ライフサイエンスコーパス: double mutant

1 fect that is synergistically enhanced in the double mutant.

2 Y181C and K103N mutants and the Y181C/K103N double mutant.

3 ing an EC50 of 11 nM against the Y181C/K103N double mutant.

4 based on phenotypes conferred by a sim smr1 double mutant.

5 amples scored as wild-type, D538G, Y537S, or double mutant.

6 relative to infection by any other single or double mutant.

7 cumulation is strongly reduced in a cda nsh1 double mutant.

8 olonged longevity, which was enhanced in the double mutant.

9 escued the conditional male sterility in the double mutant.

10 these effects are exacerbated in a mneP mneS double mutant.

11 defect was more severe in the FgSch9/FgHog1 double mutant.

12 wild type, was observed with the T352A/M362T double mutant.

13 utant and was also increased in an opaR aphA double mutant.

14 0 value of 1 nM against the EGFR L858R/T790M double mutant.

15 n ipk1Delta kcs1Delta or ddp1Delta kcs1Delta double mutants.

16 accumulate in the ciliary shaft of ome;crb3a double mutants.

17 rformed RNA sequencing (RNA-Seq) on ant ail6 double mutants.

18 S6 single alanine mutants and corresponding double mutants.

19 aCRY and created the chr1 chr2 and uvr8 phot double mutants.

20 ntation fails to rescue Cox2 levels of these double mutants.

21 in genes, generating all possible single and double mutants.

22 tituted with Cys to generate single and nine double mutants.

23 e differentially regulated in serk1-3serk3-2 double mutants.

24 5/6 and Eso1 dysfunction are cosuppressed in double mutants.

25 e for cell death attenuation in cat2-2 shr-6 double mutants.

26 P2-KO background to generate APPDeltaCT15-DM double mutants.

27 mes in the sensory axons of spectrin and tau double mutants.

28 Roots of the double mutant aar1-1 tir1-1 also showed enhanced resista

29 Here we show that gE(-) US9(-) double mutants accumulate large quantities of unenvelope

30 on, and when coexpressed with KIN10 the WRI1 double mutant accumulated to 2- to 3-fold higher levels

31 sine-deficient medium, but an mdh3/gpd1Delta double mutant accumulates saccharopine and displays lysi

32 ramatic synergistic effect was found for the double mutant across all parameters.

33 Of the 28 mTOR double mutants, activating mutations could be divided in

34 The DeltainvDeltayadA double mutant adhered least to cells and so was not sign

35 In yeast, the pol3-01,L612M double mutant allele, which causes defects in DNA polyme

36 Similar experiments with double mutants alpha-NH2Y730/C439A and alpha-NH2Y731/Y73

37 The double mutant also showed abnormal development of primar

38 s are required for survival of both types of double-mutant AML.

39 s 9 dimethylation (H3K9me2) levels at AtMu1c Double mutant analyses with epigenetic silencing mutants

40 mbining single site-directed mutagenesis and double mutant analyses, we conduct a detailed analysis o

41 ts showed subtly elevated levels of lesions, double mutant analysis disagreed with a simple epistatic

42 Double mutant analysis revealed that SmD3b is also invol

43 Double mutant analysis revealed that the nlp7-1 phenotyp

44 Double-mutant analysis indicates that early flowering is

45 Through single- and double-mutant analysis to study the mitotic cohesion pro

46 s impaired the infectivity of both the F-MLV double mutant and the wild-type F-MLV strain, suggesting

47 restored to wild-type levels in a luxO opaR double mutant and was also increased in an opaR aphA dou

48 hannel-like protein isoforms 1/2 (Tmc1:Tmc2) double mutants, and during perinatal development.

