ライフサイエンスコーパス: edit

1 h are collectively referred to as 'epigenome editing'.

2 ay depending on the occurrence and extent of editing.

3 ing the target range of Cpf1-mediated genome editing.

4 ecificity factors of cytidine to uridine RNA editing.

5 cted repair regulates the SNGD-mediated gene editing.

6 PR-associated) homology-directed repair gene-editing.

7 t Cas9-mediated allotetraploid cotton genome editing.

8 ce of clinical applications involving genome editing.

9 c gene insertion by homology-directed genome editing.

10 es efficient nucleotide substitution by gene editing.

11 affect cis-regulatory elements to alter RNA editing.

12 isogenic iPSC lines using CRISPR/Cas9 genome editing.

13 indromic repeats (CRISPR-Cas9)-mediated gene editing.

14 , were designed for site specific epigenetic editing.

15 nding of the biological importance of A-to-I editing.

16 of synthetic donor DNAs for efficient genome editing.

17 n of target genes undergoing extensive 3'UTR editing.

18 cell stage, but become Env(-) upon receptor editing.

19 number of sites that can be targeted by base editing 2.5-fold.

20 The following are edited/abbreviated versions of the annual reports of the

21 erent ADAR1 binding behaviors related to its editing activity, as well as the antagonizing effect of

22 than -6.0 Kcal/mole and for all of them the edited adenosines mis-paired with cytosines on the pre-m

23 nocarriers delivering mRNA encoding a genome-editing agent can efficiently knock-out selected genes i

24 Expression of the wild-type or edited allele of AMD1 but not un-editable allele rescued

25 and structural requirements of pre-miRNA for editing along with a suggestive crucial role for ADAR2.

26 ly edited transcripts within it to show that editing alters gene expression by modulating translation

27 scripts that are targets of editing and that editing alters their function.

28 tudy reveals widespread cis variation in RNA editing among genetically distinct individuals and sheds

29 We demonstrate the use of CRISPR-Cas9 to edit an endogenous insect cell gene and alter protein gl

30 scovered, the extent to which most sites are edited and how the editing is regulated in different bio

31 ndrial mRNAs undergo internal changes by RNA editing and 3' end modifications.

32 inhibition using CRISPR/Cas-mediated genome editing and demonstrate that KRAS is dispensable in a su

33 ammable meganucleases is transforming genome editing and functional genomics.

34 r and have important implications for genome editing and genome evolution.

35 ry, SNGD promotes precise and efficient gene editing and may be a promising strategy for the developm

36 based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-derivatives in se

37 enriched for transcripts that are targets of editing and that editing alters their function.

38 ags as fusion partners in Cas9-mediated gene editing and the construction of doubly DNA-tethered prot

39 as been widely adopted for RNA-guided genome editing and transcription regulation in applications suc

40 Finally, genome-editing and transgenic approaches demonstrate that a hig

41 Here we implement CRISPR-Cas9 genome editing and transposon-mediated somatic gene transfer to

42 terface with which Python programs can read, edit, and write NMR-STAR formatted files and their equiv

43 cope of applications in nanotechnology, gene editing, and DNA library construction.

44 Advances in chemical genomics, gene editing, and model systems now permit deconstruction of

45 Adaptation of CRISPR-Cas9 for genome-editing applications has revolutionized biomedical resea

46 he time and cost of in vitro or ex vivo gene-editing applications in precision medicine and drug disc

47 ian cells and are thus precluded from genome-editing applications.

48 powerful tools for gene transfer and genome editing applications.

49 This genome editing approach has the advantage that it does not requ

50 Here we report a genome editing approach in which adeno-associated virus (AAV)-m

51 reporter assays, we sought to develop a gene editing approach to investigate the regulatory activity

52 We developed a genome-editing approach to target a dominantly inherited form o

53 , CRISPR-Cas9 tools for site-specific genome editing are needed to facilitate further improvements in

54 ese mutant collections, together with genome editing, are being used in polyploid species to combine

55 player in memory and establishes epigenetic editing as a potential therapy to treat human neurologic

56 numerous sites of insertion versus deletion editing as editosomes collaborate to accurately edit tho

57 The rate of miR487b editing, as well as 2'-O-ribose-methylation, is increase

58 38 mitochondrial editing sites and increased editing at 24 sites; therefore the absence of MEF8 affec

59 -DNA insertion (mef8) line exhibited reduced editing at 38 mitochondrial editing sites and increased

60 while still promoting integration of genome edits at rates of approximately 50% without selection in

61 Here the authors show, using CRISPR gene editing, ATAC-seq and ChIP-seq, that specific Runx1-boun

62 n for its promise in basic research and gene editing-based therapeutics.

