ライフサイエンスコーパス: divalent ion

1 here with little preference for a particular divalent ion.

2 ne hydroxyl of proteins in the presence of a divalent ion.

3 nnel of the trimeric Dut where it chelates a divalent ion.

4 als that the binding affinity is enhanced by divalent ion.

5 ut a single monomer binds in the presence of divalent ion.

6 ch contain not only monovalent ions but also divalent ions.

7 ree of esterification and in the presence of divalent ions.

8 regions through an "induced coalescence" of divalent ions.

9 the same whether supported by monovalent or divalent ions.

10 t cations, chloride, and to a lesser extent, divalent ions.

11 y complex but less so for the monovalent and divalent ions.

12 n their relative stability in monovalent and divalent ions.

13 tion in the nominal absence of extracellular divalent ions.

14 as a neutral pH optimum and does not require divalent ions.

15 unction of polyion charge in the presence of divalent ions.

16 ndent channel binding functions for external divalent ions.

17 tegrity and increased sensitivity to monoand divalent ions.

18 n the presence of 1.6 M NH4Cl and absence of divalent ions.

19 e C and N-terminal sites can also bind other divalent ions.

20 ither activating (Pb2+) or inhibitory (Mg2+) divalent ions.

21 o mica and imaged in situ in the presence of divalent ions.

22 monovalent cations at low levels of external divalent ions.

23 to GO functional group bridging with NOM and divalent ions.

24 the presence of Mg(2+) and in the absence of divalent ions.

25 ferentially stabilized by Mg(2+) and similar divalent ions.

26 e adsorption was reported in the presence of divalent ions.

27 hich we use to quantify the cross-linking by divalent ions.

28 terminal tail domain mediate crosslinking by divalent ions.

29 nserved nucleotides, acting independently of divalent ions.

30 this transition was found to be enhanced by divalent ions.

31 ions, but large deviations are observed for divalent ions.

32 protein, both in the presence and absence of divalent ions.

33 63, and D65, all have decreased affinity for divalent ions.

34 mersed in a mixed solution of monovalent and divalent ions.

35 er ions selectively when compared with other divalent ions.

36 This study compares alpha and beta divalent ion affinities in Na(+) and K(+) solutions.

37 ng calorimetry and chemical denaturation and divalent ion affinity by titration calorimetry.

38 ated within the E helix, strongly influences divalent ion affinity in the mammalian beta-PV isoform a

39 nts are conducted in K(+)-containing buffer, divalent ion affinity is markedly higher.

40 Interestingly, the divalent ion affinity of Phl p 7 is unexceptional.

41 e AB domain do not necessarily influence the divalent ion affinity of the CD-EF domain.

42 herefore, the impact of the L85F mutation on divalent ion affinity was examined in rat beta-PV, in th

43 These data suggest that parvalbumin divalent ion affinity, particularly that of rat alpha, c

44 lthough all four proteins display heightened divalent ion affinity, the increases are small.

45 whether Phl p 7 likewise exhibits anomalous divalent ion affinity, we have also characterized Bra n

46 placement of Ser-55 with aspartate heightens divalent ion affinity.

47 at alpha-parvalbumin substantially increases divalent ion affinity.

48 elix, which may contribute to the heightened divalent ion affinity.

49 s-suggesting that the AB domain can modulate divalent ion affinity.

50 However, in the absence of divalent ion, alterations at position 257 increase the e

51 complete absence of intra- and extracellular divalent ions, although shifted to higher osmolarities (

52 binding to simple dsRNAs is not regulated by divalent ion, analysis of the interaction of the isolate

53 ded cuts and were independent of pH, type of divalent ion and chromatin repeat length.

54 close vicinity can efficiently coordinate a divalent ion and hold the peptide in a favorable configu

55 In the presence of divalent ions and at low temperature, previous electron

56 ocytes were also sensitive to block by these divalent ions and by DIDS but the sensitivity of ClC-2 t

57 VrtC can function in the absence of divalent ions and can utilize similar naphthacenedione s

58 ndent docking versus approximately 1 for the divalent ions and Co(NH(3))(6)(3+).

