ライフサイエンスコーパス: epimerase

1 effected by the enzyme mutarotase (aldose-1-epimerase).

2 ase; OSBS), and epimerization (L-Ala-D/L-Glu epimerase).

3 d chain processing (sulfotransferases and an epimerase).

4 esis of HS involves sulfotransferases and an epimerase.

5 oupling system can be used to assay for C(5)-epimerase.

6 e structural and functional analysis of C(5)-epimerase.

7 ling assay to determine the activity of C(5)-epimerase.

8 does not function as a UDP-GlcNAc/UDP-GalNAc epimerase.

9 homology in Escherichia coli UDP-galactose 4-epimerase.

10 ne (RP 333) product designated as a putative epimerase.

11 that TviB is a dehydrogenase and TviC is an epimerase.

12 ues in a reaction catalyzed by C5-mannuronan epimerase.

13 ei and is initiated by the enzyme UDP-Glc 4'-epimerase.

14 diated by AlgG, a periplasmic C-5 mannuronan epimerase.

15 that share similarity to both reductase and epimerase.

16 the restricted substrate specificity of this epimerase.

17 on and galE, which encodes the UDP-glucose-4-epimerase.

18 ommodated within the active site of tyvelose epimerase.

19 ly predicted to encode a UDP-glucuronic acid epimerase.

20 % homology to the human D-glucuronic acid C5-epimerase.

21 e existence of a prokaryotic D-glucuronyl C5-epimerase.

22 targeting the dimeric antibiotic target DAP epimerase.

23 transferases, HS sulfotransferases, and C(5)-epimerase.

24 ate binding residues in homologous bacterial epimerases.

25 his study to elucidate the function of these epimerases.

26 tein with sequence similarity to UDP-D-Glc 4-epimerases.

27 al agents against this family of bacterial 2-epimerases.

28 The main IdoA-producing enzyme, DS epimerase 1 (DS-epi1), together with the 6-O- and 4-O-su

29 Other conserved motifs of the epimerases, 361-NNRSYEN and 381-NLVAYN, are predicted to

30 35, next in the pathway, is a bifunctional 3-epimerase 4-reductase.

31 ase (GMDS) and GDP-4-keto-6-deoxymannose 3,5-epimerase-4-reductase (FX or tissue specific transplanta

32 ase (GMDS) and GDP-4-keto-6-deoxymannose 3,5-epimerase-4-reductase (FX or tissue specific transplanta

33 ice lacking the GDP-4-keto-6-deoxymannose3,5-epimerase-4-reductase enzyme (FX knockout) exhibited sig

34 a bifunctional UDP-4-keto-6-deoxyglucose-3,5-epimerase/-4-reductase that converts UDP-4-keto-6-deoxyg

35 VbsL is a PLP-dependent epimerase acting at C(2) of the 10 atom monomer unit.

36 Unexpectedly, all three epimerase-active DH domains were also found to possess i

37 Four redox-inactive mutants of epimerase-active EryKR1 were engineered by mutagenesis o

38 of short chain dehydrogenase-reductases, the epimerase-active KR(0) domains from polyether synthases

39 ose, and (iii) both UDP-Gal and UDP-GalNAc 4-epimerase activities in cell extracts.

40 s all had both dihydroneopterin aldolase and epimerase activities, and carried out the aldol cleavage

41 increased Gne/Mnk protein expression and Gne-epimerase activities.

42 and UDP-N-acetylgalactosamine (UDP-GalNAc) 4-epimerase activities.

43 cNAc-5,6-ene 4-oxidase, 5,6-reductase, and 5-epimerase activities.

44 ies, whereas its DdahB homologue only had C3 epimerase activity along its cognate pathway.

45 Thus, AlgG is bifunctional with (i) epimerase activity and (ii) a role in protecting alginat

46 Gne cDNA had restored in vitro UDP-GlcNAc 2-epimerase activity and cell surface PSA expression.

47 t mutations in this motif disrupt mannuronan epimerase activity but have no effect on alginate secret

48 resultant loss of feedback inhibition of GNE-epimerase activity by CMP-sialic acid causes excessive p

49 Feedback inhibition of GNE-epimerase activity by CMP-sialic acid recovered after si

50 One mutation (S306Y) resulted in a loss of epimerase activity for non-acetylated substrates by abou

51 To characterize the epimerase activity of AlgG further, the algG4 allele of

52 anoyl-ACP (7a), consistent with the proposed epimerase activity of each of the KR(0) domains.

