ライフサイエンスコーパス: doublecortin

1 d for BrdU and a marker of immature neurons, doublecortin.

2 ole for phosphorylation in the regulation of Doublecortin.

3 codes a novel 40 kDa predicted protein named Doublecortin.

4 es, which can be rescued by the knockdown of doublecortin.

5 expression of the neuronal markers Tuj1 and doublecortin.

6 AT1 colocalizes on bundled microtubules with doublecortin.

7 topia-like phenotype, reminiscent of loss of doublecortin.

8 These distinct properties combine to give doublecortin a unique function in microtubule regulation

9 dal but not stellate-cells co-localized with doublecortin - a marker of immature neurons - suggesting

10 After C cells, Doublecortin(+) A cells were the second-most common divi

11 Doublecortin, a component of the microtubule cytoskeleto

12 helin-1-treated mice, and cells reactive for doublecortin, a marker for immature neurons, were simila

13 murine piriform cortex express low levels of doublecortin, a marker for migratory and immature neuron

14 of DCN neurons in the adult rat that express doublecortin, a plasticity-related protein.

15 ed the immunocytochemical method to localize doublecortin, a protein associated with microtubules in

16 Subsets of BrdU+ precursors expressed Doublecortin, a protein found exclusively in migrating n

17 Doublecortin, along with the newly characterized mDab1,

18 Mutations in doublecortin also cause sporadic DC in females.

19 topathology and putative progenitor markers [doublecortin and calcium/calmodulin-dependent protein ki

20 These traits include enhanced expression of doublecortin and CaM kinase-like-1 (DCAMKL-1), Lgr5, CD1

21 ependent increases in progenitors expressing doublecortin and CaM kinase-like-1 (DCAMKL1), stem cells

22 Co-labelling with doublecortin and neuron-specific markers and BrdU in spi

23 arrest, induction of the neuroblastic marker doublecortin and of the neuron-specific intermediate fil

24 on of RhoA small GTPase and up-regulation of doublecortin and p35, which, in turn, modulate the actin

25 tors were unaffected, while raised levels of doublecortin and Proliferating Cell Nuclear Antigen (PCN

26 rd coexpressed the immature neuronal markers doublecortin and proliferating cell nuclear antigen but

27 Hippocampal neurogenesis, measured by doublecortin and proliferating cell nuclear antigen expr

28 dine-labeled) with various cellular markers; doublecortin and PSA-NCAM as the early neuronal marker,

29 (radial and horizontal) and three classes of doublecortin and PSA-NCAM-positive D cells (D1, D2, D3)

30 Expression of doublecortin and TUC-4 was associated with neurons in th

31 scopy to characterize microtubule binding by doublecortin and visualize its binding site.

32 mammary tumors had no effect on hippocampal doublecortin + and did not alter depressive-like behavio

33 oughout the dorsal hippocampus (positive for doublecortin) and expressed markers for astrocytes and f

34 nd microtubule-associated components such as doublecortin, and LIS1.

35 DF infusion also resulted in an induction of doublecortin- and Sox10 double-positive cells in the adu

36 (2 h) or Ki-67; immature neurons labeled by doublecortin; and adult-generated neurons labeled with B

37 Anti-doublecortin antibodies, such as the rabbit polyclonal A

38 onal nuclei protein (NeuN, a.k.a. Fox-3) and doublecortin antigens in the whole brain of chicks 2 day

39 cursor cells and young neurons, labeled with doublecortin, appeared to be lost equally from rostral a

40 nocytochemistry for newly born neurons using doublecortin, as well as double labeling using an additi

41 The finding that doublecortin associates with FIGQY-phosphorylated neurof

42 ncreased in old age, and the lower levels of doublecortin at old age in the hippocampus of Arg-61 mic

43 specificity was explained when we found that doublecortin binds between the protofilaments from which

44 Doublecortin binds selectively to 13 protofilament micro

45 AAV2/1-FGF2 injection enhances the number of doublecortin, BrdU/NeuN, and c-fos-positive cells in the

46 labeled with neuronal (beta-III-tubulin and doublecortin) but not RG markers [GFAP, vimentin, and BL

