ライフサイエンスコーパス: droplet

1 gles of less than 10 degrees for a 35 microL droplet.

2 measuring the fluorescence intensity of each droplet.

3 with the collision of a single sub-picoliter droplet.

4 ng promotes the formation of phase-separated droplets.

5 ntial electrochemical read-out of individual droplets.

6 some biological systems and synthetic active droplets.

7 variability, and content of rapidly passing droplets.

8 le duplexed reaction containing thousands of droplets.

9 expression, permitting deregulation of lipid droplets.

10 interconnected clusters of proteins and oil droplets.

11 radation and the dynamic regulation of lipid droplets.

12 fluctuations generated by only 5-10 passing droplets.

13 a condensation or demixing of proteins into droplets.

14 of drying lyotropic chromonic liquid crystal droplets.

15 urst spontaneously into thousands of smaller droplets.

16 ells, brown adipocytes, and artificial lipid droplets.

17 d 1.4 times slower than individually trapped droplets.

18 gy, a selective autophagy that targets lipid droplets.

19 roblasts through the quantification of lipid droplets.

20 or transcriptional activities but more lipid droplets.

21 transient protein complexes found in aqueous droplets.

22 astomers, shape-memory polymers, and aqueous droplets.

23 al arms that correspond to remarkably dilute droplets.

24 aps limited environment of superfluid helium droplets.

25 h a decreased accumulation of TAGs and lipid droplets.

26 reversibility in individual nanoliter-sized droplets.

27 ion of germ granules to coalesce into liquid droplets.

28 Blood droplets (35 microL) roll through the tubes at 15 degree

29 induced increased liver triglyceride and fat droplet accumulation.

30 The droplet adsorption onto the foam was governed by physiso

31 Aptamers-in-droplets affords a general approach for evolving microbe

32 rmine the incidence of presumed silicone oil droplets after intravitreal bevacizumab was prepared in

33 The extent of droplet aggregation and creaming was largely independent

34 ated that the compartmentalization of LYS in droplets also comprising gold nanoparticles provided enh

35 ults provide a direct link between the lipid droplet and proteasomal protein degradation and suggest

36 bilayer formed at the interface between each droplet and the hydrogel; each bilayer then incorporated

37 sults in three-dimensional motion inside the droplet and thus fast mixing.

38 d to cause a controllable deformation of the droplet and/or oscillation along the vortex axis.

39 ntify the beta-carotene concentration in oil droplets and determine the partitioning characteristics

40 d an association of nsP3 with cellular lipid droplets and examined the spatial relationships between

41 tial diameters of simulated petroleum liquid droplets and gas bubbles by 3.2-fold and 3.4-fold, respe

42 as-enriched spray with small, highly charged droplets and ions and directing it toward the MS inlet.

43 e antibodies are confined within the dextran droplets and locally stain the tissue below with a contr

44 thereby leading to the accumulation of lipid droplets and promoting tumor-associated macrophage diffe

45 We obtain the flow field inside the droplets and show that as the torus evolves, its cross-s

46 at the interface of the encapsulated aqueous droplets and the encasing hydrogel.

47 formation of these remarkable and ubiquitous droplets and the way in which such interactions dictate

48 lysosome, peroxisome, mitochondria and lipid droplet) and show how these relationships change over ti

49 dd reagents into droplets, remove fluid from droplets, and perform other necessary operations, each t

50 ecrease water solubility, stabilize emulsion droplets, and promote interdroplet adhesion.

51 es transmitted among ferrets via respiratory droplets, and the neuraminidase-sensitive variant killed

52 Pet10p binds early to nascent droplets, and their rate of appearance is decreased in p

53 acoustic vortex fields on a levitated water droplet are observed to cause a controllable deformation

54 Droplets are able to slide through a 4 mm (inner diamete

55 t LAF-1 and other in vitro and intracellular droplets are characterized by an effective mesh size of

56 ncapsulate lipid fraction, so that small oil droplets are entrapped within a dry matrix of roe protei

57 isted inlet ionization (DAII), where aqueous droplets are produced from airborne nanoparticles.

