ライフサイエンスコーパス: dot blot

1 ncoprotein expression using western blot and dot blot.

2 Enhanced expression was verified by RNA dot blot.

3 LL-37 expression was determined by immune dot blot.

4 and enables it to bind PtdIns(3,5)P(2) on a dot-blot.

5 cloned and subsequently confirmed by reverse dot blotting.

6 sica napus, using northern hybridisation and dot blotting.

7 esponse to AS, a hypothesis confirmed by DNA dot blotting.

8 and developed using the same protocol as in dot-blotting.

9 Whereas dot-blotting allows the use of very low quantities of sa

10 Western and protein dot blot analyses and indirect immunofluorescence were u

11 Northern and dot blot analyses revealed that myosin XV is expressed i

12 Ligand and dot blot analyses revealed zinc-dependent binding of bio

13 The results of Northern and dot blot analyses show that ALF is expressed almost excl

14 Enzyme-linked immunosorbent assay and dot blot analyses showed MBP bound to the surface of the

15 Abs showed reactivity to CNF1 by Western and dot blot analyses.

16 eptor protein was detected using western and dot blot analyses.

17 t of these clones bound mAb TL4 in ELISA and dot blot analyses.

18 Northern and dot-blot analyses showed a large reduction in LOX-2 mRNA

19 at was further supported by Western-blot and dot-blot analyses, as well as by inhibition of binding b

20 Utilizing Northern dot blot analysis and a quantitative reverse transcripti

21 Northern dot blot analysis and comparative densitometry confirmed

22 in the transfected cells was demonstrated by dot blot analysis and ELISA.

23 n of these genes by 2,4-DNP was confirmed by dot blot analysis and two of them were confirmed to be i

24 Dot blot analysis demonstrated that nonfibrillar Abeta o

25 Northern dot blot analysis demonstrated that Tctex1/Tctex2 family

26 Dot blot analysis established that packaging efficiency

27 Immuno-dot blot analysis identified the cis-syn cyclobutane pyr

28 cids, respectively, were measured by Western dot blot analysis immediately after exposure to light.

29 Food allergens were measured by dot blot analysis in mattress dust from 143 homes in Osl

30 Southern and dot blot analysis indicate that the tigA gene is present

31 RNA dot blot analysis of 100 cases of primary tumors suggest

32 DNA dot blot analysis of 106 V. cholerae strains showed the

33 entrations of glandular protein when Western dot blot analysis of collagens I and III and laminin, re

34 atoid papulosis cases were then subjected to dot blot analysis of genomic DNA using a full-length HTL

35 RNA dot blot analysis of human multiple tissue expression ar

36 Dot blot analysis of mRNA from 50 human tissues indicate

37 Data from dot blot analysis of PCR products were consistent with t

38 Dot blot analysis of RNA preparations confirmed the low-

39 According to fluorescence polarization and dot blot analysis of synthetic DnaK fragments and labele

40 Western dot blot analysis showed a 3.4-fold increase in protein

41 Dot blot analysis showed that the "raft" associated gang

42 Dot blot analysis using a panel of synthesized oligosacc

43 Dot blot analysis with 11 DNA sequences from this PAI de

44 d difference in intensity were rescreened by dot blot analysis with the same probes and with mixed cD

45 By dot blot analysis, 4-HNE- and 4-HHE-modified proteins we

46 Using bacterial lysates in dot blot analysis, a panel of sera from normal individua

47 s, we employed immunofluorescence labelling, dot blot analysis, Fourier transform Raman spectroscopy,

48 after IL-8 exposure, was observed using RNA dot blot analysis.

49 he prototype subtype P1.14 strain, S3446, by dot blot analysis.

50 growing bacteria was investigated by immuno-dot blot analysis.

51 measure the relative telomere DNA content by dot blot analysis.

52 Dot-blot analysis and RT-PCR revealed that human PKD1L1

53 Human RNA dot-blot analysis detected this mRNA in all tissues exam

54 Dot-blot analysis of p38delta mRNA in 50 human tissues r

55 Dot-blot analysis of the isolated clones verified differ

56 t had accumulated in cultured cells, whereas dot-blot analysis revealed binding to both A2E and A2E-r

57 In addition, dot-blot analysis showed approximately 5-10% of C5 and C

58 its direct association to the enzyme because dot-blot analysis using antibody to glutathione S-transf

59 m WHV DNA was not detectable by conventional dot-blot analysis, hepatic WHV-DNA replicative intermedi

60 By dot-blot analysis, we estimate there are 150,000, 4,000,

61 Using library screening and dot-blot analysis, we estimate there are 25,000 copies o

62 Modified retinal proteins were quantified by dot-blot analysis.

63 DNA dot blot and biochemical assay of viral DNA polymerase a

64 Stromelysin mRNA levels were evaluated by dot blot and by reverse transcription, followed by polym

65 Dot blot and ELISA assays demonstrate the inhibition of

66 eningitidis were examined for MBL binding by dot blot and ELISA.

