ライフサイエンスコーパス: enzyme assay

1 l conversions of steroids as substrates (for enzyme assays).

2 where reagents were added for a fluorescent enzyme assay.

3 ese ligands was confirmed using a functional enzyme assay.

4 bility was assessed by using a mitochondrial enzyme assay.

5 enzyme activity over the course of a 20 min enzyme assay.

6 ation were characterized using a radioactive enzyme assay.

7 in recipients by in vivo imaging and direct enzyme assay.

8 values found using the conventional coupled-enzyme assay.

9 1, showed an IC(50) of 140 pM in a cell-free enzyme assay.

10 ned for inhibitory activity in a traditional enzyme assay.

11 d using a novel, continuous protease-coupled enzyme assay.

12 was investigated using an in vivo complex of enzyme assay.

13 og, using a continuous, thermostable coupled enzyme assay.

14 bining 2D-gel electrophoresis and an on-blot enzyme assay.

15 ing IC(50)s < 400 nM in a partially purified enzyme assay.

16 This was confirmed experimentally by enzyme assay.

17 vo [18F] gamma counting and thymidine kinase enzyme assay.

18 e of HSV1-sr39TK protein by thymidine kinase enzyme assay.

19 -LO activity was suggested by cell-free 5-LO enzyme assay.

20 nds of wholemeal bread, was also assessed by enzyme assay.

21 elomerase activity as measured by the direct enzyme assay.

22 ted cholesterol 27-hydroxylation by in vitro enzyme assay.

23 inetic parameters were measured by a coupled enzyme assay.

24 bserved between the two isomers in the GGDPS enzyme assay.

25 ed by reverse phase HPLC for use in in vitro enzyme assays.

26 2 (MC2082), were tested in both cellular and enzyme assays.

27 ol labeling, mass spectrometry, and targeted enzyme assays.

28 affected, and confirm this hypothesis using enzyme assays.

29 ivity over the related Jak family kinases in enzyme assays.

30 of an Escherichia coli Tyr auxotroph and by enzyme assays.

31 with shotgun metagenomics and extracellular enzyme assays.

32 ficities using novel mass spectrometry-based enzyme assays.

33 and must be generated as an integral part of enzyme assays.

34 und parallels its potency and selectivity in enzyme assays.

35 trated using a panel of purified recombinant enzyme assays.

36 oderate to high inhibition of IN in purified enzyme assays.

37 of NS5B by the triphosphates in the in vitro enzyme assays.

38 used substrate for glutathione S-transferase enzyme assays.

39 , Fe(2+), Ni(2+), and Zn(2+) in the in vitro enzyme assays.

40 mortem analysis using histologic staining or enzyme assays.

41 t proteins were monitored by SDS-PAGE and by enzyme assays.

42 n lysates are then prepared and submitted to enzyme assays.

43 phically, by UV/visible spectroscopy, and by enzyme assays.

44 deficient strain of Escherichia coli, and by enzyme assays.

45 stern blot analysis, and O-methyltransferase enzyme assays.

46 confer resistance to FR171456 in growth and enzyme assays.

47 r screening and lacks reproducibility of the enzyme assays.

48 ically more complex than that for the single-enzyme assays.

49 C-MS), nuclear magnetic resonance (NMR), and enzyme assays.

50 lished by in vitro testing using recombinant enzyme assays.

51 y desirable for enhancing the selectivity of enzyme assays.

52 essential in vivo but not revealed by simple enzyme assays.

53 exhibited 30% less GCS activity by in vitro enzyme assay (19.7 +/- 1.1 versus 27.4 +/- 2.3 pmol GC/h

54 In a cell-free enzyme assay, 21 showed a CDK2/cycE IC(50) = 48 nM and w

55 For development of a solid-phase enzyme assay, 4.0-mg samples of washed HA powder were ex

56 rein, a novel automatic and integrated micro-enzyme assay (AImuEA) platform was proposed based on a u

57 assay calibration did not interfere with the enzyme assay allowing both measurements to be performed

58 detected and quantified using a fluorogenic enzyme assay and a numerical model.

