mutant was not able to form discoidal lipoproteins with liposomes of either dimiryst

121 on in the formation and kinetic stability of discoidal lipoproteins, thermal unfolding and refolding

122 mutant, as determined by its ability to form discoidal lipoproteins, was nearly identical to that of

123 by fluorescence resonance energy transfer in discoidal lipoproteins.

124 oLp-III with the phospholipid acyl chains in discoidal lipoproteins.

125 as incorporated into soluble nanometer scale discoidal membrane bilayers (nanodiscs), and potentials

126 teristic of plasma apolipoproteins and forms discoidal micelles with phosphatidylcholine.

127 s or native lipoproteins but interacted with discoidal micelles.

128 ins upon incubation with either liposomes or discoidal micelles.

129 The iNPG is a discoidal micrometer-sized particle that can be loaded w

130 using v-SNARE-reconstituted 23-nm-diameter discoidal nanolipoprotein particles (vNLPs) as fusion pa

131 Nanodiscs are examples of discoidal nanoscale lipid-protein particles that have be

132 Nanodiscs are an example of discoidal nanoscale self-assembled lipid/protein particl

133 ormation of two populations of FC-containing discoidal nascent HDL particles.

134 ubilizes the exovesiculated domain to create discoidal nascent HDL particles.

135 Like 2F, 4F also forms discoidal nascent high density lipoprotein-like particle

136 The peptide Ac-18A-NH2 forms discoidal nascent high density lipoprotein-like particle

137 l structure, independent of whether it forms discoidal or vesicular complexes.

138 olipoprotein AI (423:74:1 mol/mol) forming a discoidal particle 360 A in diameter and 45 A thick; the

139 stabilization of the ApoA1-POPC-cholesterol discoidal particle and allows for a more optimal lipid p

140 -I) and phospholipid (apoA-I/HDL) has been a discoidal particle approximately 100 A in diameter and t

141 edominantly high levels of apoA-I containing discoidal particles and had an increased prebeta1-HDL/al

142 able to transform phospholipid vesicles into discoidal particles but at a 3-fold reduced rate compare

143 ployed to self-assemble soluble monodisperse discoidal particles called Nanodiscs.

144 xpressed in ldlA-7 cells using reconstituted discoidal particles consisting of apoE, 1-palmitoyl-2-ol

145 macrophages, 2) apolipoprotein E only formed discoidal particles following macrophage cholesterol enr

146 lts indicate that the formation of apoE.DMPC discoidal particles occurs in a series of steps.

147 n morphology (lamellar/vesicular and stacked discoidal particles reminiscent of those in lecithin/cho

148 oE with phospholipid and cholesterol to form discoidal particles that floated at densities of 1.08-1.

149 Adsorption of these discoidal particles to clean hydrophilic glass (or silic

150 imyristoylphosphatidylcholine (DMPC) to form discoidal particles was investigated by introducing sing

151 lipoproteins (lamellar/vesicular and stacked discoidal particles), occlusive coronary atherosclerosis

152 in size and morphology and included numerous discoidal particles, mimicking those observed in LCAT-de

153 ion with DMPC liposomes, and the size of the discoidal particles.

154 inside the pores of quasi-hemispherical and discoidal particles.

155 tent was somewhat less than in reconstituted discoidal PC.apoA-I complexes for all apoA-I variants, s

156 Conversion of discoidal phospholipid (PL)-rich high density lipoprotei

157 ccumulate within the hydrophobic core of the discoidal phospholipid bilayer transforming it into a sp

158 esteryl ester molecules in the middle of the discoidal phospholipid bilayer.

159 in the lipid-free state and in reconstituted discoidal phospholipid-cholesterol-apoA-I particles (rHD

160 A1 transporter function have only very small discoidal prebeta-1 HDL, and develop hepatosplenomegaly,

161 lity to transform phospholipid vesicles into discoidal protein-lipid complexes and that Thr-31 is a k

162 ing membrane patches in the form of nanosize discoidal proteolipid particles or "native nanodiscs." U

163 erase (LCAT), to form cholesteryl ester, the discoidal r-HDL became spheroidal.

164 In discoidal r-HDL, we found that POPC >/= DOPC = PAPC/DPPC

165 r-HDL were hydrolyzed at a faster rate than discoidal r-HDL.

166 choline (DMPC) binding kinetics, and size of discoidal reconstituted high-density lipoprotein (rHDL)

167 ion of rotational alignment was observed for discoidal red blood cells.

168 Discoidal rHDL particles containing two lipid-bound apoA

169 "fixed helix-helix registry." Additionally, discoidal rHDL were transformed in vitro to core-contain

170 These discoidal shaped cells underwent a dramatic form of acti

171 The structure showed a discoidal-shaped LDL particle with high-density regions

172 can convert multilamellar DMPC vesicles into discoidal-shaped particles.

173 (CD44TA) targeting moiety were conjugated to discoidal silicon mesoporous microparticles (SMP) to enh

174 antibody-labeled and energy-focusing porous discoidal silicon nanoparticles (nanodisks) and high-thr

175 l segments was incorporated into each of the discoidal species.

176 Lenticulatheca encompasses discoidal sporangia containing monads formed from dyads

177 Early studies of rHDL suggested a discoidal structure, which included pairs of antiparalle

178 al region of apoA-I binds lipid and can form discoidal structures and a heterogeneous population of v

179 ot perturb their ability to form homogeneous discoidal structures.

180 Conversion from a discoidal to a saddle-shaped particle involves loss of h

181 de that as lipid-bound apoA-I adjusts from a discoidal to a spherical surface its intermolecular inte

182 tion of high-density lipoproteins (HDL) from discoidal to spherical particles.

183 00 microm(2)), exocytosis of a population of discoidal vesicles located in the apical cytoplasm of th