ライフサイエンスコーパス: enzymatic assay

1 cis-aconitate decarboxylating activity in an enzymatic assay.

2 tion in saliva was performed using an immune enzymatic assay.

3 ridine (rac)-5 was discovered by HTS using a enzymatic assay.

4 ein-1 levels were determined using an immuno-enzymatic assay.

5 le BioC preparations were too low for direct enzymatic assay.

6 omponents typically included in the standard enzymatic assay.

7 rum fibrinogen level was also measured using enzymatic assay.

8 sequence analysis and functionally active by enzymatic assay.

9 hate-stimulated protein kinase A by means of enzymatic assay.

10 ase by more than 4-fold over basal levels by enzymatic assay.

11 tease activity was measured by a fluorescent enzymatic assay.

12 the official method of analysis is based on enzymatic assay.

13 and evaluation of the library in a BoNT/A LC enzymatic assay.

14 then submitted directly to an in vitro FIXa enzymatic assay.

15 ith nonadsorbing interfaces in droplet-based enzymatic assays.

16 idylcholine as the sole carbon source and in enzymatic assays.

17 creening for this condition with blood-based enzymatic assays.

18 autoantibodies, lymphocyte stimulation, and enzymatic assays.

19 ough molecular dynamics (MD) simulations and enzymatic assays.

20 phy-mass spectrometry), NMR spectroscopy and enzymatic assays.

21 rifugation and fractions analyzed by BNGE or enzymatic assays.

22 these inhibitors was confirmed with in vitro enzymatic assays.

23 ith oxidative stress, a finding supported by enzymatic assays.

24 ion exchange liquid chromatography assays or enzymatic assays.

25 ated using Western blot analysis, ELISA, and enzymatic assays.

26 oss of RPE65-dependent isomerase activity by enzymatic assays.

27 d ThnT, respectively, in a series of coupled enzymatic assays.

28 ruvate, and lactate) using microfluorometric enzymatic assays.

29 ed well with the IC(50) values obtained from enzymatic assays.

30 tested these molecules in tissue culture and enzymatic assays.

31 itable conditions had to be selected for the enzymatic assays.

32 ange of Ki previously obtained from in vitro enzymatic assays.

33 s further confirmed by mass spectrometry and enzymatic assays.

34 calorimetry and with the IC(50) values from enzymatic assays.

35 and fully restored tissue factor function in enzymatic assays.

36 ll types, as measured by flow cytometric and enzymatic assays.

37 urokinase inhibitors in both human and mouse enzymatic assays.

38 romatography, mass spectrometry, and earlier enzymatic assays.

39 fluorescent tags and with radioactive-based enzymatic assays.

40 experiments using liquid chromatography and enzymatic assays.

41 ling (SDSL) coupled to EPR spectroscopy, and enzymatic assays.

42 y eight acyl-PTMs in vivo and after in vitro enzymatic assays.

43 sign of AZ1 analogs with enhanced potency in enzymatic assays.

44 e the development of more versatile in vitro enzymatic assays.

45 expected functionality in both chemical and enzymatic assays.

46 y, bacterial profiling, and immunometric and enzymatic assays.

47 moderately decreased sensitivity to LAMTP in enzymatic assays (3.6-fold decreased sensitivity).

48 C1 and HDAC8 was then determined by in vitro enzymatic assays, after which the cytotoxicity was evalu

49 th an IC50 value of 0.02 muM in a PDGF-Rbeta enzymatic assay also showing activity against PDGF-R dep

50 In vitro enzymatic assays also revealed that the ManS enzyme from

51 1a, which had an IC(50) of 1.2 nM in the Src enzymatic assay, an IC(50) of 100 nM for the inhibition

52 These groups include: enzymatic assays; analysis of respiration of mammalian a

53 unds was achieved using a fluorescence-based enzymatic assay and a substrate phosphorylation assay in

54 xy group, had an IC(50) of 3.8 nM in the Src enzymatic assay and an IC(50) of 940 nM for the inhibiti

55 rapid activation in a carboxylesterase (CPY) enzymatic assay and favorable ADME properties, while als

56 uman MetAP1 (HsMetAP1), as confirmed by both enzymatic assay and high-resolution x-ray crystallograph

57 by using commercially available fluorometric-enzymatic assay and liquid chromatography/mass spectrome

58 ingly, caspase-3 activation as determined by enzymatic assay and mitochondrial cytochrome c release d

59 Enzymatic assay and nuclear magnetic resonance (NMR) ana

60 omposition (GAD(65) and GAD(67)) measured by enzymatic assay and quantitative immunoblotting.

