ライフサイエンスコーパス: enzymology

1 nts a fundamental challenge in assembly line enzymology.

2 cusing on their biochemistry, structure, and enzymology.

3 prominent position in the field of helicase enzymology.

4 identified that parallel those encounterd in enzymology.

5 ues to drive advances in protein science and enzymology.

6 tG) presents a new challenge in heme protein enzymology.

7 irst proposal of microenvironment effects in enzymology.

8 wer has been one of the challenges of modern enzymology.

9 , metabolite screening, complementation, and enzymology.

10 uctures, have led to real insight into their enzymology.

11 polyketide synthase and fatty acid synthase enzymology.

12 interaction mechanism in NRPS assembly line enzymology.

13 hydrogen-transfer reactions in chemistry and enzymology.

14 is one of the major challenges in nonaqueous enzymology.

15 is unprecedented in ADP-ribosyl transferase enzymology.

16 abolism and have been paradigms of classical enzymology.

17 ive-site with only modest affect on myosin's enzymology.

18 general use in cell biology, metabolism, and enzymology.

19 ature and opens new avenues in chemistry and enzymology.

20 enabling new approaches to study Integrator enzymology.

21 tions has been a fundamental focus in modern enzymology.

22 matics, computational biology, and molecular enzymology.

23 tigate fundamental and practical problems in enzymology.

24 ading domain in the context of assembly-line enzymology.

25 chemical bonds is an evolving perspective in enzymology.

26 molecular evolution, molecular biology, and enzymology.

27 to elucidate the N,C-bipyrrole homocoupling enzymology.

28 s an exciting and challenging area of modern enzymology.

29 nsic reaction-cycle time of about 0.4 s from enzymology.

30 tions has been a fundamental focus in modern enzymology.

31 To investigate DNA replication enzymology across the nuclear genome of budding yeast, d

32 The results of steady-state enzymology analysis were consistent with allostery and s

33 To improve our understanding of the enzymology and activation mechanism of Syk, we character

34 ht into analogous behavior in biophysics and enzymology and aims to inform the design of efficient se

35 regulated modifications that alter PKCdelta enzymology and allow for stimulus-specific control of ca

36 More generally, comparative enzymology and analysis of catalytic promiscuity can pro

37 While the enzymology and biological consequences have been extensi

38 entified and characterized, both in terms of enzymology and biological function.

39 Using a combination of genomics, enzymology and crystallography, we show that the mucin-d

40 enzymes is a fundamental challenge for both enzymology and drug design.

41 ed for high-throughput screening methods for enzymology and drug discovery.

42 Based on a combination of enzymology and gene-analysis, a new degradative pathway

43 Although the enzymology and genetics of PHB metabolism in S. meliloti

44 Here we describe the crystal structure, enzymology and in vitro activity on human substrates of

45 adical chemistry common to both coenzyme B12 enzymology and its known photochemistry.

46 A description of the enzymology and mechanism of terpenoid cyclization is fol

47 entury witnessed many advances in scientific enzymology and microbiology that laid the foundations fo

48 Our results illustrate how classical enzymology and modern protein design can each inform the

49 Progress in enzymology and molecular biology of SA biosynthesis and

50 The enzymology and molecular genetics of biosynthesis of pht

51 een directly demonstrated by single molecule enzymology and NMR studies recently.

52 ntly discovered peptides, review advances in enzymology and protein engineering, and discuss the regu

53 important information for understanding the enzymology and regulation of L- and S-SMase and for expl

54 rve as an excellent model for studies of the enzymology and regulation of this protein.

55 n an enzyme very similar to wild type in its enzymology and secondary structure, indicating that this

56 sults expand our understanding of telomerase enzymology and should encourage the development of paras

57 tudies of lambda biology, lambda exonuclease enzymology and the availability of the X-ray crystallogr

58 sing knowledge about nuclear DNA replication enzymology and the rate and specificity with which misma

59 reflections on these studies that began with enzymology and the use of cultured mammalian cells, and

60 ion of the newly generated FMS constructs by enzymology and thermoshift assays demonstrated similar a

61 s advanced our knowledge of isoform-specific enzymology and will facilitate the discovery of novel PI

62 Distinct functions shape distinct enzymologies, and this is illustrated by comparing kines

63 familiar arithmetic concepts from classical enzymology, and also about connections between modeling

64 ompelling problem in fundamental mechanistic enzymology, and as part of our mechanistic studies of th

65 e the advantages of heterogeneous catalysis, enzymology, and molecular catalysis represents the next

66 -molecule catalysis and synthetic chemistry, enzymology, and molecular evolution to discover or creat

67 he challenges in protein design, mechanistic enzymology, and molecular evolution.

