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1 VP2 was provided in trans from a eukaryotic expression plasmid.
2 488 after infection or transfection of a US3 expression plasmid.
3 was restored by cotransfection with a ChREBP expression plasmid.
4 rom Treponema pallidum were cloned into this expression plasmid.
5 s expressing Jak2 via the addition of a Jak2 expression plasmid.
6 ansfected with a wild-type androgen receptor expression plasmid.
7 ls transiently transfected with a human TLR5 expression plasmid.
8 se blue (S) opsin gene was cloned into a Cre-expression plasmid.
9 ATP-binding cassette transporter A1 (ABCA1) expression plasmid.
10 only in cells cotransfected with a human VDR expression plasmid.
11 rescent protein genes, and (iii) an envelope expression plasmid.
12 ugmented by cotransfection with Sp1 or EGR-1 expression plasmid.
13 inase I cDNA or a beta-galactosidase control expression plasmid.
14 dominant negative mutant vector and a c-jun expression plasmid.
15 in-producing renal As4.1 cells with LXRalpha expression plasmid.
16 pression plasmid, as well as to a C/EBPalpha expression plasmid.
17 ion alone or in combination with thioredoxin expression plasmid.
18 was observed upon injection of 10 mug of the expression plasmid.
19 -infected Rael cells transfected with a LANA expression plasmid.
20 ms: a replication deficient adenovirus or an expression plasmid.
21 d 5-10-fold when cotransfected with a GATA-6 expression plasmid.
22 were enhanced by a co-transfected C/EBPdelta expression plasmid.
23 ng chain polylysine and a beta-galactosidase expression plasmid.
24 ored by the transient transfection of a CD81 expression plasmid.
25 in MDA-MB-231 cells transfected with ZBTB10 expression plasmid.
26 ably transfected with an inducible protein A expression plasmid.
27 Amplified products were cloned into an expression plasmid.
28 ently constructed the DENV-1, -3, and -4 VLP expression plasmids.
29 lease of virus-like particles encoded by Gag expression plasmids.
30 B receptor mRNAs were designed and cloned in expression plasmids.
31 lowing stable transfection with TLR2 or TLR5 expression plasmids.
32 ecreted Frizzled receptor protein 1 (sFRP-1) expression plasmids.
33 ic Cas9 lines and versatile guide RNA (gRNA) expression plasmids.
34 gical response of HCV transgenic mice to HCV expression plasmids.
35 oter activity induced by IRF-1 and NF-kappaB expression plasmids.
36 anisms, we generated a series of bicistronic expression plasmids.
37 fibroblast-like Cos cells+/-HNF3beta or GATA expression plasmids.
38 d into uninfected cells by transfection with expression plasmids.
39 were introduced into the cell via multicopy expression plasmids.
40 pa 1c1c7 cells using antisense Arnt and Arnt expression plasmids.
41 ontrol expression plasmid and/or with mutant expression plasmids.
42 tinoic acid receptor and retinoid X receptor expression plasmids.
43 gradation-resistant HIF-1alpha or HIF-2alpha expression plasmids.
44 viruses together with the supporting protein expression plasmids.
45 0-fold compared to conditional selection for expression plasmids.
46 gene (clyA::t-csp) in genetically stabilized expression plasmids.
47 ds within the cross-reactive epitopes of VLP expression plasmids.
48 ter plasmid in combination with NOD1 or NOD2 expression plasmids; 2) by inhibiting DMXAA-induced chem
49 n C2C12 myoblasts carrying an inducible p204 expression plasmid) accelerated the fusion of myoblasts
51 e co-transfection of HeLa cells with a B-Myb expression plasmid activated the transfected SP-A promot
52 studies used prime/boost combinations of DNA expression plasmids alone or DNA priming and boosting wi
53 the current work we electroporated an I-SceI expression plasmid along with HaeIII fragments of φX
54 iver regeneration after injection of an LSP1 expression plasmid also led to decreased hepatocyte prol
57 One fragment was subcloned into a bacterial expression plasmid and a GST-fusion protein was produced
59 ne, beginning with the design of a pro-siRNA expression plasmid and ending with siRNA purification.
