ライフサイエンスコーパス: endogenous protein

1 ype protein and are able to compete with the endogenous protein.

2 scribe new PMEL17 antibodies that detect the endogenous protein.

3 al autoimmune encephalomyelitis (EAE) to the endogenous protein.

4 recover through open states from block by an endogenous protein.

5 e biochemical tools capable of targeting the endogenous protein.

6 t to an open-channel block and unblock by an endogenous protein.

7 using newly developed antibodies against the endogenous protein.

8 overexpressed by approximately 14-fold over endogenous protein.

9 completely dependent upon knocking down the endogenous protein.

10 nous Fis1 was expressed in yeast lacking the endogenous protein.

11 cells and recapitulates the behavior of the endogenous protein.

12 on and the nonspecific interactions with the endogenous proteins.

13 e are no systematic studies of ileal digesta endogenous proteins.

14 s from a variety of cellular contexts and to endogenous proteins.

15 ntracellularly and bacterially expressed and endogenous proteins.

16 expressed at levels several fold that of the endogenous proteins.

17 precipitation assays with both exogenous and endogenous proteins.

18 ronal and non-neuronal cells expressing only endogenous proteins.

19 sitivity to drugs but also interactions with endogenous proteins.

20 affect the interactions of microtubules with endogenous proteins.

21 cross talk, autofluorescence, and unlabeled, endogenous proteins.

22 100s to 1000s of simultaneous separations of endogenous proteins.

23 sed that methamphetamine covalently glycates endogenous proteins.

24 ells were consistent with the interaction of endogenous proteins.

25 ally and efficiently label multiple distinct endogenous proteins.

26 ting with and activating as-yet-unidentified endogenous proteins.

27 e fused activation or repression domain with endogenous proteins.

28 dimer because of potential dimerization with endogenous proteins.

29 pecificity and sensitivity to focus study on endogenous proteins.

30 and immunoblotted to determine expressed and endogenous proteins.

31 DNA is detected by co-immunoprecipitation of endogenous proteins.

32 competitors to the cognate sequences in the endogenous proteins.

33 nalogues, which in turn do not interact with endogenous proteins.

34 enoxyl radicals that covalently tag proximal endogenous proteins.

35 od to measure biomarkers, protein drugs, and endogenous proteins.

36 precipitation assays with both exogenous and endogenous proteins.

37 new perspectives for in-cell EPR studies of endogenous proteins.

38 ele encoding an epitope-tagged version of an endogenous protein, (3) alleles expressing reporter prot

39 1alpha reporter constructs as surrogates for endogenous protein activity and target expression, respe

40 Biosensors that report endogenous protein activity in vivo can be based on envi

41 we present Trim-Away, a technique to degrade endogenous proteins acutely in mammalian cells without p

42 nce (RNAi) resulted in complete knockdown of endogenous protein after 48 h, followed by cell death af

43 gregation in budding yeast, identifying >170 endogenous proteins aggregating within minutes of heat s

44 Transfer among labeled subunits revealed the endogenous protein alignment.

45 me-resolved quantitative confocal imaging of endogenous proteins along with a fluid-phase marker to a

46 vector may be used to fluorescently label an endogenous protein (alpha-tubulin).

47 uced end-joining activity independent of the endogenous protein amounts of DNA ligase IV, Ku80, and K

48 haromyces cerevisiae, a eukaryote that lacks endogenous protein AMPylation.

49 s verified by confocal immunofluorescence of endogenous protein and confirmed by AROS depletion using

50 Co-immunoprecipitation of synthetic and endogenous proteins and co-elution with gel filtration s

51 h generates full-length Citrine fusions with endogenous proteins and conditional mutants from a singl

52 s unclear precisely how macromolecules (e.g. endogenous proteins and exogenous immunotherapeutics) ac

53 f protein methylation from SDS-PAGE isolated endogenous proteins and identified complications for ass

54 Reciprocal immunoprecipitation (IP) of endogenous proteins and microscopically apparent overlap

55 e respiratory activity prevents digestion of endogenous proteins and RNA, which yields bacteria that

56 l-length MbSrc4 displays low activity toward endogenous proteins, and it cannot functionally substitu

57 IIC, isoform-specific immunostaining of the endogenous proteins, and two-color total internal reflec

58 sis of the rates at which newly synthesized, endogenous proteins appear within mammalian nucleoli.