63 res, and the set of genes differentially RNA-edited between case and control mice were enriched for f

64 ding inefficiencies in targeted nuclear gene editing broadly hinder Chlamydomonas research.

65 lles, with predominant specificity for sites edited by E(+)-type PPR proteins.

66 for efficient sporulation and suggests that editing by aminoacyl-tRNA synthetases may be important f

67 key features of current knowledge of genomic editing by CRISPR/Cas9 technology as a feasible strategy

68 First, we greatly reduce off-target base editing by installing mutations into our third-generatio

69 vides insights into the mechanism of peptide editing by TAPBPR and, by analogy, tapasin.

70 ble of robust knockdown and demonstrated RNA editing by using catalytically inactive Cas13 (dCas13) t

71 Finally, we show how inducible gene editing can be achieved by combining the TAEL and CRISPR

72 e transformants demonstrate that plastid RNA editing can be bypassed through the expression of nucleu

73 CRISPR/Cas9-based genome editing can easily generate knockout mouse models by dis

74 Apolipoprotein B mRNA-editing catalytic polypeptide (APOBEC) 3 proteins have b

75 r, we demonstrate that apolipoprotein B mRNA-editing catalytic polypeptide 3 expression and editing f

76 , we demonstrated that apolipoprotein B mRNA-editing catalytic polypeptide 3A (A3A) and A3G expressio

77 Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by Adenosine DeAminases acting on dou

78 Ablation of PheRS editing caused accumulation of Tyr-tRNAPhe (5%), but not

79 ells, but also enhances T-cell potency, with edited cells vastly outperforming conventionally generat

80 a modified XCAT phantom with additional and edited cerebrospinal fluid (CSF) regions to produce voxe

81 ion, protein replacement therapy, and genome editing, collectively affecting approaches for the preve

82 biquitin protein ligase (ITCH)-A20 ubiquitin-editing complex inhibits receptor-interacting Ser/Thr ki

83 ly used in other lab animals to deliver gene editing constructs have been less effective in songbirds

84 metabolic disease modeling by somatic genome editing could be applied to many other systemic as well

85 ggest that TCR transfer combined with genome editing could lead to new, improved generations of cance

86 An editing defect, leading to an amino acid change, in the

87 and DYW2-GFP overexpressing lines show broad editing defects in both organelles, with predominant spe

88 fficiency could however not be linked to any editing defects in the nad2 transcript.

89 Two cell colonies were successfully gene edited demonstrating the desired mutation.

90 The editing-dependent stabilization of mRNAs in turn alters

91 In light of recent human embryo editing developments, scientists and stakeholders from a

92 Hereby, we use normalized edit distance to enhance dimensionality reduction method

93 By combining normalized edit distance with Isomap and Laplacian Eigenmaps, synth

94 CRISPR/Cas9 is a promising tool for genome-editing DNA in cells with single-base-pair precision, wh

95 ually synthesized with associated acyl chain editing during nitrogen stress, in contrast to an overal

96 g mutations and context-dependence of genome editing efficiency that would confound other strategies.

97 We showed that CRIPSR-mediated genome editing efficiently excised the mutant exon 23 in dystro

98 62 in response to shRNA knockdown of the RNA editing enzyme ADAR1.

99 antibody gene deaminase AID and the RNA/DNA editing enzyme APOBEC1 (A1).

100 The apolipoprotein B mRNA editing enzyme catalytic polypeptide-like APOBEC3A and A

101 Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) prot

102 These phenotypes were mimicked by SHANK3-edited ES cells and rescued by transduction with a Shank

103 The majority of editing events alter the sequence of the 3'UTR of target

104 Tens of thousands of A-to-I editing events are defined in the mouse, yet the functio

105 transcriptomes, cancer-specific recoding RNA editing events have yet to be discovered.

