ライフサイエンスコーパス: electroporate

1 lectroporate, but CEM cells were efficiently electroporated.

2 fraction of the cell's surface area that is electroporated.

3 alculate the approximate current required to electroporate a cell with an electrode of a particular s

4 We injected and electroporated a plasmid encoding ENO1 (or a control pla

5 To examine these events in vivo, we electroporated a soluble Wnt inhibitor or a dominant-neg

6 and a train of voltage pulses applied, which electroporates a nearby cell.

7 ette filled with DNA or other macromolecules electroporates a single cell at the tip of the micropipe

8 By electroporating a cre-recombinase expression vector into

9 E is repressed by GTF3 when overexpressed in electroporated adult soleus muscles.

10 In REMI, a restriction enzyme is electroporated along with linearized vector into cells,

11 efficiently captured, in the current work we electroporated an I-SceI expression plasmid along with H

12 , where small sample volumes are top-loaded, electroporated and bottom-ejected into 96-well plates.

13 Within 3 h, about 12 embryos can be electroporated and set up for culture by the New techniq

14 Comparison was made in untreated, electroporated, and SL-O permeabilized KYO1 cells.

15 Newly fertilized dwarf surfclam eggs were electroporated at 700 V in the presence of 1 x 10(4) col

16 ce to Ksm and Spc was never observed in mock electroporated bacteria (frequency <6.2 x 10(-9)).

17 The percentages of electroporated bacteria were found to be more than an or

18 ing indicates that in osmotically shocked or electroporated bacteria, proteins are strained through a

19 HL60 cells did not electroporate, but CEM cells were efficiently electropor

20 his study develops a model for a single cell electroporated by an external electric field and uses it

21 previously developed model of a single cell electroporated by an external electric field by explicit

22 Vero cells were infected with electroporated C. burnetii and transformants scored as o

23 transiently active anti-CD123 messenger RNA-electroporated CART (RNA-CART123); (2) T-cell ablation w

24 state and the mass transfer rate through the electroporated cell membrane.

25 ylene oxide)-poly(ethylene oxide) helps seal electroporated cell membranes, arresting the leakage of

26 ranging from less than 10(-7) to 10(-6) per electroporated cell.

27 molecules, as well as manipulate, sort, and electroporate cells in a programmable manner.

28 sp2 and SARSDeltansp2 viruses recovered from electroporated cells had 0.5 to 1 log10 reductions in pe

29 Luciferase activity in electroporated cells peaked on day 2 at levels 6 orders

30 Profiling RNAs from electroporated cells showed a defect in endogenous let7

31 ion of the receptor promote migration of the electroporated cells to the cerebral cortex.

32 We achieved 81 +/- 1.7% (N = 6) electroporated cells with 17 +/- 8% (N = 5) cell death u

33 SL-O permeabilized cells when compared with electroporated cells, suggesting that, after SL-O permea

34 In SL-O permeabilized or electroporated cells, uptake was approximately 1 to 2 lo

35 We show here that electroporated channelrhodopsin-2 can be activated in ov

36 nd therapy response as vaccination with mRNA-electroporated DCs.

37 By electroporating DRGs with small interfering RNAs against

38 We electroporated dye into cells in different frequency reg

39 te here, the same proteins are released from electroporated E. coli cells pretreated with EDTA.

40 ion of mutagenized Ci-Bra/lacZ transgenes in electroporated embryos.

41 om an otherwise normal lacZ reporter gene in electroporated embryos.

42 d with known experimental values required to electroporate Escherichia coli.

43 ile viral RNA transcripts were produced from electroporated expression vectors, as determined by reve

44 ase studies of exocytosis, including a large electroporated granule of the mast cell, a small and cle

45 erase (CAT) or luciferase, was detected when electroporating in the presence of 50 microg closed-circ

46 ed animal growth occurred following a single electroporated injection of a mixture of two plasmids (1

47 ere cloned into the shuttle vector pLS88 and electroporated into 4074Deltacps1N to produce 4074Deltac

48 This construct was electroporated into a B. burgdorferi host strain carryin

49 e cloned STb gene (in the plasmid pRAS1) was electroporated into a nonenterotoxigenic strain (226M) w

50 enomes containing PdG on the (-)-strand were electroporated into a uvrA(-) strain.