67 ing to An. gambiae BBMV and bound Cry11Ba on dot blot and microtiter plate binding assays with a calc

68 Dot blot and Northern analyses of MCPS MAbs revealed tha

69 ir expression patterns were confirmed by RNA dot blot and RT-PCR analyses.

70 transcription was confirmed by quantitative dot blot and Western blot analyses.

71 Dot blot and western blot assays are used to assess anti

72 IBM autoantigen, which was then confirmed in dot blot and Western blot assays using recombinant cN1A

73 ibody were determined in infected monkeys by dot blot and Western blot assays, respectively.

74 and plasmin protein levels were measured by dot blot and Western blot, respectively, while plasmin a

75 Viremia, measured by dot blot and/or PCR, and SPV-specific antibodies were de

76 Selected cosmids were spotted on dot blots and assigned to bins defined by YACs.

77 CMV-specific DNA dot blots and biochemical enzyme assays indicated that,

78 ometric analysis of semiquantitative Western dot blots and by immunohistochemistry, using 4-HNE- and

79 Both dot blots and immunocytochemistry show that allurin is s

80 expressed AgALP1(t) bound [(125)I]Cry11Ba on dot blots and reduced the level of binding of [(125)I]Cr

81 DU-145 cells was demonstrated by Western and dot blotting and flow cytometry with monoclonal antibodi

82 actin, or NC1 domain of type VII collagen by dot blotting and western blotting.

83 raised to the PorB peptides and were used in dot-blot and ELISA-based absorption studies with viable

84 asured catalase protein expression by immune dot-blotting and catalase mRNA by a real-time polymerase

85 Two other assays, dot-blotting and immunoblotting, detect KS in complex mi

86 pimonidazole-protein adduct quantification (dot blot) and as a surrogate for transcriptional activat

87 aLP bound [(125)I]Cry1A toxins under native (dot blot) and denaturing (ligand blot) conditions.

88 ymerase chain reaction (PCR)/ Southern blot, dot blot, and Southern blot analyses.

89 sed transcriptional reporter constructs, RNA dot blots, and Western blots to examine the expression o

90 irst shown using fluorescence microscopy and dot blotting, and further demonstrated using flow cytome

91 Northern blot, dot-blot, and reverse transcription-coupled PCR analyses

92 have been developed and validated by ELISA, dot-blot, and Western blot analysis.

93 biotinylated substrate in conjunction with a dot blot apparatus to eliminate the use of radioactive s

94 Peptides are assayed in a 96-well dot blot apparatus using membranes that enable partition

95 We describe use of a convenient "dot-blot" approach to screen 10 different PH domains for

96 y breast tumors, we screened a breast cancer dot blot array of normalized cDNA from 50 breast tumors

97 Western blotting (Dot Blot) as an immunological method confirmed biologica

98 the constructs were tested in a solid-phase dot blot assay followed by confirmatory quantitative che

99 The sensitivity and specificity of a dot blot assay for detection of human antibodies with th

100 ave developed an amplification-based reverse dot blot assay for the detection of specific sites of in

101 An immuno-dot blot assay that could detect LP at levels as low as

102 The detection of hTSH in a dot blot assay with radiolabeled T-15 RNA was demonstrat

103 se 34 IFA-positive sera were positive by the dot blot assay with rP30, distinguishing them from IFA-n

104 xigenin (DIG)-labeled human lactoferrin in a dot blot assay.

105 er typed for colonization factor antigens by dot blot assay.

106 12 colonization factor antigens (CFAs) by a dot blot assay.

107 qualitatively ranked by a new Dynabead-based dot blot assay.

108 which is specific for sialylated species, by dot blot assay; this reactivity was also reversed by neu

109 Here we describe an efficient dot-blot assay for high-throughput screening of two enzy

110 Finally, we developed a dot-blot assay for the glycan-specific IgG antibody that

111 Dot-blot assay reveals that the TrioN-PH domain does not

112 We modified the traditional dot-blot assay to serve as an identity test for monoclon

113 Using the MAb dot-blot assay, 5 of 16 commercial DNA products obtained

114 as quantified by periodic acid-Schiff's base dot-blot assay, using purified pig gastric mucin as a st

115 omatography and a recently developed polymer dot-blot assay.

116 before ISH using a RNA probe quantification (dot blot) assay.

117 RNA dot blot assays confirmed that expression of the acidic

118 ction by EIS was further supported by immuno dot blot assays for oligomeric and fibrillar components.