53 dules Epimerase assay revealed the intrinsic epimerase activity of NanDH1 and NanDH5, from modules 1

54 These results establish the intrinsic epimerase activity of redox-inactive KR(0) domains, rule

55 ese EryKR1(0) mutants retained the intrinsic epimerase activity of the parent EryKR1 domain.

56 An epimerase activity previously proposed to function on sp

57 Lec3 mutants with no UDP-GlcNAc 2-epimerase activity represent sensitive hosts for charact

58 ated that lpsL encoded a UDP-glucuronic acid epimerase activity that was reduced in the lps-212 mutan

59 At P2, significantly decreased Gne-epimerase activity was observed in Gne(M712T/M712T) musc

60 n the bacterial enzyme resulted in a loss of epimerase activity with regard to UDP-Gal by almost 5-fo

61 y, Lec3 cells had no detectable UDP-GlcNAc 2-epimerase activity, and Lec3 cells grown in serum-free m

62 ketoreductase domains also have an intrinsic epimerase activity, thus enabling mechanistic analysis o

63 ngmycin synthase, were shown to have cryptic epimerase activity.

64 B. anthracis Sterne designations), exhibited epimerase activity.

65 G, which revealed a critical residue for C-5-epimerase activity.

66 ovel mechanism for inactivating UDP-GlcNAc 2-epimerase activity.

67 spores in mother cells required UDP-GlcNAc 2-epimerase activity.

68 ned UDP-Gal 4-epimerase but not UDP-GalNAc 4-epimerase activity.

69 n of a bacterial enzyme with D-glucuronyl C5-epimerase activity.

70 rtions, indicating that both enzymes possess epimerase activity.

71 first evidence for bacterial D-glucuronyl C5-epimerase activity.

72 back into cysteine regained 3-hydroxyproline epimerase activity.

73 d three recombinant KR domains with putative epimerase activity: NysKR1 from module 1 of the nystatin

74 G mutant of the monofunctional l-Ala-d/l-Glu epimerase (AEE) from Escherichia coli catalyzed a low le

75 ution, the D297G mutant of the l-Ala-d/l-Glu epimerase (AEE) from Escherichia coli was designed so th

76 hesized reversibly by a novel GlcNAc-P-P-Und epimerase after the formation of GlcNAc-P-P-Und by WecA

77 ecreted and calcium-dependent mannuronan C-5 epimerases (AlgE1-7).

78 rison of AlgG and the extracellular alginate epimerase AlgE4 of Azotobacter vinelandii provides a str

79 re converted to alpha-L-guluronate by the C5-epimerase AlgG to produce a polymer of alternating beta-

80 ructural studies of d-allulose 6-phosphate 3-epimerase (ALSE) from Escherichia coli K-12 that catalyz

81 unction, were discovered to be L-Ala-D/L-Glu epimerases, although they also catalyze the epimerizatio

82 niscent to that observed for UDP-galactose 4-epimerase, an enzyme that plays a key role in galactose

83 that a single plant enzyme has both the 3,5-epimerase and 4-keto reductase activities.

84 tified in bacteria and shown to encode a 3,5-epimerase and a 4-keto reductase that together convert d

85 sion of a Pseudomonas aeruginosa Glc(NAc) C4-epimerase and a human polypeptide GalNAc-transferase in

86 the functional UDP-N-acetyl-D-glucosamine-2-epimerase and CsaB the functional poly-ManNAc-1-phosphat

87 nd cytosolic localization of the Arabidopsis epimerase and dehydratase.

