ライフサイエンスコーパス: enzymatic assay

1 le BioC preparations were too low for direct enzymatic assay.

2 omponents typically included in the standard enzymatic assay.

3 and evaluation of the library in a BoNT/A LC enzymatic assay.

4 rum fibrinogen level was also measured using enzymatic assay.

5 sequence analysis and functionally active by enzymatic assay.

6 hate-stimulated protein kinase A by means of enzymatic assay.

7 then submitted directly to an in vitro FIXa enzymatic assay.

8 ase by more than 4-fold over basal levels by enzymatic assay.

9 tease activity was measured by a fluorescent enzymatic assay.

10 the amount of beta-galactosidase by standard enzymatic assay.

11 on in the liver determined by a quantitative enzymatic assay.

12 nodetection of A and B antigens and in vitro enzymatic assay.

13 cis-aconitate decarboxylating activity in an enzymatic assay.

14 tion in saliva was performed using an immune enzymatic assay.

15 the official method of analysis is based on enzymatic assay.

16 ridine (rac)-5 was discovered by HTS using a enzymatic assay.

17 ein-1 levels were determined using an immuno-enzymatic assay.

18 autoantibodies, lymphocyte stimulation, and enzymatic assays.

19 ough molecular dynamics (MD) simulations and enzymatic assays.

20 phy-mass spectrometry), NMR spectroscopy and enzymatic assays.

21 rifugation and fractions analyzed by BNGE or enzymatic assays.

22 these inhibitors was confirmed with in vitro enzymatic assays.

23 ith oxidative stress, a finding supported by enzymatic assays.

24 ion exchange liquid chromatography assays or enzymatic assays.

25 ated using Western blot analysis, ELISA, and enzymatic assays.

26 ling (SDSL) coupled to EPR spectroscopy, and enzymatic assays.

27 oss of RPE65-dependent isomerase activity by enzymatic assays.

28 d ThnT, respectively, in a series of coupled enzymatic assays.

29 ruvate, and lactate) using microfluorometric enzymatic assays.

30 ed well with the IC(50) values obtained from enzymatic assays.

31 tested these molecules in tissue culture and enzymatic assays.

32 itable conditions had to be selected for the enzymatic assays.

33 ange of Ki previously obtained from in vitro enzymatic assays.

34 s further confirmed by mass spectrometry and enzymatic assays.

35 y eight acyl-PTMs in vivo and after in vitro enzymatic assays.

36 calorimetry and with the IC(50) values from enzymatic assays.

37 and fully restored tissue factor function in enzymatic assays.

38 ll types, as measured by flow cytometric and enzymatic assays.

39 urokinase inhibitors in both human and mouse enzymatic assays.

40 romatography, mass spectrometry, and earlier enzymatic assays.

41 fluorescent tags and with radioactive-based enzymatic assays.

42 were evaluated by western blot analysis and enzymatic assays.

43 otein kinase (MAPK) was assessed by specific enzymatic assays.

44 y SDS-PAGE zymography, Western blotting, and enzymatic assays.

45 e the development of more versatile in vitro enzymatic assays.

46 y, bacterial profiling, and immunometric and enzymatic assays.

47 ith nonadsorbing interfaces in droplet-based enzymatic assays.

48 idylcholine as the sole carbon source and in enzymatic assays.

49 creening for this condition with blood-based enzymatic assays.

50 moderately decreased sensitivity to LAMTP in enzymatic assays (3.6-fold decreased sensitivity).

51 C1 and HDAC8 was then determined by in vitro enzymatic assays, after which the cytotoxicity was evalu

52 th an IC50 value of 0.02 muM in a PDGF-Rbeta enzymatic assay also showing activity against PDGF-R dep

53 In vitro enzymatic assays also revealed that the ManS enzyme from

54 Northern blot analysis and enzymatic assays also were carried out on various murine

55 1a, which had an IC(50) of 1.2 nM in the Src enzymatic assay, an IC(50) of 100 nM for the inhibition

56 These groups include: enzymatic assays; analysis of respiration of mammalian a

57 unds was achieved using a fluorescence-based enzymatic assay and a substrate phosphorylation assay in

58 xy group, had an IC(50) of 3.8 nM in the Src enzymatic assay and an IC(50) of 940 nM for the inhibiti

59 in intact parasites were evaluated by direct enzymatic assay and by immunoblotting.

