ライフサイエンスコーパス: essential gene

1 ns are lethal, demonstrating that TAF1 is an essential gene.

2 with Cdc15 preceding Imp2, only cdc15 is an essential gene.

3 ells and larvae, demonstrating that it is an essential gene.

4 lori ADD is encoded by hp0267, an apparently essential gene.

5 without abolishing the endogenous role of an essential gene.

6 exploiting the ts mutation for studying the essential gene.

7 PARG is encoded by a single, essential gene.

8 pupal lethality, indicating that MCO1 is an essential gene.

9 mcb1(+) is an essential gene.

10 lic repair by HR is lethal due to loss of an essential gene.

11 e M. tuberculosis orthologue, Rv0227c, is an essential gene.

12 ng sgRNA sequences targeting a collection of essential genes.

13 123 instances of AGR codons removed from all essential genes.

14 ified 352 general and 199 condition-specific essential genes.

15 stringent regulation of multiple replication-essential genes.

16 ct Oryza sativa and Saccharomyces cerevisiae essential genes.

17 ortant tool in investigation of functions of essential genes.

18 re growth defects due to the inactivation of essential genes.

19 nserved than either non-essential domains or essential genes.

20 , CDC37, as one of the top six GIST-specific essential genes.

21 usual C-rich 3' splice sites in thousands of essential genes.

22 X chromosome and significantly enriched for essential genes.

23 s) mutations are important reagents to study essential genes.

24 3,362 bp and 1,012,010 bp), and both encoded essential genes.

25 9ac levels and deactivates the expression of essential genes.

26 use chromosome 11 contains a large number of essential genes.

27 ppressors, whereas GO genes are enriched for essential genes.

28 stable genetic elements normally not seen in essential genes.

29 cted to be rare since drug targets are often essential genes.

30 cid sequence and even allows modification of essential genes.

31 ll fate by upregulating expression of T-cell essential genes.

32 transgenic inducible RNAi for suppression of essential genes.

33 he specificity of CRIPSR screens to identify essential genes.

34 Rc identified 98% of previously defined cell essential genes.

35 networks, but predicted substantially fewer essential genes.

36 to study the loss-of-function phenotypes of essential genes.

37 in loss-of-function screens to identify cell essential genes.

38 encoded by subset of the phototrophic growth-essential genes.

39 ion almost absent outside homozygous loss of essential genes.

40 a 3.1-kilobase deletion (mtDNA) lacking four essential genes.

41 physically interact and be co-expressed with essential genes.

42 sh the utility of this strategy for defining essential genes.

43 Cyclin F, which is encoded by an essential gene, also contains a cyclin box domain, but i

44 For each pair in a collection of non-essential genes, an epistasis score is obtained, represe

45 ood because Rap1 is encoded by a single copy essential gene and is involved in many disparate and ess

46 arcode transposon site sequencing (RB-TnSeq) essential gene and physiological data specific to photoa

47 act parasites established that AAH is not an essential gene and that Deltaaah cells are capable of sa

48 We found that many SS/L interactions involve essential genes and are therefore detectable within a li

49 reconstructed models of E. coli, calculating essential genes and comparing with experimental flux dat

50 tive selection, particularly those affecting essential genes and conserved genes.

51 s (TSS), CRISRPi identified 92% of core cell essential genes and did not show a bias to regions invol

52 genesis, both driving the response away from essential genes and directing the pathway toward mRNA ta

53 ol to identify the function of genes, reveal essential genes and generally to unravel the genetic bas

54 They were also more likely to influence essential genes and genes involved in complex disease.

55 de novo gene evolution, particularly for new essential genes and new cancer-associated genes.