58 d analysis time by utilizing nanoliter-sized droplets as a replacement of wells.

59 se cells continue to cycle and utilize lipid droplets as a source of lipids.

60 particles are formed using superfluid helium droplets as the nanoreactors, which are strongly ferroma

61 med at the interface of generated extractant droplets as they entered into contact with the samples.

62 In order to engineer in-droplet assays, microfluidic devices must add reagents i

63 lly interacts with the endoplasmic reticulum droplet assembly factors seipin and Fit2 to maintain pro

64 his work introduces a new ionization method, droplet assisted inlet ionization (DAII), where aqueous

65 surface interactions become significant, and droplets assume distorted shapes.

66 pplied our analysis algorithm to multiplexed droplet-based digital PCR data sets in both EvaGreen and

67 Our new droplet-based method, sparse isoform sequencing (spISO-s

68 Droplet-based microfluidic systems that incorporate flow

69 consumption and high throughput capacity of droplet-based microfluidic systems.

70 d high-throughput methods for single-nucleus droplet-based sequencing (snDrop-seq) and single-cell tr

71 We describe a droplet-based system that enables 3' mRNA counting of te

72 We have created a 4 x 4 droplet bilayer array comprising light-activatable aqueo

73 r array comprising light-activatable aqueous droplet bio-pixels.

74 of magnitude smaller, the angles between the droplet branches are seen to exhibit remarkable universa

75 isplacement of surfactant molecules from the droplet bulk to the droplet-vapour interface.

76 e (HSL) and perilipin 1 (Plin1) in the lipid droplet by protein kinase A (PKA).

77 emulsion creamer that packs ("creams") assay droplets by draining away excess oil through microfabric

78 y as electric potential energy through water droplets by making alternate contacts with CYTOP and PTF

79 activating protein expression within single droplets, by using light-activated DNA to encode either

80 nnel ddPCR experiments in the case where the droplets can be grouped into four clusters.

81 The microarray of antibody droplets can be prespotted on a slide and stored, thus n

82 de glass microfluidic platform, in which oil droplets can be trapped as single droplets, or several d

83 Here we present an in-droplet cell concentrator that operates by first gradual

84 We suggest that "on-droplet" chemistry, similar to "on-water" chemistry, may

85 times increase in acceleration of a sliding droplet compared to a control tube depending on the incl

86 These data demonstrate the utility of droplet compartments for the quantitative characterizati

87 -propelled magnetic Pickering emulsion (MPE) droplets comprising particle-free fatty acid-stabilized

88 n, Iceland, reduced the size of liquid cloud droplets-consistent with expectations-but had no discern

89 The charged droplet consists of approximately 2400 water molecules,

90 We demonstrate that the droplets contained crystal-like lyotropic phases includi

91 droplet hydrodynamic system, where a 70 muL droplet containing a redox active species (ferrocyanide)

92 loped platform was first characterized using droplets containing different number of polystyrene (PS)

93 , formed inside the raindrops disperse micro-droplets containing soil bacteria during raindrop imping

94 t binds specifically to and is stabilized by droplets containing triacylglycerol (TG).

95 Each droplet contains a cell-free expression system and is co

96 Single-droplet control will be essential to power subsets of co

97 Biocompatible MDE enables in-droplet cultivation of different living species.

98 ted resolution for direct observation of oil droplet deposition, deformation, and detachment during s

99 prevented coalescence during 7days since the droplets diameter did not have significant change.

100 Here, Droplet Digital PCR (ddPCR) and qPCR platforms were dire

101 More sensitive droplet digital PCR experiments confirmed more SNVs as m

102 n allele frequencies of L1s were assessed by droplet digital PCR or Taqman genotyping.

103 In this work, we have optimized droplet digital PCR to broadly measure mutated alleles o

104 We performed multiplexed droplet digital polymerase chain reaction to detect spon

105 Liquid droplets dispensed on two juxtaposed branches are transp

106 oute to chemically trigger deaggregation and droplet dispersion.

107 drophobic precursor, which leads to repeated droplet division.

