ライフサイエンスコーパス: dishevelled

1 ovide evidence that Seahorse associates with Dishevelled.

2 n a process that requires the recruitment of Dishevelled.

3 downstream of heterotrimeric G proteins and Dishevelled.

4 regulation of cell polarity by Frizzled and Dishevelled.

5 eta-catenin, possibly via targets other than Dishevelled.

6 regulates canonical Wnt signaling by binding Dishevelled.

7 nt pathway, providing a link between Wnt and Dishevelled.

8 FZD2 to interact with its downstream target DISHEVELLED.

9 on of CK2 reduces levels of beta-catenin and dishevelled.

10 he DIX domain of the Wnt signalling molecule dishevelled.

11 signaling, probably due to interaction with dishevelled.

12 2 reporter was mediated by the DEP domain of Dishevelled.

13 ssociated with the PCP proteins Frizzled and Dishevelled.

14 n signalling associated with hyper-activated Dishevelled-1 (Dvl-1) localized to the basal body.

15 We show that loss of function of Dishevelled-1 (Dvl1) mimics and enhances the Wnt7a pheno

16 teraction of release machinery proteins with Dishevelled-1 (Dvl1), a scaffold protein that determines

17 Here, we report unexpected roles of Dishevelled 2 (Dvl2) and interphase polo-like kinase 1 (

18 Here, we show that Dishevelled 2 (Dvl2), a key component of the Wnt signali

19 estingly, as embryos reach blastocyst stage, Dishevelled 2 becomes increasingly associated with cell

20 and thus indirectly attenuates inhibition of Dishevelled 2 in the Wnt/beta-catenin pathway.

21 In these cells, the core PCP molecule Dishevelled 2, the BB/spindle positioning protein Dlg3,

22 is not required for membrane recruitment of Dishevelled 2; Ptk7 and Frizzled3/Frizzled6 receptors ac

23 Knockdown of Dishevelled-2 (Dvl-2) and Dvl-3 also suppressed neurite

24 a-arrestin recruitment and activation of the dishevelled-2 (Dvl-2) scaffold and not by G protein alph

25 components of the Wnt pathway, specifically Dishevelled-2 (Dvl2) expression, resulting in diminished

26 Coil Kinase 1 (ROCK1) activity downstream of Dishevelled-2 (Dvl2).

27 romotes Frizzled1 endocytosis, downregulates Dishevelled-2 protein, and inhibits Wnt3A-stimulated bet

28 iscuity in five PDZ domains: the human Dvl2 (Dishevelled-2) PDZ domain, the human Erbin PDZ domain, t

29 through the canonical Wnt pathway involving dishevelled-2, beta-catenin and survivin.

30 Previously we determined that Dishevelled-2/3 (Dvl) mediate Wnt-3a-dependent neurite o

31 PDZRN3 ubiquitinates Dishevelled 3 (Dvl3), to promote endocytosis of the Friz

32 brane in trophectoderm cells, while at E4.5, Dishevelled 3 is highly enriched in the cytoplasm of ICM

33 the N-terminal domain of Dab2 interacts with Dishevelled-3 (Dvl-3), a signaling mediator of the Wnt p

34 Dishevelled-3 (Dvl3), a key component of the Wnt signali

35 on assays and induces the phosphorylation of Dishevelled, a cytoplasmic component downstream of Frizz

36 Dishevelled, a phosphoprotein scaffold, is a central com

37 signaling proteins Frizzled, Strabismus, and Dishevelled act in concert with the small GTPase Rac1 to

38 (dsh1), we show that this mutation inhibits Dishevelled activation of Rac, regardless of its subcell

39 ty is not essential in the pathway, and that dishevelled acts downstream of Xnr3 and not in a paralle

40 Dishevelled also has important roles in the governance o

41 vertebrate homologue of Lgl associates with Dishevelled, an essential mediator of Wnt signalling, an

42 has been identified by its association with Dishevelled, an intracellular component of Wnt signal tr

43 oval of positive regulators of Wg signaling, dishevelled and armadillo, results in rapid SSC loss.