60 (CAT) gene, whose expression was measured by enzymatic assay and by Northern blotting; a truncated ne

61 uman MetAP1 (HsMetAP1), as confirmed by both enzymatic assay and high-resolution x-ray crystallograph

62 by using commercially available fluorometric-enzymatic assay and liquid chromatography/mass spectrome

63 ingly, caspase-3 activation as determined by enzymatic assay and mitochondrial cytochrome c release d

64 Enzymatic assay and nuclear magnetic resonance (NMR) ana

65 omposition (GAD(65) and GAD(67)) measured by enzymatic assay and quantitative immunoblotting.

66 gated in these disparate conditions by using enzymatic assay and reverse transcription-PCR.

67 To date, an enzymatic assay and two chemical assays have been develo

68 pid mixtures using a combination of specific enzymatic assays and 31P and 2H NMR.

69 pathology typical of steatosis, assessed by enzymatic assays and analysis of liver sections.

70 d proteins remain bioactive, as evidenced by enzymatic assays and antigen-antibody binding experiment

71 als of growth, were analyzed by chemical and enzymatic assays and Fourier transform infrared spectros

72 Enzymatic assays and immunoblotting confirm that pristim

73 ectivity for Aurora A over Aurora B, both in enzymatic assays and in cellular phenotypic assays.

74 ctivity in vitro and in vivo, as revealed by enzymatic assays and increased sensitivity to the Acc1-s

75 Enzymatic assays and lipid supplementation studies showe

76 l-[U-(13)C, (15)N]serine as well as in vitro enzymatic assays and liquid chromatography-tandem mass s

77 ght scattering analysis, circular dichroism, enzymatic assays and molecular modeling approaches have

78 he results highlighted the inadequacy of the enzymatic assays and of the HPLC-RI method to analyse la

79 The enzymatic assays and panel of glycosylation mutants used

80 Enzymatic assays and polymerase chain reaction analysis

81 A-3 were validated in vitro using individual enzymatic assays and reconstitution of the three-step pa

82 The metabolite profiling was confirmed by enzymatic assays and replicated in a cohort of 11 GCK-MO

83 Using enzymatic assays and steady-state fluorescence emission,

84 nds display enhanced potency against LpxC in enzymatic assays and superior antibiotic activity agains

85 served for inhibition of both purified RT in enzymatic assays and viral replication in cell-based ass

86 Enzymatic assays and western blot analysis were carried

87 Results from both enzymatic assays and Western blots of cell extracts from

88 Enzymatic assays and western blotting confirmed that the

89 earts by real-time RT-PCR, Western blotting, enzymatic assay, and histological assays.

90 lls as a vascular model system, for in vitro enzymatic assay, and in mass spectrometry analysis.

91 PI-PLC activity was determined by enzymatic assay, and protein expression of the PLC isozy

92 h normal and reverse-phase, UV spectroscopy, enzymatic assays, and acid hydrolysis.

93 e injected animals by Western blot analysis, enzymatic assays, and immunofluorescence measurements.

94 the nano- to femtomole scale, in alternative enzymatic assays, and in kinetic isotope effect studies

95 Using protein modeling, enzymatic assays, and mutant embryos, we determined that

96 Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose t

97 Site-directed mutagenesis experiments, enzymatic assays, and x-ray crystallographic analyses su

98 rom examining phenotypic variation, in vitro enzymatic assays, and yeast genetic experiments) to expl

99 26S proteasome by these derivatives using an enzymatic assay are also reported.