47 a microtubule-associated protein related to doublecortin, by Shu et al., Koizumi et al., and Deuel e

48 th a camelid antibody fragment show that the doublecortin C-terminal domain adopts the same well defi

49 Bax-knock-out, there was an accumulation of doublecortin, calretinin+, and neuronal-specific nuclear

50 ls were localized in the isthmus adjacent to doublecortin CaM kinase-like-1(+) putative progenitor ce

51 A mutation in LIS1 or doublecortin can lead to either classical lissencephaly

52 Mutations of doublecortin causing lissencephaly (R59H, D62N, and G253

53 of bromodeoxyuridine-positive, Ki-67(+), and doublecortin(+) cells in the subgranular zone of the den

54 in fibers in the cingular cortex and loss of doublecortin(+) cells in the subventricular zone and hip

55 Finally, phospho-FIGQY neurofascin and doublecortin colocalize in developing axon tracts and in

56 In the adult rostral migratory stream, doublecortin colocalizes in migrating neurons with a pho

57 des in RP1 diseases, and suggest that RP1, a doublecortin-containing microtubule associated protein,

58 Doublecortin contains a consensus Abl phosphorylation si

59 GluN2A subunit, unco-ordinated-5H3 (unc5H3), doublecortin, cyclo-oxygenase, sonic hedgehog and Disrup

60 erneuron marker Er81, the neuroblast markers doublecortin (DC) and Distalless-related homeobox (DLX),

61 A recently identified gene, doublecortin ( DCX ), is expressed in fetal brain and mu

62 Doublecortin (DCX) and doublecortin-like kinase (DCLK),

63 l-labeled for ERbeta and the new-cell marker doublecortin (DCX) and examined by electron microscopy.

64 nto neurons as assessed by the expression of doublecortin (Dcx) and other neuronal fate markers.

65 Doublecortin (DCX) and polysialylated neural cell adhesi

66 Mutations in doublecortin (DCX) are associated with intractable epile

67 Finally, we establish doublecortin (DCX) as a novel substrate of GSK3beta in t

68 Doublecortin (Dcx) defines a growing family of microtubu

69 andidate for this role is TgDCX, which has a doublecortin (DCX) domain and a TPPP/P25-alpha domain, b

70 le-associated ciliary protein containing the doublecortin (DCX) domain.

71 d microtubules, indicating that the putative doublecortin (DCX) domains in RP1 are functional.

72 They can be induced to become Doublecortin (DCX) expressing migrating neuroblasts by R

73 ecortin-like kinases (DCLKs), members of the doublecortin (DCX) family expressed in adult retinal gan

74 Mutations in the doublecortin (DCX) gene in human or targeted disruption

75 We have also cloned the X-linked mouse doublecortin (Dcx) gene.

76 Using doublecortin (DCX) immunocytochemistry, these growing de

77 Here we studied doublecortin (DCX) in cultured hippocampal and sympathet

78 Doublecortin (DCX) is a cytoskeletal protein that is pri

79 Doublecortin (DCX) is a microtubule-associated protein r

80 Doublecortin (DCX) is a microtubule-associated protein t

81 Doublecortin (Dcx) is a microtubule-associated protein t

82 Doublecortin (DCX) is a microtubule-associated protein t

83 Doublecortin (DCX) is an important microtubule-associate

84 Doublecortin (DCX) is one of the three genes found from

85 Doublecortin (DCX) is required for normal migration of n

86 Doublecortin (Dcx) is the causative gene for X-linked li

87 For example, doublecortin (Dcx) knockdown but not knockout shows a ne

88 Doublecortin (DCX) missense mutations are found in two c

89 2b (PHOX2B), tyrosine hydroxylase (TH), and doublecortin (DCX) mRNA in PB and BM of children enrolle

90 roximal enhancer of the neuron-specific gene doublecortin (Dcx) Once differentiation is induced, MEIS

91 een fluorescent protein under control of the doublecortin (DCX) or glial fibrillary acidic protein (G

92 ng a DsRed reporter under the control of the doublecortin (DCX) promoter (labeling immature neurons).