88 complexity for assaying the activity of C(5)-epimerase and facilitate the structural and functional a

89 UDP-GlcNAc 2-epimerase and GlcNAc 2-epimerase are two enzymes capable

90 ymatic activities, UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase, in sialic acid

91 extracts localized total UDP-D-glucuronate 4-epimerase and recombinant GAE1 activity exclusively to t

92 eneral experimental basis for decoupling the epimerase and reductase activities of a large class of P

93 ct prokaryotic PLP-dependent isopenicillin N epimerase and the fungal isopenicillin N epimerase two p

94 receptor may be disease-relevant targets in epimerase and transferase galactosemias, and identify UG

95 istidine-tagged protein, shows UDP-GlcNAcA 4-epimerase and UDP-N-acetylgalactosamine (UDP-GalNAc) 4-e

96 nd Cjj1427 are the only members of the C3/C5 epimerases and C3/C5 epimerase/C4 reductase families sho

97 htforward with two distinct types (racemases/epimerases and cis-trans isomerases), but reactions enta

98 t belongs to the 'RED' family of reductases, epimerases and dehydrogenases.

99 This precursor gave rise to sugar epimerases and metal-binding sugar isomerases.

100 t crystal structures of cofactor-independent epimerases and racemases, cocrystallized with substrates

101 mically characterized members that are sugar epimerases and/or reductases.

102 array of specialized glycosyl transferases, epimerase, and sulfotransferases, this approach should m

103 g glycosyltransferases, heparan sulfate C(5)-epimerase, and sulfotransferases.

104 such as glycogen phosphorylase, UDP-GlcNAc 2-epimerase, and the glycosyl transferase MurG.

105 ng phosphoglucomutase (pgm), UDP-galactose-4-epimerase, and two other NTHI sialyltransferases (lic3A

106 UDP-GlcNAc 2-epimerase and GlcNAc 2-epimerase are two enzymes capable of generating ManNAc f

107 In contrast to other enzymes, most epimerases are only active on sugars substituted with ph

108 These epimerases are responsible for the epimerization of beta

109 ay, galactose mutarotase and UDP-galactose 4-epimerase, are contained within a single polypeptide cha

110 e (EIX) and a newly developed Tandem Modules Epimerase assay revealed the intrinsic epimerase activit

111 In an in vitro UDP-GlcNAc 2-epimerase assay, Lec3 cells had no detectable UDP-GlcNAc

112 is described here demonstrates that tyvelose epimerase belongs to the short-chain dehydrogenase/reduc

113 le work were cyclophilin A and UDP-glucose-4-epimerase, both of which are known to interact with CsA,

114 onal GalE1 but not GalE2 contained UDP-Gal 4-epimerase but not UDP-GalNAc 4-epimerase activity.

115 C(5)-epimerase (C(5)-epi) is a key enzyme in this pathway.

116 e putative C3/C5 epimerase Cjj1430 and C3/C5 epimerase/C4 reductase Cjj1427 from the capsular cluster

117 ly members of the C3/C5 epimerases and C3/C5 epimerase/C4 reductase families shown to have activity o

118 between AlgE4 and AlgE6 resulted in a novel epimerase called AlgE64 with increased G-block forming a

119 omotes biosynthesis of sialic acid, GlcNAc 2-epimerase can serve a catabolic role, diverting metaboli

120 nd, suggesting that strain O157 contained an epimerase capable of interconverting GlcNAc-P-P-Und and

121 annuronate residue to l-guluronate along the epimerase catalytic face.

122 C5-mannuronan epimerase catalyzes the formation of alpha-L-guluronate

123 UDP-galactose 4-epimerase catalyzes the interconversion of UDP-Gal and U

124 Tyvelose epimerase catalyzes the last step in the biosynthesis of

125 product, an ADP-L-glycerol-D-mannoheptose-6-epimerase, catalyzes the conversion of ADP-D-glycerol-D-

126 curonate; however, no coding regions for the epimerase catalyzing this reaction have previously been

127 abidopsis, mutation of RHD1, a UDP-glucose-4-epimerase, causes root-specific phenotypes, including hy

128 Cellobiose 2-epimerase (CE) reversibly converts d-glucose residues in

129 Herein, we characterized the putative C3/C5 epimerase Cjj1430 and C3/C5 epimerase/C4 reductase Cjj14

130 The HS C(5)-epimerase converts glucuronic acid to iduronic acid.

131 Incubating N-sulfated heparosan with C(5)-epimerase converts some of the glucuronic acid to iduron

132 y and/or biochemically intermediate cases of epimerase deficiency have also been reported.

