ライフサイエンスコーパス: electroporation

1 mosaic mice were generated through in utero electroporation.

2 lis anterior muscle of adult rats by in vivo electroporation.

3 ld threshold required for inducing bacterial electroporation.

4 nt cells utilizing mechanical penetration or electroporation.

5 the increase in fluorescence intensity after electroporation.

6 n mammalian cells that had been subjected to electroporation.

7 as delivery by needle injection followed by electroporation.

8 erve and in spinal nerve axons after in vivo electroporation.

9 siRNA was then encapsulated into the EMs by electroporation.

10 ic delivery, agitation with glass beads, and electroporation.

11 ents including in vivo electrophysiology and electroporation.

12 f the embryonic rat neocortex using in utero electroporation.

13 n tumor models and normal tissues to calcium electroporation.

14 eneral route to enhance the functionality of electroporation.

15 ector or caCaMKKalpha plasmids using in vivo electroporation.

16 by specific Ebp1 down-regulation using shRNA electroporation.

17 .m. immunization in combination with in vivo electroporation.

18 ely 85%) as well as rapid pore closure after electroporation.

19 that minimizes exosome aggregation following electroporation.

20 uperior immunogenicity than delivery without electroporation.

21 ores into live Escherichia coli by employing electroporation.

22 rve can be accomplished by 12-ns PEF without electroporation.

23 healthy volunteers who received ADVAX DNA by electroporation.

24 ateral potential field and thus facilitating electroporation.

25 irus-like replicon particles (VRP) or direct electroporation.

26 mperature-dependent process that facilitates electroporation.

27 ric field to magnitudes sufficient to induce electroporation.

28 nificantly smaller threshold field to induce electroporation.

29 viding neuronal progenitors through in utero electroporation.

30 ing based on nonthermal partial irreversible electroporation.

31 Epicardial electroporation ablation after subxiphoid pericardial pu

32 ration, but the long-term response of PVs to electroporation ablation is unknown.

33 With electroporation ablation, PV ostial diameter decreased 1

34 of genomic B108 by CRISPR/Cas9 in vivo using electroporation abolished the function of Blimp1.

35 Use of electroporation after PV administration provided superio

36 the other hand, the electric field used for electroporation aggravates the toxicity of hydroxyl beca

37 hout the need for transfection agents and/or electroporation, allowing cell-tracking by MRI in both p

38 in layer 2/3 pyramidal neurons via in utero electroporation also reduced spine density in vivo.

39 ) into L2/3 pyramidal neurons using in utero electroporation also results in a hyperexcitable phenoty

40 endogenous environment, using mouse in utero electroporation and a conditional genetic strategy to mi

41 ectric fields was separated into a transient electroporation and a persistent permeabilization.

42 Using plasmid electroporation and AAV viral vectors, we delivered CRE-

43 ed delivery of zinc finger nuclease mRNA via electroporation and adeno-associated virus (AAV) 6 deliv

44 ble of continuous, as opposed to batch-wise, electroporation and cell analysis.

45 improvement in colony formation relative to electroporation and cell-penetrating peptides.

46 or digitorum brevis (FDB) fibers via in vivo electroporation and examined the abilities of these two

47 ls, mammalian antibiotic selection, a second electroporation and gene network activation.

48 es, into the mouse retina by ex vivo plasmid electroporation and into the mouse cerebral cortex by in

49 (60-75 kg), the response of PVs to circular electroporation and radiofrequency ablation was compared

50 intramuscularly in combination with in vivo electroporation and subsequently 5 or 12 weeks later boo

51 These vesicles rupture and open up after electroporation and the meshwork is expelled through a m

52 orods (AuNRs) were first loaded into PLTs by electroporation and the resulting AuNR-loaded PLTs (PLT-

53 nses, we enhanced cellular transfection with electroporation and then boosted the DNA-primed response

54 siently express HBV-specific TCRs using mRNA electroporation and to assess their antiviral and pathog

