ライフサイエンスコーパス: ecotropic

1 Ecotropic, amphotropic (4070A), and amphotropic-mink cel

2 This phenomenon is observed with both ecotropic and amphotropic MuLV Env.

3 We have examined the infectious entry of ecotropic and amphotropic MuLVs and found that they were

4 dicate that the infectious processes of both ecotropic and amphotropic MuLVs were arrested rather tha

5 nt lines were resistant to infection by both ecotropic and amphotropic murine viruses, as well as by

6 ctor (SCF) on beta-galactosidase-transducing ecotropic and amphotropic retroviral vector particles as

7 n these HSC were analyzed simultaneously for ecotropic and amphotropic retrovirus transduction, 11 of

8 n these HSC were analyzed simultaneously for ecotropic and amphotropic retrovirus transduction, ecotr

9 Ecotropic and amphotropic viruses differ in their abilit

10 cause they bear numerous similarities to the ecotropic and MCF viruses of the Rauscher MuLV complex b

11 These MuLVs comprised ecotropic and mink lung cell focus-forming (MCF) virus c

12 gion exhibiting maximum homology between the ecotropic and polytropic env sequences and maximum stabi

13 d vectors, HERE and HERA, which code for the ecotropic and the amphotropic envelopes, respectively.

14 ag is relatively poorly conserved even among ecotropic and xenotropic MLVs, it was also fully suffici

15 nt demonstrated that endogenous BALB/c mouse ecotropic and xenotropic MuLVs are activated by these tr

16 It is conceivable that this novel ecotropic-based conjugate retrovirus vector could also p

17 undly reduced the efficiency of infection by ecotropic, but not amphotropic, MLV reporters.

18 ree medium at very low frequency, as did the ecotropic control.

19 Stk is dependent on cysteine residues in the ecotropic domain of gp55 and the extracellular domain of

20 B6C3F1 hybrid mice carry ecotropic endogenous proviral sequences that may be acti

21 Furthermore, in addition to the single ecotropic endogenous retrovirus (eERV) located on chromo

22 bone, introduction of the first 17 aa of the ecotropic Env protein significantly increased the titer

23 Bindings of ecotropic envelope glycoprotein gp7O to the accessible r

24 y the retroviruses entering the cell via the ecotropic envelope pathway.

25 We inserted a His6 peptide into an ecotropic envelope protein (Env) by replacing part of it

26 utation was conditional on expression of the ecotropic envelope receptor murine cationic amino acid t

27 n the CNS, which was efficiently mediated by ecotropic Envs (CasBrE and Friend), but not 4070A amphot

28 ulated mice was markedly enhanced, while the ecotropic F-MuLV load was unchanged.

29 induced in cats by intradermal injection of ecotropic feline leukemia virus (FeLV), subgroup A, plas

30 are known to arise via recombination between ecotropic FeLV-A and endogenous FeLV (enFeLV) env elemen

31 r the last nucleotide of the env gene in the ecotropic FeLV-A Rickard (FRA) provirus.

32 opic viral envelope and the soluble RBD from ecotropic Friend MLV was supplied to the culture medium.

33 g mice infected with defined mixtures of the ecotropic Friend murine leukemia virus (F-MuLV) and diff

34 ructural elements with known elements of the ecotropic Friend murine leukemia virus RBD monomer.

35 n suggests an orientation for the monomer of ecotropic Friend murine leukemia virus RBD.

36 A variant ecotropic Friend murine leukemia virus, F-S MLV, is capa

37 ruses, but not previously reported for mouse ecotropic gammaretroviruses.

38 Murine retroviruses containing ecotropic glycoproteins do not infect human cells since

39 s do not express the receptor that binds the ecotropic glycoproteins.

40 ading frame (ORF2) of both the defective and ecotropic helper virus components of LP-BM5.

41 tantly, lysis of target cells expressing BM5 ecotropic helper, and/or defective viral gag, demonstrat

42 and interference studies revealed a strictly ecotropic host range and the use of a receptor distinct

43 FeLIX provided in trans, but it retained an ecotropic host range even in the presence of FeLIX.

44 Five of these chimeric viruses showed an ecotropic host range, and six exhibited an amphotropic h

45 MLVs with ecotropic host ranges show the greatest variability with

46 ased retroviral vectors with amphotropic and ecotropic host ranges.