44 We demonstrate that DVL dishevelled and axin (DIX) domain, but not DIX domain-me

45 antibody is confirmed by down-regulation of Dishevelled and beta-catenin.

46 lls, where it can be found in a complex with dishevelled and beta-catenin.

47 ing activity is dependent on the presence of Dishevelled and blocked in the absence of Dynamin and Ra

48 WGEF protein can bind to Dishevelled and Daam-1, and deletion of the Dishevelled-

49 d for Wnt/beta-catenin signaling upstream of Dishevelled and downstream of the Wnt ligand.

50 gnaling in endothelial cells, as measured by Dishevelled and ERK1/2 phosphorylation, and inhibition o

51 oles in planar polarity signaling, including Dishevelled and Frizzled-7 and -8.

52 Finally, accumulation of Dishevelled and Inturned near the basal apparatus of cil

53 tionship between subcellular localization of Dishevelled and its signaling activities remains unclear

54 e show that maternal JNK activation requires Dishevelled and JNK is enriched in the nucleus of Xenopu

55 at could involve direct interactions between Dishevelled and Notch at the cell cortex.

56 closure fails in both mouse and Xenopus when dishevelled and other components of the planar-cell-pola

57 embrane by Frizzled 3, formed a complex with Dishevelled and phosphorylated Dsh in vitro.

58 We show that Strabismus binds directly to Dishevelled and Prickle and is able to recruit them to m

59 ismus activity, the planar polarity proteins Dishevelled and Prickle are mislocalised in the cell.

60 In the second phase, Dishevelled and Prickle are required for these proteins

61 CP interactions, including interactions with Dishevelled and Prickle that function in a context-depen

62 itment of planar polarity proteins including Dishevelled and Prickle.

63 Dishevelled and RhoA act downstream of Wnt11-Ror2 to reg

64 f beta-catenin-independent signaling through Dishevelled and Ror2 causes predictable changes in premi

65 he Wnt pathway components Silberblick/Wnt11, Dishevelled and Strabismus.

66 y protein Daam1 as an important link between Dishevelled and the Rho GTPase for cytoskeletal modulati

67 larized distribution of the core PCP protein Dishevelled and to position the primary cilium at the ab

68 ne, and Frizzled-7 induced colocalization of Dishevelled and WGEF.

69 or Wnt5a activation of p38; siRNAs targeting Dishevelleds and expression of the Dishevelled antagonis

70 ream signaling molecules (i.e., Frizzled and Dishevelled) and moreover may provide a valuable means o

71 ventricles, PCP regulators (e.g., Vangl2 and Dishevelled), and cytoskeletal networks in the establish

72 receptor-related proteins 5 and 6 (LRP5/6), Dishevelled, and Axin signalosome assembly and provide a

73 thways share the effector proteins frizzled, dishevelled, and beta-arrestin.

74 ecruited to the posterior cortex by Frizzled/Dishevelled, and in turn polarizes the apical-basal pola

75 PSD-95, Syntenin, Erbin, GRIP, NHERF, Inad, Dishevelled, and Shank).

76 nsity lipoprotein receptor-related protein), dishevelled, and the glycogen synthase kinase shaggy (GS

77 targeting Dishevelleds and expression of the Dishevelled antagonist Dapper-1 do not suppress the p38

78 oter, demonstrating that Dapper is a general Dishevelled antagonist.

79 PAR-1 phosphorylation sites in Dishevelled are required for this translocation, but not

80 Dishevelleds are modular proteins that lie at the crossr

81 Thus, we identify a dual function for Dishevelled as an inhibitor of Notch signalling and an a

82 CXXC-type zinc finger protein 5 (CXXC5) with Dishevelled as one such negative regulator of WNT in hai

83 e have renamed WID (WT1-induced Inhibitor of Dishevelled), as a novel WT1 transcriptional target that

84 s with functional reporter assays identified dishevelled associated activator of morphogenesis 1 (DAA

85 eracting with its downstream effector Daam1 (Dishevelled associated activator of morphogenesis 1), an

86 Dishevelled-associated activator of morphogenesis 1 (Daa

87 a constitutively active C-terminal domain of Dishevelled-associated activator of morphogenesis 1 (DAA

88 Daam1 (dishevelled-associated activator of morphogenesis-1) is

89 upon Wnt3a stimulation by beta-arrestin- and Dishevelled-associated kinases.