55 echnique called iTRAP that combined in utero electroporation and translating ribosome affinity purifi

56 rio requires the loss of membrane integrity (electroporation) and resulting depolarization as an inte

57 inations were delivered biweekly via in vivo electroporation, and both humoral and CD8 T cell respons

58 munized intramuscularly, followed by in vivo electroporation, and in these animal models, vaccination

59 f-function studies in the chick using in ovo electroporation, and loss-of-function studies in Arx-def

60 ffected cells by reversible and irreversible electroporation, and quantified the uptaken amount of na

61 prefrontal cortex of neonatal mouse pups by electroporation, and the regulation of MMP-9 transcripti

62 to tissue ablation: microwave, irreversible electroporation, and water vapor.

63 An electroporation apparatus hyphenated with stopped-flow s

64 orcine model, multiple circumferential 200-J electroporation applications inside the PV ostia do not

65 Ten consecutive, nonarcing, electroporation applications of 200 J were delivered 5 t

66 onbarotraumatic, cathodal 50, 100, and 200 J electroporation applications were delivered randomly on

67 Moreover, an in utero electroporation approach showed that PNH-related mutants

68 viability compared to the conventional bulk electroporation approach.

69 We evaluated three electroporation approaches: (1) a square-wave electropor

70 As MAE works like many single cell electroporation are carried out in parallel, the electro

71 s success might promote the wide adoption of electroporation as a safe and effective non-viral gene d

72 NA delivered intramuscularly by Biojector or electroporation at baseline and week 4 followed by intra

73 We show evidence that electroporation-based delivery does not involve endocyto

74 lectroporation (MAE) platform to advance the electroporation-based delivery of DNA and RNA probes int

75 Here, we describe a simple and economic electroporation-based strategy to deliver Cas9/sgRNA rib

76 Electroporation-based time-lapse experiments, exclusivel

77 Electroporation-based transfection methods have allowed

78 change of pH by modifying the composition of electroporation buffer.

79 -fold in tumor and skin tissue after calcium electroporation but decreased in skin tissue 4 hours aft

80 ve of the hydrophilic toroidal pore model of electroporation, but also reveal additional complexity i

81 ternative method of ablation is irreversible electroporation, but the long-term response of PVs to el

82 Electroporation by nanosecond electric pulses (nsEP) is

83 Overall, our results suggest that calcium electroporation can elicit a rapid and selective necrosi

84 Here, we demonstrate that on-line electroporation can serve as a method for membrane perme

85 We investigated lesion size after epicardial electroporation catheter ablation with various energy le

86 Cas9 RNP electroporation caused up to approximately 40% of cells

87 In normal muscle, SIRT1 overexpression by electroporation causes rapid fiber hypertrophy without,

88 s by purified transposase can be achieved by electroporation, chemical transfection or Lipofection of

89 ic postnatal knockdown of DISC1 via in utero electroporation combined with an inducible knockdown exp

90 it could be established experimentally that electroporation consists of two clearly separate process

91 lable approach to microfluidic, flow-through electroporation could facilitate the integration of elec

92 live cells by labeling native kinesins using electroporation-delivered QDs.

93 In utero, electroporation demonstrates that activation of the Nrp2

94 perior to that observed in the trial without electroporation, despite fewer vaccinations.

95 s vaccinated with PV plus IL-12 plasmid with electroporation developed either a CD4(+) or CD8(+) T-ce

96 e developed a novel nanofiber-based sandwich electroporation device capable of in situ and in culture

97 Here we present a flow-through cell electroporation device integrating large-sized flow tube

98 We present a microfluidic electroporation device with a comb electrode layout fabr

99 ee injection system (Biojector) or CELLECTRA electroporation device.