61 gated in these disparate conditions by using enzymatic assay and reverse transcription-PCR.

62 To date, an enzymatic assay and two chemical assays have been develo

63 hibits SHP2 with an IC(50) = 0.031 muM in an enzymatic assay and with an IC(50) = 2.6 muM in human pa

64 pathology typical of steatosis, assessed by enzymatic assays and analysis of liver sections.

65 d proteins remain bioactive, as evidenced by enzymatic assays and antigen-antibody binding experiment

66 als of growth, were analyzed by chemical and enzymatic assays and Fourier transform infrared spectros

67 Enzymatic assays and immunoblotting confirm that pristim

68 n of oncogenic phenotypes as demonstrated in enzymatic assays and in a panel of cancer cell lines, in

69 ectivity for Aurora A over Aurora B, both in enzymatic assays and in cellular phenotypic assays.

70 ctivity in vitro and in vivo, as revealed by enzymatic assays and increased sensitivity to the Acc1-s

71 l-[U-(13)C, (15)N]serine as well as in vitro enzymatic assays and liquid chromatography-tandem mass s

72 Pathogenicity was further supported by enzymatic assays and mass spectroscopy on recombinant pr

73 ght scattering analysis, circular dichroism, enzymatic assays and molecular modeling approaches have

74 he results highlighted the inadequacy of the enzymatic assays and of the HPLC-RI method to analyse la

75 The enzymatic assays and panel of glycosylation mutants used

76 A-3 were validated in vitro using individual enzymatic assays and reconstitution of the three-step pa

77 The metabolite profiling was confirmed by enzymatic assays and replicated in a cohort of 11 GCK-MO

78 Using enzymatic assays and steady-state fluorescence emission,

79 nds display enhanced potency against LpxC in enzymatic assays and superior antibiotic activity agains

80 served for inhibition of both purified RT in enzymatic assays and viral replication in cell-based ass

81 Results from both enzymatic assays and Western blots of cell extracts from

82 earts by real-time RT-PCR, Western blotting, enzymatic assay, and histological assays.

83 lls as a vascular model system, for in vitro enzymatic assay, and in mass spectrometry analysis.

84 h normal and reverse-phase, UV spectroscopy, enzymatic assays, and acid hydrolysis.

85 m-made high-throughput phenotyping platform, enzymatic assays, and gas chromatography-mass spectromet

86 e injected animals by Western blot analysis, enzymatic assays, and immunofluorescence measurements.

87 the nano- to femtomole scale, in alternative enzymatic assays, and in kinetic isotope effect studies

88 ty was confirmed through whole-cell and pure enzymatic assays, and it is specific for cortisol.

89 ion of the respective peptides and proteins, enzymatic assays, and LC-MS analyses, we determined that

90 lyses, along with site-directed mutagenesis, enzymatic assays, and molecular dynamics simulations, we

91 Using protein modeling, enzymatic assays, and mutant embryos, we determined that

92 Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose t

93 Site-directed mutagenesis experiments, enzymatic assays, and x-ray crystallographic analyses su

94 rom examining phenotypic variation, in vitro enzymatic assays, and yeast genetic experiments) to expl

95 26S proteasome by these derivatives using an enzymatic assay are also reported.

96 All three enzymatic assays are based on the same observed phenomen

97 Enzymatic assays are widely employed to characterize imp

98 antitative RT-PCR (qRT-PCR) mRNA expression, enzymatic assay aromatase activity, scratch assay cell m

99 eptide ligand complex has been developed for enzymatic assays as an alternative to immuno-based detec

100 A new enzymatic assay, based on photometric detection of ammon

101 e down and increase the throughput of glycoT enzymatic assays because traditional methods require lar

102 slightly less active than 1a in the p38alpha enzymatic assay but displays a superior pharmacokinetic

103 the HRP:sol-gel material was demonstrated by enzymatic assays by using I(-), guaiacol, and ABTS as su

104 Each droplet represented an individual enzymatic assay carried out in a sub-nanoliter (0.8 nL)

105 Enzymatic assays, chemical proteomic profiling, kinome-w

106 he cage and Trp-containing hexapeptide under enzymatic assay conditions.

