ライフサイエンスコーパス: editing

1 h are collectively referred to as 'epigenome editing'.

2 ing the target range of Cpf1-mediated genome editing.

3 ecificity factors of cytidine to uridine RNA editing.

4 PR-associated) homology-directed repair gene-editing.

5 cted repair regulates the SNGD-mediated gene editing.

6 t Cas9-mediated allotetraploid cotton genome editing.

7 ce of clinical applications involving genome editing.

8 c gene insertion by homology-directed genome editing.

9 es efficient nucleotide substitution by gene editing.

10 affect cis-regulatory elements to alter RNA editing.

11 isogenic iPSC lines using CRISPR/Cas9 genome editing.

12 indromic repeats (CRISPR-Cas9)-mediated gene editing.

13 , were designed for site specific epigenetic editing.

14 s been shown to inhibit adenosine-to-inosine editing.

15 ADAR3 predominantly acts as an inhibitor of editing.

16 nding of the biological importance of A-to-I editing.

17 of synthetic donor DNAs for efficient genome editing.

18 n of target genes undergoing extensive 3'UTR editing.

19 cell stage, but become Env(-) upon receptor editing.

20 ay depending on the occurrence and extent of editing.

21 number of sites that can be targeted by base editing 2.5-fold.

22 RNA editing, a post-transcriptional process, allows the dive

23 erent ADAR1 binding behaviors related to its editing activity, as well as the antagonizing effect of

24 nocarriers delivering mRNA encoding a genome-editing agent can efficiently knock-out selected genes i

25 and structural requirements of pre-miRNA for editing along with a suggestive crucial role for ADAR2.

26 ly edited transcripts within it to show that editing alters gene expression by modulating translation

27 scripts that are targets of editing and that editing alters their function.

28 tudy reveals widespread cis variation in RNA editing among genetically distinct individuals and sheds

29 ndrial mRNAs undergo internal changes by RNA editing and 3' end modifications.

30 inhibition using CRISPR/Cas-mediated genome editing and demonstrate that KRAS is dispensable in a su

31 ammable meganucleases is transforming genome editing and functional genomics.

32 r and have important implications for genome editing and genome evolution.

33 ry, SNGD promotes precise and efficient gene editing and may be a promising strategy for the developm

34 based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-derivatives in se

35 enriched for transcripts that are targets of editing and that editing alters their function.

36 ags as fusion partners in Cas9-mediated gene editing and the construction of doubly DNA-tethered prot

37 as been widely adopted for RNA-guided genome editing and transcription regulation in applications suc

38 Finally, genome-editing and transgenic approaches demonstrate that a hig

39 Here we implement CRISPR-Cas9 genome editing and transposon-mediated somatic gene transfer to

40 cope of applications in nanotechnology, gene editing, and DNA library construction.

41 Advances in chemical genomics, gene editing, and model systems now permit deconstruction of

42 Adaptation of CRISPR-Cas9 for genome-editing applications has revolutionized biomedical resea

43 he time and cost of in vitro or ex vivo gene-editing applications in precision medicine and drug disc

44 ian cells and are thus precluded from genome-editing applications.

45 powerful tools for gene transfer and genome editing applications.

46 This genome editing approach has the advantage that it does not requ

47 Here we report a genome editing approach in which adeno-associated virus (AAV)-m

48 reporter assays, we sought to develop a gene editing approach to investigate the regulatory activity

49 We developed a genome-editing approach to target a dominantly inherited form o

50 , CRISPR-Cas9 tools for site-specific genome editing are needed to facilitate further improvements in

51 ese mutant collections, together with genome editing, are being used in polyploid species to combine

52 player in memory and establishes epigenetic editing as a potential therapy to treat human neurologic

53 numerous sites of insertion versus deletion editing as editosomes collaborate to accurately edit tho

54 The rate of miR487b editing, as well as 2'-O-ribose-methylation, is increase

55 38 mitochondrial editing sites and increased editing at 24 sites; therefore the absence of MEF8 affec

56 -DNA insertion (mef8) line exhibited reduced editing at 38 mitochondrial editing sites and increased

57 Here the authors show, using CRISPR gene editing, ATAC-seq and ChIP-seq, that specific Runx1-boun

58 This study provides a genome editing-based multiplex strategy for direct functional i

59 n for its promise in basic research and gene editing-based therapeutics.

