ライフサイエンスコーパス: exon inclusion

1 ng Nf1 exon 23a were manipulated to increase exon inclusion.

2 s with G-quadruplex-forming capacity promote exon inclusion.

3 ping, and a reduction in PTB increased alpha-exon inclusion.

4 tisense oligonucleotide also increased alpha-exon inclusion.

5 T MSE and positively regulates MSE-dependent exon inclusion.

6 f SRp40 in L6 cells mimicked insulin-induced exon inclusion.

7 SEs) are important cis elements required for exon inclusion.

8 -affinity site for U2AF65 strongly inhibited exon inclusion.

9 ternative exon 5 and promote muscle-specific exon inclusion.

10 t of a complex that regulates MSE3-dependent exon inclusion.

11 nce that was specifically required for alpha-exon inclusion.

12 the exon were found to be required for alpha-exon inclusion.

13 element alone can at least partially support exon inclusion.

14 ive exon were sufficient for muscle-specific exon inclusion.

15 he native repeats act to enhance alternative exon inclusion.

16 me to select for exon sequences that enhance exon inclusion.

17 o coordinate splice site pairing and enforce exon inclusion.

18 ly 350 base pairs resulted in enhanced alpha-exon inclusion.

19 icing enhancer (ESE) in exon 3 that promotes exon inclusion.

20 to mediate the embryonic splicing pattern of exon inclusion.

21 get transcripts, leading to misregulation of exon inclusion.

22 city while keeping G tracts intact abrogates exon inclusion.

23 n removal to ultimately activate alternative exon inclusion.

24 ch likely allows splice site recognition and exon inclusion.

25 variable exon and causes increased variable exon inclusion.

26 ts in FD lymphoblast cell lines by improving exon inclusion.

27 del gene and are associated with alternative exon inclusion.

28 the enhancer, hnRNP L unexpectedly activates exon inclusion.

29 r eliminates the ability of Mbnl1 to promote exon inclusion.

30 D45 splicing regulatory elements and repress exon inclusion.

31 easibility of screening for drugs that alter exon inclusion.

32 enhances Sam68 acetylation and CD44 variant exon inclusion.

33 tive 5' splice site selection or alternative exon inclusion.

34 1 in different cells determine the degree of exon inclusion.

35 to induce exon skipping but also to promote exon inclusion.

36 AY cluster enhanced spliceosome assembly and exon inclusion.

37 eak 5' splice site was capable of repressing exon inclusion.

38 hat augments the enhancer function to ensure exon inclusion.

39 tively active Akt2 kinase promotes PKCbetaII exon inclusion.

40 site on a nuclear splicing protein promoting exon inclusion.

41 tronic elements, resulting in enhancement of exon inclusion.

42 ction of the mutated SRp40 attenuated betaII exon inclusion.

43 splicing minigene revealed defective betaII exon inclusion.

44 ther in vitro or in vivo, leads to increased exon inclusion.

45 Rp55 was also demonstrated to play a role in exon inclusion after the removal of intronic splicing si

46 report, we show that insulin regulates this exon inclusion (alternative splicing) via the phosphatid

47 identify synonymous positions important for exon inclusion, an alignment of organisms filtered based

48 between distant splice sites for control of exon inclusion and allows removal of a 74 kb intron as a

49 (p.D107D), has a similar molecular defect of exon inclusion and causes X-linked mental retardation He

50 eted for SMAR1 showed increased CD44 variant exon inclusion and concomitant metastatic propensity, co

51 We found that SRSF10 promoted both exon inclusion and exclusion.

52 other switches these fluorescent products of exon inclusion and exclusion.

53 Both exon inclusion and exon skipping were found to post-tran

54 nd alternative splicing, frequently altering exon inclusion and intron retention in ways not predicte

55 y of this sequence is sufficient for in vivo exon inclusion and is the binding site for the known bri

56 h hnRNP H and hnRNP F as being repressors of exon inclusion and suggest that Fox proteins enhance the

57 of an optimal splice site does not guarantee exon inclusion and that the best predictor for exon reco

58 ximal region of exon 2 that facilitates both exon inclusion and Vif expression.

59 e preserve protein reading-frame in both the exon-inclusion and exon-skip splice forms.

