ライフサイエンスコーパス: ethidium bromide

1 after cell staining with acridine orange and ethidium bromide).

2 gand, and to the classic intercalating agent ethidium bromide.

3 and measured mtDNA after 3-d treatment with ethidium bromide.

4 ethidine in the extracellular environment to ethidium bromide.

5 mycin, bisphenol A, chlorinated phenols, and ethidium bromide.

6 ed by gel electrophoresis in the presence of ethidium bromide.

7 et by fluorescence after brief staining with ethidium bromide.

8 demyelinated by the intraspinal injection of ethidium bromide.

9 by agarose concentration or the presence of ethidium bromide.

10 by x-ray irradiation and focal injections of ethidium bromide.

11 d after electrophoresis in 1.5% agarose with ethidium bromide.

12 oethidium, a membrane-permeant derivative of ethidium bromide.

13 x-irradiation and intraspinal injections of ethidium bromide.

14 ly studied MDR substrates, Hoechst 33342 and ethidium bromide.

15 sible DRB4 band in agarose gels stained with ethidium bromide.

16 equires UV radiation and the fluorescent dye ethidium bromide.

17 induced upon incubation with erythromycin or ethidium bromide.

18 er rapidly, as revealed after treatment with ethidium bromide.

19 ntibiotics based on the fluorescent molecule ethidium bromide.

20 served fluorescently after labeling DNA with ethidium bromide.

21 thus eliminating the need for staining with ethidium bromide.

22 ophoresis on a 0.8% agarose gel stained with ethidium bromide.

23 agarose gel electrophoresis and stained with ethidium bromide.

24 d in increased accumulation of intracellular ethidium bromide.

25 t)DNA by passaging in a low concentration of ethidium bromide.

26 on uridine after eight passages in 50 ng/mL ethidium bromide.

27 nd intercalating ligands: DAPI, Hoechst, and ethidium bromide.

28 Of the 6 compounds that did not displace ethidium bromide, 2 also inhibited B-ZIP binding to DNA

29 The cation of the salt ethidium bromide (3,8-diamino-5-ethyl-6-phenylphenanthri

30 ansfected with hCx31.9-EGFP took up DAPI and ethidium bromide 5-10 times faster than wild-type cardio

31 More importantly, cytosine arabinoside, ethidium bromide, 5-azacytidine and aspirin all signific

32 dino-2-phenylindone (an AT-specific binder), ethidium bromide (a nonspecific binder), and chromomycin

33 oparticles capped with DNA intercalated with ethidium bromide, a fluorescent molecule.

34 optosis was determined by DNA fragmentation, ethidium bromide-acridine orange nuclear stain and TdT-m

35 PI viability was examined with ethidium bromide-acridine orange, and apoptosis was exam

36 DNA fragmentation and ethidium bromide/acridine orange (EB/AO) nuclear stainin

37 ted for apoptosis either by staining with an ethidium bromide/acridine orange mixture (AO/EB) or with

38 viable cell recovery (VCR) with trypan blue, ethidium bromide/acridine orange staining, and terminal

39 aternary ammonium on an aromatic ring (e.g., ethidium bromide, acriflavine hydrochloride, 2-N-methyle

40 PMNL were stained with acridine orange and ethidium bromide after 0, 3, 6, and 18 h in culture, and

41 ed products were visualized by staining with ethidium bromide after electrophoresis in 1.5% agarose.

42 ; staining at the single-molecule level with ethidium bromide after exhaustive deproteinization of ly

43 Ethidium bromide also inhibited NaeI-L43K, implying that

44 Unwinding the supercoiled DNA with ethidium bromide also made DNA resistant to AN/L3.

45 Ethidium bromide and 2',7'-dichlorofluorescein, fluoresc

46 The intercalating agents ethidium bromide and 9-aminoacridine enhanced oxopropeny

47 The system uses dye-based detection with ethidium bromide and a single DNA polymerase-based PCR o

48 nalyzed from single islet cells stained with ethidium bromide and acridine orange, apoptosis using a

49 ld decrease in sensitivity to quinolones and ethidium bromide and an increase in the level of norA tr

50 MDR-dependent efflux of ethidium bromide and berberine from S. aureus cells was

51 ycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumu

52 For comparison, the unwinding angles of ethidium bromide and DA have been independently calculat

53 NA intercalating agents propidium iodide and ethidium bromide and enhanced by the presence of synthet

54 ical and B-DNA, displacement of intercalated ethidium bromide and facilitate cooperative binding of H

55 the displacement of DNA duplex intercalated ethidium bromide and gel electrophoresis.

56 binds to genomic DNA to a similar extent as ethidium bromide and Hoechst 33258.

