ライフサイエンスコーパス: exoribonuclease

1 bonuclease, and Rrp6, a distributive 3'-->5' exoribonuclease.

2 polyribonucleotide polymerase or a 3'-to-5' exoribonuclease.

3 teract with SKI is degraded by 5'-to-3' XRN4 exoribonuclease.

4 degraded by the nuclear exosome and a 5'-3' exoribonuclease.

5 otide phosphorylase (hPNPaseold-35), a 3',5'-exoribonuclease.

6 on, was also shown to be an Mn(2+)-dependent exoribonuclease.

7 subtilis yvaJ gene encodes a second 3'-to-5' exoribonuclease.

8 hich is a subunit of mitochondrial 3' --> 5' exoribonuclease.

9 gen and to Escherichia coli RNase D, a 3'-5' exoribonuclease.

10 ted the nsp10-driven activation of the nsp14 exoribonuclease.

11 al Mtb protein Rv2179c as a highly divergent exoribonuclease.

12 (RppH), allowing access to both endo- and 5' exoribonucleases.

13 hosphorylated RNA that is a substrate for 5' exoribonucleases.

14 erfamily of nonspecific, 3'-->5', processive exoribonucleases.

15 n of the Rrp44p subunit that resembles other exoribonucleases.

16 cessivity factor to assist RNA maturation by exoribonucleases.

17 RNA, we used three Escherichia coli 3' to 5' exoribonucleases.

18 e and phylogenetic distribution of the known exoribonucleases.

19 cells involves the action of both endo- and exoribonucleases.

20 adation in the 5' to 3' direction by the XRN exoribonucleases.

21 y via inhibition of the RNA-processing 5'-3' exoribonucleases.

22 gene regulation that involves both endo- and exoribonucleases.

23 demonstrate that CARF associates with 5'-3' exoribonuclease 2 (XRN2), which plays a major role in bo

24 decrease coincided with an increase in 5'-3'-exoribonuclease 2 protein and alterations in DICER1 and

25 A human 3'-5'-exoribonuclease (3'hExo) has recently been identified an

26 ncode the first known viral RNA proofreading exoribonuclease, a function that likely allowed expansio

27 nt and excess ncRNAs are largely degraded by exoribonucleases, a key characteristic of these RNAs is

28 tory mechanism affecting RNase R, a 3' to 5' exoribonuclease able to act on essentially all RNAs incl

29 family of enzymes, is a 3' to 5' hydrolytic exoribonuclease able to digest highly structured RNA.

30 presence of a 3'-phosphoryl group abolishes exoribonuclease action, it has no effect on the endoribo

31 Rrp6 is inhibited by a mutation in the Rrp44 exoribonuclease active site in 11-subunit nuclear exosom

32 unknown mechanism and compete with 3'-to-5' exoribonuclease activities in hen1.

33 s to the distributive or processive 3' to 5' exoribonuclease activities of Rrp6 or Rrp44, respectivel

34 Two other 3'-5'-exoribonuclease activities were also detected in the mit

35 e their processive and distributive 3'-to-5' exoribonuclease activities, respectively.

36 ns at least three polypeptides with 3' to 5' exoribonuclease activities.

37 yrophosphohydrolase, decapping, and 5'-to-3' exoribonuclease activities.

38 ructural protein 14 (nsp14) encodes 3'-to-5' exoribonuclease activity (ExoN), which performs a proofr

39 nd its human homologs, DIS3 and DIS3L1, have exoribonuclease activity and bind to the core RNA exosom

40 s pyrophosphohydrolase, decapping, and 5'-3' exoribonuclease activity and functions as an important c

41 tein 2 expressed in insect cells has a 3'-5' exoribonuclease activity and was therefore renamed RNA e

42 might be responsible for the acquisition of exoribonuclease activity by RNase BN.

43 nucleotide phosphorylase, the major 3'-to-5' exoribonuclease activity in cell extracts, is viable.

44 estration mechanism for strict control of 3' exoribonuclease activity in the RE complex.

45 analyzed the role of Dcs1 in the control of exoribonuclease activity in vitro and propose that Dcs1

46 ns had reduced or were essentially devoid of exoribonuclease activity in vitro.