47 The cell surface receptor for ecotropic host-range (infection limited to mice or rats)

48 says in T-cell lines, primary cells, and the ecotropic human immunodeficiency virus mouse model.

49 n their capacity to generate humantropic and ecotropic (i.e., pigtropic) virus.

50 tic transformation by competing with myeloid ecotropic integration site 1 for binding to the common h

51 MCAT-1.7 cells incubated with an ecotropic LacZ retrovirus, and this further increased wi

52 with the AdMad virus and then exposed to the ecotropic LacZ virus, gene transfer was virtually abolis

53 , known as Pbx and Meis (named for a myeloid ecotropic leukemia virus integration site), respectively

54 Murine ecotropic leukemia viruses use a common receptor for ent

55 er that also acts as the receptor for murine ecotropic leukemia viruses.

56 d viral infection composed of the inoculated ecotropic M-MuLV and polytropic MuLVs generated by recom

57 arallel increase in thymocytes that released ecotropic M-MuLV packaged within polytropic virions was

58 ts encoding the first 28 amino acids (aa) of ecotropic M-MuLV resulted in Env expression and binding

59 ously showed that B16 melanoma cells produce ecotropic melanoma-associated retrovirus (MelARV) which

60 The viral receptor for ecotropic MLV (eMLV), a classical model for retrovirus i

61 ilegus neonates were inoculated with Moloney ecotropic MLV (MoMLV).

62 se findings suggest a novel role for GRB2 in ecotropic MLV entry and infection by facilitating mCAT-1

63 s cell line is unique in its response to the ecotropic MLV envelope protein (Env) in that it undergoe

64 nonecotropic replacements in the progenitor ecotropic MLV genome are more extensive than previously

65 he spontaneous emergence of fully infectious ecotropic MLV in B6 mice with a range of distinct immune

66 In this assay, transduction by ecotropic MLV is a positive internal control for downstr

67 irulent (FrCasE) or nonneurovirulent (Fr57E) ecotropic MLVs influenced their viability and/or differe

68 ses (MLVs) are generated by recombination of ecotropic MLVs with members of a family of endogenous pr

69 d were even less closely related to those of ecotropic MLVs.

70 cells, which are normally resistant to mouse ecotropic MLVs.

71 are generated upon inoculation of different ecotropic MLVs.

72 of the subgenus Nannomys, are susceptible to ecotropic Moloney and Friend mouse leukemia viruses (MLV

73 id-to-lysine change at position 84 (D84K) of ecotropic Moloney MLV Env abolishes virus binding and in

74 0A was replaced by equivalent sequences from ecotropic Moloney MuLV (M-MuLV).

75 In the case of the ecotropic Moloney murine leukemia virus (M-MLV), the Nef

76 Here we analyze more than 40 derivatives of ecotropic Moloney murine leukemia virus (MLV) envelope,

77 elope (env) genes were generated between the ecotropic Moloney murine leukemia virus and the amphotro

78 els comparable to gene delivery by wild-type ecotropic Moloney murine leukemia virus and vesicular st

79 g a natural receptor-binding sequence of the ecotropic Moloney murine leukemia virus envelope glycopr

80 Here we report that tryptophan 142 in ecotropic Moloney murine leukemia virus envelope protein

81 f vectors coated with the parental wild-type ecotropic Moloney murine leukemia virus glycoprotein thr

82 imeras were previously generated between the ecotropic (Moloney-MuLV) and amphotropic (4070A) SU and

83 ORTANCE During virus-induced leukemogenesis, ecotropic mouse leukemia viruses (MLVs) recombine with n

84 y A-MuLV infection but not by infection with ecotropic MuLV (E-MuLV) (which utilizes a cationic amino

85 ve receptor-binding domain resembles that of ecotropic MuLV Env protein.

