ライフサイエンスコーパス: expression system

1 to express functional PDE6 in a heterologous expression system.

2 ant proteins of A3F from an Escherichia coli expression system.

3 te odour-induced responses in a heterologous expression system.

4 ntly as a chimeric protein in a heterologous expression system.

5 genes in a Daucus carota (carrot) protoplast expression system.

6 e, recombinant enzyme in the baculovirus/Sf9 expression system.

7 nt, in fly tissues using the UAS-Gal4 binary expression system.

8 s and cell display platforms in a eukaryotic expression system.

9 patients were evaluated using a prokaryotic expression system.

10 receptors to human odorants with the Xenopus expression system.

11 y regulates KCNQ2/3 channels in a simplified expression system.

12 type I, were produced using a tobacco-based expression system.

13 flux using an in vitro cellular heterologous expression system.

14 y enhances its surface expression in HEK293T expression system.

15 tein function was assessed in a heterologous expression system.

16 in the malaria mosquito, AgGr25, in the ab1C expression system.

17 is of marineosins, using a marG heterologous expression system.

18 derived DCs (MDDCs) using a lentivirus-based expression system.

19 ased Bombyx mori nucleopolyhedrovirus bacmid expression system.

20 pha-onocerin in the N. benthamiana transient expression system.

21 cDNA and cloned it into an Escherichia coli expression system.

22 graphic purity was produced by a prokaryotic expression system.

23 as not secreted from S2 cells using the same expression system.

24 -C expressed using a baculovirus/insect cell expression system.

25 human, HEK293 cells, and in a Xenopus oocyte expression system.

26 2 were expressed in the wheat germ cell-free expression system.

27 were purified from the baculovirus/Sf9 cell expression system.

28 b separately, we employed the Xenopus laevis expression system.

29 for other allergens by using the pCANTAB 5 E expression system.

30 ient dual-luciferase((R)) reporter (Promega) expression system.

31 nsporter" (PfCRT) in a Xenopus laevis oocyte expression system.

32 teins with 8His tags using a T. thermophilus expression system.

33 us myosin-XXI constructs using a baculovirus expression system.

34 y transfer (FRET) analysis in a heterologous expression system.

35 ce expression of NKCC2, independently of the expression system.

36 were investigated in a Xenopus laevis oocyte expression system.

37 ila KCNQ potassium channel in a heterologous expression system.

38 nts, using a dual RNA polymerase II promoter expression system.

39 eption) were not affected in the HEK293 cell expression system.

40 adapted to other organisms with an inducible expression system.

41 Both vaccines were easily made via E. coli expression system.

42 ancement over and above the most widely used expression systems.

43 proteins in functional forms in heterologous expression systems.

44 vaccine testing in a variety of heterologous expression systems.

45 n various signaling pathways in heterologous expression systems.

46 logy toolkit beyond traditional constitutive expression systems.

47 well studied of all proteins in heterologous expression systems.

48 iants produced in prokaryotic and eukaryotic expression systems.

49 man ventricular myocytes and in heterologous expression systems.

50 expression of the receptors in heterologous expression systems.

51 s to fully understand even the simplest gene expression systems.

52 pled enzyme assays using microbial and plant expression systems.

53 produced in either prokaryotic or eukaryotic expression systems.

54 ectable TWIK-1 K(+) currents in heterologous expression systems.

55 ls in order to compare widely used mammalian expression systems.

56 localization of human ClC-4 in heterologous expression systems.

57 A11-tsC genetic context, we employed ectopic expression systems.

58 subtypes have been described in heterologous expression systems.

59 acted and purified from heterologous protein expression systems.

60 solely on protein derived from heterologous expression systems.

61 sing both constitutive and ethanol-inducible expression systems.

62 untered in the Golgi Apparatus in human cell expression systems.

63 w in both wide-type strains and heterologous expression systems.

64 ane proteins from prokaryotic and eukaryotic expression systems.

65 hat are rich in tyrosine codons in bacterial expression systems.