100 nts required to porate the membrane, current electroporation devices deliver voltage pulses in the kV

101 igh voltage, while the emerging microfluidic electroporation devices is still limited by their low ce

102 AdC7), Vaccinia virus (VV), and DNA given by electroporation (DNA/EP), all expressing Simian immunode

103 At 19 d post-electroporation (dpe), heterotopia and ectopic cells wit

104 Using in utero electroporation during corticogenesis, we show that incr

105 gap, we used calcium imaging and single-cell electroporation during oculomotor behaviors to map VPNI

106 In all tumor types tested in vivo, calcium electroporation effectively induced necrosis, with a ran

107 The present study compared electroporation efficiency of bipolar and unipolar nanos

108 nificant relationship between the amounts of electroporation energy delivered epicardially and lesion

109 e response to IM injection administered with electroporation (EP).

110 ansmembrane-anchored, cleaved JRFL Env or by electroporation (EP).

111 expression and were delivered using in vivo electroporation (EP).

112 In utero electroporation experiments showed that ectopic BEND6 in

113 s an electric pulse-generating nano-probe in electroporation experiments with a potential application

114 Determining the critical electric field for electroporation facilitates the development of electropo

115 of MyD88 in the muscle of wbMyD88KO mice via electroporation fails to restore atrophy gene induction

116 ors were delivered into the chondrocytes via electroporation followed by poly (beta-amino esters) (PB

117 ss that involves transformation of ssDNA (by electroporation) followed by outgrowth, during which bac

118 TPM maximized exosome dispersal post electroporation for both homogenous B16 melanoma and het

119 ed AGT monoepoxides into mammalian cells via electroporation generated AGT-DNA cross-links and induce

120 n array of four electrodes (18 gauge) and an electroporation generator.

121 h more immediate, but transient, pain in the electroporation group.

122 Administration of PV plus IL-12 with electroporation had a significant dose-sparing effect an

123 Although the contributing role of water in electroporation has been noted previously, here we propo

124 Electroporation has been widely used in delivering forei

125 Electroporation has previously been used to load exosome

126 echniques such as artificial endocytosis and electroporation have also been developed to achieve payl

127 Recently, methods such as in vivo electroporation have demonstrated improved performance,

128 s into exosome colloidal stability following electroporation have not been considered.

129 c protocol screening process in current bulk electroporation (i.e., electroporation to a large popula

130 In this study, we demonstrate that electroporation (i.e., the application of short electric

131 ne permeability increase usually ascribed to electroporation, i.e., formation of aqueous membrane por

132 intramuscular vaccinations were followed by electroporation in HVTN 080.

133 es against a model HIV antigen comparable to electroporation in mice, enhanced memory T-cell generati

134 Using shRNA in utero electroporation in mice, we show that Vrk1 knockdown sig

135 Maintenance of high SIRT1 levels by electroporation in mouse muscle inhibits markedly the mu

136 Using single cell electroporation in the neurogenic subventricular zone (S

137 Twenty-one dogs underwent injection+electroporation in the posterior left atrium of plasmid

138 PRG-2 electroporation in the PRG-2(-/-) thalamus restored the

139 osome aggregation and electroextraction post electroporation in TPM compared to common PBS pulse medi

140 te and conducting gene transfection via bulk electroporation (in suspension), which is then followed

141 ns; microwave ablation, in six; irreversible electroporation, in five; and cryoablation, in one.

142 xpression in the developing retina by in ovo electroporation increases the number of RGCs, whereas th

143 were designed to unambiguously separate the electroporation-induced sensitization and desensitizatio

144 y elevated Notch signaling, induced by N1ICD electroporation, inhibited gliogenesis in wildtype anima

145 Electroporation into strain RN4220 followed by temperatu

146 At 2 days after in utero electroporation into the ventricle of the developing bra

147 tion in bone marrow cells after irreversible electroporation (IRE) and to evaluate the antitumoral ef

148 Irreversible electroporation (IRE) as a non-thermal tumor ablation te

149 Irreversible electroporation (IRE) as newer ablation modality has bee

150 gate the safety of percutaneous irreversible electroporation (IRE) for locally advanced pancreatic ca

151 d electroporative ablation with irreversible electroporation (IRE) in appropriately selected animal m

