ライフサイエンスコーパス: eIF

1 eIF-4E overexpression has been demonstrated in human tum

2 eIF-4E was over expressed in malignant cholangiocytes.

3 ore estimated the intracellular levels of 11 eIFs in logarithmically growing cells of Saccharomyces c

4 a subunit of eukaryotic initiation factor 2 (eIF-2alpha) and inhibit translation, we determined wheth

5 eukaryotic translation initiation factor 2 (eIF-2alpha) and the induction of apoptosis in lung cance

6 a subunit of eukaryotic initiation factor 2 (eIF-2alpha) by activated PKR, and, if provided prior to

7 a subunit of eukaryotic initiation factor 2 (eIF-2alpha) phosphorylation and initial suppression of v

8 a subunit of eukaryotic initiation factor 2 (eIF-2alpha) phosphorylation.

9 it of protein synthesis initiation factor 2 (eIF-2alpha) was elevated severalfold in DeltaE3L-infecte

10 eukaryotic translation initiation factor 2 (eIF-2alpha) was enhanced approximately 3-fold in polR ve

11 a subunit of eukaryotic initiation factor 2 (eIF-2alpha), as well as induce robust accumulation of ac

12 a subunit of eukaryotic initiation factor 2 (eIF-2alpha), was equally phosphorylated in EBV-positive

13 eukaryotic translation initiation factor 2 (eIF-2alpha).

14 eukaryotic translation initiation factor 2, (eIF-2 alpha).

15 ity and eukaryotic initiation factor 2alpha (eIF-2alpha) dephosphorylation, a finding consistent with

16 ryotic translation initiation factor 2alpha (eIF-2alpha).

17 creased eukaryotic initiation factor-2alpha (eIF-2alpha) phosphorylation.

18 KR) and eukaryotic initiation factor-2alpha (eIF-2alpha) was determined by Western blot.

19 n proteins, eukaryotic initiation factor 4E (eIF-4E) and 4E-binding protein (4E-BP1), a suppressor of

20 eukaryotic translation initiation factor 4E (eIF-4E).

21 on from the eukaryotic initiation factor-4E (eIF-4E) complex.

22 inds to the eukaryotic initiation factor-4E (eIF-4E), preventing formation of a functional eIF-4F com

23 tion of the eukaryotic initiation factor 5A (eIF-5A) is emerging as a crucial regulator in cancer, in

24 ion of eukaryotic initiation factor 2 alpha (eIF-2 alpha) is typically associated with stress respons

25 r, we found high phosphorylated eIF-2 alpha (eIF-2 alpha[P]) levels in nonstressed pancreata of mice.

26 lates translation initiation factor 2-alpha (eIF-2alpha), and inhibits translation.

27 Although eIF-4E regulates the recruitment of mRNA to ribosomes, a

28 nsistent with GADD34's role in assembling an eIF-2alpha phosphatase.

29 iso)4E from a resistant B. rapa line into an eIF(iso)4E knockout line of Arabidopsis thaliana proved

30 These results suggest that an eIF-2alpha-dependent translation inhibition mechanism is

31 e HCV IRES can form a binary complex with an eIF-free 40S ribosomal subunit.

32 ng complexes, initiation factor (eIF) 4F and eIF(iso)4F, as measured by nitrocellulose binding and fl

33 pha, that correlated with PKR activation and eIF-2alpha phosphorylation.

34 eIF4E and eIF(iso)4E from different plant species were shown previ

35 or eIF(iso)4F, and the subunits of eIF4F and eIF(iso)4F cross-link to STNV-1 TED, providing additiona

36 psis HSP21 and AMV RNA 4 used both eIF4F and eIF(iso)4F equally well.

37 activating the C. elegans eIF-4G homolog and eIF-2 subunits results in developmental arrest accompani

38 gulatory proteins 4E-BP1, p70 S6 kinase, and eIF-4E, thus providing a mechanism for the modulation of

39 of four genes--staufen, pumilio, oskar, and eIF-5C--yield defective memory.

40 In correlation, phosphorylation of PKR and eIF-2alpha was suppressed in cells expressing the VP35 p

41 Increased phosphorylation of PKR and eIF-2alpha were also observed in active IBD tissues.

42 osphosulfate-kinase, cysteine proteinase and eIF(4G), thus confirming the involvement of ROS scavengi

43 ion rapalogs and is mediated by a TORC1- and eIF-4E-dependent mechanism ultimately signaling to RAF.