63 d in normal FLS by transfection with a Wnt-1 expression plasmid and was down-regulated in RA FLS by t
64 which are transfected with either a control expression plasmid and/or with mutant expression plasmid
66 nes, luciferase derivatives were made of the expression plasmids and introduced into Aedes albopictus
68 r single plasmids or cocktails of up to four expression plasmids and then challenged with sublethal d
69 quences for the VEGF-A gene were cloned into expression plasmids and transfected into HT1080 human fi
70 re permanently transfected with a luciferase expression plasmid, and C6 experimental brain tumors wer
71 Ang(-S)Exp/pSVL (or a corresponding control) expression plasmid, and mitotic indices were measured fo
72 f the DNA capture events involved the I-SceI expression plasmid, and several events involved retrotra
73 incorporated via mutagenesis into the fiber expression plasmid, and the resulting fiber proteins wer
74 omoter was about 50% in both orientations in expression plasmid, and two transcriptional factors were
75 e or by co-transfecting with Runx2 antisense expression plasmid, and was enhanced by overexpression o
76 th the NF-kappa B-Luc reporter, CD14 and MD2 expression plasmids, and expression plasmids for TLR2, T
77 of endogenous DeltaNp63alpha, DeltaNp63-EGFP expression plasmids, and p53 were assessed after treatme
78 enotype 1b genes were cloned into eukaryotic expression plasmids, and the immunogenicity was determin
80 ine phosphatase 1B (PTP1B), and PTP1B mutant expression plasmids as well as SirT1 small interfering R
81 activity in response to a cotransfected Sp1 expression plasmid, as well as to a C/EBPalpha expressio
83 caques were coimmunized intramuscularly with expression plasmids bearing genes encoding Th1 (interleu
85 also stimulated by transfection with a STAT6 expression plasmid, but was inhibited by antisense oligo
86 SW480 colon cancer cells transfected with an expression plasmid (c-srcY527F) encoding a constitutivel
87 ltering the ratio of proviral genome and env expression plasmids can produce pseudovirions that are s
88 or ICAM-CAT, cotransfection with a CMV-IE72 expression plasmid caused promoter and CAT activation.
90 formation of the iscS(-) strain with an iscS expression plasmid complemented all of the observed phen
91 Ntcp promoter, while a dominant negative JNK expression plasmid completely blocked IL-1 beta-mediated
93 s, exchange is achieved by introducing a Cre-expression plasmid containing an equivalent cassette con
94 melanomas and HCCs were transfected with an expression plasmid containing argininosuccinate syntheta
96 rol cells, HEK293T cells transfected with an expression plasmid containing the c.5098C>T (p.Pro1700Se
98 t murine IL-6 or intradermal injection of an expression plasmid containing the full-length murine IL-
99 verexpression of GSH by transfection with an expression plasmid containing the GCLC cDNA conferred se
103 strains require a GlcNAc supplement and that expression plasmids containing both exogenous components
104 sfection of Chinese hamster ovary cells with expression plasmids containing different V(H) and V(L) s
106 isubunit DNA binding protein (EcoR124I) with expression plasmids containing the EcoR124I recognition
108 analysis of three in vivo-inducible promoter expression plasmids, containing pnirB, ppagC, and pkatG,
109 econstitution of ClC-3 expression with ClC-3 expression plasmids could rescue the cells from the cell
110 140,000 second-generation short hairpin RNA expression plasmids, covering a substantial fraction of
114 ansfection with the kinase-defective pp90RSK expression plasmid demonstrated a statistically signific
115 ous c-met promoter reporter vectors with p53 expression plasmids demonstrated that only wild-type p53
116 ocedure including construction of redesigned expression plasmids, development of automated protein ex
117 e reporter construct plus dHAND or dHAND-E12 expression plasmids did not alter luciferase activity, w
118 Furthermore, boosting with recombinant NS3 expression plasmid DNA after priming with M-ISA720/CpG-a
119 se two derivatives were cloned separately on expression plasmids downstream from the CFA/I operon.
