ライフサイエンスコーパス: enterobactin

1 duct that is vital for the final assembly of enterobactin.

2 ransporter IroC exports both salmochelin and enterobactin.

3 rolysis and cyclization of the iron chelator enterobactin.

4 suggesting selection against a dependence on enterobactin.

5 ormally function in the adsorption of ferric enterobactin.

6 the transport of the natural ligand, ferric enterobactin.

7 al features and solution chemistry of ferric enterobactin.

8 tor by a mechanism requiring the siderophore enterobactin.

9 al deletion of entC, preventing synthesis of enterobactin.

10 modest expression levels in cultures lacking enterobactin.

11 cteria upon supplementation of cultures with enterobactin.

12 indicates that both VctA and IrgA transport enterobactin.

13 . cholerae transports, but does not make, is enterobactin.

14 a strain defective in both genes did not use enterobactin.

15 ring its transport of the siderophore ferric enterobactin.

16 iderophores yersiniabactin, salmochelin, and enterobactin.

17 r the siderophores triacetylfusarinine C and enterobactin.

18 the biosynthesis of the catechol siderophore enterobactin.

19 ) incapable of synthesizing the siderophore, enterobactin.

20 ctive in the biosynthesis of the siderophore enterobactin.

21 ed by a regulator that requires induction by enterobactin.

22 ctin, which it synthesizes and secretes, and enterobactin.

23 r to 2,3-DHBA, the iron-binding component of enterobactin.

24 cific to the biosynthesis of the siderophore enterobactin.

25 , DeltaaebF V. harveyi, do not produce amphi-enterobactins.

26 Vmax for [59Fe]-enterobactin (0.15 pMol per 10(9) cells per minute) was

27 educing the affinity between FepA and ferric enterobactin 100- and 10-fold respectively.

28 In iron-depleted cultures containing enterobactin, a Bordetella bfeR mutant exhibited markedl

29 Enterobactin, a catecholate siderophore, was not a subst

30 The siderophore molecule enterobactin, a cyclic trimeric lactone of N-(2,3-dihydr

31 inearized and monoglycosylated derivative of enterobactin, a nonribosomal peptide and iron scavenger

32 ositive bacteria, is structurally similar to enterobactin, a well known siderophore isolated from Gra

33 ved in CfrA, plays a critical role in ferric enterobactin acquisition in C. jejuni.

34 Cells unable to import enterobactin across the outer membrane grow normally, wh

35 bule, and measurements of the rate of ferric enterobactin adsorption to fluoresceinated FepA mutant p

36 Three are linked to genes for enterobactin, aerobactin, and yersiniabactin.

37 onse to the structurally similar siderophore enterobactin, although genetic analyses indicate that th

38 C-glycosylated and linearized derivative of enterobactin, an iron scavenger (siderophore) and produc

39 By using a series of isosteric enterobactin analogues, the contribution of electrostati

40 n iron source or to utilize the siderophores enterobactin and aerobactin, indicating that transport o

41 that present in natural siderophores such as enterobactin and azotochelin.

42 ipate in the binding and transport of ferric enterobactin and colicins B and D.

43 In vitro, a mixture of enterobactin and copper was toxic for E. coli cells, but

44 The structures of ferric enterobactin and ferric enantioenterobactin obtained in

45 d FhuA, which recognize and transport ferric enterobactin and ferrichrome, respectively.

46 terns of the Bordetella pertussis alcaligin, enterobactin and haem iron acquisition systems were exam

47 nown outer membrane receptors for alcaligin, enterobactin and haem, supporting the hypothesis that B.

48 amine receptors could serve as receptors for enterobactin and its degradation product 2,3-dihydroxybe

49 secretes two catecholate-type siderophores, enterobactin and its glucosylated derivative salmochelin

50 functional homolog of FepA that binds ferric enterobactin and may be part of a system responsible for

51 of tryptophan, folate, and the siderophores enterobactin and mycobactin, respectively.