51 Finally, an FGF8 producing plasmid was electroporated into an ectopic site in the ventral phary

52 The promoter constructs were electroporated into axolotl limb blastemas and the wild

53 The DNA is then purified, and subsequently electroporated into bacteria.

54 hmania RNA virus 1-4 (LRV1-4) sequences were electroporated into cells of Leishmania strain M4147, a

55 External Ca2+ electroporated into cells preloaded with the catalytic d

56 alysis, ligated into pre-prepared vector and electroporated into competent Escherichia coli cells.

57 he cpe open reading frame of NCTC 8239, were electroporated into cpe-negative C. perfringens type A,

58 from the in vitro reaction was recovered and electroporated into Escherichia coli.

59 f 222 resultant fusion genes were separately electroporated into fertilized eggs, and their regulator

60 3' fragments of GLV positive-strand RNA, was electroporated into GLV-infected trophozoites.

61 A plasmid containing this construction was electroporated into H. ducreyi 35000HP, and an isogenic

62 tance cassette (aphA) and this construct was electroporated into H. pylori ATCC 43504; allelic exchan

63 2 cDNA subcloned in a pIRES2-EGFP vector was electroporated into hMSCs.

64 cleic acids with very high affinity, were co-electroporated into immortal human cells along with a se

65 ed upstream of inhA in a plasmid vector, was electroporated into M. tuberculosis, and the resulting c

66 z2 promoter-driven luciferase reporter genes electroporated into maize protoplasts together with R an

67 This plasmid library was electroporated into Mycobacterium smegmatis 1-2c, and th

68 rental and xrn1delta mutant yeast strains or electroporated into plant protoplasts.

69 coding region were synthesized in vitro and electroporated into protoplasts that were cultured at 40

70 MB102 DNA, and the adducted viral genome was electroporated into repair-proficient and repair-deficie

71 These constructs were electroporated into rice protoplasts and transient expre

72 nd RING-H2 domain truncated rapsyn-EGFP were electroporated into sternomastoid muscles, a strong raps

73 The plasmid was electroporated into T. denticola, and double-crossover r

74 e P210bcrabl32D cell line, we transiently co-electroporated into the growth factor dependent 32D cell

75 otactic in vivo, dominant negative FGFR1 was electroporated into the premigratory cardiac neural cres

76 isolated from these clones, and TCR RNA was electroporated into the same non-MART-1-reactive allogen

77 ology to the integrated 3' portion, was then electroporated into the target cell lines, and selection

78 M13MB102 DNA, and the adducted genomes were electroporated into wild-type or mutS-deficient Escheric

79 These data support the use of mRNA-electroporated LCs, or moDCs supplemented with exogenous

80 on was significantly impaired in Dok-7-siRNA-electroporated legs compared with the contralateral cont

81 lies functions that protect and recombine an electroporated linear DNA substrate in the bacterial cel

82 ome engineering in Escherichia coli by using electroporated linear DNA.

83 ort, we have developed a simple technique to electroporate mammalian cells with high throughput on a

84 selectively adsorbs into damaged portions of electroporated membranes, thereby localizing its effect.

85 nsport of various fluorescent markers across electroporated membranes.

86 Furthermore, we also explored ex vivo electroporated mouse erythrocyte as the carrier of RNA.

87 expression of 5HT6 and SSTR3 was achieved by electroporating mouse embryo cortex in utero with vector

88 hesis and direct measurement of radiolabeled electroporated mRNA indicate that the primary effect of

89 junction (approximately 25%) in Dok-7-siRNA-electroporated muscles, compared with contralateral cont

90 oth spiny and aspiny neurons surrounding the electroporated neuron in each case, in addition to intri

91 th transsynaptic labeling occurring in every electroporated neuron infected by the virus.

92 The ski6-2 mutant expressed electroporated non-poly(A) luciferase mRNAs 8- to 10-fol

93 The slh1Delta ski2Delta strains translate electroporated nonpoly(A) mRNA with the same kinetics as

94 of GABA(A)-induced calcium responses in most electroporated NPCs led to a 70% decrease in the number

95 nduce formation of pancreatic tumor foci, we electroporated oncogenic plasmids into pancreata of LSL-

96 Using a novel system for microinjecting and electroporating plasmid expression constructs into murin

97 To examine GABA(A) function in vivo, we electroporated plasmids encoding short-hairpin (sh) RNA

98 nditional regulation of gene expression from electroporated plasmids in the postnatal rat retina and

99 In this study, we show that RNA electroporated post maturation and coelectroporated with

100 nts and in transient expression assays using electroporated protoplasts of tobacco.