56 Studies in these organisms have discovered essential genes and pathways for axon regrowth.

57 ug development through the identification of essential genes and pathways, we have measured competiti

58 ative experimental genetic strategy to study essential genes and reveals effects of regulatory imbala

59 omprehensively predicting nutrient rescue of essential genes and synthetic lethal interactions, and w

60 and will enable exploration of functions of essential genes and undiscovered genes/sites/alleles not

61 rated that this system improves the study of essential genes and was sufficiently robust to eradicate

62 strategy of using the nuclease to target an essential gene, and then linking the effector to a seque

63 egulators that tightly control expression of essential genes, and an engineered addiction module base

64 the growth behavior, nutrient requirements, essential genes, and candidate drug targets in these str

65 cterial species that have well-characterized essential genes, and tested the transferability between

66 bjective function, and the reference list of essential genes; and that predictive ability for single-

67 integrative approach that reliably transfers essential gene annotations between distantly related bac

68 The codons of essential genes are changed to create a DNA sequence as

69 stical properties of biological functions of essential genes are enhanced when we focus on essential

70 Furthermore, essential genes are enriched in protein complexes in bot

71 transposon-insertion mutants for 46% of the essential genes are included in the Drosophila Gene Disr

72 The results show the majority of conserved essential genes are involved in critical cell cycle proc

73 However, features associated with plant essential genes are largely unknown and the full set of

74 nosa strains PAO1 and PA14 demonstrates that essential genes are primarily restricted to the core gen

75 Critical ancestral functions of many essential genes are thus retained in a pathway-specific

76 ation, two of which are encoded by candidate essential genes, are glycosylated.

77 le proteins are more likely to be encoded by essential genes, are more central in protein-protein int

78 LdTyrRS appears to be an essential gene as the chromosomal null mutants did not s

79 tions for essential gene pairs, highlighting essential genes as densely connected hubs.

80 matic characterization and identification of essential genes, as well as the investigation of the int

81 highlights a high degree of conservation of essential genes at the species and genus level, while co

82 ional Cre-mediated excision of the autophagy-essential gene Atg3.

83 cells genetically deficient in an autophagy-essential gene (ATG5).

84 CRISPR-based method to inhibit expression of essential genes based on the inducible expression of an

85 Comparisons of differentially essential genes between Ras-dependent and -independent l

86 gnificantly extending our ability to predict essential genes beyond orthologs.

87 Furthermore, not only is eIF3m an essential gene, but its expression level is also importa

88 identify drug targets, (3) detects not only essential genes, but also essential protein domains, (4)

89 ly the characterization of essential and non-essential genes, but also genes whose disruption leads t

90 al of genomic sequences of essential and non-essential genes by placing loxP sites into a short synth

91 d to define most or all of that chromosome's essential genes by temperature-sensitive lethal mutation

92 Our findings show that essential genes can be distinguished from genes with non

93 te that polymorphic genetic variants in cell essential genes can genetically affect closely linked tu

94 Defective variants that lack essential genes can nevertheless reproduce by coinfectin

95 all seven suppressors analyzed mapped to the essential gene cdsA, which is responsible for the conver

96 A screen of 82% of fission yeast non-essential genes, comprising approximately 3,000 gene del

97 genes under diverse conditions and a revised essential-gene concept that includes all essential genom

98 our results reveal that the transcription of essential genes controlled by T-box riboswitches can be

99 species and genus level, while comparison to essential gene datasets from other gut bacteria identifi

100 ting of a well-conserved coding region of an essential gene, DBR1, and measure relative effects on gr

101 calization pattern of yeast Pex11 in all non-essential gene deletion mutants, as well as in temperatu

102 A ligation sites at close proximity in a non-essential gene demonstrated in E. coli suggests the poss

103 reasing the number of bacterial and archaeal essential genes determined by genome-wide gene essential

104 contributes to progressive Pol II pausing on essential genes during development and to rapid Pol II p

105 tein) was coupled to the transcription of an essential gene (e.g. antibiotic resistance gene) through

106 When the transcription of a target non-essential gene (e.g. green fluorescent protein) was coup

107 Second, when targeting essential genes, efficient mutagenesis of founders is of

108 pre- and postnatal development in the mouse [essential genes (EGs)] are enriched for disease genes an