106 e polymorphisms (SNPs) in the genome, or RNA editing events within the RNA.

107 have reported key roles for individual miRNA editing events, but a comprehensive picture of miRNA edi

108 logy; functional and comparative OMICs; gene editing; expanded use of model organisms; and a new sing

109 Interestingly, gene silencing or editing experiments revealed that SNAT7 is the primary p

110 matR transcripts' sites showed a decrease of editing extent in the mef8 mutant.

111 tidial CHLOROPLAST BIOGENESIS 19 (CLB19) PPR editing factor.

112 e protein interactions between the different editing factors are still poorly understood.

113 d through the expression of nucleus-encoded, edited forms of plastid genes.

114 MSTN-edited fry had more muscle cells (p < 0.001) than contro

115 n controls, and the mean body weight of gene-edited fry increased by 29.7%.

116 iting catalytic polypeptide 3 expression and editing function was heat sensitive to a certain degree,

117 isacylation through a separate post-transfer editing function.

118 CRISPR/Cas9 genome editing generated predicted null mutations in cnrip1a an

119 ecular mechanism and different strategies to edit genes using the CRISPR-Cas9 system.

120 The revolution in CRISPR-mediated genome editing has enabled the mutation and insertion of virtua

121 The introduction of Cas9-directed genome editing has expanded adoption of this approach.

122 Genome editing has potential for the targeted correction of ger

123 trate that AAV-mediated muscle-specific gene editing has significant potential for therapy of neuromu

124 Focusing on the RNA editing hotspot in miR-200b, a key tumor metastasis supp

125 /CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness u

126 g of psbF-C77 and the reduction in accD-C794 editing in Arabidopsis (Arabidopsis thaliana).

127 The degree of differential RNA editing in epileptic mice correlated with frequency of s

128 Our study highlights the importance of miRNA editing in gene regulation and suggests its potential as

129 events, but a comprehensive picture of miRNA editing in human cancers remains largely unexplored.

130 We utilized CRISPR/Cas9 genome editing in human induced pluripotent stem (iPS) cell-der

131 Like bone marrow-derived macrophages, RNA editing in MG leads to overall changes in the abundance

132 Editing in microRNAs, particularly in seed can significa

133 educe hypoxia-induced gene expression or RNA editing in monocytes.

134 To establish CRISPR-directed gene editing in N. vitripennis, we targeted a conserved eye p

135 Gang et al. report CRISPR/Cas9 genome editing in parasites of the genus Strongyloides, generat

136 ker and allows sophisticated markerless gene editing in situ.

137 sibility of CRISPR/Cas9-based cardiac genome editing in vivo in postnatal mice.

138 and serial dosing regimens for somatic gene editing in vivo.

139 live mice to achieve specific, DNA-free base editing in vivo.

140 r techniques can produce high frequency gene editing in X. laevis, permitting analysis in the F0 gene

141 SLA class I is a target for genome editing in xenotransplantation.

142 the authors show high efficient single-base editing in zebrafish using modified Cas9 and its VQR var

143 desired clones with CRISPR-mediated genomic edits in a large number of samples is made possible by n

144 ollen-specific phospholipase, and that novel edits in MTL lead to a 6.7% haploid induction rate (the

145 ated stabilization of Ctn RNA occurred in an editing-independent manner.

146 Base editing induces single-nucleotide changes in the DNA of

147 luding gene overexpression, CRISPR/Cas9 gene editing, inducible technologies, optogenetic or DREADD c

148 ADAR3) as an important regulator of Q/R site editing, investigate its mode of action, and detect elev

149 Adenosine-to-inosine (A-to-I) RNA editing is a conserved post-transcriptional mechanism me

150 We conclude that CRISPR-Cas9-mediated genome editing is a powerful method for investigating gene func

151 Targeted epigenome editing is an emerging technology to specifically regula

152 RNA editing is an essential post-transcriptional process tha

153 Editing is enriched in the nervous system, affecting mol

154 The application of base editing is limited by off-target activity and reliance o

155 However, R/G editing is only effective in flop channels.

156 We here show that RNA editing is particularly common in behaviorally sophistic

157 Large-scale studies have revealed how editing is regulated both in cis and in trans.

158 t to which most sites are edited and how the editing is regulated in different biological contexts ar

159 t for few mammalian conserved editing sites, editing is significantly higher in neurons than in other

160 While the primary role of aaRS editing is to prevent misaminoacylation, we demonstrate

161 monstrate that the lifetime absence of ADAR1-editing is well tolerated in the absence of MDA5.