66 sly present in COS-7 cells employed as hGAT1 expression system, ((2)H6)GABA was used as a substrate t

67 ichia coli protein solubility in a cell-free expression system, 35 sequence-based properties are calc

68 To expand the versatility of the 2A expression system, a number of commonly used selectable

69 report a Caenorhabditis elegans heterologous expression system able to produce milligram amounts of f

70 e changes were studied using a new cell-free expression system and a mass spectrometry-based assay fo

71 rm of IL-11 in an optimized Escherichia coli expression system and administered it as the aerosol in

72 optimized pyrrolysyl-tRNA synthetase/tRNACUA expression system and an engineered eRF1, we increase th

73 rifying the mutants from an Escherichia coli expression system and assembling in vitro yielded NC ass

74 reen for ROMK function, we developed a yeast expression system and discovered that yeast cells lackin

75 be coimmunoprecipitated with CXCR4 in a HeLa expression system and in human VSMC.

76 Each droplet contains a cell-free expression system and is connected to its neighbor throu

77 ncoded cyanine dye as a ncAA in a eukaryotic expression system and opens the door for the analysis of

78 sed the NL219 hemagglutinin in a baculovirus expression system and performed a structural analysis of

79 Using a cell-free expression system and pulse-proteolysis experiments, we

80 xpressed ATGYRA and ATGYRB2 in a baculovirus expression system and shown supercoiling activity of the

81 c antibodies to be produced in virtually any expression system and still maintain effector function.

82 torsinADeltaE, we developed a yeast torsinA expression system and tested the roles of ER chaperones

83 uted PRL-induced signaling in a heterologous expression system and TG neurons from PRL receptor (PRLR

84 rda (Sf9) cells using a baculovirus-mediated expression system and the enzymatic activity and metabol

85 Using the Xenopus oocytes expression system and two microelectrode voltage-clamp,

86 of each receptor from recombinant bacterial expression systems and characterize their interactions w

87 In heterologous expression systems and cultured sensory neurons, exposur

88 s studies of DAT trafficking in heterologous expression systems and dissociated cultured neurons, stu

89 The results are discussed in the context of expression systems and downstream processing, contributi

90 synthetic utilization pathways as titratable expression systems and for metabolic engineering.

91 spoIIID and sigK mutants using heterologous expression systems and RNA-Seq transcriptional profiling

92 ced enhancer tools combined with conditional expression systems and the PiggyBac-mediated DNA transpo

93 and proteins in the creation of plant-based expression systems and virus particles for use in nanote

94 siveness in both heterologous and endogenous expression systems and whether this affects PTH secretio

95 of Kv2.1 mimicking apoptosis in a mammalian expression system, and protected cortical neurons from s

96 y expressed all 36 PvTRAgs using a cell-free expression system, and, for the first time, profiled the

97 evisiae (S. cerevisiae) Gpi8 using different expression systems, and examined its interaction with in

98 recombinant EDIII proteins and the different expression systems, and reached between 0.8 and 1.6 % of

99 ed against a number of commonly used E. coli expression systems, and shows tight basal control, preci

100 leading to significant improvements in these expression systems, and to deeper insights into the cell

101 Using an HEK293 cell expression system, antimycin A induced concentration-dep

102 Csy4 and tRNA expression systems are almost twice as effective in indu

103 plastid DNA (ptDNA) and its associated gene expression system, as reported recently in several linea

104 esent iTango, a light- and ligand-gated gene expression system based on a light-inducible split tobac

105 Here, we develop a compact shRNA (cshRNA) expression system based on retroviral microRNA (miRNA) g

106 Herein the distinct expression systems based on viral elements are analyzed.

107 ccine platform based on the baculovirus dual-expression system (BDES) was evaluated.