86 dditional exchange beyond the first 28 aa of ecotropic MuLV Env resulted in defective protein express

87 Although infection with ecotropic MuLV had no effect on activity of the mink CAT

88 Like other retroviruses, ecotropic MuLV infection eliminates virus-binding sites

89 However, ecotropic MuLV is considered to be a pH-dependent virus.

90 as increased 100- to 1,000-fold, whereas the ecotropic MuLV level remained unchanged.

91 as acquired the ability to interact with the ecotropic MuLV receptor on CHO-K1 cells that has undergo

92 SU and MuLV virions to cells expressing the ecotropic MuLV receptor, mCAT-1.

93 irus and an RNA transcript from an exogenous ecotropic MuLV RNA.

94 ine 4070A SU amino acids with the equivalent ecotropic MuLV sequence restored viral infectivity.

95 nd to viruses formed by recombination of the ecotropic MuLV with two distinct sets of polytropic env

96 O-K1 cells, which are generally resistant to ecotropic MuLV.

97 Resistance to ecotropic MuLVs appears to result from viral interferenc

98 mbinants the env gene sequences of exogenous ecotropic MuLVs are replaced with env gene sequences fro

99 (MuLVs) generated in mice after infection by ecotropic MuLVs can be classified into two major antigen

100 ping which occurred among the polytropic and ecotropic MuLVs in M-MuLV-infected mice.

101 Ecotropic MuLVs induce myeloid leukemia in BXH2 mice by

102 lytropic MuLVs generated by M-MuLV and other ecotropic MuLVs is the result of recombination with a si

103 NFS/N mice have been described and different ecotropic MuLVs preferentially recombine with different

104 In contrast to other ecotropic MuLVs such as Friend MuLV or Akv that recombin

105 fectious process of both the amphotropic and ecotropic MuLVs very likely involved endocytosis.

106 es (MuLVs) are generated by recombination of ecotropic MuLVs with env genes of a family of endogenous

107 All tumors tested expressed ecotropic MuLVs, and most expressed mink cell focus-indu

108 verlapped a heparin-binding domain common to ecotropic MuLVs.

109 me influence this difference between the two ecotropic MuLVs.

110 or other hamster cells resistant to ordinary ecotropic MuLVs.

111 city of recombination observed for different ecotropic MuLVs.

112 the envelope protein (gp70) of an endogenous ecotropic murine leukemia provirus.

113 Ecotropic murine leukemia retrovirus supernatant, collec

114 loped HIV-1 particles bearing both HIV-1 and ecotropic murine leukemia virus (E-MLV) Env proteins as

115 nd contained multiple clonal integrations of ecotropic murine leukemia virus (E-MuLV).

116 ar receptors for Rous sarcoma virus (Tva) or ecotropic murine leukemia virus (MCAT-1) were produced.

117 een reported to harbor an unusual infectious ecotropic murine leukemia virus (MLV) and proviral envel

118 The entry of ecotropic murine leukemia virus (MLV) into cells require

119 ring processes, we performed breeding of six ecotropic murine leukemia virus (MLV) strains by DNA shu

120 recently reported to be contaminated with an ecotropic murine leukemia virus (MLV), raising questions

121 y in NFS.N mice congenic for high-expressing ecotropic murine leukemia virus (MuLV) genes from AKR an

122 We show that fusion mediated by ecotropic murine leukemia virus envelope is dependent on

123 Ecotropic murine leukemia virus forms large syncytia wit

124 Entry of ecotropic murine leukemia virus initiates when the envel

125 , tyrosine 235 and glutamic acid 237, of the ecotropic murine leukemia virus receptor (ATRC1) have be

126 This 41-amino-acid region of Friend ecotropic murine leukemia virus SU was shown to be highl

127 In ecotropic murine leukemia virus SU, replacement of histi

128 The infectivity of Friend ecotropic murine leukemia virus was previously shown to

129 -1/NL4-3 was replaced with that of gp80 from ecotropic murine leukemia virus, a retrovirus that infec

130 Ag. gp70, the env product of the endogenous ecotropic murine leukemia virus, has been reported to be

131 vector RCASBP(A) with the env region from an ecotropic murine leukemia virus.