109 These essential genes encode functions involved in the so-call

110 the rapid evolution of centrosomin (cnn), an essential gene encoding centrosomal protein isoforms req

111 us subtilis strain was isolated in which the essential gene encoding tRNA(Ile)-lysidine synthetase wa

112 Dosage suppression of essential genes encoding RNA polymerase subunits and chr

113 ch, we defined three sets of chromosomal non-essential genes essential for growth during exposure to

114 -promoters Hughes Collection with individual essential genes expressed from a doxycycline-regulated p

115 DNA minicircles (small DNA vectors encoding essential gene expression components but devoid of a bac

116 ta demonstrate that, rather than controlling essential gene expression programs, Drosophila JmjC prot

117 e evolutionary diversity and history of this essential gene family, we searched for maturase homologs

118 ously identified Chlamydomonas fertilization-essential genes fell into related clusters based on thei

119 ila, we identified an E3 ligase, Ubr3, as an essential gene for auditory organ development.

120 kinase 2-associated protein 1 (CDK2AP1), an essential gene for early embryonic development, plays a

121 onal-expression mutant identified gacA as an essential gene for GAS.

122 ogether, these results identify SEMA3E as an essential gene for GnRH neuron development, uncover a ne

123 We identify METTL3 as an essential gene for growth of acute myeloid leukaemia cel

124 By conditionally deleting Dicer, an essential gene for microRNA synthesis, and Pten, a key n

125 other centriole, and cilia number, and as an essential gene for normal Hedgehog signaling during neoc

126 These results suggest that abcc6a is an essential gene for normal zebrafish development and prov

127 i data for TbPMM also showed that this is an essential gene for parasite growth.

128 n in RNA-dependent RNA polymerase 2, another essential gene for the biogenesis of endogenous 24-nt si

129 We further show that hsiE1 is a non-essential gene for the T6SS and suggest that HsiE1 may m

130 brary, we identified CLDN1, OCLN and CD81 as essential genes for both the cell-free entry and the cel

131 form system, we show here that we can delete essential genes for cell wall synthesis and propagate ce

132 particles (DIPs) are virus mutants that lack essential genes for growth.

133 s miRNA was capable of modulating one of the essential genes for induced pluripotent stem cell, KLF4.

134 , we selected a panel of 42 highly expressed essential genes for modification.

135 s cerevisiae was modified by the addition of essential genes for pentose metabolism.

136 tMPK6 and AtMPK4 orthologs, respectively) as essential genes for rice development by finding the pres

137 NAs produced in the absence of piRNAs target essential genes for silencing.

138 Our results link the loss of essential genes for the effector function of CD8(+) T ce

139 we uncovered a role for piRNAs in protecting essential genes from RNA silencing.

140 t can serve as both a research tool to study essential gene function and an applied system for antibi

141 erium tuberculosis pathogenesis, analysis of essential gene function in this slow-growing pathogen re

142 rovides an efficient approach to investigate essential gene function that may be particularly useful

143 l and recombinational "safeguard" control of essential gene function.

144 a framework for systematic investigation of essential gene functions in vivo broadly applicable to d

145 Importantly, mild knockdown of essential gene functions significantly reduced stationar

146 Essential gene functions underpin the core reactions req

147 nts in colorectal carcinoma that can lead to essential gene fusions and other oncogenic events.

148 phate-GlcNAc (UDP-GlcNAc) by deletion of the essential gene GNA1.

149 n and potency of TSGs (STOP genes), OGs, and essential genes (GO genes) on chromosomes can predict th

150 herichia coli cells in which a conditionally essential gene has been deleted.

151 DEG 10, a new release of the Database of Essential Genes, has been developed to accommodate these

152 Most screens for essential genes have assayed a single condition--growth

153 Characteristics shared among essential genes have been uncovered in fungal and metazo

154 atics approach to improve the reliability of essential gene identification is desirable.

