ライフサイエンスコーパス: embryoid body

1 ive splice variant CoAM in the cavity of the embryoid body.

2 ed for protocols based on stromal feeders or embryoid bodies.

3 tion was substantially blunted in Gsk3b(-/-) embryoid bodies.

4 o S phase and mES cells differentiating into embryoid bodies.

5 to promote vascular development in Fgfr1-/- embryoid bodies.

6 ce positioning in Dab2-deficient embryos and embryoid bodies.

7 form a primitive endoderm outer layer in the embryoid bodies.

8 under hypoxia provided enhanced formation of embryoid bodies.

9 mechanism is also adopted in differentiated embryoid bodies.

10 icient at generating neurectoderm-containing embryoid bodies.

11 othelial cell precursors in developing mouse embryoid bodies.

12 LK1(+)CD4(-) cells first arise in developing embryoid bodies.

13 vivo and in embryonic stem (ES) cell-derived embryoid bodies.

14 arker, Hnf4, and prevents cavitation in PSA1 embryoid bodies.

15 tion of, visceral endoderm and cavitation of embryoid bodies.

16 ion factors in nuclear extracts from ES cell embryoid bodies.

17 ptional regulation in murine ES cell-derived embryoid bodies.

18 lian embryogenesis can be studied in ES cell embryoid bodies.

19 for the formation of a basement membrane in embryoid bodies.

20 formed a complex neurite network around the embryoid bodies.

21 xic Hif1a-/- embryonic stem cells and cystic embryoid bodies.

22 erved more often in wild type than in mutant embryoid bodies.

23 ificantly lower when compared with wild-type embryoid bodies.

24 omyocytes during in vitro differentiation of embryoid bodies.

25 as a floating scaffold to generate elongated embryoid bodies.

26 tion both in ES cells and in differentiating embryoid bodies.

27 ection neurons in monolayer culture and from embryoid bodies.

28 ronal differentiation and the utilization of embryoid bodies.

29 by loss-of-function experiments in chimeric embryoid bodies.

30 type and mutant ES cells and differentiating embryoid bodies.

31 ive vascular network from SIRT1(-/-)-derived embryoid bodies.

32 ), and differentiated cells via formation of embryoid bodies (16, 20 days) were analyzed.

33 PP2 during embryoid body adhesion dramatically increased cardiomyoc

34 ditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible dif

35 tes were seen in both wild-type and Gata4-/- embryoid bodies, although cardiomyocytes were observed m

36 lpha5-null ES cells were differentiated into embryoid bodies, although they were delayed in growth an

37 apoptosis-dependent process of cavitation in embryoid bodies and apoptosis associated with embryonic

38 tro differentiation potentials of iPSCs into embryoid bodies and different hematopoietic cell types.

39 Pten-/- ES cells formed aberrant embryoid bodies and displayed an altered ability to diff

40 how that Bmp2 and Bmp4 are expressed in PSA1 embryoid bodies and embryos at the stages when visceral

41 Upon differentiation into embryoid bodies and further into mineral-producing osteo

42 m cells relative to the core cells in mature embryoid bodies and in the visceral endoderm of Day 6.5

43 on of flk1(+) angioblasts in differentiating embryoid bodies and increased the number of PECAM (plate

44 is repressed as ES cells differentiate into embryoid bodies and is undetectable in adult mouse organ

45 rs while increasing the numbers of secondary embryoid bodies and mixed hematopoietic colonies obtaine

46 Although embryoid bodies and organoids can exhibit some spatial o

47 o the NA extract enhanced differentiation of embryoid bodies and resulted in the early appearance of

48 haracterized by vigorous beating activity of embryoid bodies and robust expression of cardiac Mef2c,

49 em cell markers; have the capability to form embryoid bodies and teratomas, and can differentiate int

50 ro, pig-a- ES cells were able to form pig-a- embryoid bodies and to undergo hematopoietic (erythroid

51 as the TaqMan hPSC Scorecard Assay) through embryoid body and directed differentiation experiments a

52 o iPSCs and differentiated into iPSC-CMs via embryoid body and monolayer-based differentiation protoc

53 during differentiation of ES cells in vitro (embryoid bodies) and in vivo (teratomas).