93 ptor (DTR) is expressed under control of the doublecortin (DCX) promoter, which allows for specific a

94 synthase kinase 3beta (GSK3beta) activity on doublecortin (DCX) revealing mechanistic details about t

95 onsible for the dephosphorylation of the MAP Doublecortin (Dcx) Ser 297 selectively at the "wrist" of

96 SVZ; also called subependymal zone) generate doublecortin (Dcx)(+) neuroblasts that migrate and integ

97 Mutations in the human doublecortin (DCX), a brain-specific putative signaling

98 ession of the microtubule-associated protein doublecortin (DCX), a marker of neurogenesis.

99 inase (TK) under control of the promoter for doublecortin (Dcx), a microtubule-associated protein exp

100 e measured the distribution of expression of doublecortin (DCX), a microtubule-associated protein, in

101 Doublecortin (DCX), a microtubule-associated protein, is

102 Doublecortin (DCX), a MT-associated protein (MAP) requir

103 Here we show that doublecortin (DCX), a widely used marker for newly gener

104 tudy the expression of the neuroblast marker doublecortin (DCX), and compared its expression pattern

105 rogenitor cells such as Nestin, Musashi, and Doublecortin (DCX), and of the granule cell lineage such

106 The potential role of doublecortin (Dcx), encoding a microtubule-associated pr

107 llapsin response mediator protein 4 (CRMP4), doublecortin (DCX), HuD, and NeuN expression was assesse

108 the expression of bromodeoxyuridine (BrdU), doublecortin (DCX), IGF-1 and IGF-1R at 7, 14 and 30 day

109 ic neurogenesis that is highly homologous to doublecortin (DCX), in the adult mouse brain.

110 rence to the microtubule-associated protein, doublecortin (DCX), that has been extensively used to id

111 these cells express the cytoskeletal protein Doublecortin (DCX), yet they are generated prenatally an

112 Glutamic acid decarboxylase 65 (GAD65)- and Doublecortin (Dcx)-expressing cells constitute major pro

113 was performed 14 days after distal MCAO and doublecortin (Dcx)-expressing cells in the subventricula

114 Doublecortin (DCX)-immunopositive cells (i.e. neuroblast

115 We reported previously that ablation of doublecortin (DCX)-immunopositive newborn neurons in mic

116 The morphology of doublecortin (DCX)-positive cells in fixed brain section

117 protein 2 (MAP2)-positive neurons (-24%) and doublecortin (Dcx)-positive neuroblasts (-21%), and incr

118 that resident astrocytes can be converted to doublecortin (DCX)-positive neuroblasts by a single tran

119 rosphere-derived cells to show that immature doublecortin (Dcx)-positive neurons are uniquely sensiti

120 e algorithms, we solved the 8 A structure of doublecortin (DCX)-stabilized MTs.

121 K), a protein that shares high homology with doublecortin (DCX).

122 localized with the immature neuronal marker, doublecortin (Dcx).

123 vation of the microtubule-associated protein doublecortin (DCX).

124 ses the X-linked lissencephaly gene encoding doublecortin (DCX).

125 red the volumetric changes and expression of doublecortin (DCX; an endogenous marker of the neuronal

126 dNoggin-treated R6/2 mice harbored migrating doublecortin-defined neuroblasts in their striata, and t

127 Although Doublecortin does not contain a kinase domain, it is hom

128 In addition, doublecortin does not itself oligomerise and does not bi

129 ntibody fragment specific for the C-terminal doublecortin domain affected microtubule binding, wherea

130 is suggests that the microtubule-interacting doublecortin domain observed in cryo-electron micrograph

131 binding and polymerization activities of the doublecortin domain, yet little is known regarding the e

132 oint mutation in DCDC2 (DCDC2a), a member of doublecortin domain-containing protein superfamily, caus

133 nase (Dclk), encodes a protein with similar "doublecortin domains" and microtubule stabilization prop

134 orders are located on a tandem repeat of two doublecortin domains.