107 actions, spectroelectrochemical studies, and enzymatic assays conducted in the presence of DT-diaphor

108 Mutational and enzymatic assays confirm the importance of this lysine i

109 Enzymatic assays confirmed a difference in urease expres

110 Enzymatic assays confirmed its activity of beta-galactos

111 Furthermore, enzymatic assays confirmed that AK transforms galaxolide

112 Immunoblot and enzymatic assays confirmed that the increases in AKR1C m

113 In vitro enzymatic assays confirmed that the JmjC domain of Rph1

114 Enzymatic assays confirmed that the predicted membrane t

115 Significantly, enzymatic assays confirmed that this protein is necessar

116 A novel enzymatic assay coupled to formation of fluorescent chro

117 roach was taken featuring organic synthesis, enzymatic assaying, crystallography, and modeling includ

118 Enzymatic assays demonstrate that LpxJ and homologues in

119 Enzymatic assays demonstrate that mutants harboring the

120 Mass spectrometry-based lipidomics and enzymatic assays demonstrate the targeting mechanisms re

121 Direct enzymatic assays demonstrated a comparable reduction in

122 Indeed, enzymatic assays demonstrated that AtGA2ox7 and AtGA2ox8

123 In vitro enzymatic assays demonstrated that recombinant HasB2 is

124 Chemical and enzymatic assays demonstrated that the bacterium release

125 lly, a combination of in silico and in vitro enzymatic assays demonstrated the metabolism of these ph

126 An enzymatic assay determined the impact of BAC over-expres

127 script and metabolite analyses together with enzymatic assays determined that the biosynthesis of ant

128 assay could also be used to complement a PTx-enzymatic assay, developed recently, and together they m

129 f inhibition of the HDAC activity in an HDAC enzymatic assay differed strikingly on their effects on

130 effect-directed assays, like bioassays or an enzymatic assay, directly linked with chromatography and

131 Moreover, unlike enzymatic assays (e.g., elastase), there is no confusion

132 onventional methods, including fluorescence, enzymatic assays (ELISA), and quantitative real-time PCR

133 An enzymatic assay established that 2a was an ATP-competiti

134 Finally, enzymatic assays established that both CmPP36 and Delta

135 sensitivity and 88% specificity, whereas the enzymatic assay exhibited 83% sensitivity and 80% specif

136 Imaging results were corroborated by enzymatic assays, flow cytometry, and histopathologic an

137 uble nuclear fractions from purified nuclei, enzymatic assays, fluorescence microscopy, affinity chro

138 th 3:3 stoichiometry, which was confirmed by enzymatic assays, fluorescent labeling, and small-angle

139 We have developed a continuous enzymatic assay for adenosine that facilitates high-thro

140 agents, making it suitable as a quantitative enzymatic assay for the activity of individual chitin sy

141 With the aim of developing a specific enzymatic assay for the detection and quantification of

142 sing 1D (31)P-NMR spectroscopy and a coupled enzymatic assay for the determination of inorganic phosp

143 In this study, an enzymatic assay for the determination of trypsin inhibit

144 Enzymatic assays for ATP and lactate production confirme

145 Enzymatic assays for catalase and dihydrolipoamide dehyd

146 med using immunoflourescence, AlphaLISA, and enzymatic assays for cathepsin B inhibition.

147 tions, two new simple and robust fluorogenic enzymatic assays for MGL and AHCY were developed and app

148 isolates from infected mice were screened by enzymatic assays for SpeB and the platelet-activating fa

149 nd stavudine (d4T) were determined, using an enzymatic assay, for 5 HTLV-1 isolates and for reference

150 Our enzymatic assays further reveal a robust regulatory mech

151 The PLP enzymatic assay has a lower limit of detection of 14.8 n

152 The enzymatic assay has also indicated the existence of an a

153 as 11-cis-retinol dehydrogenase (cRDH), and enzymatic assays have confirmed the isolation of an acti

154 Finally, enzymatic assays identified ceramide synthase as the mec

155 We developed a robust enzymatic assay in a 1536-well plate format.

156 distinguished by a simple, highly sensitive enzymatic assay in cell lysates.

