ライフサイエンスコーパス: ecto-ATPase

1 ockers of nitric oxide, GABAA, glutamate, or ecto-ATPase.

2 200 kDa band indeed represents the trimeric ecto-ATPase.

3 ecto-ATPase homooligomers would inhibit the ecto-ATPase.

4 adenosine A(1) receptors, ATP receptors, and ecto-ATPase.

5 ted by the corresponding region of the human ecto-ATPase.

6 a cells confirm that the new gene encodes an ecto-ATPase.

7 bile acid transport mechanism distinct from ecto-ATPase.

8 le library to identify the cDNA encoding the ecto-ATPase.

9 new opportunity to identify the canalicular ecto-ATPase.

10 o identify non-cross-linked and cross-linked ecto-ATPase.

11 more potent inhibitor of ecto-apyrases than ecto-ATPases.

12 eins, is observed only in the membrane-bound ecto-ATPases.

13 In the sequence of the CR1 of human ecto-ATPase, 58WPADKENDTGIV69, 65DTG67 is similar to the

14 In addition, cellular ecto-ATPase activities were measured following prolonged

15 cells transfected with CD39 possess similar ecto-ATPase activities, further supporting the conclusio

16 in aggregation is distinct from its role in ecto-ATPase activity and the aggregating properties cann

17 s was developed to explain the modulation of ecto-ATPase activity by a variety of agents, including d

18 restingly, under nonreducing conditions, the ecto-ATPase activity in rat and pig (unlike chicken and

19 Human ecto-ATPase activity is decreased by NP-40 and at temper

20 consensus sequence completely eliminates the ecto-ATPase activity of CBATP.

21 onditions, was observed only when endogenous ecto-ATPase activity was pharmacologically inhibited by

22 ry staining, we also demonstrated functional ecto-ATPase activity within the odontoblast layer, subod

23 In addition to the existence of an ecto-ATPase activity, our results suggest a major scaven

24 amino acid protein (NTPDase2 alpha exhibited ecto-ATPase activity.

25 contain both constitutive and TCDD-inducible ecto-ATPase activity.

26 s actual release as the cells also displayed ecto-ATPase activity.

27 Transgenic upregulation of ecto-ATPase alone confers resistance to organisms that h

28 of either the C- or N-terminus of the human ecto-ATPase alone in the chicken ecto-ATP-diphosphohydro

29 they are promptly degraded into adenosine by ecto-ATPase and 5'-nucleotidase, which have been identif

30 absence of ATP was inhibited by apyrase, an ecto-ATPase and by suramin, an antagonist of P2 receptor

31 The sequence homology between the gizzard ecto-ATPase and CD39 was confirmed by Western blots demo

32 endothelial cell membrane protein with both ecto-ATPase and ecto-ADPase activities.

33 ed the in vivo localization of extracellular ecto-ATPase and ecto-apyrase (ATPDase) in adult chicken

34 Purification and molecular cloning of ecto-ATPase and other canalicular proteins are complicat

35 In contrast to Mrp2, protein expression of ecto-ATPase and P-gp remained unchanged in endotoxin- an

36 clonal antibodies raised against the chicken ecto-ATPase and two commercially available monoclonal an

37 ary structures, the results suggest that the ecto-ATPases and ecto-apyrases are part of, or closely r

38 ST programs demonstrated homology with other ecto-ATPases and ecto-apyrases, including those from the

39 ases have been divided into two subfamilies, ecto-ATPases and ecto-ATP-diphosphohydrolases (ecto-ATPD

40 yrase conserved regions, are present in both ecto-ATPases and soluble E-type ATPases.

41 their ability to cross-react with mammalian ecto-ATPases and were used as specific immunochemical pr

42 f canalicular ecto-adenosine triphosphatase (ecto-ATPase) and mdr P-glycoproteins (P-gp).

43 in that is thought to have bile acid efflux, ecto-ATPase, and cell adhesion properties.

44 ctional protein possessing bile acid efflux, ecto-ATPase, and intercellular aggregating properties.

45 millimolar concentrations of azide, whereas ecto-ATPases appear to lack these two properties.

46 However, the recent cloning of an ecto-ATPase (apyrase) from potato tubers provides a new

47 However, the structural features of this ecto-ATPase are not those anticipated for an in-to-out A

48 degradation: Pannexin1, TRPM5, and NTPDase2 (ecto-ATPase) are indistinguishable between WT and DKO mi

49 ber (Solanum tuberosum) apyrase and parasite ecto-ATPase, are not affected by detergents.

50 that ATP release sites are colocalized with ecto-ATPases at the astrocyte cell surface.

51 y is abolished by prior cross-linking of the ecto-ATPase by lectin and chemical cross-linking agents.

52 ATP is rapidly hydrolyzed by the ecto-ATPase CD39 into adenosine monophosphate (AMP), and

53 sine generation from pericellular ATP is the ecto-ATPase CD39, we next show that inhibition of CD39 a

54 Ecto-ATPase (CD39L1) corresponds to the type 2 enzyme of

55 A full-length E(ecto)-ATPase cDNA was cloned from a human brain cDNA lib

56 rat liver canalicular bile acid transporter/ecto-ATPase/cell CAM 105 (CBATP) is a 110-kDa transmembr

57 rat liver canalicular bile acid transporter/ecto-ATPase/cell CAM 105 (CBATP), a member of the carcin

58 ecent studies have established that dominant ecto-ATPases consist of enzymes now called nucleoside tr

59 , sensitivity to alphabeta-methylene ATP and ecto-ATPase-dependent recovery from endogenous desensiti

60 TM cells expressed ecto-ATPases E-NPP1-3, E-NTPD2, E-NTPD8, and CD73.

61 Human ecto-ATPase (E-NTPDase 2) and chicken ecto-ATP-diphospho

62 converted the eNTPDase-3 ecto-apyrase to an ecto-ATPase (eNTPDase-2), mainly by decreasing the hydro

63 ount for the observed species differences in ecto-ATPase enzymatic properties.