135 terization of antibodies that bind to single doublecortin domains.

136 ion of non-centrosomal microtubules in these doublecortin-enriched distal zones.

137 In this study, we quantified the number of doublecortin expressing (DCX+) immature neurons and Ki-6

138 (SGZ) as well as their derivatives including doublecortin-expressing neuroblasts and immature granule

139 we observe adjacent but more differentiated doublecortin-expressing progenitors (type-2 cells) being

140 e, increased numbers of proliferating cells, doublecortin-expressing progenitors/neuroblasts, and ear

141 vated and result in increased numbers of the doublecortin-expressing type-2 cells.

142 e RA-dependent up-regulation of p21/Cip1 and doublecortin expression and RA-promoted neurite outgrowt

143 rofascin (186 kDa) coimmunoprecipitated with doublecortin from detergent extracts of embryonic brain

144 Mutations in the gene that impair doublecortin function and cause severe brain formation d

145 C-terminal domains, which can be related to doublecortin function.

146 understand the role of individual domains in doublecortin function.

147 Although mutations in the human doublecortin gene (DCX) cause profound defects in cortic

148 Mutations in the doublecortin gene (DCX) in humans cause malformation of

149 Mutations in the X-linked doublecortin gene appear in many sporadic cases of doubl

150 ermal and progenitor markers such as nestin, doublecortin, GFAP, neurofilament, and vimentin.

151 show here by video microscopy that purified doublecortin has no effect on the growth rate of microtu

152 ntig also contains two brain-specific genes, doublecortin (HGMW-approved symbol DCX), responsible for

153 Furthermore, doublecortin immunoelectron microscopy was used to exami

154 SA-WD and CSA-CONT resulted in more immature doublecortin-immunopositive (+) neurons in the posterior

155 ubventricular zone (SVZ), migrating neuronal doublecortin immunoreactive cells and VEGF and bFGF expr

156 BrdU and doublecortin-immunoreactive (BrdU+/DCX+) cells were seen

157 cked with an antibody, enhanced migration of doublecortin-immunoreactive neurons in 1 d differentiate

158 using bromodeoxyuridine, Ki-67, nestin, and doublecortin immunostaining in the ischemic brain, and d

159 ane and could be important for a function of doublecortin in directing neuronal migration.

160 d progressively from dorsal-to-ventral, (ii) doublecortin in layer-2 calbindin-positive-patches disap

161 a raise the possibility that the function of doublecortin in neurons is to drive assembly and stabili

162 This study describes a novel activity of doublecortin in recognition of the FIGQY-phosphotyrosine

163 at express the early neuronal marker protein doublecortin in the subventricular zone of mouse brain.

164 genitors expressing the neuronal marker Dcx (Doublecortin) in the SVZ and the DG.

165 tional adult neurogenesis markers, Ki-67 and doublecortin, in the hippocampus was evaluated by immuno

166 How doublecortin influences microtubule dynamics, and thereb

167 Doublecortin is a cytoplasmic protein mutated in the neu

168 We further show that doublecortin is a direct gene target of REST, and that i

169 Doublecortin is a microtubule-associated protein produce

170 Doublecortin is a neuronal microtubule-stabilising prote

171 lation of the microtubule-associated protein Doublecortin is controlled by protein phosphatase 1 and

172 In cells, doublecortin is enriched at the distal ends of neuronal

173 Mutation analysis for LIS1 and doublecortin is essential in determining the etiology of

174 The molecular mechanism of action of doublecortin is only incompletely understood.

175 le cortex, but because LIS1 is autosomal and doublecortin is X-linked (on the X chromosome), the dise

176 ions in the X-chromosomal gene DCX, encoding doublecortin, is the main cause of classical lissencepha

177 Doublecortin kinase-1 (DCK1) is a newly described multid

178 t on the identification of a protein kinase, doublecortin kinase-2 (DCK2), with a domain (DC) highly