49 Analysis of Fat4;Yap and Fat4;Taz double mutants, and expression of their transcriptional

50 d the structure of vegetative SAMs in ltm sp double mutants, and late flowering was partially suppres

51 n complement the severe phenotype of brx ops double mutants, and the most active variants eventually

52 In these Apc-Grp78 double mutant animals, stem cells were rapidly lost and

53 ding of amyloid-beta pathology in transgenic double-mutant APPSwePSEN1dE9 mice.

54 PP transgenic mice (Tg2576 line) and created double mutant (APPXDrp1+/-) mice.

55 hable from wild-type plants, while fps1/fps2 double mutants are embryo lethal.

56 regulation of nodulation and Ljein2a Ljein2b double mutants are hypernodulating and hyperinfected.

57 ntly isolated Arabidopsis thaliana amy3 bam1 double mutants are hypersensitive to osmotic stress, sho

58 ystematic genetic interaction screen whereby double-mutants are created from a large library of singl

59 also performed SGA screen with the amk2 gsk3 double mutant as a query.

60 he Deltalpp single or the Deltalpp DeltamsbB double mutant augmented the attenuation to provide 90 to

61 and betaca4 However, the growth rate of the double mutant, betaca2betaca4, was reduced significantly

62 Only the cox/cyd double mutant, but not single mutants, demonstrated a hi

63 higher binding affinity for the recombinant double mutant by surface plasmon resonance and to the mu

64 c3r with wild-type human (MC3R(hWT/hWT)) and double-mutant (C17A+G241A) human (MC3R(hDM/hDM)) MC3R, t

65 Double mutant cells are able to grow under excess illumi

66 Importantly, in slx4Delta sae2Delta double mutant cells these phenotypes are exacerbated, ca

67 ed in the OXI1 null mutant (oxi1) and in the double mutant ch1*oxi1 compared with the wild type and t

68 Seeds for this double-mutant collection are publicly available through

69 rse biological processes can now screen this double-mutant collection under a wide range of growth co

70 for evidence of genetic interactions in this double-mutant collection.

71 in single mutants and, therefore, requiring double mutant combinations for functional investigation.

72 Different double mutant combinations showed that a low amount of C

73 erturbations to the overall structure of the double mutant compared to the wild-type protein but iden

74 s NRT2.1 and NRT2.4 increased in the bt1/bt2 double mutant compared to wild-type plants, with a conco

75 nes that are differentially expressed in the double mutant compared with the wild type.

76 arget genes was strongly reduced in eol1 clf double mutants compared with clf single mutants.

77 ptotic cell density in Foxg1(-/-);Wnt8b(-/-) double mutants compared with the Foxg1(-/-) single mutan

78 he wild type, while those of the ptst2 ptst3 double mutant contained even fewer granules than ptst2 T

79 Finally, we show that the double mutant contains 30% fewer chloroplasts per cell.

80 find that myp2Delta and myp2Delta myo51Delta double mutants contract actomyosin rings at approximatel

81 n-1 and Phe-350 in rASIC1a was proposed from double mutant cycle analysis.

82 eltaDeltaGo values >4.2 kJ/mol obtained from double mutant cycle analysis.

83 Double-mutant cycle analyses revealed strong functional

84 connectivity between non-adjacent residues, double-mutant cycle analysis was conducted with 22 kines

85 n neighboring alpha-subunits, we performed a double-mutant cycle analysis with Kv4.2 tandem-dimer con

86 no additional structural rearrangements, and double mutant cycles revealed similar contributions from

87 Other strategies involve the use of double mutant cycles, of molecular balances, of dynamic

88 determined experimentally from thermodynamic double mutant cycles.

89 -vis titrations in combination with chemical double-mutant cycles (DMCs) have been used to study the

90 vir-terminated primers, as compared with the double-mutant D67N/K70R.