100 All three enzymatic assays are based on the same observed phenomen

101 antitative RT-PCR (qRT-PCR) mRNA expression, enzymatic assay aromatase activity, scratch assay cell m

102 eptide ligand complex has been developed for enzymatic assays as an alternative to immuno-based detec

103 A new enzymatic assay, based on photometric detection of ammon

104 e down and increase the throughput of glycoT enzymatic assays because traditional methods require lar

105 slightly less active than 1a in the p38alpha enzymatic assay but displays a superior pharmacokinetic

106 of the reaction products, ADP, in a coupled enzymatic assay by continuous fluorimetric monitoring of

107 the HRP:sol-gel material was demonstrated by enzymatic assays by using I(-), guaiacol, and ABTS as su

108 Enzymatic assays, chemical proteomic profiling, kinome-w

109 he cage and Trp-containing hexapeptide under enzymatic assay conditions.

110 actions, spectroelectrochemical studies, and enzymatic assays conducted in the presence of DT-diaphor

111 Mutational and enzymatic assays confirm the importance of this lysine i

112 Enzymatic assays confirmed a difference in urease expres

113 Enzymatic assays confirmed its activity of beta-galactos

114 Furthermore, enzymatic assays confirmed that AK transforms galaxolide

115 Immunoblot and enzymatic assays confirmed that the increases in AKR1C m

116 In vitro enzymatic assays confirmed that the JmjC domain of Rph1

117 Enzymatic assays confirmed that the predicted membrane t

118 Significantly, enzymatic assays confirmed that this protein is necessar

119 A novel enzymatic assay coupled to formation of fluorescent chro

120 A series of enzymatic assays covering the multiplicity of functions

121 roach was taken featuring organic synthesis, enzymatic assaying, crystallography, and modeling includ

122 Enzymatic assays demonstrate that LpxJ and homologues in

123 Enzymatic assays demonstrate that mutants harboring the

124 Mass spectrometry-based lipidomics and enzymatic assays demonstrate the targeting mechanisms re

125 Direct enzymatic assays demonstrated a comparable reduction in

126 Indeed, enzymatic assays demonstrated that AtGA2ox7 and AtGA2ox8

127 In vitro enzymatic assays demonstrated that recombinant HasB2 is

128 Chemical and enzymatic assays demonstrated that the bacterium release

129 An enzymatic assay determined the impact of BAC over-expres

130 script and metabolite analyses together with enzymatic assays determined that the biosynthesis of ant

131 assay could also be used to complement a PTx-enzymatic assay, developed recently, and together they m

132 f inhibition of the HDAC activity in an HDAC enzymatic assay differed strikingly on their effects on

133 effect-directed assays, like bioassays or an enzymatic assay, directly linked with chromatography and

134 Moreover, unlike enzymatic assays (e.g., elastase), there is no confusion

135 onventional methods, including fluorescence, enzymatic assays (ELISA), and quantitative real-time PCR

136 An enzymatic assay established that 2a was an ATP-competiti

137 Finally, enzymatic assays established that both CmPP36 and Delta

138 sensitivity and 88% specificity, whereas the enzymatic assay exhibited 83% sensitivity and 80% specif

139 Imaging results were corroborated by enzymatic assays, flow cytometry, and histopathologic an

140 We have developed a continuous enzymatic assay for adenosine that facilitates high-thro

141 agents, making it suitable as a quantitative enzymatic assay for the activity of individual chitin sy

142 With the aim of developing a specific enzymatic assay for the detection and quantification of

143 sing 1D (31)P-NMR spectroscopy and a coupled enzymatic assay for the determination of inorganic phosp

144 A simple, rapid enzymatic assay for the determination of inorganic pyrop

145 We have developed quantitative enzymatic assays for AhpF alone and for the combined Ahp

146 Enzymatic assays for ATP and lactate production confirme

147 Enzymatic assays for catalase and dihydrolipoamide dehyd

148 med using immunoflourescence, AlphaLISA, and enzymatic assays for cathepsin B inhibition.