44 eIF4F was essential for scanning, and eIFs 4A and 4B were insufficient to promote this process

45 than for the known eIF4G ortholog (known as eIF-4G or eIF4G), suggesting that Ofs substitutes for th

46 P1 being a component of the GADD34-assembled eIF-2 alpha phosphatase.

47 ion of GADD34 in assembling an ER-associated eIF-2 alpha phosphatase that regulates protein translati

48 on unstructured 5'-UTRs was enhanced by ATP, eIFs 4A and 4B, and the central domain of the eIF4G subu

49 Conversely, blocking eIF-4E function by expression of antisense RNA, or overe

50 he translational regulators p70 S6k, 4E-BP1, eIF-2B, and eEF2.

51 using sequestering of eIF-4E, a TORC1/4E-BP1/eIF-4E-mediated mechanism of ERK activation could explai

52 egulatory role in the inhibition of eIF2B by eIF (alphaP).

53 0S subunits, which can be mediated either by eIFs 2/1/1A or by Ligatin following ABCE1-dependent or -

54 Its activity is enhanced by eIFs 3j, 1, and 1A.

55 Fs) using yeast strains in which chromosomal eIF genes have been placed under the control of the tetO

56 nslational level by decreasing PKR-dependent eIF-2alpha phosphorylation.

57 o form a large complex that dephosphorylates eIF-2alpha and thereby prevents translation shutoff medi

58 lecular-weight complex that dephosphorylates eIF-2alpha.

59 ne phosphatase (PP1), which dephosphorylates eIF-2 alpha during cellular stresses.

60 eport that in the absence of eIF1 and DHX29, eIFs 4A, 4B and 4G promote efficient bypassing of stable

61 In contrast, inactivating the C. elegans eIF-4G homolog and eIF-2 subunits results in development

62 In isolation, pUL38 overexpression elevated eIF-2alpha phosphorylation, induced ATF4 accumulation, l

63 For example, eIF-4E is usually increased in bronchioloalveolar carcin

64 ignancy will improve the capacity to exploit eIF-4E as a therapeutic target and as a marker for human

65 unction of the translation initiation factor eIF-2.

66 phorylation of translation initiation factor eIF-2alpha seen following plasmid DNA transfection were

67 the eukaryotic translation initiation factor eIF-2alpha, the activation of RNase L, and the shutoff o

68 ylation of the translation initiation factor eIF-2alpha.

69 ylation of the translation initiation factor eIF-2alpha.

70 ation of protein synthesis initiation factor eIF-2alpha.

71 ylation of the translation initiation factor eIF-4E and inhibited host translation similarly under bo

72 lations of the translation initiation factor eIF-4E on S209 and of its inhibitory binding protein 4E-

73 ciation of the translation initiation factor eIF-4E with its binding protein 4E-BP1, an inhibitor of

74 adation of the translation initiation factor eIF-4G was very similar for both the WT and the double m

75 presses expression of the translation factor eIF-4E and the glutamate receptor subunit GluRIIA.

76 gulates expression of the translation factor eIF-4E at the NMJ, and Pum binds selectively to the 3'UT

77 calculation of an electronic impact factor (eIF) would be an objective, quantitative way to measure

78 Eukaryotic translation initiation factor (eIF) 1 is a central mediator of start codon recognition.

79 Eukaryotic initiation factor (eIF) 1 is a small protein (12 kDa) governing fidelity in

80 Eukaryotic initiation factor (eIF) 1 maintains the fidelity of initiation codon select

81 he eukaryotic translation initiation factor (eIF) 2 (a G protein) functions in its GTP-bound state to

82 ation factors, eukaryotic initiation factor (eIF) 2 and eIF3, to form preinitiation 48S ribosomal com

83 occur, namely eukaryotic initiation factor (eIF) 2 and eIF3.

84 t-tRNAi(Met)), eukaryotic initiation factor (eIF) 2, and guanosine triphosphate form a ternary comple

85 cterization of eukaryotic initiation factor (eIF) 2A, a translation initiation factor that binds Met-

86 th eukaryotic translation initiation factor (eIF) 2alpha phosphorylation and eIF4F complex dysfunctio

87 stress-induced eukaryotic initiation factor (eIF) 2alpha phosphorylation and reduced the concentratio

88 (PP1), and the eukaryotic initiation factor (eIF) 2alpha.