60 ding inefficiencies in targeted nuclear gene editing broadly hinder Chlamydomonas research.

61 for efficient sporulation and suggests that editing by aminoacyl-tRNA synthetases may be important f

62 key features of current knowledge of genomic editing by CRISPR/Cas9 technology as a feasible strategy

63 s, such as Cas9, are used for precise genome editing by homology-dependent repair (HDR).

64 First, we greatly reduce off-target base editing by installing mutations into our third-generatio

65 f precursor RNAs via trans-splicing, and RNA editing by substitution and uridine additions both recon

66 vides insights into the mechanism of peptide editing by TAPBPR and, by analogy, tapasin.

67 ble of robust knockdown and demonstrated RNA editing by using catalytically inactive Cas13 (dCas13) t

68 Finally, we show how inducible gene editing can be achieved by combining the TAEL and CRISPR

69 e transformants demonstrate that plastid RNA editing can be bypassed through the expression of nucleu

70 CRISPR/Cas9-based genome editing can easily generate knockout mouse models by dis

71 Apolipoprotein B mRNA-editing catalytic polypeptide (APOBEC) 3 proteins have b

72 r, we demonstrate that apolipoprotein B mRNA-editing catalytic polypeptide 3 expression and editing f

73 , we demonstrated that apolipoprotein B mRNA-editing catalytic polypeptide 3A (A3A) and A3G expressio

74 Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by Adenosine DeAminases acting on dou

75 Ablation of PheRS editing caused accumulation of Tyr-tRNAPhe (5%), but not

76 ion, protein replacement therapy, and genome editing, collectively affecting approaches for the preve

77 biquitin protein ligase (ITCH)-A20 ubiquitin-editing complex inhibits receptor-interacting Ser/Thr ki

78 ly used in other lab animals to deliver gene editing constructs have been less effective in songbirds

79 metabolic disease modeling by somatic genome editing could be applied to many other systemic as well

80 ggest that TCR transfer combined with genome editing could lead to new, improved generations of cance

81 An editing defect, leading to an amino acid change, in the

82 is of these complemented plants showed major editing defects in both organelles with a very high PPR

83 and DYW2-GFP overexpressing lines show broad editing defects in both organelles, with predominant spe

84 fficiency could however not be linked to any editing defects in the nad2 transcript.

85 The editing-dependent stabilization of mRNAs in turn alters

86 In light of recent human embryo editing developments, scientists and stakeholders from a

87 CRISPR/Cas9 is a promising tool for genome-editing DNA in cells with single-base-pair precision, wh

88 ually synthesized with associated acyl chain editing during nitrogen stress, in contrast to an overal

89 g mutations and context-dependence of genome editing efficiency that would confound other strategies.

90 plasmid backbone markedly improved the gene-editing efficiency.

91 We showed that CRIPSR-mediated genome editing efficiently excised the mutant exon 23 in dystro

92 62 in response to shRNA knockdown of the RNA editing enzyme ADAR1.

93 antibody gene deaminase AID and the RNA/DNA editing enzyme APOBEC1 (A1).

94 The apolipoprotein B mRNA editing enzyme catalytic polypeptide-like APOBEC3A and A

95 Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) prot

96 ble software, SAILOR, identified 7361 A-to-I editing events across the neural transcriptome.

97 The majority of editing events alter the sequence of the 3'UTR of target

98 Tens of thousands of A-to-I editing events are defined in the mouse, yet the functio

99 transcriptomes, cancer-specific recoding RNA editing events have yet to be discovered.

100 e polymorphisms (SNPs) in the genome, or RNA editing events within the RNA.

101 have reported key roles for individual miRNA editing events, but a comprehensive picture of miRNA edi

102 logy; functional and comparative OMICs; gene editing; expanded use of model organisms; and a new sing

103 Interestingly, gene silencing or editing experiments revealed that SNAT7 is the primary p

104 matR transcripts' sites showed a decrease of editing extent in the mef8 mutant.