90 hown very recently to bridge Wnt-induced and Dishevelled-associated PtdIns(4,5)P2 production to the p

91 We show that Dishevelled-associating protein with a high frequency of

92 Levels of Dishevelled at junctions are regulated by a Cullin-3-Dia

93 Rpgrip1l was also essential for stabilizing dishevelled at the cilium base in the zebrafish floor pl

94 fied CamKII as an interaction partner of the dishevelled-beta-arrestin protein complex by quantitativ

95 ifferent Dvl proteins and the composition of dishevelled-beta-arrestin protein complexes contribute t

96 We hypothesized that the composition of dishevelled-beta-arrestin protein complexes contributes

97 lled via its C-terminal CXXC zinc finger and Dishevelled binding domains and potently inhibits WNT/be

98 nous-related formin first studied as a novel dishevelled binding protein and shown to be crucial for

99 Dishevelled and Daam-1, and deletion of the Dishevelled-binding domain generates a hyperactive from

100 trigger non-canonical Wnt signaling via the Dishevelled-binding protein, Daple.

101 Mechanistically, Dishevelled binds and directly inhibits CSL transcriptio

102 onal analyses demonstrate that regulation of Dishevelled by this ubiquitin ligase antagonizes the Wnt

103 oth membrane-targeted and cytoplasm-targeted Dishevelled can potently activate canonical Wnt signalin

104 , an intracellular phosphoprotein that binds Dishevelled, catenins, and other signaling proteins, is

105 , RNA interference (RNAi) of beta-catenin or dishevelled causes the inappropriate regeneration of a h

106 s for apical-basal polarity and the Frizzled/Dishevelled complex for PCP), a diverse array of polariz

107 P signaling through Knypek (Glypican4/6) and Dishevelled components.

108 n and focus on the biological processes that Dishevelled controls.

109 me, and, in this complex, Rpgrip1l prevented dishevelled degradation.

110 enin stability, our data on the stability of Dishevelled demonstrates that two distinct ubiquitin lig

111 n, as does suppression of the phosphoprotein Dishevelled, demonstrating that casein kinase 2 is downs

112 icted junctional domains containing Frizzled/Dishevelled/Diego (Fz/Dsh/Dgo) or Van Gogh/Prickle (Vang

113 n-canonical Wnt signaling is blocked using a Dishevelled dominant-negative protein, gastrulation move

114 Frizzed-7 Wnt receptor and the PDZ domain of Dishevelled, down-regulating canonical Wnt signaling and

115 Both pathways require members of the Dishevelled (Dsh or Dvl) family of cytoplasmic factors f

116 We identified peptide aptamers against Dishevelled (Dsh) and beta-catenin (beta-cat) to target

117 Armadillo (Arm)/beta-catenin and Dishevelled (Dsh) are key components of Wnt signal trans

118 calization of LIN-17/Frizzled (Fz) and MIG-5/Dishevelled (Dsh) during the B cell division.

119 itive to dgo dosage or dominantly modified a dishevelled (dsh) GOF phenotype, supporting a role in Fz

120 Dishevelled (Dsh) is a key signaling molecule in the can

121 strate that dAbl positively regulates the Fz/Dishevelled (Dsh) PCP pathway without affecting canonica

122 Although the intracellular protein Dishevelled (Dsh) plays a dual role in both the Wnt-beta

123 We demonstrate that Dishevelled (Dsh) plays an important role in mediating t

124 ll movement through its association with the Dishevelled (Dsh) protein.

125 Dishevelled (Dsh) proteins DSH-1 and MIG-5 redundantly m

126 olarity (PCP) gene products prickle (pk) and dishevelled (dsh) show M/L polarization.

127 vident that a noncanonical Wnt/Frizzled (Fz)/Dishevelled (Dsh) signalling pathway, which is related t

128 of APC/C function leads to reduced levels of Dishevelled (Dsh), a core PCP factor.