59 hibition of DNA gyrase as corroborated in an enzyme assay and by the inhibition of precursor thymidin

60 monstrate submicromolar activity both in the enzyme assay and in a (14)C-emulsion assay employing cho

61 Using an in vitro enzyme assay and LC-MS, methylofuran was identified in c

62 Enzyme assay and nuclear magnetic resonance analyses of

63 ound 1 displayed a TarO IC50 of 125 nM in an enzyme assay and possessed very high lipophilicity (clog

64 in (i) inhibited LF protease activity in an enzyme assay and protected macrophages against recombina

65 Using a fluorometric coupled enzyme assay and smooth muscle myosin light chain (MLC)

66 e is a useful tool for the alpha-glucosidase enzyme assay and will facilitate compound screening for

67 ave tested this hypothesis by using in vitro enzyme assays and a range of acyl-ACP, malonyl-ACP, and

68 L-Trp, the reactivation was demonstrated by enzyme assays and by various spectroscopic techniques.

69 Validation through enzyme assays and customized (13) C metabolite profiling

70 These results were observed in both in vitro enzyme assays and flow cytometric analyses of Chinese ha

71 Together with enzyme assays and HPLC analyses of pollen extracts from

72 Enzyme assays and IFN-beta reporter assays of cGAS mutan

73 Enzyme assays and immunoblot analyses indicated that 5-F

74 yltransferase I but not II both in cell-free enzyme assays and in transfected cells.

75 rase and sialyltransferase activity based on enzyme assays and microarray analysis.

76 ted by using the rapid (<5 min) amperometric enzyme assays and pH-sensitive iridium oxide films.

77 ombination of Sanger sequencing, restriction enzyme assays and targeted deep sequencing.

78 In vitro enzyme assays and transmission electron microscopy showe

79 Enzyme assays and Western blots show that whole heart ho

80 ed through in vitro binding assays, in vitro enzyme assay, and through functional cellular assays.

81 genes were identified by homology analysis, enzyme assay, and/or functional complementation.

82 ty of soluble form of CD73 using radioactive enzyme assays, and CD73 messenger RNA levels from leukoc

83 epancies, nuisance inhibition, sophisticated enzyme assays, and limited structural information about

84 stry, RNA interference, quantitative RT-PCR, enzyme assays, and lipidomic analyses of endocannabinoid

85 st of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were es

86 uently confirmed with reporter gene fusions, enzyme assays, and real-time PCR.

87 ctivity against the antibiotic cefotaxime in enzyme assays, and the mutant enzymes all lost thermodyn

88 Because the current enzyme assays are nonisoform specific, we examined assoc

89 his work, we introduce an entirely automated enzyme assay based on capillary electrophoresis coupled

90 ittle tolerance for substitution in purified enzyme assays, but a few analogues retained MIC, presuma

91 ces the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encap

92 The enzyme assay can be conducted in aqueous solution where

93 ncluded that kinetically based, stopped-flow enzyme assays can be performed in 60 s or less with a mi

94 ors of cPLA 2 alpha in a variety of isolated enzyme assays, cell based assays, and rat and human whol

95 t from the perfusion chip was pumped into an enzyme assay chip for monitoring of secretion from the c

96 In addition, enzyme assays, chlorophyll content and light microscopy

97 First, a coupled enzyme assay clearly demonstrated the ability of thiamin

98 An enzyme assay compatible with high-throughput screening (

99 targeted, functional ferrochelatase based on enzyme assays, complementation, and inhibitor studies.

100 round fluorescence and are more stable under enzyme assay conditions than phenolic substrates due to

101 Under standard enzyme assay conditions, the initial rate of FAS-indepen

102 ) is localized to the flagella, and in vitro enzyme assays confirm that the protein is a MAP kinase.

103 A colorimetric enzyme assay confirmed these findings.

104 Metabolic labeling and in vitro enzyme assays confirmed direct inhibition of the cytosol

105 Enzyme assays confirmed that these residues are absolute

106 In vitro enzyme assays confirmed that VTE3 is the plant functiona

107 Enzyme assays confirmed the presence of PME, PAE, and PL

108 dopsis enzyme (AtFMN/FHy) increased when the enzyme assays contained 0.02% Tween 20.

