ライフサイエンスコーパス: exonucleolytic

1 components of the 60S ribosomal subunit, and exonucleolytic 5' processing of 5.8S rRNA was strongly i

2 unction with various metal ions at the 3'-5' exonucleolytic active site of the Klenow fragment (KF) o

3 e-stranded DNA substrates bound to the 3'-5' exonucleolytic active site of the large fragment of DNA

4 Eukaryotic 3'-->5' exonucleolytic activities are essential for a wide varie

5 MRN catalyzes sequential endonucleolytic and exonucleolytic activities on both 5' and 3' strands of D

6 Degradation by exonucleolytic activities was temperature sensitive in e

7 replication, relying on endonucleolytic and exonucleolytic activities, respectively.

8 ifunctional RNases possessing both endo- and exonucleolytic activities.

9 ease, encoded by the smx gene, that exhibits exonucleolytic activity and is regulated as part of the

10 They possess both 5'-3' exonucleolytic activity and the ability to cleave bifurc

11 activity at branched DNA structures and its exonucleolytic activity at nick and gap structures.

12 al NF-kappaB pathway is essential for normal exonucleolytic activity during human V(D)J recombination

13 merases from the A and B families with 3'-5' exonucleolytic activity have exonuclease domains with si

14 e consequence of endonucleolytic or 5'-to-3' exonucleolytic activity is unclear.

15 A model explaining the exonucleolytic activity of FEN-1 in terms of its endonuc

16 teins define termini by blocking the 5'-->3' exonucleolytic activity of ribonuclease J (RNase J).

17 The assay exploits the 5'-->3' exonucleolytic activity of Taq DNA polymerase to increas

18 eam of either primer during PCR, the 5'-->3' exonucleolytic activity of Taq polymerase degrades it, a

19 The role of the exonucleolytic activity of the calf 5' to 3' exo/endonuc

20 P-1 may play a role in the regulation of the exonucleolytic activity of WRN.

21 hFEN1 endonucleolytic activity versus hEXO1 exonucleolytic activity on 5'-flap substrates.

22 s blocked by a terminal dU; there was slight exonucleolytic activity on a 3'-terminal A or C but no a

23 because reverse transcriptases (RT) lack an exonucleolytic activity that can remove incorporated nuc

24 n DNA polymerase beta, which lacks intrinsic exonucleolytic activity, primer extension proceeds to th

25 a potent 3' to 5' single-strand DNA-specific exonucleolytic activity.

26 lacking the nuclease domain does not display exonucleolytic activity.

27 s of these RNAs and protects them against 3'-exonucleolytic activity.

28 tary nucleotides using Kpom1 lacking 3'-->5' exonucleolytic activity.

29 nation intermediates are substrates for both exonucleolytic and 5' flap endonucleolytic cleavage.

30 Human FEN1 exhibits both a 5' to 3' exonucleolytic and a structure-specific endonucleolytic

31 e demonstrated the presence of both 5'-to-3' exonucleolytic and endonucleolytic activities on the Bac

32 1 is a dual-specificity enzyme, with both 5' exonucleolytic and endonucleolytic activities.

33 alent cation requirements and possessed both exonucleolytic and endonucleolytic functions.

34 n EXO-1 and dramatically stimulates both the exonucleolytic and endonucleolytic incision functions of

35 added to the assay, the balance between the exonucleolytic and endonucleolytic modes of hydrolysis s

36 Exonucleolytic and fork-gap-endonucleolytic reactions we

37 e rRNAs may provide a buffer zone against 3' exonucleolytic attack, thereby preserving the encoded se

38 yadenylate (polyA) tail to sequester it from exonucleolytic attack.

39 tein stimulates both the endonucleolytic and exonucleolytic cleavage activity of FEN-1 and this funct

40 from the primer-templates indicate that the exonucleolytic cleavage activity of Klenow fragment is c

41 substrate for Taq DNA polymerase only after exonucleolytic cleavage had destabilized the hairpin.

42 rophosphorolysis as well as in the endo- and exonucleolytic cleavage of the nascent RNA.

43 th RNA chain extension and RNA shortening by exonucleolytic cleavage or pyrophosphorolysis and increa

44 cular principles governing diverse endo- and exonucleolytic cleavage specificities of members of the

45 h on the ability of HMO2 to protect DNA from exonucleolytic cleavage suggests that more than one HMO2

46 markable ability of HMO2 to protect DNA from exonucleolytic cleavage, combined with reports that HMO2

47 leolytic cleavage are most likely removed by exonucleolytic decay as well, but these events have not

48 ified CspE impedes poly(A)-mediated 3' to 5' exonucleolytic decay by PNPase by interfering with its d

49 lp explain how deadenylation, decapping, and exonucleolytic decay can all be independently activated

50 These data indicate that 3'-5' exonucleolytic decay is the major pathway of RNA degrada

51 enylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body.