129 ivisions proceed normally in embryos lacking dishevelled (dsh), a key component of the PCP pathway.

130 rbs CE and disrupts membrane localization of Dishevelled (Dsh), a key regulator of PCP and CE.

131 We show that dishevelled (Dsh), a key signaling protein, is required

132 choline receptors with weak interaction, (b) dishevelled (Dsh), and (c) another four proteins, Rapsyn

133 the Wnt receptor-associated scaffold protein Dishevelled (Dsh), but the mechanism by which Nkd inhibi

134 n bind and inhibit the Wnt signal transducer Dishevelled (Dsh), but the mechanism by which Nkd limits

135 s involved in PCP, such as Frizzled (Fz) and Dishevelled (Dsh), but there is also some evidence that

136 With the exception of dishevelled (dsh), the genes functioning in these two pa

137 luding frizzled (fz), flamingo/starry night, dishevelled (dsh), Van Gogh/strabismus and prickle, func

138 CP) pathway, together with Frizzled (Fz) and Dishevelled (Dsh).

139 ugh an interaction with the scaffold protein Dishevelled (Dsh).

140 gnaling by binding the intracellular protein Dishevelled (Dsh).

141 tself interacts with the Wnt pathway protein Dishevelled (Dsh).

142 d can colocalize with the signaling molecule Dishevelled (Dsh).

143 e cells depends critically on the ability of Dishevelled (Dsh)/Dvl proteins to interpret distinct typ

144 Removal of two dishevelleds (dsh-2 and mig-5) leads to a global loss of

145 and a 'PCP-core' including Frizzled(Fz) and Dishevelled(Dsh) contribute to polarization of individua

146 Dishevelled (Dvl or Dsh) signals downstream of Wnt recep

147 However, FBM neurons migrated normally in Dishevelled (Dvl) 1/2 double mutants, and in zebrafish e

148 e Lys63-linked polyubiquitin chains modulate Dishevelled (Dvl) activity upstream in the Wnt/beta-cate

149 rodent embryo fibroblasts, we show here that dishevelled (Dvl) and axin, two major components of the

150 ceptor complex leading to the recruitment of Dishevelled (Dvl) and Axin1 to the plasma membrane.

151 s was conducted to confirm the expression of Dishevelled (Dvl) and cytosolic beta-catenin in matched

152 ty (PCP) pathway, through the recruitment of Dishevelled (Dvl) and Dvl-associated activator of morpho

153 This effect is mimicked by the expression of Dishevelled (Dvl) and is blocked by Sfrp1, a secreted Wn

154 The cytoplasmic protein Dishevelled (Dvl) and the associated membrane-bound rece

155 2) Par1b attenuates the effect of Dishevelled (Dvl) expression, an inducer of wnt signalin

156 rane recruitment of the cytoplasmic effector Dishevelled (Dvl) is a critical step in Wnt/beta-catenin

157 Dishevelled (Dvl) is a key component of Wnt/beta-catenin

158 Dishevelled (Dvl) is a key molecule downstream of Fz rec

159 Dishevelled (DVL) is a key scaffolding protein and a bra

160 Dishevelled (Dvl) is a scaffold protein that serves as t

161 Dishevelled (Dvl) is an essential protein in the Wnt sig

162 Dishevelled (DVL) is associated with axonal microtubules

163 ociliary epithelial development to show that Dishevelled (Dvl) is essential for the apical positionin

164 The intracellular signaling molecule Dishevelled (Dvl) mediates canonical and non-canonical W

165 trulation, the non-canonical Wnt pathway via Dishevelled (Dvl) orchestrates the activities of the GTP

166 Recently, we reported Wnt signaling and dishevelled (Dvl) overexpression in malignant pleural me

167 ow that the Wnt pathway is activated through Dishevelled (Dvl) overexpression in NSCLC.

168 SIRT1 regulates Dishevelled (DVL) protein levels in cancer cells, and DV

169 Dishevelled (Dvl) protein plays a central role in this p

170 accumulation of PCP core components, such as Dishevelled (Dvl) protein, is associated with PCP functi

171 elated protein 6 (Lrp6), and the cytoplasmic dishevelled (Dvl) protein.