105 tidial CHLOROPLAST BIOGENESIS 19 (CLB19) PPR editing factor.

106 e protein interactions between the different editing factors are still poorly understood.

107 iting catalytic polypeptide 3 expression and editing function was heat sensitive to a certain degree,

108 isacylation through a separate post-transfer editing function.

109 CRISPR/Cas9 genome editing generated predicted null mutations in cnrip1a an

110 ed as a sequence-specific molecular tool for editing genomic sequences for basic research in life sci

111 The revolution in CRISPR-mediated genome editing has enabled the mutation and insertion of virtua

112 The introduction of Cas9-directed genome editing has expanded adoption of this approach.

113 Genome editing has potential for the targeted correction of ger

114 Although Cas9-mediated genome editing has proven to be a powerful genetic tool in euka

115 trate that AAV-mediated muscle-specific gene editing has significant potential for therapy of neuromu

116 high-throughput omics profiling, and genome editing, have begun to elucidate plant terpene metabolis

117 Focusing on the RNA editing hotspot in miR-200b, a key tumor metastasis supp

118 /CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness u

119 g of psbF-C77 and the reduction in accD-C794 editing in Arabidopsis (Arabidopsis thaliana).

120 allows the rapid detection of iSTOP-mediated editing in cell populations and clones.

121 Despite the demonstration of gene editing in Chlamydomonas in 1995, the isolation of mutan

122 The degree of differential RNA editing in epileptic mice correlated with frequency of s

123 time to establish mutant lines, mosaic gene editing in founder animals, and low homologous recombina

124 Our study highlights the importance of miRNA editing in gene regulation and suggests its potential as

125 events, but a comprehensive picture of miRNA editing in human cancers remains largely unexplored.

126 We utilized CRISPR/Cas9 genome editing in human induced pluripotent stem (iPS) cell-der

127 d the accessibility and efficiency of genome editing in many organisms.

128 Like bone marrow-derived macrophages, RNA editing in MG leads to overall changes in the abundance

129 Editing in microRNAs, particularly in seed can significa

130 educe hypoxia-induced gene expression or RNA editing in monocytes.

131 To establish CRISPR-directed gene editing in N. vitripennis, we targeted a conserved eye p

132 Gang et al. report CRISPR/Cas9 genome editing in parasites of the genus Strongyloides, generat

133 ker and allows sophisticated markerless gene editing in situ.

134 t reports suggesting increased levels of RNA editing in squids thus raise the question of the nature

135 essed their utility for site-specific genome editing in two insect cell lines commonly used as hosts

136 sibility of CRISPR/Cas9-based cardiac genome editing in vivo in postnatal mice.

137 and serial dosing regimens for somatic gene editing in vivo.

138 live mice to achieve specific, DNA-free base editing in vivo.

139 r techniques can produce high frequency gene editing in X. laevis, permitting analysis in the F0 gene

140 SLA class I is a target for genome editing in xenotransplantation.

141 the authors show high efficient single-base editing in zebrafish using modified Cas9 and its VQR var

142 ated stabilization of Ctn RNA occurred in an editing-independent manner.

143 Base editing induces single-nucleotide changes in the DNA of

144 luding gene overexpression, CRISPR/Cas9 gene editing, inducible technologies, optogenetic or DREADD c

145 ADAR3) as an important regulator of Q/R site editing, investigate its mode of action, and detect elev

146 Adenosine-to-inosine (A-to-I) RNA editing is a conserved post-transcriptional mechanism me

147 We conclude that CRISPR-Cas9-mediated genome editing is a powerful method for investigating gene func

148 Targeted epigenome editing is an emerging technology to specifically regula

149 RNA editing is an essential post-transcriptional process tha

150 Editing is enriched in the nervous system, affecting mol

151 The application of base editing is limited by off-target activity and reliance o

152 However, R/G editing is only effective in flop channels.

153 We here show that RNA editing is particularly common in behaviorally sophistic

154 Large-scale studies have revealed how editing is regulated both in cis and in trans.

155 t to which most sites are edited and how the editing is regulated in different biological contexts ar

156 t for few mammalian conserved editing sites, editing is significantly higher in neurons than in other

157 While the primary role of aaRS editing is to prevent misaminoacylation, we demonstrate

158 monstrate that the lifetime absence of ADAR1-editing is well tolerated in the absence of MDA5.

