1 n VLM and NTS, 74% and 42% respectively were
double labeled.
2 Significantly, very few neurons (<2%) were
double-labeled.
3 n is deuterated and the C-terminal domain is
double-labeled.
4 he IC and auditory cortex did not reveal any
double labeling.
5 enalidomide treatment using CD138(+)/IRF4(+)
double labeling.
6 ected to both the AI and the LO as judged by
double-labeling.
7 assay was performed with a primary antibody,
double-labeled amplicons, and fluorophore-labeled strept
8 Our
double-labeling analyses of intraneuronal Abeta and CD40
9 Finally,
double-labeling analysis of CTb + Fos showed that contex
10 Evidence from both
double label and single axon studies, however, suggests
11 Combining
double labeling and confocal microscopy analyses, we fou
12 Fifteen of these were confirmed with
double labeling and microscopy, including the transcript
13 otope probing (Raman-SIP) using a single and
double labeling approach.
14 nstrated by cell lineage tracing and mineral
double-labeling approaches that murine PDL progenitor ce
15 hree taste cell types expresses this enzyme,
double-label assays were performed with antisera directe
16 Some
double-labeled BDA/VGAT varicosities were seen apposed t
17 content and the numbers of insulin and BrdU
double-labeled beta cells in the islets.
18 To prove this hypothesis, we tracked
double-labeled BMSCs in implantation sites created in nu
19 Some BrdU-labeled cells were also
double labeled by antibodies to glial-specific (antikera
20 When
double labeled by in situ hybridization, these neurons c
21 Double-labeled cancer cells were imaged at the cellular
22 Double-labeled cells are injected by various methods.
23 hat, for all three primary sensory cortices,
double-labeled cells were extremely rare, indicating tha
24 Generally,
double-labeled cells were in expected high-frequency ton
25 abeled cells in LH co-expressed SS, while no
double-labeled cells were observed in IC.
26 Numerous
double-labeled cells were observed throughout the same a
27 Elsewhere,
double-labeled cells were very scarce, making up approxi
28 cells, there is a considerable population of
double-labeled cells with approximately 30% of each popu
29 ess can be observed to emanate from BrdU/DCX
double-labeled cells.
30 To do this, we used
double-labeling combinations of Fos immunocytochemistry
31 melatonin receptor localization in the OPL,
double-label confocal immunohistochemistry for Mel1a or
32 Extensive
double-labeling crossover studies have been conducted.
33 e mechanism was obtained through an isotopic
double-labeling crossover study.
34 g to bones through the use of differentially
double-labeled derivatives.
35 BrdU staining, providing a valuable tool of
double labeling DNA synthesis, e.g., for tracking the tw
36 Conventional molecular beacons require
double-labeled DNA sequences, which are costly and time-
37 ch DNA molecule, thereby establishing short,
double-labelled DNA molecules as probes of 3D orientatio
38 lowing the principle of proportionality, and
double-labeled duplex DNA was synthesized.
39 Double label experiments with b-sCD44 or -p b-sCD44 and
40 ochemical methods to test the feasibility of
double labeling experiments during development.
41 Double labeling experiments indicate that the neurochemi
42 Double labeling experiments, by either in situ hybridiza
43 bone formation rates in in vivo fluorochrome
double labeling experiments.
44 Double-label experiments did not confirm colocalizations
45 Double-label experiments showed that the SIFamide-immuno
46 Double-label experiments showed that these bipolar cells
47 Double-labeling experiments (biocytin backfill x seroton
48 Double-labeling experiments confirm that NADPH-d, the mo
49 cell the "distal Dab1-immunoreactive cell."