59 e of a mutant in the presence and absence of divalent ions and compare it with previous divalent ion-

60 roup I ribozyme in the presence of mono- and divalent ions and PEG crowders of different molecular we

61 -charge density and the interactions between divalent ions and surface groups.

62 514) and Arg(517)-all coupled to active site divalent ions and the DNA motion.

63 This protease activity is regulated by divalent ions and thiol-disulfide conversion in vitro.

64 observed depending on nucleotide substrate, divalent ion, and pH.

65 duced by separation of the C-terminal tails, divalent ions, and reducing agents.

66 nding/unbinding in high monovalent salt with divalent ions, and to further interpret noted chromatin

67 d for GTP hydrolysis by the Rho GTPases, the divalent ion apparently participates in the GTPase react

68 In reactions with 5 mm Mg2+, other free divalent ions are not needed.

69 er and distribution of excess monovalent and divalent ions around a short RNA duplex.

70 letal CRC in experiments using both mono and divalent ions as current carriers.

71 er selenide nanocrystals using two different divalent ions as guest cations (Zn(2+) and Cd(2+)) and c

72 ce of protein structure, lipid demixing, and divalent ions, as well as the physiological implications

73 r biophysical experimentation suggested that divalent ions associate with the M-box RNA to promote a

74 lts may be extended to the emerging field of divalent ion-associated amyloidosis.

75 ence between reaction constants of different divalent ions at the ideal condition explains why not al

76 opposite ways, we postulate that binding of divalent ions at the two sites interact.

77 For divalent ions, at very high ion concentration, further a

78 smolarity, release events following entry of divalent ions (Ba2+ or Ca2+) were less frequent.

79 is suggested that a region of "intermediate" divalent ion binding affinity, in between highly ligated

80 differences beyond the binding site modulate divalent ion binding behavior.

81 have explored the linkage of monovalent and divalent ion binding in the folding of the P4-P6 domain

82 ant, the kappa-zeta element is shown to be a divalent ion binding pocket, indicating that this region

83 We have also examined the divalent ion binding properties of the alpha 94/98E vari

84 Using a gating mutant at the divalent ion binding site, we were able to characterize

85 the heavy and light chain of alpha(IIb), the divalent ion binding sites on alpha(IIb), and at a disul

86 from PB theory in competitive monovalent and divalent ion binding to a DNA duplex.

87 The effect of divalent ion binding to deionized bacteriorhodopsin (dI-

88 ely charged residues in the loop regions for divalent ion binding.

89 ion-binding motif contribute to the unusual divalent ion-binding behavior associated with the rat be

90 elds a K(Na) of 630 M(-1) and indicates that divalent ion-binding is positively cooperative.

91 f divalent ions and compare it with previous divalent ion-bound TmCorA structures.

92 low Km for ATP, (b) sensitivity to external divalent ions, (c) lack of desensitization/inactivation,

93 The presence of divalent ion Ca(2+) significantly hydrophobized biofilm,

94 he RNase H complexes formed with one or both divalent ions can be individually observed and character

95 Conversely, interactions with divalent ions can be used to tether headgroups in-plane,

96 ces in the system or a smaller radius of the divalent ions can cause a more abrupt compaction transit

97 interactions in AL-DNA systems are complex: divalent ions can mediate strong attractions between dif

98 under three states reveals that ligands and divalent ions can stabilize similar RNA global conformat

99 receptor potential melastatin 7 (TRPM7) is a divalent ion channel with a C-terminally located alpha-k

100 ikely to apply to other structurally similar divalent ion channels.

101 converges in the two buffer systems, as the divalent ion concentration approaches approximately 1 mM

102 urrent results suggest that changes in local divalent ion concentration in the ribosome could profoun

103 d solution properties, such as pH, salt, and divalent ion concentration on the turnover number of the

104 e fraction effect (AMFE) only when the total divalent ion concentration was 5 mM, consistent with a m

105 The divalent ion concentration was set at 100 mM, to compare

106 erplay between native tertiary interactions, divalent ion concentration, and non-native secondary str

107 vage was measured as a function of mono- and divalent ion concentration, temperature, and pH.