162 SNGD-mediated gene editing led to a markedly lower indel frequency than tha

163 tasis suppressor, we found that the miR-200b editing level correlates with patient prognosis opposite

164 o demonstrate that while temperature affects editing levels at more sites than genetic differences, g

165 Further, NUP43 exhibits constant editing levels between single cells, while GRIA2 editing

166 We show that editing levels in non-repetitive coding regions vary mor

167 oding regions vary more between tissues than editing levels in repetitive regions.

168 ization of mRNAs in turn alters the observed editing levels in the stable RNA repertoire.

169 ing levels between single cells, while GRIA2 editing levels vary.

170 of which are diversified by splicing and RNA editing, localize to >20 excitatory and inhibitory neoco

171 marcated by the gRNAs in the vicinity of the edited locus.

172 iting damage and simplifying the delivery of editing machinery.

173 ed three-dimensional gamma-aminobutyric acid-edited magnetic resonance (MR) spectroscopic imaging in

174 found that GeoCas9 is an effective tool for editing mammalian genomes when delivered as a ribonucleo

175 Although RNA editing markedly increases complexity of the cancer cell

176 More than half of the edited miRNAs showed increased stability, 72.7% of which

177 onal alleles and normal phenotypes in CRISPR-edited mutants.

178 the temperature-dependent function of a gene-edited mutation and provides a new method for the more g

179 elf-Thy-1 ligand, immunoglobulin light chain editing occurred, generating B cells with up-regulated N

180 Using CRISPR-Cas9 genome editing of bptf in zebrafish to induce a loss of gene fu

181 ell as for adenosine to inosine (A to I) RNA editing of Ctn RNA in muscle cells.

182 acterization as well as detailed interactive editing of filopodia reconstructions through an intuitiv

183 ansduces ECs of pathologic vessels, and that editing of genomic VEGFR2 locus using rAAV1-mediated CRI

184 Genome editing of human induced pluripotent stem cells (hiPSCs)

185 to prevent misaminoacylation, we demonstrate editing of misaminoacylated tRNA is also required for de

186 intravenous injection into mice induces >80% editing of Pcsk9 in the liver.

187 Precise genome editing of plants has the potential to reshape global ag

188 We demonstrate editing of post-mitotic neurons in the adult mouse brain

189 Here, we demonstrate that CRISPR/Cas9 genome editing of promoters generates diverse cis-regulatory al

190 N6 (ORRM6) result in the near absence of RNA editing of psbF-C77 and the reduction in accD-C794 editi

191 We used CRISPR/Cas9-mediated gene editing of the Ern1 locus to study the role of the TMD i

192 le-restricted Cas9 expression enables direct editing of the mutation, multi-exon deletion or complete

193 man salivary ductal cells through epigenetic editing of the native promoter.

194 e of gene activation/expression and receptor editing of these isotypes have not been studied.

195 d through pharmacologic inhibition or genome editing of these loci.

196 CRISPR/Cas9-based genome editing offers the possibility to knock out almost any g

197 t PSC-derivatives in several tissues of gene-edited organogenesis-disabled mice.

198 g their deaminase recruitment strategies and editing outcomes, and compare them to other CRISPR genom

199 docannabinoids signaling pathway and the RNA editing pathway were found to be dysregulated in EC.

200 sponsible for alterations in the tissue-wide editing patterns upon injury.