52 Furthermore, 3'-5' exonucleolytic decay was stimulated dramatically by AU-r

53 ibonucleotide fragments generated by 3'-->5' exonucleolytic decay, and cleavage of m(7)GDP generated

54 se mRNA decay processes, as well as 5'-to-3' exonucleolytic decay, associate with the protein tristet

55 sitive to structures or sequences that block exonucleolytic decay, is required for efficient decay of

56 resulting in mRNA deadenylation followed by exonucleolytic decay, mRNA endonucleolytic cleavage, or

57 to probe pathways of endonucleolytic versus exonucleolytic decay, were measured in an RNase Y-defici

58 activities are linked and there is a coupled exonucleolytic decay-dependent decapping pathway.

59 some complex is required for efficient 3'-5' exonucleolytic decay.

60 uires deadenylation, decapping, and 5'-to-3' exonucleolytic decay.

61 n precedes decapping and subsequent 5'-to-3' exonucleolytic decay.

62 adenylation followed by 5' end decapping and exonucleolytic decay.

63 d for both 5'-to-3' and 3'-to-5' pathways of exonucleolytic decay.

64 tivate deadenylation, decapping, or 3'-to-5' exonucleolytic decay.

65 volving deadenylation and subsequent 3'-->5' exonucleolytic decay.

66 nylation, followed by decapping and 5' to 3' exonucleolytic decay.

67 provokes rapid decapping followed by 5'-->3' exonucleolytic decay.

68 ly dominant, suggesting that they carried an exonucleolytic defect but retained binding to the pol II

69 ntial service by protecting chromosomes from exonucleolytic degradation and end-to-end fusions and by

70 sistent with protection of the DNA ends from exonucleolytic degradation and repair by the c-NHEJ path

71 modification exposes transcripts to rapid 5' exonucleolytic degradation by RNase J, which is absent i

72 pyrophosphohydrolase RppH triggers rapid 5'-exonucleolytic degradation by RNase J.

73 The P-element termini were protected from exonucleolytic degradation following the cleavage reacti

74 ation is a widespread process in which 5'-3' exonucleolytic degradation follows the last translating

75 adenylation, subjects substrate RNAs to slow exonucleolytic degradation from the 3' end in vitro.

76 tides upstream of the intron insertion site, exonucleolytic degradation is required for recombination

77 Processive 3'-to-5' exonucleolytic degradation of an SP82 phage RNA substrat

78 Nbs1 inhibits Mre11/Rad50-catalyzed 3'-to-5' exonucleolytic degradation of clean DNA ends.

79 Exonucleolytic degradation of DNA is an essential part o

80 caps the cap structure generated by 3' to 5' exonucleolytic degradation of mRNA.

81 f1p, Nmd2p, and Upf3p regulate decapping and exonucleolytic degradation of nonsense-containing mRNAs.

82 The kinetics for exonucleolytic degradation of single-stranded, paired, a

83 RecJ requires interaction with SSB for exonucleolytic degradation of ssDNA but not dsDNA.

84 ivates the mRNA and that this is followed by exonucleolytic degradation of the cleavage products.

85 The resulting exonucleolytic degradation of the primer serves to move

86 n yeasts was shown to be lethal due to rapid exonucleolytic degradation of uncapped transcripts or fa

87 ction in cell extracts, which occurred by 3'-exonucleolytic degradation rather than endonucleolytic f

88 karyotic gene expression and often relies on exonucleolytic degradation to eliminate dysfunctional tr

89 nstream cleavage product, protecting against exonucleolytic degradation, and thereby limiting the ext

90 Since these ends are subject to exonucleolytic degradation, the assay may demand rapid r

91 eep-sequencing method to measure chromosomal exonucleolytic degradation, we demonstrate that the abse

92 ion-dependent decapping followed by 5' to 3' exonucleolytic degradation.

93 pping that exposes the transcript to 5'-->3' exonucleolytic degradation.

94 thereby exposing the mRNA to rapid 5' to 3' exonucleolytic degradation.

95 ff the 5' cap to leave an end susceptible to exonucleolytic degradation.

96 ng, which exposes the transcript to 5' to 3' exonucleolytic degradation.

97 tion that exposes the transcript to 5' to 3' exonucleolytic degradation.

98 ss of deadenylation, decapping and 5' --> 3' exonucleolytic degradation.

99 ng, which exposes the transcript to 5' to 3' exonucleolytic degradation.

100 and protects the mature 3'-end of TER1 from exonucleolytic degradation.

101 ed, antisense transcription-controlled 3'-5' exonucleolytic degradation.

102 elomerase to the 3'-end and protects against exonucleolytic degradation.

103 s induced by DNA topoisomerase II, including exonucleolytic deletion and template-directed polymeriza

104 also participates in RNA degradation through exonucleolytic digestion and polyadenylation.