60 coding the adhesion receptor CD44, promoting exon inclusion, and decreasing the overall level of CD44

61 h LY294002 blocked insulin-induced PKCbetaII exon inclusion as well as phosphorylation of SRp40.

62 nic intermediate methylation correlates with exon inclusion at a level between that of fully methylat

63 cores were shown to correlate with levels of exon inclusion, both in vitro and in vivo.

64 demonstrated that SRp20 and SF2/ASF increase exon inclusion but that CUG-BP1 causes exon skipping.

65 Our results suggest that CUGBP2 promotes exon inclusion by a novel mechanism in which CUGBP2 dire

66 amily of proteins has been shown to activate exon inclusion by binding intronic G triplets.

67 racterized RNA binding proteins that promote exon inclusion by binding to exonic splicing enhancers (

68 We conclude that CUGBP2 activates exon inclusion by forming direct interactions with compo

69 the first example of positive regulation of exon inclusion by PTB.

70 es suggest that accurate regulation of micro-exon inclusion by RBFOX proteins and PTBP1 plays an impo

71 cent divergent domain activate MSE-dependent exon inclusion demonstrating an unusual functional redun

72 Increased exon inclusion depends on the number, sequence and chemi

73 vents, including 3'UTR extension, as well as exon inclusion/exclusion impacting on protein kinase and

74 on use in the microarray data, clustering of exon inclusion/exclusion patterns across all Immunologic

75 m or downstream ISS elements increased alpha-exon inclusion from 10% up to 70% in vivo.

76 and PCBP1-dependent alternative splicing and exon inclusion from a CD44 minigene.

77 onic element of MAG and modulate alternative exon inclusion from a MAG minigene reporter.

78 used to characterize splicing enhancers for exon inclusion from a pool of beta-globin-based three ex

79 arks characteristic of active promoters, and exon inclusion in a small but well-defined class of exon

80 ernative 5' splicing also enhance (suppress) exon inclusion in alternative 3' or cassette exon splici

81 Intriguingly, motifs that enhance (suppress) exon inclusion in alternative 5' splicing also enhance (

82 n of RNPS1 with p54 cooperatively stimulated exon inclusion in an ATP synthase gamma-subunit pre-mRNA

83 T that are both necessary and sufficient for exon inclusion in embryonic muscle.

84 rection of FGFR1 gene splicing to >90% alpha-exon inclusion in glioblastoma cells had no discernable

85 rs to monitor the combinatorial diversity of exon inclusion in individual transcripts.

86 c splicing enhancer (ESE) required for alpha-exon inclusion in JEG3 cells.

87 ions in these elements prevent activation of exon inclusion in muscle cells but do not affect the def

88 (ESE), which together determine the level of exon inclusion in naive cells.

89 sites that bind nSR100 to potently activate exon inclusion in neural cells while weakening 3' splice

90 cells but do not affect the default level of exon inclusion in nonmuscle cells.

91 SR proteins are well known to promote exon inclusion in regulated splicing through exonic spli

92 t other SR proteins, directly mediated EIIIB exon inclusion in the fibronectin transcript.

93 K1) is a regulator of Tra2beta1 and promotes exon inclusion in the survival motor neuron gene 2 (SMN2

94 Recombinant hnRNP-L functions to repress exon inclusion in vitro in an ESS1-dependent manner.

95 he binding of U2AF eliminated enhancement of exon inclusion in vivo and exon polyadenylation in vitro

96 onin T (cTNT) alternative exon 5 and promote exon inclusion in vivo and in vitro.

97 combinant PurH with functional activation of exon inclusion in vivo.

98 ancer pyrimidine tract is functional in that exon inclusion increases when in vivo levels of PTB incr

99 ous nuclear ribonucleoprotein A1 facilitates exon inclusion, increasing this ratio.

100 ntarity using compensatory mutations rescues exon inclusion, indicating that the elements act through

101 text with other sequence elements created by exon inclusion involved in affecting mRNA stability.

102 hese results suggest that although the alpha-exon inclusion is actively repressed in glioblastomas, t

103 ty of Nova to enhance or repress alternative exon inclusion is dependent on the position of the Nova-

104 he mechanism of MBNL1-mediated activation of exon inclusion is unknown.