54 ere first expressed at day 4 in hanging drop embryoid bodies, and adhesion of embryoid bodies to surf

55 re differentiated into cardiomyocytes within embryoid bodies, and contracting cells expressing myocar

56 Here we isolate primordial germ cells from embryoid bodies, and derive continuously growing lines o

57 d spontaneous differentiation in cultures of embryoid bodies, and each of these steps involves signif

58 ized with flk1 expression in differentiating embryoid bodies, and HoxB5 potently transactivated the f

59 e hematopoietic stem cell differentiation in embryoid bodies, and large embryonic stem cell (ES)-deri

60 gocytosis were detected in PU.1(Spi-B/Spi-B) embryoid bodies, and myeloid colonies were present in he

61 progenitor cells present in differentiating embryoid bodies, and that these correspond to erythro-my

62 naling assay; 3) Pofut1(-/-) and Pofut1(+/+) embryoid bodies are indistinguishable in their ability t

63 cells before and during differentiation into embryoid bodies as well as various types of normal and t

64 he activation of developmental regulators in embryoid body assays.

65 For example, embryoid body-based analyses demonstrated that BAF250a-a

66 mesodermal lineages do not form in Wt1-null embryoid bodies, but this effect is rescued by the expre

67 mouse ES cells and that FAK signaling within embryoid bodies can direct stem cell lineage commitment.

68 activated cell sorting purification of human embryoid body cells differentially expressing endothelia

69 t the growth or viability of ES cell-derived embryoid body cells known to have extinguished TDH expre

70 Immunohistochemical analysis of embryoid bodies collected from these cultures revealed a

71 tiate in vitro into cystic structures called embryoid bodies consisting of tissue lineages typical of

72 Embryonic stem cell-derived embryoid bodies contain a unique precursor population wh

73 Nodal-expressing hESCs developing as embryoid bodies contained an outer layer of visceral end

74 inoic acid, into either parietal endoderm or embryoid bodies, containing an outer visceral endoderm l

75 on of primitive ectoderm and neurectoderm in embryoid body culture.

76 Depletion of Smad1 in embryoid body cultures before hemangioblast commitment l

77 We find that in embryoid body cultures containing even a low ratio of th

78 Using embryoid body cultures of mouse embryonic stem cells, we

79 e cell suspensions obtained from day 7 human embryoid bodies (d7EBs) injected i.v. 1 hour after cecal

80 A study using embryoid bodies demonstrated a nonimmediate role played

81 of yolk saclike hematopoietic development in embryoid bodies derived from CD34-null ES cells showed a

82 Embryoid bodies derived from embryonic stem cells recapi

83 s also different from the null phenotype, as embryoid bodies derived from ES cells in which endogenou

84 Differentiated embryoid bodies derived from GATA6(-/-) ES cells lack a

85 ind that hematopoietic CD34(+) cells in spin embryoid bodies derived from human embryonic stem cells

86 We demonstrate that embryoid bodies derived from KLF2(-)(/)(-) ES cells can

87 In this study, we used differentiating embryoid bodies derived from mouse embryonic stem cells

88 The embryoid bodies derived from mutant cells are also unabl

89 Embryoid bodies derived from these cell lines are unable

90 ls derived from embryonic germ cells, termed embryoid body-derived (EBD) cells, introduced into the C

91 undifferentiated embryonic stem (ES) cells, embryoid body-derived cells (EBCs), or mammalian embryos

92 ntification and characterization of an early embryoid body-derived colony, termed the transitional co

93 We show that embryoid body-derived hematopoietic progenitors expressi

94 In this study, the osteoinductivity of embryoid body-derived material (EBM) was compared to DBM

95 onal mutation, and has been termed EB-PE for embryoid body-derived primitive erythroid.

96 We sought to use human embryoid body-derived stem cells (EBDs) to populate live

97 in mouse embryonic stem cells and from human embryoid-body-derived cells, but not from human adult so

98 1 in ES cells and used ES/OP-9 coculture and embryoid body development followed by hematopoietic colo

99 at develops early and is lost quickly during embryoid body development.

100 and impaired primitive ectoderm formation in embryoid bodies differentiated from mouse embryonic stem

101 zyme activity occurring at later stages when embryoid bodies differentiated toward cardiomyocytes.

102 Upon aggregation to embryoid bodies, differentiating ES cells formed large n

103 In an embryoid body differentiation assay, BMP4-dependent diff

104 sion of the transcription factor Pax3 during embryoid body differentiation enhances both paraxial mes

105 nd endothelial progenitor cells by using the embryoid body differentiation method.

106 Using the embryoid body differentiation system, we demonstrate tha

107 ematopoietic lineage (from day 4 to day 6 of embryoid body differentiation) significantly enhances th

108 a) gene between 14 and 18 days of ES-derived embryoid body differentiation, we investigated the effec

109 illary-like structures during late stages of embryoid body differentiation.