89 somal subunit, eukaryotic initiation factor (eIF) 3 and the eIF2/GTP/Met-tRNA(i)(Met) ternary complex

90 l 40S subunit, eukaryotic initiation factor (eIF) 3 and the ternary eIF2/Met-tRNA(i)(Met)/GTP complex

91 NA is bound to eukaryotic initiation factor (eIF) 3, eIF1, eIF1A, and an eIF2/GTP/Met-tRNAi(Met) tern

92 of eukaryotic translation initiation factor (eIF) 3, little is known on the molecular level.

93 Eukaryotic initiation factor (eIF) 3j is a subunit of eIF3 that binds to the mRNA entr

94 Eukaryotic initiation factor (eIF) 4A is a DEAD-box helicase that stimulates translati

95 Eukaryotic initiation factor (eIF) 4B is known to interact with multiple initiation fa

96 he eukaryotic translation initiation factor (eIF) 4B promotes the RNA-dependent ATP hydrolysis activi

97 he eukaryotic translation initiation factor (eIF) 4B promotes the RNA-dependent ATP hydrolysis activi

98 of eukaryotic translation initiation factor (eIF) 4E and eIF2alpha within 30 and 10 min, respectively

99 phorylates the eukaryotic initiation factor (eIF) 4E and the eIF4G components of eIF4F.

100 phorylation of eukaryotic initiation factor (eIF) 4E at Ser-209 in the C-terminal loop of the protein

101 0 S6 kinase or eukaryotic initiation factor (eIF) 4E pathways downstream of the mammalian target of r

102 amycin and MYC-eukaryotic initiation factor (eIF) 4E pathways, are predicted to be particularly sensi

103 he eukaryotic translation initiation factor (eIF) 4E, is regulated by modulating both its phosphoryla

104 nal repressor, eukaryotic initiation factor (eIF) 4E-binding protein (BP)-1, 4E-BP1, fivefold.

105 ylation of the eukaryotic initiation factor (eIF) 4E-binding protein 1 (4E-BP1) and increased binding

106 he eukaryotic translation initiation factor (eIF) 4E-binding protein 1 (4E-BP1), the two downstream e

107 Eukaryotic initiation factor (eIF) 4E-binding protein-1 (4E-BP1) and S6 kinase-1 (S6K1

108 phosphorylates eukaryotic initiation factor (eIF) 4E.

109 ic cap-binding complexes, initiation factor (eIF) 4F and eIF(iso)4F, as measured by nitrocellulose bi

110 Eukaryotic initiation factor (eIF) 4F binding to mRNA is the first committed step in c

111 rm eukaryotic translation initiation factor (eIF) 4F binds tightly to the mRNA internal ribosome entr

112 he eukaryotic translation initiation factor (eIF) 4F complex to regulate the sensitization of nocicep

113 he assembly of eukaryotic initiation factor (eIF) 4F complexes consisting of eIF4E, eIF4G, eIF4A1, an

114 ratus, such as eukaryotic initiation factor (eIF) 4G (type 2), 40S ribosomal subunits (types 3 and 4)

115 eractions with eukaryotic initiation factor (eIF) 4G and eIF4B.

116 n functions as eukaryotic initiation factor (eIF) 4G binding partner or eIF4E(S209) kinase.

117 irectly to the eukaryotic initiation factor (eIF) 4G component of the eIF4F cap-binding complex.

118 petes with the eukaryotic initiation factor (eIF) 4G for TOP mRNA binding.

119 he eukaryotic translation initiation factor (eIF) 4G is a scaffold protein that organizes the assembl

120 he eukaryotic translation initiation factor (eIF) 4G is required during protein synthesis to promote

121 g protein with eukaryotic initiation factor (eIF) 4G, bridging the 5' terminal cap structure.

122 rovided by the eukaryotic initiation factor (eIF) 4G/PABP/poly(A) tail interaction is achieved instea

123 nd eukaryotic translation initiation factor (eIF) 4GII.

124 omain (CTD) of eukaryotic initiation factor (eIF) 5 interacts with eIF1, eIF2beta, and eIF3c, thereby

125 Eukaryotic translation initiation factor (eIF) 5 is crucial for the assembly of the eukaryotic pre

126 s: eukaryotic translation initiation factor (eIF) 5A.