157 , while catalytic activity was determined by enzymatic assay in the presence (total activity) and the

158 henazine reduction in Pseudomonas aeruginosa Enzymatic assays in cell-free lysate, together with crud

159 Enzymatic assays in human cells revealed that STEAP1 pro

160 In vitro enzymatic assays in patient fibroblast cells showed defi

161 ophotometric experiments in combination with enzymatic assays in solution showed that TsdB acts as an

162 ratio) bilayers using (2)H-NMR and specific enzymatic assays in the absence or presence of 7.5 mol %

163 e as physiologically-relevant substrates for enzymatic assays in vitro.

164 Gene expression and enzymatic assays indicate that erythroid 5-aminolevulini

165 Site-directed mutagenesis and enzymatic assays indicate that the interaction is fundam

166 ings, our biophysical experiments and kinase enzymatic assays indicate that the L834R mutation causes

167 m1 sequence was homologous to chitinase, and enzymatic assays indicated a 3-5-fold increase in chitin

168 Enzymatic assays indicated that the (S)- and (R)-isomers

169 ompounds have been reported to inhibit IN in enzymatic assays, inhibition is not always correlated wi

170 An enzymatic assay, involving collection of a phenolic subs

171 planar chromatogram with in situ biological/enzymatic assays is a powerful bioanalytical screening t

172 red with the measurements using a commercial enzymatic assay kit.

173 hose recorded using a commercially available enzymatic assay kit.

174 nce a large number of commercially available enzymatic assay kits utilize NADH in their detection, th

175 n due to pre-absorption by the product of an enzymatic assay leads to quenching of the PL of quantum

176 Enzymatic assays led to the discovery that PA4872 decarb

177 Enzymatic assays measured catalytic activity of cathepsi

178 of this putative iron-entry site by means of enzymatic assays, MS, and X-ray crystallography.

179 the use of an integrative strategy including enzymatic assays, mutant analysis, metabolic engineering

180 Immunoblotting, quantitative RT-PCR, enzymatic assays, nucleotide sugar, and sugar phosphate

181 croscopy and gel electrophoresis, as well as enzymatic assay of B(12)-dependent diol dehydratase.

182 on using in vivo bioluminescence imaging and enzymatic assay of lung homogenates.

183 Apoptosis was assessed by enzymatic assay of myocardial caspase 3 and TUNEL staini

184 This was confirmed by enzymatic assay of the partially purified enzyme with un

185 Enzymatic assay of these mutant alleles demonstrate that

186 Its attributes are ideal for enzymatic assays of alkaline phosphatase for both basic

187 Enzymatic assays of both recombinant proteins showed try

188 t nervous system by using Northern blots and enzymatic assays of brain and subfractionated primary ne

189 In addition, enzymatic assays of choline kinase activity in cells wer

190 Previous enzymatic assays of crude cell extracts indicated that a

191 gene expression at mRNA and protein levels, enzymatic assays of gene function, clonal analysis of PC

192 uenzae YbaK using site-directed mutagenesis, enzymatic assays of isosteric substrates and functional

193 1 encodes a pectin methylesterase (PME), and enzymatic assays of QRT1 expressed in Escherichia coli i

194 In addition, enzymatic assays of the wild-type proteins as well as se

195 Phenotypic and enzymatic assays on grafted plants indicated that mRNAs

196 esults from HPLC and MS analysis of in vitro enzymatic assays on stereochemically defined SP substrat

197 Enzymatic assays on TA0175 revealed that this enzyme cat

198 Enzymatic assays on the insect-cell-expressed human GALN

199 Current standard methods, such as enzymatic assays or high-performance liquid chromatograp

200 We used a combination of enzymatic assays, PA-binding assays, and circular dichro

201 us tissues for metabolism studies, including enzymatic assays, paper chromatography, and high-perform

202 In the present study, direct enzymatic assays performed with alternative substrates a

203 Enzymatic assays performed with APC purified from strain

204 l toolbox that includes lipid substrates for enzymatic assays, potent inhibitors, and chemical probes

205 have automated bacterial transformation and enzymatic assay protocols, showing that such a system en

206 he standard technique, namely, a homogeneous enzymatic assay (R(2) = 0.97).