91 An mbfA bfr double mutant defective in iron export and storage activ

92 Interestingly, the Momsb2 cbp1 double mutant deleted of both mucin genes was blocked in

93 Infection with double-mutant DeltaM36/M45mutRHIM virus reveals a signal

94 ed a negative impact on c-di-GMP levels in a double mutant DeltarsmIE through the control of cfcR, wh

95 A double mutant derived from the VA387_1996 variant contai

96 velopment in accessions, and cyp79b2 cyp79b3 double mutants developed fewer and shorter lateral roots

97 ll for pRb, p107, p130 or any combination of double mutants did not develop melanoma.

98 A insertion mutant erf74 and the erf74;erf75 double mutant displayed higher sensitivity.

99 The L119W/G301F-SbCAD4 double mutant displayed its substrate preference in the

100 tatic to HXK1, as the Glc insensitive2bri1-6 double mutant displayed severe defects in hypocotyl elon

101 ortholog mbl-1 and the ELAVL ortholog exc-7, double mutants displayed a severely shortened lifespan.

102 Many double mutants displayed fitness defects, revealing synt

103 Double mutants disrupting NAC78 and its closest relative

104 e of markedly low tissue levels of iron, the double mutant does not up- and down-regulate iron defici

105 this suppression is rescued by a K113E/E195K double mutant (E/K) restoring the interaction in the opp

106 We also engineered a double mutant, E497K/C566D, that changes the enzyme to a

107 lial adhesions in the most severely affected double mutant embryos ( Irf6(+/-);Tg(KRT14::Spry4)).

108 vating Nodal levels in aplnra/b morphant and double mutant embryos is sufficient to rescue cardiac di

109 anophore lineage is increased in pax7a/pax7b double-mutant embryos and larvae, whereas juvenile and a

110 In Foxc1/2 double-mutant embryos, somitogenesis is severely affecte

111 Lateral roots of the atlazy2,4 double mutant emerged slightly upward, approximately 10

112 man cells, followed by a rigorous single and double-mutant epistasis analysis using CRISPR/Cas9-media

113 Surprisingly double mutant era squa plants display a squa phenotype d

114 Using a genetics approach with the double mutants era1 abi1-1 and era1 ost1, we show that w

115 utant (tapx), the (1)O2-retrograde signaling double mutant (ex1/ex2), and an apoplastic signaling dou

116 Unexpectedly, Eed and Eed/Ezh2 double mutants exhibit delayed superficial cell differen

117 As a result, the tapetum in the double mutant failed to properly deposit the pollen coat

118 The generation of a double mutant Fbln1 and Fgf8 mice (Fbln1(-/-) and Fgf8(-

119 We also employed CRISPR-Cas9 to generate double mutant fish of sp7a;mstnba with high efficiencies

120 aralogous mutant individually as well as the double mutant fish.

121 Double mutants for the Slit receptors Dscam1 and robo1 s

122 e this study, we developed a high-throughput double-mutant generating pipeline using a system for gro

123 on (CRISPR-SGI) approach enabling systematic double-mutant generation.

124 In contrast, the trt1Delta blmDelta double mutant gives rise to survivors as readily as the

125 logy to wild-type granules, but those of the double mutant had an aberrant morphology.

126 In support of this, a sidM lspF double mutant had an intracellular growth defect that wa

127 The Fgskn7 Fgatf1 double mutant had more severe defects in growth, conidia

128 The Deltatrx1 Deltatrx2 double mutant had more severe defects than the Deltatrx2

129 Although the double mutant had virus shedding titers and transmissibi

130 ce with combined mutations in Zeb2 and Edn3 (double mutants) had more severe enteric anomalies and in

131 We show that tan1 air9 double mutants have a synthetic phenotype consisting of

132 The tan1 air9 double mutants have significant defects in division plan

133 dcc and nrp1a double mutants have significantly more DZ misprojections

134 erevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and a

135 substrate specificity are not rescued in the double mutant, implying that functional sequence variati

136 t in the dark and increased in a phytochrome double mutant in the light, indicating that PIFs elevate

137 f flowers to leafy shoots, mimicking lfy ap1 double mutants in A. thaliana.