47 Finally, a stable structural barrier to exoribonuclease activity inhibited A-site cleavage when

48 ription, whereas the C domain contains 3'-5' exoribonuclease activity involved in suppressing interfe

49 doribonuclease and also demonstrate that its exoribonuclease activity is capable of functioning in vi

50 Rat1 exoribonuclease activity is stimulated by the protein Ra

51 A cytoplasmic 5'-3' exoribonuclease activity is therefore essential for yeas

52 xperimentally that citrate also inhibits the exoribonuclease activity of bacterial, eukaryotic and ar

53 n is limited to 5 nucleotides, the intrinsic exoribonuclease activity of pol III cleaves 5-mer RNA at

54 vation of Rat1 by Rai1 and for the exclusive exoribonuclease activity of Rat1.

55 tion, purified Rai1p stabilized the in vitro exoribonuclease activity of Rat1p.

56 On the other hand, removal of the exoribonuclease activity of RNase BN in a cell lacking o

57 in the E. coli chromosome revealed that the exoribonuclease activity of RNase BN is not required for

58 The exoribonuclease activity of RNase BN is unnecessary beca

59 This impairs the 5'-3' exoribonuclease activity of RNase J1, increasing the hal

60 Loss of the exoribonuclease activity of RNase R was sufficient to in

61 cleavage become substrates for the 5' to 3'-exoribonuclease activity of the enzyme.

62 The exosome is named for the 3' to 5' exoribonuclease activity provided by a large C-terminal

63 Purified TREX1 displays robust exoribonuclease activity that degrades single-stranded,

64 have the largest RNA genomes, and encode an exoribonuclease activity that is required for high-fidel

65 The aptamers resisted DXO's exoribonuclease activity, and in studies monitoring DXO'

66 Consistent with the DEDD fold, Rv2179c has exoribonuclease activity, cleaving the 3' single-strand

67 at of Bacillus subtilis RNase Z, which lacks exoribonuclease activity, revealed that RNase BN has a n

68 In vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridin

69 vides the sole source of processive 3'-to-5' exoribonuclease activity.

70 ce that petD mRNA can be degraded by 5'-->3' exoribonuclease activity.

71 evaluated biochemically by monitoring nsp14 exoribonuclease activity.

72 non-catalytic core and Rrp44, which inhibit exoribonuclease activity; and features of the Rrp44 exor

73 show that a noncoding RNA interacts with an exoribonuclease, altering its substrate specificity and

74 nd nsp16, stimulating their respective 3'-5' exoribonuclease and 2'-O-methyltransferase activities.

75 one suppressor, DIS3/RRP44, encodes a 3'-5' exoribonuclease and a member of the multisubunit exosome

76 eviously shown to act as both a distributive exoribonuclease and an endoribonuclease on model RNA sub

77 polynucleotide phosphorylase (PNPase), a 3'-exoribonuclease and component of the RNA degradosome.

78 The exosome has both 3' to 5' exoribonuclease and endoribonuclease activity, and the a

79 It is an exoribonuclease and integral component of the multienzym

80 lly and functionally interacts with the Rrp6 exoribonuclease and its associated cofactor Rrp47, the h

81 Mammalian PNPase exhibits exoribonuclease and poly(A) polymerase activities, and P

82 Polynucleotide phosphorylase (PNPase) is an exoribonuclease and poly(A) polymerase postulated to fun

83 cleotide phosphorylase (PNPASE), a 3' --> 5' exoribonuclease and poly-A polymerase, in the mitochondr

84 PNPase is a 3'-to-5' exoribonuclease and promotes the processive degradation

85 h nsp14, a bifunctional enzyme bearing 3'-5' exoribonuclease and RNA cap N7-guanine methyltransferase

86 gher-order degradation complex consisting of exoribonucleases and a decapping activity, which togethe

87 subtilis is accomplished by a combination of exoribonucleases and endoribonucleases.