149 tions, two new simple and robust fluorogenic enzymatic assays for MGL and AHCY were developed and app

150 isolates from infected mice were screened by enzymatic assays for SpeB and the platelet-activating fa

151 The enzymatic assays for the above-mentioned metabolites all

152 usefulness of the microchips for performing enzymatic assays for which fluorogenic substrates cannot

153 nd stavudine (d4T) were determined, using an enzymatic assay, for 5 HTLV-1 isolates and for reference

154 Our enzymatic assays further reveal a robust regulatory mech

155 The PLP enzymatic assay has a lower limit of detection of 14.8 n

156 The enzymatic assay has also indicated the existence of an a

157 as 11-cis-retinol dehydrogenase (cRDH), and enzymatic assays have confirmed the isolation of an acti

158 In vitro cellular and enzymatic assays, however, demonstrate that leflunomide

159 Finally, enzymatic assays identified ceramide synthase as the mec

160 distinguished by a simple, highly sensitive enzymatic assay in cell lysates.

161 , while catalytic activity was determined by enzymatic assay in the presence (total activity) and the

162 mated as 15 monomer units per tetramer by an enzymatic assay in which polyphosphate kinase is used to

163 henazine reduction in Pseudomonas aeruginosa Enzymatic assays in cell-free lysate, together with crud

164 In vitro enzymatic assays in patient fibroblast cells showed defi

165 ophotometric experiments in combination with enzymatic assays in solution showed that TsdB acts as an

166 ratio) bilayers using (2)H-NMR and specific enzymatic assays in the absence or presence of 7.5 mol %

167 Gene expression and enzymatic assays indicate that erythroid 5-aminolevulini

168 Site-directed mutagenesis and enzymatic assays indicate that the interaction is fundam

169 ings, our biophysical experiments and kinase enzymatic assays indicate that the L834R mutation causes

170 m1 sequence was homologous to chitinase, and enzymatic assays indicated a 3-5-fold increase in chitin

171 o transcription-translation experiments, and enzymatic assays indicated that ORF3 encodes a product w

172 Enzymatic assays indicated that the (S)- and (R)-isomers

173 ompounds have been reported to inhibit IN in enzymatic assays, inhibition is not always correlated wi

174 An enzymatic assay, involving collection of a phenolic subs

175 nce a large number of commercially available enzymatic assay kits utilize NADH in their detection, th

176 n due to pre-absorption by the product of an enzymatic assay leads to quenching of the PL of quantum

177 Enzymatic assays led to the discovery that PA4872 decarb

178 to adefovir diphosphate (ADVDP) in in vitro enzymatic assays (<2.3-fold decreased sensitivity).

179 Enzymatic assays measured catalytic activity of cathepsi

180 rvested 4 days later for immunostaining, NOS enzymatic assay, measurement of cGMP, and vasomotor stud

181 However, using a direct enzymatic assay measuring cleavage of the synthetic pept

182 Immunoblotting, quantitative RT-PCR, enzymatic assays, nucleotide sugar, and sugar phosphate

183 croscopy and gel electrophoresis, as well as enzymatic assay of B(12)-dependent diol dehydratase.

184 on using in vivo bioluminescence imaging and enzymatic assay of lung homogenates.

185 Apoptosis was assessed by enzymatic assay of myocardial caspase 3 and TUNEL staini

186 This was confirmed by enzymatic assay of the partially purified enzyme with un

187 Enzymatic assay of these mutant alleles demonstrate that

188 However, enzymatic assay of total solubilized RNA polymerase I an

189 Its attributes are ideal for enzymatic assays of alkaline phosphatase for both basic

190 Enzymatic assays of both recombinant proteins showed try

191 t nervous system by using Northern blots and enzymatic assays of brain and subfractionated primary ne

192 Enzymatic assays of caa3 with these constructs, showed t

193 In addition, enzymatic assays of choline kinase activity in cells wer

194 Previous enzymatic assays of crude cell extracts indicated that a

195 Enzymatic assays of filtered culture supernatants reveal

196 gene expression at mRNA and protein levels, enzymatic assays of gene function, clonal analysis of PC

197 uenzae YbaK using site-directed mutagenesis, enzymatic assays of isosteric substrates and functional

198 1 encodes a pectin methylesterase (PME), and enzymatic assays of QRT1 expressed in Escherichia coli i

199 In addition, enzymatic assays of the wild-type proteins as well as se

200 Phenotypic and enzymatic assays on grafted plants indicated that mRNAs

201 esults from HPLC and MS analysis of in vitro enzymatic assays on stereochemically defined SP substrat