52 iabactin positive (Ent(+) Ybt(+)) (17%), and enterobactin and salmochelin (glycosylated Ent) positive

53 the genes required to produce and transport enterobactin and salmochelin across the outer membrane r

54 ferric iron transporter and the siderophores enterobactin and salmochelin are required by Salmonella

55 take or the ferric iron binding siderophores enterobactin and salmochelin are required for persistent

56 roB (salmochelin deficient), hvKP1DeltaentB (enterobactin and salmochelin deficient), hvKP1Deltairp2

57 hores, only the catecholate xenosiderophores enterobactin and salmochelin promoted growth of gonococc

58 of gonococcal strain FA19 in the presence of enterobactin and salmochelin.

59 perfamily pump EntS is the major exporter of enterobactin and the ABC transporter IroC exports both s

60 the mutant proteins to interact with ferric enterobactin and the protein toxins colicins B and D.

61 cular dichroism properties of bacillibactin, enterobactin and the synthetic analogs d-enterobactin, S

62 loned DNA regained the ability to synthesize enterobactin and to grow in low-iron medium.

63 ydroxybenzoate and anthranilate, involved in enterobactin and tryptophan biosynthesis, respectively,

64 em that functions in the utilization of both enterobactin and vibriobactin (VCA0227-0230).

65 ified: enterobactin positive (Ent(+)) (81%), enterobactin and yersiniabactin positive (Ent(+) Ybt(+))

66 the synthetic, left-handed isomer of natural enterobactin, and ferric TRENCAM, which substitutes a te

67 e design, synthesis, and characterization of enterobactin-antibiotic conjugates, hereafter Ent-Amp/Am

68 Bacillibactin and enterobactin are hexadentate catecholate siderophores pr

69 an, tyrosine, and phenylalanine, which, like enterobactin, are synthesized from the precursor chorism

70 FA1090 to utilize the xenosiderophore ferric enterobactin as an iron source.

71 acultative pathogenic Alistipes spp. utilize enterobactin as iron source, bloom in Lcn2(-/-)/Il10(-/-

72 N. gonorrhoeae FA1090 utilized ferric enterobactin as the sole iron source when supplied with

73 utants exhibited impaired growth with ferric enterobactin as the sole source of iron, demonstrating t

74 i for iron uptake, 2,3-dihydroxybenzoate and enterobactin, as well as 3-hydroxyanthranilate, an iron

75 e sole iron source when supplied with ferric enterobactin at approximately 10 microM, but growth stim

76 siderophore in different environments, with enterobactin being more important for growth in vitro un

77 hat residues previously implicated in ferric enterobactin binding by FepA were partially conserved in

78 Ferric enterobactin binding to FepA did not prevent modificatio

79 utilization without causing a loss of ferric enterobactin binding.

80 explored, since at this site siderocalin, an enterobactin-binding mammalian gene product, is expresse

81 the interactions of these complexes with two enterobactin-binding proteins, which illuminate the infl

82 enterobactin biosynthesis, but not of other enterobactin biosynthesis genes, suppressed the mutant p

83 3-dihydroxybenzoate, the intermediate in the enterobactin biosynthesis pathway, and providing 2,3-dih

84 ich functions in the first dedicated step of enterobactin biosynthesis, but not of other enterobactin

85 , which catalyzes 2,3-DHBA production during enterobactin biosynthesis.

86 of VibBArCP for three catalytic partners in enterobactin biosynthesis.

87 ated that the defect was in an early step in enterobactin biosynthesis.

88 coli enterobactin gene cluster, in which the enterobactin biosynthetic and transport genes lie adjace

89 The amphi-enterobactin biosynthetic machinery was heterologously e

90 stimulated the growth of an Escherichia coli enterobactin biosynthetic mutant in low iron medium, and

91 umermycin A1, yersiniabactin, pyochelin, and enterobactin biosynthetic pathways as proof of principle

92 l specific activity of 15,500 pmol of ferric enterobactin bound/mg.