127 also binds the eukaryotic initiation factor (eIF) complex 4F and its associated proteins.

128 Eukaryotic initiation factor (eIF) eIF1 maintains the fidelity of initiation codon sel

129 rging onto the eukaryotic initiation factor (eIF) eIF4F complex.

130 of eukaryotic translation initiation factor (eIF) interactions in ribosomal pre-initiation complexes

131 ally conserved eukaryotic initiation factor (eIF), eIF1A, plays multiple roles throughout initiation:

132 ation state of eukaryotic initiation factor (eIF)-2 alpha, which is induced by kinases like protein k

133 subunit of the eukaryotic initiation factor (eIF)-2 complex, resulting in a shut-off of general trans

134 F)-1alpha1 and eucaryotic initiation factor (eIF)-4E remained unchanged.

135 ed deletion of eukaryotic initiation factor (eIF)-4E, a rate-limiting translational initiation factor

136 ylation of the eukaryotic initiation factor (eIF)-4E.

137 plex (TC) with eukaryotic initiation factor (eIF)2-GTP scans the mRNA leader for an AUG codon in favo

138 phorylation of eukaryotic initiation factor (eIF)2alpha in response to arsenite.

139 nd eukaryotic translation initiation factor (eIF)3 enable 43S preinitiation complexes containing eIF3

140 eractions with eukaryotic initiation factor (eIF)4A and eIF4G, which are mediated via the two tandem

141 ylation of the eukaryotic initiation factor (eIF)4E-binding protein-1 (4E-BP1), enhanced association

142 of eukaryotic translation initiation factor (eIF)4E-bound mRNA.

143 nd eukaryotic translation initiation factor (eIF)4E-bound mRNAs, unlike in mammalian cells, where NMD

144 n vitro; (iii) eukaryotic initiation factor (eIF)4F binds specifically with high affinity to IRE-RNA;

145 ction with the eukaryotic initiation factor (eIF)4G and recruitment of activated RSK1 to 5' cap mRNA.

146 nds to and phosphorylates initiation factor (eIF)4G, which inhibits association of eIF4E with m(7)GTP

147 k reveals that eukaryotic initiation factor (eIF)5B actually accelerates the rate of ribosomal subuni

148 of eukaryotic translation initiation factor (eIF-2alpha).

149 on regulators (eg, CCAAT box binding factor, eIF-1AY).

150 ng phosphorylation of the initiation factor, eIF-2alpha.

151 itiator transfer RNA and initiation factors (eIF) 2, 3, 1 and 1A, attach to the 5'-terminal region of

152 ction between eukaryotic initiation factors (eIF) 4E and 4G, attenuates fear memory consolidation but

153 ryotic protein synthesis initiation factors (eIF) eIF4G and eIF4E, were up-regulated in mammary tumor

154 observed the translation initiation factors (eIF)4E and eIF4G in P-bodies at a low level during gluco

155 l eukaryotic translation initiation factors (eIFs) 1 and 1A at their C termini with tetramethyl rhoda

156 tion requires eukaryotic initiation factors (eIFs) 1, 1A, 2, 3, 4A, 4B and 4G, and the poly(C) bindin

157 ndicated that eukaryotic initiation factors (eIFs) 1, 2 and 5 play key roles in these events.

158 omal subunit, eukaryotic initiation factors (eIFs) and initiator tRNA scans mRNA to find an appropria

159 ires multiple eukaryotic initiation factors (eIFs) and ribosome.

160 eukaryotic translational initiation factors (eIFs) has been shown to be an important means of regulat

161 y eukaryotic translation initiation factors (eIFs) in conjunction with the 40 S ribosomal subunit and

162 activities of eukaryotic initiation factors (eIFs) is critical to our understanding of the mechanisms

163 subset of the eukaryotic initiation factors (eIFs) needed for canonical initiation on cellular mRNAs.

164 y eukaryotic translation initiation factors (eIFs) using yeast strains in which chromosomal eIF genes

165 sted that two eukaryotic initiation factors (eIFs), i.e. eIF4G and eIF3, were necessary.

166 involves many eukaryotic initiation factors (eIFs).

167 n of specific eukaryotic initiation factors (eIFs).

