ライフサイエンスコーパス: exonuclease III

1 ally removed with uracil DNA glycosylase and exonuclease III.

2 from different species and a comparison with exonuclease III.

3 ately 100-fold less efficient than repair by exonuclease III.

4 edly, they alter the cleavage specificity of exonuclease III.

5 scherichia coli DNA polymerase I, and E.coli exonuclease III.

6 phodiesters were protected from digestion by exonuclease III.

7 '-5' linkage itself was poorly hydrolyzed by exonuclease III.

8 ded double strand, which is then digested by exonuclease III.

9 to the location of the extra alpha-helix in exonuclease III.

10 the metal ion dependence of Escherichia coli exonuclease III, 3'-5'-exonuclease and exoribonuclease H

11 y enhanced the dCTPalphaB resistance towards exonuclease III (5-Et-dCTPalphaB >5-Me-dCTPalphaB >dCTPa

12 DNase I is structurally homologous to exonuclease III, a DNA-repair enzyme with multiple activ

13 III-inactive protruding 3' terminus into an exonuclease III-active blunt end, triggering the digesti

14 sensitive, and reproducible method based on Exonuclease III-aided target recycling technique applied

15 acids, comprising the 10 amino acids of the exonuclease III alpha-helix flanked by a glycine rich re

16 Therefore, the novel Exonuclease III-amplified flow cytometry bead assay has

17 lethality caused by a combined deficiency of exonuclease III and dUTPase, which has been attributed t

18 Exonuclease III and endonuclease IV are the major enzyme

19 tant cells lack activity at the positions of exonuclease III and endonuclease IV but retain activity

20 C2-AP is incised less efficiently by exonuclease III and endonuclease IV than are other abasi

21 Incision of L and C4-AP lesions by exonuclease III and endonuclease IV was determined under

22 two Escherichia coli 3'-phosphodiesterases, exonuclease III and endonuclease IV, are readily detecte

23 cleavage when two Escherichia coli enzymes, exonuclease III and endonuclease IV, are used.

24 , uracil DNA glycosylase-deficient, ung-, or exonuclease III and endonuclease IV-deficient, xth-nfo-)

25 bed using Escherichia coli AP endonucleases, exonuclease III and endonuclease IV.

26 repair proteins, including Escherichia coli exonuclease III and human APE, that repair oxidative and

27 bstrates by micrococcal nuclease mapping and exonuclease III and hydroxyl radical footprinting reveal

28 ization of the structure of AQ-157TG NCPs by Exonuclease III and hydroxyl radical footprinting, we co

29 igestion differs significantly between WRNp, exonuclease III and Klenow, indicating that each exonucl

30 ects of these linkages on the dsDNA specific exonuclease III and on the ssDNA specific mung bean nucl

31 ootprint of RNAP II arrested at a CPD, using exonuclease III and T4 DNA polymerase's 3'-->5' exonucle

32 ncipal replicative helicases or proofreading exonucleases; (iii) apparently orthologous but poorly co

33 rocessing enzymes such as endonuclease IV or exonuclease III are not absolutely required for repair o

34 ins (deficient in the major AP endonuclease, exonuclease III) are sensitive.

35 nzymes, such as Escherichia coli RNase H and exonuclease III, are known to use metal cofactors in the

36 Here we use T7 RNA polymerase and exonuclease III as probes to obtain a more relevant lowe

37 lution, with sensing signals amplified by an exonuclease III-based target recycling strategy.

38 ical changes, in situ DNA end labeling, post-exonuclease III BrdUrd labeling, and DNA fragmentation.

39 eavage at abasic sites in DNA, a property of exonuclease III but not native DNase I.

40 Introduction of target sequence induces the exonuclease III catalyzed probe digestion and generation

41 The results presented here show that exonuclease III degrades single-stranded DNA as a substr

42 increases protection of nucleosomal DNA from exonuclease III digestion by approximately 10 bp.

43 s demonstrated using a strategy that employs exonuclease III digestion of a target sequence.

