ライフサイエンスコーパス: enoyl-CoA hydratase

1 larly important to the catalytic activity of enoyl-CoA hydratase.

2 Glu139 are required for the high kcat of the enoyl-CoA hydratase.

3 i was identified as the catalytic residue of enoyl-CoA hydratase.

4 A ligase, and RpfF shows some relatedness to enoyl CoA hydratases.

5 ., E269 in aldehyde dehydrogenase-1; D204 in enoyl CoA hydratase-1), as well as residues of unknown f

6 thors show that exceeding nutrients suppress Enoyl-CoA hydratase-1 (ECHS1) activity by inducing its a

7 Herein, we report that enoyl-CoA hydratase-1 (ECHS1), the enzyme involved in th

8 Aconitase-2 and enoyl-CoA-hydratase-1 expression levels were decreased i

9 Depletion of aconitase-2 and enoyl-CoA-hydratase-1 resulted in the inhibition of the

10 selective depletion of both aconitase-2 and enoyl-CoA-hydratase-1.

11 hat may contribute to renal function include enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (EHH

12 l 3-oxoacyl-CoA thiolase (THIO), peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (HD)

13 hree enzymes: fatty acyl-CoA oxidase (ACOX), enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (HD)

14 A weak enoyl-CoA hydratase activity was detected for both DpgB

15 lutaryl-CoA dehydrogenase also has intrinsic enoyl-CoA hydratase activity, a property of other member

16 ectra have been obtained for HD-CoA bound to enoyl-CoA hydratase, an enzyme system that has also prev

17 y and show another stable complex, PaaFG, an enoyl-CoA hydratase and enoyl-Coa isomerase, both belong

18 hexadienoyl-CoA (HD-CoA), bound to wild-type enoyl-CoA hydratase and G141P, a mutant in which a hydro

19 tyl-CoA when bound as an enolate to MCAD and enoyl-CoA hydratase and is used to rationalize the obser

20 ur separate reactions, two of which (2-trans enoyl-CoA hydratase and L-3-hydroxyacyl-CoA dehydrogenas

21 o contains the active site of long-chain 2,3-enoyl-CoA hydratase and long-chain 3-ketoacyl-CoA thiola

22 lytic properties of 3-ketoacyl-CoA thiolase, enoyl-CoA hydratase, and delta 3-cis-delta 2-trans-enoyl

23 Enoyl-CoA hydratase catalyzes the hydration of trans-2-c

24 Significantly, binding to enoyl-CoA hydratase causes the chemical shifts of the C1

25 th members of the crotonase family including enoyl-CoA hydratase (crotonase) and methylmalonyl-CoA de

26 Members of the enoyl-CoA hydratase (crotonase) superfamily catalyze dif

27 Gene deletion analyses confirmed that the enoyl-CoA hydratase/dehydrogenase Fox2p, the putative 3-

28 termined the crystal structure of the enzyme enoyl-CoA hydratase (ECH) from rat liver with the bound

29 enecyclopropyl)glycine, against bovine liver enoyl-CoA hydratase (ECH) were characterized.

30 TFTH-CoA can be directly bioactivated by the enoyl-CoA hydratase (ECH) with the release of 1,2-dichlo

31 ium (PDB ID 3q1t) has been reported to be an enoyl-CoA hydratase (ECH), but SALSA analysis shows a po

32 ve site-directed inactivator of bovine liver enoyl-CoA hydratase (ECH).

33 ditions for crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and

34 ditions for crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and

35 noyl-coenzyme A (HD-CoA) bound to the enzyme enoyl-CoA hydratase has been determined using transferre

36 sidues (E164 and E144) in the active site of enoyl-CoA hydratase has been probed by site-directed mut

37 e promoter of the PPARalpha target genes rat enoyl-CoA hydratase (HD) and peroxisomal fatty acyl-CoA

38 These data suggest that enoyl-CoA hydratase is an important enzyme in the bioact

39 rases but showed significant homology to the enoyl-CoA hydratase/isomerase enzyme family.

40 athways associated with the members of the 2-enoyl-CoA hydratase/isomerase enzyme superfamily are com

41 esis, the active site of one member of the 2-enoyl-CoA hydratase/isomerase family, 4-chlorobenzoyl-Co

42 se/reductase family and that IBR10 resembles enoyl-CoA hydratases/isomerases.

43 oxidation at the level of the second enzyme, enoyl-CoA hydratase/L-3-hydroxyacyl-CoA dehydrogenase (L

44 In this study, the function of enoyl-CoA hydratase/L-3-hydroxyacyl-CoA dehydrogenase (L

45 ex enhanced transcription from a peroxisomal enoyl-CoA hydratase/l-3-hydroxyacyl-CoA dehydrogenase bi

46 especially of CBP and TRAP150, to the mouse enoyl-CoA hydratase/l-3-hydroxyacyl-CoA dehydrogenase ge

47 urated substrates bind to the active site of enoyl-CoA hydratase, large spectral changes can be obser

48 of the component enzymes of TOC, long-chain enoyl-CoA hydratase, long-chain 3-hydroxyacyl-CoA dehydr

49 somal beta-oxidation mutants showed that the enoyl CoA-hydratase MAOC-1 serves an important role in a

50 In contrast, growth of a putative enoyl-CoA hydratase mutant (DeltaechA) was abolished on

51 eding experiments show that the MFP2 2-trans enoyl-CoA hydratase only exhibits activity against long

52 of the buffer effect on the mechanism of the enoyl-CoA hydratase reaction is discussed.

53 n genes trxA (Rv1470), trxB (Rv1471), and an enoyl-coA hydratase (Rv1472), indicating a possible role

54 ed enzymes, the acyl-CoA ligase SidI and the enoyl-CoA hydratase SidH, linking biosynthesis of mevalo

55 uence homology validates the modeling of the enoyl-CoA hydratase structure with the 4-(chlorobenzoyl)

56 G141 is part of a consensus sequence in the enoyl-CoA hydratase superfamily, the results presented h

57 e encodes seven paralogues of the crotonase (enoyl CoA hydratase) superfamily.

58 coupled with the strong sequence homology to enoyl-CoA hydratase support the intramolecular suprafaci

59 wo conserved glutamate residues of rat liver enoyl-CoA hydratase to which Glu119 and Glu139 of the la

60 hat is catalytically essential in homologous enoyl-CoA hydratases was also essential in CHY1.

61 -fold decrease, respectively, in the kcat of enoyl-CoA hydratase without a significant change in the