108 In this study, we analyzed the role of lipid droplets during the interaction of Cryptococcus neoforma

109 protein samples are compartmentalized inside droplets, each one acting as an isolated microreactor.

110 lled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that

111 The physics of droplet ejection under strong evaporative flow is descri

112 ed magnet allows selective capture of 96% of droplet-encapsulated superparamagnetic beads during 1:1

113 Finally, droplets encapsulating radiolabeled cancer cells allowed

114 phaltenes create elastic layers around water droplets enhancing stability of the emulsion matrix.

115 The infrared spectrum of the droplet ensemble reveals several signatures of polar, wa

116 When these droplets enter the mass spectrometer through a heated in

117 esolving an open problem in kinetics of fuel droplets evaporation.

118 Additionally, homogeneous immunoassays in droplets exhibited insulin detection limits of 9.3 nM or

119 Most droplet expansion took several hours and occurred in api

120 We employ bi-phase emulsion droplets fabricated from immiscible hydrocarbon and fluo

121 Ultimately, the nanoscale droplets flatten out to form layer-like molecular assemb

122 e fixed-location chambers (cdPCR) or aqueous droplets floating in oil (ddPCR).

123 The droplet flow is synchronized with synchrotron radiation

124 simple to implement into a variety of other droplet fluidics devices.

125 processors must generate, incubate, and sort droplets for continuous droplet screening, passively han

126 rks by partitioning a sample into individual droplets for which the majority contain only zero or one

127 s a quantitative technique in a miniaturized droplet format, which is shown to be as reliable as its

128 In contrast, palmitate-induced lipid droplet formation is significantly reduced in HepG2-SMS1

129 iple liquid flows, allowing the frequency of droplet formation or the average concentration of living

130 In the droplet formation zone, the eluted components are mixed

131 mic reticulum membrane morphology, and lipid droplet formation, but not on growth at elevated tempera

132 d in Opi1p being localized to sites of lipid droplet formation, coincident with increased synthesis o

133 changes in genes leading to increased lipid droplets formation in hepatocytes resulting in a downstr

134 Association of clay particles with droplets formed by liquid-liquid phase separation could

135 ripping mode at a predetermined dropsize and droplet frequency.

136 ion growth, necking and budding of offspring droplets from a bulk body are observed.

137 shedding and sweeping effectively remove the droplets from the solid surface in dropwise mode or thin

138 Analysis of pL droplets, generated at kHz frequencies and moving at hig

139 ible solution is to use an HPLC coupled to a droplet generating microfluidic device to sequentially e

140 and approximately equals to the width of the droplet-generating channel.

141 Toxoplasma infection, the induction of lipid droplet generation is conserved not only during infectio

142 ion of neutral lipids in intracellular lipid droplets has been associated with the formation and prog

143 Ga-BEP for offsetting the consumption of the droplets has been explored to realize long NWs with homo

144 on-collection electrochemistry in a rotating droplet hydrodynamic system, where a 70 muL droplet cont

145 complement, which in turn activates the next droplet in the sequence, akin to living polymerization.

146 ng polymer particles in 3D volumes and lipid droplets in adipose cells.

147 oleic acid increased the frequency of lipid droplets in both C. neoformans and macrophages.

148 ally reproduced dispersed plumes of fine oil droplets in Gulf of Mexico seawater and successfully rep

149 be generated by forming networks of aqueous droplets in lipid-containing oil.

150 ls are then trapped in water-in-oil emulsion droplets in the presence of primers and dNTPs, followed

151 d adiposity, and steatosis, with large lipid droplets in their hepatocytes.

152 ifferent reactants and levitating the merged droplet indefinitely.

153 digital microfluidics (DMF) addressing each droplet individually using 2D arrays of electrodes and u

154 molecules aggregate together and create nano-droplets inside the materials.

155 umbling and capable of diffusing through the droplet interior.