68 rotein-protein interaction assays, G protein enzymology, and morphological analysis, we demonstrate h

69 combination of virology, molecular biology, enzymology, and protein structural and modeling studies

70 a combination of computational predictions, enzymology, and structural biology.

71 Using a large-scale "genomic enzymology" approach, we (i) assigned novel ATP-dependen

72 which limits the utility of well-established enzymology approaches developed under the assumption of

73 Biochemical, super-resolution imaging, and enzymology approaches establish that this Gravin scaffol

74 ation of cell biology, chemical biology, and enzymology approaches to analyze this processing event.

75 etazoa raises the prospect of an alternative enzymology based on covalently activated 3' ends.

76 ta-reductase is a unique reaction in steroid enzymology because hydride transfer from NADPH to the be

77 t have challenged fundamental assumptions in enzymology, biochemistry and cell metabolism regarding t

78 terdisciplinary fields such as biochemistry, enzymology, biofuels, bioengineering and drug discovery.

79 ylation is an ancient process with conserved enzymology but diverse biological functions that include

80 pping is an important mechanism in molecular enzymology, but its study is limited by the lack of an a

81 Biologists working in topological enzymology can use this program to compute and visualize

82 ly pose fascinating questions of metabolism, enzymology, cell biology, and regulation; the answers ha

83 unprecedented view of lactate dehydrogenase enzymology, confirming the timescale of substrate-induce

84 e histology, contractile protein content and enzymology contribute to exercise intolerance in CHF.

85 ructure-based drug design (SBDD), synthesis, enzymology data, and X-ray crystallography results are d

86 lustrate the application of ABPs to protease enzymology, discovery and development of protease-target

87 that pseudomonads utilize sulfite reduction enzymology distinct from that of E. coli and possibly si

88 a powerful tool for protein engineering and enzymology, drug discovery, and proteomics.

89 r, KIEs have not come into widespread use in enzymology, due in large part to the requirement for pro

90 the practical development and application of enzymology, e.g., in the interpretation and prediction o

91 Sequence alignments, enzymology experiments and inhibitor studies on p300/CBP

92 n was overexpressed in Escherichia coli, and enzymology experiments demonstrated its unsaturated beta

93 Enzymology experiments show that the MDDEF performs a se

94 Bulk enzymology experiments verify that the enzyme is noncomp

95 The predictions were tested by enzymology experiments, and Arad3529 deaminated many pte

96 netics of this molecular motor to see if its enzymology explains this remarkable degree of processivi

97 sights into how these two motors adapt their enzymologies for their distinct functions.

98 nd untapped potential of targeting bacterial enzymology for improvements in human health.

99 ich may have applications in many aspects of enzymology, from the design of novel enzymes to the pred

100 Entry into lignan enzymology has been gained by the approximately 3000-fol

101 Membrane protein enzymology has been hampered by the inherent insolubilit

102 Single-molecule enzymology has come a long way since the early demonstra

103 Radical S-adenosylmethionine (RS) enzymology has emerged as a major biochemical strategy f

104 In contrast, bipyrrole-coupling enzymology has not been observed.

105 The study of membrane protein structure and enzymology has traditionally been hampered by the inhere

106 A cherished tenet of nucleic acid enzymology holds that synthesis of polynucleotide 3'-5'

107 me function and demonstrates single-particle enzymology in a system of more than two components.

108 e-binding C2 domain that controls PKCdelta's enzymology indirectly by decreasing phosphorylation in t

109 In classical enzymology, intermediates and transition states in a cat

110 ribed to the acetamide group and the unusual enzymology involved, we enzymatically interrogated diver

111 even less has been established regarding the enzymology involved.

112 A significant contemporary question in enzymology involves the role of protein dynamics and hyd

113 uncover a new catalytic motif in radical SAM enzymology involving the use of two 5'-deoxyadenosyl rad

114 Crystallography and enzymology involving WT and Ala190 uPA were used to calc

115 Computational enzymology is a rapidly maturing field that is increasin

116 defense, an understanding of the underlying enzymology is lacking.