155 Our results suggest that other essential genes identified by genetic screens may turn o

156 It is enriched in cell-essential genes identified in a CRISPR screen, as well a

157 A negative selection screen for essential genes identified numerous gene sets correspond

158 gan systems demonstrating that ATP6AP2 is an essential gene implicated in basic cellular mechanisms a

159 are well tolerated, even though some affect essential genes implicated in Mendelian disease.

160 de the first direct evidence that rrp2 is an essential gene in B. burgdorferi.

161 interference, we created knockdowns of every essential gene in Bacillus subtilis and probed their phe

162 HBR1 (hemoglobin response gene 1) is an essential gene in Candida albicans that positively regul

163 Moreover, nesd is an essential gene in Drosophila melanogaster, in which it i

164 tide release factor 3 (eRF3) is a conserved, essential gene in eukaryotes implicated in translation t

165 We show that mzt1/tam4 is an essential gene in fission yeast, encoding a 64-amino aci

166 -binding factor CHD1 as a putative synthetic-essential gene in PTEN-deficient cancers.

167 cancer cell lines, we identify SMARCA2 as an essential gene in SMARCA4 mutant cancer cell lines.

168 n to determine the contribution of every non-essential gene in the genome to the fitness of an organi

169 cagY is an essential gene in the T4SS and has an unusual DNA repeat

170 a component of glycolytic metabolism, as an essential gene in triple-negative breast cancer.

171 This study also illustrates that Snf7 is an essential gene in WCR and its functions are consistent w

172 ether these data demonstrate that PPA2 is an essential gene in yeast and that biallelic mutations in

173 High-dimensional phenotypic analysis of 204 essential genes in 1,368 embryos revealed that cell line

174 ling inducible and reversible suppression of essential genes in a mammalian system.

175 Essential genes in A. thaliana are often single copy or

176 We studied whether PPMOs targeted to essential genes in Acinetobacter lwoffii and Acinetobact

177 tion of double-strand RNAs (dsRNA) targeting essential genes in an insect could cause mortality.

178 Here, we show that essential genes in Arabidopsis thaliana have distinct fe

179 for investigating the biological function of essential genes in archaea.

180 cell-specific deletion of Atg7 or Atg5, two essential genes in autophagy, leads to loss of Treg cell

181 ion libraries, which can be used to identify essential genes in bacteria.

182 generating random, unselectable mutations in essential genes in complex operons have generally preclu

183 wn the search and provides information about essential genes in different cancer types.

184 teome definitions, accounts for 65% of known essential genes in E. coli, and has 78% gene function ov

185 ing the 'knockout-first' allele and identify essential genes in ES cells, including the histone methy

186 When all of the seven-base cleavage sites in essential genes in Escherichia coli were eliminated, the

187 itate investigations into the involvement of essential genes in homologous recombination.

188 variety of approaches capable of identifying essential genes in individual datasets as well as compar

189 A CRISPRi screen for essential genes in K562 cells demonstrates that the larg

190 d diCre as a valuable new tool for analyzing essential genes in Leishmania.

191 iction revealed four genomic islands without essential genes in lengths from 8 to 102 kbp, totaling 7

192 pecific enhancers regulating developmentally essential genes in many species such as worm, fly and hu

193 ns to investigate the temporal resolution of essential genes in mES cells, and to identify novel gene

194 s of the sequences showed, as expected, that essential genes in MTBC were more evolutionarily conserv

195 r classifier to learn traits associated with essential genes in one organism, and applied it to make

196 A tag should facilitate study of the role of essential genes in parasite biology.

197 tematic loss-of-function studies to identify essential genes in particular cancer cell types.

198 ng double-stranded RNAs (dsRNAs) that target essential genes in pest insects.

199 in a systems biology workflow by predicting essential genes in six Streptococcus species and mapping

200 uberculosis that functions as a repressor of essential genes in the cell wall mycolic acid biosynthet

201 ine model of candidiasis in which repressing essential genes in the host was achieved.