Double-labeling experiments demonstrate that the distal
50 Double-labeling experiments further demonstrate that V1
51 c gut-associated lymphoid tissue at 14 days;
double-labeling experiments indicated that 93.5% of the
52 Double-labeling experiments reveal NMB is expressed in a
53 Double-labeling experiments showed that the dendritic an
54 in the control of food intake, we performed
double-labeling experiments to evaluate the potential ov
55 tly labeled retinal terminals was counted in
double-labeling experiments using confocal microscopy an
56 Double-labeling experiments using probes for previously
57 Single- and
double-labeling experiments were performed to establish
58 Double-labeling experiments with ArINTs and ArINTstBu (T
59 When mAb-GlyRbeta was used in
double-labeling experiments with GlyRalpha1-, alpha2-, a
60 then determining the relative proportions of
double-labeled FF and FB neurons in an area intermediate
61 the lateral septum (LS), whereas only a few
double-labeled fibers were found in other brain areas in
62 Double-label fluorescence for Abeta42 and pT231 revealed
63 Rats had
double-label fluorescence IMHC for localization of Abeta
64 Here, using
double-label fluorescence in situ hybridization for the
65 Double-label fluorescence in situ hybridization reveals
66 recipitation assays and co-localization with
double-label fluorescence microscopy.
67 Using
double labeling fluorescent histochemistry with confocal
68 Third, using the technique of
double-labeled fluorescent immunocytochemistry, the rela
69 amined using myeloperoxidase (MPO) assay and
double-labeling fluorescent immunohistochemical analysis
70 They were also
double labeled for GFP and RPE65 or MITF.
71 maniculatus, and Peromyscus polionotus, and
double labeled for the expression of ERalpha and AVP imm
72 As expected,
double labeling for ATF-3, a marker of cell bodies with
73 Double labeling for GAD67 and GHRHR in vitro and in vivo
74 The current investigation used
double labeling for NADPHd and Fos-like immunoreactivity
75 Double labeling for Prox1 and cell-type-specific markers
76 bserved in dendritic profiles, verified with
double labeling for the dendrite-specific marker microtu
77 al cells were identified as keratinocytes by
double labeling for WNV antigen and keratin 10.
78 The proportions of TH cell profiles
double-labeled for ChAT or VIP significantly increased b
79 -estradiol, the number of perirhinal neurons
double-labeled for ER-beta/GABA was reduced by 28% (P<0.
80 ctory organs were cryosectioned (10 microm),
double-labeled for Galpha(olf), Galpha(q), or PLC140, an
81 cells in the inner nuclear layer (INL) were
double-labeled for TH immunoreactivity.
82 The majority of FG-filled cells
double-labeled for TH were found in the dorsocaudal A10
83 Double-labeling for GAD and synaptophysin confirmed that
84 samples that are generally different from a
double-labeled FRET sample, or by the use of sophisticat
85 Double labeling further reveals that the majority of Cck
86 Light and electron microscopic
double labeling further showed that the VGLUT1 subtype o
87 cumented using the characteristic pattern of
double-labeled gene trees.
88 Targets exposed to
double-labeled granzyme B-serglycin complexes show solel
89 and protein immunofluorescence (IF)-RNA FISH
double labeling (
IF/FISH).
90 Double-label IHC experiments with tyrosine hydroxylase a
91 Using
double-labeled immune-fluorescence confocal microscopy,
92 Double labeling immunocytochemical studies confirmed tha
93 using retrograde tract tracing combined with
double label immunocytochemistry and found that neuron p
94 specimens were analyzed by Western blot and
double label immunocytochemistry.
95 Double-label immunocytochemistry detected EAAT2 in OLs b
96 epileptic cortical samples were analyzed by
double-label immunocytochemistry for coexpression of neu
97 Double-label immunocytochemistry indicates that Bex-ir i
98 Single- and
double-label immunocytochemistry showed a significantly
99 We utilized
double-label immunocytochemistry to identify the phenoty
100 We applied single- and
double-label immunocytochemistry to normative frontal or
101 alogs and also determined their phenotype by
double-label immunocytochemistry using type-specific mar
102 e cell populations and connectivity, we used
double-label immunocytochemistry with antisera to differ
103 ped in a teleost (zebrafish, Danio rerio) by
double-label immunocytochemistry.