132 Susceptibility to ecotropic murine leukemia viruses (MLV) is restricted to

133 ive syncytium formation after infection with ecotropic murine leukemia viruses (MLVs) and is frequent

134 the Fv-4 gene are resistant to infection by ecotropic murine leukemia viruses (MuLVs).

135 BL6 melanoma cells produce melanoma-specific ecotropic murine leukemia viruses that encode serologica

136 In the receptor for ecotropic murine leukemia viruses, tyrosine 235 contribu

137 A small group of ecotropic murine retroviruses cause a spongiform neurode

138 hat the amino-terminal domain from the SU of ecotropic murine retroviruses contains an MCAT-1 binding

139 ly resistant to infection by amphotropic and ecotropic murine retroviruses, but they become susceptib

140 udotyped HIV-1 particles carrying either the ecotropic or the amphotropic Mo-MLV envelope proteins or

141 recombinants that lacked the type I-specific ecotropic p15E gene sequences.

142 ed using retroviral supematant from the BOSC ecotropic packaging cell line.

143 oviral supernatant obtained from the BOSC 23 ecotropic packaging cell line.

144 MLV host range subgroups in laboratory mice: ecotropic, polytropic, and xenotropic MLVs (E-, P-, and

145 Patterns of ecotropic proviral integration indicated that natural in

146 duction, 11 of 11 repopulated mice contained ecotropic provirus and 6 of 11 contained amphotropic pro

147 spicelegus was found to be equidistant from ecotropic provirus and nonecotropic provirus by phylogen

148 e defective Emv-2 provirus, a single copy of ecotropic provirus existing in the genome of C57BL/6 mic

149 vel proviral integration sites in a multiple ecotropic provirus mapping stock.

150 pic and amphotropic retrovirus transduction, ecotropic provirus sequences were detected in 10 of 13 l

151 ic PRR could functionally substitute for the ecotropic PRR.

152 2 leukemias encoding proto-oncogenes contain ecotropic rather than MRV viruses, it has been speculate

153 rine leukemia virus glycoprotein through the ecotropic receptor and approaching that of infection of

154 Cells coexpressing the ecotropic receptor and nucleus-localized beta-galactosid

155 The ecotropic receptor coding sequences in the rAAV genome w

156 , the green fluorescent protein (GFP)-tagged ecotropic receptor designated murine cationic amino acid

157 to transduce mouse cells bearing the natural ecotropic receptor was effectively lost.

158 Cells expressing the ecotropic receptor were efficiently transduced with an e

159 ly expressed Fv-4 env-encoded protein to the ecotropic receptor, although the immune system also play

160 To analyze the ecotropic receptor, CAT1, in XC cells, a mouse CAT1 tagg

161 ntly in avian DFJ8 cells that express murine ecotropic receptor.

162 l hydrophobic residue at position 235 of the ecotropic receptor.

163 d to induce syncytia in cells expressing the ecotropic receptor.

164 the rAAV genome and continued to express the ecotropic receptor.

165 melanoma was closely associated with C-type ecotropic retroviral particle production.

166 supernatant, a packaging cell line producing ecotropic retroviral particles carrying the HSV-TK (Herp

167 n to be susceptible to infection with murine ecotropic retroviral particles.

168 High-titer, ecotropic retroviral producer cells free of replication

169 HER-2/neu (Neu) by coculture with irradiated ecotropic retroviral producer lines.

170 ociated virus type 2 (AAV) vector containing ecotropic retroviral receptor (ecoR) cDNA under the cont

171 ein that has been shown to serve as a murine ecotropic retroviral receptor in transient assays, was c

172 hese cells constitutively express the murine ecotropic retroviral receptor MCAT-1 without changes in

173 mino acid transporter which functions as the ecotropic retroviral receptor, thereby rendering human c

174 more frequently in mice that lack endogenous ecotropic retroviral sequences and thus were not due to

175 receptor were efficiently transduced with an ecotropic retroviral vector encoding a nucleus-localized

176 nd the proportion of cells transduced by the ecotropic retroviral vector was decreased.

177 conditionally susceptible to infection by an ecotropic retroviral vector.