109 In a coupled enzyme assay containing GTR and wild-type GSAT, addition

110 make the ICECEA competitive with the optical enzyme assays currently in use.

111 ctural data was then used to rationalize the enzyme assay data.

112 hanges in mitochondrial proteins, and direct enzyme assay demonstrated changes in both complexes I an

113 In vitro enzyme assays demonstrated that both cell types can hydr

114 E. coli, and the use of FACE as an in vitro enzyme assay detected possible substrate preferences for

115 demonstrate that the cell-based assay and GO enzyme assay developed in this study are useful for furt

116 he presence of other histone peptides in the enzyme assay, enabling investigation of cross-reactivity

117 Equally small values in an RdpA enzyme assay (epsilonea = -1.0 +/- 0.1 per thousand) and

118 ces of this interaction were studied through enzyme assay experiments, where DAO enzymatic activity w

119 For fluorometric enzyme assay (FA) 1 (FA-1), 2'-(4-methylumbelliferyl)-al

120 An enzyme assay for DAPK was developed and used to measure

121 port the development of a continuous coupled enzyme assay for FDPS activity that involves the subsequ

122 ers, we were also able to establish a robust enzyme assay for measuring bsNOS activity and inhibition

123 ovel, thermostable adaptation of the coupled-enzyme assay for monitoring glucose concentrations was d

124 A coupled-enzyme assay for MshC was developed using pyrophosphatas

125 A microchip-based enzyme assay for protein kinase A is described.

126 ed IC(50)s consistent with those measured by enzyme assay for the activated form of biotin-p38alpha.

127 es were synthesized and tested in a p38alpha enzyme assay for their inhibition of tumor necrosis fact

128 ta analysis have allowed us to develop basic enzyme assays for substrate specificity and inhibitor ac

129 Enzyme assays further confirmed the identities of both N

130 The on-line enzyme assay had a limit of detection (LOD) of 4 microM

131 acylation and signaling assays as no direct enzyme assay had yet been developed.

132 To date, no enzyme assay has been developed for the Ddi1 proteins, b

133 Enzyme assays have revealed that the farnesyl pyrophosph

134 inhibitor of anaplastic lymphoma kinase (ALK enzyme assay IC(50) = 0.174 muM) during high throughput

135 rnally calibrated electrochemical continuous enzyme assay (ICECEA) has proved to work well for single

136 rnally calibrated electrochemical continuous enzyme assay (ICECEA).

137 rnally calibrated electrochemical continuous enzyme assay (ICECEA, patent pending) was developed for

138 Enzyme assays, immunoblotting, immunohistochemical testi

139 mical characteristic of lipid I renders MurX enzyme assays impractical for screening and lacks reprod

140 me-free preassay calibration with the actual enzyme assay in one continuous experiment.

141 An enzyme assay in the recombinant RB51 strains indicated t

142 Enzyme assays in presence of protease inhibitors indicat

143 of the work was to perform kinetically based enzyme assays in the stopped-flow mode using a system of

144 No luciferase activity was detected by enzyme assays in tissue homogenates of BM recipients, an

145 d number of candidates were characterized by enzyme assays in vitro, gene expression analysis, non-po

146 t reduced the activity of partially purified enzymes assayed in a reconstituted assay system.

147 studies of interactions with ARF6, in vitro enzyme assays, in vivo toxicity assays, and in vivo proc

148 tus Bath, along with NADH and duroquinol, to enzyme assays increased the activity of purified prepara

149 Enzyme assays indeed confirmed an electron transferase a

150 The in vitro enzyme assays indicate that these distinct metabolic phe

151 es complementation experiments, and in vitro enzyme assays indicate that this P450 is a ferulic acid/

152 Enzyme assays indicated both CGL and CBS enzyme activity

153 ene sequence, reverse transcriptase PCR, and enzyme assays indicated that dntD dntE ORF13 dntG compos

154 Enzyme assays indicated that Gpm did not require Mn(2+)

155 In vitro enzyme assays indicated that its preferred substrate is

156 Enzyme assays indicated that the stalk mutations resulte

157 We describe the development of an enzyme assay inside picoliter microdroplets.

158 ounds were tested in several serine protease enzyme assays involved in the coagulation cascade exhibi

159 epared and tested in several serine protease enzyme assays involved in the coagulation cascade.