133 5%-64% of control levels, demonstrating that epimerase deficiency is not a binary condition; it is a

134 s, consistent with a diagnosis of peripheral epimerase deficiency, many did not.

135 period, received the diagnosis of hemolysate epimerase deficiency.

136 Epimerase-deficiency galactosemia results from the impai

137 Defects in human GALE result in the disorder epimerase-deficiency galactosemia.

138 a potential novel therapy for patients with epimerase-deficiency galactosemia.

139 However, cDNAs encoding the known epimerase-deficient mutation H132A or the new Lec3 G135E

140 coding sugar methylases and nucleotide sugar epimerase-dehydratase proteins.

141 monooxygenase, and a possible NAD-dependent epimerase/dehydratase.

142 ic domain found in a large protein family of epimerase/dehydratases.

143 /reductase (SDR), belonging to the NDP-sugar epimerases/dehydratases subclass.

144 often annotated incorrectly as NAD-dependent epimerases/dehydratases; therefore, their prevalence in

145 meable, small molecule inhibitor of GlcNAc 2-epimerase designed based on mechanistic principles.

146 We show that Cjj1430 serves as C3 epimerase devoid of C5 epimerization activity and that C

147 A X-ray crystal structure of the E. coli DAP epimerase dimer shows for the first time that the enzyme

148 The epimerases display a modular structure composed of one o

149 cterial species, P. aeruginosa C5-mannuronan epimerase does not require Ca2+ for activity, and the Ca

150 The epimerase domain of PchE, involved in pyochelin biosynth

151 Nor are any of the canonical epimerase domains of nonribosomal peptide synthetase (NR

152 We suggest these inserts are noncanonical epimerase domains, reversibly deprotonating and reproton

153 tified decaprenylphosphoryl-beta-d-ribose 2'-epimerase (DprE1) as the primary target responsible for

154 to be decaprenylphosphoryl-beta-D-ribose-2'-epimerase (DprE1).

155 ion of decaprenylphosphoryl-beta-d-ribose-2'-epimerase (DprE1).

156 inhibit decaprenylphosphoryl-beta-D-ribose2'-epimerase (DprE1).

157 ion of sugar nucleotides, like UDP-galactose epimerase, dTDP-glucose-4,6-dehydratase, and UDP-xylose

158 ArnA resembles UDP-galactose epimerase, dTDP-glucose-4,6-dehydratase, and UDP-xylose

159 tyrocidine have D-amino acids, introduced by epimerase (E) domains embedded within modules of the enz

160 e chain growth from L-Phe residues by 50 kDa epimerase (E) domains embedded, respectively, in the ini

161 ii 38 depends on the dual specificity of the epimerase encoded by galE2.

162 , this extension does not seem to hinder the epimerase enzymatic active site.

163 However, the epimerase enzymatic activity of GNE3 and GNE8 is likely

164 Deletion of this fragment likely eliminates epimerase enzymatic activity.

165 UDP-GlcNAc 2-epimerase enzymes have been shown to be required for the

166 AS5117) encode nearly identical UDP-GlcNAc 2-epimerase enzymes that catalyze the reversible conversio

167 Furthermore, ADP-hep 6-epimerase exhibited a preference for binding of NADP(+)

168 Previous studies report that DAP epimerase exists as a monomeric enzyme.

169 Here we study the effects of GlcNAc 2-epimerase expression on sialic acid production in cells.

170 The WbmF active site contains conserved 3,5-epimerase features, namely, a positionally conserved cys

171 omplex of L-Ala-L-Glu with the L-Ala-D/L-Glu epimerase from Bacillus subtilis is reported.

172 (8)-barrel domains in both the l-Ala-d/l-Glu epimerase from Escherichia coli (AEE) and the muconate l

173 graphy, and enzyme kinetic analyses that DAP epimerase from Escherichia coli exists as a functional d

174 x-ray crystallographic analysis of tyvelose epimerase from Salmonella typhi complexed with CDP.

175 ity of the purified recombinant tagaturonate epimerase from T. maritima was directly confirmed and ki

176 those of UDP-N-acetylglucosamine (GlcNAc) 2-epimerases from both prokaryotes and eukaryotes.

