ライフサイエンスコーパス: exo-

1 with high selectivity (typically >98:<2 endo:exo).

2 ers (7-phenyl 51-96% exo and 7-acetyl 54-96% exo).

3 their imidazole-bearing sidechains outward (exo).

4 The tagged gene was named EXORDIUM (EXO).

5 oprecipitation-exonuclease methodology (ChIP-exo).

6 eated chromatin-immunoprecipitated DNA (ChIP-exo).

7 kinetic inducement for replication by Klenow exo-.

8 erformed in vitro utilizing DNA polymerase I exo-.

9 edral isomer energies (E(isom) = E(endo) - E(exo)).

10 ragment of Escherichia coli polymerase I (Kf exo+).

11 bus solfataricus P2 DNA polymerase B1 (PolB1 exo-).

12 as retained (exo+) or removed by sonication (exo-).

13 cretion of type 3 exotoxins (ExoU, ExoT, and ExoS).

14 ducts when replication was conducted with Kf exo(+).

15 studies were conducted with Klenow fragment exo(-).

16 ol(M) Exo(+)), and the double mutator Pol(M) Exo(-).

17 hesis was much more efficient with Pol delta-exo(-).

18 Several isomers of 7-methyl-2-exo-([(18)F]fluoropyridinyl-5'-pyridinyl)-7-azabicyclo[2

19 ocedures were developed for the synthesis of exo-(2'-chloro-5-pyridinyl)-7-(endo and exo)-amino[2.2.1

20 c-5 leading to the endo-[4 + 2] (endo-2) and exo-[2 + 2] (anti-3) cycloadducts.

21 Both exo (21) and endo (27) isomers of the metabolite 2 were

22 2-exo-(3'-Amino-2'-chloro-5'-pyridinyl)-7-azabicyclo[2.2.1

23 f the radiolabeled compounds, (-)-7-methyl-2-exo-[3'-(2-[18F]fluoropyridin-5-yl))-5'-pyridinyl]-7-aza

24 ding affinity (Ki = 0.3 nM) ((-)-7-methyl-2- exo-[3'-(6-[(18)F]fluoropyridin-2-yl)-5'-pyridinyl]-7-az

25 ction of 1-BF3 with Cp 2 yields the expected exo [4 + 2] cycloadduct, the reactions of these FHCs yie

26 The activation enthalpy for the concerted exo-[4 + 2] cycloaddition (exo-2TS, DeltaH(double dagger

27 ExoS (453 amino acids) is a bi-functional type III cytot

28 ExoS (453 amino acids) is a bi-functional type-III cytot

29 ExoS (453 amino acids) is a bifunctional type III cytoto

30 otonation and the formation of endo (4a) and exo (4b) isomers of trans-[HFe(PNHP)(dmpm)(CH(3)CN)](BPh

31 n solvents, "S(N)i" reactions lead mainly to exo- (4g) and endo-2-chloro-5-norbornenes (4g').

32 e doubly convergent route employs a tandem 5-exo, 6-exo radical cyclization as the key step.

33 UDG that is incorporated into DNA by Klenow exo(-), a model replicative polymerase.

34 Recently, MSC-derived exosomes (MSC-Exos), a type of microvesicle released from MSCs, were t

35 The polymerase (pol) and exonuclease (exo) activities are spatially separated in different enz

36 erally have a more potent 3'-5' exonuclease (exo) activity than A family DNA polymerases that mainly

37 ence of an associated 3'- to 5'-exonuclease (exo) activity.

38 ure cleanly and regioselectively via formal "exo" addition of the nitrogen-hydrogen bond to the pende

39 Pseudomonas aeruginosa exoenzyme S (ExoS) ADP-ribosylates multiple eukaryotic targets to pro

40 One of these effectors, ExoS, ADP-ribosylates several host cell proteins, includ

41 lar substrates of TTS-translocated ExoS (TTS-ExoS) ADPRT activity include proteins in the Ras superfa

42 s of exo-(2'-chloro-5-pyridinyl)-7-(endo and exo)-amino[2.2.1]heptanes (3a and 3b).

43 Chromatin immunoprecipitation-exo, an emerging technique using lambda exonuclease to d

44 omatin immunoprecipitation-exonuclease (ChIP-exo) analysis shows that Nap1 is required for H2A-H2B de

45 r(567) in the polymerase active site (Pol(M) Exo(+)), and the double mutator Pol(M) Exo(-).