108 In heterologous expression systems, both the TMD and CR of PCDH15 have b

109 arrestin binding in an in vitro heterologous expression system but that also impairs receptor express

110 ractions in many cell types and heterologous expression systems, but interactions between alpha6beta4

111 shown to interact with AMPARs in artificial expression systems, but it is unknown whether Shisa7 has

112 Here we report a new transgenic expression system by combination of liver-specific expre

113 P1 was analyzed in the Xenopus laevis oocyte expression system by two-electrode voltage clamp and opt

114 ercomes the limitations of current inducible expression systems by enabling robust spatial and tempor

115 Plant viral expression systems can be used for rapid expression of p

116 l-free assays or phenotypic assays in native expression systems can uncover activation of additional

117 In heterologous expression systems, coexpression of NCC with I-1 increas

118 hich the two copies of NS5A from the various expression systems colocalize, the results point to NS3-

119 An insect cell GPIHBP1 expression system confirmed the propensity of GPIHBP1-S1

120 re functionally characterized in a transient expression system consisting of Agrobacterium-infiltrate

121 is and mutant VWF was expressed in different expression systems, COS-7 cells, baby hamster kidney cel

122 Perspectives on how viral expression systems could allow for the development of in

123 on of the coding sequences in a heterologous expression system demonstrated that both were specific r

124 mical studies of recombinant Y3 from a yeast expression system demonstrated the protein is a unique G

125 ene switch system, a chemically induced gene expression system, demonstrated that induction of 9-cis-

126 Translation in a cell-free expression system demonstrates that diminished output fr

127 s, and that subunit linkage can overcome the expression system-dependent preferential assembly of fre

128 Earlier reports have shown that in cellular expression systems diacylglycerol produced during type 1

129 oration combined with an inducible knockdown expression system drives deficits in synaptic GABAA func

130 When expressed by transfection in various expression systems, each of the four alpha subunit isofo

131 Using a heterologous GABA(B)R expression system, electrophysiology, and mutagenesis, w

132 Advances in transient mammalian expression systems enable high yields (>100 mg/liter) th

133 RNA synthetase pairs were inserted into this expression system, enabling the site-specific incorporat

134 Using a retroviral expression system encoding Foxp3 and HLA-DR1 covalently

135 In this study, using an inducible expression system for an miRNA target message, we have d

136 ssly transition into a large-scale mammalian expression system for clinical production.

137 Plants are increasingly being used as an expression system for complex recombinant proteins.

138 e, we address this issue using a recombinant expression system for human dynein coupled to single-mol

139 train of Listeria monocytogenes as a vaccine expression system for LJM11, a sand fly salivary protein

140 d to a RMR motif and cloned into a bacterial expression system for protein production.

141 novel reporters and a doxycycline-regulated expression system for RPB1 In efforts to determine the m

142 Using an inducible expression system for the exosome cargo protein GPRC5B a

143 Reported here is a piggyBac transposon-based expression system for the generation of doxycycline-indu

144 er can be applicable in establishing a novel expression system for the high-level production of forei

145 amster Ovary cells are the most popular host expression system for the large-scale production of huma

146 d electron microscopy and a new heterologous expression system for the lid to delineate the complete

147 nt Anopheles gambiae densovirus (AgDNV) over-expression system for the major malaria vector Anopheles

148 We established a controlled expression system for the parallel functional analysis o

149 Here we present an efficient cell-free expression system for the site-specific incorporation of

150 The Q system is a repressible binary expression system for transgenic manipulations in living

151 on of ENaC is incomplete, we developed yeast expression systems for each ENaC subunit.

