ライフサイエンスコーパス: division site

1 al peptidoglycan-synthesizing enzymes to the division site.

2 actomyosin ring is assembled de novo at the division site.

3 phosphatase, GFP-PhpP, both localize to the division site.

4 onstriction and remains highly mobile at the division site.

5 t of cell division and the previously formed division site.

6 cs of the assembled FtsZ ring at the midcell division site.

7 s a regulator of cell-wall hydrolysis at the division site.

8 s toward MT plus ends located at or near the division site.

9 athway to inhibit FtsZ ring formation at the division site.

10 additional molecular motors to constrict the division site.

11 n and extracellular matrix remodeling at the division site.

12 f cell wall turnover at the Escherichia coli division site.

13 d synthesis of new peptidoglycan (PG) at the division site.

14 es two mechanisms to concentrate Rho1 at the division site.

15 n to promoting PPB formation at the cortical division site.

16 re found to be specifically recruited to the division site.

17 essential for their later appearance at the division site.

18 ted so that it concentrates precisely at the division site.

19 adjacent to but not within the previous cell division site.

20 DV2 to localize PDV2 (and hence ARC5) to the division site.

21 ity, new buds do in fact form within the old division site.

22 ke protein FtsZ, which forms a Z-ring at the division site.

23 nt role in the choice and positioning of the division site.

24 on plane with their plus ends located at the division site.

25 00 nm) filaments spaced erratically near the division site.

26 in placing the new cell wall at the correct division site.

27 rane but did abolish its localization to the division site.

28 new cell wall and is guided to the forecast division site.

29 t least 10 essential proteins at the nascent division site.

30 ire the Z-ring for recruitment to the future division site.

31 novel action of MAP65-4 at the cortical cell division site.

32 ssfully contacting the "programmed" cortical division site.

33 vely known as the divisome, at the incipient division site.

34 emodeling the peptidoglycan cell wall at the division site.

35 as well as efficient removal of Chs4 at the division site.

36 onsible for selection and recognition of the division site.

37 d by a multiprotein complex assembled at the division site.

38 h determines the ability of MapZ to mark the division site.

39 e E. coli both FtsE and FtsX localize to the division site.

40 otein localizes to a ring at the chloroplast division site.

41 izes into a ring that establishes the future division site.

42 ates cell polarity and localizes to the cell division site.

43 oad band of cortex around the equator as the division site.

44 polar zone from extending beyond the midcell division site.

45 pendency order of proteins localizing to the division site.

46 he preprophase band predicts the future cell division site.

47 an active role in determination of the cell division site.

48 Z2, which colocalize to rings at the plastid division site.

49 in an actin-dependent manner and at the cell division site.

50 al ring, an equatorial ring structure at the division site.

51 patiotemporal formation of the Z ring at the division site.

52 er alignment of the mitotic spindle and cell division site.

53 3 are interdependent for localization to the division site.

54 iding phragmoplast expansion to the cortical division site.

55 hile the LtaS enzyme accumulated at the cell division site.

56 required for AmiB's localization to the cell division site.

57 nge factor Gef3p interacts with Rho3p at the division site.

58 t all three proteins localize sharply to the division site.

59 d on actin filaments for localization to the division site.

60 he recruitment of the late components to the division site.

61 rentially at the pole distal to the previous division site.

62 artmentalize the cytoplasmic membrane at the division site.

63 er to position the nuclei away from the cell division site.

64 localization of Cdv proteins but not FtsZ to division sites.

65 le in defining the position of mitochondrial division sites.

66 del may explain why DivIVA localises at cell division sites.

67 nts together minimizes formation of multiple division sites.

68 y polar, localizes at or near potential cell division sites.

69 t abrogated recruitment of FtsI to potential division sites.

70 ecies, as judged by loss of GFP targeting to division sites.

71 therefore behaving as a permanent beacon of division sites.

72 Given that capping protein arrives at the division site 20 min later than Cdc12p, capping protein

73 s the expected burst of PG hydrolysis at the division site, accompanied by amidase recruitment.

74 served mitotic exit kinase, localizes to the division site after Chs2 and directly phosphorylates Chs

75 p, which recruits IQGAP-related Rng2p to the division site, after which other node components accumul

76 eprophase band and persisted at the cortical division site afterwards.

77 We found that FtsN localizes to potential division sites, albeit poorly, in RG60 grown in LB with

78 s, aggregates of ring proteins remain at the division site and at additional foci in other parts of t

79 suggest that de novo F-actin assembly at the division site and directed transport of F-actin cables a

80 oth de novo-assembled actin filaments at the division site and existing filaments recruited by direct

81 rtex, where the localized Mid1 specifies the division site and induces contractile-ring assembly.