93 if any, enterobactin, but elevated levels of enterobactin breakdown products 2,3- dihydroxybenzoylser

94 e for the macrocyclic lactone ring of ferric enterobactin but maintains an unsubstituted catecholate

95 system, FepBDGC, allowed the utilization of enterobactin but not vibriobactin.

96 that the P43 mutant secretes little, if any, enterobactin, but elevated levels of enterobactin breakd

97 MceC and MceD derivatize enterobactin by C-glycosylation at the C5 position of a

98 n that is essential for the uptake of ferric enterobactin by Escherichia coli.

99 so compared the binding and uptake of ferric enterobactin by homologs of FepA from Bordetella bronchi

100 whereas TonB2 is required for utilization of enterobactin by V. cholerae.

101 lecules are key precursors to a family of 10 enterobactin-cargo conjugates presented in this work, wh

102 . coli for the uptake and utilization of the enterobactin-cargo conjugates, and growth promotion was

103 ved in the synthesis of the iron siderophore enterobactin, catalyzes the adenylation of 2,3-dihydroxy

104 induce transcription of the Bordetella bfeA enterobactin catechol xenosiderophore receptor gene, nei

105 n siderophore clusters, the Escherichia coli enterobactin cluster and the Vibrio cholera vibriobactin

106 chia coli outer membrane receptor for ferric enterobactin, colicin D and colicin B.

107 iron-depleted conditions in the presence of enterobactin, compared to modest expression levels in cu

108 e structure of the neutral protonated ferric enterobactin complex [Fe(III)(H(3)Ent)](0) has been the

109 its discovery over 40 years ago, the ferric enterobactin complex has eluded crystallographic structu

110 teria, and the crystal structure of a ferric enterobactin complex of a protein identified as an antib

111 a route for the chemoenzymatic synthesis of enterobactin conjugates with peptide linkages.

112 In contrast to enterobactin, corynebactin assumes a Lambda configuratio

113 l stability of the three ferric complexes of enterobactin, corynebactin, and the hybrid has been inve

114 an reverse this relationship, instead making enterobactin critical for overcoming SCN-mediated growth

115 A series of growth recovery assays employing enterobactin-deficient E. coli ATCC 33475 (ent-) reveale

116 le of complementing the growth defect of the enterobactin-deficient Escherichia coli strain SAB11 in

117 e cloned yiuABC operon restored growth of an enterobactin-deficient mutant Escherichia coli strain, 1

118 Escherichia coli 1017 (an enterobactin-deficient mutant) carrying this plasmid was

119 Thus, there is a functional link between enterobactin-dependent and catecholamine-dependent trans

120 These studies establish that bfeR encodes an enterobactin-dependent positive regulator of bfeA transc

121 MceIJ recognizes all glycosylated enterobactin derivatives formed by the MccE492 gene clus

122 a bfeA mutant demonstrated that induction by enterobactin did not require BfeA receptor-mediated upta

123 The monomeric component of enterobactin, dihydroxybenzoylserine (DHBS), and the S2

124 tolC mutants unable to synthesize enterobactin display no growth or morphological defects,

125 ed that the P43 mutant was unable to secrete enterobactin efficiently.

126 Addition of aferric, but not iron-saturated, enterobactin elicits a dose-dependent increase in secret

127 res perturb labile cellular iron pools, only enterobactin elicits interleukin-8 secretion, suggesting

128 nterica modify the tricatecholic siderophore enterobactin (Ent) by glucosylation of three aryl carbon

129 We recently reported that IroB is an enterobactin (Ent) C-glucosyltransferase, converting the

130 The siderophore enterobactin (Ent) is produced by enteric bacteria to me

131 The siderophore enterobactin (Ent) is produced by many species of enteri

132 We report that enterobactin (Ent), a catecholate siderophore expressed

133 Here we demonstrate that enterobactin (Ent), a catecholate siderophore released b

134 Siderophores, such as enterobactin (Ent), are small molecules that can be sele

135 sequesters the Escherichia coli siderophore enterobactin (Ent), preventing E. coli from acquiring ir

136 ive bacteria secrete siderophores, including enterobactin (Ent).