168 lves multiple eukaryotic initiation factors (eIFs).

169 requires at least seven initiation factors (eIFs).

170 s a process facilitated by numerous factors (eIFs), aimed to form a "scanning" mechanism toward the i

171 n antibody microarray was used to screen for eIF-4E-dependent proteins expressed during hypoxia.

172 Among the four eIF-2 alpha kinases present in mammals, PERK is most hig

173 hosphorylation of 4E-BP1 dissociates it from eIF-4E, relieving the translation block.

174 the inhibition of translation resulting from eIF-2alpha phosphorylation.

175 IF-4E), preventing formation of a functional eIF-4F complex essential for cap-dependent initiation of

176 cell cycle progression in the face of global eIF-4E-mediated translation inhibition.

177 rest as a result of the inhibition of global eIF-4E-mediated translation.

178 cinoma cells with Myb34.5 results in greater eIF-2alpha dephosphorylation and viral replication compa

179 ting that it may be responsible for the high eIF-2 alpha[P] levels found therein.

180 f their eukaryotic homologs, eukaryotic IFs (eIFs) eIF1A and eIF5B, has only recently become evident.

181 c evidence that the hypusine modification in eIF-5A is crucial for homeostasis in mammals.

182 Reduction in eIF-4E expression by siRNA decreased tumor cell resistan

183 weak context depends on specific residues in eIFs 1, 1A, and 2beta that also impede selection of non-

184 Herein, we show that increased eIF expression in tumor extracts of mice after daidzein

185 compared with lower eukaryotes and indicate eIF-5A2 as a valuable and safe target for therapeutic in

186 the unfolded protein response (UPR)-induced eIF-2alpha phosphorylation to protect against endoplasmi

187 lates protein homeostasis through inhibiting eIF-2alpha kinases including double-stranded RNA-depende

188 nse RNA, or overexpression of the inhibitory eIF-4E binding proteins (4E-BPs), suppresses cellular tr

189 Translational initiation involving eIF-4E and its inhibitory binding protein 4E-BP1 appear

190 includes BiP and XBP-1, and another that is eIF-2alpha kinase-dependent, which includes ATF4 and GAD

191 monstrate that the cancer-associated isoform eIF-5A2 is dispensable for normal development and viabil

192 se as well as for the cancer-related isoform eIF-5A2.

193 nitiation factor 4E (eIF4E) and its isoform, eIF(iso)4E.

194 nitiation factor 4E/MAPK-interacting kinase (eIF-4E/MNK) pathway.

195 Many eIFs have been found to have aberrant expression in huma

196 y to the atypical HEAT repeats found in many eIFs.

197 gamma(1)34.5 protein is capable of mediating eIF-2alpha dephosphorylation without any other viral pro

198 Our results implicate activation of eIF-4E as a key event in oncogenic transformation by pho

199 Addition of eIF(iso)4G and eIF4B that had also been phosphorylated i

200 th purvalanol A inhibited the association of eIF-4E with eIF-4G in PTX treated cells.

201 f 4E-BP1 by PTX increased the association of eIF-4E with eIF-4G, whereas cotreatment with purvalanol

202 Augmentation of eIF-2a phosphorylation minimizes motoneuronal injury in

203 ates synaptic function via direct control of eIF-4E expression.

204 is, TuMV appears to be able to use copies of eIF(iso)4E at two loci.

205 Transformation of different copies of eIF(iso)4E from a resistant B. rapa line into an eIF(iso

206 We identified three copies of eIF(iso)4E in a number of Brassica rapa lines.

207 ability of TuMV to access multiple copies of eIF(iso)4E in B. rapa and the broad spectrum of the resi

208 cose stimulated a rapid dephosphorylation of eIF-2 alpha.

209 oduct that promotes the dephosphorylation of eIF-2alpha) that is under control of the E2F-responsive

210 Furthermore, ectopic expression of eIF-4E blunted pp242-induced ERK phosphorylation.

211 ma of the lung, melanoma), the expression of eIF-4E is barely detectable.

212 eplacement of the truncated viral homolog of eIF-2alpha (FV3-DeltavIF-2alpha) or the 18K IE gene (FV3

213 lysis indicates that hyperphosphorylation of eIF-2alpha caused by HSV is greater in PKR+/+ cells than

214 lting in phosphorylation and inactivation of eIF-2alpha, an essential factor in protein translation.