172 Because Dishevelled (Dvl) proteins are effectors of both canonic

173 Dishevelled (Dvl) proteins are intracellular effectors o

174 Dishevelled (DVL) proteins serve as crucial regulators t

175 nificant decrease in the levels of all three Dishevelled (Dvl) proteins.

176 thereby recruiting the cytoplasmic molecule dishevelled (Dvl) to the plasma membrane.Frizzled-mediat

177 Dishevelled (Dvl) transduces Wnt signals from the recept

178 Dishevelled (Dvl), a cytoplasmic protein downstream of F

179 Here we uncover an unexpected role of Dishevelled (DVL), a positive Wnt regulator, in promotin

180 re that in muscle cells, MuSK interacts with Dishevelled (Dvl), a signaling molecule important for pl

181 tumor cell lines was mediated by Frizzled3, Dishevelled (Dvl), and c-Jun N-terminal kinase.

182 sembly by controlling the phosphorylation of Dishevelled (DVL), another ciliogenesis regulator.

183 recruitment of shared components, including dishevelled (Dvl), axin, and glycogen synthase kinase 3

184 PCP proteins, including Van Gogh (Vang) and Dishevelled (Dvl), segregate into distinct complexes on

185 tream regulator of this signaling pathway is Dishevelled (Dvl), which, among other multiple interacti

186 matin array data, we identified the protein, dishevelled (DVL)-2, as being regulated in the nucleus a

187 tenin signaling requires the adapter protein Dishevelled (Dvl).

188 on of convergent extension (CE) movements is Dishevelled (Dvl).

189 o binding but rather by its interaction with Dishevelled (Dvl).

190 trin (DEP) domain of the Wnt pathway protein Dishevelled (Dvl).

191 by WNTs and regulated by the phosphoprotein Dishevelled (DVL).

192 thways in vertebrates use the phosphoprotein Dishevelled (Dvl).

193 s the cytoplasmic effectors Prickle (Pk) and Dishevelled (Dvl).

194 a complex partly by limiting the activity of dishevelled (DVL).

195 Dishevelled (Dvl/Dsh) protein is one of key components i

196 lity of the crucial Wnt signalling effector, dishevelled (Dvl2), and impairing Wnt signalling.

197 ate the K63-linked polyubiquitination of the Dishevelled, Egl-10 and Pleckstrin (DEP) domain of the W

198 bitory function of DSH-1, which requires its dishevelled, Egl-10, and pleckstrin (DEP) domain, acts b

199 This translocation requires the Dishevelled, Egl-10, Pleckstrin (DEP) domain, but the mo

200 the interface between Gbeta5 and N-terminal Dishevelled, EGL-10, Pleckstrin/DEP Helical Extension (D

201 cking the entire N-terminal domain (i.e. the dishevelled/EGL-10/pleckstrin domain plus intervening do

202 d) < 10 nm), but constructs lacking the DEP (dishevelled/EGL-10/pleckstrin) domain bound with much lo

203 es significant conformational changes in the dishevelled/Egl/pleckstrin (DEP) domain, a conserved str

204 DME cells of embryos mutant for wingless or dishevelled exhibit polarisation defects and fail to clo

205 We identify mig-5, a Dishevelled family member, as a gene that regulates seve

206 We report the interaction of LRRK2 with the dishevelled family of phosphoproteins (DVL1-3), key regu

207 ed receptors and cytoplasmic proteins of the Dishevelled family; however, there has been some dispute

208 The C3 VCD competes with Dishevelled for binding to the DIX domain of Axin1, whic

209 s block the dissociation of the Wnt effector Dishevelled from nucleoredoxin.

210 Removal of Dishevelled from the cell membrane disrupts convergent e

211 n to be an important switch in regulation of Dishevelled function and Wnt signaling.

212 P pathway, but, importantly, may not require Dishevelled function.

213 Dishevelled genes (Dvls) are essential for Wnt signaling

214 When Dishevelled-GFP fusion proteins are overexpressed in sin

215 enin pathway in CML CD34+ cells by depleting dishevelled homolog 3 (DVL3).

