ライフサイエンスコーパス: egg extract

1 immobilized in agarose prior to addition of egg extract.

2 cell-free system based on interphase Xenopus egg extract.

3 embly of anastral spindles in Xenopus laevis egg extract.

4 is Ran- and importin alpha-dependent in the egg extract.

5 tabilization and Axin degradation in Xenopus egg extract.

6 ction partner of SUMOylated PARP1 in Xenopus egg extract.

7 are defective for DNA replication in Xenopus egg extracts.

8 S50/T64/T68/T79/S114/S165) by CDK in Xenopus egg extracts.

9 n-coated beads after introduction of Xenopus egg extracts.

10 using Ran-mediated asters in meiotic Xenopus egg extracts.

11 ation protein complexes from Xenopus meiotic egg extracts.

12 th strand-specific DNA roadblocks in Xenopus egg extracts.

13 tion that occurs at the MBT is reproduced in egg extracts.

14 n with PP1 is disrupted in mitotic cells and egg extracts.

15 dent kinase (Cdk) activity in Xenopus laevis egg extracts.

16 ld increase of Lis1 concentration in Xenopus egg extracts.

17 res of meiotic spindles assembled in Xenopus egg extracts.

18 essential for mitotic SUMOylation in Xenopus egg extracts.

19 skewer metaphase spindles in Xenopus laevis egg extracts.

20 ules, to support spindle assembly in Xenopus egg extracts.

21 eslin that associates with TopBP1 in Xenopus egg extracts.

22 y in response to oxidative stress in Xenopus egg extracts.

23 ontaining Cdc7, Drf1, and Claspin in Xenopus egg extracts.

24 the ATR-activating protein TopBP1 in Xenopus egg extracts.

25 interaction zones between asters in Xenopus egg extracts.

26 necessary for spindle bipolarity in Xenopus egg extracts.

27 and nonspindle assemblies, in Xenopus laevis egg extracts.

28 pulation of preassembled spindles in Xenopus egg extracts.

29 depends upon ATM in human cells and Xenopus egg extracts.

30 for M phase entry and maintenance in Xenopus egg extracts.

31 fusion and nuclear pore assembly in Xenopus egg extracts.

32 othesis with model DNA substrates in Xenopus egg extracts.

33 tebrate meiotic spindle assembled in Xenopus egg extracts.

34 for a model mitotic CDK substrate in Xenopus egg extracts.

35 alcium-induced exit from metaphase arrest in egg extracts.

36 ocalization, and spindle assembly in Xenopus egg extracts.

37 he model were tested and verified in Xenopus egg extracts.

38 chondrial enzyme, from HeLa cell and Xenopus egg extracts.

39 he Rad9-Hus1-Rad1 (9-1-1) complex in Xenopus egg extracts.

40 ATR-dependent checkpoint pathway in Xenopus egg extracts.

41 extracts employing Xenopus laevis oocytes or egg extracts.

42 fective inhibitor of checkpoint signaling in egg extracts.

43 nd ATR in a DNA damage checkpoint in Xenopus egg extracts.

44 ounterpart when incubated in Xenopus M phase egg extracts.

45 MAPK phosphorylates Mps1 at S844 in Xenopus egg extracts.

46 unction, we examined its behavior in Xenopus egg extracts.

47 induce Op18 hyperphosphorylation in Xenopus egg extracts.

48 d physiological spindle assembly activity in egg extracts.

49 to stalled DNA replication forks in Xenopus egg extracts.

50 yte maturation and during mitosis in Xenopus egg extracts.

51 mics, and is the major catastrophe factor in egg extracts.

52 specific replication fork pausing in Xenopus egg extracts.

53 in tissue culture cells but not in X. laevis egg extracts.

54 activity present in Xenopus M phase-arrested egg extracts.

55 ked anaphase sister chromatid segregation in egg extracts.

56 on one another for stable binding to DNA in egg extracts.

57 ior to nuclear envelope breakdown in cycling egg extracts.

58 fications on chromosomal replication in frog egg extracts.

59 kinases together promotes its degradation in egg extracts.

60 len- and abasic site-induced ICLs in Xenopus egg extracts.

61 slation, leads to spindle defects in Xenopus egg extracts.

62 omes with a site-specific barrier in Xenopus egg extracts.

63 during perturbed DNA replication in Xenopus egg extracts.