46 quence: NNCA(G/C)TGNN decreasing), and Vent (exo(-)) and Vent DNA polymerase.

47 mparing the mutational spectra of the Pol(+) Exo(-) and Pol(+) Exo(+) enzymes revealed the patterns a

48 utilized for studying the activity of Klenow exo(-) and repair enzymes on templates containing the le

49 of the C2'-oxidized abasic site with Klenow exo(-) and repair enzymes suggest that the lesion will b

50 (Z-5-exo), E-3-methylenisoindolin-1-one (E-5-exo), and isoquinolinone (6-endo).

51 s derived from HCV-infected hepatocytes (HCV-exo), and the expression of fibrosis-related genes was e

52 erived from two protons [Fe(III)-bound C5-OH(exo) and C5-H(endo)] from camphor.

53 positions to form the isomeric exocyclic (1-exo) and endocyclic (1-endo) dienolates.

54 t, E176A, which is deficient in exonuclease (EXO) and gap endonuclease (GEN) activities but retains a

55 s mutant protein lacks the FEN, exonuclease (EXO) and gap endonuclease (GEN) activities of FEN1 but r

56 ition in H(2)O or D(2)O buffer, both ENDOR H(exo) and H(endo) signals are absent.

57 s of amino acid substitutions caused by POLE-exo* and compare them to other tumor types.

58 iabetic NOD mice for their reactivity to the EXO, and compared this reactivity between diabetes-susce

59 p analytic methods are not tailored for ChIP-exo, and thus cannot take full advantage of high-resolut

60 tes in PCR reactions catalyzed by Taq, Vent (exo-) and Deep Vent (exo-) polymerases, with 94.4%, 97.5

61 Type II archeon DNA polymerases Vent (exo-) and Pfu (exo-) can be utilized for PAP or a bidire

62 rformed with complete control of the regio-, exo-, and enantioselectivity under aminocatalytic condit

63 rgoes a [1,3] sigmatropic rearrangement to 5-exo- and 5-endo-methoxybicyclo[2.2.2]oct-2-enes, 2a and

64 The golden state: selective 5-exo- and 6-endo-cyclizations of an alkynyl benzothioamid

65 P-stereogenic heterocycles featuring both an exo- and an endocyclic double bond.

66 help explain how RNase BN can act as both an exo- and an endoribonuclease and also demonstrate that i

67 i, ionotropic and metabotropic P2 receptors, exo- and ecto-nucleotidases, second messengers, and gap

68 [(-)-cis-1 and (+)-trans-2] and two isomers, exo- and endo-3-isopropyl-6-methylbicyclo[3.1.0]hex-2-en

69 in the ion pairs, product distributions from exo- and endo-7 differed, with more endo-chloride formed

70 fy effectiveness of this array for detecting exo- and endo-acting glycoside hydrolase activity using

71 to CD166 was not disruptable by a variety of exo- and endo-glycosidases, implying recognition of a no

72 tion products, we successfully distinguished exo- and endo-hydrolases and found high taxonomic variat

73 hat iridium and iron moieties have exchanged exo- and endo-polyhedral sites with respect to the 10-ve

74 itution of compounds 3-6 is such that latent exo- and endo-receptor properties are simultaneously pre

75 aptic vesicle endocytosis and may facilitate exo- and endocytic coupling.

76 Synaptic vesicle (SV) exo- and endocytosis are tightly coupled to sustain neur

77 Synaptic vesicle exo- and endocytosis are usually driven by neuronal acti

78 We monitored the spatial distribution of exo- and endocytosis at 37 degrees C in mouse motor nerv

79 We uncoupled the function of syt1 in exo- and endocytosis in mouse neurons either by re-targe

80 Here we review these exo- and endocytosis modes and their roles in regulating

81 urotransmission relies on repeated cycles of exo- and endocytosis of the synaptic vesicles (SVs), in

82 ciated protein involved in the regulation of exo- and endocytosis processes at presynaptic sites.

83 a, we have obtained estimates of adiponectin exo- and endocytosis rates, and we have predicted adipon

84 The effect of these manipulations on exo- and endocytosis were analyzed using electrophysiolo

85 rprisingly, FM1-43 labeling indicates normal exo- and endocytosis, but a specific inability to mobili

86 e-associated processes, including autophagy, exo- and endocytosis, phagocytosis and immune response.