104 Double label immunofluorescence analysis of 3T3-L1 adipo
105 hat controls feeding and energy expenditure,
double label immunofluorescence studies were employed.
106 by using a combination of routine histology,
double-label immunofluorescence and in situ hybridizatio
107 Double-label immunofluorescence confocal studies show th
108 yelin unwinding and intramyelinic cysts, and
double-label immunofluorescence for 4-hydroxy-2-nonenal
109 Double-label immunofluorescence for BrdU and the protein
110 alpha-smooth muscle actin(+)) as assessed by
double-label immunofluorescence microscopy.
111 of hypocretin neurons was analyzed by using
double-label immunofluorescence of FosB/DeltaFosB with h
112 esame oil-treated animals were processed for
double-label immunofluorescence of GAD (a marker for GAB
113 Double-label immunofluorescence staining demonstrated th
114 In
double-label immunofluorescence staining, CD11c+, a mark
115 We used immunohistochemistry and
double-label immunofluorescence to characterize CD163(+)
116 We also used
double-label immunofluorescence to demonstrate colocaliz
117 We used
double-label immunofluorescence to visualize the distrib
118 Examining SIVE lesions, using
double-label immunofluorescence with antibodies against
119 Using
double-label immunofluorescence, GLT-1 was shown to colo
120 Using
double-labeling immunofluorescence analysis of intraneur
121 Double-labeling immunofluorescence histochemistry reveal
122 Double-labelling immunofluorescence showed a 95% co-loca
123 With
double-labeling immunogold electron microscopy (EM), we
124 Two
double-label immunohistochemical experiments were conduc
125 Double-label immunohistochemical procedures demonstrated
126 Double label immunohistochemistry revealed that hemorrha
127 Using
double label immunohistochemistry we found that DsRed fl
128 We demonstrate using
double labeling immunohistochemistry that Group II metab
129 of stress-responsive LC neurons we performed
double-label immunohistochemistry for TH and Fos.
130 d precursor protein (APP) has been observed,
double-label immunohistochemistry revealed no evidence o
131 Double-label immunohistochemistry revealed that acute re
132 In the present study, we used single- and
double-label immunohistochemistry to investigate the rel
133 Double-label immunohistochemistry using antibodies again
134 tacts between identified cell types, we used
double-label immunohistochemistry, in vivo retrograde tr
135 By
double-label immunohistochemistry, we found close apposi
136 of AD and control patients using single- and
double-label immunohistochemistry.
137 utamate) immunoreactivity (FOS/PAG-IR) using
double-label immunohistochemistry.
138 ne (BrdU) labeling of newly generated cells,
double-labeling immunohistochemistry and TUNEL labeling
139 Double-labeling immunohistochemistry indicated that PR-i
140 Double-labeling immunohistochemistry was performed with
141 Double-labelling immunohistochemistry and confocal micro
142 ing to identify corneal afferent neurons and
double label in situ hybridization and/or immunohistoche
143 Protein
double labeling in aqueous buffer at physiological pH, t
144 lateral lemniscus (VNLL and INLL), with some
double labeling in ipsilateral lateral and medial superi
145 tion neurons exhibited a higher incidence of
double labeling in the superficial dorsal horn.
146 s before sacrifice, we have used single- and
double-label in situ hybridization.
147 r postnatal stages of C57BL/6J mice by using
double-label in situ hybridization.