59 Several endogenous proteins are capable of inhibiting the cataly

60 beling reveals that even severely aggregated endogenous proteins are disaggregated without degradatio

61 The endogenous proteins are expressed in separate chromaffin

62 Modified endogenous proteins are immunogenic, and specific antibo

63 of the Ov-SPI proteins demonstrates that the endogenous proteins are localized to the basal layer of

64 cherichia coli biotin ligase (BirA), whereas endogenous proteins are not modified.

65 ble in 500 mM NaCl, whereas rods formed from endogenous proteins are significantly less stable in hig

66 tracted neuron recruitment has promoted this endogenous protein as a popular indirect tool to monitor

67 reveal a unique molecular code that defines endogenous proteins as inflammatory stimuli by marking t

68 neuploid strains are prone to aggregation of endogenous proteins as well as of ectopically expressed

69 orozoite arising from epitope tagging of the endogenous protein) as a key regulator for adhesion duri

70 interest to apply native MS to the study of endogenous protein assemblies-i.e., those wherein the co

71 oichiometry and intersubunit connectivity of endogenous protein assemblies-information that is key to

72 is phosphorylated by Nek8 in vitro, and the endogenous proteins associate in vivo.

73 nd tissue provides precise quantification of endogenous proteins at the approximately 10 pg/mL level

74 rged residue is a SecYEG determinant for the endogenous proteins ATP synthase subunits a and b and th

75 nformational changes or other alterations in endogenous protein autoantigens are required for recogni

76 atinib-directed NEDDylation occurs for known endogenous protein binders in complex cell lysates.

77 acids are disguised from recognition by the endogenous protein biosynthetic machinery, effectively p

78 onship is not only confirmed at the level of endogenous proteins but is also supported by a physical

79 e colors for imaging interaction networks of endogenous proteins, but also hold the potential to prov

80 nt of the fluorescent protein is fused to an endogenous protein by in-frame exon traps in the enhance

81 protein is predominately cytoplasmic, as is endogenous protein by subcellular fractionation.

82 by luciferase assay and the response of the endogenous protein by Western blot in HeLa cells transie

83 The technique, single-cell labeling of endogenous proteins by clustered regularly interspaced s

84 We also observed phosphorylation of endogenous proteins by endogenous IP7 in yeast.

85 mice and factor V Leiden mice), in which the endogenous protein C anticoagulant pathway is disrupted

86 scular blood coagulation (thrombosis) by the endogenous protein C system.

87 In addition, the endogenous proteins can be isolated as a complex by immu

88 ugh autoantibodies are generated against the endogenous protein, CD4 cells are activated through a ne

89 ce nanopores of a size comparable to that of endogenous protein channels, but replicating their affin

90 Endogenous protein citrullination was abundant in P ging

91 or example, acetylation and methylation) and endogenous protein cleavages, especially when combinatio

92 ubunit of the mitochondrial ribosome and the endogenous protein co-fractionated with the 28S subunit

93 Only minor amounts of endogenous proteins coaggregated with IMiQ deposits and

94 Endogenous protein-coding gene and transposon transcript

95 mental approach, we identified and validated endogenous protein-coding transcripts that regulate PTEN

96 Quantitative analysis of endogenous protein colocalization in growth cones after

97 ol, membrane and cellular organelles, and to endogenous protein complexes from animal tissue extracts

98 plication of native MS to the elucidation of endogenous protein complexes has been limited by the dif

99 es and mass spectrometry, allow the study of endogenous protein complexes in Drosophila.

100 Targeting endogenous protein complexes of higher eukaryotes, parti

101 hod allowing simultaneous global analysis of endogenous protein complexes that begins with intact lea

102 es rapid and efficient affinity isolation of endogenous protein complexes with a sensitive native MS

103 of either tagged fusion protein complexes or endogenous protein complexes, illustrated here using the

104 purification and identification of extended endogenous protein complexes.

105 the host protein in the context of different endogenous protein complexes.

