ライフサイエンスコーパス: exoglycosidase

1 the N-glycopeptide pentasaccharide core with exoglycosidases.

2 trait that may be shared by other lysosomal exoglycosidases.

3 the glycoconjugate substrate by pneumococcal exoglycosidases.

4 ed glycopeptides were digested stepwise with exoglycosidases.

5 eptide and its partially trimmed products by exoglycosidases.

6 Three exoglycosidases, a neuraminidase (NanA), beta-galactosid

7 The absence of bacterial exoglycosidase activities and paucimannosidic N-glycans

8 mer turnover is PARG, which possesses mainly exoglycosidase activity but can remove olig(ADP-ribose)

9 anced by treatment of the G protein with the exoglycosidase alpha-mannosidase and reduced after subse

10 otal oligosaccharide pool using MALDI/MS and exoglycosidase analysis revealed 24 lactosamine species

11 ation of singular oligosaccharides to define exoglycosidase and glycosyl transferase branch specifici

12 e of oligosaccharides is to digest them with exoglycosidases and analyze the resulting digestion prod

13 Digestion of the glycopeptide by a exoglycosidase array and subsequent mass spectrometric a

14 e and linkage information, were confirmed by exoglycosidase array digestions of aliquots of the N-gly

15 may then be sequenced simultaneously, using exoglycosidase arrays.

16 butions of the endoglycosidase HYAL1 and the exoglycosidase beta-hexosaminidase to the lysosomal degr

17 onjunction with two other surface-associated exoglycosidases, BgaA, a beta-galactosidase, and StrH, a

18 However, we propose that the exoglycosidase-dependent liberation of monosaccharides f

19 Here we have used exoglycosidase digestion and mass spectrometry to sequen

20 ogonal and complementary techniques, such as exoglycosidase digestion arrays, analytical/preparative

21 specific enzymes are also routinely used for exoglycosidase digestion based carbohydrate sequencing.

22 sted to the proper pH can replace the normal exoglycosidase digestion buffers.

23 Exoglycosidase digestion coupled with matrix-assisted la

24 lysis, mild methanolysis, immunoblotting and exoglycosidase digestion indicated that the majority of

25 Using exoglycosidase digestion of the pSTf glycan, we demonstr

26 In exoglycosidase digestion studies, treatment with sialida

27 Using exoglycosidase digestion studies, we have now determined

28 Exoglycosidase digestion was used to identify the indivi

29 In combination with HPLC fractionation, exoglycosidase digestion, and fragmentation of the glyca

30 y multidimensional mass spectrometry, and by exoglycosidase digestion, revealing a predominance of hi

31 a(1-6) arms) were separated and confirmed by exoglycosidase digestion.

32 ass spectrometry (MS/MS) in combination with exoglycosidase digestion.

33 ESI-MS and -MS/CID-MS, linkage analysis, and exoglycosidase digestion.

34 Sequential exoglycosidase digestions of IgA1 allowed identification

35 Exoglycosidase digestions of the glycopeptide from norma

36 We demonstrate new conditions that permit exoglycosidase digestions to be performed on the MALDI t

37 ion/ionisation (MALDI) mass spectrometry and exoglycosidase digestions to dissect the detailed struct

38 utoGU) that progressively analyzes data from exoglycosidase digestions to produce a refined list of f

39 Through exoglycosidase digestions we determined that the O-linke

40 njunction with mass composition analysis and exoglycosidase digestions), Edman degradation, and monos

41 Based on substrate analogue studies, exoglycosidase digestions, and co-chromatography with fu

42 fied by a combination of endoglycosidase and exoglycosidase digestions, anion-exchange chromatography

43 HPLC peak and, when used in combination with exoglycosidase digestions, progressively assigns each st

44 ce liquid chromatography (HPLC) coupled with exoglycosidase digestions.

45 ructures together with predicted products of exoglycosidase digestions.

46 Native virus deglycosylation by endo- and exoglycosidases dramatically reduced cytokine production

47 enhance the stability and performance of the exoglycosidase enzyme neuraminidase and are used to crea

48 The following treatment with an array of exoglycosidase enzymes enables sequencing and a linkage-

49 te sequencing approach using the appropriate exoglycosidase enzymes in conjunction with the utilizati

50 s also utilized for accurate delivery of the exoglycosidase enzymes.

51 ate residues from exposure and hydrolysis by exoglycosidases (galactosidase and hexosaminidase).

52 that SpxR also positively regulates the strH exoglycosidase gene, which, like spxB, has been implicat

53 heir own or combined with mutations in other exoglycosidase genes, resulted in the accumulation of pa

54 okes the activity of both hyaluronidases and exoglycosidases has been advanced.

55 hanges in lectin binding upon treatment with exoglycosidases identified the primary specificities and

56 catalyzed by the concerted action of several exoglycosidases, including a broad specificity lysosomal

57 m mass spectrometry, structure homology, and exoglycosidases is described that allows the structural

58 action was further digested with an array of exoglycosidase mixtures, and subsequent MALDI TOF MS ana

59 Despite these findings, triple exoglycosidase mutants colonized mice as well as their p

60 ry cleavage of terminal glycan residues with exoglycosidases offers a number of advantages over bench

61 eptococcal pathogens have evolved to express exoglycosidases, one of which is BgaC beta-galactosidase

62 try before and after treatment with endo- or exoglycosidases or with alkaline phosphatase.

63 ments with combinations of exosulfatases and exoglycosidases permits the selective removal of specifi

64 phy-mass spectrometry combined with specific exoglycosidase reactions to determine the sequences of N

65 eriments using human serum showed that these exoglycosidases reduced deposition of complement compone

66 ing that overexpression could not bypass the exoglycosidase restriction.

67 ntly, we show that many of the commonly used exoglycosidases retain both their activity and their spe

68 embryos, mass spectrometry fragmentation and exoglycosidase sensitivity defined a novel glucuronyl tr

69 d by lectin binding, saccharide composition, exoglycosidase sensitivity, and NMR spectroscopy.

70 ser-desorption ionisation mass spectrometry, exoglycosidase sequencing combined with normal-phase HPL

71 By use of exoglycosidase sequencing, in conjunction with high-perf

72 he complete structures were obtained through exoglycosidase sequencing.

73 (alpha-NAGAL; E.C. 3.2.1.49) is a lysosomal exoglycosidase that cleaves terminal alpha-N-acetylgalac

74 Alpha-L-fucosidase (ALF) is an exoglycosidase that is involved in the hydrolytic degrad

75 Xyloglucan can be degraded by a suite of exoglycosidases that remove specific sugars.

76 ide standards were digested with one or more exoglycosidases to show that the enzymes retain their ac

77 in small microarrays (2.2 mm x 2.2 mm) with exoglycosidases to successively expose underlying featur

78 E- and PAE-generated fragments of native and exoglycosidase-treated blood-derived CBG of healthy indi

79 dated by LC/MS analysis following sequential exoglycosidase treatments of the endoproteinase Lys-C di

80 defined GBPs and antibodies before and after exoglycosidase treatments on the microarray.

81 bardment (FAB) mass spectrometry, sequential exoglycosidase treatments, methylation analysis, and (1)

82 e glycan antennae were shortened by stepwise exoglycosidase treatments; this trend was consistent reg

83 of sugar residues from the terminal ends by exoglycosidases, up to 50% of total carbohydrates, did n

84 By using specific exoglycosidases, we demonstrated that the SBA binding pr

85 In this way, the exoglycosidases were more effectively used, and their us

86 related glycoforms did not occur naturally, exoglycosidases were used to achieve stepwise removal of

87 Exoglycosidases were used to remove terminally-exposed N