179 he density of bromodeoxyuridine-, NeuN-, and doublecortin-labeled cells was compared between chicks 2

180 lecortin was similar at all timepoints, with doublecortin-labeled profiles located throughout all for

181 In support of this, increased doublecortin labeling of immature neurons was detected i

182 However, doublecortin labeling was not detectable in any midbrain

183 Mutations in the X-linked gene doublecortin lead to "double cortex" syndrome (DC) in fe

184 We have characterized the expression of doublecortin-like (DCL), a microtubule-associated protei

185 Here, we report that the doublecortin-like kinase (Dclk) gene functions in a part

186 otubule-associated proteins (MAPs) including doublecortin-like kinase (DCLK), a protein that shares h

187 Doublecortin (DCX) and doublecortin-like kinase (DCLK), closely related family

188 A second locus, doublecortin-like kinase (Dclk), encodes a protein with

189 and/or its structurally conserved paralogue, doublecortin-like kinase 1 (Dclk1), show impaired Kif1a-

190 We show with that the phenotype of Dcx/Doublecortin-like kinase 1 deficiency is consistent with

191 Doublecortin-like kinase 1 protein (DCLK1) is a gastroin

192 , we targeted one of the closest homologues, doublecortin-like kinase 2 (Dclk2).

193 al., and Deuel et al., provide evidence that doublecortin-like kinase is essential for proper neuroge

194 is issue of Neuron, three related studies on doublecortin-like kinase, a microtubule-associated prote

195 n depletion of the putative stem cell marker doublecortin-like kinase-1 (DCLK1) in the crypts.

196 Using genetic lineage tracing, we show that Doublecortin-like kinase-1 (Dclk1) labels a rare populat

197 We discovered that doublecortin-like kinases (DCLKs), members of the double

198 1/Transforming-Acidic-Coiled-Coil, and ZYG-8/Doublecortin-Like-Kinase, both of which are required for

199 that other genetic loci or mosaicism at the doublecortin locus may be responsible for this diversity

200 Together with recent results showing that Doublecortin may play a role regulating the morphology o

201 euronal microtubule-associated protein (MAP) doublecortin, mediate the interaction between RP1 and mi

202 pal vascular endothelial growth factor mRNA, doublecortin mRNA, doublecortin protein, doublecortin-po

203 four groups: anterior biased/global DC with doublecortin mutation (16 of 30; 53%), anterior biased/g

204 dic DC patients had been found not to harbor doublecortin mutations and to determine whether clinical

205 their families, we found evidence for mosaic doublecortin mutations in 6 individuals.

206 To determine the incidence of doublecortin mutations in DC, we investigated a cohort o

207 Additionally, Doublecortin mutations in females lead to a more variabl

208 on deletions and point mutations, as well as Doublecortin mutations in males, lead to a very similar

209 adic double cortex patients show independent doublecortin mutations, at least one of them a de novo m

210 and XLIS are sporadic, representing de novo doublecortin mutations, we considered that some of these

211 istinguish between patients with and without doublecortin mutations.

212 gave rise to small numbers of immature, DCX (doublecortin)-negative neurons in the ventral forebrain,

213 e cells were grown as spheres that expressed doublecortin, nestin, and betaIII-tubulin, as well as th

214 n of neuronal cell markers (betaIII-tubulin, doublecortin, NeuN), the extent of dendritic arborizatio

215 ls that expressed markers of neurons (HuC/D, doublecortin), neural precursors (Sox10, nestin, Phox2b)

216 NA levels of four NeuroD downstream targets: doublecortin, Notch1, neurogenic differentiation 4, and

217 Doublecortin on the X-chromosome (DCX) is a neuronal mic

218 Doublecortin on X chromosome (DCX) is one of two major g

219 oradic patients with DC for mutations in the doublecortin open reading frame as assessed by single-st

220 effect on proliferation (Ki67), maturation (doublecortin) or survival (bromodeoxyuridine) of new adu

221 kers of neuronal and glial immaturity (Tuj1, doublecortin, or NG2).