160 Following extraction, enzyme assays involving recombinant farnesyl protein tra

161 e absence of an independent thymidine kinase-enzyme assay is discussed.

162 sition of the assay solution for the coupled-enzyme assays is typically more complex than that for th

163 valuation of these analogs in a human MetAP2 enzyme assay led to the identification of several inhibi

164 ided fractionation using signal transduction enzyme assays led to the isolation of the novel spiroket

165 ays, the behavior of a drug in a biochemical enzyme assay may not accurately reflect its performance

166 with a specific activity of 200 Ci/mmol for enzyme assays, metabolic studies, and tissue imaging.

167 A coupled-enzyme assay method based on the chemiluminescent detect

168 The method provides a simple and reliable enzyme assay method that enables the rapid diagnosis of

169 e-based assay substrates and a novel coupled-enzyme assay method.

170 We have used an in vitro enzyme assay, monitoring hdm2-catalyzed Ub transfer from

171 Using enzyme assays, NMR, mutagenesis, and deletion of kdgF, w

172 Coupled enzyme assays of AMP and pyrophosphate release in the re

173 Enzyme assays of atrial tissue homogenates confirmed inc

174 One explanation that emerged from enzyme assays of deoxycytidine kinase (dCK) and deoxycyt

175 Map-based cloning of the KNF gene and enzyme assays of knf embryos demonstrated that KNF encod

176 Enzyme assays of MUR4 protein expressed in the methylotr

177 Enzyme assays of pancreatic triglyceride lipase (PTL) sh

178 Enzyme assays of recombinant protein supported the hypot

179 were validated by Northern blot analysis and enzyme assays of selected genes.

180 mmunocytochemistry and/or immunoblotting and enzyme assays of subcellular fractions.

181 Real-time PCR and PI-PLC enzyme assays of the TCS mutants, coupled with SrrA prom

182 Enzyme assays of wild-type embryos showed a rapid rise i

183 Enzyme assays on 17 isolates of borreliae demonstrated G

184 Enzyme assays on a series of substrates confirm that bot

185 Enzyme assays on cell extracts localized total UDP-D-glu

186 on-modification (R-M) tests, direct in vitro enzyme assays or more recently from bacterial genome seq

187 Experimental data from continuous enzyme assays or protein folding experiments often conta

188 peptides for other purposes, including other enzyme assays or protein-binding ligands.

189 chemical studies like protein purifications, enzyme assays, organelle isolation or determination of m

190 alizarin red S stain for mineralization, and enzyme assay (p-nitrophenol phosphate cleavage) for alka

191 gamma in the native cells were compared with enzyme assays performed in vitro.

192 e secreted amylase activity as determined by enzyme assay, plate assay, or Western blot analysis.

193 Two-step pore-limit electrophoresis with enzyme assay (PLENZ) is conducted in a single, straight

194 chemistry, immunoblotting, and a fluorogenic enzyme assay presenilin-1 (required for gamma-secretase

195 o imaging analysis (R2=0.98) and by in vitro enzyme assay (R2=0.94).

196 -stat has the potential for applications for enzyme assays, reagentless pH control, acidity/alkalinit

197 was detected by modifying the chip to allow enzyme assay reagents to be mixed with dialysate as samp

198 Conventional radioactive PARP enzyme assay requires the separation of the polymer prod

199 orogenic substrate for the alpha-glucosidase enzyme assay, resorufin alpha-d-glucopyranoside.

200 ium were 0.096, 0.108, and 2.3 muM in the GO enzyme assay, respectively.

201 ve immunocytochemistry (ICC), and 5-[(3)H]FC enzyme assay, respectively.

202 sured by surface plasmon resonance (SPR) and enzyme assays, respectively.