138 WT, med12, aux1-7 and med12 aux1 single and double mutants in response to sucrose and/or N-1-naphthy

139 in decreased proteolytic stability of the Rz double mutants in vivo Unlike the wild type, in which ly

140 This was revealed by genetic studies with double mutants including carotenoid isomerase (yofi), a

141 The adg1suc2 double mutant increases glucose plus sucrose content in

142 complete abolition of AMY3 (in the amy3 ss4 double mutant) increases the number of starch granules p

143 This effect is absent in kif3a;IFT double mutants, indicating that IFT proteins have ciliar

144 Nevertheless, the LMP1/LMP2A double mutant induces lymphomas in approximately half of

145 g synergistic effect with the PR-D212N,F234S double mutant, inducing an astonishing 200 nm red shift

146 ring reproductive development, we analyzed a double mutant, ire1a ire1b.

147 ngle mutants are viable, but the smd1a smd1b double mutant is embryo-lethal, indicating that SmD1 fun

148 Moreover, the smd1b mtr4 double mutant is embryo-lethal, suggesting that SmD1 is

149 The cca1-1 lhy-20 double mutant is epistatic to sic-3, indicating the LHY

150 tivity to Ro 8-4304, and the prmt5-1 chs3-2D double mutant is lethal.

151 ether the previously described phenotypes of double mutant kea1kea2 plants are due in part to defects

152 ed with single-mutant Kit(V558Delta/+) mice, double-mutant Kit(V558Delta;Y567F/Y567F) knock-in mice l

153 The double mutant knocks out the RNA-splicing arm of the UPR

154 he parent strain efficiently outcompeted the double mutant lacking HMW1 and HMW2.

155 B and found that the deletion mutants and a double mutant lacking the C94-C111 and C95-C112 disulfid

156 We previously described HSV double mutants lacking both gE and US9 that failed to tr

157 lso seems to be involved, since dpe2-1/phs1a double mutants lacking both PHS1 and the cytosolic DISPR

158 cking or overexpressing ESV1 or LESV, and of double mutants lacking ESV1 and another protein necessar

159 Hence, we could select double mutants lacking the T-DNA already in the first of

160 estingly, the endosperm oil of myb115 myb118 double mutants lacks omega-7 FAs.

161 Tet2/Nras double-mutant leukemia showed preferential sensitivity t

162 Here we describe a new collection of 275 double-mutant lines derived from a library of single-mut

163 transfer DNA insertion mutant lpeat2 and the double mutant lpeat1 lpeat2 showed impaired growth, smal

164 While castration of dystrophin and utrophin double mutant (mdx-dm) mice to mimic pre-pubertal nadir

165 pathology in the severe dystrophin/utrophin double mutant (mdx:utr (-/-)) mouse model of DMD.

166 ar cancer stem cell population in Pten/Trp53 double mutant medulloblastomas.

167 Although in Apc-Grp78 double mutant mice the Wnt signature was lost, these int

168 mphatic vascular defects seen in Fgfr1/Fgfr3 double mutant mice, while HK2 overexpression partly resc

169 replication stress in vivo, we used Hus1/Atm double mutant mice.

170 during early-life (pre-puberty, CRHOEdev) in double-mutant mice (Camk2a-rtta2 x tetO-Crh) and tested

171 electro-olfactogram (EOG) recordings on the double-mutant mice, NCKX4(-/-);CNGB1(DeltaCaM), which ar

172 ifespan was extended in Atg7 cKO; SOD1(G93A) double-mutant mice.

173 g were boosted in tumor tissues of Apc Olfm4 double-mutant mice.

174 eletion of Oxtr in Oxtr(-/-):Avpr1alpha(-/-) double-mutant mice.

175 ation, in the renal tubules of Tsc1 and rpS6 double-mutant mice.