82 th because the mutant can recognize the cell division site and influences the localization of MinD.

83 Thus, FtsW is a late recruit to the division site and is essential for subsequent recruitmen

84 and several other proteins that forms at the division site and is important for cytokinesis.

85 that DivIB only transiently localises to the division site and may mark previous sites of septation.

86 sion requires accurate identification of the division site and placement of the division machinery.

87 However, how myosin II is targeted to the division site and promotes AMR assembly, and how the AMR

88 -like FtsZ division protein localizes to the division site and serves a cytoskeletal role during sept

89 ructure that establishes the location of the division site and serves as a framework for assembly of

90 es into a ring-like structure at the nascent division site and serves as a scaffold for the assembly

91 rich membrane domains are formed at the cell division site and sites of polarized cell growth at cell

92 localization of FtsZ, PDV1, and ARC5 at the division site and that PDV1 and PDV2 together mediate th

93 G1 phase, microtubule capture at the recent division site and the incipient bud is dictated by Bud6p

94 both the positioning of the vegetative cell division site and the polar attachment of the chromosome

95 We found that Bud5 is localized at the cell division site and the presumptive bud site.

96 ZapA and ZauP co-localized at the division site and were each required for efficient divis

97 ation of Rga1 from the immediately preceding division site and, consequently, abnormal bud-site selec

98 DivIVA is specifically targeted to cell division sites and cell poles.

99 al through vesiculation and blebbing at cell-division sites and compensatory shrinkage of the inner m

100 rocess ensures the inactivation of old polar division sites and leaves the cell centre free for the a

101 work showed that this protein is targeted to division sites and retained at the cell poles after divi

102 ed on insertion of new wall material at cell division sites and that this localized growth is depende

103 , AtPAKRP2 became more concentrated near the division site, and additional signal could be detected e

104 ins, reduces peptidoglycan remodeling at the division site, and blocks compartmentalization of the cy

105 and in the placement of the chromosome, the division site, and individual structural and regulatory

106 cytokinesis regulators-Iqg1 and Inn1-at the division site, and results in an abnormal septum.

107 , distribution of type V myosin Myo52 to the division site, and timely recruitment of septum protein

108 mainly promoting the recruitment of PBP1 to division sites, and GpsB facilitating its removal from t

109 hat polymerizes into rings localized to cell-division sites, and the constriction of these rings driv

110 to the mother-bud neck and to the subsequent division site are dependent on its GDP-GTP exchange fact

111 el and the initial level of Cdc42-GTP at the division site are perturbed by noise added in the model.

112 As expected, active Rho1 levels at the division site are reduced in art1 and rgf3 mutant cells.

113 between the inner and outer membranes at the division site, as has been previously proposed.

114 ect proteins made in the same cell to active division sites, as when SpoIIQ is made in the mother cel

115 zes to the bud tip until the M phase, to the division site at cytokinesis, and then to the distal pol

116 uniformly along its length, the future cell division site at mid-cell is newly made and has no known

117 Placement of the division site at midcell in Escherichia coli requires th

118 lusion (NO), which cooperate to position the division site at midcell.

119 the engulfed forespore and to the potential division site at the distal pole.

120 cytokinesis, and prolonged exocytosis at the division site at the end of cytokinesis.

121 , which nucleates microtubules from the cell division site at the end of mitosis, and interphase MTOC

122 ously thought to form a static ring near the division site at the midcell position, actually joins Mi

123 gly, both Chs2 and Chs3 are delivered to the division site at the onset of cytokinesis [6, 20].

124 f B. subtilis FtsZ is recruited to potential division sites at both poles of the cell, but asymmetric

125 h as Escherichia coli, often mark their cell division sites at their cell midpoint so that daughter c

126 lacking DipM also showed OM blebbing at the division site, at cell poles and along the cell body.

127 yosin-II in budding yeast, was mobile at the division site before anaphase and became immobilized sho

128 racterize a novel factor that locates at the division site before FtsZ and guides septum positioning

129 It is recruited to active division sites before septal biogenesis, rotates around

130 l origin regions localize to the future cell division sites, before FtsZ.