119 report we used yeast two-hybrid and in vitro dot blot assays to further examine the requirements for

120 n immunoblotting, the results of Western and dot blot assays with rP30 matched 100% with the IFA test

121 ion sequencing technology), and quantitative dot blot assays.

122 luble heparin specifically in inhibition and dot blot assays.

123 Collectively, the EB absorption and dot-blot assays established that the immunoreactive PorB

124 ant corroborated the results of quantitative dot-blot assays of ETA.

125 em, monoclonal antibodies (MAbs), ELISA, and dot-blot assays were developed for the specific identifi

126 nditions, as assayed by quantitative Western dot-blot assays.

127 A novel dot blot-based assay system for the detection of IgE aga

128 emonstrate a simple and robust drug-assisted dot blot bioassay for endotoxin detection that can be us

129 A dot blot capsular type was assigned to 99% (303 of 306)

130 colonizing isolates were used to compare our dot blot capsular typing (DBCT) identification method wi

131 .2 nm for TEM images), and most importantly, dot blotting confirmed immunological activity of the col

132 A cDNA dot blot consisting of differentially expressed genes re

133 CR/Southern blotting, Northern blotting, and dot blotting demonstrated the presence of the platelet-t

134 The new dot blot detection system resolved individual IgE sensit

135 matography combined with rapid and sensitive dot-blot detection.

136 indings suggest that the developed MAb based dot blot ELISA is a simple, rapid performed in less than

137 ere included for the evaluation of MAb-based dot blot ELISA.

138 ophysical assays including amyloid kinetics, dot blot, ELISA, and TEM show that 5 effectively inhibit

139 od relative to that of a previously reported dot blot format.

140 enous beading, whereas those with 4-quadrant dot-blot hemorrhages (4Q DBH) had 3.84 higher HR of deve

141 Dot/blot hemorrhages, cotton-wool spots, and small nonpe

142 nce of B. pertussis PCR products detected by dot blot hybridization alone.

143 thods to determine the PlA genotype: reverse dot blot hybridization and allele-specific restriction d

144 representative isolates was also analyzed by dot blot hybridization and amplification with the polyme

145 HPV was detected by dot blot hybridization and genotyped by the liquid bead

146 Dot blot hybridization and PCR amplification of 14 Ara(+

147 ch 11 cDNA clones were found by differential dot blot hybridization and virtual Northern analysis to

148 nd FAP59/64 primer systems and identified by dot blot hybridization and/or direct sequencing.

149 In addition, dot blot hybridization determined that 3 of 35 strains t

150 investigated by in situ hybridization and by dot blot hybridization for opticin.

151 n reaction (PCR), real time PCR (RT-PCR) and dot blot hybridization have also been proposed for patho

152 Parvovirus B19 (B19) DNA was detected by dot blot hybridization in sera from 5 (17%) of 30 human

153 However, genotype determination by dot blot hybridization is laborious and requires at leas

154 Dot blot hybridization of DNA samples from the F1 offspr

155 Dot blot hybridization revealed that the C. jejuni genes

156 Dot blot hybridization showed that the three probes disp

157 her agarose gel electrophoresis (PCR-gel) or dot blot hybridization with (32)P-labeled oligonucleotid

158 We describe a typing method that uses DNA dot blot hybridization with probes generated by PCR from

159 by the subtraction were screened, using DNA dot blot hybridization, against a collection of 88 uropa

160 ganisms were also distinguishable by DNA-DNA dot blot hybridization, by sequences of two hypervariabl

161 he IS481 PCR, with either electrophoresis or dot blot hybridization, is a sensitive assay; however, a

162 Using PCR and dot blot hybridization, we determined that the his opero

163 c lesions was enhanced by a chemiluminescent dot blot hybridization, which produced a sensitivity of

164 rofile of putative adhesin-encoding genes by dot blot hybridization.

165 1 L1 consensus primer PCR method followed by dot blot hybridization.

166 s media and throat strains was determined by dot blot hybridization.

167 by MY09/MY11 L1 primer PCR and type-specific dot blot hybridization.

168 Thirty specimens were positive by PCR with dot blot hybridization; no negative control specimens sh

169 er RNA (mRNA) were evaluated by Northern and dot-blot hybridization analyses.

170 ium salinarum GRB chromosome was analyzed by dot-blot hybridization of an ordered cosmid library usin

171 Traditional 'dot-blot hybridization' or PCR-based assays for identify

172 e amplification by Southern hybridization or dot-blot hybridization, and for gene expression by North

173 cts were detected by gel electrophoresis and dot-blot hybridization.

174 tative virulence genes using high-throughput dot-blot hybridization.