120 me system in which T7 RNA polymerase from an expression plasmid drove expression of RNA transcripts f
121 m cells transfected with Smad1 linker region expression plasmid effectively inhibited osteoclastogene
122 ntrolateral o blast cells by injection of an expression plasmid elicited the dorsolateral P cell fate
123 Dividing the insert between three high-copy expression plasmids enables the bulk purification of the
125 perties, we favored the use of a bicistronic expression plasmid encoding both GFP and a beta subunit.
126 invasive i.v. administration of a 6- to 7-kb expression plasmid encoding either luciferase or beta-ga
127 onal and structural study of this enzyme, an expression plasmid encoding His-tagged human PMK has bee
129 cancer cells transiently transfected with an expression plasmid encoding pd1EGFP and treated with FZ
130 HepG2) were transiently transfected with the expression plasmid encoding PPARalpha and a plasmid cont
133 ion-deficient adenovirus, poly-L-lysine, and expression plasmids encoding beta subunits could be opti
134 ansfection of a GAL reporter gene along with expression plasmids encoding c-Jun plus c-Fos, or the ca
137 SRD-15 cells when they are transfected with expression plasmids encoding either Insig-1 or Insig-2.
138 tored when SRD-14 cells are transfected with expression plasmids encoding either Insig-1 or Insig-2.
140 ce system for the stabilization of multicopy expression plasmids encoding foreign antigens in a Salmo
142 NVAX(R)-B DNA vaccine (PV) is a mixture of 3 expression plasmids encoding HIV-1 Clade B Env, Gag, and
145 , model cells (HeLa) were cotransfected with expression plasmids encoding its mutant or wild-type (wt
146 uced by gene gun vaccination with eukaryotic expression plasmids encoding melanocyte differentiation
149 generated by cotransfecting 293T cells with expression plasmids encoding patient-derived Vif, human
150 ccidiosis both alone and in combination with expression plasmids encoding the interleukin 1 (IL-1), I
151 Transfection of this plasmid, along with the expression plasmids encoding the N, P, M2-1, and L prote
155 ells transiently transfected with a Rex cDNA expression plasmid failed to become persistently infecte
156 regimen with a Zaire ebolavirus glycoprotein expression plasmid followed by infection with a vesicula
158 ncode a transforming gene by transfecting an expression plasmid for JSRV [pCMVJS21, driven by the cyt
159 using particle-mediated gene transfer of an expression plasmid for postsynaptic density 95-green flu
160 PRB levels were elevated by transfecting an expression plasmid for PRB, but not when the cells were
161 ctor (eIF) 4H was subcloned into a bacterial expression plasmid for purification of recombinant prote
162 nsfected transiently with an OriP-containing expression plasmid for the positive prey together with e
163 k, an engineered E. coli strain harboring an expression plasmid for the Umbellularia californica acyl
165 broad range of t-Bu-DEX concentrations when expression plasmids for both receptors were cotransfecte
166 lls transfected with various combinations of expression plasmids for BRCA1, BRCA1 specific inhibitory
167 In addition, cotransfection of VSMC with expression plasmids for c-Fos and members of the Jun fam
168 e pairs of 5'-flanking sequence of GSTA2 and expression plasmids for either GR, pregnane X receptor (
169 deoxycholic acid (CDCA) and transfected with expression plasmids for farnesoid X-receptor (FXR), shor
170 mbinant L. monocytogenes carrying eukaryotic expression plasmids for feline tumor necrosis factor (TN
171 ction studies using the FR-beta promoter and expression plasmids for the nuclear receptors retinoic a
173 Cotransfection experiments with CREB and PKA expression plasmids further supported our conclusions th
174 n YB-1 binding and co-transfection of a YB-1 expression plasmid had a repressive effect on UV-inducib
175 s overcome by increasing amounts of a K-bZIP expression plasmid in the cotransfection mixture or by d
176 nly when additional ORF50 was supplied as an expression plasmid in the transfection mixture, suggesti
177 ected with increasing amounts of selected TF expression plasmids in addition to LCR-luciferase vector
179 H pylori strains, or transfected with a CagA expression plasmid, in the absence or presence of SMO sm
180 eficient cell line, MDA-MB-468, with an NQO1 expression plasmid increased apoptotic responses and let
181 e stimulated by cotransfection with an Hsp72 expression plasmid, indicating that hsp72 likely plays a
184 the present studies we transfected a Smurf1 expression plasmid into 2T3 osteoblast precursor cells a