110 Levels of Neu5Gc on HESC and embryoid bodies dropped after culture in heat-inactivate

111 s study, using ESC derived aggregates called embryoid bodies (EB) as a model, we characterized the bi

112 Here, a live stem cell derived embryoid body (EB) based cardiac cell syncytium served a

113 d development by means of coculture of CD34+ embryoid body (EB) cells with OP9 stromal cells.

114 ) hPGCLCs [ approximately 43% of FACS-sorted embryoid body (EB) cells] from primed-state induced plur

115 ental patterns of cellular expression during embryoid body (EB) differentiation can address this issu

116 jnk3 genes were derived and submitted to an embryoid body (EB) differentiation protocol.

117 The process of embryoid body (EB) differentiation, like teratoma format

118 of cells present at early and late stages of embryoid body (EB) differentiation.

119 We differentiated hESCs by embryoid body (EB) formation and compared the miR expres

120 Embryoid body (EB) formation is a requisite step in the

121 val, using annexin V staining, and secondary embryoid body (EB) formation were also evaluated.

122 to decreased pluripotency marker expression, embryoid body (EB) formation, cell survival, and loss of

123 l fate at the expense of the endoderm during embryoid body (EB) formation.

124 er capacity to self-renew based on secondary embryoid body (EB) formation.

125 gnificant haploinsufficient determinants for embryoid body (EB) formation.

126 aucity of paraxial mesoderm formation during embryoid body (EB) in vitro differentiation and to the l

127 S and ES cells were differentiated using the embryoid body (EB) method.

128 h the number and the size of beating foci in embryoid body (EB) outgrowths.

129 em (ES) cells undergo differentiation in the embryoid body (EB) system, with peak levels in cell popu

130 stablishment of the blood islands and in the embryoid body (EB)-derived blast-colony-forming cells (B

131 To improve the efficiency of embryoid body (EB)-mediated ES cell differentiation, we

132 e ES cells than in differentiating mouse ES (embryoid body, EB) cells.

133 yonic stem (ES) cells were differentiated as embryoid bodies (EBs) and assayed for blast colony-formi

134 bstrates generate size- and shape-controlled embryoid bodies (EBs) and can be easily modified to cont

135 mically defined medium supports formation of embryoid bodies (EBs) and differentiation of hepatic lin

136 We found that ERbeta was induced in embryoid bodies (EBs) and neural precursor cells (NPCs)

137 (VE-cadherin)-expressing cells generated in embryoid bodies (EBs) and on OP9 cells.

138 iation, as seen in poorly differentiated TKO embryoid bodies (EBs) and teratomas.

139 at hTERT silencing during differentiation to embryoid bodies (EBs) and to fibroblast-like cells was d

140 , we used embryonic stem cell-differentiated embryoid bodies (EBs) as a model and found that Bnip3 (B

141 dy we used mouse embryonic stem cell-derived embryoid bodies (EBs) as a model for peri-implantation d

142 S) cells can differentiate in vitro, forming embryoid bodies (EBs) composed of derivatives of all thr

143 Embryoid bodies (EBs) derived from cells lacking the aut

144 e stages of ExEn differentiation in cultured embryoid bodies (EBs) derived from either embryonic stem

145 Compared to wildtype (WT) controls, embryoid bodies (EBs) derived from either Lefty or Cerb-

146 Measuring the Shh response in neuralized embryoid bodies (EBs) derived from embryonic stem (ES) c

147 n, calponin, and LPP, were down-regulated in embryoid bodies (EBs) derived from embryonic stem cells

148 Here we demonstrate that embryoid bodies (EBs) differentiated from talin1-null em

149 cadherin positive cells developed within the embryoid bodies (EBs) formed by differentiating ES cells

150 ut embryonic stem cells (VIM -/- ESCs) using embryoid bodies (EBs) formed from both cell types.

151 Embryoid bodies (EBs) generated from embryonic stem cell

152 n, in the liver throughout fetal life and in embryoid bodies (EBs) generated from ES cells differenti

153 Wnt2(-/-) embryoid bodies (EBs) generated increased numbers of Flk

154 te into cell types of all germ layers within embryoid bodies (EBs) in a highly variable manner.

155 endoderm following their differentiation to embryoid bodies (EBs) in culture.