88 A stochastic competition between the exoribonucleases and poly(A) polymerase is proposed to e

89 nd on their catalytic properties, all of the exoribonucleases and their homologs have been grouped in

90 that concomitant loss of XRN4/EIN5, a 5'-3' exoribonuclease, and ABH1/CBP80, a subunit of the mRNA c

91 he terminal uridylyl transferase, U-specific exoribonuclease, and ligase activities of editing were u

92 , an endoribonuclease and processive 3'-->5' exoribonuclease, and Rrp6, a distributive 3'-->5' exorib

93 can be used to characterize newly-discovered exoribonucleases, and based on these motifs we correct s

94 rminus in conjunction with a generic 5'-->3' exoribonuclease; and (iii) remodels the structure of the

95 Exoribonucleases are vital in nearly all aspects of RNA

96 se(old-35)), an evolutionary conserved 3',5'-exoribonuclease, as a gene up-regulated during both term

97 tRNA, but not 5S RNA, any one of a number of exoribonucleases can carry out the trimming reaction in

98 contrast, in Escherichia coli, a variety of exoribonucleases carry out final 3' maturation.

99 The exosome is a 3' to 5' exoribonuclease central to many cellular processes, incl

100 Ai) screen and identified a putative 3'-->5' exoribonuclease CG9247/nibbler essential for the generat

101 osome is an essential and conserved 3'-to-5' exoribonuclease complex that degrades or processes nearl

102 The RNA exosome is a multisubunit 3' to 5' exoribonuclease complex that participates in degradation

103 iral strategy to interfere with the 5'-to-3'-exoribonuclease component of the cytoplasmic RNA decay m

104 mes from yeast and humans contain two active exoribonuclease components, Rrp6p and Dis3p/Rrp44p.

105 Exosome complexes are 3' to 5' exoribonucleases composed of subunits that are critical

106 We demonstrate that the 3' to 5' exoribonuclease decay pathway is a major contributor to

107 adducts in RNA directly by adduct-inhibited exoribonuclease degradation.

108 NPase(old-35)), a type I IFN-inducible 3'-5' exoribonuclease, degrades specific mRNAs and small nonco

109 unexpected source, pre-ribosomal RNA, in an exoribonuclease-dependent but DiGeorge syndrome critical

110 lyses strongly support a unified model of an exoribonuclease-dependent IFN suppression mechanism shar

111 An exoribonuclease-dependent scavenger decapping activity w

112 previously identified a complex of 3' --> 5' exoribonucleases, designated the exosome, that is expect

113 ination factors cooperate with the conserved exoribonuclease Dhp1/Rat1/Xrn2, which couples pre-mRNA 3

114 ature of mRNAs that provides protection from exoribonuclease digestion and enhances translation.

115 We use exoribonuclease digestion and targeted RNA-sequencing to

116 an endoribonucleolytic cleavage followed by exoribonuclease digestion to generate 5'-mononucleotides

117 y the exosome complex containing the nuclear exoribonuclease Dis3.

118 te in cells and virions when the cytoplasmic exoribonuclease DIS3L2 and subunits of the RNA exosome a

119 ide an atomic view of a catalytically active exoribonuclease domain of LASV NP (LASV NP-C) in the pro

120 gh-resolution crystal structure of an active exoribonuclease domain of Tacaribe arenavirus (TCRV) NP.

121 This RNase D 3'-->5' exoribonuclease domain was predicted to have a structure

122 hondrial RNA helicase that complexes with an exoribonuclease, DSS1, to function as an RNA degradosome

123 ity of their target protein, mouse decapping exoribonuclease (DXO).

124 Here, we show that a family of exoribonucleases encoded by the SMALL RNA DEGRADING NUCL

125 that protects the RNA from degradation by 5' exoribonucleases, ensures efficient expression of viral

126 anine N7-methyltransferase (MTase) and 3'-5' exoribonuclease (ExoN) activities.

127 y syndrome CoV (SARS-CoV) nsp14 has 3'-to-5' exoribonuclease (ExoN) activity in vitro.

128 The nsp14 protein carries both exoribonuclease (ExoN) and (guanine-N7)-methyltransferas

129 An L1 within ERI1 exoribonuclease family member 3 (ERI3) was found to asso

130 We have purified a 3'-5'-exoribonuclease from mitochondrial extract of Leishmania

131 Most exoribonucleases function with cofactors that recognize

132 in the rnc3/4 double mutant, suggesting that exoribonucleases generated staggered ends in the absence

133 Four 3'-to-5' exoribonucleases have been identified in Bacillus subtil

134 Loss-of-function mutations in 3'-to-5' exoribonucleases have been implicated in hereditary huma

135 Escherichia coli RNase R, a 3' --> 5' exoribonuclease homologous to RNase II, was overexpresse

136 tified dominant alleles of RAT1, encoding an exoribonuclease homologous with Xrn1p.