64 ryonic isoform of linker histone H1 (H1M) in egg extracts.

65 inetochore-microtubule attachment in Xenopus egg extracts.

66 h zero-background interference to be made in egg extracts.

67 o, and loads Cdc45 onto chromatin in Xenopus egg extracts.

68 hase chromosome alignment defects in Xenopus egg extracts.

69 endent but ATM-independent manner in Xenopus egg extracts.

70 ned ATR-activating DNA structures in Xenopus egg extracts.

71 using crude and fractionated Xenopus laevis egg extracts.

72 spindle assembly around DNA-coated beads in egg extracts.

73 totic chromosomes in human cells and Xenopus egg extracts.

74 of existing microtubules in meiotic Xenopus egg extracts.

75 ffer or during chromatin assembly in Xenopus egg extracts.

76 d it to replication-competent Xenopus laevis egg extracts.

77 etaphase spindle assembled in Xenopus laevis egg extracts.

78 In egg extracts, a configuration in which outer kinetochore

79 Immunodepletion of Xnf7 from Xenopus laevis egg extracts accelerated the degradation of APC substrat

80 55 delta (in interphase) from 'cycling' frog egg extracts accelerated their entry into mitosis and ke

81 In egg extracts, activation of Chk1 checkpoint kinase requi

82 Our results show that in Xenopus egg extracts, aggregation of multiple replication forks

83 In egg extracts alanine mutation of the DUE-B C-terminal ph

84 breaks and stalled replication forks in both egg extract and human cells, specifically colocalizing w

85 -3-3zeta dissociation from caspase-2 in both egg extract and human cultured cells.

86 bly of spindle and spindle matrix in Xenopus egg extract and in mammalian cells.

87 We describe an in vitro system using Xenopus egg extract and purified centrioles in which both centri

88 Through experiments in egg extract and reconstitution with purified proteins, w

89 Additional experiments in Xenopus egg extracts and artificially crowded in vitro solutions

90 s associate with B-Raf at mitosis in Xenopus egg extracts and contribute to its phosphorylation.

91 uclei and metaphase spindles both in Xenopus egg extracts and cultured cells.

92 for mitotic chromosomal SUMOylation in frog egg extracts and demonstrated that it can mediate effect

93 the initiation of DNA replication in Xenopus egg extracts and during early embryonic development.

94 P2A:B55delta), during mitotic cycles in frog-egg extracts and early embryos.

95 d instead functions to enhance H1 binding in egg extracts and embryos.

96 e significantly reduced PARP1 SUMOylation in egg extracts and enhanced the accumulation of species de

97 e previously studied this process in Xenopus egg extracts and established Greatwall (Gwl) as an impor

98 m-mediated spindle assembly assay in Xenopus egg extracts and extensive mutagenesis studies, we have

99 tudy the metaphase spindle in Xenopus laevis egg extracts and found that microtubules are shortest ne

100 that acts on model CDK substrates in Xenopus egg extracts and has antimitotic activity.

101 lated to S. pombe Cdt2, functions in Xenopus egg extracts and human cells to destroy the replication

102 Using Xenopus egg extracts and human cells, we show that the tumor sup

103 dk for this interaction using both X. laevis egg extracts and human cells.

104 n of ATR-dependent signaling in both Xenopus egg extracts and human cells.

105 1N is unable to support resection in Xenopus egg extracts and human cells.

106 epletion of PP1 impairs NHEJ in both Xenopus egg extracts and human cells.

107 Here, using Xenopus egg extracts and human somatic cells, we show that actin

108 s DSB-induced ATM activation in both Xenopus egg extracts and human tumor cell lines.

109 adducts were efficiently resected in Xenopus egg extracts and immunodepletion of Xenopus DNA2 also st

110 these oocytes and also causes CSF arrest in egg extracts and in blastomeres of two-cell embryos.

111 Previous studies in Xenopus laevis egg extracts and in highly proliferative cells showed th

112 Using Xenopus egg extracts and in vitro assays, we show that the Xenop

113 PIASy promotes SUMOylation of PARP1 both in egg extracts and in vitro reconstituted SUMOylation assa

114 rates the mitotic G2/M transition in cycling egg extracts and induces meiotic maturation in G2-arrest

115 ation decreases severing activity in Xenopus egg extracts and is involved in controlling spindle leng

116 Addition of the Mre11 inhibitor mirin to egg extracts and mammalian cells reduces RCC1 associatio

117 duced S-phase checkpoint response in Xenopus egg extracts and mammalian cells.