44 Minor exonuclease III digestion products in this substrate ind

45 ong with removal of unligated genomic DNA by exonuclease III digestion.

46 ng sequence of the 24K gene was localized by exonuclease III digestion.

47 uctures by hydroxyl radical footprinting and exonuclease III digestion.

48 tails extending from the ends as assessed by exonuclease III digestion.

49 h DNase I and 1,10-phenanthroline-copper and exonuclease III digestions showed that ecteinascidin 743

50 ns as revealed by footprinting studies using exonuclease III, DNase I, and hydroxyl radical.

51 Exonuclease III, encoded by the xthA gene, plays a centr

52 In this work, we exploit exonuclease III (Exo III) activity on DNA hybrids contai

53 leic acid self-assembly circuitry and enzyme exonuclease III (Exo III) for the differentiation of sin

54 The enzyme Exonuclease III (Exo III) is a useful tool in this regar

55 ified optical aptasensor system based on the Exonuclease III (Exo III) recycling of the VEGF analyte

56 Escherichia coli exonuclease III (Exo III) removes apyrimidinic or apurin

57 AP endonucleases endonuclease IV (Endo IV), exonuclease III (Exo III), and Ape1 on the reaction kine

58 of homology to its prokaryotic counterpart, exonuclease III (Exo III), except for the amino terminus

59 ive detection of streptomycin (STR) based on Exonuclease III (Exo III), SYBR Gold and aptamer complim

60 multifunctional dumbbell probe can initiate exonuclease III (Exo III)-aided target recycling amplifi

61 Herein, we report a type of exonuclease III (Exo III)-powered stochastic DNA walker

62 -amino-acid domain of SNIP is related to the exonuclease III (ExoIII) domain of the 3'-->5' proofread

63 We obtained exonuclease III (exoIII) footprints for a series of RNA

64 und that a combined treatment with MNase and exonuclease III (exoIII) overcomes MNase sequence prefer

65 ' end-resection reaction of Escherichia coli exonuclease III (ExoIII), a DNA repair enzyme.

66 which shares homology with Escherichia coli exonuclease III (ExoIII), is the major abasic endonuclea

67 , digests 5'-3', as well as Escherichia coli exonuclease III (ExoIII), which digests 3'-5', could sub

68 We have developed a novel DNA assay based on exonuclease III (ExoIII)-induced target recycling and th

69 e DNase I and its Escherichia coli homologue exonuclease III (EXOIII).

70 roteins form a distinct subfamily within the exonuclease III (ExoIII)/Ape1/Apn2 family of proteins.

71 CCR4, unlike all other exonuclease III family members, contains a leucine-rich

72 in vitro suggests that this property of the exonuclease III family of AP endonucleases is remarkably

73 homology to the major human/Escherichia coli exonuclease III family of AP endonucleases.

74 with a 347-residue segment homologous to the exonuclease III family of AP endonucleases.

75 trations, and is a specific inhibitor of the exonuclease III family of enzymes to which APE1 belongs.

76 CCR4, a poly(A) deadenylase of the exonuclease III family, is a component of the multiprote

77 s with a general view in the literature that exonuclease III favors double-stranded nucleic acid as a

78 The size of the exonuclease III footprint of ligase bound a single nick

79 e have examined the DNA binding affinity and exonuclease III footprint of the EcoKI type IA restricti

80 Finally, as assessed by exonuclease III footprinting and transcription profiles,

81 Exonuclease III footprinting of the (S3/HsdM)(2) -DNA co

82 In vivo exonuclease III footprinting showed that treatment with

83 DNase I, micrococcal nuclease, and exonuclease III footprinting suggests that UBF and histo

84 n resistance, lifetime, DNAseI footprinting, exonuclease III footprinting, permanganate footprinting

85 Exonuclease III footprints of the arrested complexes are

86 at low pH may be similar to the activity of exonuclease III from E. coli.