216 Here, we describe a role for a C. elegans Dishevelled homolog, DSH-2, in an asymmetric neuroblast

217 The three C. elegans Dishevelled homologs contribute to these processes in di

218 ng mice with null mutations in two mammalian Dishevelled homologs, Dvl1 and Dvl2, that during neurula

219 sttranslationally and relies on frizzled and dishevelled homologs, which also control POP-1 asymmetry

220 E3 ubiquitin ligase complex is recruited to Dishevelled in a Wnt-dependent manner that promotes its

221 nt to account for the pathway specificity of Dishevelled in the canonical Wnt versus PCP signaling ca

222 we have identified proteins associated with Dishevelled, including a Cullin-3 ubiquitin ligase compl

223 hese novel results are the first to detail a Dishevelled-independent Wnt response, demonstrating a cr

224 fic for the Wnt receptor signaling component Dishevelled induced lysosomal fusion with ehrlichial inc

225 d in the presence of Wnt whereas the tuberin-Dishevelled interaction was increased.

226 some dispute regarding whether the Frizzled-Dishevelled interactions are the same in both cases.

227 plying that qualitatively different Frizzled-Dishevelled interactions underlie canonical signaling.

228 e view that qualitatively different Frizzled-Dishevelled interactions underlie planar polarity and ca

229 Dishevelled is a conserved protein that interprets signa

230 ta demonstrate that membrane localization of Dishevelled is a prerequisite for vertebrate PCP signali

231 Dishevelled is an important transducer of divergent Wnt

232 localizes at the anterior cell edge, whereas Dishevelled is enriched posteriorly.

233 lasmic region of RNF43 and the PDZ domain of dishevelled is essential for this suppression.

234 Dishevelled is not required for Wnt5a activation of p38;

235 In the present study, we report that Dishevelled is post-translationally modified, both in vi

236 lin-44 and egl-20, lin-17/Frizzled and dsh-1/Dishevelled, is compromised, synapses develop ectopicall

237 ciliopathy protein complex that finely tunes dishevelled levels, thereby modulating planar cell polar

238 The DEP domain of dishevelleds mediates planar polarity of cells within a

239 erases 1 and 7 as the key enzymes catalyzing Dishevelled methylation.

240 F9 teratocarcinoma cells results in reduced Dishevelled methylation.

241 , and is required for local concentration of Dishevelled (MIG-5) in the ABar cortex adjacent to C.

242 The DIX domain of dishevelleds modulates a beta-catenin destruction comple

243 xpression of a dominant negative Wnt11 and a Dishevelled mutant Xdd1.

244 Corresponding Xenopus Dishevelled mutants show compromised ability to interfer

245 Dapper also inhibits activation by Dishevelled of c-Jun N-terminal kinase (JNK), a componen

246 ates with greater colocalized Wg, Arrow, and Dishevelled on endosomes.

247 does not alter the total cellular levels of Dishevelled or Flamingo, but only that of the junctional

248 onents of the canonical Wnt pathway, such as dishevelled or Tcf, also results in ventrally curved sal

249 ified aberrant Wnt activation as a result of Dishevelled overexpression in malignant mesothelioma.

250 t5a-dependent processes in vivo and identify Dishevelled phosphorylation as a physiological target of

251 suggesting that differential methylation of Dishevelled plays an important role in Wnt signaling.

252 Through its many signaling activities, Dishevelled plays important roles in the embryo and the

253 catenin complexes (AGbetaC) as Wnt decreases Dishevelled/PLIN2 but increases AGbetaC/PLIN2 associatio

254 dimerization provides cross-linking between Dishevelled polymers, illustrating a key principle under

255 In contrast the cytoplasmic components Dishevelled, Prickle and Diego are not needed for interc

256 is promoted by the core proteins Strabismus, Dishevelled, Prickle, and Diego.