118 Here, using Xenopus egg extracts and mass spectrometry, we identify SMARCAL1

119 ion from both interphase and mitotic Xenopus egg extracts and mass spectrometry.

120 ules during metaphase in both Xenopus laevis egg extracts and mitotic human cell extracts.

121 By combining studies in Xenopus laevis egg extracts and mouse embryonic fibroblasts (MEFs), we

122 fies chromosomal proteins in mitotic Xenopus egg extracts and plays an essential role in mitotic chro

123 oci or a single chromosomal locus in Xenopus egg extracts and show that a complex library can target

124 f the MAPK cascade during mitosis in Xenopus egg extracts and showed that B-Raf activation is regulat

125 studied assembly of chromatin using Xenopus egg extracts and single DNA molecules held at constant t

126 ited to a DSB-mimicking substrate in Xenopus egg extracts and sites of laser microirradiation in huma

127 ink between DNA replication and N/C ratio in egg extracts and suggest a mechanism that may influence

128 smin binds SENP3 and SENP5 in Xenopus laevis egg extracts and that it is essential for stable accumul

129 role in mitotic spindle assembly in Xenopus egg extracts and that this role is independent of cyclin

130 reconstruction microscopy (STORM) to Xenopus egg extracts and tissue culture cells, we report various

131 ic chromosome architecture in Xenopus laevis egg extracts and, unlike core histones, exhibits rapid t

132 In Xenopus egg extracts And-1 is loaded on the chromatin after Mcm1

133 laevis and human tissue culture cells, frog egg extracts, and budding yeast.

134 s transiently activated after adding Ca2+ to egg extracts, and inhibitors of calcineurin such as cycl

135 branous spindle matrix isolated from Xenopus egg extracts, and it is required for proper spindle morp

136 RIR of a site-specific ICL lesion in Xenopus egg extracts, and that both its catalytic activity and U

137 BubR1 associates with APC and EB1 in egg extracts, and the complex formation is necessary for

138 reveals that microtubule dynamics in Xenopus egg extracts are unaffected by maskin depletion.

139 tic activation, using cyclin-treated Xenopus egg extracts as a model system, and presented evidence t

140 Using Xenopus egg extracts as a vertebrate model system, we showed pre

141 We have used Xenopus egg extracts as an in vitro system to study the role of

142 In Xenopus egg extract assays, we showed that poly(ADP-ribose) poly

143 In Xenopus egg extracts, ATM associates with TopBP1 and thereupon p

144 ng assay wherein nuclei assembled in Xenopus egg extract become smaller in the presence of cytoplasmi

145 Addition of the LB3T-Ig to egg extracts before the introduction of chromatin preven

146 ow that, in both mammalian cells and Xenopus egg extracts, BRCA1/BARD1 is required for mitotic spindl

147 is required for maintenance of CSF arrest in egg extracts, but its function in CSF establishment in o

148 otes is inferred from data in Xenopus laevis egg extracts, but its identity remains elusive.

149 ormation in vivo in activated oocytes and in egg extracts, but not in immature or in vitro matured oo

150 ay is suppressed in the cytoplasm of Xenopus egg extract by phosphatases, but that it becomes activat

151 othesis, the induction of mitosis in Xenopus egg extracts by the addition of cyclin B was blocked by

152 dk2, and sustained cyclin E-Cdk2 activity in egg extracts causes metaphase arrest in the absence of M

153 Here, we report that in Xenopus egg extracts, Cdc7-Drf1 is far more abundant than Cdc7-D

154 Further, in egg extracts, Claspin phosphorylation depends on a thres

155 study, graded concentrations of herring gull egg extracts, collected from five Great Lakes breeding c

156 N-APC interacts with Mad2 in Xenopus egg extracts, colon cancer cells, and in vitro with puri

157 Experiments in frog egg extract confirm the main theoretical predictions.

158 Xenopus egg extracts containing a mutant of TopBP1 that cannot b

159 Furthermore, Xenopus egg extracts containing a version of TopBP1 with an inac

160 ys increased binding to ATR-ATRIP in Xenopus egg extracts containing checkpoint-inducing DNA template