137 ilizes iron-scavenging siderophores, such as enterobactin (Ent).

138 e design and syntheses of monofunctionalized enterobactin (Ent, L- and D-isomers) scaffolds where one

139 The siderophore ferric enterobactin enters Escherichia coli through the outer m

140 ctin esterase BesA is less specific than the enterobactin esterase Fes; BesA can hydrolyze the trilac

141 cient strain of E. coli H1187 (fepA-) or the enterobactin esterase-deficient derivative of E. coli K-

142 h those of Fes, the previously characterized enterobactin esterase.

143 he notion that these proteins have a role in enterobactin excretion as well as synthesis.

144 Ferric enterobactin (FeEnt) acquisition is a highly efficient a

145 rt and used it to study the uptake of ferric enterobactin (FeEnt) by Escherichia coli FepA.

146 The ferric enterobactin (FeEnt) receptor CfrA is present in the maj

147 with 50- to 100-fold-lower affinity than Fe-enterobactin (FeEnt), despite an identical metal center,

148 We characterized the uptake of ferric enterobactin (FeEnt), the native Escherichia coli ferric

149 Binding of the ligand, ferric enterobactin (FeEnt), to the purified spin-labeled prote

150 chromatography assay the K(d) of the ferric enterobactin-FepB binding reaction was approximately 135

151 ome A, ferrioxamine B), catecholates (ferric enterobactin, ferric corynebactin) and eukaryotic bindin

152 y distinct siderophores including alcaligin, enterobactin, ferrichrome, and desferrioxamine B.

153 was found that fit was not able to transport enterobactin, ferrichrome, transferrin, and lactoferrin

154 ve putative transporters specific for BB and enterobactin (FeuA), 3,4-DHB and PB (FatB), PB (FpuA), s

155 barrier of Gram-negative pathogens utilizing enterobactin for iron acquisition.

156 nsporting the catecholate siderophore ferric enterobactin from the outer to the inner membrane in Gra

157 ate geometries using the gallic complexes of enterobactin: [Ga(III)(Ent)](3)(-) and [Ga(III)(H(3)Ent)

158 quely defined the binding pocket for gallium enterobactin (GaEnt).

159 kDa cytoplasmic membrane protein) within the enterobactin gene cluster were investigated by measuring

160 genetic organization of the Escherichia coli enterobactin gene cluster, in which the enterobactin bio

161 osin, pyoluteorin, mycosubtilin, nikkomycin, enterobactin, gramicidin, and several proteins from the

162 the siderophore enterochelin (also known as enterobactin) greatly enhanced detectable cross-linking

163 ere highly correlated with the results of an enterobactin growth promotion assay and a PCR analysis u

164 1DeltaiucA, with the relative activity being enterobactin > aerobactin > yersiniabactin > salmochelin

165 s) scaffolds where one catecholate moiety of enterobactin houses an alkene, aldehyde, or carboxylic a

166 inds the Fe(III) complex of the tetradentate enterobactin hydrolysis product bis(2,3-dihydroxybenzoyl

167 tions, and rationalize reports on the use of enterobactin hydrolysis products by C. jejuni, Vibrio ch

168 PDGC system transports both vibriobactin and enterobactin in Escherichia coli.

169 almochelin appears to be more important than enterobactin in the colonization of the spleen and liver

170 ys a role in the TonB2-mediated transport of enterobactin in this human pathogen.