105 ow that the Ku complex shields DNA ends from exonucleolytic digestion but facilitates endonucleolytic

106 e ability of damaged nucleo-tides to inhibit exonucleolytic digestion differs significantly between W

107 beta signal joints, and from N additions and exonucleolytic digestion for TCR-delta joints.

108 th defective helicase activity are active in exonucleolytic digestion of DNA.

109 ysis of chromosome fusion junctions revealed exonucleolytic digestion of dysfunctional ends prior to

110 hat progresses from removal of the 5' cap to exonucleolytic digestion of the body of the mRNA.

111 eadenylation followed by decapping and 5'-3' exonucleolytic digestion of the mRNA.

112 pre-microRNA/Exp5/Ran-GTP complex inhibited exonucleolytic digestion of the pre-miRNA in vitro.

113 the 5' cap structure (decapping) and 5'->3' exonucleolytic digestion, or by 3' to 5' degradation.

114 sence of RNase A, but not RNase H, inhibited exonucleolytic digestion, suggesting that a ribonucleopr

115 removal of the cap structure permits 5'-->3' exonucleolytic digestion.

116 denylation followed by decapping and 5'-->3' exonucleolytic digestion.

117 decay intermediate is generated by 5'-to-3' exonucleolytic digestion.

118 functional complex and create substrates for exonucleolytic digestion.

119 tion which exposed the transcript to 5'-->3' exonucleolytic digestion.

120 -3': XY = GG, GA, AG, GU) are found to block exonucleolytic digestion.

121 degraded through either 5' to 3' or 3' to 5' exonucleolytic digestion.

122 d in the cytoplasm by decapping and 5'-to-3' exonucleolytic digestion.

123 mbination and protecting genomic DNA against exonucleolytic DNA degradation.

124 berrant recombination and protecting against exonucleolytic DNA degradation.

125 We also provide a genome-wide analysis of exonucleolytic DSB resection lengths and elucidate spati

126 Exonucleolytic editing of incorrectly incorporated nucle

127 Thus, exonucleolytic end resection, rather than chromosome fus

128 n and recruits decapping, deadenylation, and exonucleolytic enzymes to PBs for RNA turnover.

129 and the nuclear exosome, a large complex of exonucleolytic enzymes.

130 ely 50 bp) oligonucleotide substrates during exonucleolytic excision by the formation of a discontinu

131 ucleases (FENs) catalyze endonucleolytic and exonucleolytic (EXO) DNA hydrolyses.

132 on of Rad50 does not significantly alter the exonucleolytic function of Mre11.

133 ole of the PNPase-RNase Y interaction in the exonucleolytic function of PNPase needs to be clarified.

134 e motif selectively eliminated the 3' --> 5' exonucleolytic function of the purified mutant polymeras

135 the high mispair specificity of pol gamma in exonucleolytic hydrolysis is maintained, indicating that

136 Flap endonucleases (FENs) catalyse the exonucleolytic hydrolysis of blunt-ended duplex DNA subs

137 2)P at the 5' or 3' ends, *AN/L3 carried out exonucleolytic hydrolysis of both substrates exclusively

138 eled RNA in a manner consistent with a 3'-5' exonucleolytic mechanism.

139 ning alternate secondary structures, with an exonucleolytic mode of action suggestive of RNaseD.

140 response factor 1, proteins that facilitate exonucleolytic mRNA, to exit SGs.

141 ly(A) tails by what has been described as an exonucleolytic process that can be blocked by the presen

142 ved a coding-partner-dependent difference in exonucleolytic processing and an age-specific difference

143 tional mutagenesis in S.pombe is preceded by exonucleolytic processing and concatomerization of the t

144 P cleavage, while the 3' terminus undergoes exonucleolytic processing by a combination of 3' --> 5'

145 This was followed by 3' to 5' exonucleolytic processing by RRP6 and the exosome, an en

146 the 11-subunit cellular noncoding RNA 3'-5' exonucleolytic processing complex RNA exosome.

147 The subsequent exonucleolytic processing is carried out largely by RNas

148 In addition, the extent of exonucleolytic processing of coding ends was inversely r

149 Our results suggest that exonucleolytic processing of primary DNA lesion by hRAD9

150 Because models predict exonucleolytic processing of the cleaved recipient leadi

151 stream from the mature terminus, followed by exonucleolytic processing to a stem-loop within the 3'-u

152 eterogeneous 5' termini that may result from exonucleolytic processing, and occasionally robust decap

153 nascent transcript and protect it from 3'-5' exonucleolytic processing.

154 ctions, unifying the mechanisms of endo- and exonucleolytic processing.