131 PBP2 localized to the division site but its recruitment to the forming divisio

132 of aerial hyphae with partially constricted division sites but no clear separation of prespore compa

133 SftA colocalizes with FtsZ at nascent division sites but not with SpoIIIE at sites of chromoso

134 I complex have distinct localizations at the division site, but both are important for membrane expan

135 aromyces pombe, four septins localize to the division site, but deletion of the four genes produces o

136 RanGAP1 persistence at the cortical division site, but not its initial accumulation at the P

137 ER-mitochondria contacts mark mitochondrial division sites, but the molecular basis and functions of

138 , like its paralog AmiC, is recruited to the division site by an N-terminal targeting domain.

139 speculate that SPOR domains localize to the division site by binding preferentially to septal peptid

140 d are thought to target proteins to the cell division site by binding to "denuded" glycan strands tha

141 ations suggest that PBP2 is recruited to the division site by binding to its substrate, which is loca

142 ivating protein, prevents budding within the division site by inhibiting Cdc42 repolarization.

143 n Escherichia coli that are recruited to the division site by interaction with FtsZ.

144 We conclude that Nod1 specifies the division site by localising Gef2 to the mitotic cell mid

145 e actin contractile ring and the future cell division site by positioning cytokinesis factors such as

146 lizes to the immediately preceding and older division sites by interacting with Nba1 and Nis1.

147 ne trafficking to and membrane fusion at the division site cause the physical separation of the daugh

148 proteins assemble into a ring at the plastid division site, chloroplasts in the arc6 mutant contain n

149 ated with DamX: targeting the protein to the division site, conferring full resistance to the bile sa

150 ana arc5 mutants, chloroplasts arrest during division site constriction.

151 n pathogen lacking the canonical systems for division site control (nucleoid occlusion and the Min-sy

152 deposits the new cell wall, to the cortical division site delineated by the PPB.

153 For3 localization at the division site depended on the F-BAR protein Cdc15, and f

154 log of the ATPase MinD, which serves in cell-division site determination.

155 ations have validated some early concepts of division site determination.

156 , suggesting that minE and cdv3 both mediate division-site determination in cyanobacteria.

157 ion was blocked by altered expression of the division site-determining factor AtMinD.

158 hough localization of Rax1p and Rax2p to the division site did not appear to depend on Bud9p, normal

159 ombe, an equatorial MTOC (eMTOC) at the cell division site disassembles after cytokinesis, and multip

160 ates induced to insert outside the predicted division site do not elicit an AIR9 torus, suggesting th

161 t the cell tips during interphase and at the division site during cell division.

162 on of the stable crescentin structure at the division site during cytokinesis.

163 guides the phragmoplast towards the cortical division site during cytokinesis.

164 PPB and remains associated with the cortical division site during mitosis and cytokinesis, requiring

165 riments suggest that CtpA accumulates at the division site during the last quarter of the cell cycle.

166 FtsK localizes at nascent division sites during every cell cycle and stimulates ch

167 lization at spindle pole bodies and the cell division site, E3 ligase activity, and mitotic checkpoin

168 FtsA recruits a small amount of FtsN to the division site earlier than previously recognized.

169 sequentially as a multimeric complex at the division site: first FtsZ, then FtsA and ZipA independen

170 rectly bind FtsZ polymers at the prospective division site, followed by the sequential addition of Ft

171 yet the mechanisms for recruiting Rho to the division site for cytokinesis remain poorly understood.

172 whether this involves ring formation at the division site has not been determined previously.

173 we observed (i) initial establishment of the division site, (ii) recruitment of early FtsZ-binding pr

174 data show that Gps1 is targeted to the cell division site in a biphasic manner, via Gin4 and Nba1, t

175 hich other node components accumulate at the division site in a characteristic sequence [3-6].

176 iae, the F-BAR protein Hof1 localizes to the division site in a complex pattern during the cell cycle

177 is an effector, UgtP, which localizes to the division site in a nutrient-dependent manner and inhibit

178 f the septin proteins, which localize to the division site in all animal and fungal cells but are ess

179 DipM localized to the division site in an FtsZ-dependent manner via its PG-bin

180 that ring proteins need not assemble at the division site in an invariant order.

181 tubules of the mitotic apparatus specify the division site in animal cells, we show here that the nuc

182 kbone synthesis proceeds in S. aureus at the division site in co-ordination with cell division, while

183 icating that FtsL and YgbQ colocalize to the division site in E. coli.

184 tem responsible for correct placement of the division site in Escherichia coli cells.

185 Placement of the division site in Escherichia coli is determined in part

186 Selection of the division site in Escherichia coli is regulated by the mi

187 d to play a pivotal role in selection of the division site in eubacteria and chloroplasts.