87 In an xthA (exonuclease III gene) mutant where there are 3-fold more

88 Catalytic activation of E. coli exonuclease III has been examined for a series of inert

89 res extensive homology with Escherichia coli exonuclease III, has nuclease activity, and provides res

90 Experimental conditions for exonuclease III have been optimized for this application

91 nd phosphatase activities of Esherichia coli exonuclease III have been quantitatively measured, offer

92 referential exodeoxyribonuclease activity of exonuclease III in combination with the difference in di

93 cribes a novel approach utilizing the enzyme exonuclease III in conjunction with 3'-terminated DNA mi

94 could also be obtained with purified E.coli exonuclease III in vitro, but a quantitative comparison

95 the presence of an additional alpha-helix in exonuclease III, in a position suggestive of interaction

96 n with the target DNA transforms the probe's exonuclease III-inactive protruding 3' terminus into an

97 ease-amplified DNA detection scheme in which exonuclease III is used to "recycle" target molecules, t

98 icking endonucleases or sequence independent exonuclease III, lambda exonuclease, RNase H, RNase HII,

99 mplate using a combination of digestion with exonuclease III, lambda exonuclease, RNAse T1, and treat

100 mino acids at Ser174, converts DNase I to an exonuclease III-like enzyme with DNA-repair properties.

101 tigated by hydroxyl radical footprinting and exonuclease III mapping.

102 Exonuclease III mediated in vivo DNA footprinting and di

103 in vitro reconstituted mononucleosomes using exonuclease III, micrococcal nuclease and restriction en

104 using a variety of nuclease probes including exonuclease III, micrococcal nuclease, DNase I, and rest

105 Enzymatic digestion by exonuclease III of the nucleosome substrates revealed th

106 In contrast, yFACT enhanced access to exonuclease III only at very high levels of enzyme, sugg

107 erwise identical DNA sequence was incised by exonuclease III or endonuclease IV approximately 6-fold

108 er significant nuclease resistance to either exonuclease III or mung bean nuclease, but unexpectedly,

109 ichia coli xthA mutants have also shown that exonuclease III participates in the repair of oxidative

110 fer assays are then used in conjunction with exonuclease III protection analysis to investigate the e

111 merase II along a number of templates, using exonuclease III protection as our assay.

112 s, electrophoretic mobility shift assays and exonuclease III protection assays, we localized the basa

113 Exonuclease III protection revealed that CcpA protects a

114 , encoding the ribosomal protein L31 and the exonuclease III, respectively.

115 By mapping the breaks and by studies of the exonuclease III sensitivity of the broken ends, we concl

116 s faster than that recently observed for the exonuclease III surface hydrolysis of double-stranded DN

117 have suggested a role for Asn212 (Asn153 in exonuclease III, the bacterial homologue of HAP1) in sub

118 Exonuclease III, the E. coli homolog of Ape1, did not di

119 re, these results provide constraints on how exonuclease III-thiotriphosphate-polymerase combinations

120 orothioate linkages into DNA, and the use of exonuclease III to determine where those linkages have b

121 oxidized DNA by exploiting Escherichia coli exonuclease III to remove fragments containing direct st

122 units and the DNA could allow more access of exonuclease III to the DNA and account for the shorter f

123 Addition of Exonuclease III to the system allows the recycling of th

124 By using exonuclease III-treated linear duplex DNA with various l

125 DNA resistant to degradation in a subsequent exonuclease III treatment.

126 was excised from a 2D gel and identified as exonuclease III using matrix-assisted laser-desorption i

127 We found that exonuclease III was consistently arrested at positions 1

128 Independent experiments with exonuclease III, which probes the outermost DNA segments

129 method involving simultaneous digestion with exonuclease III, which removes linker DNA.

130 PdG adducts were protected from digestion by exonuclease III, which was consistently arrested at posi

131 rference method to probe the interactions of exonuclease III with the minor groove.

132 e AP endonuclease activities associated with exonuclease III (xth) and endonuclease IV (nfo), indicat

133 We have previously demonstrated that the two Exonuclease III (Xth) family members present within the