108 onformation as a function of either mono- or divalent ion concentration.

109 Changes in monovalent and divalent ion concentrations drive an abrupt switch betwe

110 lly active on its own at high monovalent and divalent ion concentrations, four protein subunits are a

111 ndirectly on the hFOB cells by reducing free divalent ion concentrations, whereas pamidronate and zol

112 ing preference is amplified at physiological divalent ion concentrations.

113 g experiments over a range of monovalent and divalent ion concentrations.

114 d when folding is initiated by monovalent or divalent ions, consistent with equilibrium measurements

115 in and critically depends on the presence of divalent ions, consistent with results from small-angle

116 Divalent ions counteract this decondensation effect by m

117 tyrosine-containing substrate and Mn as the divalent ion defined a ternary complex mechanism with AD

118 proach would be similar to the inhibition of divalent ion dependent strand transfer by HIV integrase

119 induced in minimal essential media and under divalent ion-depleting conditions; it also participates

120 By comparison, free levels of the divalent ion do not influence maximum turnover (kcat) an

121 , and ESI-MS titrations confirmed that these divalent ions do not appreciably bind to P7.

122 w that lactate, acting through extracellular divalent ions, dramatically increases activity of an aci

123 To examine divalent ion effects over a wide concentration range, ur

124 In this current study, ionic strength and divalent ion effects were probed.

125 Decreasing the size of the divalent ions enhances the electrostatic bending attract

126 However, RED using feed streams containing divalent ions experiences lower power densities because

127 ns; and single-channel conductance to Na+ in divalent-ion-free conditions.

128 Divalent ions fulfill essential cellular roles and are r

129 Divalent ions further compact the fiber by promoting ben

130 crotiter wells in the presence of fetuin and divalent ions in a carbohydrate-dependent manner.

131 s universal reliance, the functional role of divalent ions in promoting RNA catalysis is manifold.

132 the presence of carboxyl groups on ENPs and divalent ions in the solution plays a key role in contro

133 d conductance was inhibited by extracellular divalent ions including Ba(2+) (K(i) = 0.7 mM) and Ca(2+

134 We found that extracellular divalent ions, including Ca(2+), inhibit the permeation

135 However, it also binds other divalent ions, including Cd(2+), Cu(2+), Mn(2+), and Mg(

136 the HDV ribozyme can self-cleave by multiple divalent ion-independent and -dependent channels, and in

137 on ion-binding distribution reveals that the divalent ion-induced helix bending attraction may come f

138 ofibers are described, including pH control, divalent ion induction, and concentration.

139 In contrast to more acidic Me2+ divalent ion inhibitors of the high-affinity Mn2+ site,

140 ent carried by Na+ and Cs+ in the absence of divalent ions (Ins) also activated at more negative pote

141 nism, separate from fast adaptation, whereby divalent ions interacting with the local lipid environme

142 ripping voltammetric current response to the divalent ion is enhanced to achieve a subnanomolar detec

143 ke and release, but in this case the primary divalent ion is Zn(2+) rather than Ca(2).

144 Thus, the reduced affinity for divalent ions is evidently not the result of heightened

145 dsorption to negatively charged surfaces via divalent ions is extensively used in the study of biolog

146 anism for vimentin networks and suggest that divalent ions may help regulate the cytoskeletal structu

147 a 10-fold activity change of monovalent and divalent ions measured at room temperature, respectively

148 Divalent ions Mg(+2), Zn(+2), Co(+2), Hg(+2) and Cd(+2)

149 ity of Gfh proteins depends on the nature of divalent ions (Mg(2+) or Mn(2+)) present in the reaction

150 teins 8 (K8) and 18 (K18) in the presence of divalent ions (Mg(2+)).

