ライフサイエンスコーパス: endoglycosidase H

1 mmature form was sensitive to digestion with endoglycosidase H.

2 se and O-glycanase but not to treatment with endoglycosidase H.

3 ome cells, it is sensitive to digestion with endoglycosidase H.

4 as assessed by acquisition of resistance to endoglycosidase H.

5 ecular weight form and gained sensitivity to endoglycosidase H.

6 and only the 93-kDa wt C2 were sensitive to endoglycosidase H, a marker for transport from the endop

7 Digestion of gp120 with endoglycosidase H abrogates the binding of SP-A, indicat

8 Pulse-chase and endoglycosidase H analysis demonstrate that the iHeps re

9 Cell surface biotinylation and endoglycosidase H analysis revealed a 128-kDa precursor

10 Endoglycosidase H analysis revealed that the modified En

11 cognized by this antiserum were sensitive to endoglycosidase H and associated with calnexin, indicati

12 igomers; however, they remained sensitive to endoglycosidase H and did not undergo endoproteolytic cl

13 were established by pulse-chase analysis and endoglycosidase H and glycopeptidase F digestions.

14 Digestion with N-glycosidase F, endoglycosidase H and lectin blotting did not reveal any

15 Endoglycosidase H and peptide N-glycosidase treatment an

16 nous parasite glycoproteins using sequential endoglycosidase H and peptide:N-glycosidase-F digestions

17 e and mature GC-C glycoforms on the basis of endoglycosidase H and PNGase sensitivities.

18 US7, US9, and US10 remained sensitive to endoglycosidase H and were exclusively or largely presen

19 s a 45-kDa glycoprotein that is sensitive to endoglycosidase-H and is reduced to 37-kDa after N-glyca

20 tions of N-glycosidase F, endoglycosidase F, endoglycosidase H, and endo-beta-galactosidase.

21 ith neuraminidase, resistance to cleavage by endoglycosidase H, and GalNAc-independent labeling with

22 mutant receptor to peptide-N-glycanase F and endoglycosidase H, and insensitivity to sialidase indica

23 138 and Asn-175 were completely sensitive to endoglycosidase H, and they were phosphorylated.

24 porate the J chain and resist degradation by endoglycosidase H, arguing for IgM passage through the G

25 CPD increases to 180 kDa and is resistant to endoglycosidase H as a result of carbohydrate maturation

26 PP I proenzyme with both N-glycosidase F and endoglycosidase H as well as treatment of the cells with

27 amycin, endoglycosidase F/N-glycosidase, and endoglycosidase H but not to O-glycosidase.

28 , and both mature proteins were resistant to endoglycosidase H but sensitive to glycopeptidase F.

29 from mPmel17 not only in its sensitivity to endoglycosidase H, but also in the content of core 1 O-g

30 Treatment with endoglycosidase H caused an increase in electrophoretic

31 Endoglycosidase H cleaved the 45-kDa band to approximate

32 Effects of tunicamycin and endoglycosidase H confirmed that these novel isoforms re

33 y limited trypsin digestion and treated with endoglycosidase H deglycosidase to reduce heterogeneity

34 ecular mass in SDS-PAGE and was resistant to endoglycosidase H deglycosidase.

35 Peptide N-glycosidase F and endoglycosidase H deglycosylate hENT1 to 37 kDa and hENT

36 but in alpha1(A322D)beta2gamma2S receptors, endoglycosidase H deglycosylated 91 +/- 4% of alpha1(A32

37 In alpha1beta2gamma2S receptors, endoglycosidase H deglycosylated only 26 +/- 5% of alpha

38 In this study, we describe an endoglycosidase H-deglycosylated form of TPP1 containing

39 The structures, determined at 1.85 A for the endoglycosidase H-deglycosylated protease domain produce

40 n the Cbeta domain, was thrombin-cleaved and endoglycosidase H-digested, and the two derivatives were

41 However, cell surface biotinylation and endoglycosidase H digestion assays appear to under-repre

42 Endoglycosidase H digestion assays indicated that SALM1D

43 n sensitivity to both mannan competition and endoglycosidase H digestion compared to that of the 124m

44 Pulse-chase analysis and endoglycosidase H digestion of surface-biotinylated MMP-

45 Endoglycosidase H digestion of the mutated TfRs indicate

46 Endoglycosidase H digestion showed that a significant fr

47 on of flow cytometry, patch clamp recording, endoglycosidase H digestion, brefeldin A treatment, and