152 gate the efficacy and safety of irreversible electroporation (IRE) in the treatment of hepatic tumors

153 sess the safety and efficacy of irreversible electroporation (IRE) in the treatment of patients with

154 Irreversible electroporation (IRE) is a promising non-thermal treatme

155 Irreversible electroporation (IRE) is a promising nonthermal ablation

156 Irreversible electroporation (IRE) is an emerging focal therapy which

157 Irreversible electroporation (IRE) is an energy delivery system, effe

158 ose To determine the effects of irreversible electroporation (IRE) on the neural tissues after ablati

159 adiofrequency (RF) ablation and irreversible electroporation (IRE), Bulvik et al demonstrated substan

160 lls treated with high-frequency irreversible electroporation (IRE).

161 Electroporation is a biophysical phenomenon that has sho

162 Irreversible electroporation is a novel, nonthermal ablation modality

163 Irreversible electroporation is a promising nonthermal ablation modal

164 Electroporation is a widely used technique to permeabili

165 Continuous cell electroporation is an appealing non-viral approach for g

166 Electroporation is commonly used to deliver molecules su

167 The potential application of CSMEN in electroporation is confirmed by analyzing crystallograph

168 In this way, cell size specific electroporation is conveniently carried out, contributin

169 tical step for initiating vesicle opening by electroporation is diffusion of membrane proteins away f

170 The efficacy of electroporation is known to vary significantly across a

171 Electroporation is the formation of permeabilizing struc

172 These data support the hypothesis that electroporation is the primary force for pore opening in

173 While electroporation is used extensively in biology, biotechn

174 Electroporation is used to create pores within the membr

175 adult rat cerebral cortex following in utero electroporation (IUEP) at embryonic stage E14.

176 enpj during cortical development by in utero electroporation knockdown and found that silencing Cenpj

177 n of Fat1 in cortical precursors by in utero electroporation leads to overproliferation of radial gli

178 ells, delivery of the BH4-Bcl-XL peptide via electroporation limits agonist-induced mitochondrial Ca(

179 Many techniques such as electroporation, lipofection or microinjection have been

180 Here we developed a new micropillar array electroporation (MAE) platform to advance the electropor

181 Calcium electroporation may offer a simple general tool for anti

182 We envision that electroporation may serve as a simple and universal tool

183 body-based prophylaxis/therapy entailing the electroporation-mediated delivery of synthetic DNA plasm

184 We used in utero electroporation-mediated EphA7 overexpression in develop

185 e mapping of the scaling relationships among electroporation-mediated molecular delivery, cellular vi

186 lectroporation phenomenon, or magneto-elasto-electroporation (MEEP), is discovered while studying the

187 The development of an electroporation method that permits rapid expression of

188 within the intact mouse oviduct by a simple electroporation method, and result in the desired geneti

189 y in reporter assay, compared to traditional electroporation method.

190 f cytoreagents.Current widely used viral and electroporation methods for creating therapeutic cell-ba

191 However, the viral and electroporation methods used to create cytoreagents are

192 Compared to lipofection and electroporation methods, plasma transfection showed a be

193 uring scaling down the size of electrodes in electroporation microchips.

194 poration could facilitate the integration of electroporation modules within cell analysis devices tha

195 pplied a unique delivery method, nanochannel electroporation (NEP), to produce predominantly nonendoc

196 e-mediated recombination as well as in utero electroporation of a Cre-expression construct identified

197 propose a novel molecular mechanism for the electroporation of a lipid bilayer based on energetics a

198 Electroporation of a plasmid encoding constitutively act

199 lencing in mouse embryonic brain by in utero electroporation of a specific Lmnb1 sh-RNA results in ab

200 Finally, in ovo electroporation of axonally targeted GAP-43 mRNA increas

201 Here, using electroporation of Cas9 nuclease/single-guide RNA ribonu

202 We used in utero electroporation of DsRedExpress- and eGFP-tagged postsyn

203 ence-based methods to demonstrate successful electroporation of E. coli.