171 of gut mucus showed that CAT4 hyperexcreted enterobactin in vivo, effectively rendering the catechol

172 he small molecule iron chelator siderophore, enterobactin, in response to intracellular iron depletio

173 protein recognition and binding of modified enterobactin increase the significance of understanding

174 ia coli strains expressing various levels of enterobactin induce an enterobactin-mediated proinflamma

175 R mutants was completely unresponsive to the enterobactin inducer.

176 In this study, enterobactin-inducible bfeA transcription was shown to b

177 Ferric enterobactin inhibited H8 binding to E. coli FepA (50% i

178 Ferric enterobactin is a catecholate siderophore that binds wit

179 Enterobactin is a secondary metabolite produced by Enter

180 The siderophore enterobactin is a triscatechol derivative of a cyclic tr

181 The Escherichia coli siderophore enterobactin is assembled from 2,3-dihydroxybenzoate (2,

182 ebA-F) resembling that for enterobactin, yet enterobactin is not produced.

183 This response to purified enterobactin is potentiated by recombinant siderocalin a

184 rate, showed higher affinity for both ferric enterobactin (K(d) = 30 nM) and ferric enantioenterobact

185 (III) complex of the hexadentate siderophore enterobactin (Kd approximately 0.4 +/- 0.1 microM), pref

186 ptides, resides 15 kb away from the putative enterobactin-like biosynthetic gene cluster (aebG).

187 sted that V. harveyi may produce amphiphilic enterobactin-like siderophores.

188 The proximity of this FACL gene to the enterobactin-like synthetase suggested that V. harveyi m

189 on, synthesis, recognition, and transport of enterobactin make it perhaps the best understood of the

190 Thus, aferric enterobactin may be a proinflammatory signal for respira

191 Enterobactin may be utilized for delivering molecular ca

192 he function of CfrA, which diminished ferric enterobactin-mediated growth promotion under iron-restri

193 n assays indicated that mutants defective in enterobactin-mediated iron acquisition were unable to co

194 ing various levels of enterobactin induce an enterobactin-mediated proinflammatory response.

195 C-terminal decapeptide and monoglycosylated enterobactin (MGE) requires cleavage of the alpha,beta b

196 synthesis of functionalized monoglucosylated enterobactin (MGE).

197 siderophore into mono-, di-, and triglucosyl enterobactins (MGE, DGE, and TGE, respectively).

198 bond-forming condensation (C) domain of the enterobactin NRPS EntF was excised from the multidomain

199 ane grow normally, whereas cells that import enterobactin only to the periplasm become morphologicall

200 t IroC is not required for utilization of Fe-enterobactin or Fe-salmochelin, as had been previously s

201 n genes required for the synthesis of either enterobactin or yersiniabactin were constructed, and the

202 iation with a bacterial siderophore, such as enterobactin, or a postulated mammalian siderophore.

203 etic and transport genes lie adjacent to the enterobactin outer membrane receptor, fepA, and the util

204 The biosynthesis and utilization of enterobactin permits many Gram-negative bacteria to thri

205 These studies demonstrate that the native enterobactin platform provides a means to effectively de

206 s of K. pneumoniae isolates were identified: enterobactin positive (Ent(+)) (81%), enterobactin and y

207 Oxidation of enterobactin produced a colored precipitate suggestive o

208 ypothesized that the catecholate siderophore enterobactin, produced by Enterobacteriaceae, serves as

209 mately 500-fold improvement in reconstituted enterobactin production activity.

210 ng and selected for their ability to support enterobactin production.

211 ferric complexes and the two forms of ferric enterobactin provided bond distances and disorder factor

212 The ferric enterobactin receptor CfrA not only is responsible for h

213 In this study, two candidate enterobactin receptor genes, irgA (VC0475) and vctA (VCA

214 pertussis was confirmed using wild-type and enterobactin receptor mutant strains in similar competit

215 The BfeA enterobactin receptor was found to not be involved direc

216 the recently characterized gonococcal ferric enterobactin receptor, exhibited extremely rapid phase v

217 tibodies raised against the Escherichia coli enterobactin receptor, FepA, recognized FetA in Western

218 s allowed the characterization of the ferric enterobactin receptor, previously named CfrA.