57 Fluorescent quenching of ethidium bromide and of rhodamine covalently attached to

58 thod is at least 50-fold more sensitive than ethidium bromide and permits detection of </=0.25 ng dou

59 Decreased accumulation of ethidium bromide and rhodamine 6G in the hns mutant comp

60 -ethylmaleimide but is strongly inhibited by ethidium bromide and vanadyl ribonucleoside complexes.

61 ramphenicol), transcription and replication (ethidium bromide), and function (rotenone, rhodamine 6G)

62 ase in resistance to hydrophilic quinolones, ethidium bromide, and cetrimide and also to sparfloxacin

63 rmeable to Lucifer yellow, Alexa Fluor(350), ethidium bromide, and DAPI, which have valences of -2, -

64 cobacterium smegmatis is more susceptible to ethidium bromide, and drug resistance is restored by the

65 nts are more sensitive than the wild type to ethidium bromide, and K. lactis sir4 mutants are more re

66 rculosis iniA in BCG conferred resistance to ethidium bromide, and the deletion of iniA in M. tubercu

67 resensitization of Hsmr-expressing cells to ethidium bromide; and was non-hemolytic to human red blo

68 o oligonucleotides with model intercalators (ethidium bromide andactinomycin D) and minor groove bind

69 imal sample preparation is possible by using ethidium bromide as the intercalating dye.

70 the use of simple DNA intercalators, such as ethidium bromide, as tools to facilitate the error-free

71 lectrophoretic mobilities in the presence of ethidium bromide before and after relaxation by calf thy

72 th DNA in electrophoretic mobility shift and ethidium bromide binding assays.

73 interstrand cross-links were measured by the ethidium bromide binding fluorescence assay and quantita

74 More than one ethidium bromide binding site is found in the acceptor s

75 Metal ions also weaken ethidium bromide binding to IRE-RNA with no effect on IR

76 ns in the DNA helix as detected by decreased ethidium bromide binding.

77 Low (micromolar) concentrations of ethidium bromide block RNase III[DeltadsRBD] cleavage of

78 Unlike ethidium bromide, both eilatin and the eilatin-containin

79 from sodium dodecyl sulfate, novobiocin, and ethidium bromide but failed with other known substrates

80 der-surpassing the sensitivity achieved with ethidium bromide by 200-fold.

81 binding (measured as accessibility of DNA to ethidium bromide by electrophoresis and by fluorescence

82 Moreover, ethidium bromide can be readily removed using isoamyl al

83 We show that ethidium bromide can influence DNA self-assembly, decrea

84 hat DXR and other DNA intercalators, such as ethidium bromide, can rapidly intercalate into mtDNA wit

85 In addition to hypersensitivity to ethidium bromide, cells that lack the lprG-Rv1410c opero

86 e insertion efficiency, and to resistance to ethidium bromide collectively demonstrate that EmrE mono

87 o footprinting of the latter showed ATP- and ethidium bromide-dependent modifications that could be c

88 Ethidium bromide depleted both mutant and normal cells o

89 nding affinity of polyamines to DNA using an ethidium bromide displacement assay showed that homologu

90 o screened for DNA binding efficacy using an ethidium bromide displacement assay.

91 e, circular dichroism, linear dichroism, and ethidium bromide displacement assays, which demonstrated

92 formation (by 2-aminopurine fluorescence and ethidium bromide displacement); (ii) metal ions increase

93 contrast, other DNA-binding agents, such as ethidium bromide, distamycin, and doxorubicin, inhibit t

94 ransfer takes place between DNA-intercalated ethidium bromide (DNA-EB) and the electrostatically boun

95 Ethidium bromide does not promote the formation of any o

96 showed potent synergistic activity with the ethidium bromide dye in a strain overexpressing the MepA

97 ble to remyelinate demyelinated axons inside ethidium bromide (EB) demyelination lesion in adult spin

98 ircular dichroism (CD) spectroscopy, and the ethidium bromide (EB) displacement assay.

99 rbance we measured changes in geometric mean ethidium bromide (EB) fluorescence intensities in subpop

100 rescence resonance energy transfer (FRET) to ethidium bromide (EB) intercalated within double-strande

101 Ethidium bromide (EB) is known to inhibit cleavage of ba

102 ng; energy is transferred from the CCP to an ethidium bromide (EB) molecule intercalated into the dsD

103 a cells depleted of mtDNA via treatment with ethidium bromide (EB) were found to contain reduced stea

104 abeled with fluorescein amidite (FAM-ssDNA), ethidium bromide (EB), and graphene oxide (GO) are emplo

105 complexation with the phenanthridinium drug ethidium bromide (EB).

106 solutions by monitoring the amount of bound Ethidium Bromide (EB).

107 r previously by the intraspinal injection of ethidium bromide (EB).