137 Exoribonuclease I from yeast is a 175 kDa protein that i

138 In this work, we report that the only exoribonuclease identified in M. genitalium, RNase R, is

139 ces similar to these endo- (HII and III) and exoribonucleases (II, PH and D).

140 RNase R, an Escherichia coli exoribonuclease important for degradation of structured

141 istence of another, as yet unknown, 3'-to-5' exoribonuclease in B. subtilis is suggested.

142 s results, show that the normal role of this exoribonuclease in imaginal discs is to suppress the exp

143 family members in that it also can act as an exoribonuclease in vitro.

144 ants of XRN1, encoding the major cytoplasmic exoribonuclease in yeast, are viable but accumulate deca

145 tholog Rsr (Ro sixty related) functions with exoribonucleases in 23S rRNA maturation.

146 n Rrp43p co-purifies with four other 3'-->5' exoribonucleases in a complex that has been termed the e

147 orylase (cpPNPase) are the two known 3'-->5' exoribonucleases in Arabidopsis chloroplasts, and are in

148 veal the cooperative activity of two 3'-->5' exoribonucleases in chloroplast mRNA 3' end maturation,

149 of RNase R and how it is distinct from other exoribonucleases in E. coli.

150 from the RNR family of processive, 3' to 5' exoribonucleases in Escherichia coli.

151 Here, we compare the roles of these two exoribonucleases in HCV-infected cells and confirm that

152 Here, we dissect the roles of these two 5' exoribonucleases in restricting the replication of diffe

153 However, RNase R differs from other exoribonucleases in that it can by itself degrade RNAs w

154 RNase R is unusual among exoribonucleases in that, by itself, it can digest throu

155 c region (i) blocks both 5'-->3' and 3'--> 5 exoribonucleases in vitro; (ii) is sufficient to define

156 amount of RNase R, an important degradative exoribonuclease, increases 3-10-fold under a variety of

157 RNase R, an important exoribonuclease involved in degradation of structured RN

158 The cytoplasmic exosome, a complex of 3'-5' exoribonucleases involved in RNA degradation and process

159 t of the exosome, the main cellular 3'-to-5' exoribonuclease, is a positive regulator of cuticular wa

160 cherichia coli RNase R, a processive 3'-to5'-exoribonuclease, is dramatically increased in response t

161 onstrate that RNase R, a processive 3'-to-5' exoribonuclease, is recruited to stalled ribosomes for t

162 rt that RNase R, a highly conserved 3' to 5' exoribonuclease, is required for the selective degradati

163 RNase II, a 3' to 5' processive exoribonuclease, is the major hydrolytic enzyme in Esche

164 horylase (PNPase), a 3'-to-5' phosphorolytic exoribonuclease, is thought to be the primary enzyme res

165 narily conserved complex of multiple 3'-->5' exoribonucleases, is responsible for a variety of RNA pr

166 We conclude that Xrn1 is the dominant 5' exoribonuclease mediating decay of HCV RNA and that miR-

167 OLD-35)), an evolutionarily conserved 3', 5' exoribonuclease mediating mRNA degradation, was first id

168 nated activities of ySuv3 helicase and yDss1 exoribonuclease (mtEXO), whereas in bacteria, RNA is deg

169 to the mitochondrion, a poly(A) specific 3' exoribonuclease, mtPARN, and a poly(A)binding protein, m

170 Decay of rpsO mRNA in a panel of 3'-to-5' exoribonuclease mutants was analyzed using a 5'-proximal

171 Genetically stable exoribonuclease mutants will allow direct testing of vir

172 RNAs undergo 3' end trimming by the 3'-to-5' exoribonuclease Nibbler (CG9247).

173 ein-protein interaction between the 3'-to-5' exoribonuclease Nibbler (Nbr) and Piwi that links Nbr ac

174 ucleoplasm trafficking, controlling 5'-to-3' exoribonuclease nucleolar levels and regulating rRNA pro

175 x that threads RNAs directly to the 3'-to-5' exoribonuclease of the cytoplasmic exosome, compensated

176 terestingly, other major 3'-to-5' processing exoribonucleases of E. coli, such as polynucleotide phos

177 catalytic properties compared with the other exoribonucleases of E. coli.