87 ed either by membrane folding or by membrane exo- and endocytosis.

88 n cycle with SVs that parallels the cycle of exo- and endocytosis.

89 he reservoir is set by the relative rates of exo- and endocytosis.

90 lation of many cellular functions, including exo- and endocytosis.

91 priming of the vesicles for another round of exo- and endocytosis.

92 modulated in parallel with synaptic vesicle exo- and endocytosis.

93 4-dependent signaling cassette and perturbed exo- and endocytosis.

94 mical analysis failed to uncover substantial exo- and endocytotic traffic of the channel.

95 unctional and structural relationship of the exo- and endodomain, signal transduction, and receptor g

96 , interacting (allosteric), and co-existent, exo- and endofacial GLUT1 ligand-binding sites.

97 utperforms mixtures of commercially relevant exo- and endoglucanases.

98 d 37 degrees C through absolutely quantified exo- and endometabolomics data.

99 nit and retains significant, albeit reduced, exo- and endonuclease activities but does not undergo ph

100 s of these substrates before and after their exo- and endonucleolytic cleavage, as well as structures

101 re functional diversity, while Gfh1 inhibits exo- and endonucleolytic cleavage, RNA synthesis, and py

102 etabolised by a number of enzymes, including exo- and endopolyphosphatases.

103 titutions, and tested their response to both exo- and endovanilloids.

104 retained hPCNA-mediated stimulation of both exo- and flap endonuclease activities.

105 ctors that funnel RNA substrates to abundant exo- and/or endonucleases.

106 type III secretion proteins (PopD, PcrV, and ExoS) and a flagellum-associated protein (FliD).

107 one of the miRNAs overexpressed in obese ATM Exos, and earlier studies have shown that PPARgamma is a

108 cterial strain that naturally secretes ExoU, ExoS, and ExoT were generated to carefully evaluate the

109 biochemical and enzymatic functions of ExoU, ExoS, and ExoT, three effector proteins secreted by this

110 e type III secretion system effectors (ExoU, ExoS, and PcrV) and for strain relatedness using randoml

111 e pathogenesis of P. aeruginosa keratitis in ExoS- and ExoT-producing strains is almost entirely due

112 omatin immunoprecipitation-exonuclease (ChIP-exo) approach, we find that agonist-liganded human andro

113 Exosomes (EXO) are secreted intracellular microparticles that can

114 Exosomes (EXOs) are secreted, nano-sized membrane vesicles that co

115 C2-AP lesion has a similar effect on Klenow exo(-) as do AP and C4-AP sites.

116 tion 4(R)Hyp in this structure is up (Cgamma exo), as has been found in other collagen peptide struct

117 atin at near-base-pair resolution using ChIP-exo, as RPGs are coordinately reprogrammed.

118 sociated with the ADP-ribosylating domain of ExoS, as bacteria expressing plasmids lacking expression

119 to 99 degrees C (T(end)) and 68 degrees C (T(exo)) ([AsF(6)(-)]:[PF(6)(-)] = 0.4:0.6).

120 hage lambda genome comprising the red genes (exo, bet and gam) under their native control.

121 s are used for the recombination, the lambda Exo, Beta, and Gam proteins are required.

122 situated metal binding site for an exterior (exo) binding site.

123 PolB1 exo- binds tightly to DNA (K(d)(DNA) = 1.8 nM) and a cor

124 ested several DNA polymerases and found that exo(-) Bst DNA polymerase meets the requirement for high

125 Invasive P. aeruginosa secrete ExoT and ExoS, but exsA mutation has little impact on their short

126 e ADP-ribosyltransferase (ADP-r) activity of ExoS, but not the Rho-GAP activity nor the membrane loca

127 ely charged residues establish the correct N(exo)/C(cyt) topology of the peptide, in accordance with

128 ons that are consistent with the Type III (N(exo)/C(cyt)) topology.

129 Nano-sized cellular vesicles like exosomes (Exo) can ferry GNP-therapeutic complexes without causing

130 archeon DNA polymerases Vent (exo-) and Pfu (exo-) can be utilized for PAP or a bidirectional form of

131 e confirm our observations by analyzing ChIP-exo, chemical mapping, and ATAC-seq data from other labo

132 We applied high-resolution ChIP-exo (chromatin immunoprecipitation followed by 5'-to-3'

133 Using ChIP-exo (chromatin immunoprecipitation with lambda exonuclea

134 sylation of a non-G-protein substrate of TTS-ExoS, cyclophilin A (CpA), a peptidyl-prolyl isomerase (

135 k, termed MACE (model-based analysis of ChIP-exo) dedicated to ChIP-exo data analysis.

