ライフサイエンスコーパス: endonuclease I

1 iday junctions by T4 endonuclease VII and T7 endonuclease I.

2 or 10-nucleotide flap DNA substrate by Flap Endonuclease I.

3 hanism of the amyloidogenic conversion of T7 endonuclease I.

4 volved in this process is structure-specific endonuclease-I.

5 However, the Mms4-Mus81 endonuclease is 25 times more active on branched duplex

6 structures, suggesting that deoxyinosine 3'-endonuclease is a bacterial functional homologue of huma

7 The tRNA splicing endonuclease is a highly evolutionarily conserved protei

8 The PvuII restriction endonuclease is a homodimer that recognizes and cleaves

9 Apurinic/apyrimidinic endonuclease is a key enzyme in the process of base exci

10 The FokI restriction endonuclease is a monomeric protein that recognizes an a

11 The primary target of SgrAI restriction endonuclease is a multiple sequence of the form 5'-CPu/C

12 side following incision at its 5' side by AP endonuclease is a prerequisite to completion of these re

13 The SfiI endonuclease is a prototype for DNA looping.

14 The SfiI restriction endonuclease is a tetramer in which two subunits form a

15 dual recognition sites 4-6 bp long, the SfiI endonuclease is a tetrameric protein that binds to two c

16 The SfiI endonuclease is a tetrameric protein with two DNA-bindin

17 Bacterial RNA-directed Cas9 endonuclease is a versatile tool for site-specific genom

18 p, a member of the RNase III family of dsRNA endonucleases, is a key component of the Saccharomyces c

19 Here we show that T7 endonuclease I, a naturally domain-swapped dimeric prote

20 When the T7 gene 3-encoded DNA debranching endonuclease is absent during in vitro T7 DNA concatemer

21 Internal cleavage by this endonuclease is accelerated by the presence of a monopho

22 owever, the mechanism by which the Mlh1-Mlh3 endonuclease is activated is unknown.

23 tion and chemical crosslinking indicate that endonuclease I also exists in free solution as a dimer t

24 Crenarchaeal XPF, a 3'-flap endonuclease, is also stimulated by PCNA in vitro.

25 The influenza endonuclease is an essential subdomain of the viral RNA

26 Serratia endonuclease is an important member of a class of magnes

27 The EcoRI endonuclease is an important recombinant DNA tool and a

28 intriguing hypothesis in which the splicing endonuclease is an intermediate in the transition from t

29 This proved that the endonuclease is an intrinsic activity of baculovirus AN.

30 Dicer, an RNase III endonuclease, is an essential component of the microRNA

31 ta show that the form of the complex between endonuclease I and a DNA junction depends on the core of

32 e have constructed a model of the complex of endonuclease I and a DNA junction.

33 complex with a junction-resolving enzyme, T7 endonuclease I, and indeed, one intermediate forms a sta

34 rmined the specificity profile of the homing endonuclease I-AniI and compared it to the conservation

35 dentified homologues of the LAGLIDADG homing endonuclease I-AniI and their putative target insertion

36 The monomeric homing endonuclease I-AniI cleaves with high sequence specifici

37 dditional depletion of apurinic/apyrimidinic endonuclease I (APE1) confers hypersensitivity above tha

38 myloid-like fibrils in which molecules of T7 endonuclease I are linked by intermolecular disulfide bo

39 We find that ErrASE and T7 Endonuclease I are the most effective at decreasing aver

40 eavage activities observed for the native T7 endonuclease I (as distinct from the resolution activity

41 cally evaluated by low throughput assays (T7 endonuclease I assay, target amplification followed by h

42 he continuous strands of a junction bound by endonuclease I, at sites close to (but not identical wit

43 This endonuclease is believed to function in the initial step

44 C1-XPF heterodimer, a structure-specific DNA endonuclease, is best known for its function in the nucl

45 n (H144), consisting of Lac repressor and T7 endonuclease I, binds at the lac operator and cleaves re

46 The endonuclease is blocked by ATP and is thus experimentall

47 Here, we describe a GIY-YIG family homing endonuclease, I-BmoI, that possesses an unusual recognit

48 ate we demonstrate that an active subunit of endonuclease I can act as a junction-specific nuclease i