105 are high in Nf1 23aIN/23aIN cells, where the exon inclusion level remains high, but Ras activation is

106 utionarily optimized to support a particular exon inclusion level.

107 became stronger as a function of decreasing exon inclusion level: for alternatively spliced exons th

108 NA variants produced smaller fluctuations of exon inclusion levels than random exonic substitutions,

109 e introduce SpliceTrap, a method to quantify exon inclusion levels using paired-end RNA-seq data.

110 e, and the purine-rich element also supports exon inclusion mediated through the downstream 5' splice

111 selection for in vitro splicing via internal exon inclusion, new consensus motifs and score matrices

112 gnificantly, insulin regulation of PKCbetaII exon inclusion occurred in the absence of cell growth an

113 CELF6 activates exon inclusion of a cardiac troponin T minigene in trans

114 purine residues were critical for repressing exon inclusion of a chimeric splicing reporter.

115 five of these proteins specifically activate exon inclusion of cardiac troponin T minigenes in vivo v

116 ng RNAs in vitro and activated MSE-dependent exon inclusion of cTNT minigenes in vivo.

117 There was a 6- to 14-fold decrease in exon inclusion on ablation of SRp55.

118 binatorial SREs which may be responsible for exon inclusion or exclusion across tissues.

119 Thus in most cases the pattern of exon inclusion or exclusion correlated with stronger or

120 and 3' untranslated regions (UTRs), promotes exon inclusion or exclusion in a context-dependent manne

121 an RNA-binding protein that promotes either exon inclusion or exclusion.

122 as preneoplasias exhibited a more restricted exon inclusion pattern.

123 a developmentally regulated switch such that exon inclusion predominates in embryonic, but not adult,

124 rence, that it is involved in the higher EDA exon inclusion rate in endometrium.

125 In particular, we observed changes in exon inclusion rates and increased presence of intronic

126 m and gene expression as well as the derived exon inclusion rates.

127 w that BRIE yields reproducible estimates of exon inclusion ratios in single cells and provides an ef

128 ecially for accurate quantification of local exon-inclusion ratios from RNA-seq data.

129 y, robustness and reliability in quantifying exon-inclusion ratios.

130 Because SRSF2 promotes exon inclusion, reduced SRSF2 expression would mean that

131 mutations are observed to occur, none of the exon inclusion reducing mutants was found in the filtere

132 cific splicing enhancers (MSEs) that promote exon inclusion specifically in embryonic striated muscle

133 ynonymous mutations across the exon decrease exon inclusion, suggesting that nucleotide identity acro

134 fluorescent reporter for cellular assays of exon inclusion that can accommodate nearly any cassette

135 re that the selected enhancer motif promotes exon inclusion through specific interaction with SRp30.

136 midines in the ESE resulted in a switch from exon inclusion to exon skipping in vivo and abolished bi

137 ound hatching coincided with the switch from exon inclusion to exon skipping, suggesting that loss of

138 We demonstrate that Wt1 promotes exon inclusion using a Vegf-a minigene-based splicing as

139 and nonneuronal cell lines, and the level of exon inclusion was measured by primer extension.

140 Increased exon inclusion was not sequence-specific as exon skippin

141 identify substances that promote alternative exons inclusion, we set up a high-throughput screen and

142 Insulin effects on exon inclusion were observed as early as 15 min after in

143 ose to the 5' splice site generally promoted exon inclusion, whereas binding near the 3' splice site

144 small nuclear ribonucleoprotein) binding and exon inclusion, whereas Nova binding to an intronic YCAY

145 inding to cassette exons was associated with exon inclusion, whereas the binding of SRSF10 within dow

146 major role in maintaining normal FGFR1 alpha-exon inclusion, which is subject to dominant intronic sp

147 redicted Nova's effect to inhibit or enhance exon inclusion, which led us to examine the relationship

148 sufficient for Nova-dependent enhancement of exon inclusion, which we term the NISE (Nova-dependent i

149 tified pyrvinium pamoate as a drug-promoting exon inclusion without editing.

150 ation of the branchpoint caused loss of mini-exon inclusion without loss of splicing enhancement, sho