188 hat plays a crucial role in selection of the division site in eubacteria, chloroplasts, and probably

189 d phosphorylation precociously appear at the division site in filament-like structures and display in

190 conformation, inhibits Cdc15 assembly at the division site in interphase, and precludes interaction o

191 demonstrate that FtsZ, which marks the cell division site in many bacteria, can be targeted to the m

192 system regulates the positioning of the cell division site in many bacteria.

193 tubule-binding proteins that localize to the division site in plants.

194 ture establishes the location of the nascent division site in prokaryotes.

195 spatial distribution by accumulating at the division site in response to external osmotic upshifts.

196 Proper placement of the cell division site in some rod-shaped bacteria requires two d

197 However, FtsQ is found at the cell division site in the absence of FtsL and FtsB.

198 FtsQ2 and FtsQ65 localize to the division site in the presence or absence of endogenous F

199 DV1 localized to a discontinuous ring at the division site in wild-type plants.

200 it follows that FtsN initially localizes to division sites in a SPOR-independent manner.

201 at FtsQ, FtsL and FtsI localize to potential division sites in filamentous cells depleted of FtsN, bu

202 m septal peptidoglycan (SP) rings, appear at division sites in isolated sacculi.

203 n minimal medium or sucrose-free R2YE, where division sites in many aerial hyphae had finished constr

204 The MinCDE proteins help to select cell division sites in normal cylindrical Escherichia coli by

205 to localize to division sites, localized to division sites in the absence of FtsK, indicating that F

206 forms membrane domains at the cell pole and division sites in wild-type cells, the mutant cells stil

207 esize new wall material specifically at cell division sites, in the form of a flat circular plate tha

208 , IcsA localizes to poles and potential cell division sites independent of the cell division protein

209 llows us to target a protein directly to the division site independently of other cell division prote

210 o report that AmiB and EnvC are recruited to division sites independently of one another.

211 EnvC accumulates at division sites independently of this essential division

212 ted chromosome masses at the normal mid-cell division site inhibits Z-ring formation and that the SOS

213 Concentration of Rho proteins at the division site is a general feature of cytokinesis, yet t

214 Accurate placement of the bacterial division site is a prerequisite for the generation of tw

215 sembly of the FtsZ ring (Z ring) at the cell division site is negatively regulated by the nucleoid in

216 nesis and that de novo actin assembly at the division site is predominant for contractile ring format

217 osed to guide the cell plate to the cortical division site is unknown.

218 ed and the order of appearance at the future division site is well organized.

219 The protein localized to the division site late in mitosis, where it formed a ring th

220 as fully rescued by introduction of cortical division site localized TANGLED1-YFP.

221 which normally depend on FtsK to localize to division sites, localized to division sites in the absen

222 myosins in cytokinesis, and in how the cell division site may be positioned by the nucleus.

223 pression in Arabidopsis seedlings results in division site misplacement giving rise to multiple const

224 At the division site, most bacteria assemble filaments of the t

225 vision protein leads to the absence from the division site of "downstream" proteins in the pathway.

226 one and VirE2 secretion substrate complex to division sites of E. coli and A. tumefaciens.

227 arget heterologous protein complexes to cell division sites of E. coli and Agrobacterium tumefaciens.

228 in DivIVA accumulates at cell poles and cell division sites of Gram-positive bacteria.

229 bserved at the distal pole as well as at the division site on both mother and daughter cells; localiz

230 ughter cells as well as near previously used division sites on mother cells.

231 e generated inside the cell to constrict the division site or to haul the rear of the cell forward, r

232 dynamic changes that play important roles in division site placement and possibly other aspects of th

233 ional principles, such as spatial control of division site placement by intracellular protein gradien

234 Division site placement in Escherichia coli involves int

235 focus on the MinCD/DivIVA system regulating division site placement in the rod-shaped bacterium Baci

236 Our data suggest that division site placement is determined by cortical positi

237 In Escherichia coli, division site placement is regulated by the dynamic beha

238 suggest that the topological specificity of division site placement may not involve a localized acti

239 f the ability to support a normal pattern of division site placement.

240 idopsis thaliana, we show that ARC3 mediates division-site placement by inhibiting Z-ring assembly, a

241 Thus, proper control of B. abortus division site polarity is necessary for survival in the

242 Here, we explore the plasticity of division site positioning in fission yeasts Schizosaccha

243 Division site positioning is critical for both symmetric

244 The division-site positioning defects of gef2Delta plo1-ts18

245 with various cytokinesis mutants involved in division-site positioning, suggesting a role of Nod1 in

246 ive bacteria that localizes at the poles and division sites, presumably through direct sensing of mem

247 s specific proteins to populate the cortical division site prior to disassembly of the preprophase ba

248 elope, PBP5 was also concentrated at nascent division sites prior to visible constriction.