222 racts of embryonic brain membranes, and this doublecortin-phospho-FIGQY neurofascin complex was disas

223 imer's brains showed increased expression of doublecortin, polysialylated nerve cell adhesion molecul

224 Importantly, the absolute number of immature doublecortin positive neuroblasts was significantly incr

225 that ketamine accelerates differentiation of doublecortin-positive adult hippocampal neural progenito

226 ed that p38 MAP kinase (p38) is expressed in doublecortin-positive adult NPCs.

227 ntate gyrus colocalized more frequently with doublecortin-positive and Ki67 proliferating neural prog

228 , we show that bromodeoxyuridine-labeled and doublecortin-positive cells from the SVZ colocalize with

229 risingly, young Arg-61 mice had more mitotic doublecortin-positive cells in the subgranular zone; mRN

230 001 significantly decreases the migration of doublecortin-positive cells that extend from the SVZ int

231 ith sedentary animals, the highest number of doublecortin-positive hippocampal cells was observed in

232 cell proliferation and ectopically localized doublecortin-positive immature neurons and radial glia-l

233 Our results show that doublecortin-positive immature neurons displayed increas

234 rons and dramatically increased apoptosis of doublecortin-positive neural progenitor cells in the sub

235 sis, as shown by a decrease in the number of doublecortin-positive neuroblasts (-28%), and mature, mi

236 oreactivity, but did not alter the number of doublecortin-positive neuroblasts at the end of the trea

237 NA, doublecortin mRNA, doublecortin protein, doublecortin-positive neuron counts and neurite length i

238 ed the formation of long and highly branched doublecortin-positive neurons in the subgranular zone of

239 adult TGF-beta1 mice had 60% fewer immature, doublecortin-positive, hippocampal neurons than wild-typ

240 lon binds to the microtubule binding protein doublecortin preventing its degradation.

241 Rare Sox2(+) cells produce rapidly cycling doublecortin(+) progenitors that, together with their po

242 elial growth factor mRNA, doublecortin mRNA, doublecortin protein, doublecortin-positive neuron count

243 the cell biology of the LIS1 protein and the Doublecortin protein, potentially interacting pathways n

244 amino terminus has similarity to that of the doublecortin protein, whose gene (DCX) has been implicat

245 further understanding of LIS1 protein and of Doublecortin protein.

246 regions are critical for the function of the Doublecortin protein.

247 ed by neuronogenic cells in the SVZ, such as doublecortin, PSA-NCAM, beta-tubulin, Dlx2, or GFAP.

248 ventricle stained for the neuroblast marker doublecortin revealed normal formation of chains of migr

249 These data reveal the structural basis for doublecortin's binding selectivity and provide insight i

250 evels of four hallmark NPC proteins (nestin, doublecortin, sex-determining homeobox 2, and glial fibr

251 Doublecortin specifically recognized the phospho-FIGQY t

252 We show that doublecortin-stabilised microtubules are substrates for

253 Doublecortin stabilizes microtubules and stimulates thei

254 However, doublecortin staining did show large parts of the caudal

255 igrating neurons marked by Ki67, nestin, and doublecortin, such as those in the subventricular zone a

256 lar to those of both CaM kinases (CaMKs) and doublecortin, the product of the gene mutated in X-linke

257 used a marker of neurogenesis and migration, doublecortin, to further characterize the response of th

258 f new neurons, assessed by the expression of doublecortin, to HVC.

259 The distribution of doublecortin was similar at all timepoints, with doublec

260 d the lesion expressed the neuroblast marker doublecortin, whereas human cells at the lesion border e

261 f these cells stained for betaIII-tubulin or doublecortin, which are molecules expressed by migrating

262 Mutations in the X-linked gene doublecortin, which encodes a protein with no dear struc

263 , a pattern that resembles the expression of doublecortin, which is implicated in neuronal migration.

264 Reducing the expression of stathmin or doublecortin with an antisense oligonucleotide inhibited

265 neurofascin provides the first connection of doublecortin with the plasma membrane and could be impor