159 SNGD-mediated gene editing led to a markedly lower indel frequency than tha

160 tasis suppressor, we found that the miR-200b editing level correlates with patient prognosis opposite

161 o demonstrate that while temperature affects editing levels at more sites than genetic differences, g

162 Further, NUP43 exhibits constant editing levels between single cells, while GRIA2 editing

163 We show that editing levels in non-repetitive coding regions vary mor

164 oding regions vary more between tissues than editing levels in repetitive regions.

165 ization of mRNAs in turn alters the observed editing levels in the stable RNA repertoire.

166 ing levels between single cells, while GRIA2 editing levels vary.

167 at an intronic SNP in prominin regulates its editing levels.

168 of which are diversified by splicing and RNA editing, localize to >20 excitatory and inhibitory neoco

169 iting damage and simplifying the delivery of editing machinery.

170 found that GeoCas9 is an effective tool for editing mammalian genomes when delivered as a ribonucleo

171 Although RNA editing markedly increases complexity of the cancer cell

172 eles up to 18-fold higher than standard gene-editing methods, and enrich cell populations containing

173 itochondrial PPR protein that is involved in editing nad4, possibly required for the efficient splici

174 , up to 8%, is higher than most other genome editing nucleases, indicative of its effective enzymatic

175 elf-Thy-1 ligand, immunoglobulin light chain editing occurred, generating B cells with up-regulated N

176 Using CRISPR-Cas9 genome editing of bptf in zebrafish to induce a loss of gene fu

177 ell as for adenosine to inosine (A to I) RNA editing of Ctn RNA in muscle cells.

178 acterization as well as detailed interactive editing of filopodia reconstructions through an intuitiv

179 ansduces ECs of pathologic vessels, and that editing of genomic VEGFR2 locus using rAAV1-mediated CRI

180 Genome editing of human induced pluripotent stem cells (hiPSCs)

181 to prevent misaminoacylation, we demonstrate editing of misaminoacylated tRNA is also required for de

182 intravenous injection into mice induces >80% editing of Pcsk9 in the liver.

183 Precise genome editing of plants has the potential to reshape global ag

184 We demonstrate editing of post-mitotic neurons in the adult mouse brain

185 Here, we demonstrate that CRISPR/Cas9 genome editing of promoters generates diverse cis-regulatory al

186 N6 (ORRM6) result in the near absence of RNA editing of psbF-C77 and the reduction in accD-C794 editi

187 nd in cells rendered ISR-deficient by CRISPR editing of the Eif2s1 locus to encode a non-phosphorylat

188 We used CRISPR/Cas9-mediated gene editing of the Ern1 locus to study the role of the TMD i

189 le-restricted Cas9 expression enables direct editing of the mutation, multi-exon deletion or complete

190 man salivary ductal cells through epigenetic editing of the native promoter.

191 Genome editing of the neurokinin 1 receptor (NK1R) in the VTA r

192 e of gene activation/expression and receptor editing of these isotypes have not been studied.

193 d through pharmacologic inhibition or genome editing of these loci.

194 CRISPR/Cas9-based genome editing offers the possibility to knock out almost any g

195 These results indicate that RNA editing on chlB mRNA is important to maintain appropriat

196 g their deaminase recruitment strategies and editing outcomes, and compare them to other CRISPR genom

197 docannabinoids signaling pathway and the RNA editing pathway were found to be dysregulated in EC.

198 sponsible for alterations in the tissue-wide editing patterns upon injury.

199 n engineered single guide RNA (sgRNA) genome editing platform that offers revolutionary solutions to

200 REPAIR presents a promising RNA-editing platform with broad applicability for research,

201 Here, we review the different base-editing platforms, including their deaminase recruitment

202 Our findings suggest miR487b editing plays an intricate role in postischemic neovascu

203 The gene editing potential of CRISPR/Cas9 encapsulated by ZIF-8 (

204 While trans-acting tRNA editing proteins have been found to counteract the misac

205 As genome editing rapidly progresses toward the realization of its

206 an genome is challenging with non-viral gene-editing reagents, since most of the edited sequences con

207 Base editing relies on recruitment of cytidine deaminases to

208 n zygotes, its application in postnatal gene editing remains incompletely characterized.