202 Enzymatic assays on TA0175 revealed that this enzyme cat

203 Enzymatic assays on the insect-cell-expressed human GALN

204 We used a combination of enzymatic assays, PA-binding assays, and circular dichro

205 us tissues for metabolism studies, including enzymatic assays, paper chromatography, and high-perform

206 Enzymatic assays performed under anaerobic conditions in

207 In the present study, direct enzymatic assays performed with alternative substrates a

208 Enzymatic assays performed with APC purified from strain

209 l toolbox that includes lipid substrates for enzymatic assays, potent inhibitors, and chemical probes

210 he standard technique, namely, a homogeneous enzymatic assay (R(2) = 0.97).

211 Enzymatic assays, reductive titrations, and circular dic

212 urrently available radioreceptor binding and enzymatic assays require extensive sample preparation an

213 ng and the resultant phenotypes evaluated by enzymatic assay, resistance to cytotoxic drugs, ability

214 LIS assay was completely consistent with the enzymatic assay results.

215 solution as a heat-stable homotetramer, and enzymatic assays reveal that the expressed enzyme is ful

216 A mass spectrometry based enzymatic assay revealed that two compounds, N(6)-benzyl

217 threonine are substrates for T. maritima TA, enzymatic assays revealed a strong preference for L-allo

218 nalysis of MJ0936 against a panel of general enzymatic assays revealed catalytic activity toward bis-

219 Western blots and arginase enzymatic assays revealed high-level expression of cytos

220 In vitro enzymatic assays revealed that ADP-ribose and NADH are p

221 Complex I enzymatic assays revealed that the 3460A mutation result

222 Enzymatic assays revealed that the distribution patterns

223 In vitro enzymatic assays revealed that the mutant enzymes had in

224 are expressed in BoHV-4 infected cells, but enzymatic assays revealed that the spliced form is not a

225 Enzymatic assays revealed vast differences in PTP cataly

226 Enzymatic assays show that this is the only PNPase activ

227 Enzymatic assays showed a basal level of phosphatase act

228 nding assays with active site inhibitors and enzymatic assays showed that Ab58 is a competitive inhib

229 Enzymatic assays showed that glutamine, a signal of nitr

230 Enzymatic assays showed that RH1 was an in vitro substra

231 Enzymatic assays showed that T-705 and T-1105 are poor s

232 In addition, the results of enzymatic assays showed that the level of GDH during ano

233 In vitro enzymatic assays showed that the purified S55F mutant en

234 in expressed in Escherichia coli followed by enzymatic assay, showed that this enzyme is active on C8

235 ing data from Western and Northern blots and enzymatic assays showing that both proteins are widely e

236 glucopeptides were synthesized and tested in enzymatic assays, showing a common minimal epitope.

237 Enzymatic assay shows the two fusions to have similar re

238 This is the first report of an enzymatic assay specific for FMN-bound NADPH cytochrome

239 Enzymatic assays, sucrose density gradient centrifugatio

240 ard the presence of inactive mutants than in enzymatic assays, suggesting a weak coupling of ATPase a

241 established and validated a straightforward enzymatic assay suitable for high-throughput screening a

242 The enzymatic assay targets alanine and employs alanine tran

243 Here we describe an enzymatic assay that allowed us to monitor Pmt4 activity

244 Also described is an array-based enzymatic assay that can be used to sequence portions of

245 The use of a drug-inducible enzymatic assay that depletes the Golgi-associated pool

246 Described in this study is an enzymatic assay that determines levels of homocysteine i

247 ions of that study were based on an indirect enzymatic assay that was not specific for (6-4) photopro

248 Here, we optimized two colorimetric-based enzymatic assays that detect the ureido moiety of the DH

249 ising molecular reagents for high-throughput enzymatic assays that successfully bridge the gap betwee

250 Using an in vitro enzymatic assay, the recombinant TPPE was able to proces

251 In in vitro enzymatic assays, the V555I mutation displayed the most

252 We have adapted a continuous enzymatic assay to a medium-throughput format and carrie

253 ic airway inflammation in asthma, we used an enzymatic assay to determine thrombin activity in bronch