117 evidence for their catalytic significance in enzymology is lacking.

118 The main focus of enzymology is on the enzyme rates, substrate structures,

119 s particularly applicable to single-molecule enzymology is presented.

120 A substantial challenge for genomic enzymology is the reliable annotation for proteins of un

121 A central goal of enzymology is to understand the physicochemical mechanis

122 e of the most intriguing questions in modern enzymology is whether enzyme dynamics evolved to enhance

123 ty has grown from many directions: genetics, enzymology, kinetics, physical biochemistry, and thermod

124 This is achieved by adopting an enzymology-like framework, where transfer functions are

125 approach of substrate heat pre-treatment and enzymology may be used to influence proteolysis with att

126 ure determination, computation, experimental enzymology, microbiology, and structure-based annotation

127 nation of stereoselective organic synthesis, enzymology, microbiology, and X-ray crystallography was

128 position of this internal clock by combining enzymology, molecular biology, genetics, and modeling ap

129 ding paradigm in modular polyketide synthase enzymology, namely the definition of a module, has been

130 plications as diverse as structural biology, enzymology, nanotechnology and systems biology.

131 The enzymology of 135 assembly lines containing primarily ci

132 biosynthesis, the recycling of NADH, and the enzymology of 2-hydroxyacid dehydrogenases are discussed

133 We now report the synthesis, structure, and enzymology of a set of 20 smSNAREs (small molecule SNAr-

134 amplification of RNA targets is based on the enzymology of a single thermostable DNA polymerase and t

135 ss in the understanding of the chemistry and enzymology of abasic DNA largely relies upon the study o

136 dation for quantitatively characterizing the enzymology of any number of clinically relevant LPLAT pr

137 The enzymology of bacterial lignin breakdown is currently no

138 However, little is known about the enzymology of BER of altered in-tandem CpG dinucleotides

139 The enzymology of CD39L2 shows characteristic features of a

140 sary forerunner to defining the genetics and enzymology of cell wall polymer-peptidoglycan ligation i

141 In efforts to understand the complex enzymology of CYP17, structure/function relationships ha

142 tida KT2440 to elaborate on the genomics and enzymology of d-amino acid metabolism.

143 the role of a flavin reductase, DszD, in the enzymology of desulfurization, and to the use of new too

144 cellular dysfunction and tissue damage, the enzymology of FAEE metabolism remains poorly understood.

145 To better understand the enzymology of hemoglobin degradation, it is necessary to

146 solve a long-standing riddle about the basic enzymology of IDE with important implications for the et

147 ith other nuclear hormone receptors, yet the enzymology of its physiological generation remains uncle

148 While the enzymology of lambda-terminase has been well described,

149 ubstituents is necessary for elucidating the enzymology of lipid A biosynthesis and for characterizin

150 However, little is known about the enzymology of mitochondrial O-GlcNAcylation.

151 d level of genetic complexity underlying the enzymology of N-terminal myristoylation and suggest that

152 To date, the polarity and enzymology of NMD in mammalian cells is unknown.

153 To study the enzymology of OGT, independent of potential regulatory p

154 However, the biological functions and enzymology of p68 RNA helicase are not well characterize

155 The cellular functions, regulation and enzymology of phosphatidylinositol (PtdIns) 5-P, the new

156 f PKS domains that expands the chemistry and enzymology of PKSs and might be exploited to incorporate

157 The emphasis in this review is on the enzymology of prenyl protein processing and the function

158 loration of intein structure and the unusual enzymology of protein splicing.

159 In this study, the enzymology of replication past site-specific 1,N(6)-gamm

160 The enzymology of RNase YI*, a recently discovered endoribon

161 n major advances in our understanding of the enzymology of sirtuins, their regulation, and their abil

162 lable and their mechanistic details, (e) the enzymology of some of the key reactions required for rep

163 neous fluorescence-based method to study the enzymology of specialized DNA polymerases in real time.

164 chain dehydrogenases/reductases and into the enzymology of steroid and retinoid metabolism.

165 Understanding the enzymology of such enzymes should provide the informatio

166 ral approaches are now shedding light on the enzymology of the APC.

167 an important gap in our understanding of the enzymology of the assembly of coenzyme B(12).