176 Both double-mutant models developed high-penetrance AML, alth

177 Spry1(-/-) and Spry2(-/-) double mutant mouse embryonic fibroblasts exhibited decr

178 elf elevation-80% of Pax9(del/del);Wise(-/-) double-mutant mouse embryos exhibit rescued palatal shel

179 Of note, the double mutant msn2Deltamsn4Delta exhibited a severe grow

180 Here, we used PA as a double mutant (N682A, D683A; mPA) which cannot bind to t

181 nsitive1 dgd1 and allene oxide synthase dgd1 double mutants no longer exhibited the short inflorescen

182 e determined the solution NMR structure of a double mutant of CsgE (W48A/F79A) that appears to be sim

183 These findings allowed us to produce a double mutant of Hhn2b that shows nanomolar inhibition o

184 The double mutant of IAA20 and its closest homolog IAA30 for

185 observed in LOTUS-KO mice was rescued in the double mutant of LOTUS- and NgR1-KO mice.

186 sticity of the high-efficiency frameshifting double mutant of the 26 nt potato leaf roll virus RNA ps

187 Double mutants of ago1 and coronatine insensitive1, the

188 In contrast, double mutants of arf-1.1 and ncs-1 had an intermediate

189 ellular activity against both the single and double mutants of EGFR, demonstrating target engagement

190 est this, we generated Foxg1(-/-);Wnt8b(-/-) double mutants of either sex and found that the morpholo

191 The single and double mutants of His122 and Tyr125 not only enhanced th

192 The double mutants of PGK3 and the triose-phosphate transpor

193 test this hypothesis by functional analysis, double mutants of the flounder SLC34A2 protein were cons

194 d type sequence, involving mostly single and double mutants, or a combinatorially complete subgraph i

195 essed in tan1 air9 significantly rescued the double mutant phenotype in all three respects.

196 acids, TAN1-DeltaI-YFP, failed to rescue the double mutant phenotype, while TAN1 missing a conserved

197 racterized by analyzing expression patterns, double mutant phenotypes, promoter-GUS fusions and expre

198 ion antagonistically affect the alpha Aurora double mutant phenotypes.

199 In the grik1-2 grik2-1 double mutant, phosphorylation of SnRK1.1 was reduced, b

200 SUMO1/2 double mutant plants are nonviable, underlining the impo

201 Analysis of double mutant plants defective in different combinations

202 on, as SL-deficient and ethylene-insensitive double mutant plants display essentially additive phenot

203 -type plants under low nitrate conditions in double mutant plants in bt2 and its closely related homo

204 a isolates was also compromised in gae1 gae6 double mutant plants.

205 In conjunction with this defect, double-mutant plants accumulate significant amounts of l

206 We found that the ala1/ala2 single- and double-mutant plants exhibited enhanced disease suscepti

207 However, tex1 mos11 double-mutant plants show marked defects in vegetative a

208 mutant failed to produce DON, the tri10 pde2 double mutant produced a significantly higher level of D

209 The ptaA/pvdN double mutant produced exclusively the glutamic acid for

210 utant (ex1/ex2), and an apoplastic signaling double mutant (rbohD/F) revealed that tAPX and EXECUTER

211 In the exo1Delta sgs1Delta double mutant, resection was barely detectable, yet it o

212 Significantly, the double mutant restored the hydrolysis step to values sim

213 Most double mutants restored dopamine response to wild-type l

214 e tail point mutants with those of symmetric double mutants revealed that a single methylated H3K36 p

215 ck-resistant and loss-of-function single and double mutants revealed that each AHAS and IPMS isoenzym

216 e spxA1 strain as background for creation of double mutants revealed that four of the five genes inac

217 with the synergistic growth phenotype of the double mutant rh50-1 gun1-102, suggest that RH50 and GUN

218 For example, serk1serk3 double mutant roots are insensitive toward brassinostero

219 ots is similar to that of the serk1-3serk3-2 double mutant roots.