151 ther globally or locally, in the presence of divalent ions, might constitute a mechanism for regulati

152 erence between AS-1 and AS-2.2) Although the divalent ion Mn(2+) enhances the ligand binding function

153 In the absence of divalent ions, NCS-1 unfolds and refolds reversibly in a

154 ed by determining the effects of seven other divalent ions on erythrocyte membrane properties.

155 y of reversible binding effects of mono- and divalent ions on the chemical shift properties of the Le

156 re generated by exposing alpha4-integrins to divalent ions or by inside-out activation using a chemok

157 ndent inward rectification in the absence of divalent ions or charged regulators such as spermine, in

158 he general acid does not appear to depend on divalent ions or the identity of the Bronsted base.

159 The presence of divalent ions, particularly calcium, appears to be an im

160 tization of alpha7 and dramatically reducing divalent ion permeability relative to wild-type alpha7.

161 n this study, we demonstrate that calcium, a divalent ion, preferentially increases the activity of c

162 thin the PA channel pore and that H+ and the divalent ions probably act via similar mechanisms to aff

163 We find that divalent ions produce a fiber stiffening effect that com

164 s and find it to be lower in the presence of divalent ions rather than only monovalent ions.

165 ments illustrating the enhanced screening of divalent ions relative to monovalent ions at the same io

166 The concentration and specificity of divalent ions required to induce the fluorescence change

167 alpha 8 beta 1-AP chimera exhibited the same divalent ion requirements for activation and binding spe

168 telomerase was characterized with respect to divalent ion requirements, effect of salt and nonionic d

169 These sequence-dependent, divalent ion-sensitive, and structurally unique solution

170 ion with the lower affinity binding to the A divalent ion site.

171 performed to probe the nature of the various divalent ion sites and any specificity for Mg2+.

172 The pseudoknot binds divalent ions somewhat more tightly than a hairpin but s

173 However, it is also likely that a subset of divalent ions specifically occupies cation binding sites

174 Mono- and divalent ions stabilize the native fold of RNA, whereas

175 Group II divalent ions stabilized the parallel G-tetraplexes, and

176 Surprisingly, divalent ions such as Mg(2+), Ca(2+), and Zn(2+) act as

177 The specificity of this effect for different divalent ions suggests binding sites that are not an EF-

178 No other divalent ions tested could replace calcium.

179 In eag, these transitions are modulated by a divalent ion that binds in the gating pocket.

180 DNA and correct incoming nucleotide, and two divalent ions, the thumb subdomain of pol X undergoes a

181 In the absence of divalent ions, the tRNA core gains flexibility under con

182 atalyze the reaction in the absence of other divalent ions, they significantly modulate the reaction

183 mimicks the reactions of the highly reducing divalent ions Tm(II), Dy(II), and Nd(II), has been explo

184 )(py)(2), and (dpm)Co(II)(py)(2) reveal each divalent ion to be high-spin and pseudotetrahedral in th

185 Binding of the catalytic divalent ion to the ternary DNA polymerase beta/gapped D

186 Necessity of the divalent ions to retain the suspension signified the ele

187 Assuming specific binding of divalent ions to RNA, the Mg2+ dependence of the observe

188 Our data also show that, during unfolding, divalent ions together with LHs induce linker-DNA bendin

189 Solute carrier 11A1 (SLC11A1) is a divalent ion transporter formerly known as the natural r

190 carried out a series of experiments to test divalent ion usage and preferences.

191 e resultant peak potentials of the secondary divalent ion vary with its sample activity to yield an a

192 lular Ca(2+) , suggesting that influx of the divalent ion via more Ca(2+) -permeable normal MT channe

193 to block of outward current by intracellular divalent ions, we find that inward rectification is in f

194 significant level over a control in which no divalent ions were added to the media.

195 emerge from the binding energies of the six divalent ions with amino acids and dipeptides.

196 Delocalized electrostatic interactions of divalent ions with nucleic acids should be very weak in

197 endence emphasizes the strong interaction of divalent ions with the membrane and its effect on the me

198 IK(IR) is also blocked by other divalent ions, with Ba2+ >> Sr2+ > Mg2+ > Mn2+ = Ca2+, a