168 The genetics and enzymology of the biosynthesis of wall teichoic acid hav

169 past few years, much knowledge of the basic enzymology of the Cdc25 phosphatases that may aid in the

170 Although the enzymology of the DD-carboxypeptidases has been studied

171 versify the linear peptide both to probe the enzymology of the macrocyclizing enzyme, TycC thioestera

172 spired many total synthesis efforts, but the enzymology of the okaramine biosynthetic pathway remains

173 ts, but much remains to be learned about the enzymology of their formation.

174 ned and opens the way to direct study of the enzymology of this complex biosynthetic pathway.

175 In addition, we examine the basic enzymology of this enzyme through molecular modeling stu

176 r location of Ii degradation, as well as the enzymology of this event, are important in determining t

177 To study the enzymology of this interesting process we have expressed

178 lmost nothing is known about the function or enzymology of this modification.

179 Understanding the enzymology of this pathway will be key to success for th

180 Recent advances in the enzymology of transcription and chromatin regulation hav

181 In the present study we have examined the enzymology of ubiquitination of endogenous InsP3Rs in mu

182 l utility in our global understanding of the enzymology of virus assembly.

183 The enzymology of VLDL triglyceride synthesis is still poorl

184 tensively studied, little is known about the enzymology of volatile aldoxime formation.

185 nds for NTE inhibition and investigating the enzymology of wild-type and mutant forms of NTE.

186 Detailed enzymology on this enzyme has been limited, due to diffi

187 ck, novel approaches based on new chemistry, enzymology or next generation sequencing have emerged.

188 Little is known about the enzymology or regulation of Mg-chelatase, as it has been

189 es in fields as varied as organic chemistry, enzymology, or protein folding.

190 recent advancements in characterizing their enzymology, our knowledge about the dynamic processes of

191 We present enzymology, pathway organization, and evolutionary analy

192 Single molecule enzymology provides an opportunity to examine details of

193 Single-molecule enzymology provides an unprecedented level of detail abo

194 nd energetic analysis, fluorescence studies, enzymology, pulse proteolysis, X-ray crystallography, an

195 omerase, fundamental questions regarding its enzymology remain unanswered.

196 major issue for both structural genomics and enzymology research.

197 Enzymology revealed that the Psd2(C2Delta)p and Psd2(GRD

198 Here, by combining enzymology, structural biology, and activity-based metab

199 t understanding of the microbial physiology, enzymology, structural biology, and folding of the proka

200 Recent advances in enzymology, structural biology, and protein chemistry ha

201 utagenesis of the triad residues followed by enzymology studies of mutant and wild-type hBATs.

202 onstrate the utility of AMPL for mechanistic enzymology studies, an (18)O-labeled substrate was gener

203 o understand the changes in biochemistry and enzymology that accompanied adaptation to O2, we integra

204 logical opportunities afforded by nonaqueous enzymology, the issue of often drastically diminished en

205 structure and regulation, protein structure, enzymology, tissue distribution and pathophysiological r

206 , and computational tools brings mechanistic enzymology to a new era, one poised for novel fundamenta

207 as been accompanied by a shift of focus from enzymology to a search for substrates as well as the fir

208 e the last frontier in protein chemistry and enzymology to be conquered.

209 m natural products discovery and mechanistic enzymology to elucidate the structure and biosynthesis o

210 lecular dynamics simulations, synthesis, and enzymology to explore potential pharmacophoric features

211 avior requires specific adaptations of motor enzymology to meet these unique physiologic demands.

212 nt form due to the application of industrial enzymology to solve process related problems.

213 Our work suggests use of the nanopore as an enzymology tool and provides a means to identify single

214 clude multiple hosts and expression systems, enzymology tools for in vitro study, and ways to enginee

215 Despite great interest in CYP enzymology, two in vitro aspects of CYP3A4 catalysis are

216 stingly, aminomalonyl-ACP formation involves enzymology typically found in nonribosomal peptide synth

217 ies have contributed to our knowledge of the enzymology underlying acetylation, including efforts to

218 ps and suggests some interesting features of enzymology using a surface-bound substrate.

219 Based on RNase H enzymology, we enhanced single nucleotide discrimination

220 r to the kinase by x-ray crystallography and enzymology while demonstrating potency, selectivity, and

221 Here, in crystallo enzymology with the catalytically active bacterial cellu

222 a mechanism distinct from ubiquitylation in enzymology yet analogous to ubiquitylation in targeting