49 hat the HsdR subunit of a type I restriction endonuclease is capable of independent enzyme activity,

50 Using mass spectrometry we have shown that endonuclease I catalyzes the breakage of the P-O3' bond,

51 RNA interference of structure-specific endonuclease-I caused persistence of a single ribonucleo

52 e III family of double-stranded RNA-specific endonucleases is characterized by the presence of a high

53 In mice, this endonuclease is coded by the Apex gene, disruption of wh

54 The discussion of type II restriction endonucleases is comprised of a description of the gener

55 Finally, the evolution of homing endonucleases is considered, both at the structure-funct

56 cture of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been de

57 Both heatshock-inducible endonuclease I-CreI expression and X-ray irradiation can

58 es in which the homodimeric LAGLIDADG homing endonuclease I-CreI is altered at individual side-chains

59 etic approach in analyzing individual homing endonuclease I-CreI protein/DNA contacts, and describe h

60 The homing endonuclease I-CreI recognizes and cleaves a particular

61 We used the endonuclease I-CreI to generate acentric chromosomes in

62 The homing endonuclease, I-CreI, was originally isolated from the c

63 rget sites of the canonical LAGLIDADG homing endonucleases I-CreI and I-MsoI.

64 ence of the amyloidogenic conformation of T7 endonuclease I depends sensitively on solution condition

65 as also supported by their sensitivity to T7 endonuclease I digestion.

66 ural variant of the CEL I family of mismatch endonucleases is discussed.

67 t AAV, the DNA sequence cleaved by the viral endonuclease is distinct from all other described seroty

68 Ty1ADE2 elements also carried a site for the endonuclease I-DmoI, which we demonstrate is not present

69 fic endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400

70 te, although some restoration of activity in endonuclease I E65D was observed in the presence of Mn2+

71 The I-PpoI endonuclease is encoded by a group I intron found in the

72 The N-terminal region of EcoRI endonuclease is essential for cleavage yet is invisible

73 unit of ERCC1-XPF nucleotide excision repair endonuclease, is essential for meiosis and important for

74 raction before the engineering of new homing endonucleases is essential for further enzyme modificati

75 Digestion of Holliday junctions with T7 endonuclease I establishes that the junction is not trap

76 restricting phage lambda infection when the endonuclease is expressed in E. coli.

77 The yeast HO endonuclease is expressed in late G1 in haploid mother c

78 of one of the DNA replication factors, flap endonuclease I (FEN1), in regulating telomerase activity

79 gmentation by a methyl-sensitive restriction endonuclease is followed by size fractionation and hybri

80 Previously we showed that T7 endonuclease I forms amyloid-like fibrils.

81 We show that T7 endonuclease I forms fibrils at pH 6.8, but not at pH 6.

82 lting covalently linked fibrils show that T7 endonuclease I forms fibrils by a runaway domain swap.

83 igestion of linear DNA by type I restriction endonucleases is generally activated following the head-

84 The cellular concentration of this endonuclease is governed by a feedback mechanism in whic

85 The crystal structure of endonuclease I has been solved both as free protein and

86 The introns encode the DNA endonucleases I-HmuI and I-HmuII, respectively, which be

87 We have studied the structure-specific endonuclease-I homolog from Trypanosoma brucei, showing

88 The Mus81-Eme1 endonuclease is implicated in the efficient rescue of br

89 homology to the XPF subunit of the ERCC1-XPF endonuclease, is important for replicational stress tole

90 ordinator of multiple DNA structure-specific endonucleases, is important for several DNA repair pathw

91 re of the junction-resolving enzyme phage T7 endonuclease I in complex with a synthetic four-way DNA

92 nterica serovar Paratyphi A, the restriction endonuclease is inactive, apparently due to a mutation i