215 in mouse 3T6 cells, which is independent of eIF-2alpha dephosphorylation.

216 on of C114 protein inhibits the induction of eIF-2alpha phosphorylation following poly(I.C) treatment

217 t that PTX-increases the functional level of eIF-4E by promoting the hyperphosphorylation and release

218 Mutation of eIF-2alpha to prevent phosphorylation also impaired IFN-

219 ibitor rapamycin and/or by overexpression of eIF-4E binding protein 1 (4E-BP1), which inhibits transl

220 Overexpression of eIF-4E in experimental models dramatically alters cellul

221 Phosphorylation of eIF(iso)4E has effects on m(7)G cap-binding affinity sim

222 neurons showed persistent phosphorylation of eIF-2a across hypoxia/reoxygenation, without activations

223 The mutant blocks the phosphorylation of eIF-2alpha but does not restore the virulence phenotype

224 RF63 mutant had increased phosphorylation of eIF-2alpha compared with cells infected with parental vi

225 Phosphorylation of eIF-2alpha results in attenuation of protein translation

226 of protein synthesis, the phosphorylation of eIF-2alpha, and activation of RNase L.

227 viral proteins inhibited phosphorylation of eIF-2alpha.

228 alter the expression and phosphorylation of eIF-4E.

229 Protection of eIF-2alpha phosphorylation with systemically administere

230 hese observations suggest that regulation of eIF-2alpha phosphorylation by the gamma(1)34.5 protein i

231 A deeper understanding of the role of eIF-4E in regulating the translation of the diverse gene

232 Models to explain the roles of eIF(iso)4E during virus infection are presented.

233 nt than rapamycin in causing sequestering of eIF-4E, a TORC1/4E-BP1/eIF-4E-mediated mechanism of ERK

234 ted by HSV infection parallels the status of eIF-2alpha phosphorylation.

235 4E-binding protein (4E-BP1), a suppressor of eIF-4E in the dephosphorylated state.

236 J, and Pum binds selectively to the 3'UTR of eIF-4E mRNA.

237 y confirmed using a high affinity variant of eIF-4E to capture 5'-methylguanosine-capped RNA followed

238 Here we evaluate the abundance of eIFs and their pre-initiation intermediate complexes in

239 The apparent rate control effects of eIFs observed in standard cell-free extract experiments,

240 ring the expression level or the function of eIFs may influence the synthesis of some proteins and co

241 ranslation in eukaryotes requires a suite of eIFs that include the cap-binding complex, eIF4F.

242 om the gamma(1)34.5 protein has no effect on eIF-2alpha dephosphorylation, further truncations up to

243 These findings imply that proto-oncogenic eIFs likely exert their tumorigenic function by regulati

244 believed to occur on a single protein only (eIF-5A).

245 tion with the cap binding subunits (eIF4E or eIF(iso)4E) of eIF4F or eIF(iso)4F.

246 tion is reversed by the addition of eIF4F or eIF(iso)4F, and the subunits of eIF4F and eIF(iso)4F cro

247 g subunits (eIF4E or eIF(iso)4E) of eIF4F or eIF(iso)4F.

248 virus did not significantly increase PKR or eIF-2alpha phosphorylation in either PKR-sufficient or -

249 of rate control over translation than other eIFs.

250 of 43S complexes is mediated by three other eIFs, 4F, 4A, and 4B, which cooperatively unwind the cap

251 We found that c-Myc overrides eIF-4E-induced cellular senescence, whereas eIF-4E antag

252 redundancy between eIF4E and the paralogous eIF(iso)4E in resistant peas.

253 e(s) shared by other cellular stresses (PERK/eIF-2alpha phosphorylation-dependent).

254 However, we found high phosphorylated eIF-2 alpha (eIF-2 alpha[P]) levels in nonstressed pancr

255 whereas a critical balance of phosphorylated eIF-2a should minimize motoneuronal injury in obstructiv

256 While phosphorylated eIF-2alpha was undetectable in uninfected cells or cells

257 ng cell defense responses by phosphorylating eIF-2alpha, thus suppressing RNA translation.

258 nistically, we found that IL-32 used the PKR-eIF-2alpha as well as the MxA antiviral pathways.