106 Endogenous protein concentrations were determined by com

107 TPase subunit of human SWI/SNF (hSWI/SNF) at endogenous protein concentrations.

108 Here, we repurpose an endogenous protein degradation system to rapidly remove

109 D-tagged protein can functionally replace an endogenous protein depleted by RNAi, leading to an induc

110 hat cell type-specific nanoscale analysis of endogenous protein distribution is possible in brain cir

111 these ORF proteins were used to localize the endogenous proteins during chlamydial infection.

112 t preventing crosslinking within and between endogenous proteins during hydrogel-tissue hybridization

113 em was used to ablate expression of specific endogenous proteins (e.g., TRAF6; Rb), and was shown to

114 In the presence of the endogenous protein, ectopic Reelin did not alter cell mi

115 odent malaria parasite, P. berghei, in which endogenous proteins engineered to contain the auxin-indu

116 The purpose of this study was to identify endogenous protein enhancers using yeast two-hybrid, co-

117 mph DAFPs as "bait" in an effort to identify endogenous protein enhancers.

118 e also introduce a new method for decreasing endogenous protein expression in neurons.

119 of transiently transfected 293T cells and at endogenous protein expression levels in MDA-MB-435 breas

120 we identified multiple miRNAs that repressed endogenous protein expression of both genes, seed-based

121 t these same sites are phosphorylated in the endogenous protein extracted from Limulus lateral eye, a

122 ntial sources, as follows: newly synthesized endogenous proteins for direct presentation on the surfa

123 the cell surface, derived from virtually all endogenous proteins, for inspection by cytotoxic T cells

124 hnique, we are able to examine low levels of endogenous protein from cell extracts without the need f

125 ulation is accompanied by a depletion of the endogenous protein from its normal localization.

126 e when expressed in vitro, extraction of the endogenous protein from membranes requires detergents.

127 phosphopeptides by UV irradiation displaced endogenous proteins from 14-3-3-binding, causing prematu

128 was demonstrated by coimmunoprecipitation of endogenous proteins from brain tissue and shown to be di

129 jA was confirmed by coimmunoprecipitation of endogenous proteins from flies, and the endogenous prote

130 Using specific antibodies to detect endogenous proteins from human and mouse cells, we found

131 as confirmed by coimmunoprecipitation of the endogenous proteins from human HepG2 or CaSki cells, alt

132 Interaction proteomics analysis of endogenous proteins from the cellular model, mainly in h

133 inducible, and allele-targeted inhibition of endogenous protein function, and the ability to titrate

134 n or transiently (within 1 h) by tagging the endogenous protein genomically with the auxin degron.

135 Drug covalent binding to endogenous proteins (haptenation) is thought to be requi

136 The endogenous protein has 88.0% sequence homology with the

137 thods faithfully report on the properties of endogenous proteins has not been studied comparatively.

138 owever, extracellular morphogen gradients of endogenous proteins have not been directly visualized in

139 Proteomic analysis of rods formed from endogenous proteins identified other potential component

140 faithfully mimicked the distribution of the endogenous protein in both interphase and mitosis.

141 otting, corresponding to the localization of endogenous protein in cytoplasmic vesicular structures b

142 The presence of endogenous protein in dinosaur bone may validate hypothe

143 SNX2, we first examined the distribution of endogenous protein in HeLa cells.

144 sfected COS-7 and PC12 cells and also of the endogenous protein in rat forebrain slices.

145 correlates with retarded degradation of the endogenous protein in wild-type plants and prolonged epi

146 or analysis and the ability to work with the endogenous proteins in a given sample (e.g., stabile iso

147 s show relatively little cross-reaction with endogenous proteins in a variety of monocotyledonous and

148 In this study, by fluorescently labeling endogenous proteins in Caenorhabditis elegans embryos, w

149 scribe a method for absolute quantitation of endogenous proteins in cell lysates using an automated c

150 nslated BIG2 and RI alpha, as well as of the endogenous proteins in cytosol of cultured HepG2 cells.

151 ins the measured robustness of synthesis for endogenous proteins in E. coli.

152 The interactions with endogenous proteins in human cell extracts were confirme

153 dy the physical and biological properties of endogenous proteins in human cells without the need for

154 o-immunoprecipitation in Cos7 cells and with endogenous proteins in human neuroblastoma cells.

155 an embryonic kidney (HEK) 293 cells and with endogenous proteins in human neuroblastoma cells.