204 Electroporation of either RORbeta isoform into retinal e

205 Electroporation of expression constructs encoding PMCA4a

206 Finally, targeted electroporation of Fgfr3 siRNA to prechordal mesoderm in

207 ular zone of the embryonic brain by in utero electroporation of Fut10-miRNAs impaired the radial migr

208 For the first 3 d after electroporation of HCV RNA, intracellular virus predomin

209 Electroporation of Hif1a targeting short hairpin RNA (sh

210 -clamp recording experiments and single-cell electroporation of identified rat and mouse neurons in v

211 Moreover, in vivo electroporation of IL-18 into skeletal muscle activated

212 In utero electroporation of L1-80 into reeler embryos normalised

213 Using in utero electroporation of mouse neocortices as well as zebrafis

214 riants was assessed by luciferase assays and electroporation of mouse retinal explants with reporter

215 Conversely, overexpression of miR-29 by electroporation of mouse tibialis anterior muscle decrea

216 In utero electroporation of mutant CCND2 into embryonic mouse bra

217 Here using in utero electroporation of NDE1 short hairpin RNA (shRNA) in emb

218 Electroporation of Nfia promoted the formation of cells

219 After electroporation of oncogenic plasmids, mice developed a

220 In vivo postnatal electroporation of phosphomimetic (S39D) or nonphosphory

221 Furthermore, in vivo electroporation of PRCD C2Y mutant in the mouse retina d

222 primary equine fetal liver cultures or after electroporation of selectable replicons.

223 Silencing of Bclaf1expression by in vitro electroporation of shRNA in embryonic retina confirmed t

224 Here we report that in utero electroporation of shRNA, but not siRNA or miRNA, to Dcx

225 AICD delivery or APP knock-down by in utero electroporation of shRNAs with whole-cell electrophysiol

226 nt knockdown of USP14 or UCHL5 expression by electroporation of siRNA reduced the viability of multip

227 strate that simple intramuscular delivery by electroporation of synthetic DNA plasmids engineered to

228 rs to find the optimal set of conditions for electroporation of target species.

229 Here we show that electroporation of transcription activator-like effector

230 the glucocorticoid receptor (GR) gene using electroporation of transcription activator-like effector

231 Here we report that combining electroporation of zinc finger nuclease (ZFN) mRNA with

232 Our methodology, designated as CRISPR RNP Electroporation of Zygotes (CRISPR-EZ), enables highly e

233 y varying the parameters associated with the electroporation operation.

234 eleventh strand of the GFP protein either by electroporation or by cell-penetrating peptide-mediated

235 Since it does not require electroporation or microinjection, this tool has the pot

236 hed transposon-based protocols which require electroporation or microinjection.

237 , the system enables on-chip optimization of electroporation parameters for cells with varying proper

238 The optimized electroporation parameters reported here provide a usefu

239 For traditional electroporation parameters, larger cells experience a gr

240 The cell electroporation phenomenon and its correlation with the

241 A magnetically controlled elastically driven electroporation phenomenon, or magneto-elasto-electropor

242 ection procedure was developed that involves electroporation, plasmids replication in mammalian cells

243 CAPZB2 expressed by in utero electroporation prevented normal elongation of vestibula

244 In conclusion, DNA vaccination by electroporation primed for TCR clonotypes that were asso

245 ly relevant environment for the study of the electroporation process.

246 ectroporation facilitates the development of electroporation protocols that minimize Joule heating an

247 nd frequency-dependent effects in developing electroporation protocols, and our approach provides, to

248 eadily take up pyruvate, the addition of the electroporation pulse to the D-DNP experiment increases

249 ion threshold than conventional irreversible electroporation pulse trains, at the expense of larger a

250 ates the cell death response to conventional electroporation pulsed-electric fields ( approximately 1

251 mains unaffected 2 months after irreversible electroporation, purposely targeting the adventitia.