249 DivIVA and MinD recruit MinC to division sites, rather than mediating the stable polar l

250 The division-site regulators ACCUMULATION AND REPLICATION OF

251 at Axl1 localizes to the mother-bud neck and division site remnants of haploids.

252 P) that was fused to an LZ domain to E. coli division sites, resulting in fluorescence patterns ident

253 nism is different from those employed by the division site selection antagonists MinC and SlmA.

254 The Min proteins that govern division site selection in Escherichia coli may be the f

255 Division site selection in Escherichia coli requires tha

256 Division site selection in rod-shaped bacteria depends o

257 DNA replication, chromosome segregation, and division site selection in the pneumococcus, providing a

258 ia and may be an important component of cell division site selection in these organisms.

259 d-shaped bacteria such as Bacillus subtilis, division site selection is mediated by MinC and MinD, wh

260 In Escherichia coli, division site selection is regulated in part by the Min-

261 or of competence ComN, and the regulators of division site selection MinD and MinJ.

262 s not require MinC, the main effector of the division site selection system.

263 system results in defects in cell growth and division site selection, and a specific viability defici

264 S/ParB/ParA system directly affects not only division site selection, but also cell growth.

265 inD oscillation behavior, and therefore cell division site selection, may be regulated by membrane ph

266 stion remains as to what drives pneumococcal division site selection.

267 as identified and implicated in pneumococcal division site selection.

268 er than normal levels of FtsZ or lacking the division-site-selection protein DivIVA.

269 traction to the cell pole is mediated by the division-site-selection protein, MinD (which localizes a

270 Tomographic cryoEM images of the cell division site show separate constrictive processes closi

271 is a good understanding of the mechanisms of division site specification in most organisms, and the m

272 ipolar signals and proteins that target cell division site tags to their proper location in the cell.

273 P) Rga1 establishes an exclusion zone at the division site that blocks subsequent polarization within

274 ein FtsZ into a ring (Z-ring) at the nascent division site that serves as a foundation for assembly o

275 However, instead of polarizing at the division site, the new polarity axis is directed next to

276 ses with the plasma membrane at the cortical division site, the site of the former PPB.

277 ocalize with PPBs and remain at the cortical division site through metaphase.

278 ions, lead instead to rapid lysis at nascent division sites through a process that requires the compl

279 cruitment of many additional proteins to the division site to form the mature divisome machine.

280 metric cell division: the relocation of cell division sites to the cell poles and the asymmetric part

281 The arrival of FtsN at the division site triggers synthesis of septal peptidoglycan

282 mperatures but are unable to localize to the division site unless wild-type FtsQ is depleted, suggest

283 nd the protein Inn1 are all delivered to the division site upon mitotic exit even when the AMR is abs

284 ks phragmoplast microtubules to the cortical division site via actin filaments.

285 rgeting and activation of Rho1 (RhoA) at the division site via Rho1 guanine nucleotide exchange facto

286 s cerevisiae, and that Myo1 localizes to the division site via two distinct targeting signals in its

287 tion of DnaK-GFP to poles and potential cell division sites was dependent on elevated growth temperat

288 ther and daughter cells; localization to the division sites was persistent through multiple cell cycl

289 ments indicate that EnvC is recruited to the division site well before its cognate amidase AmiB.

290 t growth, morphology and positioning of cell division sites were abnormal relative to Min+ cells.

291 ore frequent Cdc42 repolarization within the division site when the first temporal step in G1 is assu

292 aments under the cytoplasmic membrane at the division site where, together with other proteins it rec

293 ane protein that localizes to the polar cell division sites where it causes FtsZ to relocate from mid

294 Tol-Pal is recruited to cell division sites where it is involved in maintaining the i

295 dozen (known) proteins that localize to the division site, where they direct assembly of the divisio

296 main mediates dynamic localization to active division sites, whereas the assembly of a stable focus a

297 ition of LysM-type murein hydrolases to cell division sites, which was associated with defects in cel

298 Hof1 colocalizes at the cell division site with the septin complex and, as mitotic ex

299 is necessary for the proper placement of the division site within the cell.

300 1 is involved in preventing rebudding at old division sites, yet how these proteins and Rga1 might fu