185 We show first that transfection of an LMP1 expression plasmid into Ad-AH cells, an EBV-negative nas
186 pression as transient transfection of an AhR expression plasmid into AhR(-/-) fibroblasts significant
188 In contrast, transient transfection of MeCP2 expression plasmid into fibroblasts enhanced alpha-SMA g
190 e HBV replicative plasmid with RIG-I or MDA5 expression plasmid into Huh7 cells and found that MDA5,
191 -17, because transient transfection of TRAF6 expression plasmid into the TRAF6-deficient cells restor
193 ty-two hours after the transfection of eight expression plasmids into cocultured 293T and MDCK cells,
194 kappa opioid receptors, by transfecting dual-expression plasmids into COS-7 cells, the full-length GA
197 f drug resistance genes for the selection of expression plasmids introduced into live vectors poses t
201 by multiple site mutagenesis on recombinant expression plasmids is sufficient to convert the kinetic
202 d the mutant protein was synthesized from an expression plasmid, it concentrated in punctate cytoplas
203 injection into mice of IL-15 and IL-15Ralpha expression plasmids led to significantly increased level
204 When Sp1 levels were increased through an expression plasmid, luciferase reporter gene expression
205 10 harbored a specific segment of the I-SceI expression plasmid mapping between two replication origi
206 site (E-box), and cotransfection of a c-myc expression plasmid markedly enhanced the promoter activi
207 Three injections of a recombinant eukaryotic expression plasmid of BDV p10 were needed to generate a
209 he introduction into bacteria carrying a Red expression plasmid of synthetic (PCR-generated) DNA.
210 accomplished this through incorporation into expression plasmids of a postsegregational killing syste
211 at were transiently transfected with various expression plasmids of CK2 (thereby expressing additiona
214 by recoded allotopic ATP8 (nATP8) in a high-expression plasmid or in a CEN plasmid in the presence o
215 of US3 kinase in cells transformed with US3 expression plasmids or infected with each virus results
216 entation was provided by cotransfection with expression plasmids or infection with MV helper virus.
217 , using transfected fusion and hemagglutinin expression plasmids or with syncytium-based assays in Ve
218 expression of dominant-negative mutant TAp73 expression plasmid (p73DD) counteracted the MLN8054-indu
219 were spiked with different dilutions of the expression plasmid pA-EGFP_B, a PCN from 1 to 64 could b
223 When SK-BR-3 cells were transfected with the expression plasmid pCI-ER alpha, but not pCI-ER beta, ar
225 deliver mucosally to cotton rats eukaryotic expression plasmid pGA3-mH and Sindbis virus-based DNA r
228 In this study four murine IL-12 naked DNA expression plasmids (pIL-12), containing both the p35 an
229 cells cotransfected with PKA-Calpha and SOX9 expression plasmids, PKA enhanced the phosphorylation of
230 h injection of a dominant negative (DN) FoxO expression plasmid prior to inoculation with Lewis lung
231 anulocyte colony-stimulating factor (hG-CSF) expression plasmids produced high-level gene expression
232 and in vivo and that transfection of a DLX5 expression plasmid promotes the expression of MYC in a d
234 letion of env sequences from a JSRV proviral expression plasmid (pTN3) abolished its ability to produ
235 S medical countermeasures, we constructed an expression plasmid, pWRG/AND-M, that contains the full-l
236 sfection of these cells with a gamma-catenin expression plasmid reduced the elevated TCF activity by
237 endrimer nanoparticles containing a TNFalpha expression plasmid regulated by telomerase gene promoter
239 expression using a short hairpin RNA (shRNA) expression plasmid resulted in markedly reduced prolifer
240 n-mediated intramuscular injection of TIMP-4 expression plasmid resulted in sustained plasma TIMP-4 l
241 asmid simultaneously with C/EBPalpha and Sp1 expression plasmids resulted in an increase in luciferas
242 sfection of the MUC5AC-luc reporter with Sp1 expression plasmids resulted in significantly increased
244 3T cells transfected with TRbeta or LXRalpha expression plasmids show that TR, together with its liga
245 onstructs with a dominant-negative PKC-delta expression plasmid showed that suppression of this kinas
246 cert with PPARgamma, PPARalpha, or PPARdelta expression plasmids, showed dose-dependent activation of
247 ave demonstrated that transfection of a p300 expression plasmid significantly activated ferritin H-CA
248 onal level, transfection of SMCs with a Prx1 expression plasmid significantly increased their growth.