203 On the basis of enzyme assay results, three mutants were identified for

204 Heterologous expression and enzyme assay reveal that 3 of these alleles encode sulfa

205 imulation, flow cytometry and a cell surface enzyme assay reveal that the C-terminal catalytic domain

206 Enzyme assays revealed that gli1 lacks glycerol kinase a

207 Enzyme assays revealed that Orf6 has a higher specific a

208 cs, real time reverse transcription-PCR, and enzyme assays revealed that the expression levels of som

209 e presence of a hydrolase (GXSXG) motif, and enzyme assays revealed the presence of monoacylglycerol

210 d-type or LPB-Tag prostates as determined by enzyme assay, reverse transcription-PCR, and immunohisto

211 a combination of mass spectrometry, RNA-seq, enzyme assays, RNAi and phylogenomics in different non-m

212 These lines were then assessed by detailed enzyme assay, RT-qPCR and fruiting.

213 mer interface, thereby destabilizing and, as enzyme assays show, inactivating the enzyme.

214 Enzyme assays showed a corresponding overabundance of th

215 In vitro enzyme assays showed a significant increase in forward P

216 (2) In vitro enzyme assays showed potent inhibition of JAK3- and Syk-

217 Cell-based enzyme assays showed selective inhibition of JAK1/3-depe

218 Enzyme assays showed significant 11 beta-HSD2 activity (

219 Accordingly, enzyme assays showed that 3-nitrotyramine and its oxidat

220 Enzyme assays showed that ATP was the preferred substrat

221 Enzyme assays showed that cell extracts from a pduL muta

222 The enzyme assays showed that CNT nearly doubled the retenti

223 Enzyme assays showed that expression strains produced 87

224 Enzyme assays showed that one of the adenylyltransferase

225 Although early in vitro enzyme assays showed that recombinant BAR and PAT exhibi

226 -4.4-fold increase of radioactivity, whereas enzyme assay shows 22.1+/-6.1-fold increase of thymidine

227 In contrast to results with individual enzyme assays, specificity for thioredoxin f or m was no

228 ions of the LDLR class A repeats by in vitro enzyme assays suggested a major role of GalNAc-T11.

229 Enzyme assays supported the structure-based predictions

230 hosphate, together with in vivo and in vitro enzyme assays, supports a pyridoxal phosphate-dependent

231 This paper describes a microfabricated enzyme assay system including a micromixer that can be u

232 This enzyme assay takes advantage of the amplification charac

233 in silicone oil was measured using a linked enzyme assay that coupled ADP production to the oxidatio

234 an in vitro, single-well, fluorescence-based enzyme assay that monitors the first step of the PAT rea

235 e Erf2.Erf4 PAT, we have developed a coupled enzyme assay that monitors the turnover of the palmitoyl

236 Both had muscle mitochondrial enzyme assays that showed a pronounced mitochondrial hyp

237 nst clinically relevant HIV-1 mutants and in enzyme assays, the simultaneous C5(methyl)/C6(methyl/eth

238 (S)-enantiomer was detected using a coupled enzyme assay; The rate of elimination of HF from the (R)

239 it 5-lipoxygenase catalysis in a broken cell enzyme assay; therefore it is likely that 47.Na acts as

240 activity in a continuous fluorescence-based enzyme assay, this protease variant allowed the determin

241 rved association, using a custom restriction-enzyme assay to analyze the DNA in 158 samples from 29 p

242 .2 chemically and subjected it to an on-blot enzyme assay to characterize the activity.

243 use a coupled SWI-SNF remodeling-restriction enzyme assay to directly compare the remodeling of monon

244 s, immunohistochemistry, H&E and biochemical enzyme assays to determine the role of Src kinase on mit

245 electron paramagnetic resonance, and coupled enzyme assays to investigate how single mutations at pos

246 These protocols require from 1 d (enzyme assays) to up to 3 months (autoradiography of [(3

247 In enzyme assays, Trx1 activated two selected targets follo

248 Common enzyme assays use optical methods that often require the

249 The enzyme assay used here might find wider application in s

250 on rate and the sensitivity of low-abundance enzyme assay using a micro/nanofluidic preconcentration

251 f a rapid, simple, and robust LC-MS/MS-based enzyme assay using dried blood spots (DBS) for the diagn

252 We also developed a GO enzyme assay using the hydrogen peroxide-Amplex red repo

253 bstrate preference as determined by in vitro enzyme assays using 9-/13-hydroperoxy linolenic and 9-/1

254 In addition, in vitro enzyme assays using both (32)P- and (14)C-radiolabeled s

255 shown for select diTPSs, single and coupled enzyme assays using microbial and plant expression syste

256 Data from in vitro enzyme assays using microsomes showed that preexposure o

257 g each of the nine cDNAs were active in LACS enzyme assays using oleic acid as a substrate.