207 Enzymatic assays, reductive titrations, and circular dic

208 urrently available radioreceptor binding and enzymatic assays require extensive sample preparation an

209 LIS assay was completely consistent with the enzymatic assay results.

210 A mass spectrometry based enzymatic assay revealed that two compounds, N(6)-benzyl

211 threonine are substrates for T. maritima TA, enzymatic assays revealed a strong preference for L-allo

212 nalysis of MJ0936 against a panel of general enzymatic assays revealed catalytic activity toward bis-

213 Western blots and arginase enzymatic assays revealed high-level expression of cytos

214 In vitro enzymatic assays revealed that ADP-ribose and NADH are p

215 In vitro enzymatic assays revealed that fsh poorly converts subst

216 Complex I enzymatic assays revealed that the 3460A mutation result

217 Further in vitro enzymatic assays revealed that the 9-modified neuraminic

218 Enzymatic assays revealed that the D6 D386N mutation aff

219 Enzymatic assays revealed that the distribution patterns

220 In vitro enzymatic assays revealed that the mutant enzymes had in

221 are expressed in BoHV-4 infected cells, but enzymatic assays revealed that the spliced form is not a

222 LDH/PK and colorimetric enzymatic assays revealed two promising sChoK inhibitor

223 Enzymatic assays revealed vast differences in PTP cataly

224 h a combination of yeast complementation and enzymatic assays, show impaired enzymatic activity and s

225 Enzymatic assays showed a basal level of phosphatase act

226 nding assays with active site inhibitors and enzymatic assays showed that Ab58 is a competitive inhib

227 Enzymatic assays showed that glutamine, a signal of nitr

228 Enzymatic assays showed that RH1 was an in vitro substra

229 Enzymatic assays showed that T-705 and T-1105 are poor s

230 In addition, the results of enzymatic assays showed that the level of GDH during ano

231 In vitro enzymatic assays showed that the purified S55F mutant en

232 Furthermore, in vitro enzymatic assays showed that the three AMPs are transfer

233 in vitro enzymatic assays showed that this substitution reduces t

234 in expressed in Escherichia coli followed by enzymatic assay, showed that this enzyme is active on C8

235 ing data from Western and Northern blots and enzymatic assays showing that both proteins are widely e

236 glucopeptides were synthesized and tested in enzymatic assays, showing a common minimal epitope.

237 Enzymatic assay shows the two fusions to have similar re

238 Enzymatic assays, sucrose density gradient centrifugatio

239 ard the presence of inactive mutants than in enzymatic assays, suggesting a weak coupling of ATPase a

240 established and validated a straightforward enzymatic assay suitable for high-throughput screening a

241 We established a cell-based LIPG enzymatic assay system to measure the inhibitory effect

242 The enzymatic assay targets alanine and employs alanine tran

243 Here we describe an enzymatic assay that allowed us to monitor Pmt4 activity

244 Also described is an array-based enzymatic assay that can be used to sequence portions of

245 The use of a drug-inducible enzymatic assay that depletes the Golgi-associated pool

246 Described in this study is an enzymatic assay that determines levels of homocysteine i

247 ions of that study were based on an indirect enzymatic assay that was not specific for (6-4) photopro

248 Here, we optimized two colorimetric-based enzymatic assays that detect the ureido moiety of the DH

249 ising molecular reagents for high-throughput enzymatic assays that successfully bridge the gap betwee

250 Using an in vitro enzymatic assay, the recombinant TPPE was able to proces

251 In in vitro enzymatic assays, the V555I mutation displayed the most

252 We have adapted a continuous enzymatic assay to a medium-throughput format and carrie

253 sed in Cos-7 cells were used to establish an enzymatic assay to detect and quantify the expression of

254 ic airway inflammation in asthma, we used an enzymatic assay to determine thrombin activity in bronch

255 luorescence staining, immunoblotting, and an enzymatic assay to evaluate the consequences of potentia