93 In contrast to the exonuclease, the endonuclease is inhibited completely by 32 protein bindi

94 The human Mus81-Eme1 endonuclease is involved in recombination repair, but th

95 The ERCC1-XPF endonuclease is involved in this unhooking step and in t

96 Endonuclease I is a 149 amino acid protein of bacterioph

97 T7 endonuclease I is a dimeric nuclease that is selective f

98 Endonuclease I is a DNA junction-selective resolving enz

99 Endonuclease I is a junction-resolving enzyme encoded by

100 T7 endonuclease I is a nuclease that is selective for the s

101 Phage-encoded resolvase T7 endonuclease I is a structure-specific endonuclease.

102 Endonuclease I is active in the presence of magnesium, m

103 The junction-resolving enzyme endonuclease I is selective for the structure of the DNA

104 obtain hydroxyl radical footprinting data on endonuclease I-junction complexes that are not complicat

105 age specificity of the intron-encoded homing endonuclease I-MsoI using a physically realistic atomic-

106 f-target analysis was performed using the T7 endonuclease I mutation detection assay and Sanger seque

107 The gene encoding the vsr endonuclease is next to the gene specifying the E. coli

108 r the cleavage reaction, the identity of the endonuclease is not known.

109 Endonuclease I of bacteriophage T7 is a DNA junction-res

110 Endonuclease I of phage T7 is a member of the nuclease s

111 iday junctions, whereas the Mus81-associated endonuclease is one order of magnitude more active upon

112 However, the endonuclease is poorly expressed in the cytosolic fracti

113 s of DSBs induced by either radiation or the endonuclease I-PpoI in human somatic cells.

114 The homing endonuclease I-PpoI severely bends its DNA target, resul

115 aracterize variants of the eukaryotic homing endonuclease I-PpoI that were able to bind a mutant, cle

116 comparison to one other nuclease, the homing endonuclease I-PpoI, that has recently been shown, in sp

117 by exploiting the specificity of the homing endonuclease I-PpoI, which is able to selectively cleave

118 of direct protein transduction of the homing endonuclease, I-PpoI, into human cells to generate site-

119 zed cleavage sites for the eukaryotic homing endonucleases I-PpoI and I-CreI were constructed, and si

120 tially corrects C/G transversions whereas T7 Endonuclease I preferentially corrects A/T transversions

121 Remarkably, no homolog of this key endonuclease is present in many bacterial species, such

122 rk forms when the gene 3-encoded debranching endonuclease is present.

123 nism for catalysis of DNA cleavage by I-PpoI endonuclease is proposed and tested by creating six vari

124 The yUtp24/hUTP24 PIN endonuclease is proposed to cleave at sites A1/1 and A2/

125 inding and cleavage by the SgrAI restriction endonuclease is proposed.

126 RNase III, a double-stranded RNA-specific endonuclease, is proposed to be one of Escherichia coli'

127 and DNA repair enzyme apurinic/apyrimidinic endonuclease I protect smooth muscle cells against oxida

128 in Saccharomyces cerevisiae, encoding the HO endonuclease, is regulated by two distinct PUF proteins,

129 property of the exonuclease III family of AP endonucleases is remarkably conserved from Archaea to hu

130 Our results also suggest that the Ercc1/Xpf endonuclease is required for efficient removal of non-ho

131 ERCC1-XPF endonuclease is required for nucleotide excision repair

132 The Fan1 endonuclease is required for repair of DNA interstrand c

133 The nucleolytic activity of MUS81 endonuclease is required for replication fork restart un

134 strand crosslink (ICL) repair, the XPF-ERCC1 endonuclease is required for the incisions that release,

135 Further, the Apn2 endonuclease is required for the viability of smc6 mutan

136 The Rad1/Rad10 endonuclease is required to trim intermediates formed du

137 The endonuclease is resistant to RNase A inhibitors, sensiti

138 Digestion of the dHJ substrate by T7 endonuclease I resolves the substrate into crossover and

139 The SP82-encoded endonuclease is responsible for exclusion of the SPO1 in

140 In yeast, the tRNA splicing endonuclease is responsible for identification and cleav

141 The RNA splicing endonuclease is responsible for recognition and excision

142 tails after treatment with the rare-cutting endonuclease I-SCE:I.