252 to the improved antigen delivery afforded by electroporation rather than modulation of immunodominanc

253 of cell membranes by pulsed electric fields (electroporation) remain obscure despite decades of inves

254 etween cell morphology, pulse frequency, and electroporation response.

255 Vs using various passive and active methods (electroporation, saponin, extrusion and dialysis).

256 ates blind (non-visually guided) single-cell electroporation (SCE) and extracellular electrophysiolog

257 Irreversible electroporation seems to be a safe modality for catheter

258 Electroporation serves as a promising non-viral gene del

259 This new view of electroporation simplifies the study of the problem, and

260 We therefore leveraged the in vivo electroporation strategy used in utero in rodents and in

261 sibility of the vortex-assisted microfluidic electroporation system for direct drug cocktail analyses

262 eveloped a versatile on-chip vortex-assisted electroporation system, engineered to conduct sequential

263 ation in mouse corticogenesis using in utero electroporation targeted to projection neurons.

264 Protein (GFP), together with transgenic and electroporation technologies, have made it possible to e

265 eported a delayed increase of sensitivity to electroporation (termed "electrosensitization") in mamma

266 ons to the Smoluchowski-Einstein equation of electroporation that is dependent on the pore conductanc

267 cesses: a rapid membrane poration (transient electroporation) that occurs while the membrane is depol

268 After electroporation, the transposon/transposase improves the

269 erties but to date has not been addressed by electroporation theory or MD simulations.

270 The use of electroporation therefore extends the applicability of D

271 e TMP of tightly packed cells to a simulated electroporation threshold than conventional irreversible

272 Reversible electroporation thresholds were 54% lower than LTs for s

273 ocess in current bulk electroporation (i.e., electroporation to a large population of cells).

274 We use electroporation to deliver CRISPR/Cas9 components for ti

275 e data support the feasibility of using mRNA electroporation to engineer HBV TCR-redirected T cells i

276 ion that combines tissue microinjection with electroporation to express cDNAs and shRNAs in mouse coc

277 Using in utero electroporation to manipulate the sumoylation state of F

278 nsfected into fetal mouse brains by in utero electroporation to test the effects of E6 on cortical de

279 parameters inspired by clinical irreversible electroporation treatments.

280 We found that partial irreversible electroporation using 200 pulses of 250 V and 70 mus dur

281 rotein-mediated transduction and single-cell electroporation via the EnvA-TVA or EnvB-TVB (envelope g

282 fine balance between high flow rate and low electroporation voltage were steered clear.

283 Electroporation was achieved by bursts of 300-ns, 9 kV/c

284 administered intramuscularly and followed by electroporation was assessed in mice.

285 f TPM to disaggregate melanoma exosomes post electroporation was dependent on both exosome concentrat

286 s, immunofluorescence analysis, and in utero electroporation, we find that JIP3 can enhance axon elon

287 By in utero electroporation, we found that Oc1 and Ptf1a together ar

288 Using in utero electroporation, we here report that MBG3 mouse models c

289 Using in utero electroporation, we show here that the IGF-1R is essenti

290 Furthermore, through in utero electroporation, we show that downregulation of TBC1D23

291 bution and the toxicity of pH changes during electroporation, which significantly decreases cell viab

292 Co-electroporation with a constitutively active form of PI3

293 ciparum thereby serving as an alternative to electroporation with an increase in transfection efficie

294 model consisted of U2OS cells transfected by electroporation with HPV18 minicircles.

295 We compared DCs activated by electroporation with mRNA encoding constitutively active

296 rate that conditioning of mammalian cells by electroporation with nanosecond pulsed electric field (n

297 e cortical upper layers was also affected by electroporation with shRNA targeting IGF-1 receptor.

298 gold-microtube membranes that can accomplish electroporation with voltage pulses that are orders of m

299 and HPV-18 E6 and E7 proteins, delivered by electroporation, would cause histopathological regressio

300 efficacy was optimized by i.m. delivery via electroporation, yet it remained modest compared with Ad