178 very of the function of the receptor for the ecotropic retrovirus as a membrane transporter for the e

179 transduced on days 2, 3, and 4 of culture by ecotropic retrovirus encoding human TGF-beta1 using cent

180 rom mutant mice were completely resistant to ecotropic retrovirus infection.

181 The polylysine modification and formation of ecotropic retrovirus molecular conjugates (eMMLV-PL) per

182 n proviral DNA and loss of melanoma-specific ecotropic retrovirus particle production.

183 at this MAA is encoded by the env gene of an ecotropic retrovirus produced by B16 melanoma cells.

184 amphotropic retrovirus receptor (amphoR) and ecotropic retrovirus receptor mRNA expression in highly

185 of amphoR mRNA and relatively high levels of ecotropic retrovirus receptor mRNA were studied.

186 populating hematopoietic stem cells using an ecotropic retrovirus resulted in an increase in T cells,

187 The wild mouse ecotropic retrovirus, Cas-Br-E, induces progressive, non

188 In principle, if ecotropic retrovirus, which is incapable of infecting hu

189 enable human cells to become permissive for ecotropic retrovirus-mediated gene transfer, we have dev

190 Similarly, ecotropic retrovirus-mediated Neo(r) transduction of pri

191 er of the y+ system and the receptor for the ecotropic retrovirus.

192 o be the sole receptor for a group of murine ecotropic retroviruses associated with hematological dis

193 this lysosomal storage disease, we produced ecotropic retroviruses encoding the human beta-hexosamin

194 surface epithelial cell line in culture with ecotropic retroviruses expressing a mutated rat neu/c-er

195 Upon infection of mice, ecotropic retroviruses recombine with endogenous proviru

196 lanoma cells contain at least four different ecotropic retroviruses with different insertion sites th

197 be functionally displayed on the surface of ecotropic retroviruses without interfering with their in

198 and KB, known to be nonpermissive for murine ecotropic retroviruses, became permissive for infection

199 ty locus (H-2) were injected with a modified ecotropic SL3-3 murine leukemia virus (MuLV) to determin

200 ing domain joined at the hinge region to the ecotropic SU C terminus.

201 ated that the hypervariable domain of Friend ecotropic SU does not contain any specific sequence or s

202 s which contained the N-terminal half of the ecotropic SU protein, encoding the receptor binding doma

203 n vitro studies indicated the virus to be an ecotropic subgroup A FeLV with 98% nucleotide sequence h

204 additions to the murine leukemia virus (MLV) ecotropic subgroup: Mus cervicolor isolate M813 and Mus

205 The ecotropic vector, RCASBP(Eco), was created by replacing

206 retrovirally transduced with amphotropic or ecotropic vectors encoding a normal acid sphingomyelinas

207 lts of these studies confirmed the fact that ecotropic vectors were much more efficient at transducin

208 ver genetically tropism-modified recombinant ecotropic vectors.

209 the effects of retroviral transduction using ecotropic versus amphotropic vectors, various growth fac

210 t a unifying mechanism whereby PDX1, myeloid ecotropic viral insertion site (MEIS), and pre-B-cell le

211 One common insertion site, the ecotropic viral insertion site 3 (Evi3), has been implic

212 , we showed that retroviral insertion at the ecotropic viral insertion site 32 locus leads to increas

213 so identified fusion transcripts between the ecotropic viral insertion site I (EVI1) gene in 3q26 and

214 rgets, including homeobox A9 (Hoxa9)/myeloid ecotropic viral integration 1 (Meis1)/pre-B-cell leukemi

215 e-B-cell leukemia homeobox (PBX) and myeloid ecotropic viral integration site (MEIS) proteins control

216 TED1-like homeobox (KNOX) and animal Myeloid ecotropic viral integration site (MEIS) proteins share a

217 Enhanced expression of ecotropic viral integration site 1 (EVI-1) occurs in app

218 Ecotropic viral integration site 1 (EVI1) is an oncogeni

219 etic cells the transforming potential of the ecotropic viral integration site 1 (Evi1) oncogene is th

220 Ecotropic viral integration site 1 (EVI1), a proto-oncog

221 Ecotropic viral integration site 1 (EVI1), required for

222 Myeloid ecotropic viral integration site 1 (Meis1) forms a heter

223 tential cooperative interaction with myeloid ecotropic viral integration site 1 homolog (MEIS1).