178 terestingly, RNase BN acts as a distributive exoribonuclease on some substrates, releasing mononucleo

179 s, 10 of which are predicted to be 3' --> 5' exoribonucleases on the basis of sequence homology.

180 ning precursors, RNase BN acted as either an exoribonuclease or endoribonuclease depending on the nat

181 Seq) assay, we identify the poly(A)-specific exoribonuclease PDE12 as a major factor for the quality

182 Exoribonucleases play an important role in all aspects o

183 RNase R, a ubiquitous 3' exoribonuclease, plays an important role in many aspects

184 omponents: the endoribonuclease RNase E, the exoribonuclease PNPase, the RNA helicase RhlB and enolas

185 Although the 3' to 5' processive exoribonucleases, PNPase and RNase II, have long been co

186 es: polynucleotide phosphorylase, hydrolytic exoribonuclease, poly(A) polymerase, and CCA transferase

187 n reading frames (ORFs) potentially encoding exoribonucleases, poly(A) polymerases, and proteins know

188 slowed because of the absence of processing exoribonucleases; poly(A) tails vary from one to seven r

189 rRNA profiles reveals that Rsr, the 3' to 5' exoribonuclease polynucleotide phosphorylase (PNP) and a

190 It has been demonstrated that the exoribonuclease polynucleotide phosphorylase (PNPase) fa

191 We report that Rsr and the exoribonuclease polynucleotide phosphorylase (PNPase) fo

192 Previous work has shown that the exoribonuclease polynucleotide phosphorylase (PNPase) is

193 Escherichia coli for tRNA(Leu5) in which the exoribonuclease polynucleotide phosphorylase (PNPase) re

194 We previously showed that the exoribonuclease polynucleotide phosphorylase (PNPase) wa

195 dosome are the endoribonuclease RNase E, the exoribonuclease polynucleotide phosphorylase (PNPase), a

196 t enzyme in organellar RNA metabolism is the exoribonuclease polynucleotide phosphorylase (PNPase), w

197 round in which expression of the chloroplast exoribonuclease polynucleotide phosphorylase was diminis

198 aintain cell viability in the absence of the exoribonuclease polynucleotide phosphorylase was markedl

199 enetic and biochemical interactions with the exoribonuclease polynucleotide phosphorylase, Rsr likely

200 nitase, a DEAD-box RNA helicase RhlB and the exoribonuclease polynucleotide phosphorylase.

201 sing three previously characterized 3'-to-5' exoribonucleases (polynucleotide phosphorylase [PNPase],

202 The activity of the phosphorolytic exoribonuclease, polynucleotide phosphorylase (PNPase),

203 ned the expression of pnp encoding the 3'-5'-exoribonuclease, polynucleotide phosphorylase, in Strept

204 a chloroplasts contain at least two 3' to 5' exoribonucleases, polynucleotide phosphorylase (PNPase)

205 in of Bacillus subtilis lacking two 3'-to-5' exoribonucleases, polynucleotide phosphorylase (PNPase)

206 RNase T is one of eight distinct 3'-->5' exoribonucleases present in Escherichia coli.

207 n vitro RNA degradation assays confirmed its exoribonuclease properties, and overexpression of hPNPas

208 st nuclear exosome contains multiple 3'-->5' exoribonucleases, raising the question of why so many ac

209 this cleavage is rapidly degraded via the 5' exoribonuclease Rat1p which is thought to destabilize th

210 of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover.

211 nucleotide phosphorylase (PNPase; a 3'-to-5' exoribonuclease) revealed a striking overexpression of t

212 ional degradosome component is the essential exoribonuclease RNase D, and its recognition site within

213 The subcellular localization of the exoribonuclease RNase II is not known despite the advanc

214 as homology to the Escherichia coli 3' to 5' exoribonuclease RNase PH, and both the Ski6p/Rrp41p and

215 ration is efficient and requires Rsr and the exoribonucleases RNase PH and RNase II.

216 In Escherichia coli, the exoribonucleases RNase R and polynucleotide phosphorylas

217 molecular mass of 90.5 kDa, and exhibited an exoribonuclease (RNase R) activity.