148 e projecting to accumbens shell, with higher
double-labeling in the ipsilateral projection than in th
149 In both species, lack of overt
double labeling indicated that the ChAT(+) and urocortin
150 Less than 1% of cells were
double-labeled,
indicating that the populations of cells
151 Mouse LGs were processed for single- and
double-labeled indirect immunofluorescence studies and e
152 Different sets of
double labels initially located 26-27 A apart in the min
153 When duplex TAR DNA-TAR RNA formed,
double labels initially located 27.5 A apart at the 3'-
154 We
double labeled Ipc axons and their presumptive postsynap
155 now applied single-molecule FRET to Cy3, Cy5
double-labeled LacI-DNA loops diffusing freely in soluti
156 In the absence of complementary TAR RNA,
double labels located in both the upper and the lower st
157 of fine processes called telodendria and, in
double-labeled material, Cx36 plaques were located preci
158 study femurs were isolated from genetically
double-labeled mBSP9.0Luc/beta-ACT-EGFP transgenic mice
159 We employed a
double-label method to map the ascending projections of
160 ural level, we develop and apply a "two-tag"
double-labeling method to label LT11's dendrites and the
161 eparate populations of synapses, we employed
double-labeling methods.
162 f these compounds was readily converted to a
double-labeled mixed-chain phosphatidylcholine applicabl
163 abeled motoneurons and higher proportions of
double-labeled motoneurons than untransected rats.
164 The distance distributions between three
double-labeled mutants, in the collapsed transient state
165 co-expressed enkephalin (Enk), and Ucn 3/Enk
double-labeled nerve fibers and terminals were observed
166 ope, and scored for the number of single and
double labeled neurons.
167 ed with no laminar segregation, we found few
double-labeled neurons ( approximately 5% of each singly
168 However,
double-labeled neurons also exhibited a preferential dis
169 fferent tracers were injected in SII and MI,
double-labeled neurons appeared above and below the laye
170 tral PBN resulted in a greater percentage of
double-labeled neurons in BNST and CeA compared to cauda
171 d in significant increases in the numbers of
double-labeled neurons in both the NST and RF, suggestin
172 These differential distributions of
double-labeled neurons in the NST and RF suggest a role
173 Double-labeled neurons in the NTS were located primarily
174 sion as well as the number of FluoroGold/Fos
double-labeled neurons in the ventral Vi/Vc (P<0.05).
175 Minor PACAP projections with scattered
double-labeled neurons originated from the parabrachial
176 ime-lapse confocal microscopy of individual,
double-labeled neurons revealed a coincident, activity-d
177 sker representations of SII and MI to detect
double-labeled neurons that would indicate that some SI
178 of cortical GABA, ER-beta, and ER-beta/GABA
double-labeled neurons was examined.
179 For SS, the percentage of
double-labeled neurons was more forebrain site specific
180 No
double-labeled neurons were found in the dorsal Vi/Vc an
181 Double-labeled neurons were found in the reticular, raph
182 Overall,
double-labeled neurons were most numerous in the caudal
183 In the NST,
double-labeled neurons were most numerous in the rostral
184 Moderate numbers of
double-labeled neurons were observed following combined
185 However, only a few
double-labeled neurons were occasionally observed after
186 ric acid stimulation, there was a cluster of
double-labeled neurons with distinctive large soma in th
187 response to quinine, there was a cluster of
double-labeled neurons with much smaller soma in the int
188 ese animals was examined for the presence of
double-labeled neurons, i.e., those whose axons had rege
189 trogen-mediated differences in the number of
double-labeled neurons.
190 NST and CeA exhibited significant numbers of
double-labeled neurons.
191 colocalized and contained some percentage of
double-labeled neurons.
192 ed neurons in the claustrum, as well as many
double-labeled neurons.
193 d neurons in ipsilateral DEn, including many
double-labeled neurons.
194 We hypothesized that the
double labeling of an adenovirus with fluorescent protei
195 Double labeling of CB2R and glutamine synthetase shows t
196 4-5-day hatchling chicks by using single and
double labeling of fibers and terminals with biocytin co
197 al surgery activated brain CRF neurons using
double labeling of Fos/CRF in naive rats.
198 The
double labeling of ganglion neurons indicates their site
199 Indeed,
double labeling of miR156 showed a meristem-specific pat
200 Transgenesis and
double labeling of NTS and HCRT neurons showed that NTS
201 Double-transgenic mice showed 55%
double labeling of periurethral neuroendocrine cells exp
202 rviving ganglion cells were quantified after
double labeling of retinal tissue with TUNEL and Brn3a.
