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1 prominent loose-fitting structure called the exosporium.
2 o examine the distribution of Alr within the exosporium.
3 o material from the spore's core and not the exosporium.
4 prominent loose-fitting structure called the exosporium.
5 s required for the attachment of BclA to the exosporium.
6  in the outer spore coat and absent from the exosporium.
7 y a prominent loose fitting layer called the exosporium.
8 ther surrounded by a glycoprotein-containing exosporium.
9 loose-fitting, balloon-like layer called the exosporium.
10 n-like protein, BclB, is also present in the exosporium.
11 an important role in the construction of the exosporium.
12 hat is sufficient for incorporation into the exosporium.
13 nt for proper incorporation of BclA onto the exosporium.
14  NTD being sufficient to deliver BxpB to the exosporium.
15 lays a critical role in the formation of the exosporium.
16 roduce spores containing an outer layer, the exosporium.
17 hich is normally tightly associated with the exosporium.
18 l as a more interior location underneath the exosporium.
19 ar compatible with a protective role for the exosporium.
20 eveloping bacilli within the confines of the exosporium.
21 icated by previous proteomic analyses of the exosporium.
22                The spore is surrounded by an exosporium, a loose-fitting membrane composed of protein
23                                This unstable exosporium also lacked the enzyme alanine racemase, whic
24 rected toward BclA, a major component of the exosporium, although other components were also distingu
25  B. anthracis: it guides the assembly of the exosporium, an outer structure encasing B. anthracis but
26 cis DeltaSterne resulted in a partial second exosporium and in smaller spores.
27 n N-terminal sequence that targets it to the exosporium and is similar in sequence to a cognate targe
28 eins, Alr is nonuniformly distributed in the exosporium and might have a second spore location.
29 tures of bacterial endospores, including the exosporium and spore coats of four Bacillus species in a
30 bclB mutant spores, the distance between the exosporium and the coat, the interspace, is reduced.
31 macrophage-mediated killing of nonsonicated (exosporium+) and sonicated (exosporium-) Sterne 34F2 spo
32 or probing the structure and function of the exosporium, and in the analysis of the life cycle of B.
33 e assembly and maturation of ExsK within the exosporium are dependent on ExsFA/BxpB and BclA.
34 es by which they are assembled to create the exosporium are largely unknown.
35  The composition and assembly process of the exosporium are poorly understood.
36 ce that follows the site of formation of the exosporium around the forespore.
37  during sporulation and the only part of the exosporium assembled in a DeltaexsY mutant strain of B.
38 or cap, corresponds to the first part of the exosporium assembled within the mother cell during sporu
39 sults provide the first direct evidence that exosporium assembly is a non-uniform process and suggest
40                        Our results show that exosporium assembly on DeltaexsY spores is aberrant, wit
41 s altered, suggesting that BclB has roles in exosporium assembly.
42              The authors refined a model for exosporium assembly.
43 a disaccharide, are released from spore- and exosporium-associated BclA by hydrazinolysis.
44 ore than 20 proteins have been identified as exosporium-associated, their abundance, relationship to
45 indicating that ExsB was required for stable exosporium attachment.
46 of the protein at the site of the developing exosporium basal layer and stable incorporation which in
47 protein is a major structural protein of the exosporium basal layer of B. cereus family spores and th
48                      In spores that lack the exosporium basal layer protein ExsFA/BxpB, ExsK fails to
49 tibodies, we showed that BetA resides in the exosporium basal layer, likely underneath BclA.
50  revealed a fragile sac-like sublayer of the exosporium basal layer, to which caps were attached.
51 xpB (also called ExsF) is a component of the exosporium basal layer.
52 he structural features characteristic of the exosporium basal layer.
53 ed 2D arrays that mimic the structure of the exosporium basal layer.
54 mutant spores were encased by a double-layer exosporium, both layers of which were composed of a basa
55 red over approximately three-quarters of the exosporium but not in a cap-like region at one end of th
56 hich BclA and BetA are incorporated into the exosporium by a mechanism that depends on their similar
57 A and BclB are directed to assemble into the exosporium by motifs in their N-terminal domains.
58 s are included in stable high-molecular-mass exosporium complexes.