201 time-consuming and costly, generating genome-edited pigs holds great promise for agricultural, biomed

202 REPAIR presents a promising RNA-editing platform with broad applicability for research,

203 Here, we review the different base-editing platforms, including their deaminase recruitment

204 Our findings suggest miR487b editing plays an intricate role in postischemic neovascu

205 are confirmed to harbor an appropriate gene edit, pluripotency, differentiation potential, and genom

206 The gene editing potential of CRISPR/Cas9 encapsulated by ZIF-8 (

207 leads to overall changes in the abundance of edited proteins that coordinate the function of multiple

208 As genome editing rapidly progresses toward the realization of its

209 an genome is challenging with non-viral gene-editing reagents, since most of the edited sequences con

210 Base editing relies on recruitment of cytidine deaminases to

211 n zygotes, its application in postnatal gene editing remains incompletely characterized.

212 Overall, single TCR gene editing represents a clinically feasible approach that i

213 Loss of CXCR7 expression by CRISPR-Cas9 gene editing resulted in a halt of cell proliferation, severe

214 orter gene assays, we could demonstrate that editing results in a complete switch of target site sele

215 In vivo genome editing results in lower IOP and prevents further glauco

216 Epigenomic profiling and genome editing revealed that AMIGO2 is regulated by a melanoma-

217 CRISPR-Cas9 gene editing revealed that both BTK and B lymphocyte kinase (

218 ines have been repurposed to enable a genome editing revolution.

219 (for example, mutagenesis, CRISPR-based gene editing, RNA interference, morpholinos or pharmacologica

220 ral gene-editing reagents, since most of the edited sequences contain various imprecise insertions or

221 xhibited reduced editing at 38 mitochondrial editing sites and increased editing at 24 sites; therefo

222 Although many editing sites have recently been discovered, the extent

223 However, the vast majority of these RNA editing sites have unknown functions and are in noncodin

224 ate to accurately edit thousands of distinct editing sites in vivo.

225 The genomic sequence flanking editing sites is highly conserved, suggesting that the p

226 und that, except for few mammalian conserved editing sites, editing is significantly higher in neuron

227 A related approach uses AAV vectors to edit specific regions of the DMD gene using CRISPR/Cas9.

228 le further optimization of Cas9-based genome-editing specificity and efficiency.

229 nd are regulated by post-transcriptional RNA editing, splice variation, post-translational modificati

230 andidate functional variants and used genome-edited stem cells, CRISPR interference, and mouse modeli

231 as two antagonistic effects on mitochondrial editing: stimulatory, which requires a catalytic glutama

232 transgenic cells can be used for other gene-editing studies and is well-suited for high-throughput s

233 The CRISPR-Cas9 genome-editing system is a part of the adaptive immune system i

234 efficient delivery of the CRISPR-Cas9 genome-editing system to target cells in human body.

235 Here, we report a programmable "base editing" system to induce precise base conversion with h

236 evasion of one but not both tRNA synthetase editing systems.

237 shown to be an efficient and accurate genome-editing technique.

238 Then, we discuss how genome editing techniques enable a radically new set of approac

239 , induced mutations, and the advanced genome-editing technologies can be applied to improving the nut

240 the powerful combination of iPSCs and genome editing technologies for understanding the biological fu

241 Gene-editing technologies have made it feasible to create non

242 Recent advancement in genome editing technologies offers a promising therapeutic appr

243 mes, and compare them to other CRISPR genome-editing technologies.

244 ce of human biomaterials and the use of gene-editing technologies.

245 Using gene editing technology (CRISPR/Cas9), the SIRT1 gene was rem

246 We developed the TCR gene editing technology that is based on the knockout of the

247 b2 in neural development, we utilized genome-editing technology to generate an allelic series in the

248 Here the authors employ CRISPR/Cas9 gene editing technology to silence VEGFR2, a major regulator

249 CRISPR-Cas9 is a genome editing technology with major impact in life sciences.

250 CRISPR/Cas is a revolutionary gene editing technology with wide-ranging utility.

251 e of mutations generated by CRISPR/Cas9 gene-editing technology, and alleles designed to be null can

252 CRISPR-Cas9 has become a facile genome editing technology, yet the structural and mechanistic f

253 s/CRISPR-associated gene9 (CRISPR/Cas9) gene editing technology.

254 it is inappropriate to perform germline gene editing that culminates in human pregnancy.