64 lts obtained with the rabbit skeletal muscle ecto-ATPase enzyme, cross-linking the chicken gizzard sm

65 he presence of one or more calcium-dependent ecto-ATPases (enzymes that hydrolyze extracellular 5'-tr

66 Bile acid transport activity and ecto-ATPase expression were analyzed in primary rat hepa

67 This is the second ecto-ATPase family member and the first ecto-ATPDase to

68 iphosphohydrolase (ATPDase), a member of the ecto-ATPase family, was purified to homogeneity previous

69 sed the existence and functional activity of ecto-ATPase for extracellular ATP degradation.

70 se could effectively compete with endogenous ecto-ATPases for released ATP.

71 The ecto-ATPase from chicken gizzard (smooth muscle) was sol

72 Ecto-ATPases from transformed human trabecular meshwork

73 Induction of ecto-ATPase gene expression by TCDD is direct and occurs

74 30 kDa immunoreactive band, proposed to be a ecto-ATPase homodimer, and a concomitant decrease in a a

75 whereas agents and conditions destabilizing ecto-ATPase homooligomers would inhibit the ecto-ATPase.

76 d to be an intermolecularly disulfide-linked ecto-ATPase homotrimer.

77 d RNA analyses showed very low expression of ecto-ATPase in HTC and HTC-R cells compared with hepatoc

78 The presence of ecto-ATPase in taste buds likely reflects the importance

79 e canalicular ecto-adenosine triphosphatase (ecto-ATPase) in adenosine triphosphate (ATP)-dependent b

80 was readily measured even in the absence of ecto-ATPase inhibition suggesting that the spatially col

81 opylxanthine) (10 nm) and was reduced by the ecto-ATPase inhibitor ARL-67156 (6-N,N-diethyl-D-beta,ga

82 use intravesical perfusion of apyrase or the ecto-ATPase inhibitor ARL67156 altered reflex bladder ac

83 entiated by alphabetam-ATP as well as by the ecto-ATPase inhibitor ARL67156 and were depressed in the

84 e receptor antagonist, MRS1754, ARL67156, an ecto-ATPase inhibitor, and A2A receptor siRNA, suggestin

85 wild-type mice of either exogenous ATP or an ecto-ATPase inhibitor, ARL-67156, and by exposure of hep

86 The chicken muscle ecto-ATPase is a slightly basic (predicted pI = 7.93) 49

87 ted by the corresponding region of the human ecto-ATPase is also inhibited by NP-40 and is less activ

88 cto-ATPDase with other reported ATPDases and ecto-ATPases is discussed.

89 The extracellular ATPase (ecto-ATPase) is a divalent cation-dependent nucleoside t

90 The results suggest that the muscle ecto-ATPase may be involved in cell adhesion, since the

91 es that recognize the 66 kDa chicken gizzard ecto-ATPase monomer strengthened the hypothesis that thi

92 pproximately 66 kDa immunoreactive band, the ecto-ATPase monomer.

93 After reduction, ecto-ATPase monomers were found to be approximately 66 k

94 The human ecto-ATPase (NTPDase 2) contains conserved motifs includ

95 s correspond to splice variants of the human ecto-ATPase (NTPDase2 alpha,-2 beta, and -2 gamma).

96 posed that agents and conditions stabilizing ecto-ATPase oligomers stimulate enzyme activity, whereas

97 The presence of ecto-ATPases on ARPE-19 cell membranes was suggested by

98 Cell surface ATPases (ecto-ATPases or E-ATPases) hydrolyze extracellular ATP a

99 D1, and ecto-5'-nucleotidase, but not NTPD2 (ecto-ATPase, or CD39L1), in the rabbit NPE cells.

100 as solubilized, and the 66-kDa cell membrane ecto-ATPase protein was purified.

101 pecific ways to stabilize the native, active ecto-ATPase quaternary structure (the homotrimer).

102 The differences in ecto-ATPase quaternary structure stability may account f

103 The chicken ecto-ATPase showed considerable amino acid sequence homo

104 d sequence suggests that the gene encodes an ecto-ATPase that contains multiple glycosylation sites,

105 ytes, of one or more proteins other than the ecto-ATPase that mediate ATP-dependent transport of bile

106 in determining the sensitivity of the human ecto-ATPase to NP-40 and high temperatures; (2) incorpor

107 However, ecto-ATPase was clustered in the sarcolemma of the organ

108 In chicken gizzard, the ecto-ATPase was distributed in discrete clusters restric

109 Ecto-ATPase was immunochemically identified in chicken,

110 the activity and cellular trafficking of the ecto-ATPase were examined.

111 ies, both raised against the chicken gizzard ecto-ATPase, were evaluated for their ability to cross-r

112 re, and substrate resemble that of the human ecto-ATPase, which donates the C-terminus including the

113 ecto-ATPDase is equally distant from the two ecto-ATPases, which exhibit low activity toward ADP, and

114 ss-linking the chicken gizzard smooth muscle ecto-ATPase with 3,3'-dithiobis(sulfosuccinimidylpropion