156 describe the internal organization of murine embryoid bodies (EBs) in terms of the structures and cel

157 tiating ES cells into cardiomyocyte-positive embryoid bodies (EBs) in vitro.

158 Rac1 ablation in embryonic stem cell-derived embryoid bodies (EBs) leads to massive apoptosis of epib

159 arge numbers of homogeneous and synchronized embryoid bodies (EBs) of defined sizes from dissociated

160 lls was dramatically delayed and impaired in embryoid bodies (EBs) of Shp-2 mutant origin.

161 c stem (ES) cells as they differentiate into embryoid bodies (EBs) or into extraembryonic endodermal

162 Here, we differentiated mouse iPSCs into embryoid bodies (EBs) or representative cell types spann

163 Expression of RUNX1a in embryoid bodies (EBs) promotes definitive hematopoiesis

164 h endogenous nuclear huntingtin in wild-type embryoid bodies (EBs) was associated with PRC2 subunits

165 oximately 65%) of cocultured ES cell-derived embryoid bodies (EBs) were enriched in cardiac myocytes

166 l patterning signals, murine ES cell-derived embryoid bodies (EBs) were grafted into avian hosts.

167 m and abnormal PPAR-gamma pathway in beating embryoid bodies (EBs) with defined media, we established

168 ES cells were allowed to differentiate into embryoid bodies (EBs), compared to the wild-type and het

169 peptide to the media enhanced the growth of embryoid bodies (EBs), increased the expression of hemat

170 Upon ES cell differentiation into embryoid bodies (EBs), we observed a shift in expression

171 In vitro, ES cells aggregate to form embryoid bodies (EBs), which differentiate into multiple

172 rmation of multicellular aggregates known as embryoid bodies (EBs), yet cell fate specification withi

173 affected by the size of ES cell colonies and embryoid bodies (EBs).

174 enerated in embryonic stem (ES) cell-derived embryoid bodies (EBs).

175 ions of hES-CMs in spontaneously contracting embryoid bodies (EBs).

176 arliest surface marker missing from SCL(-/-) embryoid bodies (EBs).

177 elopment in embryonic stem (ES) cell-derived embryoid bodies (EBs).

178 lished from in vitro-differentiated progeny (embryoid bodies [EBs]) of embryonic stem (ES) cells usin

179 layed a significant reduction in activity in embryoid bodies, embryos, and adult animals.

180 cultivation in vitro as 3D aggregates called embryoid bodies, ES cells can differentiate into derivat

181 Pharmacological responses of beating embryoid bodies exposed to a comprehensive panel of drug

182 Flk-1(+) Tet-notch4 cells from d 3 embryoid bodies exposed to doxycycline (Dox(+)) were com

183 with retinoic acid, the majority of cells in embryoid bodies expressed markers for neural progenitors

184 The ID6 line formed embryoid bodies, expressing genes representing all 3 ger

185 -1alpha/ARNT heterodimers) because Arnt(-/-) embryoid bodies fail to exhibit hypoxia-mediated progeni

186 different culture systems: FAK+/+ and FAK-/- embryoid bodies, FAK+/+ and FAK-/- endothelial cells, an

187 ion of a primitive streak-like population in embryoid bodies, followed by specification to hematopoie

188 g Ptp gamma antisense constructs and assayed embryoid bodies for the presence of hematopoietic precur

189 nd growth factors by ESCs differentiating as embryoid bodies for up to 14 days was assessed using PCR

190 Differentiating embryoid bodies form blood islands, providing an in vitr

191 ce markers, and differentiation potential in embryoid body formation and teratoma assays.

192 ed pluripotent stem cells (hiPSC), bypassing embryoid body formation and the use of exogenous molecul

193 of ectodermal and mesodermal lineages during embryoid body formation and under inductive conditions u

194 l direct plating method in which intervening embryoid body formation does not occur.

195 into multiple hematopoietic lineages during embryoid body formation in vitro, but to date, an ES-der

196 s required embryoid body formation; however, embryoid body formation often results in heterogeneous d

197 Data from embryoid body formation studies indicated that the Mp(-/

198 Embryoid body formation yielded beating cardiomyocyte-li

199 ed almost 30-fold during the first 3 days of embryoid body formation, a culture system model of early

200 ifferent in vitro differentiation protocols (embryoid body formation, endodermal induction, directed

201 for human embryonic stem (hES) cells rely on embryoid body formation, stromal feeder co-culture or se

202 ariants prevents the switch and disrupts the embryoid body formation.

203 ull differentiation capacity as indicated by embryoid body formation.