156 , extracting the analyte from aqueous sample droplets into the organic phase that contains the sensor

157 A subpicoliter aqueous aerosol droplet is captured in the optical trap and used as a sa

158 iling interaction (SO2)O...H(H2O) on a large droplet is mainly due to favorable exposure of H atoms o

159 mplexes of HMSA with (R1)(R2)NH on the water droplet is observed with a few femtoseconds, suggesting

160 and suggest that dynamic regulation of lipid droplets is a key aspect of some proteotoxic stress resp

161 Micron-scale droplets isolated by an immiscible liquid can provide mi

162 Droplets isolated from cells with a PET10 deletion stron

163 clouds are composed of randomly moving small droplets lacking any ordered structure.

164 the retentate was 3.6 mg L(-1), and all oil droplets larger than 140 nm were removed.

165 Lipid droplet (LD) accumulation is a hallmark of hypoxic cance

166 csin C, a fatty acid analogue, impairs lipid droplet (LD) biogenesis and ERAD, suggesting a role for

167 his study was designed to characterize lipid droplet (LD) formation in EC by manipulating pathways le

168 The lipid droplet (LD) fraction of milk has attracted special atte

169 Lipid droplet (LD) functions are regulated by a complement of

170 Lipid droplet (LD), a ubiquitous organelle in mammalian cells,

171 ABSTRACT: Because the lipid droplet (LD)-associated perilipin (PLIN) proteins promot

172 The length of the droplets (Ld) is highly correlated to the aqueous-phase

173 Plant cytosolic lipid droplets (LDs) are covered with a layer of phospholipids

174 During activation, HSCs lose their lipid droplets (LDs) containing triacylglycerols (TAGs), chole

175 h LC3 and SQSTM1 could colocalize with lipid droplets (LDs) following ethanol treatment.

176 ors are recruited to triglyceride-rich lipid droplets (LDs) in the liver by the GTPase ARF1, which is

177 Cytoplasmic lipid droplets (LDs) of neutral lipids (triacylglycerols [TAGs

178 CDCP1 depletes lipids from cytoplasmic lipid droplets (LDs) through reduced acyl-CoA production and i

179 tral lipid storage organelles known as lipid droplets (LDs).

180 close proximity to the apical side of lipid droplets (LDs).

181 (Ld - Lc) where Lc is the minimal obtainable droplet length and approximately equals to the width of

182 Concurrently, the droplet lifts from the substrate due to its incompressib

183 Our system, containing non-toxic nano-droplets loaded with iodine has high contrasting propert

184 iNAAT) platform utilizing a mobile phone and droplet magnetofluidics to deliver NAAT in a portable an

185 otential applications such as micro hand for droplet manipulation.

186 f analyte can easily be studied using single droplet mass spectrometry.

187 ity individually addressable electrochemical droplet microarray (eDMA).

188 chemical or living cell screenings using the droplet microarray platform with the sequential electroc

189 This work demonstrates a droplet microfluidic platform that has the potential to

190 e functionality and complexity of controlled droplet microfluidic systems can be positioned between d

191 rrays of electrodes and ultrahigh throughput droplet microfluidics focused on the generation of hundr

192 Droplet microfluidics is a relatively new and rapidly ev

193 Droplet microfluidics is among the most promising candid

194 radient-assisted purification of nuclei with droplet microfluidics to develop a highly scalable singl

195 ell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode

196 In this review we focus on 'controlled droplet microfluidics' - a portfolio of techniques that

197 cochemical environment has on both the lipid droplet microstructure and the lipid release patterns.

198 um was carried out to image the evolution of droplet morphology during reaction between Al and SiO2.

199 ying on two distinct mechanisms for coupling droplet morphology to enzyme activity (host-guest intera

200 y factors seipin and Fit2 to maintain proper droplet morphology.

201 s and finite element modeling of metal micro-droplet motion associated with metal additive manufactur

202 nt of absorbance within individual pL-volume droplets moving within segmented flows.

203 n China substantially increases column cloud droplet number concentration and liquid water path (LWP)

204 sposable thin-film electrode modified with a droplet of a gel-polymer electrolyte (GPE) was immersed

205 hnique based on single-cell encapsulation in droplets of a monodisperse microfluidic double water-in-

206 In this protocol, droplets of agarose gel containing different chemokines

207 we report on the crystallization of alloyed droplets of controlled micrometer dimensions comprising

208 Acoustically trapped droplets of oleic acid/sodium oleate mixtures in sodium

209 al or constitutional supercooling, resulting droplets often only display dendritic morphologies.