175 -myc, c-erb, and c-src was quantitated by a "dot-blot" hybridization assay.

176 On the basis of dot-blot hybridizations, seven repetitive DNA motifs acc

177 similar with purified mRNA and total RNA in dot blot hydridizations for cultures grown with or witho

178 wer detection limit and analysis time than a dot blot immunoassay (8.88x10(6) cfu mL(-1) for LOD and

179 or BKV, none of the samples were found by a dot blot immunoassay to have antibodies which cross-reac

180 evaluated by Western immunoblot analysis and dot blot immunoassay.

181 and the fractions collected were screened by dot-blot immunoassay by means of monoclonal antibodies g

182 zation-blocking activity were carried out by dot-blot immunoassays and showed that neuroprotective ex

183 Immunoassays such as dot blot, immunoblot, and enzyme-linked immunosorbent as

184 Studies by using dot blotting, immunoblotting, circular dichroism spectro

185 Microwave-assisted dot blotting is suggested as an effective way of facilit

186 Dot-blot is a versatile and simple analysis to perform.

187 es and an epidemic genetic background by DNA dot blotting, IS1004 fingerprinting, and restriction fra

188 assay, Western blotting, capture assays, and dot blots, less than 25% are capable of neutralizing let

189 nd ImageQuant software demonstrated that the dot blot method can be used to rapidly analyze a large n

190 We developed an immunochemical dot blot method for quantitation of protein carbonylatio

191 eminal ganglia of rabbits to demonstrate the dot blot method of PCR product analysis.

192 The dot blotting method further verifies a positive reaction

193 re determined using a semiautomated, reverse dot-blot method.

194 by means of a noninvasive PCR-based reverse-dot-blot method.

195 We studied, by dot-blot, NACP levels in the frontal cortex of AD cases

196 screening and further confirmed in vitro by dot blots, native electrophoresis, and fluorescence stud

197 ucrose density centrifugation and subsequent dot-blot Northern analysis revealed that antioxidants re

198 outine surveillance samples were analyzed by dot-blot Northern hybridization to detect RotaTeq vaccin

199 the length of the PAI and were hybridized to dot blots of genomic DNA isolated from clinical isolates

200 Moreover, immunostainings and dot blots of optic nerve and myelin showed that expressi

201 VL-horseradish peroxidase conjugate bound to dot blots of VSP or SVL, and binding was inhibited by po

202 Screening of an RNA dot-blot panel confirmed that Kell is primarily expresse

203 roliters of undiluted CSF, sample digestion, dot blotting, Perls' histochemistry, 3,3'-diaminobenzidi

204 presence of ETEC DNA of positive samples by dot blot procedure.

205 We developed a reverse dot blot (RDB) to screen for multiple serotypes in these

206 Densitometric analysis of dot blot reactions showed a positive correlation between

207 d using pools of BAC DNA in combination with dot-blots reveals the locus specificity of individual BA

208 ), rapid amplification of cDNA ends PCR, RNA dot blotting, RNase protection assay, and Northern blot

209 In dot-blotting, samples were directly blotted onto nitroce

210 TLV-I in any PCR-positive case using genomic dot blotting (sensitivity > 10(-2)), and (iii) negative

211 A human tissue RNA dot blot showed that RGS5 message is highest in aorta, f

212 Northern and dot blotting showed that SAMP14 expression was testis-sp

213 A)-specific peptide were characterized using dot blot, sodium dodecyl sulphate-polyacrylamide gel ele

214 Dot blot studies further validated the BAX-IGFBP-3 bindi

215 ay (RPA) assays use micro-scale, cell lysate dot blots that are printed to a substrate, followed by q

216 With dot blots, the time savings was accompanied by a decreas

217 immunoprecipitation, immunofluorescence, and dot blots to examine the effects of TNF-alpha.

218 cted candidate EST clones were tested by RNA dot blots to examine tissue specificity and by Northern

219 lfate-polyacrylamide gel electrophoresis and dot blot, using both monoclonal anti-human Epo antibody

220 ral monolayer compartments were evaluated by dot-blot, using the sera of milk allergic children (N=5)

221 Using Northern blots and RNA dot blots, we have now found that XAGE-1 is highly expre

222 Using yeast two-hybrid analysis and in vitro dot-blots, we show that MLK2 and MLK3 interact with the

223 Dot blots were used as a quantitative assessment of cort

224 A2 (PLA2) activity, as determined by native dot blot Western and PLA2 assays, respectively.

225 halis heterologous isolates were screened by dot blot with a panel of four additional MAbs specific f

226 with results from a previous analysis using dot blots with a radiolabeled nested generic probe mix a

227 n (RT-PCR), colony hybridization and reverse dot blot yielded three distinct probes.