218 ein (NPs) of all arenaviruses carry a unique exoribonuclease (RNase) domain that has been shown to be

219 leoproteins (NPs) revealed a conserved DEDDH exoribonuclease (RNase) domain that is important for typ

220 droxyl acylation analyzed by protection from exoribonuclease (RNase-detected SHAPE) should prove broa

221 Interestingly, the third 3'-to-5' processing exoribonuclease, RNase R of E. coli, which is cold induc

222 tected SHAPE that uses a processive, 3'-->5' exoribonuclease, RNase R, to detect covalent adducts in

223 2'-O-adducts block processivity of a 3'-->5' exoribonuclease, RNase R, to produce fragments that term

224 an RNA chaperone, CspA, and a cold-inducible exoribonuclease, RNase R.

225 hia coli, has been found to encode the 3'-5' exoribonuclease, RNase R.

226 In contrast to other exoribonucleases, RNase R can efficiently degrade highly

227 Here, we show that four 3'-->5'-exoribonucleases, RNases II, R, and PH, and polynucleoti

228 nit exosome core, while Rrp47 stabilizes the exoribonuclease Rrp6 and recruits Mtr4, but it is less c

229 yces cerevisiae, the nuclear/nucleolar 3'-5' exoribonuclease Rrp6 distinguishes the nuclear exosome f

230 9 subunits that associate with the 3' to 5' exoribonucleases Rrp6, and Rrp44/Dis3, a subunit that al

231 We further identified a 3' to 5' exoribonuclease, RRP6 (ribosomal RNA processing protein

232 NA precursor and that defects in the nuclear exoribonuclease Rrp6p enhance this effect.

233 nuclease activity; and features of the Rrp44 exoribonuclease site that support its ability to degrade

234 Oligoribonuclease, a 3'-to-5' exoribonuclease specific for small oligoribonucleotides,

235 However, although other Escherichia coli exoribonucleases stop several nucleotides downstream of

236 bacteria possess additional hydrolytic 3'-5' exoribonucleases such as RNase II, RNase R was found to

237 es not stimulate the activity of other 5'-3' exoribonucleases, such as Rat1, in vitro.

238 perties of the distributive U-specific 3'-5'-exoribonuclease suggest an involvement in the U-deletion

239 The Trypanosoma brucei exoribonuclease, TbDSS-1, has been implicated in multipl

240 We have identified a mitochondrial exoribonuclease, TbRND, whose expression is highly up-re

241 yme was found to be a single-strand-specific exoribonuclease that acts in the 3' to 5' direction in a

242 In summary, TbRND is a novel 3' to 5' exoribonuclease that appears to have evolved a function

243 ) nucleoprotein (NP) is the only known 3'-5' exoribonuclease that can suppress type I interferon (IFN

244 the 5' direction, suggesting that LigD is an exoribonuclease that cleaves the 3'-terminal phosphodies

245 otide phosphorylase (PNPase) is a processive exoribonuclease that contributes to messenger RNA turnov

246 RNase R is a 3' to 5' hydrolytic exoribonuclease that has the unusual ability to digest h

247 teins identified oligoribonuclease (Orn), an exoribonuclease that hydrolyzes two- to five-nucleotide-

248 Nase R appears to be the only known 3' to 5' exoribonuclease that is able to degrade through double-s

249 ase(old-35)) is a type I IFN-inducible 3',5' exoribonuclease that mediates mRNA degradation.

250 Eri1 is an evolutionarily conserved 3'-5' exoribonuclease that participates in 5.8S rRNA 3' end pr

251 show that human USB1 is a distributive 3'-5' exoribonuclease that posttranscriptionally removes uridi

252 mice deficient in Eri1, a conserved 3'-to-5' exoribonuclease that represses RNA interference, have a

253 RNase R is a processive, 3' to 5' hydrolytic exoribonuclease that together with polynucleotide phosph

254 Surprisingly, Nibbler, a 3'-5' exoribonuclease that trims 'long' mature miRNAs in AGO1,

255 In general, RNA degradation is via exoribonucleases that degrade RNA either from the 5' end

256 fies with the exosome, a complex of 3' to 5' exoribonucleases that is implicated in the processing of

257 nd unneeded ncRNAs are primarily degraded by exoribonucleases that rely on protein cofactors to ident

258 RNA decapping enzymes hijack a host 5'-to-3'-exoribonuclease to evade antiviral innate immunity by li

259 a 275-nt RNA, which was then trimmed by a 3' exoribonuclease to the mature scRNA.