136 n the palm domain of the parental enzyme, an exo(-) derivative of RB69 DNA polymerase (gp43), a membe

137 dCDP onto a 13/20mer primer/template with an exo(-) derivative of RB69 DNA polymerase and have shown

138 report development of an Exposure Ontology, ExO, designed to address this information gap by facilit

139 Deep Vent (exo(-)) DNA polymerase accepted the nucleotide triphosph

140 ension experiments using Escherichia coli Kf exo(-) DNA polymerase were carried out under "standing s

141 catalyze endonucleolytic and exonucleolytic (EXO) DNA hydrolyses.

142 mplate for primer extension using deep vent (exo-) DNA polymerase, thereby enabling the regeneration

143 h could be synthesized in 1 h by the Klenow (exo-) DNA polymerase.

144 t in substitutions in the 3'-5' exonuclease (Exo) domain of the catalytic subunit of the viral DNA po

145 primer terminus between pol and exonuclease (exo) domains was compromised with the L415A and L415G mu

146 tion of 6 in extended products by Deep Vent (exo(-)) during PCR or by Sequenase during copying of sin

147 products: Z-3-methylenisoindolin-1-one (Z-5-exo), E-3-methylenisoindolin-1-one (E-5-exo), and isoqui

148 and excellent stereoselectivities toward the exo-(E) adducts are obtained on a wide range of substrat

149 concentrations by formation of E(2).Mg.(INT)(exo):E(1)[ATP].Mg.(H(+)) which is converted to E(2).Mg.(

150 g.(H(+)) which is converted to E(2).Mg.(INT)(exo):E(1)P.Mg.(H(+))(cyto), but this E(1)P form was K(+)

151 rases have a separate 3' --> 5' exonuclease (exo) editing activity that is involved in assuring the f

152 ps of translesion DNA synthesis by RB69 gp43 exo-, employing a non-natural purine triphosphate analog

153 When Pol delta-exo(-) encountered a downstream primer, it paused with 3

154 embrane protein turnover because they render exo-/endocytosis-associated pH changes to fluorescent si

155 ar cells was not affected by manipulation of exo-/endocytosis.

156 ns produced in vivo by the wild-type (Pol(+) Exo(+)) enzyme, an exonuclease-deficient mutator variant

157 incorporation of C versus G as the wild-type exo(-) enzyme; however, the k(pol)/K(d) ratio for G vers

158 onal spectra of the Pol(+) Exo(-) and Pol(+) Exo(+) enzymes revealed the patterns and efficiencies of

159 RB69 gp43s lacking proofreading function (Exo(-) enzymes) and/or substituted with alanine, serine,

160 lease domain of DNA polymerase epsilon (POLE-exo*) exhibit a novel mutator phenotype, with markedly e

161 Lastly, PolB1 exo- exhibited a low processivity value of 15, thereby s

162 own to possess 5'-flap endo- (FEN) and 5'-3' exo- (EXO) nuclease activities.

163 on with isogenic mutants secreting wild-type ExoS, ExoS defective in GTPase-activating protein (GAP)

164 fluence on the activity of the GAP domain of ExoS (ExoS-GAP).

165 ur different effector proteins, exoenzyme S (ExoS), ExoT, ExoU, and ExoY.

166 Similar to ExoS, ExoT and ExoY, ExoU requires either a eukaryotic-s

167 the three known type III effector molecules (exoS, exoT, and exoY) in P. aeruginosa PAO1 and assayed

168 ruginosa strain PAO1 or PAK, which expresses ExoS, ExoT, and ExoY, but not ExoU.

169 The impact in mortality of TTSS genotypes (exoS, exoT, exoU, and exoY genes) and resistance profile

170 ur known effector proteins of P. aeruginosa (ExoS, ExoT, ExoU, and ExoY) in wax moth killing.

171 xotoxin A (ETA) and the type III cytotoxins (ExoS, ExoT, ExoU, and ExoY).