256 zyme and show the need for an ultrasensitive enzymatic assay to evaluate the potency of tight binding

257 attering (SAXS), chemical cross-linking, and enzymatic assays to enlarge our understanding of the int

258 cular dynamics simulations, mutagenesis, and enzymatic assays to explore the molecular basis of this

259 In this study, we use quantitative enzymatic assays to fully characterize C9Holo and a leuc

260 ion, fluorescence polarization and NMR-based enzymatic assays to investigate the impact of phosphomim

261 ship), medicinal chemistry, and experimental enzymatic assays to optimize a previously identified sma

262 those results with immunohistochemistry and enzymatic assays to unveil the relationship between some

263 By use of a reliable enzymatic assay together with X-ray crystallography as g

264 nts, metabolite quantification, and in vitro enzymatic assays under both saturating and non-saturatin

265 leukemia (RBL-2H3) mast cells using specific enzymatic assays, urea-polyacrylamide gel electrophoresi

266 tem to determine glucose was evaluated by an enzymatic assay using glucose oxidase, horseradish perox

267 the presence of biopolymers was assessed by enzymatic assay using natural and synthetic substrates.

268 Enzymatic assays using purified protein showed that unli

269 Enzymatic assays using recombinant proteins and D-cyclos

270 terase activity, as is evident from in vitro enzymatic assays using the purified protein.

271 me of the reagents, the dynamic range of the enzymatic assay was adjusted to 0-12 mM glucose and had

272 An enzymatic assay was developed to measure tetrahydrometha

273 An enzymatic assay was used to detect the activity distribu

274 one- and two-electron reduction in isolated enzymatic assays was studied, and their potential to gen

275 tion, ESI-MS/MS-based peptide sequencing and enzymatic assay we further demonstrate that human RBCs c

276 Using affinity pull-down and in vitro enzymatic assays, we confirmed the potent antibacterial

277 Using surface plasmon resonance and enzymatic assays, we found that albicin binds and stabil

278 Based on a phylogenetic analysis and enzymatic assays, we propose that the CaM/Ca(2+)-depende

279 Using in silico design followed by enzymatic assays, we show that Abz and EDDnp significant

280 ing, quantitative RT-PCR, FACS analysis, and enzymatic assays, we show that miR221 is translocated vi

281 qRT-PCR, Western blotting, and enzymatic assays were performed to confirm the regulatio

282 Degenerate PCR and enzymatic assays were used to assess the presence of but

283 accumulation of peroxides in HU solutions by enzymatic assays, which explains the toxicity, as both N

284 e a significant level of metal activation in enzymatic assays, which is inconsistent with the behavio

285 High-throughput (HT) enzymatic assays, which typically rely on labeled compou

286 are highly potent inhibitors of DHODH in an enzymatic assay, while also inhibiting cancer cell growt

287 s with a reporter) or throughput inherent in enzymatic assays widely used for HTS.

288 pionamide (10a) inhibits PDHK in the primary enzymatic assay with an IC(50) of 13 +/- 1.5 nM, enhance

289 opanamide (14e) inhibits PDHK in the primary enzymatic assay with an IC(50) of 16 +/- 2 nM, enhances

290 nd to inhibit gamma-secretase in a cell-free enzymatic assay with an IC(50) of 2.1 microM, a value si

291 tivity of a series of N-acylhydrazones in an enzymatic assay with PA-Nter endonuclease, as well as in

292 pases 3, 6, 8, and 9, as measured both by an enzymatic assay with synthetic substrates and by detecti

293 Enzymatic assays with a recombinant soluble form of heps

294 Such coupling of enzymatic assays with electrophoretic separations on a m

295 Homology searches, as well as direct enzymatic assays with M. jannaschii, failed to reveal th

296 Subsequent enzymatic assays with the nonribosomal peptide synthetas

297 alone and their activities were validated by enzymatic assays with the recombinant proteins or by the

298 results from phylogenetic analysis, in vitro enzymatic assays, X-ray crystallography, and in vivo Mtb

299 Here, we employ enzymatic assays, X-ray crystallography, and molecular s

300 e been investigated using organic synthesis, enzymatic assays, X-ray crystallography, and thermodynam