203 Double labeling of sGC with neuronal nitric oxide syntha
204 Double labeling of sGC with neuronal nitric oxide syntha
205 Double labeling of the N- and C-terminally tagged peripl
206 g context-induced renewal tests by measuring
double labeling of the retrograde tracer cholera toxin s
207 Double labeling of TSH4 with the ramosa2, branched silkl
208 Although
double-labeling of microglia with Iba1 and ED1 revealed
209 Double-labeling of neurons in which GFP was driven by C3
210 First, we measured
double-labeling of the neuronal activity marker Fos with
211 pha-CreER(T2) : R26R-YFP transgenic mice, we
double labeled OPCs and traced their fate in the postnat
212 process through crossover experiments using
double-labeling (
oxo and phosphine).
213 The
double-labeled peptides have amide I' IR spectra that sh
214 From triple-labeled tissue, we found that
double-labeled PHA-L (+)/VGluT2 (+) axon terminals forme
215 These mGluR1alpha
double-labeled populations are not likely to overlap sin
216 A dense plexus of
double-labeled prodynorphin/proNKB-ir fibers was found w
217 nation of methods allows for rapid access to
double-labeled proteins with a minimum of unnecessary ch
218 ed an iododeoxyuridine and bromodeoxyuridine
double labeling protocol for use in the developing embry
219 of inflammatory cells within lesions using a
double labelling protocol which employed anti-PLP in add
220 We have used both single- and
double-label protocols to investigate the relation betwe
221 triple-labeled PV(+) /CR(+) /SMI32(+) ; (b)
double-labeled PV(+) /CR(+) ; and (c) single-labeled CR(
222 movement was quantified by image analysis of
double-labeled retinas examined with confocal microscopy
223 Double-label retrograde anatomical tracing techniques we
224 Double labeling revealed that 30.2% of nodose neurons ex
225 Immunofluorescence
double-labeling revealed a punctate pattern for ENT1 clo
226 Double-labeling revealed that only two regions of the mo
227 By means of
double-labeling RNA in situ hybridization in mice, we sh
228 n a wide system of conditions using the same
double-labeled sample from which the FRET efficiency its
229 Analysis of the
double-labeled sections indicates that NTPDase2 immunore
230 alitative abnormalities, with bone turnover (
double labeling)
seen in all specimens.
231 Double labeling showed that neuronal nitric oxide syntha
232 Double labeling showed that the thalamic valop populatio
233 Double labeling showed that type 5b cone bipolar cells e
234 Double labeling showed these to be both astrocytes and o
235 r, immunofluorescence of BRCA1 and nucleolin
double-labeling showed colocalization in both nucleoli a
236 Double labeling shows coincidence of STIM1 and STIM2 wit
237 gher density of synaptic PKMzeta labeling in
double-labeled spines correlated with both faster task a
238 These
double-labeled spines had larger synapses, as measured b
239 Within this population of
double-labeled spines, aged monkeys compared with young
240 Here, we used a
double-labeling strategy (varying both the distance betw
241 Immunofluorescent
double labeling studies additionally reveal the prominen
242 Double labeling studies confirmed that the alpha-syn::GF
243 Double labeling studies showed that nicotine induced c-f
244 Immunohistochemical
double labeling studies with YFP and serotonin antisera
245 Double-label studies show that all cells immunoreactive
246 Double-label studies showed these inputs ended directly
247 Double-labeling studies demonstrated the localization of
248 Double-labeling studies revealed that early OL progenito
249 In
double-labeling studies using confocal microscopy, fluor
250 Double-labeling studies with antibodies to phosphorylate
251 In this
double-labeling study, we placed a green axonal tracer i
252 d with the region that showed FluoroGold/Fos
double labeling,
suggesting reciprocal connections betwe
253 retrogradely labeled neurons and significant
double labeling,
suggesting that cortical projections sp
254 coefficients (slopes) between serum PTH and
double-labeled surface (P=0.02) or osteoblast surface (P
255 rmone (PTH), which is indicated by decreased
double-labeled surface and osteoblast surface (P<0.001).