254 attering (SAXS), chemical cross-linking, and enzymatic assays to enlarge our understanding of the int

255 cular dynamics simulations, mutagenesis, and enzymatic assays to explore the molecular basis of this

256 In this study, we use quantitative enzymatic assays to fully characterize C9Holo and a leuc

257 ion, fluorescence polarization and NMR-based enzymatic assays to investigate the impact of phosphomim

258 ship), medicinal chemistry, and experimental enzymatic assays to optimize a previously identified sma

259 microscopy, in combination with informative enzymatic assays, to correlate biochemical activity with

260 By use of a reliable enzymatic assay together with X-ray crystallography as g

261 nts, metabolite quantification, and in vitro enzymatic assays under both saturating and non-saturatin

262 leukemia (RBL-2H3) mast cells using specific enzymatic assays, urea-polyacrylamide gel electrophoresi

263 tem to determine glucose was evaluated by an enzymatic assay using glucose oxidase, horseradish perox

264 the presence of biopolymers was assessed by enzymatic assay using natural and synthetic substrates.

265 Enzymatic assays using 31P nuclear magnetic resonance sp

266 Enzymatic assays using purified protein showed that unli

267 Enzymatic assays using recombinant proteins and D-cyclos

268 terase activity, as is evident from in vitro enzymatic assays using the purified protein.

269 An enzymatic assay was developed to measure tetrahydrometha

270 ia as a source of the aggrecanase enzyme, an enzymatic assay was established employing purified aggre

271 An enzymatic assay was used to detect the activity distribu

272 one- and two-electron reduction in isolated enzymatic assays was studied, and their potential to gen

273 tion, ESI-MS/MS-based peptide sequencing and enzymatic assay we further demonstrate that human RBCs c

274 By using a GCAP2-specific antibody in enzymatic assays, we confirmed that GCAP1 but not GCAP2

275 Using affinity pull-down and in vitro enzymatic assays, we confirmed the potent antibacterial

276 Using surface plasmon resonance and enzymatic assays, we found that albicin binds and stabil

277 By using a combination of mitogenic and enzymatic assays, we found that phosphorylation of the a

278 Using in silico design followed by enzymatic assays, we show that Abz and EDDnp significant

279 ere harvested after 7 weeks and TDC and STR1 enzymatic assays were carried out.

280 qRT-PCR, Western blotting, and enzymatic assays were performed to confirm the regulatio

281 his immunodominant antigen in H. capsulatum, enzymatic assays were performed to determine whether H.

282 Degenerate PCR and enzymatic assays were used to assess the presence of but

283 This model system was used to establish, by enzymatic assays, Western and Northern analyses, histoch

284 accumulation of peroxides in HU solutions by enzymatic assays, which explains the toxicity, as both N

285 e a significant level of metal activation in enzymatic assays, which is inconsistent with the behavio

286 s with a reporter) or throughput inherent in enzymatic assays widely used for HTS.

287 pionamide (10a) inhibits PDHK in the primary enzymatic assay with an IC(50) of 13 +/- 1.5 nM, enhance

288 opanamide (14e) inhibits PDHK in the primary enzymatic assay with an IC(50) of 16 +/- 2 nM, enhances

289 nd to inhibit gamma-secretase in a cell-free enzymatic assay with an IC(50) of 2.1 microM, a value si

290 tivity of a series of N-acylhydrazones in an enzymatic assay with PA-Nter endonuclease, as well as in

291 pases 3, 6, 8, and 9, as measured both by an enzymatic assay with synthetic substrates and by detecti

292 Enzymatic assays with a recombinant soluble form of heps

293 Such coupling of enzymatic assays with electrophoretic separations on a m

294 Homology searches, as well as direct enzymatic assays with M. jannaschii, failed to reveal th

295 Enzymatic assays with purified components revealed a com

296 Subsequent enzymatic assays with the nonribosomal peptide synthetas

297 alone and their activities were validated by enzymatic assays with the recombinant proteins or by the

298 e been investigated using organic synthesis, enzymatic assays, X-ray crystallography, and thermodynam