258 Enzyme assays using the sugar donor beta-d-arabinofurano

259 d through optimization of successive coupled enzyme assays using UDP-n-acetylglucosamine as the initi

260 Cell-free enzyme assays, using the crude Ayg1p protein extract, re

261 We present a deuterium-label based enzyme assay, utilizing a series of peptide substrates w

262 Enzyme assays validated the increase in isocitrate lyase

263 d adipocytes with on-line fluorescence-based enzyme assay was developed to monitor glycerol secretion

264 A sensitive fluorescent enzyme assay was used to quantify expression levels for

265 A deuterium-label-based coupled-enzyme assay was used to rapidly determine the stereoche

266 A colorimetric enzyme assay was used to screen for oxalate oxidase acti

267 channels designed for rapid electrophoretic enzyme assays was developed.

268 Linearity of the enzyme assays was seen for both time (0-16 min) and cyto

269 Using an HPLC-based MalQ enzyme assay, we could demonstrate that the equilibrium

270 Using an in vitro enzyme assay, we determined that all four proteins are M

271 s of Pseudomonas aeruginosa, and an in vitro enzyme assay, we have identified both PMM and PGM activi

272 Using reconstituted enzyme assays, we also demonstrated that sertraline inhi

273 ochemistry, western blotting, and functional enzyme assays, we assessed the distribution, quantity, a

274 Through microscopy and enzyme assays, we demonstrated the particles were able t

275 se of domain deletions, binding studies, and enzyme assays, we find that the WD40 repeats confer a sa

276 Using in vitro enzyme assays, we show that the C-terminal tail portion

277 ral information with sequence alignments and enzyme assays, we were able to elucidate determinants of

278 approximately 10-fold improved potency in an enzyme assay were identified, and this improved activity

279 e ICECEA in the development of other coupled-enzyme assays were also discussed.

280 Enzyme assays were also performed with IP as an indicato

281 ass spectrometry (MS) data were supported by enzyme assays, Western blot, and histochemistry.

282 utilization or depletion of carbon sources, enzyme assays, Western blotting and mass spectrometric a

283 over 70% inhibition activity at 50 nM in the enzyme assay, whereas the corresponding C-4 regioisomers

284 method for a universal fluorescence turn-on enzyme assay, which relies on the target enzyme-triggere

285 polymerase chain reaction-based restriction enzyme assay with mismatched primers was employed to sho

286 Phytol feeding and enzyme assays with Arabidopsis and recombinant Escherich

287 concordance existed among Q-RT-PCR, ICC and enzyme assays with both cell lines.

288 In vitro enzyme assays with candidate genes from the region of th

289 hibiting fungal endo-polygalacturonases, but enzyme assays with extracts of AS-SHY pollen do not supp

290 In coupled enzyme assays with purified PFOR and FldA, FqrB reduced

291 Heterologous expression and in vitro enzyme assays with purified RPHs from diverse bacterial

292 e polyprenol-deficient yeast rer2 mutant and enzyme assays with recombinant AtCPT7 confirmed that the

293 In vitro enzyme assays with recombinant CrNCED1 protein showed th

294 ynthase to the epithelium of glands and used enzyme assays with recombinant protein of the same gene

295 omplementation tests in Escherichia coli and enzyme assays with recombinant proteins confirmed that A

296 and retina isomerases were then measured by enzyme assays with specific enzyme inhibitors.

297 th Escherichia coli mutants, by the in vitro enzyme assays with the recombinant proteins, and by the

298 Using metabolite analysis, enzyme assays with WRC tissue extracts, cloning, and fun

299 d shown to inhibit BoNT/A LC in a FRET-based enzyme assay, with confirmation in an HPLC-based assay.

300 Using enzyme assays, X-ray crystal structures, and simulations