202 (shRNA) and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemi

203 As expected, silencing known essential genes in the immunotoxin pathway, such as meso

204 Functional analysis of essential genes in the malarial parasite, Plasmodium, is

205 ficient method for identifying conditionally essential genes in Tn-Seq experiments with high detectio

206 Rapid and accurate identification of new essential genes in under-studied microorganisms will sig

207 immediate utility for investigating roles of essential genes in viral replication and will better ena

208 We systematically replaced essential genes in yeast by their 1:1 orthologs from Esc

209 ual codon substitutions for 10-aa regions of essential genes in yeast.

210 f-function screen and identified a number of essential genes, including the bromodomain and extraterm

211 Rpa1, an essential gene involved in DNA replication and genome ma

212 ermore, CU1276 represses endogenous RPA1, an essential gene involved in many aspects of DNA dynamics,

213 One of the essential genes involved in the autophagic initiation is

214 At the population level, identification of essential genes is accomplished by observing intolerance

215 -associated LDI in splicing regulation of an essential gene linked to a genetic disease.

216 sit through the secretory pathway, NS1 is an essential gene linked to early viral RNA replication.

217 g, we introduced in-frame TAG codons into 22 essential genes, linking their expression to the incorpo

218 stance through a dysfunctional variant of an essential gene may be applicable to other plant-pathogen

219 shape in Caulobacter crescentus requires the essential gene mreB, which encodes a member of the actin

220 Here we report on a screen of >1000 essential gene mutants in Saccharomyces cerevisiae for d

221 mutants, as well as in temperature-sensitive essential gene mutants.

222 es the principle that the ts phenotype of an essential gene mutation can be closely linked to the cat

223 ntative iRAP within the coding region of the essential gene, nadD, greatly reduces its transcriptiona

224 Our high-confidence essential gene network, established using chemical genom

225 , we demonstrated that inactivation of a non-essential gene not previously found linked to colistin r

226 PPMOs targeted to essential genes of A. lwoffii and A. baumannii were bact

227 Using this system to target several essential genes of M. tuberculosis, we achieved marked i

228 ry genes are essential in C. crescentus, the essential genes of two Alphaproteobacteria, Agrobacteriu

229 erated in a chemical mutagenesis screen, for essential genes on the Drosophila X chromosome and descr

230 creen through a collection of 126 mutants in essential genes on the X chromosome to identify candidat

231 essential metabolites, leaked expression of essential genes, or genetic mutations.

232 ensive network maps genetic interactions for essential gene pairs, highlighting essential genes as de

233 genetic distance, much of a given organism's essential gene pool is specific to that organism.

234 We show improved performance of essential gene prediction in the bacterium Yersinia pest

235 thesis, and testing, with retention of quasi-essential genes, produced JCVI-syn3.0 (531 kilobase pair

236 bryo-lethality, indicating that FtsHi1 is an essential gene product.

237 have been designed to control cell growth by essential gene regulation, inducible toxin switches and

238 Enhancers are essential gene regulatory elements whose alteration can

239 f a chemical mark on DNA, is a conserved and essential gene regulatory mechanism.

240 ipotency and self-renewal via controlling an essential gene regulatory module.

241 However, conditional inactivation of essential genes remains a challenge.

242 enes are largely unknown and the full set of essential genes remains to be discovered in any plant sp

243 Essential genes represent critical cellular components w

244 ila neto (neuropilin and tolloid-like) as an essential gene required for clustering of iGluRs at the

245 rd genetic screen, we identified MORC2 as an essential gene required for epigenetic silencing by the

246 th a conditional lethal mutation in lpxC, an essential gene required for lipid A biosynthesis, was re

247 Deletion of essential genes required for autophagy, including Beclin

248 smallest genome among bacteria and lack many essential genes required for biosynthetic and metabolic

249 e critical regulators of the Ras pathway and essential genes required for cell division.

250 esis, controlling the expression of the many essential genes required for making cilia.