59 res of Bacillus anthracis are enclosed by an exosporium composed of a basal layer and an external hai
60 f anthrax, are enclosed by a loosely fitting exosporium composed of a basal layer and an external hai
61 res of Bacillus anthracis are enclosed by an exosporium composed of a basal layer and an external hai
62 Bacillus anthracis spores are enclosed by an exosporium comprised of a basal layer and an external ha
63  the Bacillus anthracis spore consists of an exosporium comprised of an outer hair-like nap layer and
64  the Bacillus anthracis spore consists of an exosporium comprised of two distinct layers, an outer ha
65 res, which cause anthrax, are enclosed by an exosporium consisting of a basal layer and an external h
66                                          The exosporium consists of a basal layer and an external hai
67 ded by the densely packed endospore coat and exosporium, containing amyloid or amyloid-like proteins.
68 eltabxpB spores, which lack BxpB, contain an exosporium devoid of hair-like nap even though the Delta
69     Their findings may have implications for exosporium formation in other spore forming bacteria, in
70 ly is a non-uniform process and suggest that exosporium formation is discontinuous.
71 e to the other, possibly indicating that the exosporium forms a semi-permeable barrier.
72                                           An exosporium forms the outermost layer of some spores; it
73 ene that (along with bclA) may be part of an exosporium genomic island.
74  appears to be a structural component of the exosporium hair-like nap.
75 at the chemokine was located internal to the exosporium in association primarily with the spore coat
76 nd assembly and suggest a novel role for the exosporium in germination.
77           BxpB and BclA are co-dependent for exosporium incorporation, with the BclA NTD being suffic
78                                          The exosporium is composed of a basal layer and an external
79                                          The exosporium is composed of a basal layer and an external
80 rulent in animal models, indicating that the exosporium is dispensable for infection, at least in the
81                                          The exosporium is the outermost layer of the Bacillus anthra
82                                          The exosporium is the site of interactions between the spore
83 , the interaction between the host and spore exosporium is vital to the initiation of disease.
84                      The outermost layer, or exosporium, is a bag-like structure that interacts with
85 ost structure of the B. anthracis spore, the exosporium, is a shell composed of approximately 20 prot
86 t layer of the Bacillus anthracis spore, the exosporium, is composed of a paracrystalline basal layer
87 fic location of this glycoprotein within the exosporium layer and its role in the biology of the spor
88                                The outermost exosporium layer of B. anthracis spores contains an exte
89 s found to possess structural defects in the exosporium layer of the spore (visualized by electron mi
90 the incorporation of these proteins into the exosporium layer of the spore and used these targeting d
91 ycoprotein, BclB, has been identified in the exosporium layer.
92 spore is not immunologically inert, (ii) the exosporium masks epitopes recognized by the Mphi, (iii)
93 on these data, we propose a revised model of exosporium maturation and assembly and suggest a novel r
94  the spore form of B. anthracis and that the exosporium may play a role in the protection of spores f
95 sporulation, germination, and spore coat and exosporium morphogenesis.
96 ng sporulation, as it enhances levels of the exosporium morphogenetic protein CdeC in a sigma(K) -dep
97  explained by differences in their shape and exosporium morphology, which may result in differences i
98 l location of structural proteins within the exosporium, namely a description of its three-dimensiona
99                             In contrast, the exosporium of B. anthracis, which exhibits arginase acti
100                                          The exosporium of Bacillus anthracis is comprised of two dis
101                                          The exosporium of Bacillus anthracis spores consists of a ba
102 A) is the immunodominant glycoprotein on the exosporium of Bacillus anthracis spores.
103 n immunodominant glycoprotein located on the exosporium of Bacillus anthracis.