177 Consequently, 3,5-epimerase function can probably be ruled out for these e

178 s shown to encode a protein lacking only the epimerase function.

179 d functionally express a UDP-D-glucuronate 4-epimerase (GAE1) from Arabidopsis.

180 ucuronic acid by the action of glucuronate 4-epimerases (GAEs).

181 chanistically, we identified UDP-galactose-4-epimerase (GalE) as a direct transcriptional target of X

182 The enzyme UDP-galactose 4'-epimerase (GALE) catalyses the reversible epimerisation

183 UDP-galactose 4'-epimerase (GALE) catalyzes the final step in the Leloir

184 UDP-galactose 4'-epimerase (GALE) catalyzes the final step of the highly

185 UDP-galactose 4'-epimerase (GALE) catalyzes the interconversion of UDP-ga

186 UDP-galactose 4'-epimerase (GALE) interconverts UDP-galactose and UDP-glu

187 ults from the impairment of UDP-galactose 4'-epimerase (GALE), the third enzyme in the Leloir pathway

188 II results from the loss of UDP-galactose 4'-epimerase (GALE), which interconverts UDP-galactose and

189 tain an intact gene encoding a UDP-galactose epimerase (galE1) and a truncated remnant (galE2), respe

190 ollowing CBI, activation of a UDP-D-xylose 4-epimerase gene correlated with increases in arabinose an

191 Deletion of the nanE epimerase gene or the rokA hexokinase gene, whose produc

192 ive1 (rhd1) lacks a functional UDP-glucose 4-epimerase gene, UGE4, which is involved in channeling UD

193 biosynthesis, three annotated UDP-glucose 4-epimerase genes of B. anthracis were cloned and expresse

194 xpression of Robo1, Robo2, and glucuronyl C5-epimerase (GLCE), and that an intact miR-218-Slit-Robo r

195 Recombinant maize and Arabidopsis epimerases (GRMZM2G061988, At5g49970) rapidly interconve

196 The recombinant epimerase has a predicted molecular mass of approximatel

197 ng three HS-modifying enzymes, glucuronyl C5-epimerase, heparan 6O-sulfotransferase, and 2O-sulfotran

198 Plants have homologs of both enzymes, the epimerase homolog being fused to the vitamin B6 salvage

199 Our genetic analyses show that the HS C-5 epimerase hse-5, the HS 2-O-sulfotransferase hst-2, or t

200 hesis of heparan sulfate (HS), glucuronyl C5-epimerase (Hsepi) catalyzes C5-epimerization of glucuron

201 tion of: (i) a new group of presumed Ala-Glu epimerases; (ii) several enzymes with specificity for hy

202 to the mechanism of galactose sensitivity in epimerase-impaired cells and suggest a potential novel t

203 of NADH dehydrogenase and methylmalonyl-CoA epimerase improved PA tolerance.

204 Uge5 is the dominant UDP-glucose 4-epimerase in A. fumigatus and is essential for normal gr

205 dl-D cells defective in UDP-Gal/UDP-GalNAc 4-epimerase in which N- and O-linked glycosylation can be

206 This study is the first survey of glucose epimerases in A. fumigatus and contributes to our unders

207 o be functionally characterized were Ala-Glu epimerases in Eschericiha coli and Bacillus subtilis, ba

208 osphate isomerase and ribulose 5-phosphate 3-epimerase) in the pentose phosphate pathway were overexp

209 hydroxypentanoyl-ACP (6a) with redox-active, epimerase-inactive EryKR6 from module 6 of the 6-deoxyer

210 hose of other characterized UDP-GlcNAc/Glc 4-epimerases indicated that it has relaxed specificity tow

211 owing: The 4-13C chemical shift in wild-type epimerase is 149.9 ppm; mutation of Ser 124 to Ala chang

212 Here, we show that the parasite epimerase is a homodimer that can interconvert UDP-Glc a

213 Accordingly, DAP epimerase is a promising antimicrobial target.

214 The epimerase is encoded by the TbGALE gene and procyclic fo

215 thus demonstrating that dimerization of DAP epimerase is essential for catalysis.