156 unoprecipitation in mammalian cells and with endogenous proteins in JEG3 placental cells, neuroblasto

157 ormat, small-molecule target engagement with endogenous proteins in live cells can be quantified.

158 The ability to visualize endogenous proteins in living neurons provides a powerfu

159 munoprecipitation/Western blot analysis with endogenous proteins in MEL cells and heterologous protei

160 s strategy, we achieved efficient tagging of endogenous proteins in primary and organotypic cultures

161 quilin 1 interaction can be detected between endogenous proteins in primary neurons in vitro as well

162 periments demonstrate an interaction between endogenous proteins in rat brain cytosol.

163 lutaminases cross-linked this substrate into endogenous proteins in situ; an enzyme-linked immunosorb

164 ient transfections in cells, and analysis of endogenous proteins in stably transfected Madin-Darby ca

165 e absolute quantification of therapeutic and endogenous proteins in the biopharmaceutical industry ov

166 with the finding that association of the two endogenous proteins in the cell extract chromatin fracti

167 We found that interaction of the two endogenous proteins in the differentiated muscle fibers

168 st (>35-fold difference in affinity) and for endogenous proteins in tissue culture cells.

169 ion of the localization and dynamics of many endogenous proteins in various cell types, regions, and

170 This interaction was confirmed for endogenous proteins in vivo using immunoprecipitation an

171 Wnt activity is antagonized by endogenous proteins including dickkopf-1 (DKK-1).

172 This is also a route used by endogenous proteins, including GPP130, which is an integ

173 egral sarcolemmal phosphoprotein, is a novel endogenous protein inhibitor of cardiac NCX1.

174 TopAI is the first endogenous protein inhibitor specific for topoisomerase

175 tivity is posttranscriptionally regulated by endogenous protein inhibitors (PMEIs).

176 e association of PP1 catalytic subunits with endogenous protein inhibitors and regulatory subunits.

177 A family of endogenous protein inhibitors of calcineurin was recentl

178 Cystatins such as cystatin M are potent endogenous protein inhibitors of lysosomal cysteine prot

179 d reporter polypeptides as well as misfolded endogenous proteins inside mitochondria takes place via

180 ting enzyme UBE2D3 with substrate PCNA), and endogenous proteins interacting with thioredoxin in E. c

181 eins of interest, enabling the monitoring of endogenous protein interactions or detection of a single

182 complementation to be expanded to monitoring endogenous protein interactions.

183 enerative disease caused by misfolding of an endogenous protein into an alternative pathogenic confor

184 aggregation-prone proteins can convert other endogenous proteins into drivers of aggregation and toxi

185 The conversion of exogenous and endogenous proteins into immunogenic peptides recognized

186 ers characterized by the aberrant folding of endogenous proteins into self-propagating pathogenic con

187 express GFP in the normal tissues where the endogenous protein is present.

188 identify precise subcellular localization of endogenous proteins is essential for integrative underst

189 d peptides with phosphopeptides derived from endogenous proteins isolated from cells in which the kin

190 The data show further that endogenous protein kinase A is a primary catalyst of cel

191 The expression of endogenous protein kinase C (PKC) isoforms in HEK293 cel

192 ase C competitively with diacylglycerol, the endogenous protein kinase C regulator, and plant-derived

193 ice, including: differences in regulation by endogenous protein kinase C, differential sensitivity to

194 Changes in RUNX1 activity affect endogenous protein kinase Cbeta expression, and a domina

195 Endogenous protein kinase CK2 purifies with Bdf1 and pho

196 regulation of PKA signaling, members of the endogenous protein kinase inhibitor (PKI) family may be

197 Our data suggest that: (1) multiple endogenous protein kinases and phosphatases functionally

198 vity of BK channels is regulated by multiple endogenous protein kinases and protein phosphatases in t

199 ive protein, which cannot be switched off by endogenous protein kinases.

200 hibited by okadaic acid, and was restored by endogenous protein kinases.

201 s that the protein is no longer regulated by endogenous protein kinases.

202 nts from the nanoparticle surface results in endogenous protein knockdown simultaneously with exogeno

203 Bid, an endogenous protein known to permeabilize mitochondrial m

204 S3 and Smad3 interact with each other at the endogenous protein level in mammalian cells and also in

205 Endogenous protein levels for these Wnt antagonists are

206 A method that directly alters endogenous protein levels is currently not available.

207 ow that Pyk2 increases promoter activity and endogenous protein levels of involucrin, a marker of ker

208 or NHERF2 caused a significant reduction in endogenous protein levels of SR-BI.