257 veloping new reagents to sequester wild-type Dishevelled protein either at the cell membrane or away

258 The Dishevelled protein mediates several diverse biological

259 The Dishevelled protein regulates many developmental process

260 inhibits cell movements and localization of Dishevelled protein to the cell cortex, processes associ

261 ling, suggesting that local concentration of Dishevelled protein, but not its spatial localization, i

262 Here we show that three of the mouse Dishevelled proteins are not only expressed in oocytes a

263 of the beta-catenin destruction complex and dishevelled proteins in vivo and is recruited to membran

264 act differentially with the three vertebrate dishevelled proteins.

265 milarly, the methylation-deficient mutant of Dishevelled, R271K, displayed spontaneous membrane local

266 ellular localization of the core PCP protein Dishevelled, recruiting it to Rab8-positive vesicles and

267 regions of the Frizzled receptor that block Dishevelled recruitment.

268 sential mediator of Wnt signalling, and that Dishevelled regulates the localization of Lgl in Xenopus

269 Dishevelled relays Wnt signals from the plasma membrane

270 eads to the activation of a noncanonical Wnt/dishevelled signal transduction pathway that cooperates

271 These findings suggest that Wnt5a-Ror-Dishevelled signaling constitutes a core noncanonical Wn

272 Finally, we show that blocking noncanonical dishevelled signaling in muscle fibers disrupts AChR pre

273 Dishevelled signaling plays a critical role in the contr

274 ell killing induced by either Wnt-1 siRNA or Dishevelled siRNA in these cells.

275 Both Wnt-1 small interfering RNA (siRNA) and Dishevelled siRNA induced significant apoptosis in these

276 Rpgrip1l in planar polarity was mediated by dishevelled stabilization.

277 Studies looking at mutated forms of Dishevelled suggested that stable recruitment of Disheve

278 phates [PtdIns (4,5)P2] through frizzled and dishevelled, the latter of which directly interacted wit

279 with Dkk2C unmasked its ability to signal to Dishevelled through multiple frizzleds, indicating that

280 that the key modular intracellular mediator Dishevelled, through a specific activation of RhoA GTPas

281 Ryk also binds to Dishevelled, through which it activates the canonical Wn

282 Here, we show that Wnt acts via Dishevelled to inhibit Notch signalling, and that this c

283 evelled suggested that stable recruitment of Dishevelled to membranes by Frizzled was required only f

284 mponent of the Wnt-PCP pathway that connects Dishevelled to Rho activation.

285 At the molecular level, Gpr125 recruited Dishevelled to the cell membrane, a prerequisite for Wnt

286 Wnt5b stimulation, Fz2 but not Ryk recruits Dishevelled to the cell membrane, suggesting that Fz2 an

287 Asymmetrical localization of Prickle and Dishevelled to the opposite cell edges in zebrafish gast

288 n-4, two ciliopathy proteins known to target dishevelled to the proteasome, and, in this complex, Rpg

289 the Wnt/Frizzled pathway seems to occur from Dishevelleds to axin/glycogen synthase kinase 3(GSK3)/be

290 and in parallel to lin-17/frizzled and dsh-1/dishevelled, to determine the direction of neurite growt

291 g Frizzled, Van Gogh, Flamingo, Prickle, and Dishevelled, to establish their characteristic asymmetri

292 based assays, to show that the DEP domain of Dishevelled undergoes a conformational switch, from mono

293 nalysis demonstrated that WID interacts with Dishevelled via its C-terminal CXXC zinc finger and Dish

294 s the Wnt signalosome, which is assembled by Dishevelled via reversible head-to-tail polymerization b

295 Whereas the C terminus of Frodo binds Dishevelled, we demonstrate that the conserved N-termina

296 discovered an unexpected antagonism between Dishevelleds, which may function as a signal amplificati

297 ic aspects of CE that involve interaction of Dishevelled with mediators different from that of RhoA G

298 idue sequence motifs first recognized in fly Dishevelled, worm EGL-10, and mammalian Pleckstrin), we

299 ingly, within the same tissues where Xenopus Dishevelled (Xdsh) controls cell fate via canonical Wnt

300 ulation was disrupted by the manipulation of Dishevelled (Xdsh) signaling.