152 ince new molecular tools, including improved expression systems for proteins, protein and metabolic e

153 s novel system expands the number of E. coli expression systems for use in recombinant protein produc

154 f truncated hClC-Kb channels in heterologous expression systems fully explains the reduced basolatera

155 Toxicity studies in a prokaryotic dual-expression system grouped the enzymes into high- and low

156 The expression system had a strong effect on the properties

157 cleavage activity in a bacterial carotenoid expression system, had activity similar to lancelet BCO2

158 A mammalian expression system has been developed that permits simult

159 However, no efficient non-tagged apidaecin expression system has been reported, which is the limiti

160 utamate receptor function using heterologous expression systems has complicated the analysis of recep

161 Guanine constraint commonly imposed by gRNA expression systems has little effect on overall cleavage

162 Binary expression systems have revolutionised genetic research

163 In 2 heterologous expression systems (HEK-293T cells and Xenopus laevis oo

164 To this end, using a conditional expression system, here we generated Plk1 transgenic mou

165 etracycline-inducible GFP-S peptide-NFkappaB expression system identified gigaxonin, an ubiquitin E3

166 In this paper, we describe a transient expression system in Arabidopsis mesophyll protoplasts t

167 c clusters, we have developed a heterologous expression system in Aspergillus nidulans .

168 Upon engineering an inducible csrA expression system in B. burgdorferi, controlled hyperexp

169 Using our previously described expression system in Caenorhabditis elegans, we showed t

170 Finally, we use a lentiviral expression system in cultured neurons, where we again fi

171 Thus, heterologous expression system in Dictyostelium cells provides mechan

172 hemical properties of these mutants using an expression system in Escherichia coli that produces homo

173 We established a transient expression system in Nicotiana benthamiana to study deta

174 then expressed using the magnICON transient expression system in plants and their enzymatic properti

175 for a plastid genome and an associated gene expression system in Polytomella spp.

176 ed a hormone-inducible diphtheria toxin (DT) expression system in Saccharomyces cerevisiae which allo

177 Using a heterologous psbA expression system in the green alga Chlamydomonas reinha

178 tissue-specific expression and an inducible expression system in the loss-of-function phyB-9 mutant

179 eloped a robust repressible chloroplast gene expression system in the unicellular alga Chlamydomonas

180 Using a recombinant virus-expression system in tissue culture and in mice, we demo

181 ivo Pv-ALF/phas-GUS (for beta-glucuronidase) expression system in transgenic Arabidopsis thaliana lea

182 Using a heterologous expression system in which human PTEN-GFP is expressed i

183 enzyme, we used a cell-free wheat germ-based expression system in which mRNA encoding FERONIA was co-

184 r the existence of desiccation-specific gene expression systems in P. vanderplanki.

185 xpression of various AMPs using a CaM-fusion expression system, including melittin, fowlicidin-1, tri

186 The capacity of different expression systems, including pseudoviral particles and

187 of mGluR2 in recombinant and native receptor expression systems, increasing the affinity of glutamate

188 tic dissection in situ and in a heterologous expression system indicate that this acid efflux is medi

189 Our cell-based transient expression system is fast and easy to set up and generat

190 The alc gene expression system is one of the most promising chemicall

191 iated iron transport in a yeast heterologous expression system is saturable (Km approximately 14.7 mu

192 This shortcoming of bacterial expression systems is particularly detrimental for the f

193 However, in a heterologous expression system, it acts as a nucleotide antiporter an

194 However, when complex expression systems like hairy roots are used for product

195 metallocofactor formation in the recombinant expression system may be absent, inadequately expressed,

196 Gene therapies with regulated expression systems may provide substantial improvements

197 Cell-free protein expression systems monitored the impact of Zt6 protein o

198 In each expression system, mutation of cysteines in GPIHBP1's Ly

199 ummarize findings obtained from heterologous expression systems, neuroendocrine cells, and reconstitu

200 This study establishes the recombinant expression system of MAF-1 (Musca domestica antifungal p

201 Using the transient expression system of tobacco (Nicotiana tabacum) leaves,

202 An E. coli expression system offers a mean for rapid, high yield an

203 Here, we describe an optogenetic gene expression system optimized for use in zebrafish.

204 translocatable phosphatases in heterologous expression systems or by activation of phospholipase C i

205 ave relied on cultured neurons, heterologous expression systems, or membrane fusion assays in vitro,

206 In a heterologous expression system, OR51E2 trafficked readily to the cell

207 A1 of living rats by means of the lentiviral expression system prior to induction of ischemia afforde

208 We suggest that our E. coli expression system producing functional CRFRs will be use

209 Irrespective of the expression system, receptors resulting from combining th

210 perimental techniques, including conditional expression systems, recyclable markers, RNA interference

211 This cshRNA expression system reduces the coding space required for

212 These gene expression systems rely on a specific promoter region to

213 c receptor signaling pathway in heterologous expression systems remains an unmet challenge.