155 With poly(dA):oligo(dT)50 as substrate, the exonucleolytic products formed a continuous ladder with

156 DNA polymerase beta has no exonucleolytic proof-reading ability, and exhibits high

157 cherichia coli dnaQ gene encodes the 3'-->5' exonucleolytic proofreading (epsilon) subunit of DNA pol

158 ranscriptase (RT) does not possess 3'- to 5'-exonucleolytic proofreading activity and because RT has

159 tes are removed by DNA polymerase-associated exonucleolytic proofreading activity and/or the postrepl

160 (epsilon) subunit of HE provides the 3'-->5' exonucleolytic proofreading activity for this complex.

161 on subunit of the HE complex provides the 3'-exonucleolytic proofreading activity for this enzyme com

162 h repair (MMR) machinery, rather than by the exonucleolytic proofreading activity of DNA polymerase.

163 has been hypothesized to enhance the 3'-->5' exonucleolytic proofreading activity of epsilon.

164 delta variants that harbor or lack 3' --> 5'-exonucleolytic proofreading activity were purified from

165 erichia coli DNA polymerase III possesses 3'-exonucleolytic proofreading activity.

166 Exonucleolytic proofreading and DNA mismatch repair (MMR

167 replication errors that are subject to both exonucleolytic proofreading and dnaE antimutator effects

168 fidelity, even in the absence of its 3'-->5' exonucleolytic proofreading and is significantly more ac

169 he corresponding frameshift intermediates by exonucleolytic proofreading and/or mismatch repair.

170 We suggest that the normal level of exonucleolytic proofreading associated with the mutant P

171 dium, where the mutD5 strain is defective in exonucleolytic proofreading but has a functional MMR sys

172 on by mammalian terminal transferase, blocks exonucleolytic proofreading by Escherichia coli DNA poly

173 Thus, both polymerase selectivity and exonucleolytic proofreading efficiency are diminished du

174 The influence of mutations in the 3' to 5' exonucleolytic proofreading epsilon-subunit of Escherich

175 the polymerase domain is more critical than exonucleolytic proofreading for the fidelity of pol I in

176 d in vivo by mutations that eliminate the 3'-exonucleolytic proofreading function.

177 merase, and the epsilon subunit contains the exonucleolytic proofreading function.

178 ce of these analogs in DNA and inhibition of exonucleolytic proofreading may also contribute to mitoc

179 ort the model that the rate-limiting step in exonucleolytic proofreading of DNA by epsilon subunit is

180 test the hypothesis that the contribution of exonucleolytic proofreading to frameshift fidelity durin

181 ce nucleotide selectivity, the efficiency of exonucleolytic proofreading, and the rate of forming err

182 ns of polymerase base selectivity, 3' --> 5' exonucleolytic proofreading, mismatch correction, and DN

183 results from high nucleotide selectivity and exonucleolytic proofreading.

184 Both effects are classical hallmarks of exonucleolytic proofreading.

185 s are present to counteract the effect of 3'-exonucleolytic proofreading.

186 pe pol gamma despite the action of intrinsic exonucleolytic proofreading.

187 We show for the first time that the exonucleolytic Rat1.Rai1 complex can elicit the release

188 he enzymes that carry out the early 5' to 3' exonucleolytic reactions that generate the mature rRNAs.

189 e snoRNA genes and their processing involves exonucleolytic release of the snoRNA from debranched int

190 he editing site followed by U-specific 3'-5'-exonucleolytic removal of nonbase-paired Us prior to lig

191 e (pol gamma) by comparing the insertion and exonucleolytic removal of six antiviral nucleotide analo

192 e presence of dNTP and to a lesser extent to exonucleolytic removal of the 3'-phosphate-bearing termi

193 inal C (producing a base substitution), (ii) exonucleolytic removal of the C, or (iii), for the G-con

194 DNA-PK, Artemis catalyzed extensive 5'-->3' exonucleolytic resection of double-stranded DNA.

195 o a futile cycle of abortive TLS followed by exonucleolytic reversal.

196 anscriptional RNA cleavage events, and 5'-3' exonucleolytic RNA degradation in the mammalian Pol II t

197 the potential of protocols incorporating an exonucleolytic snake venom phosphodiesterase (SVPD) dige

198 al processing requires a series of endo- and exonucleolytic steps for the production of mature riboso

199 by AGO4 and subsequently subjected to 3'-5' exonucleolytic trimming for maturation.

200 Has1 binding triggers exonucleolytic trimming of 27SA3 pre-rRNA to generate th

201 7p is specifically required for the 5' to 3' exonucleolytic trimming of the 27SA(3) into the 27SB(S)

202 piRNA 3' ends are probably formed by exonucleolytic trimming, after a piRNA precursor is load

203 sors by endonucleolytic cleavage followed by exonucleolytic trimming.

204 B. subtilis, a long-standing question on the exonucleolytic versus endonucleolytic nature of 16S rRNA