210 Moreover, regarding a liquid droplet on hydrophobic or superhydrophobic surfaces, an

211 ne-in-oil emulsions via coalescence of brine droplets on our dye-sensitized TiO2 surface upon visible

212 o smectic A in hybrid-aligned liquid crystal droplets on water substrates, using experimental, theore

213 Y-shaped junction, resulting in two daughter droplets, one of which containing all or most of the cel

214 r being separated in HPLC using microfluidic droplets online and represents an advance in the miniatu

215 alyte is sprayed uniformly through picoliter droplets onto the microcantilever surface; the micromete

216 e DNA and reagents are encapsulated within a droplet or microwell, allowing for analysis of precious

217 he intact complex in either the electrospray droplet or the gas phase produced c-type fragment ions.

218 which oil droplets can be trapped as single droplets, or several droplets per trap.

219 es short actin filaments into spindle-shaped droplets, or tactoids, with shape dynamics consistent wi

220 cleoprotein (RNP) granules are membrane-less droplet organelles that are thought to regulate posttran

221 ng both fluorescence spectroscopy and single droplet paper spray mass spectrometry.

222 an be trapped as single droplets, or several droplets per trap.

223 These data reveal a liquid droplet phase of actin, demixed from the surrounding sol

224 always observed to be a core of the original droplet phase surrounded by a shell of the added SOA.

225 tween AMY1 copy number (genotyped by digital droplet polymerase chain reaction) and obesity traits in

226 d as aerosols via the vapor-to-particle (or -droplet) process in a temperature gradient field.

227 nm, which determines the size scale at which droplet properties impact molecular diffusion and permea

228 Pet10p is a yeast lipid droplet protein of unknown function.

229 We previously reported that the lipid droplet protein perilipin 2 (PLIN2) modulates lipid home

230 Perilipin 2 (PLIN2) is a lipid-droplet protein that is up-regulated in alcoholic steato

231 Our results show that detection of lipid droplets provides a robust readout to screen for regulat

232 onance causes the increase of the condensate droplet radius without affecting the condensate fraction

233 ged as a function of the permeation rate, J, droplet radius, R, membrane permeance, k, water viscosit

234 microfluidic devices must add reagents into droplets, remove fluid from droplets, and perform other

235 are ameliorated by a reduction of the lipid-droplet-resident protein PLIN2.

236 nt densities (10(7) cells mL(-1)) and a high droplet resolution of 1 nL.

237 Periodic sampling by a droplet-robot demonstrates that the extent of fission is

238 the collection efficiency increases with the droplet rotation rate and decreases with the generator-c

239 mall capillary length liquids, whose sessile droplets sag by gravity.

240 , incubate, and sort droplets for continuous droplet screening, passively handling millions of drople

241 mulation to study the breakdown of spherical droplet shapes at small length scales.

242 Electrodes in each droplet simultaneously measured the light-driven proton-

243 Droplet single-cell RNA-sequencing (dscRNA-seq) has enab

244 rately changed, while directly measuring the droplet size allows the user to define the more meaningf

245 to define the more meaningful parameters of droplet size and generation frequency rather than flow r

246 tivation potential decreases with decreasing droplet size or increases with decreasing surface tensio

247 es to blue light resulted in decreased lipid droplet size, increased basal lipolytic rate and alterat

248 S)) and emulsifying (emulsion capacity (EC), droplet size, polydispersity (PDI), emulsifying activity

249 increasingly important with the increase of droplet size, reflecting a marked effect of the water su

250 c viscosity of water, and increases with the droplet size.

251 All emulsions had similar initial oil droplet sizes and were submitted to simulated gastrointe

252 cifically, early in the decay of the charged droplet SP(3+) ions favor an extended conformation, wher

253 apsulated superparamagnetic beads during 1:1 droplet splitting events at approximately 400 Hz.