219 No growth recovery was observed for the enterobactin receptor-deficient strain of E. coli H1187

220 We now report that FetA functions as an enterobactin receptor.

221 morphological defects, and adding exogenous enterobactin recreates these aberrations, implicating th

222 ing one or more outer membrane receptors for enterobactin-related compounds, demonstrated that the P4

223 e catecholate siderophores bacillibactin and enterobactin requires the FeuABC importer and the YusV A

224 ynthesis of tryptophan, p-aminobenzoate, and enterobactin, respectively, and are expected to share a

225 ses BesA and Fes hydrolyze bacillibactin and enterobactin, respectively, as well as the corresponding

226 additional inability of CAT40 to synthesize enterobactin resulted in a 1000-fold better colonization

227 tin deficient), and hvKP1DeltaentBDeltairp2 (enterobactin, salmochelin, and yersiniabactin deficient)

228 rast to aerobactin, the inability to produce enterobactin, salmochelin, or yersiniabactin individuall

229 lin (Amx) are linked to a monofunctionalized enterobactin scaffold via a stable poly(ethylene glycol)

230 gnizes, transports, and utilizes derivatized enterobactin scaffolds.

231 t, is expressed inducibly at high levels and enterobactin-secreting respiratory flora is rare, sugges

232 t P43 is a critical component of the E. coli enterobactin secretion machinery and provides a rational

233 rized with the exception of the mechanism of enterobactin secretion to the extracellular environment.

234 in, enterobactin and the synthetic analogs d-enterobactin, SERGlyCAM and d-SERGlyCAM has determined t

235 Utilization of the enterobactin siderophore by the respiratory pathogens Bo

236 showing early induction of the alcaligin and enterobactin siderophore systems, and delayed induction

237 of the mutants revealed that the lack of the enterobactin siderophore was linked to a reduced CPS exp

238 cue facilitates E. coli biosynthesis of the enterobactin siderophore, allowing E. coli growth and bi

239 ture determination of a suite of eight amphi-enterobactin siderophores composed of the cyclic lactone

240 arrying site-directed mutations of genes for enterobactin synthesis (DeltaentA::Cm; strain CAT0), fer

241 Although the systems responsible for enterobactin synthesis and acquisition are well characte

242 SAB11 transductants in which growth but not enterobactin synthesis was restored on media containing

243 ceptor for heme, an entB mutant defective in enterobactin synthesis, and a shuA entB double mutant ea

244 oli enzymes necessary for the final stage of enterobactin synthesis, are released by osmotic shock.

245 the chromosomal region of genes involved in enterobactin synthesis, shows strong homology to the 12-

246 including iron transport, transferases, and enterobactin synthesis.

247 Here we report the first reconstitution of enterobactin synthetase activity from pure protein compo

248 we have used the two-module Escherichia coli enterobactin synthetase as a model system.

249 ) di-domain fragment of the Escherichia coli enterobactin synthetase EntF NRPS subunit.

250 analysis of EntE, one of six enzymes in the enterobactin synthetase gene cluster.

251 M sample of a 37 kDa fragment of the E. coli enterobactin synthetase module EntF, for which high-reso

252 receptor), hlyA (alpha-hemolysin), or entF (enterobactin synthetase subunit).

253 eliminating the requirement for EntD in the enterobactin synthetase.

254 ogous to EntE and EntB from Escherichia coli enterobactin synthetase; VibE activates DHB as the acyl

255 The enterobactin system for iron transport in Escherichia co

256 In this study, the contribution of the enterobactin system to the fitness of B. pertussis was c

257 ine scaffold via glycine spacers, whereas in enterobactin the iron-binding moieties are directly atta

258 hat CueO protects E. coli cells by oxidizing enterobactin, the catechol iron siderophore of E. coli,

259 n by sequestering ferric iron complexes with enterobactin, the conserved E. coli siderophore.