143 initiated by DSBs induced by a rare cutting endonuclease (I-SceI) into one of two direct DNA repeats

144 of two tandem noncohesive DSBs generated by endonuclease I-SceI and the 3' nonprocessive exonuclease

145 rate containing a recognition site for yeast endonuclease I-SceI embedded within a functional tk gene

146 rate containing a recognition site for yeast endonuclease I-SceI embedded within a functional tk gene

147 A genomic DSB was induced by introducing endonuclease I-SceI into cells containing a stably integ

148 romosomal double-strand break created by the endonuclease I-SceI or multiple breaks created by treatm

149 system is based on the stable integration of endonuclease I-SceI recognition sites flanked by bacteri

150 This method uses the mitochondrial endonuclease I-SceI that recognizes and cuts an 18-bp re

151 hen electroporated with a plasmid expressing endonuclease I-SceI to induce a DSB, and clones that had

152 The same result was obtained when the homing endonuclease I-SceI was used to initiate HDR at the targ

153 that combines the specificity of the homing endonuclease I-SceI with the type IIS cleavage pattern o

154 ere electroporated with a plasmid expressing endonuclease I-SceI, and clones that had lost tk functio

155 ining a cleavage site for the intron-encoded endonuclease I-SceI, which is not otherwise found in the

156 le strand break introduced by a rare cutting endonuclease I-SceI.

157 pted by the 18-bp recognition site for yeast endonuclease I-SceI.

158 ne, or no genomic sites for the rare-cutting endonuclease I-SceI.

159 de containing the 18-bp recognition site for endonuclease I-SceI.

160 Bacteriophage T7 endonuclease I selectively binds and cleaves DNA four-wa

161 ined metastable conformational states within endonuclease I, showing how these states can be influenc

162 Unlike most ribonucleases, this viral endonuclease is shown to catalyze the hydrolysis of the

163 L1 endonuclease is specific for the unusual DNA structural

164 The homing endonuclease I-Ssp6803I causes the insertion of a group

165 inding and cleavage by the PD-(D/E)XK homing endonuclease I-Ssp6803I.

166 his intronic ORF encodes a double-strand DNA endonuclease, I-Ssp6803I.

167 that the N-terminal two-thirds of the I-CreI endonuclease is sufficient for homing site recognition a

168 ed into cells, suggesting that any apoptotic endonuclease is sufficient to induce gamma-H2AX formatio

169 ion between protein and enzyme activity, the endonuclease is tentatively identified as a approximatel

170 After the td intron endonuclease I-TevI cleaves the intronless recipient 23

171 volution experiments with the GIY-YIG homing endonuclease I-TevI that targets the thymidylate synthas

172 td intron is initiated by the intron-encoded endonuclease I-TevI, which cleaves the intronless allele

173 ce of the phage T4 td group I intron and its endonuclease, I-TevI, for which the distance separating

174 The phage T4 td intron endonuclease, I-TevI, is responsible for this infidelity

175 We have also constructed a mutant of endonuclease I that lacks nine amino acids (six of which

176 The CRISPR-Cas-derived RNA-guided Cas9 endonuclease is the key element of an emerging promising

177 The nuclear localization of this endonuclease is the unique feature that distinguishes it

178 EcoRV endonuclease is thus not a "new paradigm" for site-speci

179 nts to analyze induced fit in the binding of endonuclease I to a DNA four-way junction.

180 ude that the function of the retrotransposon endonucleases is to define and cleave target site DNA.

181 the Hol75 DNA by T4 endonuclease VII and T7 endonuclease I, two enzymes known to cleave such junctio

182 Intron 2 encodes a DNA endonuclease, I-TwoI, with similarity to homing endonucl

183 eature between an intronic and free-standing endonuclease is unprecedented.

184 I-TevI, the T4 td intron-encoded GIY-YIG endonuclease, is very similar to I-BmoI, but each endonu

185 orrectly folded catalytic domain (SCD) of T7 endonuclease I was produced by means of a trans-splicing

186 phosphorylation sites, recombinant human AP endonuclease is weakly phosphorylated in vitro (4% at be

187 cture of the DNA bound in the active site of endonuclease I, where the proS oxygen atom is coordinate

188 in other tissues, suggesting that the repair endonuclease is widely utilized for repair of DNA damage

189 We have solved the crystal structure of endonuclease I with a wild-type active site.