104 characterized crystalline fragments from the exosporium of Bacillus cereus, B. thuringiensis and B. a
105 ted to incorporate foreign proteins into the exosporium of inactivated, spores resulting in the surfa
106                                          The exosporium of the Bacillus cereus group, including the a
107  racemase presumed to be associated with the exosporium of wild-type spores.
108 id of ExsY, and examined the assembly of the exosporium on spores produced by this strain.
109        betA is present in several genomes of exosporium-producing Bacillus species but, so far, not i
110 s essential for the recognition of the major exosporium protein BclA by phagocytic cells.
111 ative agent of anthrax, whose immunodominant exosporium protein BclA contains collagen-like repeat se
112 he previously identified but uncharacterized exosporium protein ExsK.
113 ing a visible exosporium were devoid of most exosporium proteins but, surprisingly, retained the puta
114 ins but, surprisingly, retained the putative exosporium proteins BxpC and CotB-1.
115 t in bclB mutant spores, the distribution of exosporium proteins CotY and BxpB is altered, suggesting
116 ic form in close association with many other exosporium proteins in high-molecular weight complexes.
117 that BetA is a member of a growing family of exosporium proteins that assemble under the control of t
118                   Unlike other characterized exosporium proteins, Alr is nonuniformly distributed in
119          We found that, in contrast to other exosporium proteins, ExsK is present in at least two dis
120 e proteins, 6 of which are candidate coat or exosporium proteins.
121 oclonal antibodies, raised against spores or exosporium, reacted with the CTD, consistent with its ex
122                          The function of the exosporium remains poorly understood and is an area of a
123 hment of essentially all BclA trimers to the exosporium requires the basal layer protein BxpB, and bo
124                                 Although the exosporium serves as the source of surface antigens and
125  the outgrowing cell always escapes from its exosporium shell by popping through the cap, suggesting
126 ation of purified DeltaexsY spores devoid of exosporium showed that they lacked detectable levels of
127                                          The exosporium spore layer was retained (exo+) or removed by
128 than wild-type spores, which had an extended exosporium (spore length for the wt, 2.40 +/- 0.56 micro
129 of nonsonicated (exosporium+) and sonicated (exosporium-) Sterne 34F2 spores.
130                  To date, exact roles of the exosporium structure and BclA protein remain undetermine
131 role in the formation and maintenance of the exosporium structure in B. anthracis.
132 er proteins that have been identified in the exosporium such as GroEL, immune inhibitor A and arginas
133 other molecular components anchored onto the exosporium, such as BclA and ExsFA.
134               The predominant protein on the exosporium surface is BclA, a collagen-like glycoprotein
135                        In spores lacking the exosporium surface protein BclA, ExsK fails to mature in
136 is motif, which we have named betA (Bacillus exosporium-targeted protein of B. anthracis).
137 lB is located principally in a region of the exosporium that excludes a short arc on one side of the
138 s partly due to the extreme stability of the exosporium that has proven to be refractive to existing
139 ess a loosely fitting outer layer called the exosporium that is composed of a basal layer and an exte
140 escence, and flow cytometry) resulting in an exosporium that is more fragile than that of a wild-type
141 le composition of the endospore coat and the exosporium that makes staining methodologies for endospo
142 stalline flexible yet resistant layer called exosporium that plays a major role in spore adhesion and
143 e to synthesize ExsB produced spores with an exosporium that was readily sloughed, indicating that Ex
144 that excludes a short arc on one side of the exosporium (the so-called bottle-cap region).
145 rine Bacillus spores oxidize Mn(II) on their exosporium, the outermost layer of the spore, encrusting
146  of anthracis) is a major constituent of the exosporium, the outermost surface of B. anthracis spores
147 le in directing the assembly of the coat and exosporium to an area around the forespore.
148                To examine the ability of the exosporium to protect spores from macrophages, we compar
149  purified DeltaexsB spores lacking a visible exosporium were devoid of most exosporium proteins but,
150 layer of the Bacillus anthracis spore is the exosporium, which is composed of a paracrystalline basal
151  and the morphogenesis of the spore coat and exosporium will be discussed.

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