216 Diaminopimelate (DAP) epimerase is involved in the biosynthesis of meso-DAP an

217 The epimerase is required for glycoprotein galactosylation.

218 Mg534 is a 4,6-dehydratase 5-epimerase; its three-dimensional structure suggests that

219 l-Gal-1-phosphate phosphatase, GDP-Man-3',5'-epimerase, l-Gal dehydrogenase, and l-galactono-1,4-lact

220 , as did a truncated form of the Arabidopsis epimerase lacking the pyridoxine 5'-phosphate oxidase do

221 ample of haploid insufficiency suggests that epimerase levels are close to limiting in this life cycl

222 Roles for UDP-GlcNAc 2-epimerase/ManNAc 6-kinase (GNE) beyond controlling flux

223 The genes for UDP-GlcNAc-2-epimerase/ManNAc kinase (EK), sialic acid 9-phosphate sy

224 UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE) catalyzes the first two co

225 GNE (UDP-GlcNAc 2-epimerase/ManNAc kinase) myopathy is a rare muscle disor

226 Coexpression of SAS and UDP-GlcNAc 2-epimerase/ManNAc kinase, the bifunctional enzyme initiat

227 me of sialic acid biosynthesis, UDP-GlcNAc 2-epimerase/ManNAc kinase.

228 man ManNAc kinase domain of the UDP-GlcNAc-2-epimerase/ManNAc kinase.

229 tion of the bifunctional enzyme UDP-GlcNAc-2-epimerase/ManNAc kinase.

230 Only a Gne cDNA encoding UDP-GlcNAc 2-epimerase:ManNAc kinase rescued PSA synthesis.

231 r dynamics simulations indicate that the DAP epimerase monomer is inherently more flexible than the d

232 th coincided with constitutive re-expression epimerase mRNA.

233 to determine the activities of various C(5)-epimerase mutants.

234 Aldose-1-epimerase (mutarotase) catalyzes the interconversion of

235 sis, uridine diphospho-N-acetylglucosamine 2-epimerase/N-acetylmannosamine (ManNAc) kinase (GNE/MNK),

236 se (MNK) domain of UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase in complexes with M

237 s the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase that transforms UDP

238 Using UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase-deficient cells, we

239 lic acid biosynthesis, N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase.

240 essential N-acetylmannosamine-6-phosphate 2-epimerase (NanE) belongs to a convergent glycolytic path

241 ated GDP-6-deoxy-D-manno-heptose, but the C3 epimerase necessary to form GDP-6-deoxy-D-altro-heptose

242 e), nanK (ManNAc kinase), nanE (ManNAc-6-P 2-epimerase), neuS (polysialyltransferase) and neuB (siala

243 g a stereospecific dehydratase (NNRD) and an epimerase (NNRE), the latter being fused to a vitamin B6

244 among AlgG and the extracellular mannuronan epimerases of Azotobacter vinelandii.

245 hosphate-N-acetylglucosamine (UDP-GlcNAc) C4 epimerase, only the second microbial enzyme characterize

246 he activities of homologous nucleotide sugar epimerases or dehydratases.

247 KO of two isoforms of GDP-D-mannose epimerase (OsGME) reduced the foliar AsA level by 20-30%

248 on of the catabolic pathway in which Hyp-B 2-epimerase participates.

249 Unlike diaminopimelate epimerase, PhzF is a dimer in solution.

250 t to encode the UDP-N-acetyl-D-glucosamine-2-epimerase, poly-ManNAc-1-phosphate-transferase, and O-ac

251 Homology modeling with UDP-galactose-4-epimerase predicts that Asp36 is responsible for the NAD

252 er to form the aldolase products, 24% to the epimerase product and 25% to the oxygenase products.

253 se products were previously misidentified as epimerase products.

254 inding proteins than to other bacterial NanE epimerase proteins.

255 elates to the amount of the activity of C(5)-epimerase, proving that this two-enzyme coupling system

256 One key feature of the epimerase reaction mechanism is the rotation of a 4-keto

257 composition of the polymeric product of the epimerase reaction was analyzed by 1H NMR spectroscopy,

258 The reversibility of the epimerase reaction was demonstrated by showing that [3H]

259 rate of NanE, while ATP is a cofactor in the epimerase reaction.