214 nd that a 2-part nonviral Tet-KRAB inducible expression system repressed expression of a luciferase r

215 In addition, plasmid-based expression systems require DNA replication origins to ma

216 of cmo-miR156, using a virus-based microRNA expression system, restores the early flowering phenotyp

217 dard RM 8670 derived from a murine cell line expression system resulted in detection of 60 mouse HCPs

218 as between Rpg1b and Rpg1r using a transient expression system revealed that AvrB versus AvrRpm1 spec

219 In heterologous expression systems, sevoflurane, isoflurane, and desflur

220 Experiments with heterologous expression systems show that some of the identified Slo1

221 Further analysis using an inducible expression system showed that BAK1 accumulation quickly

222 cterization of duck Piezo2 in a heterologous expression system shows that duck Piezo2 is functionally

223 Using a yeast expression system, specific labeled [(13)C]acetate, and

224 Ser/Thr sites when expressed in a eukaryotic expression system such as baculovirus.

225 Binary expression systems such as GAL4/UAS, LexA/LexAop and QF/

226 e glycans on gp120 was reproduced across all expression systems, supporting this as an intrinsic prop

227 In a heterologous expression system, TbAAT10-1 is selective for ornithine,

228 In a heterologous expression system, TDAG8 potentiated the acid-induced ca

229 racterization of a novel tunable recombinant expression system, termed RiboTite, which operates at bo

230 A conditional gene expression system that is fast-acting, is tunable and ac

231 Isolated protoplasts serve as a transient expression system that is highly representative of stabl

232 enomic imprinting is an allele-specific gene expression system that is important for mammalian develo

233 Here, we describe a bacterial co-expression system that yields milligram quantities of th

234 e libraries in living cells requires plasmid expression systems that suffer from variable copy number

235 It appears therefore that in the oocyte expression system the introduced cysteine residues in ma

236 in the expression of hClC-Kb in heterologous expression systems, the functional consequences of mutan

237 ession of WaaL through use of a chaperone co-expression system then enabled the demonstration of the

238 In heterologous expression systems, these subunits avidly coassemble and

239 In the context of the Xenopus laevis oocyte expression system, this technique yields ion channels wi

240 etically defined cell models and conditional expression systems, this response was shown to be Myc sp

241 ansposon and a Tet-On 3G drug-inducible gene expression system to achieve versatile inducible gene ex

242 d Saccharomyces cerevisiae as a heterologous expression system to assess FAR substrate specificity de

243 crease the sensitivity of the virulence gene expression system to certain sugars that regulate it.

244 We took advantage of the Xenopus laevis expression system to determine the individual characteri

245 Our study utilizes an expression system to dissect the biophysical properties

246 their splice variants, in the same cellular expression system to investigate and compare their elect

247 to palivizumab efficacy using a plant-based expression system to produce palivizumab N-glycan struct

248 (2+) (TRIC) in Drosophila that uses a binary expression system to report Ca(2+)-dependent interaction

249 othesis, we used a diphtheria toxin receptor expression system to selectively ablate these cells from

250 engineered a Cre recombinase-dependent gene expression system to selectively express alpha4 nAChR su

251 employed a doxycycline (DOX)-inducible CTSB expression system to selectively overexpress human CTSB

252 In this study we utilized the Xenopus oocyte expression system to shed light on how CFTR channel acti

253 Here we used an in vitro expression system to study the insertion capacity of hyd

254 extension of this chemically inducible gene expression system to sugar cane opens the door to new op

255 e-based transport assays in two heterologous expression systems to establish a broad substrate recogn

256 d adult Drosophila Using the tissue-specific expression system (UAS)-GAL4, we express Caspase-LOV spe

257 low levels of K(+) currents in heterologous expression systems under physiologically ionic condition

258 ge of cellular phenotypes, yet most cellular expression systems use heterologous cDNA-based vectors w

259 se of NA and M2, clustering depends upon the expression system used.