108 TM4(85-105) sequence inhibits Hsmr-mediated ethidium bromide efflux from bacterial cells.

109 Because ethidium bromide efflux is an energy-dependent process a

110 The procedure developed here using bacterial ethidium bromide efflux pump activity may be a useful co

111 tions of the intercalative drugs, except for ethidium bromide, enhance production of topoisomerase--D

112 ed field gel electrophoresis (PFGE) and CsCl/ethidium bromide equilibrium centrifugation demonstrates

113 We utilize ethidium bromide (EtBr) as a model intercalator to demon

114 ine) lipids was quantified by an increase of ethidium bromide (EtBr) fluorescence.

115 tween genomic DNA and the intercalating drug ethidium bromide (EtBr) have been determined by use of a

116 Zn(2+) complexation inhibited ethidium bromide (EtBr) intercalation and stabilized FdU

117 al effects of binding the intercalating drug ethidium bromide (EtBr) to 160 base pair (bp) fragments

118 vo mtDNA polymerase activity assay utilizing ethidium bromide (EtBr) to deplete mtDNA, showed that po

119 hemichannel activity as evident by enhanced ethidium bromide (EtBr) uptake that could be blocked by

120 ility and a decreased ability to intercalate ethidium bromide (EtBr).

121 Currently, ethidium bromide (EthBr) is the cheapest and most used D

122 potential of several dyes [acridine orange, ethidium bromide, ethidium homodimer, bis-benzimide (DAP

123 as determined by mitochondrial function and ethidium bromide exclusion, was not inhibited by the bro

124 Gel electrophoresis and ethidium bromide experiments showed that 9a-9c associate

125 /mL culture produced significantly increased ethidium bromide fluorescence compared to nonexposed con

126 Ethidium bromide fluorescence intensities increased upon

127 roxide anion production was measured with an ethidium bromide fluorescence method.

128 tocol to quantify PCR products, by measuring ethidium bromide fluorescence of PCR products excised fr

129 Ethidium bromide fluorescence of the product DNA was use

130 measured by lucigenin chemiluminescence and ethidium bromide fluorescence) and impaired endothelium-

131 (measured by lucigenin chemiluminescence and ethidium bromide fluorescence) that was inhibited or red

132 s of A vessels produced O(2)(.-) (shown with ethidium bromide fluorescence).

133 Data from circular dichroism, inhibition of ethidium bromide fluorescence, interstrand cross-linking

134 DNA cross-linking, assayed by ethidium bromide fluorescence, was significantly inhibit

135 en the amplified products were visualized by ethidium bromide fluorescence.

136 polyamines retain their ability to displace ethidium bromide from calf thymus DNA and are rapidly ta

137 se polyamines retain the ability to displace ethidium bromide from calf thymus DNA and are rapidly ta

138 It displaces 50% of ethidium bromide from DNA at a charge ratio (+/-) of 0.9

139 grouped depending on whether they displaced ethidium bromide from DNA.

140 ed with Southern blot analysis compared with ethidium bromide gel electrophoresis (EtBr) for all mRNA

141 ed with PCR analysis as a 173-bp fragment on ethidium bromide gels.

142 and the bound plasmids were eluted using an ethidium bromide gradient.

143 These observations suggest that ethidium bromide has a preferred intercalation site clos

144 Ethidium bromide has served as a classic DNA intercalato

145 drugs including echinomycin, actinomycin-D, ethidium bromide, Hoechst 33342, and cis-C1 were subject

146 Uptake of ethidium bromide (i) was faster in Cx43 and Cx43-EGFP th

147 rsed both tolerance to INH and resistance to ethidium bromide in BCG.

148 p inhibitor reserpine inhibits resistance to ethidium bromide in both wild-type M. smegmatis and the

149 Inclusion of ethidium bromide in the reaction mixture leads to a grea

150 An insertion mutant of the ethidium bromide-induced all7631 did not show any signif

151 lt rat sciatic nerves into X-irradiation and ethidium bromide-induced demyelinated dorsal column lesi

152 e investigated the effect of previous focal, ethidium bromide-induced demyelination of brain stem whi

153 Following ethidium bromide injection there was a decrease in secur

154 stranded DNA with hybridization detected via ethidium bromide intercalation, further establishing tec

155 Titration of ethidium bromide into the assay decreased activity to a

156 We used injections of the gliotoxin ethidium bromide into the dorsal funiculus of the cervic

157 is responsible for drug resistance and that ethidium bromide is a novel substrate for P55.

158 x and 543-nm excitation for the detection of ethidium bromide-labeled nucleic acids (i.e., RNA).

159 ed fluorescence (LIF) was employed to detect ethidium bromide-labeled RNA molecules under native cond

160 lls were transplanted into the X-irradiation/ethidium bromide lesioned dorsal columns of immunosuppre

161 embrane permeable DNA-associating vital dye, ethidium bromide monoacetate (visible wavelength single

162 Ethidium bromide no longer intercalates between base pai

163 cted by staining with either acridine orange/ethidium bromide or annexin-V-fluorescein/propidium iodi

164 ignals that could be reversed by addition of ethidium bromide or by DNA melting, suggesting that flav

165 of the protein association to treatment with ethidium bromide or micrococcal nuclease.