161 Egg extracts containing either a mutant of TopBP1 lackin

162 ATR-dependent activation of Chk1 in Xenopus egg extracts containing incompletely replicated DNA.

163 c RCC1/Ran/RanBP1 complex in M phase Xenopus egg extracts controls both RCC1's enzymatic activity and

164 XNercc endogenous to meiotic egg extracts coprecipitates a multiprotein complex that

165 In egg extracts, CSF release by calcium was inhibited by co

166 ahymena group I ribozyme embedded in Xenopus egg extract demonstrate the ability of M2-seq to detect

167 Studies in Xenopus egg extracts demonstrate that Repo-Man interacts with AT

168 analysis using human DR-GFP cells or Xenopus egg extract demonstrated that MCM8 and MCM9 proteins are

169 Polarization microscopy in "cycling" egg extracts demonstrates that de novo centriole formati

170 urther characterization of Pontin in Xenopus egg extracts demonstrates that Pontin interacts with the

171 ear scaling was recapitulated in vitro using egg extracts, demonstrating that titratable cytoplasmic

172 hromosomes accumulate DSBs in Xenopus laevis egg extracts depleted of ATM and ATR.

173 In addition, treatment with egg extract did not enhance expression or stability of J

174 wever, depletion of the protein from cycling egg extracts does not prevent mitotic cell cycle progres

175 During replication in Xenopus egg extracts, DUE-B and Cdc45 bind to chromatin with sim

176 Soluble Xenopus egg extracts efficiently replicate added plasmids using

177 The signal persists in egg extracts even after damaged DNA is removed from the

178 nuclear expansion, in Dppa2-depleted Xenopus egg extracts excess microtubules cause pronuclear assemb

179 pletion of Greatwall kinase prevents Xenopus egg extracts from entering or maintaining M phase due to

180 ton grazer, Daphnia pulicaria, using dormant eggs extracted from sediments in two Minnesota lakes (So

181 Xenopus egg extracts have distinct Cdk-active and Cdk-inactive s

182 h human cells and in vitro data with Xenopus egg extracts have led to the conclusion that the kinase

183 ed illumination microscopy to Xenopus laevis egg extracts, here we reveal that in the absence of micr

184 In Xenopus egg extracts, ICL repair is initiated when two replicati

185 Consistently, in Xenopus egg extracts, Idas-Geminin is less active in licensing i

186 Immunodepletion of DDK from Xenopus egg extracts impairs chromatin association of Scc2-Scc4,

187 dividing, unperturbed embryos and cell-free egg extract in the absence and presence of DNA damage an

188 s with the Mre11-Rad50-Nbs1 (MRN) complex in egg extracts in a checkpoint-regulated manner.

189 tivity can be induced in Xenopus oocytes and egg extracts in the absence of MAPK or Cdc2 activity.

190 Addition of such an N-APC mutant of egg extracts inactivates the mitotic checkpoint.

191 askin interacts with a number of proteins in egg extracts, including XMAP215, a known modulator of mi

192 reconstitution experiments in Xenopus laevis egg extracts indicate that NCOA4 acts as an inhibitor of

193 Immunodepletion from Xenopus egg extracts indicated that both proteins are only found

194 Overexpression of Pnuts in Xenopus egg extracts inhibited both mitotic and meiotic exit.

195 Depletion of the XCdc7/Drf1 from egg extracts inhibited DNA replication, whereas depletio

196 onse to stalled replication forks in Xenopus egg extracts involves a complex pathway containing ATM a

197 Mitotic spindle assembly in Xenopus egg extracts is regulated at least in part by importin b

198 Depletion from human cells or Xenopus egg extracts is used to demonstrate that the ZW10 comple

199 ase-2, the initiator of apoptosis in Xenopus egg extracts, is associated with an accumulation of LCFA

200 We have observed that egg extracts lacking the Mre11-Rad50-Nbs1 (MRN) complex

201 6P) through the pentose phosphate pathway in egg extracts maintains NADPH levels and calcium/calmodul

202 zation of microtubule flux in Xenopus laevis egg extract meiotic spindles.

203 glass-slide and coverslip in a Xenopus frog egg extract motility assay.

204 Using Xenopus egg extracts, nuclei have been assembled and then induce

205 Binding of Treslin to chromatin in egg extracts occurs independently of TopBP1.

206 fferences between meiotic spindles formed in egg extracts of two frog species.