255 We developed a strategy for precise gene editing that does not generate DSBs.

256 equencing, genetic complementation, and gene editing, that haploid induction in maize (Zea mays) is t

257 quence truncation procedure is then used for editing the models based on local variations of the stru

258 ADAR2 interacts with and edits the 3UTR of nuclear-retained Cat2 transcribed nucl

259 ting as editosomes collaborate to accurately edit thousands of distinct editing sites in vivo.

260 nated regulation of organellar multiple site editing through DYW2, which probably provides the deamin

261 lating centrosome activities, we used genome editing to ablate it.

262 derscore the potential of CRISPR/Cas9 genome editing to advance immunotherapies.

263 at were engineered by CRISPR-mediated genome editing to controllably release GLP-1 (glucagon-like pep

264 Here we used CrispR-Cas9-mediated gene editing to delete the gene encoding for AC, ASAH1, in hu

265 Here we applied CRISPR-Cas9 genome editing to disrupt the endogenous human MRP RNA locus, t

266 Here we use CRISPR-Cas9-mediated genome editing to investigate the function of the pluripotency

267 In this study, we used genome editing to knockout the two mcoln1 genes present in Dani

268 Towards this end, we used CRISPR-Cas9 genome editing to make a single allele knock-in of the most com

269 -electrochemistry and site-selective isotope editing to monitor the CO/CN(-) stretching vibrations in

270 ompelling demonstration of the power of gene editing to rapidly improve yield traits in crop breeding

271 , we apply the approach of site-directed RNA editing to repair, at the mRNA level, a disease-causing

272 Here we used CRISPR/Cas9 genome editing to separate catalytic activity-dependent and ind

273 With CRISPR/Cas9-mediated gene editing to stably knock out and recover Rab8a in macroph

274 Here we apply CRISPR-Cas9 gene editing to tag a cytoskeletal protein (alpha-tubulin) an

275 he CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to recruitment of

276 harnessed as a powerful and versatile genome-editing tool and holds immense promise for future therap

277 CRISPR is a versatile gene editing tool which has revolutionized genetic research i

278 s question, we use a CRISPR-dCas9 epigenetic editing tool, where an inactive form of Cas9 is fused to

279 nd plasmids, as well as a revolutionary gene editing tool.

280 tors provide an organized comprehensive gene editing toolbox of considerable scientific value.

281 the capabilities of the RNP-mediated genome editing toolbox.

282 Here, we employ diverse CRISPR/Cas9 genome editing tools to generate a series of targeted lesions w

283 teocytic cell lines-together with new genome editing tools-has allowed a closer look at the biology a

284 visualize and quantify adenosine-to-inosine-edited transcripts in situ.

285 ype (monocytes) and on a small set of highly edited transcripts within it to show that editing alters

286 repair (UBER) enzymes target and cleave A3G-edited uridine-containing viral cDNA.

287 of postnatal CRISPR/Cas9-based cardiac gene editing using adeno-associated virus serotype 9 to deliv

288 ic-transfection technique for precise genome editing using CRISPR-Cas9.

289 on was corrected by CRISPR/Cas9-based genome editing (V247fs-MT-correction).

290 Nicotiana benthamiana (16c) plants, and gene editing was accompanied by loss of GFP expression.

291 CRISPR/Cas9 gene editing was used to knock out pig conceptus IL1B2 expres

292 vage is a likely point of regulation for RNA editing, we elucidated endonuclease specificity in vivo.

293 HIV provirus gene-editing were confirmed by cell genomic DNA PCR and fluor

294 and "seizures." Genes with differential RNA editing were preferentially enriched for genes with a ge

295 rominently reduces the workload of cell-line editing, which may be completed within 4 weeks.

296 aminase domains that narrow the width of the editing window from approximately 5 nucleotides to as li

297 ZNP delivery of sgRNA enables permanent DNA editing with an indefinitely sustained 95 % decrease in

298 Here we combine CRISPR/Cas9 gene editing with an innovative high-throughput genotyping pi

299 iew this RNA-guided nuclease system for gene editing with respect to its usefulness for cardiovascula

300 cola, allowed efficient Cas9-mediated genome editing without the need for a repair template.