251 erated an experimental system in which a non-essential gene, required for pigment production and the

252 arasite life cycle, down-regulation of these essential genes resulted in inhibition of parasite devel

253 creates stronger selection against noise in essential genes, resulting in a decrease in their noise.

254 d to significant advancements in research on essential genes, resulting in a dramatic increase in the

255 Repression of an essential gene results in reduction of transcription lev

256 We observed that 91% of tested essential genes retained functionality with limited fitn

257 The profile of essential genes revealed that, compared with Escherichia

258 Using an E. coli essential gene screen, we demonstrate that Cas13b has a

259 ds, antibiotic gene cluster predictions and 'essential gene screening' to provide an interactive page

260 The validity of the essential gene set is supported by its tight overlap wit

261 ng the organism's physiology and defines the essential gene set required for the growth of a photosyn

262 %.These results proved that dsRNAs targeting essential genes show great potential to control C. brunn

263 ludes a maximum-likelihood algorithm to call essential genes simultaneously under multiple conditions

264 of fitness to the expression fluctuations of essential genes than nonessential genes creates stronger

265 datasets from other gut bacteria identified essential genes that appear specific to bifidobacteria.

266 utagenesis methods revealed a class of quasi-essential genes that are needed for robust growth, expla

267 The existence of essential genes that are not inherited from the most rec

268 can be reversed by targeting chromosomal non-essential genes that are not responsible for acquired re

269 we show that this Bayesian approach predicts essential genes that correspond well with genes shown to

270 is restricted by the expression of multiple essential genes that depend on exogenously supplied synt

271 rs of cholinesterase (Ric-8)A and Ric-8B are essential genes that encode positive regulators of heter

272 0B Rho GTPase activating proteins (GAPs) are essential genes that have distinct, but overlapping role

273 ection tends to eliminate those mutations in essential genes that lead to greater changes of MFE valu

274 None of the essential genes that we identified are in a 200-kb regio

275 dent test set of more than 300 essential/non-essential genes that were not used to train the model, t

276 Here, we report that ptc-3 is also an essential gene; the absence of ptc-3 results in a late e

277 We sought to identify 'synthetic-essential' genes: those that are occasionally deleted in

278 ow that human disease genes are enriched for essential genes, thus providing a dataset that facilitat

279 We targeted an essential gene to develop disabled virus strains as vacc

280 ly 25% of the in vivo S. aureus mono-culture essential genes to non-essential.

281 in six Streptococcus species and mapping the essential genes to small molecule ligands from DrugBank.

282 Growth-based screens identify essential genes, tumor suppressors, and regulators of di

283 ease in the number of identified prokaryotic essential genes under diverse conditions and a revised e

284 components, we systematically evaluated 870 essential genes using a library of repressible-promoter

285 's predictions with a genome-wide screen for essential genes using a transposon-directed insertional

286 precision of the two libraries in detecting essential genes was similar and that combining data from

287 To investigate the origin and evolution of essential genes, we identified and phenotyped 195 young

288 ntial domains to reproduce the annotation of essential genes, we received accurate results that sugge

289 Our screen revealed the set of cell-essential genes, which was validated with an orthogonal

290 Both AtTAD2 and AtTAD3 are encoded by essential genes whose knockout is lethal and leads to ar

291 Identification of essential genes will allow focused research on minimal S

292 vidual models to become active, and predicts essential genes with a higher positive predictive value.

293 ll analysis shows that we detect over 90% of essential genes with minimal false positives using a com

294 This study evaluates the role of essential genes with respect to pathogen virulence in a

295 s unknown, we isolated and characterized the essential genes within the 640-kb region of the third ch

296 We identify specific DNA amplifications and essential genes within these amplicons representing key

297 l genes as well as an additional 1970 likely essential genes without documented phenotypes.

298 We also posited that such synthetic-essential genes would be therapeutic targets in cancers

299 Notably, dysregulation of these essential genes would significantly impair synaptic func

300 n is encoded by the hitherto uncharacterized essential gene, YNL260c.