209 In muscles from Cmpt mice, endogenous protein levels of STIM1 and Orai1 were reduce

210 y reduced induction of promoter activity and endogenous protein levels of the CREB-dependent gene, c-

211 ciate with Aph-1 at overexpressed as well as endogenous protein levels.

212 Drosophila Toll receptor is activated by the endogenous protein ligand Spatzle in response to microbi

213 es at the proximal heme face in place of the endogenous protein ligand.

214 , using quadruple immunolocalization against endogenous proteins, live imaging of dendritic endosomes

215 We examined endogenous protein localization within the framework of

216 co-immunoprecipitation of overexpressed and endogenous proteins, maps to the amino-terminal 70 resid

217 d is essential for understanding why and how endogenous proteins may adopt a non-native fold.

218 convenient probe to study the activities of endogenous protein methyltransferases.

219 Our findings raise the possibility that endogenous protein modifications that disrupt the M80-Fe

220 ate the mechanisms and the kinetics of these endogenous protein modifications, we used a dynamic arti

221 relevant to unravel mechanisms making other endogenous proteins more vulnerable to undergo conformat

222 he molecular mechanism of TLR9 activation by endogenous protein-nucleic acid complexes, which are ass

223 st, lysosomal trafficking of endocytosed and endogenous proteins occur via different routes and/or ar

224 ty, we studied the effects of overexpressing endogenous proteins on disulphide bond formation in the

225 med cells must present peptides derived from endogenous proteins on MHC class I molecules to be recog

226 ty biotinylation, we captured and identified endogenous proteins on the outer mitochondrial membrane

227 yBL is extremely sequence-specific, as endogenous proteins on the surface of yeast and HeLa cel

228 gs without inhibiting the Ag presentation of endogenous protein or peptides.

229 Here we introduce new approaches to quantify endogenous protein organization at single-molecule resol

230 Cytosolic peptidases degrade endogenous proteins originating from self or pathogens,

231 BSco) has a histidine (H135) and an unknown endogenous protein oxygen ligand in a distorted tetragon

232 Unexpectedly, delivery of endogenous proteins, p67 and TbCatL, were unaffected ind

233 uggest that, despite the lack of full-length endogenous protein, patients with PTC-inducing non-sense

234 (flg22), elongation factor-Tu (elf18), or an endogenous protein (pep1).

235 Understanding why the endogenous protein PF4 becomes immunogenic when complexi

236 pha-4 coprecipitated hemagglutinin-MEK3 plus endogenous protein phosphatase 2A (PP2A) and selectively

237 ls to the extracellular matrix, and we found endogenous protein phosphatase-1alpha isoform (PP1alpha)

238 in a hippocampal cell line, with >60% of all endogenous protein phosphorylated at this residue.

239 synthesis and receptor components with yeast endogenous protein phosphorylation elements and new resp

240 The snake venom protein rhodocytin and the endogenous protein podoplanin have been identified as li

241 -[(32)P]GMP detected an approximately 47-kDa endogenous protein, presumably c-di-GMP downstream recep

242 hroughput quantitative mass spectrometry and endogenous protein-protein interaction revealed an NLRX1

243 of selectively altering the distribution of endogenous proteins, providing a link between intronic S

244 Endogenous protein quality control machinery has long be

245 by biotin ligase added to the medium, while endogenous proteins remain unmodified.

246 ose tissue seven-, five-, and threefold over endogenous protein, respectively.

247 e Erv1 into erv1-ts mitochondria lacking the endogenous protein restores import and assembly of the s

248 orbital-lowering intermediate similar to the endogenous protein rhodopsin effectively catalyzes isome

249 pid immunoprecipitation mass spectrometry of endogenous protein (RIME) is a method that allows the st

250 for a sufficient time to reduce the level of endogenous protein, should be generally useful for targe

251 cently tagged proteins and immunostaining of endogenous proteins showed that NM2 and M18A are present

252 rinary elimination, based on manipulating an endogenous protein:siderophore:iron clearance pathway.

