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1 has been reduced or eliminated with the new dye terminators.
4 merases, thus allowing cycle-sequencing with dye-terminators compatible with Taq DNA polymerase, as w
5 ak heights in terminator sequencing than the dye-terminators consisting of unsubstituted rhodamine dy
6 ort, OST has been optimized for fluorescent, dye-terminator cycle sequencing reactions to facilitate
7 f microreactors for small-volume PCR and for dye-terminator cycle-sequencing reaction, purification o
9 s were due to misincorporation of one of the dye-terminators during the primer extension reaction as
10 lative copy number information from standard dye-terminator electropherograms has been little explore
11 ethod was shown to effectively remove excess dye terminator from the CGE tract, but yielded lower pla
12 on, the fluorescence polarization of the two dye-terminators in the reaction mixture are analyzed dir
13 nce energy transfer, named template-directed dye-terminator incorporation (TDI) assay, has been devel
15 method, which we call the template-directed dye-terminator incorporation assay, is shown to be highl
17 tes labeled with different fluorescent dyes (dye terminators) is the most versatile method for automa
21 ese to reactions containing dRhodamine-based dye terminators produced the highest base-calling accura
22 ,000 oligos have been synthesized for use in dye terminator sequencing reactions, polymerase chain re
24 primer extension DNA fragments generated in dye-terminator sequencing cause background noise in fluo
26 manganese salt to the PE Applied Biosystems dye-terminator sequencing kits overcomes these limitatio
29 e-labeled primer is extended one base by the dye-terminator specific for the allele present on the te
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