48 Endoglycosidase H digestion, immunocytochemistry, and su

49 elet Mpl, PV platelet Mpl was susceptible to endoglycosidase H digestion, indicating defective Mpl pr

50 gglutinin at a stage preceding resistance to endoglycosidase H digestion, thus impairing hemagglutini

51 Peptide N-glycosidase F, but not endoglycosidase H, digestion converted all beta1 to an a

52 Endoglycosidase-H digests and colocalization with endopl

53 Treatment with PNGase F, but not Endoglycosidase H (Endo H) (specific for high-mannose N-

54 was determined by proteinase K digestion and endoglycosidase H (Endo H) cleavage.

55 lex (pgCIII) was sensitive to treatment with endoglycosidase H (endo H), while the mature gCIII compl

56 ted with the presence of a limited amount of endoglycosidase H (Endo H)-resistant HLA-F.

57 pike protein (S) was newly synthesized as an endoglycosidase H (endo H)-sensitive glycoprotein (gp170

58 n-19 is not utilized; Asn-39 is linked to an endoglycosidase H (Endo H)-sensitive oligosaccharide, an

59 alnexin), yet surface TPO molecules remained endoglycosidase H (endo H)-sensitive.

60 dases peptide-N-glycosidase F (PNGase F) and endoglycosidase H (Endo H).

61 s, the N-linked sugars remained sensitive to Endoglycosidase H (Endo H).

62 ficantly reduced in cd81(-/-) B cells and is endoglycosidase H (endo-H) resistant.

63 Endoglycosidase H (endo-H) treatment of particles demons

64 However, endoglycosidase H (endoH) digestion of the viral envelop

65 onnatural sugars at N-glycan core positions, endoglycosidase H (endoH)-digested peptides from a purif

66 nt molecular weights and remained completely endoglycosidase H (endoH)-sensitive, suggesting incomple

67 Experiments employing endoglycosidase H indicated that the early slow processi

68 The acquisition of resistance to endoglycosidase H indicated trafficking of S to the medi

69 Yet, endoglycosidase H insensitivity indicates an aberrant ol

70 treatment of target cells with tunicamycin, endoglycosidase H, or protease (pronase).

71 By monitoring acquisition of endoglycosidase H resistance after metabolic labeling, w

72 tution mutant S proteins displayed levels of endoglycosidase H resistance and cell surface expression

73 se media reversibly inhibited development of endoglycosidase H resistance and mannose-binding activit

74 ldin A required more than 120 min to acquire endoglycosidase H resistance and maximal binding activit

75 slational processing of these two mutants to endoglycosidase H resistance and very low cell surface e

76 e cell surface have the potential to acquire endoglycosidase H resistance as a consequence of Golgi-b

77 he ER is confirmed by the failure to acquire endoglycosidase H resistance at 37 degrees C and cannot

78 d with a 45-min half-time for development of endoglycosidase H resistance.

79 ling studies demonstrate that acquisition of endoglycosidase-H resistance of CD1d is observed in the

80 val, (ii) all detectable processed mMDC15 is endoglycosidase H -resistant, and (iii) a recombinant so

81 ecules are delayed in their maturation to an endoglycosidase H-resistant form after biosynthetic labe

82 ed ADAM3 exhibited an increased amount of an endoglycosidase H-resistant form that may be related to

83 nverted to the Golgi-processed and therefore endoglycosidase H-resistant form, and the endoglycosidas

84 he Ostalpha subunit to a mature glycosylated endoglycosidase H-resistant form, suggesting that co-exp

85 SP-B and SP-BDeltaC remained in cells in an endoglycosidase H-resistant form.

86 dified by N-linked glycosylation to a mature endoglycosidase H-resistant form.

87 ed medium, these chains were converted to an endoglycosidase H-resistant form.

88 t product being a relatively unstable 24-kDa endoglycosidase H-resistant glycoprotein.

89 pancies of numerous N-glycosylation sites by endoglycosidase H-resistant N-glycans originating from M

90 rporated into POMC predominantly on N-linked endoglycosidase H-resistant oligosaccharides.

91 alpha subunit, but not non-cleaved alpha, is endoglycosidase H-resistant.