249 of autoantigen cDNAs to be incorporated into expression plasmids so as to generate tolerizing vaccine
250 Cotransfection with LXRalpha and RXRalpha expression plasmids strongly stimulated rat CYP7A1/lucif
252 amster ovary (CHO) cells transfected with an expression plasmid that contained cDNA coding for the ch
253 antly inhibited the expression of a reporter expression plasmid that contains the 3'-untranslated reg
254 pneumophila effectors was screened using an expression plasmid that produces low levels of each prot
255 was compared with that from a control, null-expression plasmid that was identical except for a frame
256 emA gene from C. vibrioforme was cloned into expression plasmids that added an N-terminal His(6) tag
257 able transfection of HEK293S-TetR cells with expression plasmids that contained the opsin gene downst
258 ed anti-RT antibodies and constructed HTLV-1 expression plasmids that express truncated or hemaggluti
260 l mice, were transfected with two mutant E1A expression plasmids that inactivate either p300/CBP or r
261 introduced into E. coli and induced from an expression plasmid, the mutant subunits act as a decoy i
262 is protocol (from the receipt of the QconCAT expression plasmid to the absolute quantification of the
263 t plasmid was transactivated by a C/EBPalpha expression plasmid to the same extent as wild-type LF-89
265 ruitment of pUL53 to the nuclear envelope in expression plasmid-transfected and human cytomegalovirus
266 Both proteins were encoded in two different expression plasmids under the control of different tight
267 We constructed the recombinant prokaryotic expression plasmid using prokaryotic expression vector (
270 fection of NLDeltaVpr proviral DNA and a Vpr expression plasmid was employed to analyze the virion in
271 o2 cells stably transfected with CARD15/NOD2 expression plasmid was lower than untransfected Caco2 ce
272 10T1/2 cells, but cotransfection with a SOX9 expression plasmid was sufficient to activate the enhanc
275 a MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a s
277 f viral structural proteins from the protein expression plasmids was not required for virus rescue, w
280 lated, a trcR-lacZ fusion plasmid and a TrcR expression plasmid were cotransformed into Escherichia c
281 Mammalian cells transfected with CeABF-1 expression plasmids were capable of blocking E2A-mediate
286 ndividual DeltaNp63 isoforms, DeltaNp63-EGFP expression plasmids were transiently expressed in hTCEpi
287 e of a SapI substrate site designed into the expression plasmid, which allows for in vitro selection
288 occurring plasmid partition function in our expression plasmids, which eliminates random segregation
289 ssion, we engineered lentivirus-driven miRNA expression plasmids, which we tested in the mouse placen
290 tudy, we show that cotransfection of the SEF expression plasmid with an SAA3/luciferase reporter resu
295 olated from cox10 deletion strains harboring expression plasmids with coxI and coxII or with coxI and
296 nt gp120 sequences were subcloned into gp160 expression plasmids with identical cleavage motifs and g
298 Interestingly, in most cell lines, JSRV expression plasmids with Rej deleted showed normal trans
300 transfection of ES cells with a pCS2/noggin expression plasmid, with differentiation peaking at 72 h
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