ライフサイエンスコーパス: error-prone PCR

1 Second, a mutant library is generated by error-prone PCR.

2 sons were made with a library constructed by error-prone PCR.

3 ing sites and introduced random mutations by error-prone PCR.

4 riosus (Pfu-Pol), with superb performance in error-prone PCR.

5 major segment of which had been amplified by error-prone PCR.

6 brary of amide synthetase mutants created by error-prone PCR.

7 Furthermore, the error-prone PCR alone introduced the mutation with high

8 the human coding region were synthesized by error-prone PCR and cloned directly in yeast by in vivo

9 wo methods of DNA manipulation are analysed: error-prone PCR and DNA recombination.

10 , to generate such mutants, we have utilized error-prone PCR and fluorescence lifetime imaging to scr

11 ient method of mutagenesis that makes use of error-prone PCR and homologous recombination to generate

12 Five rounds of error-prone PCR and iterative screening were performed w

13 Using directed evolution techniques of error-prone PCR and mutant library screening, a variant

14 a high-throughput screening method utilizing error-prone PCR and next-generation sequencing to compre

15 of a novel technique that incorporates both error-prone PCR and recombineering.

16 Three rounds of error-prone PCR and selection identified a treble mutant

17 A single round of error-prone PCR and selection yielded variant ALR(Y274F)

18 Error-prone PCR and site-directed mutagenesis led to the

19 enopus ribosomal protein L5 was generated by error-prone PCR and used to delineate the binding domain

20 DNA sequence variations were generated with error-prone PCR and were inserted in the promoter region

21 the highest-affinity aptamers evolved using error-prone PCR, and 27- or 46-fold higher affinities th

22 so identified after mutagenesis of FXYD2b by error-prone PCR coupled with a selection for cell prolif

23 yield higher-performing variants faster than error-prone PCR-derived libraries.

24 brary containing scFv mutants was created by error-prone PCR, displayed on the surface of yeast, and

25 Large libraries of hGSTT1-1 constructed by error-prone PCR, DNA shuffling, or saturation mutagenesi

26 enesis library, including those produced via error-prone PCR (ep-PCR), mutator Escherichia coli strai

27 Escherichia coli lsr operon promoters using error-prone PCR (ePCR) and selected for promoters that p

28 Error-prone PCR in realistic reaction conditions is pred

29 When mutations are enhanced through error-prone PCR, in vitro M2-seq experimentally resolves

30 Perhaps, the most popular method is the error-prone PCR, in which mistakes are introduced into a

31 Error-prone PCR is a DNA replication process that intent

32 method is further corroborated by assembling error-prone PCR libraries and regenerating templates fol

33 nalyzing four different replicative systems, error-prone PCR, mouse antibodies, a nematode, and Droso

34 variants identified during a single round of error-prone PCR mutagenesis and screening.

35 er improvements were facilitated by targeted error-prone PCR mutagenesis of loop-7, and additional PT

36 such mutants by a new approach that utilizes error-prone PCR mutagenesis, overlap extension PCR, and

37 Our findings demonstrate how optimal error-prone PCR mutation rates may be calculated, and in

38 od for random mutagenesis of cloned genes by error-prone PCR or DNA shuffling that eliminates the nee

39 ends observed among libraries constructed by error-prone PCR, preservation of function was observed t

40 Here, we show experimentally that error-prone PCR produces a broader non-Poisson distribut

41 First, the AAV2 cap gene is amplified in an error-prone PCR reaction and further diversified through

42 A collection of point mutations generated by error-prone PCR revealed two small regions required for

43 uorescence-based screen, in conjunction with error-prone PCR/saturation mutagenesis, for altering the

44 h this inhibitor, we randomly mutagenized by error-prone PCR the E. coli dsbB gene and selected dsbB

45 ed on structure-function considerations, and error-prone PCR to create random mutations.

46 We compared the results of using NRR and error-prone PCR to evolve DNA aptamers that bind strepta

47 Here, we used error-prone PCR to mutagenize the full-length human TS c

48 The mutant Pfu-Pols can be applied in error-prone PCR, under exactly the same conditions used

49 Error-prone PCR was combined with in vivo functional sel

50 f mutation frequency on affinity maturation, error-prone PCR was used to generate libraries containin

51 1) efficient in vitro cDNA mutagenesis (here error-prone PCR was used), 2) efficient retroviral deliv

52 By using error-prone PCR, we have isolated and characterized thre

53 olution, using active-site randomization and error-prone PCR, yielded a MetRS variant (designated Pra