207 ld generate the shorter spindles observed in egg extracts of X. tropicalis compared to X. laevis.

208 mRNAs copurify with mitotic microtubules in egg extracts of Xenopus laevis.

209 Significantly, removal of Claspin from egg extracts only partially abrogates the activation of

210 Depletion of Bub1 from Xenopus laevis egg extract or from HeLa cells resulted in both destabil

211 Addition of purified Ddk to Xenopus egg extracts or overexpression of Dbf4 in HeLa cells dow

212 ly and organization of microtubule arrays in egg extracts, our studies suggest that Pontin has a mito

213 eflection fluorescent microscopy and Xenopus egg extracts, Petry et al. demonstrate that new microtub

214 ecombinant RSK and endogenous RSK in Xenopus egg extracts phosphorylate all three isoforms of human C

215 critical for H3T3 phosphorylation in Xenopus egg extracts, Plk1 and Aurora B both promote this modifi

216 TM and Aven overexpressed in cycling Xenopus egg extracts prevented mitotic entry and induced phospho

217 Immunodepletion of CENP-C from metaphase egg extract prevents kinetochore formation on sperm chro

218 Imaging of single filaments in Xenopus egg extract provided evidence that disassembly by bursti

219 In Xenopus egg extracts, recombinant L also inhibits mitotic spindl

220 We show that X. tropicalis egg extracts reconstitute the fundamental cell cycle eve

221 After incubation in egg extract, reconstituted CENP-A chromatin specifically

222 Using a Xenopus laevis egg extract replication system, we previously demonstrat

223 timulated to initiate replication in Xenopus egg extracts, replication initiated without any detectab

224 Maskin depletion from egg extracts results in compromised microtubule asters a

225 Immunodepletion of Pnuts from egg extracts revealed its essential functions in mitotic

226 ly embryo equivalent (pronuclei incubated in egg extract), S3 neurula cells, A6 kidney cells, and ery

227 he major Th2-inducing component from soluble egg extract (SEA) as the secreted T2 ribonuclease, omega

228 Egg extracts should be prepared in 1 d and can be stored

229 or of mitotic entry, and new work in Xenopus egg extracts shows that Greatwall is required for the po

230 In egg extracts, single DNA molecules assemble into nucleos

231 Depletion of Treslin from egg extracts strongly inhibits chromosomal DNA replicati

232 idual activities, and how the Xenopus laevis egg extract system has been utilized as a powerful inter

233 nt degradation both in vivo and in a Xenopus egg extract system in vitro.

234 this study, we took advantage of the Xenopus egg extract system to address these questions.

235 Using the Xenopus egg extract system, we have investigated regulation of t

236 ulators, we developed an assay using Xenopus egg extract that recapitulates the activation of transcr

237 ATRIP adopts an oligomeric state in egg extracts that depends upon binding to ATR.

238 However, Claspin-depleted egg extracts that have been reconstituted with these mut

239 magnetic beads and then incubated in Xenopus egg extracts that provide a source for centromere and ki

240 del system using ssDNA templates and Xenopus egg extracts that recapitulates eukaryotic G4 replicatio

241 describe a cell-free system based on Xenopus egg extracts that supports ICL repair.

242 In Xenopus egg extracts, the embryonic linker histone H1M does not

243 Here we demonstrate that in Xenopus laevis egg extracts, the MRN complex is not required for classi

244 In mitotic Xenopus egg extracts, the Nup107-160 complex localized throughou

245 pted to reconstitute this process in Xenopus egg extracts, the only eukaryotic in vitro system that r

246 Upon exposure to Xenopus egg extracts, this DNA underwent extensive replication b

247 In this study we have used Xenopus laevis egg extracts to analyse Uhrf1 function in DNA replicatio

248 ombined microfluidic technology with Xenopus egg extracts to characterize spindle assembly within dis

249 tablished cell-free assays in Xenopus laevis egg extracts to deconstruct the FA pathway in a fully re

250 In this study, we use Xenopus laevis egg extracts to determine the requirements for 9-1-1 loa

251 We have used Xenopus laevis egg extracts to drive an accelerated replication timing

252 We use cell-free Xenopus laevis egg extracts to examine the recruitment of proteins to c

253 In this study, we used Xenopus egg extracts to form spindles in the absence of chromati

254 In this study, we used meiotic egg extracts to gain insight into the role of the Xenopu

255 Here, we have used Xenopus egg extracts to investigate Aur-A's contribution to cell

256 We use Xenopus egg extracts to recapitulate DPC repair in vitro and sho

257 ing assays and functional studies in Xenopus egg extracts to show that TopBP1 makes a direct interact

258 use repair of a site-specific ICL in Xenopus egg extracts to study the mechanism of lesion bypass.

