ライフサイエンスコーパス: endolysin

1 ells by the enzymatic action of the released endolysin.

2 ly becomes permeabilized to the fully folded endolysin.

3 ng fusion to murein degradation by the phage endolysin.

4 es specificity of the catalytic domain of an endolysin.

5 lysin of Erwinia phage ERA103, is also a SAR endolysin.

6 lysis by bacteriophage encoding a secretory endolysin.

7 letely incompetent for lysis with the hybrid endolysin.

8 ntly of, but in conjunction with, holins and endolysins.

9 determines the recognition by bacteriophage endolysins.

10 d subsequent staphylolytic activity for some endolysins.

11 e two proteins for efficient host lysis: the endolysin, a muralytic enzyme, and the holin, a small me

12 igomers form membrane lesions, through which endolysin, a muralytic enzyme, escapes the cytoplasm to

13 Pinholins require secreted SAR endolysins, a pairing that may represent an intermediate

14 cheduled time after infection and allows the endolysin access to its substrate, resulting in host cel

15 uralytic enzymes that degrade the cell wall; endolysins accumulate in the cytosol fully folded during

16 ction cycle in which intracellular phage and endolysin accumulation can continue for hours.

17 Two proteins, an endolysin and a holin, are essential for host lysis by b

18 a lytic process requiring, at a minimum, an endolysin and a holin.

19 e that encodes an outer membrane lipoprotein endolysin and also spanin gene families that provide ins

20 tion site change the oligomeric state of the endolysin and reduce lytic activity.

21 an transglycosylase related to bacteriophage endolysins and acts as an autolysin in the stationary ph

22 rnative mode of treatment both bacteriophage endolysins and bacteriocins have been shown to possess a

23 membrane, leading to activation of secreted endolysins and murein degradation.

24 lish host lysis using a muralytic enzyme, or endolysin, and a holin, which permeabilizes the membrane

25 pinholin, a signal anchor and release (SAR) endolysin, and Rz and Rz1 equivalents.

26 k the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where th

27 PGHs of bacteriophage origin, called endolysins, are involved in bacterial lysis at the end o

28 ay have broad implications for the design of endolysins as therapeutic agents.

29 SAR endolysins become activated by various mechanisms upon r

30 Toyofuku et al. show that a prophage-encoded endolysin can generate holes in the cell wall through wh

31 This is the first report that a native endolysin can traverse epithelial membranes, thus substa

32 dulated by reconstitution of the full-length endolysin-CBD complex with free CBD.

33 Endolysin CD27L causes cell lysis of the pathogen Clostr

34 uring infection, the truncated bacteriophage endolysin CHAPK and the staphylococcal bacteriocin lysos

35 present a crystal structure of the activated endolysin CTP1L that targets Clostridium tyrobutyricum,

36 bited a holin-like function by promoting the endolysin-dependent lysis of an induced lambda lysogen t

37 as not due to the proteolytic release of the endolysin domain of the R-beta-Gal fusion protein.

38 hage T4 effects lysis by its holin T and its endolysin E.

39 4 effects host lysis with a holin, T, and an endolysin, E.

40 herefore, it is proposed that specificity of endolysins for specific bacilli is achieved by selective

41 has implications for the design of effective endolysins for the treatment of bacterial infections.

42 Leu 98 to a Trp residue which is found in an endolysin from a bacteriophage of Listeria monocytogenes

43 unprecedented size (>300 nm), releasing the endolysin from the cytoplasm, resulting in lysis within

44 enough to allow release of prefolded active endolysin from the cytoplasm, which results in destructi

45 The other, AtlA, is similar to endolysins from bacteriophages and is not similar to any

46 Because endolysin function requires the formation of mum-scale h

47 coexpressed with Rz1, despite the absence of endolysin function.

48 When the CTP1L endolysin gene is introduced into the commensal bacteriu

49 n internal translation start site within the endolysin gene.

50 , three new putative intron-containing phage endolysin genes were identified in public data sets for

51 sis cassette, (ii) constitutively expressing endolysin genes while restricting holin genes, and (iii)

52 eriophage lambda requires only the holin and endolysin genes, but not the Rz and Rz1 genes, of the ly

53 tein (gp17), the phage integrase (gp29), the endolysin (gp31), the phage repressor (gp47), and six pr

54 R, the endolysin, has muralytic activity.

55 Endolysins have acquired stringent substrate specificiti

56 the holin and antiholin, and R, encoding the endolysin, have been intensively studied, the products o

57 ow that the expression of a prophage-encoded endolysin in a sub-population of cells generates holes i

58 havior, causing bacterial lysis by releasing endolysin in an oligomerization-dependent manner.

