ライフサイエンスコーパス: endoglucanase

1 degradation of cellulose in the presence of endoglucanase.

2 off-rates, processivity, and synergism with endoglucanase.

3 y 9 of the glycosidases, suggesting it is an endoglucanase.

4 s mixtures of commercially relevant exo- and endoglucanases.

5 s unusual in its abundance of GH5-containing endoglucanases.

6 llulosome as well as the previously reported endoglucanases.

7 In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creatin

8 in xyloglucan composition were larger in the endoglucanase-accessible fraction.

9 of EngB and EngD without compromising their endoglucanase activities.

10 raction with CESAs in vitro, suggesting that endoglucanase activity is important for cellulose synthe

11 secrete two beta1,3-glucanases and that Eng1 endoglucanase activity is the predominant factor respons

12 GH9A1(A577V) abolishes the endoglucanase activity of GH9A1 in vitro but does not af

13 f soybean, and that specifically inhibit the endoglucanase activity of their plant host.

14 lely as transglycosylases because xyloglucan endoglucanase activity was not apparent.

15 ough thin-layer chromatography indicated its endoglucanase activity.

16 nd, importantly, chloroplast extracts showed endoglucanase activity.

17 lucanases are functionally equivalent to the endoglucanases and cellobiohydrolases required for other

18 noncomplexed cellulolytic system composed of endoglucanases and cellobiohydrolases.

19 nificant differences between hydrolysis with endoglucanases and cellulase mixtures were observed.

20 ch also comprises a broad range of microbial endoglucanases and endogalactanases, as well as yeast ce

21 her purified NEC4 was able to inhibit fungal endoglucanases and xylanases.

22 PhcA-regulated factors exopolysaccharide I, endoglucanase, and pectin methyl esterase was reduced 10

23 is protein is uncommon for hyperthermophilic endoglucanases, and two of the four domains of the enzym

24 The processive endoglucanases are functionally equivalent to the endogl

25 ed in GH7 cellobiohydrolases, but not in GH7 endoglucanases, at the leading glucosyl ring provide the

26 form a complex with cellulosomal cellulases endoglucanase B (EngB) and endoglucanase L (EngL).

27 erins, it was also shown that the binding of endoglucanase B (EngB) to CbpA was dependent on the pres

28 oint mutation in the KORRIGAN (KOR) beta,1-4 endoglucanase (beta,1-4 EGase) gene.

29 d from EcCel5A, a Eubacterium cellulosolvens endoglucanase, bind to a range of beta-glucans but, uniq

30 n of a recoverable, thermoresponsive polymer-endoglucanase bioconjugate that matches the activity of

31 Cel6A was shown to be a classic endoglucanase, but Cel5H showed significantly higher act

32 ucture of the catalytic core of the family 5 endoglucanase, Ce15A, from the alkalophilic Bacillus aga

33 A cellulose-specific endoglucanase (CEG from Aspergillus niger) did not cause

34 vated segments in alpha-expansin or a fungal endoglucanase (Cel12A).

35 a double-dockerin construct from the P. equi endoglucanase Cel45A.

36 Endoglucanase Cel5A from Bacillus agaradhaerens, classif

37 f this strategy, we isolated variants of the endoglucanase Cel5A, from the plant-pathogenic fungus Fu

38 This is exemplified by endoglucanase Cel5A, which has three internal family 6 c

39 ated that three of the 12 predicted beta-1,4 endoglucanases (cel5A, cel5B, and cel45A) and the sole p

40 Endoglucanase CelB from Streptomyces lividans performs h

41 ural synthetic enzymatic pathway composed of endoglucanase, cellobiohydrolyase, cellobiose phosphoryl

42 omic organization of genes encoding beta-1,4-endoglucanases (cellulases) from the plant-parasitic cys

43 Although two endoglucanases (CelZ and CelY; formerly EGZ and EGY) are

44 module from the Bacillus sp. 1139 family GH5 endoglucanase, comprising a 191 amino acid protein, has

45 ith additional enzymes, including a family-5 endoglucanase, confirmed the conclusion that to cause cr

46 an unusual family 9 enzyme, is a processive endoglucanase containing a catalytic domain closely link

47 Endoglucanase D (EngD) from Clostridium cellulovorans is

48 The chromatographic pattern of endoglucanase digested beta-1,6-glucan provides a charac

49 CBM_E1, which is linked to an endoglucanase, displayed affinity for mixed linked beta1

50 ression of a bacterial gene, celE', encoding endoglucanase E' (EGE'), was investigated in the pancrea

51 engE, coding for endoglucanase E, one of the three major subunits of the

52 The gene engE, coding for endoglucanase E, one of the three major subunits of the

53 I, CBH II) and an internally chain-cleaving endoglucanase (EG), the major components of cellulase sy

54 lso report the identification of two soybean endoglucanases: EGaseA, which acts as a high-affinity li

55 The reversible folding of an endoglucanase (EGIII) from the filamentous fungus Tricho

56 The thermostable endoglucanase EGPh from the hypothermophilic Pyrococcus

57 ion of the synergism between randomly acting endoglucanases (EGs) and chain end-specific processive c

58 regulation of the endochitinase Cts1 and the endoglucanase Egt2 by Swi5.