204 ted Nanog levels persisted through 5 days of embryoid body formation.

205 ifferentiation of ES cells in the absence of embryoid body formation.

206 ES cells overlapped with the changes during embryoid body formation.

207 ation from sorted single cells, and enhanced embryoid body formation.

208 ion of chondrogenesis by human ESCs required embryoid body formation; however, embryoid body formatio

209 roarrays to identify targets of Brachyury in embryoid bodies formed from differentiating mouse ES cel

210 lar criteria in the outer PE-like lineage of embryoid bodies formed from embryonic stem cell lines ge

211 Embryoid bodies, formed from mouse embryonic stem cells,

212 S) cells made extensive skeletal muscle, but embryoid bodies from myogenin (-/-) ES cells had greatly

213 oderm, which are prominent features of early embryoid bodies from normal ES cells.

214 Differentiated embryoid bodies from wild-type embryonic stem (ES) cells

215 ree media, human embryonic-stem-cell-derived embryoid bodies generate a KDR(low)/C-KIT(CD117)(neg) po

216 ultipotential precursor that develops within embryoid bodies generated from differentiated ES cells.

217 C development, spontaneously differentiating embryoid bodies give rise to CD105(+)CD90(+)CD73(+)CD31(

218 ) cell line H9, when cultured in the form of embryoid bodies, give rise to cells with markers of the

219 imitive endoderm cells of the outer layer of embryoid bodies gradually polarise, and formation of a p

220 irst appeared in embryonic stem cell-derived embryoid bodies grown for 7 days (7d).

221 Moreover, A(-)/A(-) embryoid bodies grown in suspension culture constantly s

222 nd definitive erythromyelopoiesis from human embryoid bodies (hEBs) in serum-free clonogenic assays.

223 The developmental sequence of human embryoid body hematopoiesis is remarkably congruent to t

224 ent development of keratinocytes from single embryoid bodies in cell culture.

225 re functionally abnormal; they yielded small embryoid bodies in in vitro differentiation experiments

226 The ES cell line D3 forms embryoid bodies in suspension culture without addition o

227 Cells cultured in HESCO readily form embryoid bodies in tissue culture and teratomas in mice.

228 AdiPS cells also generate embryoid bodies in vitro and teratomas in vivo.

229 cardiac differentiation was recapitulated in embryoid bodies in vitro.

230 n by HNF-3alpha and HNF-3beta was studied in embryoid bodies in which one or both HNF-3alpha or HNF-3

231 e was also reproduced in beta1 integrin-null embryoid bodies, in which primitive endoderm cells segre

232 at addition of BMP protein to cultures of S2 embryoid bodies induces expression of Hnf4 and other vis

233 alable two-dimensional method that avoids an embryoid-body intermediate.

234 levels of unbound histones, and formation of embryoid bodies is accelerated.

235 dent differentiation of endothelial cells in embryoid bodies is also antagonized by BMPER.

236 system, we found that induction of VEGFR1 in embryoid bodies is also associated with ETS1 and HIF-2al

237 cells into endothelial cells in an in vitro embryoid body is paralleled by an amplification of hepar

238 Studies of morphogenesis in embryoid bodies led to the current belief that it is pro

239 stem cells compromised their ability to form embryoid bodies, likely because of defects in cell proli

240 nd MyoD to support muscle differentiation in embryoid bodies made from myogenin (-/-) ES cells.

241 Like ES cells, embryoid bodies maintained constitutive Src and Fyn kina

242 Both HESC and derived embryoid bodies metabolically incorporate substantial am

243 human iPS clones were differentiated through embryoid body method and MYF5-GFP(+) myogenic cells were

244 Using a modified embryoid body method, we provided gene expression eviden

245 r cell, serum, conditioned culture medium or embryoid body, methods that cannot avoid undefined cultu

246 , we generated genetically mosaic neuralized embryoid bodies (nEBs) from mouse embryonic stem cells (

247 lated during development of Nodal-expressing embryoid bodies, nor was there induction of markers for

248 patic differentiation protocol starting from embryoid bodies of hiPSCs (hiPSC-EBs) for robust mass pr

249 up to 200 microm within 1 day of plating P19 embryoid bodies on laminin-1 (EHS laminin).