256 Immunohistochemical
double-labeling technique with Fos and markers for norad
257 Y-NB (Lucifer yellow-Neurobiotin) retrograde
double-labeling technique, in conjunction with specific
258 This was investigated with immunofluorescent
double-labeling techniques to coregister PV- and CB-expr
259 d that intracellular flora by using the same
double-labeling techniques to identify Fusobacterium nuc
260 Using
double-labeling techniques, we established that these in
261 Double labeling the brainstem sections revealed that irI
262 ons of the RVM and caudal pons and performed
double labeling to evaluate the expression of alpha-7 an
263 En face
double labeling using Ki-67 and progenitor markers revea
264 ions were processed for immunohistochemistry/
double labeling using patient sera/cerebrospinal fluid a
265 la, and midbrain as CRH neurons, although no
double labeling was found.
266 Much less
double-labeling was associated with injections into eith
267 function (bone formation) after tetracycline
double-labeling was performed by fluorescence microscopy
268 Commissural divergence, assessed by
double labeling,
was less than 3% in each area.
269 abeling and with preembedding immunogold for
double labeling,
was localized in cell bodies with ultra
270 ot in the MICG, and some of these cells were
double labeled with an antiserum to the glial protein S-
271 readily identified in the aorta and could be
double labeled with antibodies to CD11c and antigen-pres
272 Brains were
double labeled with fluorescence in situ hybridization o
273 tures that in the human VZ/SVZ, cells can be
double labeled with RG markers and calretinin (CalR) and
274 Cell death was quantified by
double labeling with a membrane impermeable dye and 4',6
275 Double labeling with anti-PECAM1 antibody and anti-place
276 Double labeling with anti-PECAM1 antibody and one of thr
277 ype of BrdU-positive cells was identified by
double labeling with antibodies to neuronal or glial mar
278 In
double labeling with calretinin and parvalbumin, few neu
279 ricular nucleus of the hypothalamus (PVH) by
double labeling with markers expressed in viruses inject
280 Double labeling with neuropeptide Y (NPY), a marker for
281 Double labeling with other molecular markers confirmed t
282 Double labeling with parvalbumin antibodies in monkey re
283 Double labeling with SNAP25 and calbindin antibodies dem
284 Double labeling with the ganglion cell marker RBPMS demo
285 esence of small, clear synaptic vesicles and
double labeling with the presynaptic markers synaptophys
286 Double labeling with the vesicular glutamate transporter
287 o functional recovery, sequential retrograde
double labeling with two fluorescent dextran amines was
288 Double labeling with various glutamate receptor subunit
289 Double labelling with markers for specific classes of en
290 , ii) single-stained with anti-APP, and iii)
double-labeled with anti-APP and AT8.
291 rols (n=5), c-Fos-positive cells and neurons
double-labeled with c-Fos and beta-endorphin, enkephalin
292 Moreover, neurons
double-labeled with c-Fos and choline acetyltransferase
293 on (n=5), c-Fos immunoreactivity and neurons
double-labeled with c-Fos and either enkephalin or 5-HT
294 ulation), c-Fos immunoreactivity and neurons
double-labeled with c-Fos, an immediate early gene and t
295 % of the remaining Rbpms-positive cells were
double-labeled with FG.
296 The IL1beta-IR cells
double-labeled with glial fibrillary acidic protein (GFA
297 Immunohistochemistry was performed by
double-labeling with anti-human MMP1 and collagen type I
298 t 14.6% of CT-beta-labelled PVN neurons were
double-labelled with AVP.
299 Double labeling (
with NeuN and GFAP) immunohistochemistr
300 pale and thick stripes; 10-27% of cells were
double labeled,
with most located in interpatches.