260 dramatically, during the transport of ferric enterobactin, the natural ligand of FepA.

261 lded state, the isolated domain binds ferric enterobactin, the siderophore ligand of FepA, with an af

262 owed that the ahpC mutant secreted much less enterobactin, the siderophore that chelates and transpor

263 Enterobactin, the tris-(N-(2,3-dihydroxybenzoyl)serine)

264 derestimated the affinity of FepA for ferric enterobactin: the interaction between the protein and th

265 duction of the Escherichia coli NRPS product enterobactin to map the surface of the aryl carrier prot

266 Siderocalin has been shown to deliver enterobactin to other mammalian cell types, exogenously

267 cells, and siderocalin increases delivery of enterobactin to the intracellular compartment.

268 protein complex that attaches C-glycosylated enterobactins to the C-terminal serine residue of both a

269 or complete inhibition of the rate of ferric enterobactin transport across the OM.

270 results demonstrate that the E. coli ferric enterobactin transport machinery identifies and delivers

271 f FepA are superficially dispensable: ferric enterobactin transport occurred without them, at levels

272 Enterobactin transport requires TonB and is independent

273 E. coli K-12 derivatives defective in ferric enterobactin transport reveal that the enhanced antibact

274 In the same assay, the E. coli enterobactin transport system, FepBDGC, allowed the util

275 scherichia coli is a component of the ferric enterobactin transport system.

276 Strikingly, heme acquisition, ferric-enterobactin transport, and pyoverdine biosynthesis gene

277 erved in the distribution of porin A, ferric enterobactin transport, and strain genotypes among vacci

278 riplasmic binding protein VctP did not alter enterobactin transport, but eliminated growth stimulatio

279 hance the overall affinity or rate of ferric enterobactin transport, supporting the conclusion that t

280 is strain requires the outer membrane ferric enterobactin transporter FepA.

281 the extent to which the Gram-negative ferric enterobactin uptake and processing machinery recognizes,

282 ol siderophores, including bacillibactin and enterobactin, use 2,3-DHBA as a biosynthetic subunit.

283 growth of single crystals containing ferric enterobactin using racemic crystallization, a method tha

284 Ferric enterobactin utilization by Bordetella bronchiseptica an

285 source of iron, demonstrating that effective enterobactin utilization is bfeR dependent.

286 Omega insertion within fetB abolished ferric enterobactin utilization without causing a loss of ferri

287 of these genes did not significantly impair enterobactin utilization, but a strain defective in both

288 ut can also use the catechol xenosiderophore enterobactin via the BfeA outer membrane receptor.

289 d together with 2,3-dihydroxybenzoic acid or enterobactin was able to induce a Phi(cueO-lacZ) operon

290 K-12 JW0576 (fes-), or when the D-isomer of enterobactin was employed.

291 IroN mediates utilization of the siderophore enterobactin was obtained, thereby establishing IroN as

292 bility of the irgA vctA double mutant to use enterobactin was restored.

293 ynthesize but cannot secrete the siderophore enterobactin, which collects in the periplasm.

294 n low iron conditions accumulate periplasmic enterobactin, which impairs bacterial morphology, possib

295 ation of IroE suggest that it hydrolyzes apo enterobactins while they are being exported.

296 All the transport proteins bound ferric enterobactin with high affinity (Kd </= 100 nM) and tran

297 FA1090 FetA specifically bound 59Fe-enterobactin, with a Kd of approximately 5 microM.

298 fied FepB also adsorbed the apo-siderophore, enterobactin, with comparable affinity (K(d) = 60 nM) bu

299 erophore NRPSs that synthesize vibriobactin, enterobactin, yersiniabactin, pyochelin, and anguibactin

300 S) gene cluster (aebA-F) resembling that for enterobactin, yet enterobactin is not produced.