92 is expressed on the cell surface as a 48-kDa endoglycosidase-H-resistant glycoprotein.

93 D deglycosylated with endoglycosidase F2 and endoglycosidase H retained ANP-binding activity and show

94 leavage site mutant I was N glycosylated and endoglycosidase H sensitive, indicating that ER transloc

95 cular chaperones BiP and GRP94, and remained endoglycosidase H sensitive, suggesting retention in the

96 emonstrated that the M(r) 85,000 species was endoglycosidase H sensitive, suggesting targeting of the

97 and gamma(c) chains associated with p12I are endoglycosidase-H sensitive, suggesting that their inter

98 cosylation sites and unknown numbers of both endoglycosidase H-sensitive and -resistant oligosacchari

99 Endoglycosidase H-sensitive class I molecules were detec

100 l cytoplasmic domain show that they exit the endoglycosidase H-sensitive compartment at a slower rate

101 x glycosylation of wild-type AQP2 but mainly endoglycosidase H-sensitive core glycosylation of AQP2-T

102 re endoglycosidase H-resistant form, and the endoglycosidase H-sensitive ER form has a half-life of 2

103 retained in the endoplasmic reticulum in an endoglycosidase H-sensitive form associated with the mol

104 rbohydrate chains of SCAP were mostly in the endoglycosidase H-sensitive form when cells were grown i

105 s of S and MHVR via coimmunoprecipitation of endoglycosidase H-sensitive forms of the two proteins.

106 within the endoplasmic reticulum as immature endoglycosidase H-sensitive forms.

107 ells, CPD is initially produced as a 170-kDa endoglycosidase H-sensitive glycoprotein.

108 crystals were composed of correctly folded, endoglycosidase H-sensitive IgG.

109 sed surface IgM coupled with accumulation of endoglycosidase H-sensitive IgM intracellularly, resembl

110 NAc2-PP-dolichol transferred by TbSTT3A, and endoglycosidase H-sensitive N-glycans originating from M

111 ocol; describes the presence of full-length, endoglycosidase H-sensitive NSP4 in plasma membrane cave

112 The enzyme contains endoglycosidase H-sensitive oligosaccharides that contai

113 d-type gE were synthesized as N-glycosylated endoglycosidase H-sensitive precursors with approximate

114 This Man(6)GlcNAc(2) is the endoglycosidase H-sensitive product of the Alg3p step.

115 peared mostly as a 70-kDa core-glycosylated, endoglycosidase H-sensitive, immature form bound to the

116 Endoglycosidase H sensitivity assays showed that N-linke

117 munofluorescence microscopy and constitutive endoglycosidase H sensitivity in metabolically labeled c

118 between the two cell lines as determined by endoglycosidase H sensitivity of newly sensitized P-glyc

119 dopsin as judged by mobility on SDS/PAGE and endoglycosidase H sensitivity.

120 al immature glycosylation, manifest by their endoglycosidase H sensitivity.

121 ome processed fertilin alpha is sensitive to endoglycosidase H, suggesting cleavage occurs prior to t

122 lted in some enzyme that lost sensitivity to endoglycosidase H, suggesting that the first disulfide b

123 TR-infected cells, gO remained sensitive to endoglycosidase H, suggesting that the protein was not e

124 We used the glycosidase endoglycosidase H to determine that this difference in m

125 tor N-butyldeoxynojirimycin and treated with endoglycosidase H to remove all but the terminal N-acety

126 We have used endoglycosidase H to remove the Asn-linked glycans from

127 d material to dense lysosomes was delayed in endoglycosidase H treated cells, but the rate of degrada

128 Pulse-chase experiments in combination with endoglycosidase H treatment determined that the mutant m

129 Endoglycosidase H treatment of microsomes containing nas

130 d be inhibited by d-mannose and abolished by endoglycosidase H treatment of THP, suggesting that the

131 Endoglycosidase H treatment of ts045VSVG-GFP confirmed t

132 Endoglycosidase H treatment resulted in the removal of o

133 In addition to transcription/translation, endoglycosidase H treatment was used to verify glycosyla

134 class I molecules exhibiting sensitivity to endoglycosidase H treatment.

135 toreceptors, P/rds was robustly sensitive to endoglycosidase H, which is consistent with its bypassin