220 her number of negative interactions than the double mutant SGA screens and uncovered additional genet

221 udied the Arabidopsis (Arabidopsis thaliana) double mutant shaven3 shaven3-like1 (shv3svl1), which wa

222 Foxg1(-/-);Wnt8b(-/-) double mutants show a substantial rescue of the Foxg1(-/

223 k3 and mpk6 single mutants and the mpk3 mpk6 double mutants show enhanced freezing tolerance, whereas

224 Remarkably, only the HA double mutant showed a significantly increased pathogeni

225 The glk1 glk2 double mutant showed enhanced resistance to Pseudomonas

226 In contrast, the DeltampkC DeltasakA double mutant showed highly attenuated virulence, with a

227 2, and hec3 single mutants and the hec1 hec2 double mutant showed hyposensitivity to light-induced se

228 The amino acid content of leaves from the double mutant showed marked reduction in aspartate when

229 ty of hlb1, and analyses of ahlb1/ min7/ben1 double mutant showed that hlb1 and min7/ben1 operate in

230 d no visible phenotypes, whereas clpt1 clpt2 double mutants showed delayed development, reduced plant

231 in mature plants and developing embryos and double mutants showed multiple changes in morphology rel

232 Analysis of double mutants showed no additive effects between mutati

233 Zebrafish prps1a mutants and prps1a;prps1b double mutants showed similar morphological phenotypes w

234 to wild-type controls, Hus1(neo/neo)Atm(-/-) double mutants showed striking MMC hypersensitivity, con

235 Typical double mutants showed stunted growth of aerial and root

236 nitrogen fixation, with the Ljein2a Ljein2b double mutant showing severely reduced nitrogen fixation

237 ugar/energy sensing, and the grik1-1 grik2-1 double mutant shows growth retardation under regular gro

238 omain separation and cell death, whereas the double mutant significantly increased the TM domain sepa

239 ped recombination, suggesting that Apc-Grp78 double mutant stem cells had lost self-renewal capacity.

240 pment, the roots of ein3 and eil1 single and double mutants still respond to ethylene in light-grown

241 re able to colonize more frequently than the double mutant strain lacking HMW1 and HMW2.

242 differences were examined in the single and double mutant strains, and the virulence of select strai

243 mal defects were exacerbated in RING peroxin double mutants, suggesting distinct roles of individual

244 ey show that the resulting Deltaisc Deltasuf double mutants supplemented with mevalonate can grow slo

245 Furthermore, atgsnor1-3 nox1-1 double mutants supported greater bacterial titres than e

246 all three point mutants and >90% for the WRN double mutant (T1024G/T1086G) relative to normal B-form

247 By use of a noncovalent double mutant (T790M/L858R and T790M/del746-750) selecti

248 Tau transgenic mice (P301L line) and created double mutant (TauXDrp1+/-) mice.

249 ats and after genetic elimination of TGR5 in double mutant TGR5(-/-) ;Pkhd1(del2/del2) mice.

250 more severely compromised in foxn4 and foxj1 double mutants than in single mutants.

251 s We obtained a L. japonicus Ljein2a Ljein2b double mutant that exhibits complete ethylene insensitiv

252 We used norpA(P24) cry(02) double mutants that are circadianly blind in low light a

253 wild-type reversal frequency are restored in double mutants that are defective in both EPS production

254 Here, we show by analysis of conditional double mutants that the two T-box transcription factor g

255 identify a Chimaerin (CHIN-1)- Furin (KPC-1) double-mutant that severely disrupts assembly.

256 wild-type MA with its two budding deficient double mutants, that is, T41I/T78I and Y28F/Y67F.

257 In the S200V/S201V double mutant, the proton affinity of E286 is increased,

258 )/receptor activator of NF-kappaB-transgenic double mutants, the dentin phenotype, notably in the roo

259 Nevertheless, in tex1 mos11 double-mutants, the mRNA export defect was clearly enhan

260 eletion constructs were transformed into the double mutant to assess which regions of TAN1 are requir

261 able to grow heterotrophically, we find the double mutant to be embryo lethal.