172 nucleotide polymorphisms in four toxin genes-exoS, exoT, exoU, and exoY-of the Pseudomonas aeruginosa

173 xpression of the type III secretory proteins ExoS, ExoT, ExoU, or PcrV.

174 ExoS, ExoT, ExoY, and ExoU all possess enzymatic activit

175 PAO1 strains with mutations in exoS, exoT, exoY, or combinations thereof were compared

176 we determined which of the three effectors (ExoS, ExoT, or ExoY) were required for bleb niche format

177 n addition, also detected were antibodies to ExoS, ExoU, or ExoS and ExoU, the latter indicating sequ

178 than methyl iodide approach from the convex (exo) face.

179 , a 14-3-3 family protein, factor-activating ExoS (FAS) had no influence on the activity of the GAP d

180 is minimal, as defined by the ratio of the k(exo) for correctly base-paired DNA divided by the rate o

181 plementary and noncomplementary dNTPs by the exo(-) form of RB69 pol and its mutants.

182 The Klenow exo- fragment of Escherichia coli DNA polymerase I incor

183 ty, we isolated small microparticles, mainly EXO, from mouse insulinoma and examined their activities

184 nity (VDR-BVs) using a high-resolution (ChIP-exo) genome-wide analysis of 27 HapMap lymphoblastoid ce

185 One of the most commonly expressed toxins, ExoS, has two domains that are predicted to affect cytos

186 e of the wild-type or exonuclease-deficient (exo(-)) HSV-1 DNA polymerase (pol).

187 containing 5' flaps with either wild-type or exo(-) HSV-1 pol and Fen-1 led to the efficient producti

188 ficant strand displacement was observed with exo(-) HSV-1 pol.

189 hat are not susceptible to MMR, exonuclease (Exo)-I, ExoX, and RecJ exonuclease redundantly inhibit d

190 5Q) within the highly conserved exonuclease (Exo) III domain.

191 olymerase from bacteriophage RB69 (RB69 gp43 exo-) illustrated these properties, showing that the pol

192 enow fragment of Escherichia coli DNA Pol I (exo-) in single-nucleotide insertions.

193 incorporation experiments indicated that Kf exo(-) incorporates all four nucleotides opposite M(1)dG

194 1, was inhibited by bacterially translocated ExoS, indicating an effect of ExoS on cellular RalA func

195 Conclusions- D-EXO, injected intramurally, substantially inhibited form

196 at are consistent with the Type II (N(cyt)/C(exo)) integral membrane protein topology, and extended h

197 richia coli DNA polymerase I Klenow Fragment exo(-) is much less effective in expanding repeats, beca

198 The identity of this TS structure (syn/exo) is in accord with experimentally determined KIE val

199 Exoenzyme S (ExoS) is a bifunctional Pa TTS effector protein, with GT

200 Exoenzyme S (ExoS) is a bifunctional toxin directly translocated into

201 Pseudomonas aeruginosa Exoenzyme S (ExoS) is a bifunctional type-III cytotoxin.

202 Exoenzyme S (ExoS) is a bifunctional virulence factor directly transl

203 Pseudomonas aeruginosa exoenzyme S (ExoS) is a type III secretion (TTS) effector, which incl

204 Exoenzyme S (ExoS) is translocated into eukaryotic cells by the type

205 med the presence of plasma-derived exosomes (EXOs) isolated by differential centrifugation.

206 methanolysis-rearrangement of (+/-)-6,7-exo,exo-(isopropylidenedioxy)-4-exo-iodo-2-oxabicyclo[3.2.1]

207 not required to observe the pI conformers of ExoS; it enhanced the resolution and formation of pI con

208 olymerase I large (Klenow) fragment, 3'-->5' exo(-) Klenow DNA polymerase, thermophilic Bst DNA polym

209 -mer) DNA and their reactions with the 3'-5' exo(-) Klenow fragment of DNA polymerase I demonstrate t

210 d for translesion synthesis catalyzed by the exo(-) Klenow fragment, the expected three-base deletion

211 The nucleotide preference of POLE-exo* leads to increased frequencies of recurrent nonsens

212 High-resolution exonuclease (ChIP-exo) mapping revealed that the majority of enhancers est

213 omatin immunoprecipitation-exonuclease (ChIP-exo) method allowed the identification of a previously u

214 ation patterns arise from some of these POLE-exo* mutants during genome duplication.