209 th DNA damage response and modulation of DNA editing/repair gene expression.

210 Overall, single TCR gene editing represents a clinically feasible approach that i

211 Mechanistically, SNGD-mediated gene editing requires long-sequence homology between the targ

212 Loss of CXCR7 expression by CRISPR-Cas9 gene editing resulted in a halt of cell proliferation, severe

213 orter gene assays, we could demonstrate that editing results in a complete switch of target site sele

214 In vivo genome editing results in lower IOP and prevents further glauco

215 Epigenomic profiling and genome editing revealed that AMIGO2 is regulated by a melanoma-

216 CRISPR-Cas9 gene editing revealed that both BTK and B lymphocyte kinase (

217 ines have been repurposed to enable a genome editing revolution.

218 (for example, mutagenesis, CRISPR-based gene editing, RNA interference, morpholinos or pharmacologica

219 a novel and clinically feasible TCR "single editing" (SE) approach, based on the disruption of the e

220 xhibited reduced editing at 38 mitochondrial editing sites and increased editing at 24 sites; therefo

221 Although many editing sites have recently been discovered, the extent

222 However, the vast majority of these RNA editing sites have unknown functions and are in noncodin

223 ate to accurately edit thousands of distinct editing sites in vivo.

224 The genomic sequence flanking editing sites is highly conserved, suggesting that the p

225 und that, except for few mammalian conserved editing sites, editing is significantly higher in neuron

226 because of the unknown function of most RNA editing sites.

227 le further optimization of Cas9-based genome-editing specificity and efficiency.

228 nd are regulated by post-transcriptional RNA editing, splice variation, post-translational modificati

229 as two antagonistic effects on mitochondrial editing: stimulatory, which requires a catalytic glutama

230 transgenic cells can be used for other gene-editing studies and is well-suited for high-throughput s

231 The CRISPR-Cas9 genome-editing system is a part of the adaptive immune system i

232 efficient delivery of the CRISPR-Cas9 genome-editing system to target cells in human body.

233 Here, we report a programmable "base editing" system to induce precise base conversion with h

234 evasion of one but not both tRNA synthetase editing systems.

235 shown to be an efficient and accurate genome-editing technique.

236 Then, we discuss how genome editing techniques enable a radically new set of approac

237 , induced mutations, and the advanced genome-editing technologies can be applied to improving the nut

238 the powerful combination of iPSCs and genome editing technologies for understanding the biological fu

239 Gene-editing technologies have made it feasible to create non

240 Recent advancement in genome editing technologies offers a promising therapeutic appr

241 mes, and compare them to other CRISPR genome-editing technologies.

242 ce of human biomaterials and the use of gene-editing technologies.

243 Using gene editing technology (CRISPR/Cas9), the SIRT1 gene was rem

244 ements and broad adoption of the Cpf1 genome editing technology have the potential to make a dramatic

245 We developed the TCR gene editing technology that is based on the knockout of the

246 b2 in neural development, we utilized genome-editing technology to generate an allelic series in the

247 Here the authors employ CRISPR/Cas9 gene editing technology to silence VEGFR2, a major regulator

248 CRISPR-Cas9 is a genome editing technology with major impact in life sciences.

249 CRISPR/Cas is a revolutionary gene editing technology with wide-ranging utility.

250 e of mutations generated by CRISPR/Cas9 gene-editing technology, and alleles designed to be null can

251 CRISPR-Cas9 has become a facile genome editing technology, yet the structural and mechanistic f

252 s/CRISPR-associated gene9 (CRISPR/Cas9) gene editing technology.

253 it is inappropriate to perform germline gene editing that culminates in human pregnancy.