250 (LIF) and could initiate differentiation in embryoid bodies or chimeric embryos, but failed to commi

251 tides (DR1, DR2, DR5), we show that in mouse embryoid bodies or F9 embryonal carcinoma cells, RARs oc

252 Whether formed from embryoid bodies or in nodules, hES-derived keratinocytes

253 differentiated cells, methods that generate embryoid bodies or organoids do not yield consistent and

254 re, which does not require the generation of embryoid bodies or prospective cell isolation, entails f

255 eased on differentiation into differentiated embryoid body or neurospheres.

256 The inducible expression of EWS-FLI1 in embryoid bodies, or collections of differentiating stem

257 n vitro using the F9 teratocarcinoma derived embryoid body outgrowth system and, show here that PE mi

258 actor-beta treatment of isolated N629D/N629D embryoid bodies partially rescued this phenotype.

259 In particular, embryoid bodies produced from these Pgk-Pem ES cells do

260 4+ cells during ES cell differentiation from embryoid bodies provides an excellent model system and m

261 ction of mMix in embryonic stem cell-derived embryoid bodies results in the early activation of mesod

262 microscopy of cardiomyocytes in the Gata4-/- embryoid bodies revealed the presence of sarcomeres and

263 lly undefined factors including 3D nature of embryoid body, sera from animals, and the feeder cells i

264 ne embryonic stem cells converted to beating embryoid bodies showed that only the proximal active reg

265 mbryos and mouse embryonic stem cell-derived embryoid bodies substantially decrease the emergence of

266 ighted morphogenic cell processes within the embryoid bodies, such as cell growth, migration, and int

267 differentiation of mouse embryos and ES cell embryoid bodies suggest that aspects of early mammalian

268 these cells were induced to differentiate as embryoid bodies suggested that quite a few of the downre

269 We show that embryoid bodies support maturation of the primordial ger

270 Because embryoid bodies sustain blood development, we reasoned t

271 nalyses, we used an inducible embryonic stem/embryoid body system and observed that ER71 overexpressi

272 hat utilizes the differentiating ES cell and embryoid body system to define the modules and enhancers

273 Embryoid bodies that are cultured in the presence of nep

274 hibitory factor, mouse ES cells give rise to embryoid bodies that can differentiate into mesoderm.

275 e previously used two cell lines, which form embryoid bodies that do (PSA1) or do not (S2) cavitate,

276 When aggregated into embryoid bodies they develop disorganised masses of diff

277 that (a) laminin enables beta1-integrin-null embryoid bodies to assemble basement membrane and achiev

278 Finally, exposure of stem cell-derived human embryoid bodies to hsa-miR-1294 mimic or antagomir oligo

279 anging drop embryoid bodies, and adhesion of embryoid bodies to surfaces at or before that day strong

280 We exposed stem cell-derived human embryoid bodies to the microRNA mimic or antagomir oligo

281 IF-deficient cells and enabled AIF-deficient embryoid bodies to undergo cavitation, a process of prog

282 ricle with 40 to 75 rhythmically contracting embryoid bodies (totaling 1.3-2x10(6) cells).

283 ctopic beats were observed in 33% and 40% of embryoid bodies treated with sotalol and quinidine, resp

284 From day-6 embryoid bodies, under the influence of Stat5 signaling,

285 Differentiation of ES cells to embryoid bodies was associated with rapid transcriptiona

286 a dominant negative FAK, cell migration from embryoid bodies was inhibited, whereas alpha-myosin heav

287 wly developed loss-of-function technology in embryoid bodies, we find that Gata2 and Smad5 cooperate

288 n of this pathway in the primitive endoderm, embryoid bodies were cultured in the presence of a small

289 METHODS AND Embryoid bodies were derived from human keratinocytes, t

290 mRNA levels induced upon differentiation to embryoid bodies were down-regulated in homozygous null H

291 suppress cardiogenesis through Src kinases, embryoid bodies were exposed to the small molecule PP2,

292 organogenesis protocol was optimised whereby embryoid bodies were formed and patterned towards an eye

293 The outer cells of these embryoid bodies were found to gradually acquire the hall

294 e cloning efficiency and the ability to form embryoid bodies were restored in embryonic stem cells, i

295 ment membrane assembly was also evaluated in embryoid bodies where it was found that both LG1-3 and L

296 hen slowly decreased upon differentiation to embryoid bodies, whereas 5-methylcytosine levels increas

297 hat defined differentiation of ES cells into embryoid bodies with Activin-A and selection for T expre

298 They produce embryoid bodies with elevated levels of the primitive en

299 The number and the percentage of pig-a- embryoid bodies with hematopoietic differentiation, howe

300 Incubation of embryoid bodies with the vital dye, Dil, revealed the pe