253 , whereas the latter can detect thousands of endogenous proteins simultaneously, but only in lysed sa

254 metics, because they mimic the action of the endogenous protein Smac/Diablo that antagonizes cIAP fun

255 cross-reactions between externally added and endogenous protein species.

256 e predicted factor as a new 1,277-amino-acid endogenous protein (STAMP).

257 tdIns 3-P at the 5-hydroxyl and unidentified endogenous protein substrate(s).

258 were found to prevent BoNT/A cleavage of the endogenous protein substrate, SNAP-25, even at low muM c

259 e site, thus impeding its proteolysis of the endogenous protein substrate, synaptosomal-associated pr

260 Diseases associated with the misfolding of endogenous proteins, such as Alzheimer's disease and typ

261 r medically relevant imaging because certain endogenous proteins, such as muscle myosin lattices with

262 Gel filtration of the endogenous protein suggested that its predominant struct

263 Conversely, reducing endogenous protein sumoylation with dominant-negative SU

264 man cryosectioned podocytes may reflect both endogenous protein synthesis and APOL1 uptake from the c

265 imaging also revealed specific inhibition of endogenous protein synthesis in the SAT1 overexpressing

266 ter, as well as a small-molecule reporter of endogenous protein synthesis, we show that dopamine D1/D

267 are not substrates for SAT1 restored GFP and endogenous protein synthesis.

268 These peptides can be generated from either endogenous proteins synthesized by the biosynthetic mach

269 In an endogenous protein tagging assay, lon mutants accumulate

270 We have demonstrated dual-color endogenous protein tagging with sfCherry211 and GFP11, r

271 RNA tag and extensive ladder-like pattern of endogenous protein-tagging events in F. tularensis that

272 We use this to determine the effects of endogenous protein tags or drugs on the shape dynamics o

273 o to one or more of the same mt sites as the endogenous protein that includes the matrix.

274 vitro and in vivo, with alpha-synuclein, an endogenous protein that is implicated in pathology of PD

275 High molecular weight kininogen (HK) is an endogenous protein that is proteolytically cleaved in pl

276 The existence of endogenous proteins that act as "anti-chaperones" uncove

277 of mitochondria, with cell death executed by endogenous proteins that act to increase the permeabilit

278 e catalyzes the biotinylation of neighboring endogenous proteins that can then be isolated and identi

279 nase-2 (TIMP-2) belongs to a small family of endogenous proteins that inhibits a group of enzymes, th

280 Interactions are confirmed for chimeric and endogenous proteins through quantitated in vitro and cel

281 reporter constructs and Western blotting of endogenous protein to verify that miR-125b targets Suv39

282 HPV type 16 (HPV16) E6 interacts with endogenous proteins to activate hTERT, the catalytic sub

283 A-I in humans) present peptides derived from endogenous proteins to CTLs.

284 ncentration is a function of dietary intake, endogenous protein turnover and catabolism of the three

285 further supported by co-localization of the endogenous proteins using confocal microscopy.

286 ecipitation and proximity ligation assays on endogenous proteins, we demonstrate that Parkin interact

287 In this study using purified endogenous proteins, we examined the interaction between

288 s from WT and telokin KO mice as a source of endogenous proteins, we found that only in the presence

289 In previous work with endogenous proteins, we identified that Sac3, a phosphat

290 n of endogenous proteins from flies, and the endogenous proteins were found to colocalize at the trac

291 animals express very low levels of VMAT2, an endogenous protein which sequesters monoamines intracell

292 alance between immunological clearance of an endogenous protein with acquired toxic properties and th

293 These two models were tested by replacing endogenous protein with ZP2 that cannot be cleaved (Zp2(

294 enome-editing strategy to systematically tag endogenous proteins with fluorescent tags in human induc

295 simple and generalizable technique to image endogenous proteins with high specificity, resolution, a

296 D), which can be used to fluorescently label endogenous proteins with near ideal properties in all ne

297 receptors and protease-activated receptors, endogenous proteins with protective effects such as the

298 It combines RNAi-mediated silencing of two endogenous proteins with the expression of two exogenous

299 tform to map the subcellular localization of endogenous proteins with the resolution of micro- to nan

300 ly and temporally resolved proteomic maps of endogenous proteins within living cells.