262 We use the tan1 air9 double mutant to discover new functions for TAN1 and AIR

263 y showing enhanced resistance of a glk1 glk2 double mutant to Hyaloperonospora arabidopsidis.

264 WT, med12, aux1-7 and med12 aux1 single and double mutants to sucrose and application of auxin trans

265 the Arabidopsis hypersensitive bzip19 bzip23 double mutant under Zn deficiency.

266 ed, growth of nrt1.5-5 and skor-2 single and double mutants under different K/NO3 (-) regimes reveale

267 e were unable to generate fkp40(-)/afkp80(-) double mutants, unless one of the A/FKPs was expressed e

268 Moreover, an analysis of ahk double mutants using CycB1;1:GUS/ahk introgressed lines

269 analyzed perturbations caused by single- and double-mutant variants using steady-state kinetics, high

270 macrophages is dramatically increased during double-mutant virus infection and correlates with faster

271 The pgk1.1 pgk3.2 double mutant was bigger than pgk3.2 and displayed an in

272 A DeltaciaRDeltaargB double mutant was completely restored for the gtfP gene

273 emical levels, whereas the ndufs8.1 ndufs8.2 double mutant was devoid of detectable holo-CI assembly/

274 mutant is a sporophytic trait, and when the double mutant was grown at ET, defects appeared in the s

275 muL L(-1) CO2 The reduction in growth of the double mutant was not linked to a reduction in photosynt

276 ced resistance to PcBMM of the agb1-2 esk1-7 double mutant was not the result of the re-activation of

277 hese cells, the Deltafkp40(-)/Deltaafkp80(-) double mutant was now readily obtained.

278 ase function, and the Na(+) affinity of this double mutant was reduced even more than in single mutan

279 prolonged quiescent phase of the pyl8-1pyl9 double mutant was reversed by exogenous IAA.

280 Growth of a sitA mntH double mutant was severely reduced under Mn(2+) limitati

281 e gln1;2 single mutant and the gln1;1:gln1;2 double mutant was significantly impaired irrespective of

282 Growth and viability of phr1phl1 double mutant was significantly reduced in phosphate-dep

283 In connection with the characterized double mutants, we discuss the generation of starch gran

284 Through the analysis of single and double mutants, we found that the synthetic interaction

285 utant or an endocytic-defective Y658F-VE-Cad double mutant were both able to rescue TEER independentl

286 nges in pathogenesis and transmission of the double mutant were evaluated in pigs.

287 s4 triple mutant and various combinations of double mutants were generated and metabolically analyzed

288 KNOX genes STM and BP were overexpressed in double mutants whereas CLV3, WUSCHEL and AS1 were repres

289 n of endothelial cells is compromised in the double mutant, whereas excessive production of myogenic

290 lso restores starch synthesis in the ss3 ss4 double mutant, which lacks STARCH SYNTHASE 3 (SS3) in ad

291 he corresponding values for the pf3; cnk11-6 double mutant, which lacks the nexin-dynein regulatory c

292 A CFTR double mutant with an extracellular gate mutation plus a

293 d of mutations resulted in (E5K,E8K)MfVIA, a double mutant with greater positive surface charge and g

294 other mutants were remarkably silent, but a double mutant with His(273) and His(274) exchanged for a

295 wk) and allows simultaneous construction of double mutants with high efficiency, exponentially decre

296 al and colon tumors resulted in GH-deficient double mutants with markedly decreased tumor number and

297 The double mutant Y420A/L452A nearly eliminated the effects

298 A VE-cadherin double mutant (Y658F, Y731F) expressed in endothelial ce

299 terize an Arabidopsis (Arabidopsis thaliana) double mutant, yellow stripe1-like yellow stripe3-like (

300 rvae, whereas juvenile and adult pax7a/pax7b double-mutant zebrafish display a severe decrease in mel