254 We developed a strategy for precise gene editing that does not generate DSBs.

255 equencing, genetic complementation, and gene editing, that haploid induction in maize (Zea mays) is t

256 quence truncation procedure is then used for editing the models based on local variations of the stru

257 nated regulation of organellar multiple site editing through DYW2, which probably provides the deamin

258 lating centrosome activities, we used genome editing to ablate it.

259 derscore the potential of CRISPR/Cas9 genome editing to advance immunotherapies.

260 at were engineered by CRISPR-mediated genome editing to controllably release GLP-1 (glucagon-like pep

261 Here we used CrispR-Cas9-mediated gene editing to delete the gene encoding for AC, ASAH1, in hu

262 Here we applied CRISPR-Cas9 genome editing to disrupt the endogenous human MRP RNA locus, t

263 Here we use CRISPR-Cas9-mediated genome editing to investigate the function of the pluripotency

264 e adeno-associated virus (AAV)-mediated gene editing to knock in HLA-E genes at the B2M locus in huma

265 In this study, we used genome editing to knockout the two mcoln1 genes present in Dani

266 Towards this end, we used CRISPR-Cas9 genome editing to make a single allele knock-in of the most com

267 -electrochemistry and site-selective isotope editing to monitor the CO/CN(-) stretching vibrations in

268 ompelling demonstration of the power of gene editing to rapidly improve yield traits in crop breeding

269 , we apply the approach of site-directed RNA editing to repair, at the mRNA level, a disease-causing

270 Here we used CRISPR/Cas9 genome editing to separate catalytic activity-dependent and ind

271 With CRISPR/Cas9-mediated gene editing to stably knock out and recover Rab8a in macroph

272 Here we apply CRISPR-Cas9 gene editing to tag a cytoskeletal protein (alpha-tubulin) an

273 he CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to recruitment of

274 harnessed as a powerful and versatile genome-editing tool and holds immense promise for future therap

275 suggest that CRISPR/Cas9 is a powerful gene editing tool that can uncover novel mechanisms of cluste

276 CRISPR is a versatile gene editing tool which has revolutionized genetic research i

277 s question, we use a CRISPR-dCas9 epigenetic editing tool, where an inactive form of Cas9 is fused to

278 nd plasmids, as well as a revolutionary gene editing tool.

279 tors provide an organized comprehensive gene editing toolbox of considerable scientific value.

280 the capabilities of the RNP-mediated genome editing toolbox.

281 Here, we employ diverse CRISPR/Cas9 genome editing tools to generate a series of targeted lesions w

282 teocytic cell lines-together with new genome editing tools-has allowed a closer look at the biology a

283 of postnatal CRISPR/Cas9-based cardiac gene editing using adeno-associated virus serotype 9 to deliv

284 tein expressions as well as single-cell gene editing using clustered regularly interspaced short pali

285 ic-transfection technique for precise genome editing using CRISPR-Cas9.

286 on was corrected by CRISPR/Cas9-based genome editing (V247fs-MT-correction).

287 The advantages of gene editing versus gene targeting in embryonic stem cells, i

288 Precise genome editing via homology-directed repair (HDR) in targeted c

289 Nicotiana benthamiana (16c) plants, and gene editing was accompanied by loss of GFP expression.

290 CRISPR/Cas9 gene editing was used to knock out pig conceptus IL1B2 expres

291 vage is a likely point of regulation for RNA editing, we elucidated endonuclease specificity in vivo.

292 HIV provirus gene-editing were confirmed by cell genomic DNA PCR and fluor

293 and "seizures." Genes with differential RNA editing were preferentially enriched for genes with a ge

294 rominently reduces the workload of cell-line editing, which may be completed within 4 weeks.

295 aminase domains that narrow the width of the editing window from approximately 5 nucleotides to as li

296 ZNP delivery of sgRNA enables permanent DNA editing with an indefinitely sustained 95 % decrease in

297 Here we combine CRISPR/Cas9 gene editing with an innovative high-throughput genotyping pi

298 Gene editing with engineered nucleases enables site-specific

299 iew this RNA-guided nuclease system for gene editing with respect to its usefulness for cardiovascula

300 cola, allowed efficient Cas9-mediated genome editing without the need for a repair template.