259 ed an absence of GADD34 induction, prolonged eIF-2 alpha phosphorylation, delayed protein synthesis r

260 necessary, it was not sufficient to promote eIF-2 alpha dephosphorylation in cells.

261 that recombinant wheat cap-binding protein, eIF(iso)4E, produced from E. coli can be phosphorylated

262 ontribution of the mRNA cap-binding protein, eIF-4E, to malignant transformation and progression has

263 ine repeat-rich proteins, which are putative eIF-5A targets, revealed that these proteins are organiz

264 iated initiation under conditions of reduced eIF-4F complex formation and Akt activity.

265 ctivates a translation initiation regulator, eIF-4E-binding protein 1 (4EBP), asymmetrically and trig

266 ds to better activation of PKR and resultant eIF-2alpha phosphorylation.

267 GADD34 expression also reversed eIF-2 alpha phosphorylation induced by okadaic acid but

268 n of human GADD34 in cultured cells reversed eIF-2 alpha phosphorylation induced by thapsigargin and

269 We now report that these seven eIFs are not sufficient for efficient 48S complex format

270 UPR, including PERK, the ER stress-specific eIF-2alpha kinase; ATF4, an ER stress-induced transcript

271 eral serine/threonine kinases (PCTK3, STK25, eIF-2A, PIM-3, PKA C-alpha, and PKN2).

272 complexes comprising 40S ribosomal subunits, eIFs 3, 2, 1, and 1A, and tRNA(Met)(i) attach to the 5'-

273 y regulates cap-dependent protein synthesis, eIF-4E contributes to malignancy by selectively enabling

274 These results indicate that eIF-2alpha dephosphorylation mediated by the gamma(1)34.

275 ilities, whereas the other arm activates the eIF-2alpha (alpha subunit of eukaryotic initiation facto

276 which is paradoxically translated during the eIF-2 alpha-mediated translational block, is required fo

277 ggesting that the mutual interactions of the eIF segments within the PIC prime the ribosome for initi

278 l mechanism based on the mis-splicing of the eIF(iso)4E allele in some TuMV-resistant B. rapa var. pe

279 One SNP in the regulatory region of the eIF-2alpha gene revealed A/G alleles.

280 WT SV5 was a poor activator of the eIF-2alpha kinase protein kinase R (PKR).

281 sponse targets like BiP are sensitive to the eIF-2 alpha-mediated block in translation.

282 gative feedback regulatory loop in which the eIF-2 alpha-controlled inhibition of protein translation

283 ation pathway is distributed over all of the eIFs, whereby rate control (the magnitude of their respe

284 osphorylation of mammalian eIF4E even though eIF(iso)4E lacks an amino acid that can be phosphorylate

285 ranslational initiation of this mRNA through eIF-4E- and 5' cap-independent internal ribosomal entry.

286 ving Akt1/mTOR complex 1 signaling (and thus eIF-4F-mediated translation initiation) from suppression

287 , and resistance to interferon is coupled to eIF-2alpha dephosphorylation.

288 the unfolded protein response (UPR), lead to eIF-2alpha phosphorylation and increased expression of C

289 l interfering double-stranded RNA (siRNA) to eIF-4E decreased anchorage-independent growth of maligna

290 w that the initiation factor of translation (eIF-4E), a downstream effector of mTOR, has oncogenic ef

291 The in vitro phosphorylation site for wheat eIF(iso)4E was identified as Ser-207.

292 ever, was unfavorable (negative) except when eIF(iso)4E was phosphorylated and interacting with eIF(i

293 nding affinity was reduced 1.2-2.6-fold when eIF(iso)4E was phosphorylated.

294 eIF-4E-induced cellular senescence, whereas eIF-4E antagonizes c-Myc-dependent apoptosis in vivo.

295 l A inhibited the association of eIF-4E with eIF-4G in PTX treated cells.

296 PTX increased the association of eIF-4E with eIF-4G, whereas cotreatment with purvalanol A inhibited

297 hough both NAFL and NASH are associated with eIF-2alpha phosphorylation, there is a failure to activa

298 A MB 231, which reduced its association with eIF-4E, but did not alter the expression and phosphoryla

299 o)4E was phosphorylated and interacting with eIF(iso)4G.

300 isiae forms a multifactor complex (MFC) with eIFs 1, 2, 5 and Met-tRNA(i)(Met).