59 is study, we enhanced the thermostability of endoglucanase EngB, one component of the cellulase compl

60 t recombinant cellulosomes containing either endoglucanase EngE, endoglucanase EngH, or exoglucanase

61 osomes containing either endoglucanase EngE, endoglucanase EngH, or exoglucanase ExgS bound to mini-C

62 ne in Clostridium cellulovorans that encodes endoglucanase EngL, which is involved in plant cell wall

63 eotide sequence contained reading frames for endoglucanase EngO, a putative response regulator, and a

64 ns produces a major noncellulosomal family 9 endoglucanase EngO.

65 Each of the R. albus 8 endoglucanases, except for Ra0259 and Ra0325, bound to t

66 ty assays mapped the distribution pattern of endoglucanase, exoglucanase and xylanase activity throug

67 e significant sequence similarity with known endoglucanases, exoglucanases and xylanases.

68 dation requires a suite of enzymes including endoglucanases, exoglucanases, and beta-glucosidases.

69 uctural analysis of a unique, mixed function endoglucanase from black cottonwood (Populus trichocarpa

70 The eglA gene, encoding a thermostable endoglucanase from the hyperthermophilic archaeon Pyroco

71 ese findings on a model cellulase enzyme, an endoglucanase from the thermophilic Pyrococcus horikoshi

72 catalytic nucleophile and proton donor, with endoglucanases from glucosyl hydrolase family 12.

73 lostridium cellulovorans (strain ATCC 35296) endoglucanase gene engF has been isolated and sequenced.

74 The endoglucanase has a 19-amino-acid signal peptide but not

75 The P. furiosus endoglucanase has significant amino acid sequence simila

76 Purified endoglucanases have been used to determine the compositi

77 Here, we focus on endoglucanase I (Cel7B) from the fungus Trichoderma rees

78 amylase, the secretion of Trichoderma reesei endoglucanase I (EGI) was not influenced by the YlPMR1 d

79 Our study establishes the important role of endoglucanase in cellulose synthesis and cellulose micro

80 be similar to the xyloglucan-specific fungal endoglucanase inhibitor protein (XEGIP) precursor in tom

81 Furthermore, GIPs and soybean endoglucanases interact in vivo during pathogenesis in s

82 osomal cellulases endoglucanase B (EngB) and endoglucanase L (EngL).

83 unique polymer is hydrolyzed by the specific endoglucanase lichenase, but, unlike lichenan and barley

84 other enzymes, strongly suggesting that dual endoglucanase-mannanase activity is widespread in this f

85 Putative glycohydrolases and an endoglucanase may enable catabolism of (hemi)cellulose i

86 portions of ORFs denoted in M. jannaschii as endoglucanase (MJ0555), transketolase (MJ0681), thiamine

87 ly regulated plant genes encoding a beta,1-3 endoglucanase (MtBGLU1), a lectin (MtLEC4), and a cystei

88 onfirmed the conclusion that to cause creep, endoglucanases must cut both xyloglucan and cellulose.

89 the activities of exocellobiohydrolases and endoglucanases needs to be re-appraised in both species.

90 protein CbpA, the exoglucanase ExgS, several endoglucanases of family 9, the mannanase ManA, and the

91 ve 2.5-fold improvement in the display of an endoglucanase on the yeast surface by optimizing multipl

92 hermore, silencing of the elicitor-releasing endoglucanase (PR-2) led to a loss of HR cell death and

93 ogen-secreted apoplastic xyloglucan-specific endoglucanase, PsXEG1, is a focus of this struggle in th

94 1831, Ra2461, and Ra2535) were identified as endoglucanases, releasing predominantly cellobiose and c

95 We have also identified an insoluble endoglucanase-resistant type of 1,3-linked glucan presen

96 Immunolocalization of a secreted endoglucanase revealed that proteins are secreted mainly

97 Phylogenetic analysis of the endoglucanases revealed three distinct subfamilies of gl

98 s the surface, as has been observed in other endoglucanase structures, and is potentially able to acc

99 failed to induce cell wall creep, whereas an endoglucanase that hydrolyzes both xyloglucan and cellul

100 upstream of a gene encoding a family 9 type endoglucanase that we have designated as EngH.

101 reviously identified plant EGases are E-type endoglucanases that possess signal sequences for endopla

102 tically with a cognate cellobiohydrolase and endoglucanase to completely release, from a cellulosic s

103 icroscopy (AFM) with a stretching device and endoglucanase treatment that induces wall stress relaxat

104 M) with nanogold affinity tags and selective endoglucanase treatments to assess the spatial location

105 ding a serine protease, and engXCA, encoding endoglucanase) was reduced in the rpfA mutant background

106 cellulases and to a single putative beta-1-4 endoglucanase were expressed at high levels relative to

107 llobiohydrolase Cel6A and the GH5-containing endoglucanases were evaluated.

108 hibitor of a family GH12 xyloglucan-specific endoglucanase with a K(i) of 0.35 nm.

109 rCel7A is a cellobiohydrolase rather than an endoglucanase, with a cellulose-binding tunnel that is m

110 Xyloglucan-specific endoglucanase (XEG from Aspergillus aculeatus) failed to

111 hydrolytic activity of a xyloglucan-specific endoglucanase (XEG) from the fungus Aspergillus aculeatu

112 by treatment of the walls with a XG-specific endoglucanase (XEG).