166 ed protein response in wild-type worms using ethidium bromide or paraquat triggered statin resistance

167 Fluorescence-based binding assays that use ethidium bromide or Rev peptide displacement are used to

168 Twisting was controlled using ethidium bromide or SYBR Green I as model intercalators.

169 luorescence derived from the displacement of ethidium bromide or thiazole orange from the DNA of inte

170 Animals received ethidium bromide plus photon irradiation producing discr

171 rophoresis in agarose gels and staining with ethidium bromide, produced DNA fragments in the 4.0- to

172 Films treated with ethidium bromide prompt switching of dsDNA to ssDNA befo

173 Further, acridine orange, ethidium bromide, propidium iodide and DAPI staining dem

174 g a DNA-targeting intercalating agent (i.e., ethidium bromide) resulted in a marked shift of the clea

175 tion between caffeine and acridine orange or ethidium bromide results in singlet-state lifetime incre

176 erine and palmatine and the DNA intercalator ethidium bromide, revealed a change in the absorbance an

177 Ethidium bromide selectively intercalates into the accep

178 caffeine and the nonplanar DNA intercalator ethidium bromide show optical shifts and steady-state fl

179 nes were confirmed as Salmonella specific on ethidium bromide-stained agarose gels by Southern hybrid

180 electrophoresis and uv transillumination of ethidium bromide-stained agarose gels we and others have

181 by BrdU uptake and cell counts of calcein AM/ethidium bromide-stained cells.

182 by visualizing 1.1- to 1.2- kb PAN RNA in an ethidium bromide-stained gel from poly(A)-selected RNA.

183 The ethidium bromide-stained gels are photographed or scanne

184 ) stain, confocal fluorescence microscopy of ethidium bromide-stained sections, electron microscopy,

185 transcription-PCR amplification followed by ethidium bromide staining (PCR-ETBr) or nucleic acid hyb

186 on of microsatellite changes: (a) silver and ethidium bromide staining of polyacrylamide gels; (b) ra

187 The results of gel electrophoresis and ethidium bromide staining of the DNA fingerprints obtain

188 n to orange/yellow shifts on acridine orange/ethidium bromide staining, and cell surface annexin V bi

189 s of total DNA in an agarose gel followed by ethidium bromide staining, and subsequent scanning of th

190 as 100 viable trophozoites as determined by ethidium bromide staining, while no signal was obtained

191 sualized after separation in agarose gels by ethidium bromide staining.

192 ll apoptosis as evidenced by acridine orange-ethidium bromide staining.

193 sis on 2 % agarose gels, and visualized with ethidium bromide staining.

194 aphy, SYBR Gold stain is more sensitive than ethidium bromide, SYBR Green I stain, and SYBR Green II

195 Binding of ethidium bromide to Escherichia coli tRNAVal and an RNA

196 ma COLO 16 cells were chronically exposed to ethidium bromide to inhibit mitochondrial DNA synthesis

197 Further studies using acridine orange and ethidium bromide to measure apoptosis revealed that mdr1

198 Binding ethidium bromide to one of these RNA fragments, which wo

199 Strikingly, both ethidium bromide transport and normal cell surface prope

200 se chain reaction of isolated total RNA from ethidium-bromide-treated and untreated cells.

201 educed mitochondrial DNA (mtDNA) contents by ethidium bromide treatment or myocytes treated with know

202 Reduction of mtDNA content in DRHEp2 by ethidium bromide treatment reduced the resistance.

203 everal mtDNA forms after severe depletion by ethidium bromide treatment showed that replication and m

204 that were rendered respiration deficient by ethidium bromide treatment.

205 Ethidium bromide uptake assays revealed increased envelo

206 urrent, and, when expressed in HEK293 cells, ethidium bromide uptake was only approximately 5% that o

207 as indicated by BzATP-mediated Ca2+ influx, ethidium bromide uptake, and lactate dehydrogenase relea

208 nfrequent or brief opening could account for ethidium bromide uptake.

209 lity of pgs1Delta to grow in the presence of ethidium bromide was due to defective cell wall integrit

210 Ethidium bromide was used as the intercalating dye for l

211 proteins during recovery from treatment with ethidium bromide, when mtDNA replication is stimulated i

212 e to treatment of the organelles with ATP or ethidium bromide, which affects differentially the rates

213 Ethidium bromide, which binds reversibly to DNA via inte

214 elination in rodent CNS in the X-irradiation/ethidium bromide (X-EB) model.

215 emyelinating lesions had been produced using ethidium bromide/X-irradiation.