59 e membrane were nonpermissive for the hybrid endolysin, indicating that these premature lesions const

60 After peptidoglycan digestion with phage endolysin, InlA-MH(6)-Cws was purified by affinity chrom

61 uncated T holin functional in lysis with the endolysin is completely incompetent for lysis with the h

62 Thus, the exported P1 endolysin is kept inactive by three levels of control-to

63 Endolysin is the term for muralytic enzymes that degrade

64 We have characterized two endolysins, Lc-Lys and Lc-Lys-2, identified in prophages

65 Putative lysis genes encoding endolysin (Lys) and holin proteins were identified from

66 PlyC, a bacteriophage-encoded endolysin, lyses Streptococcus pyogenes (Spy) on contact

67 doid phage 21 has the prototype pinholin-SAR endolysin lysis system, which is widely distributed amon

68 In contrast, in pinholin-SAR endolysin lysis, the cell began to shorten and thicken u

69 In its inactive form, the prototype SAR endolysin, Lyz(P1), of coliphage P1, has an active-site

70 Our results support the idea that endolysins may be important for MV formation in bacteria

71 ults are discussed in terms of a model where endolysin-mediated degradation of the cell wall is a pre

72 The Lyz endolysin of bacteriophage P1 was found to cause lysis o

73 Here, we report that Lyz(103), the endolysin of Erwinia phage ERA103, is also a SAR endolys

74 e peptidoglycan degrading transglycosylases (endolysins) of bacteriophages lambda and P2, suggesting

75 We further show that a related endolysin (Ply21) from the B. cereus phage, TP21, shows

76 incorporation of the listeria bacteriophage endolysin Ply500: covalent attachment onto FDA approved

77 binding the cell wall binding domains of the endolysins PlyL and PlyG were determined by surface plas

78 ctional analysis of the lambda prophage Ba02 endolysin (PlyL) encoded by the Bacillus anthracis genom

79 aused by these pores activates the muralytic endolysin, R(21), leading immediately to peptidoglycan d

80 her lysostaphin (bacteriocin) or LysK (phage endolysin) resulted in a approximately 5x increase in st

81 age 21 uses a pinholin-signal anchor release endolysin strategy to effect temporally regulated host l

82 e protein sequences of related bacteriophage endolysins suggests that the presence of an N-terminal s

83 ea and appears to be distinct from the holin/endolysin system described for DNA bacteriophages.

84 Bacteriophage lambda uses a holin-endolysin system for host cell lysis.

85 hages with either the lambda canonical holin-endolysin system or the phage 21 pinholin-signal anchor

86 hage 21 pinholin-signal anchor release (SAR) endolysin system.

87 rmediate in the evolution of canonical holin-endolysin systems.

88 igomerization mechanism applies to the CD27L endolysin that targets Clostridium difficile and the CS7

89 targets Clostridium difficile and the CS74L endolysin that targets Clostridium sporogenes.

90 yz(P1) and Lyz(103) define subclasses of SAR endolysins that differ in the nature of their inhibitory

91 acteria for the primary purpose of releasing endolysins that hydrolyze the cell wall and induce cell

92 Moreover, on coexpression of holin and endolysin, the degradation of the cell wall leads to sph

93 a holin that mediates the movement of the T4 endolysin though the inner cell membrane to its target,

94 This permeabilization enables the R endolysin to attack the cell wall, after which cell lysi

95 ethal membrane lesion which allows the phage endolysin to attack the peptidoglycan.

96 hat AtlA can functionally replace the lambda endolysin to lyse Escherichia coli.

97 y and sufficient not only for export of this endolysin to the membrane but also for its release into

98 We show that truncation of the endolysin to the N-terminal domain, CD27L1-179, gave an

99 a normal signal-arrest domain to direct the endolysin to the periplasm in membrane-tethered form and

100 specific release of pre-folded, fully active endolysins to the periplasm.

101 ctive cycle, allow bacteriolytic enzymes, or endolysins, to escape to the periplasm and to attack the

102 ccess of phage-encoded muralytic enzymes, or endolysins, to the cell wall by the sudden formation of

103 nding domains of the streptococcal LambdaSa2 endolysin were replaced by staphylococcal SH3b domains f

104 Bacteriophages express endolysins which are the enzymes that hydrolyze peptidog

105 e hosts has been shown to require holins and endolysins, which attack the cytoplasmic membrane and pe

106 Bacteriophages produce endolysins, which lyse the bacterial host cell to releas

107 In contrast to canonical phage endolysins, which require holin-mediated disruption of t

108 A C-terminal fusion of the R endolysin with full-length beta-galactosidase (beta-Gal)