260 Development of a CHO-based expression system using genes encoding human butyrylchol

261 tch-clamp technique on either a heterologous expression system using giant spheroplasts of MJF465 Esc

262 SD-associated SCN2A variants in heterologous expression systems using whole-cell voltage-clamp electr

263 creening was conducted in the Xenopus oocyte expression system, using quantitative swelling and two-e

264 n in specific cell populations has made gene expression systems valuable tools in developmental biolo

265 A heterologous Lactococcus lactis expression system was used to express SdrF and its ligan

266 The synthetic gene expression system was validated with two reporter genes

267 e outer hair cells (OHCs) and a heterologous expression system, we also found that this calcium-oblig

268 Using a recombinant expression system, we demonstrate that CO inhibits peak

269 and alpha4beta2 nAChRs in the Xenopus oocyte expression system, we demonstrate that inhibition by MLA

270 sing an Escherichia coli heterologous sterol expression system, we demonstrate that substitution of f

271 Using an inducible expression system, we discovered that age-onset phenotyp

272 Using a transient heterologous cell expression system, we find that the transport activities

273 Using an inducible expression system, we found that IRF4 directly induced a

274 In a heterologous expression system, we found that one of the PRKDC mutati

275 a regulatable (doxycycline-repressible) gene expression system, we found that the pathogenic PPA2 mut

276 Using a binary gene expression system, we generated transgenic mice expressi

277 Using a recombinant expression system, we have studied homogeneous samples o

278 otypical bitter substances in a heterologous expression system, we identified cognate compounds for 2

279 Using a baculovirus expression system, we previously found that DSP-PP is ac

280 Here, using a transient plant expression system, we show that AsCYP51H10 is a multifun

281 Using a lysozyme-M-cre (LysMcre) expression system, we show that deletion of autophagy-re

282 esponsive modification of plasmid-based gene expression systems, we have demonstrated optochemical ac

283 onal readouts in heterologous and endogenous expression systems, we identified the UDP receptor P2Y6

284 In heterologous and endogenous expression systems, we studied the role of ERp44 and its

285 miR-449b through a doxycycline (dox) induced expression system were used as nuclear donor cells for S

286 nd a plant (Nicotiana benthamiana) transient expression system were used to test the ability of Arabi

287 Transient expression systems were used to characterize Zt6, and mu

288 ed calcium (Ca(2+)) channels in heterologous expression systems were used to determine the modulation

289 forms, and among studies, different cellular expression systems were used.

290 ix and may be useful in analogous designs in expression systems where the incorporation of hydroxypro

291 ressed using the baculovirus/Sf9 insect cell expression system, whereas others have been recalcitrant

292 ld and purity of HBc particles in an E. coli-expression system, which are fully characterised and sui

293 ea Mosaic Virus hypertranslational "CPMV-HT" expression system, which provides high yields of recombi

294 -length PfMyoA with the baculovirus/Sf9 cell expression system, which required a UCS (UNC-45/CRO1/She

295 function, we screened an AIRE-dependent gene-expression system with a genome-scale lentiviral shRNA l

296 analysed in an in vivo protoplast transient expression system with a novel mini-exon splicing report

297 nucleopolyhedrovirus (BmNPV)-based silkworm expression system with its innate signal peptide, bombyx

298 TSTA exendin-4 expression system with SP and ABP polymer has a potentia

299 optimized pyrrolysyl-tRNA synthetase/tRNACUA expression system, with optimized tRNA expression for ma

300 re however formed with Kv2.1 in heterologous expression systems, with both alpha-subunit genes expres