259 ate-related defense responses, we found that egg extract treatment strongly diminished MYC protein le

260 Checkpoint activation occurs in X. laevis egg extracts upon addition of an oligonucleotide duplex

261 Fractionation of the egg extract used for nuclear assembly identified a high

262 re of Listeria actin tails in Xenopus laevis egg extracts using cryo-electron tomography.

263 assembly in tissue culture cells and Xenopus egg extracts using two-photon microscopy with FLIM measu

264 DSB templates that were repaired in Xenopus egg extracts via the canonical, Ku-dependent NHEJ pathwa

265 Here, using Xenopus laevis egg extract we show that MRN-dependent processing of DSB

266 Using Xenopus laevis egg extract we show that Tipin is required for DNA repli

267 Using Xenopus laevis egg extract, we found that increases in cytosolic calciu

268 By using Xenopus laevis egg extract, we found that SUMOylation of DNA topoisomer

269 Using Xenopus laevis egg extract, we have shown that blocking polyubiquitylat

270 Using Xenopus egg extract, we show that direct, cell-cycle-regulated b

271 proteins recruited to DSBs in Xenopus laevis egg extract, we show that DSB-containing DNAs accumulate

272 Using Xenopus egg extracts, we analyzed the functions of FANCM in repl

273 Using Xenopus laevis egg extracts, we demonstrate that Plx1, the Xenopus orth

274 Using Xenopus egg extracts, we describe here a replication-coupled ICL

275 rough biochemical analysis in Xenopus laevis egg extracts, we establish that the MRN (Mre11, Rad50, a

276 Using different assays in Xenopus egg extracts, we found that depleting lamin B caused for

277 MPM-2 epitope kinases in Xenopus oocytes and egg extracts, we have determined that phosphorylation of

278 Using a proteomic screen in Xenopus egg extracts, we identified factors that are enriched on

279 nuclei from transcriptionally silent Xenopus egg extracts, we identified numerous actin regulators, a

280 Using Xenopus egg extracts, we identify an acidic residue in PCNA that

281 Using Xenopus egg extracts, we identify two sequence elements in CRL4(

282 T-Cdc25C phosphorylating activity in Xenopus egg extracts, we previously defined roles of MAPK and Cd

283 from and antibody addition to Xenopus laevis egg extracts, we show that BubR1 and its kinase activity

284 Using Xenopus egg extracts, we show that DNA replication at high N/C r

285 Using Xenopus laevis egg extracts, we show that DNA replication continues at

286 nd spindle assembly assays in Xenopus laevis egg extracts, we show that epsin-induced membrane curvat

287 Working in Xenopus egg extracts, we show that Nudel/NudE facilitates the bi

288 Using X. laevis egg extracts, we show that SSX2IP accumulated at spindle

289 Using Xenopus laevis egg extracts, we show that these excess Mcm2-7 complexes

290 Using Xenopus egg extracts, we show that ultraviolet radiation and aph

291 When Xenopus egg extracts were immunodepleted of Xenopus Hbo1 (XHbo1)

292 nd destroyed in Fizzy/Cdc20-depleted Xenopus egg extracts when only the N-terminal domain of Fizzy/Cd

293 Here, we show in Xenopus laevis egg extract, where the gradient is best characterized, t

294 Depletion of MTBP from Xenopus egg extracts, which also removes Treslin, abolishes DNA

295 longer interact with TopBP1 in Nbs1-depleted egg extracts, which suggests that the MRN complex helps

296 Using Xenopus egg extracts, which support replication-coupled ICL repa

297 e activation, and supplementation of Xenopus egg extract with glucose-6-phosphate, which promotes cas

298 s in tissue culture cells and Xenopus laevis egg extracts with a mathematical model.

299 By incubating frog egg extracts with supported lipid bilayers containing ph

300 Immunodepletion of xCep57 from egg extracts yields weakened and elongated bipolar spind