101 tripped porcine stratum corneum following an electroporating pulse were studied by time-resolved free

102 By live imaging of in utero electroporated rat brain, we find that two distinct G2-s

103 recombinant Mycobacterium bovis BCG (rBCG), electroporated recombinant DNA (rDNA) along with an inte

104 We successfully electroporated recombinant mouse mitochondrial genomes i

105 t the largest pores are on the border of the electroporated regions of the cell.

106 Experienced users can electroporate roughly 40 tadpoles per hour.

107 lular ionic concentrations induced around an electroporated single cell.

108 We show examples of successfully electroporated single cells, instances of common problem

109 application of short, high-voltage pulses to electroporate skin has recently been shown to make it re

110 requent genetic alterations of human ICC, we electroporated Sleeping Beauty-based oncogenic transposo

111 CAR expression and function of RNA-electroporated T cells could be detected up to a week af

112 Multiple injections of RNA CAR-electroporated T cells mediated regression of large vasc

113 r cell line, large cells should be easier to electroporate than small ones: the minimum electric fiel

114 mV) rectangular voltage pulses were used to electroporate the bilayers, followed by a prolonged, low

115 t the end of the first recording session, we electroporated the neuron with a DNA plasmid to drive GF

116 To investigate this question, we electroporated the OV with anti-Pax6 or control morpholi

117 sulates cells into aqueous droplets and then electroporates the encapsulated cells.

118 (17328) or PS/PD/ME- (17359) IL-2 oligo was electroporated to mouse T cell lymphoma cells (TIB 155)

119 Cells electroporated with 20 microg of the NEO construct were

120 OHCs were consistently electroporated with 50 ms pulses, with significant resis

121 To introduce a genomic DSB, cells were electroporated with a plasmid expressing endonuclease I-

122 Cells were then electroporated with a plasmid expressing endonuclease I-

123 Neurons electroporated with a shRNA targeting IGF-1 receptor fai

124 n response to araC, initiated apoptosis when electroporated with active PP1alpha.

125 18 +/- 81 RGCs/mm(2) were present in retinas electroporated with alphaA and alphaB expression constru

126 ctivity was significantly inhibited in VSMCs electroporated with anti-Gbeta antibody (56 +/- 5%) and

127 Furthermore, in VSMCs electroporated with anti-Gbeta antibody and cells stably

128 tiple injections of autologous human T cells electroporated with anti-mesothelin CAR mRNA.

129 and protoplasts from each tobacco line were electroporated with both DNA encoding the nuclease and d

130 sors of these cortical neurons were in utero electroporated with CBSH3- or CBSH3+ DNAs, migrated to t

131 Surprisingly, embryos electroporated with dsRNA targeting PTP-delta together w

132 organisms, embryonic chick spinal cords were electroporated with either construct, allowing the frequ

133 Analysis of mouse embryos electroporated with FMRP small hairpin RNA and knock-out

134 Rat soleus muscles were electroporated with green fluorescent protein (GFP) repo

135 Undifferentiated ES cells were electroporated with HCActlacZ together with a plasmid co

136 logous dendritic cells infected with VACV or electroporated with individual VACV protein mRNAs.

137 H9A cells were electroporated with inducible expression vectors (under

138 n resistance marker, and this strain is then electroporated with suicide plasmids containing mariner

139 ogous monocyte-derived dendritic cells (DCs) electroporated with synthetic mRNA (TriMixDC-MEL) are im

140 Animals electroporated with the beta1 subunit plasmid showed a t

141 DSBs occurring in as many as 1 of 100 cells electroporated with the endonuclease expression vector.

142 rRNA gene were observed when C. psittaci was electroporated with the recombination substrates.

143 Finally, cells electroporated with the shRNA targeting IGF-1 receptor w

144 iously transfected with the SV40 vector were electroporated with the TD-TFOs, frequencies of base pai

145 C. psittaci 6BC infectious particles were electroporated with various concentrations of circular o

146 Control embryos electroporated with vehicle, or with dsRNA targeting cho

147 ors exiting the cell cycle compared to cells electroporated with wild-type CCND2.

148 LCs electroporated with Wilms tumor 1 (WT1) mRNA achieve suf