215 xonuclease-deficient mutator variant (Pol(+) Exo(-)), mutator variants with substitutions at Tyr(567)

216 on expected from combining the pol L561A and exo(-) mutator activities.

217 4)-benzoylated 6'-F-tc-(5Me)C reavealed a 2'-exo (north) conformation of the furanose ring, character

218 r a 2'-endo, 3'-exo (South), or a 3'-endo,2'-exo (North) conformation.

219 ADP is a type III bitopic N(endo)C(exo) nuclear membrane and Golgi glycoprotein that is pro

220 possess 5'-flap endo- (FEN) and 5'-3' exo- (EXO) nuclease activities.

221 epted as a substrate by the Klenow fragment (exo(-)) of DNA polymerase I from E. coli.

222 extension studies using the Klenow fragment (exo(-)) of Escherichia coli DNA polymerase I demonstrate

223 erase alpha (pol alpha) and Klenow fragment (exo-) of DNA polymerase I (Escherichia coli).

224 The effect of activated monocyte exosomes (Exos) on endothelial cells is unknown.

225 lic pseudosugar locked in either a North (2'-exo) or South (3'-exo) ring pucker.

226 The exosporium spore layer was retained (exo+) or removed by sonication (exo-).

227 otonated isomers are formed (endo/endo, endo/exo, or exo/exo), which differ in the position of the N-

228 The exo- or endo-selectivity of bicyclic scaffolds depends o

229 Microvesicles (MVs), but not exosomes (Exos) or apoptotic bodies (Abs), are the main type of EV

230 nd together they reveal the dominance of the exo- over the endo-anomeric effect.

231 eles with low levels of 5'-->3' exonuclease (exo) overproduced DNA while those with either high or no

232 on the enzyme's five structural domains (N, exo, palm, fingers, and thumb).

233 However, an exonuclease-deficient (exo(-)) pol (D368A) was capable of slow strand displacem

234 er by alternative polymerases pol II, pol II exo(-), pol IV, and pol V was examined.

235 e replication clamp PCNA, both wild-type and exo(-) Pol delta carried out strand displacement synthes

236 ) and processivity of strand displacement by exo(-) pol, the rate was slower than that for gap-fillin

237 he strand displacement activity of the Vent (exo-) polymerase to generate single strand flaps on nick

238 oration followed by primer extension by Vent(exo-) polymerase.

239 catalyzed by Taq, Vent (exo-) and Deep Vent (exo-) polymerases, with 94.4%, 97.5% and 97.5%, respecti

240 ion synthesis experiments showed that pol II exo(-) preferentially incorporates T opposite C4-AP, fol

241 Type III-delivered exoenzyme S (ExoS) preferentially ADP-ribosylated membrane-associated

242 ne of the major type III secreted effectors, ExoS, prevented triggering of type III secretion by bact

243 In contrast, with Kf exo(-), primers extended past M(1)dG contained T opposit

244 , Okazaki fragment maturation with Pol delta-exo(-) proceeded with an increased duration of nick tran

245 Herein, we investigated the effect of exo- Protoporphyrin based SDT (PpIX-SDT) on SAS cells in

246 cells, where Rac1 is ADP-ribosylated by TTS-ExoS, Rac1 was activated and relocalized to the membrane

247 Further genetic studies showed that pol3-exo(-) rad27 double mutants were sensitive to alkylation

248 d generation of double strand breaks in pol3-exo(-) rad27 mutants was suppressed by the overexpressio

249 of putative conformational changes with the exo(-) RB69 pol and its mutants using a primer-template

250 ciency of G versus G incorporation using the exo(-) RB69 pol.

251 Kf exo(+) removed dA, dG, and T opposite M(1)dG and N(2)OPd

252 Internal mismatches were recognized by Kf exo+ resulting in the excision of the correct base pairs

253 cked in either a North (2'-exo) or South (3'-exo) ring pucker.

254 two proteins central to systemic/exogenous (exo)-RNAi pathway: the dsRNA importer, SID-1 and the arg

255 drogen peroxide when oxygen is bound on the "exo" side ("dock-on") of the catalyst, while four-electr

256 camphor in D(2)O, the magnitude of the C5-OH(exo) signal decreases via H/D exchange.