254 ges, flow rates, and the width ratios of the droplet splitting microchannels.

255 ectrophoresis (nDEP), followed by asymmetric droplet splitting using a Y-shaped junction, resulting i

256 The hybrid-aligned nematic droplet spontaneously generates boojum defects.

257 These findings suggest that the droplet state can incubate tau and predispose the protei

258 ime (range, 2-8 months) despite the silicone droplets still being present on ophthalmoscopic examinat

259 the substrate drives a gradual reduction in droplet-substrate contact.

260 mass to be displaced toward the unsolidified droplet-substrate interface.

261 ransition can drive the formation of dynamic droplet substructures (vacuoles) with tunable lifetimes.

262 syn- and anti-CH2 C(CH3 )C(H)OO on the water droplet surface suggests that strongly hydrophobic subst

263 hat incorporate flowing streams of pL-volume droplets surrounded by a continuous and immiscible carri

264 ysis of secondary organic aerosol (SOA) on a droplet suspended in an aerosol optical tweezers (AOT).

265 ualization at 1873 K of 0% to 8% molten FeAl droplets suspended in a SiO2 enriched oxide medium was c

266 Here, we present an oil-in-water droplet system comprising an amphiphilic imine dissolved

267 s of thousands or even millions of picoliter droplets that cannot be individually addressed by their

268 The droplets that coalesced during digestion were hydrolyzed

269 which provides sufficient energy to heat-up droplets that pass through the capacitive gap.

270 Compared to a free falling droplet, the superhydrophobic tube reduced the accelerat

271 Furthermore, in lipid droplets, the phosphorylation of HSL and Plin1 and the l

272 Using the surface charge of the foam and oil droplets, the solution pH (5.6) for maximum separation e

273 Each droplet then becomes a reaction chamber for PCR, which t

274 Active strands on initiator droplets then displace one of the paired strands and thu

275 omote mixing of small liquid volumes (microL droplets) through a combination of coalescence and Lapla

276 he average concentration of living cells per droplet to be controlled and kept constant.

277 This is achieved at a macrosized droplet to create a liquid metal marble that comprises a

278 s by first gradually focusing cells inside a droplet to one side of the droplet using negative dielec

279 erature cycling to cause repeated breakup of droplets to higher-energy states.

280 eso- and microscale puncta (distinct protein droplets) to multilayered orthogonally phase-separated g

281 This source adds a new approach to charged droplet trajectory manipulation which, when combined wit

282 upted lipolysis without affecting ATGL lipid droplet translocation or ABHD5 interactions with perilip

283 ploys a flow-focusing geometry and generates droplets under constant pressure.

284 ng cells inside a droplet to one side of the droplet using negative dielectrophoresis (nDEP), followe

285 estigate the evolution of shrinking toroidal droplets using particle image velocimetry.

286 ctant molecules from the droplet bulk to the droplet-vapour interface.

287 As droplet volumes shrink to the scale of attoliters, howev

288 The 3D shape of the droplets was imaged as a function of the permeation rate

289 verted to cholesteryl esters stored in lipid droplets when ORP1L was bound to RIDalpha.

290 f compacted chromatin in phase-separated HP1 droplets, which are dissolved or formed by specific liga

291 ice aerosol generator providing monodisperse droplets, which are oxidized in a subsequent heat treatm

292 In this mode, DRILL removes larger droplets while accelerating the remainder of the ESI plu

293 gastric conditions leading to coalesced oil droplets, while Tween80 emulsions remained stable.

294 We demonstrate that single droplets with <100 pg of analyte can easily be studied u

295 In this step, droplets with cells expressing partner proteins that pro

296 et screening, passively handling millions of droplets with complete uniformity, especially during the

297 mical reactions to be initiated by colliding droplets with different reactants and levitating the mer

298 uate the performance of the microwave mixer, droplets with highly viscous fluid, 75% (w/w) glycerol s

299 o deliver multiple 100pico-liter (pL) volume droplets with pinpoint accuracy into a single diatom fru

300 rapidly solidifying, typically supercooled, droplets with surfaces, and to harvest benefit from it f