260 While exoribonuclease treatment is widely used to degrade line

261 and RNase PH, the only known P(i)-dependent exoribonucleases, were previously shown to grow slowly a

262 rs, including the mRNA-decapping complex and exoribonucleases, whereas another core factor, eIF4AIII/

263 acman/XRN1 is a highly conserved cytoplasmic exoribonuclease which degrades RNAs in a 5'-3' direction

264 f Saccharomyces cerevisiae encodes a 5'-->3' exoribonuclease which plays an essential role in yeast R

265 RNase R, was used to purify another 3'-to-5' exoribonuclease, which is encoded by the yhaM gene.

266 mber of the widely distributed RNR family of exoribonucleases, which are highly processive 3'-->5' hy

267 host genes, including XRN1, encoding a 5'-3' exoribonuclease, whose absence led to an approximately 1

268 RNase R is a processive 3'-5' exoribonuclease with a high degree of conservation in pr

269 racting protein partners, OIP2, is a 3'-->5' exoribonuclease with a phosphorolytic activity that proc

270 tion required Xrn1p but not Rat1p, a nuclear exoribonuclease with functional similarity to Xrn1p.

271 cases and 2,816 controls implicated PARN, an exoribonuclease with no previous connection to telomere

272 transiently protect nascent ncRNA ends from exoribonucleases, with partner proteins that sequester t

273 uses (CoVs) are unique in encoding a 3'-->5' exoribonuclease within nonstructural protein 14 (nsp14-E

274 esults further confirm that SOX and the host exoribonuclease Xrn1 act in concert to elicit the rapid

275 these products was dependent on the 5'-->3' exoribonuclease Xrn1 and not the exosome.

276 virus (VACV) decapping enzymes and cellular exoribonuclease Xrn1 catalyze successive steps in mRNA d

277 litate the activity of the cytoplasmic 5'-3' exoribonuclease Xrn1 in eukaryotes.

278 The conserved multidomain exoribonuclease Xrn1 targets cytoplasmic RNA substrates

279 Furthermore, YTHDC2 recruits the 5'-->3' exoribonuclease XRN1 via Ankyrin repeats that are insert

280 The cytoplasmic 5' to 3' exoribonuclease XRN1 was responsible for the degradation

281 to accomplish this is to target the cellular exoribonuclease XRN1, because this enzyme is accessible

282 st-transcriptional mechanisms, involving the exoribonuclease Xrn1, to compensate the derepression of

283 radation factor Pat1/Mtr1 and with the 5'-3' exoribonuclease Xrn1.

284 SA4 mRNA accumulated in cells lacking the 5' exoribonuclease Xrn1.

285 fRNA results from stalling of the host 5'-3' exoribonuclease XRN1/Pacman on conserved RNA structures

286 from 5' decay mediated by the cytoplasmic 5' exoribonuclease, Xrn1.

287 ainst decay mediated by distinct cellular 5' exoribonucleases, Xrn1 and Xrn2.

288 res the decapping enzyme Dcp1p, the 5'-to-3' exoribonuclease Xrn1p, and the three nonsense-mediated m

289 ages for degradation in the cytoplasm by the exoribonuclease Xrn1p.

290 The 5'-3' exoribonucleases Xrn1p and Xrn2p/Rat1p function in the d

291 ilized by the deletion of the cytoplasmic 5' exoribonuclease (Xrn1p) or by inactivation of the cytopl

292 XRN1 encodes an abundant cytoplasmic exoribonuclease, Xrn1p, responsible for mRNA turnover in

293 is homolog of the major yeast mRNA degrading exoribonuclease, Xrn1p.

294 by facilitating the interaction between the exoribonuclease XRN2 and select PDE transcripts.

295 diated by NKRF interaction with the 5'-to-3' exoribonuclease XRN2, a key coordinator of multiple pre-

296 horylated by Cdk9 was the 5'-to-3' "torpedo" exoribonuclease Xrn2, required in transcription terminat

297 We discovered that the 5'-3' exoribonuclease Xrn2, which plays a crucial role in the

298 port suggesting that a predominantly nuclear exoribonuclease, Xrn2, mediates the degradation of genot

299 tion of ETHYLENE-INSENSITIVE5 as the 5'-->3' exoribonuclease XRN4.

300 The 5'-->3' exoribonucleases (XRNs) comprise a large family of conse