224 Meis1 (Myeloid Ecotropic viral Integration Site 1) is a homeobox gene t

225 netic and transcriptional signature of EVI1 (ecotropic viral integration site 1)-rearranged (EVI1-r)

226 1B transcriptional repressor, ETS variant 6, ecotropic viral integration site 1, and homeobox A11.

227 It is highly related to ecotropic viral integration site 3 (EVI3), a protein tha

228 Ecotropic viral integration site-1 (EVI1) and myelodyspl

229 Ecotropic viral integration site-1 (EVI1) is an oncogeni

230 rep1) is a tumor suppressor, whereas myeloid ecotropic viral integration site-1 (Meis1) is an oncogen

231 The proto-oncogene EVI1 (ecotropic viral integration site-1), located on chromoso

232 Blocking of the ecotropic viral receptor by secreted gp70 SU may contrib

233 greater and involves fusion-from-without by ecotropic virion particles.

234 otyping of the polytropic MuLV genome within ecotropic virions was nearly complete in coinoculated mi

235 ic MuLVs were extensively pseudotyped within ecotropic virions.

236 and release of the polytropic genomes within ecotropic virions.

237 LV genome was extensively pseudotyped within ecotropic virions; polytropic virus release was profound

238 All 17 inoculated mice produced infectious ecotropic virus after 8 to 14 weeks, and two unusual phe

239 behaves as expected for a process limited by ecotropic virus attachment to single receptors, fusion-f

240 The ecotropic virus component alone transferred some disease

241 to parental virus, the transgene-containing ecotropic virus demonstrated recombinogenic activity wit

242 Ecotropic virus did not disrupt Mrvi1 in 205 ecotropic v

243 ne, or a methionine at position 235 mediated ecotropic virus entry comparable to that mediated by ATR

244 nearby hydrophobic amino acid for efficient ecotropic virus entry.

245 Coinoculation of the ecotropic virus Friend MuLV (F-MuLV) with Fr98, a polytr

246 e latter route, the N-terminal domain of the ecotropic virus glycoprotein expressed on the cell surfa

247 dvanced age, over one-half were positive for ecotropic virus in tail or lymphoid tissues, and MCF vir

248 in amounts sufficient to block infection by ecotropic virus in vitro.

249 t from altered interactions with the unusual ecotropic virus mCAT1 receptor carried by M. dunni.

250 transinfection of PA317-tTA cells with TL-SN ecotropic virus particles.

251 ) of the homologous human protein that lacks ecotropic virus receptor capability resulted in acquisit

252 ceptor capability resulted in acquisition of ecotropic virus receptor function comparable to that of

253 ; and (iii) a loss of the use of the natural ecotropic virus receptor.

254 oundly elevated in coinfected cells, and the ecotropic virus release was unchanged.

255 ed MCF provirus and indicates that, like the ecotropic virus resistance gene Fv4, Rmcf may mediate re

256 We report that the infection of mice with an ecotropic virus results in pseudotyping of intact endoge

257 Ecotropic virus did not disrupt Mrvi1 in 205 ecotropic virus-positive leukemias, suggesting that Mrvi

258 Previous studies with ecotropic virus-specific polarity probes showed that the

259 characterized in more detail with additional ecotropic virus-specific probes that hybridized to the p

260 ain the 5' p15E (TM) gene sequences from the ecotropic virus.

261 ng to a different receptor than the parental ecotropic virus.

262 The receptor for ecotropic viruses is a transporter for basic amino acids

263 lish the interference-mediated resistance to ecotropic viruses produced by the Fv-4 gene.

264 All MoMLV-inoculated mice produced ecotropic viruses that resembled their MoMLV progenitor,

265 Ecotropic, xenotropic, and polytropic mouse leukemia vir

266 use different receptors for cell entry: the ecotropic, xenotropic, polytropic, amphotropic, 10A1, an