257 H(2)O buffer, H/D exchange causes the C5-OH(exo) signal to reappear during relaxation upon annealing

258 en the polymerization (pol) and exonuclease (exo) sites of DNAPs, even for perfectly complementary P/

259 cytosine sugar pucker is in the C2'-endo-C3'-exo (South conformation), in contrast to the previously

260 rinated sugar rings for either a 2'-endo, 3'-exo (South), or a 3'-endo,2'-exo (North) conformation.

261 ing a two- to threefold-higher response than exo+ spores.

262 induced a strong cytokine response, with the exo- spores eliciting a two- to threefold-higher respons

263 Rrp44 lacking 3'-exonuclease activity (Rrp44-exo) supports growth in S288c-related strains (BY4741).

264 ibution in human airway cells intoxicated by ExoS, -T, and -Y.

265 ene (BP), primarily blocks Sequenase 2.0, an exo(-) T7 DNA polymerase; however, a mismatched dATP can

266 of polymerase stalling of T7 DNA polymerase exo(-) (T7(-)) and HIV-1 reverse transcriptase (RT) duri

267 6)-BzG) was assessed using T7 DNA polymerase exo(-) (T7(-)) and HIV-1 reverse transcriptase (RT).

268 nalysis on a strand-specific paired-end ChIP-exo (termed as ChIP-ePENS) data of FOXA1 in LNCaP cells

269 Residues 51-72 of ExoS (termed the membrane localization domain, MLD) were

270 complementary to another TS structure (anti/exo) that was not detected in the active site by the sam

271 S and YopE share structural homology, unlike ExoS, the intracellular localization of YopE within mamm

272 biochemical properties that allow Pol delta-exo(-) to carry out strand displacement synthesis and di

273 on the ability of lambda exonuclease (lambda-exo) to efficiently digest parental DNA while leaving RN

274 to chromatin immunoprecipitates (termed ChIP-exo) to examine the precise location of 6,045 PICs in Sa

275 omatin immunoprecipitation-exonuclease (ChIP-exo) to resolve the organization of individual histones

276 The switch from 5-exo- to 6-endo-trig selectivity in the radical cyclizati

277 wn to have a dramatic impact on the ratio of exo- to endo-cyclization reactions, with bicyclo[4.1.0]

278 ExoS within T24 cells, but as with wild-type ExoS, translocation was limited in association with disr

279 nown cellular substrates of TTS-translocated ExoS (TTS-ExoS) ADPRT activity include proteins in the R

280 Here, we identify POLE-exo* tumors in numerous cancers and classify them into t

281 it was proposed that P. aeruginosa secreting ExoS, upon infection, shuts down host cell survival sign

282 ructural data suggest how the preference for exo- versus endo-cleavage mode is linked with recognitio

283 e Klenow fragment of Escherichia coli Pol I (exo(-)) was investigated.

284 Pol II exo(-) was most efficient.

285 rigid (S)-conformation (P = 126 degrees , 1'-exo) was consistent with molecular modeling.

286 nucleotide incorporation catalyzed by PolB1 exo- was limited by a protein conformational change whic

287 ation of an "induced fit" mechanism by PolB1 exo- was supported by the following: a small, alpha-thio

288 or the membrane localization domain (MLD) of ExoS, was required to elicit this phenotype.

289 Using ChIP-exo, we identified the subnucleosomal placement of 20 of

290 With the single bp accuracy provided by ChIP-exo, we show an unprecedented view into genome-wide bind

291 gate whether primary islet cells can produce EXOs, we isolated cells from the islet of Langerhans of

292 Recently, MSC-derived exosomes (MSC-Exo) were thought to carry functions of MSCs.

293 The S-cdG deoxyribose exhibited the O4'-exo (west) pseudorotation.

294 Here we describe ChIP-exo, where an exonuclease trims ChIP DNA to a precise di

295 observed for the ADP-ribosylation of Ras by ExoS, where ADP-ribosylated Ras loses the ability to bin

296 in was determined to be auto-ADP-ribosylated ExoS, whereas the 25-kDa protein appeared to represent a

297 mers are formed (endo/endo, endo/exo, or exo/exo), which differ in the position of the N-H bond's wit

298 bese mice secrete miRNA-containing exosomes (Exos), which cause glucose intolerance and insulin resis

299 c cells, and 11 of 33 had antibodies against ExoS, while most CF sera contained antibodies against Po

300 ulate synaptic endocytosis and hence couples exo- with endocytosis.