260 345) neutralizes the acidic group during the epimerase reaction.

261 To identify the gene encoding the epimerase required to produce GalNAc for BclA oligosacch

262 ts role as the PLP-dependent nocardicin C-9' epimerase responsible for interconversion of the nocardi

263 ructural studies of d-ribulose 5-phosphate 3-epimerase (RPE) from Streptococcus pyogenes that catalyz

264 ssays determined that ribulose-5-phosphate 3-epimerase (Rpe) was specifically inactivated.

265 uperfamily includes d-ribulose 5-phosphate 3-epimerase (RPE), orotidine 5'-monophosphate decarboxylas

266 All plant NAD(P)HX dehydratase and epimerase sequences examined had predicted organellar ta

267 e lysine biosynthetic enzyme diaminopimelate epimerase, sharing an unusual fold consisting of two nea

268 In addition to the Ala-Phe epimerase specificity reported previously, we describe t

269 ology observed for all other UDP-galactose 4-epimerases studied thus far.

270 UDP-galactose 4'-epimerase (TbGalE), an enzyme of the Leloir pathway of g

271 These data suggest that neuC encodes an epimerase that catalyzes the formation of ManNAc from UD

272 AlgG is a periplasmic C-5-epimerase that converts poly d-mannuronate to the mixed

273 n to be the preferred substrate for TunF--an epimerase that converts the glucose derivative to a gala

274 that converts (S)-NAD(P)HX to NAD(P)H and an epimerase that facilitates interconversion of the R and

275 product encodes an UDP-N-acetylglucosamine 2-epimerase that generates ManNAc directly from the dinucl

276 In contrast to C5-mannuronan epimerases that have been characterized in other bacteri

277 zyme (rmlC; TDP-4-keto-6-deoxy-d-glucose 3,5-epimerase), the ATP binding cassette (ABC) sugar transpo

278 rates, in the reaction catalyzed by tyvelose epimerase, the inversion of stereochemistry occurs at C-

279 ew class of beta-lactam aminoacyl side chain epimerases, the first two classes being the evolutionari

280 The susceptibility of the C(5)-epimerase-treated N-sulfated heparosan to 2OST Y94I modi

281 n N epimerase and the fungal isopenicillin N epimerase two protein system.

282 erium SleL appears to be associated with the epimerase-type activity observed previously in B. subtil

283 three genes encoding putative UDP-glucose 4-epimerases, uge3, uge4, and uge5.

284 UDP-GlcA 4-epimerase (UGlcAE) catalyzes the epimerization of UDP-al

285 ne racemase superfamily, 4R-hydroxyproline 2-epimerase (UniProt ID A0NXQ7 ; 4HypE) and trans-3-hydrox

286 The presence of a novel epimerase was demonstrated by showing that exogenous [3H

287 ed that the additional activity in the human epimerase was due to replacement of the structural equiv

288 The epimerase was localized to the glycosomes by immunofluor

289 nsferase (galU), a UDP-N-acetylglucosamine 2-epimerase (wecB) and a UDP-N-acetyl-d-mannosamine dehydr

290 Two genes for putative galactose 4-epimerases were identified.

291 Unlike UDP-galactose 4-epimerase where the conversion of configuration occurs a

292 tor, has identified the regions of ADP-hep 6-epimerase, which defines its specificity for NADP(+).

293 folX encodes dihydroneopterin triphosphate epimerase, which interconverts dihydroneopterin triphosp

294 terized mutants defective in UDP-galactose 4-epimerase, which produced a defective lipopolysaccharide

295 r results indicate that, unlike UDP-GlcNAc 2-epimerase, which promotes biosynthesis of sialic acid, G

296 of ADP-hep 6-epimerase with UDP-galactose 4-epimerase, which utilizes an NAD(+) cofactor, has identi

297 codes for a manNAc/N-acetylglucosamine (NAG) epimerase, which, intriguingly, possesses more similarit

298 ility of a functional recombinant UDP-GlcA 4-epimerase will be of considerable value for the facile g

299 The specificity of the epimerase with regard to neighboring residues was examin

300 A structural comparison of ADP-hep 6-epimerase with UDP-galactose 4-epimerase, which utilizes