ライフサイエンスコーパス: expression vector

1 ner, as a result of the design of a novel co-expression vector.

2 bone marrow cells transfected with a Prdm14 expression vector.

3 ized and cloned into a small interfering RNA expression vector.

4 ulated by co-transfection with the C/EBPbeta expression vector.

5 in vivo using a transposon-based eukaryotic expression vector.

6 transfection of an exogenous PPARbeta/delta expression vector.

7 7 cells stably transfected with an aromatase expression vector.

8 ary DNA (cDNA) using pc DNA3.1 (+)/Myc-His A expression vector.

9 cells, which lack EGFR expression, with EGFR expression vector.

10 stem (ES) cells were transfected with a Tlx3 expression vector.

11 NA induction by a constitutively active CHK2 expression vector.

12 poprotein genes were cloned into a mammalian expression vector.

13 a cells were stably transfected with a DAbR1 expression vector.

14 ompatible and reading frame-compatible plant expression vector.

15 osiphum padi virus (RhPV) into a baculovirus expression vector.

16 and TIMP2 cDNA were cloned into a mammalian expression vector.

17 over cells transfected with the parent eGFP expression vector.

18 encoding Firefly luciferase in a dicistronic expression vector.

19 rexpressing PKC-delta using a Zn2+ inducible expression vector.

20 falciparum and cloned this into a mammalian expression vector.

21 MP-9 promoter by a constitutively active MEK expression vector.

22 y reversed in cells transfected with an ARNT expression vector.

23 lls and in cells transfected with an E1B-55K expression vector.

24 erexpressed via transient transfection of an expression vector.

25 nally into a zero background BsaI/ccdB-based expression vector.

26 inal end to generate CPD-MAGE-A3 in a pQE-70 expression vector.

27 ovel hypoxia and hepatoma dual specific gene expression vector.

28 nalyses using a zucchini yellow mosaic virus expression vector.

29 epG2 cells stably transduced with lentiviral expression vectors.

30 were transfected with EpoR and/or RNF41 gene expression vectors.

31 ZF-TF that can be transferred to a number of expression vectors.

32 FK506 and transfected with GRbeta and FKBP51 expression vectors.

33 and independent of cloning dsRNAs into plant expression vectors.

34 fied by PCR and cloned into Escherichia coli expression vectors.

35 ughput transfer of ORF inserts into suitable expression vectors.

36 e constructed using product similarity among expression vectors.

37 pid and efficient cloning of gRNA pairs into expression vectors.

38 tion of either of two dominant-negative CREB expression vectors.

39 and SUMO-1 (small ubiquitin-like modifier-1) expression vectors.

40 ing p13(II), using both transient and stable expression vectors.

41 nically or with adeno-associated virus (AAV) expression vectors.

42 lls that overexpressed GATA6 from lentiviral expression vectors.

43 the TF sequences into a number of different expression vectors.

44 cable means to limit off-targeting from RNAi expression vectors.

45 e relative concentrations of the Flp and Int expression vectors.

46 ave practical implications for the design of expression vectors.

47 d cell lines and cell lines transfected with expression vectors.

48 mal RNA and transcripts of their baculoviral expression vectors.

49 genes are typically transferred to mammalian expression vectors, a major rate-limiting step in the it

50 Transfection of HUVECs with MCPIP expression vector activated the expression of cdh12 and

51 e recombinases-the amount of the recombinase expression vectors added at transfection-and on the orde

52 pression assays (agroinfiltration) and virus expression vectors (agroinfection) to express functional

53 hain variable regions were subcloned into an expression vector allowing the production of recombinant

54 iently transfected with a wild-type (WT) p53 expression vector along with a luciferase reporter conta

55 with the LmGT4 gene on a multicopy episomal expression vector also reverted these phenotypes, confir

56 ma 2008 cells were transfected with an hREV1 expression vector and 4 sublines developed in which the

57 bly expressing a Tet-on RORalpha or RORgamma expression vector and a ROR-responsive element (RORE)-LU

58 cloning of the TM1418-TM1419 operon in pBAD expression vector and confirmed to function in the crude

59 s stably transfected with a HIF-1alpha siRNA expression vector and found that HIF-1alpha mRNA silenci

60 th the pLucRLuc:p53/Wrap53beta bidirectional expression vector and known p53 transcriptional regulato

61 The DNA encoding lpt3 was cloned into an expression vector and Lpt3 was purified.

62 i DE3 strain using a T7 promoter-based pET30 expression vector and nickel affinity column chromatogra

63 taVIII was examined by subcloning it into an expression vector and raising an antibody specific to PK

64 ESRP1 expression vector and small interference RNA (siRNA) tar

65 ies in soluble form from an Escherichia coli expression vector and the enzyme stripped the PDGA capsu

66 es were cloned into a tetracycline-regulated expression vector and transfected into wild-type cells.

67 cNr, the coding region was subcloned into an expression vector and transformed into Escherichia coli.

68 Notably, when agaF+ was cloned into an expression vector and transformed into six spinach isola

69 collection was transferred into the relevant expression vector and used to produce a protein microarr

70 Compared to conventional plasmid-based expression vectors and donor templates, we show that com

71 he viral glycoproteins and CD163 receptor in expression vectors and examined their expression and int

72 reproducible protocol for electroporation of expression vectors and morpholino oligonucleotides into

73 erapeutic agents, short interfering RNA, DNA expression vectors and proteins to different types of ce

74 irus replication and lead to improvements in expression vectors and recombinant vaccines.

75 hich the mutant libraries are transferred to expression vectors and screened for phenotypes of intere

76 and knockdown were performed using specific expression vectors and siRNA technology, respectively.

77 inatorial assembly of functional (multi)gene expression vectors and their efficient and specific targ

78 lified by RT-PCR and nested PCR, cloned into expression vectors and transfected into a human cell lin

79 olactin beta was inserted into two different expression vectors and transfected into E. coli or P. pa

80 gated for their applications as vaccines and expression vectors and, more recently, because of concer

81 trate, using an miR-10b synthetic precursor, expression vector, and antisense oligonucleotide, that m

82 ll-length cDNA was cloned into a baculovirus expression vector, and recombinant Aed a 3 (rAed a 3) wa

83 The MHC-I cDNA was cloned into a mammalian expression vector, and stable B78H1 cell lines were gene

84 althy T cells by transfection with CREMalpha expression vector, and Syk expression and phosphorylatio

85 were cloned individually into a prokaryotic expression vector, and their gene products were purified

86 atient-derived serum samples, cloned into an expression vector, and used to generate viral pseudopart

87 Once the proper expression vectors are cloned, our protocol should allow

88 Virus-based expression vectors are commonplace tools for the product

89 Baculovirus expression vectors are used for introducing the AAV gene

90 Multicloning sites (MCSs) in standard expression vectors are widely used and thought to be ben

91 free web-based application to design protein expression vectors, artificial gene networks and other g

92 nhibits expression of exogenous MDM2 from an expression vector as long as the vector contains an AU-/

93 These genes were cloned into three expression vectors as native sequences and as N-terminal

94 Optimization of codon use in expression vectors, as expected, restores efficient prot

95 Fragments are then combined with linearized expression vectors, assembled in vitro as part of a sequ

96 er, IL-21 in a synergistic manner with IL-15 expression vector augmented the vaccine-induced recall r

97 This method utilizing the antibody expression vectors, available at Addgene, has many appli

98 f fully assembled ZFNs into Escherichia coli expression vectors, bacterial lysates were found to be m

99 Here, a series of expression vectors based on Oryza sativa MIR390 (OsMIR39

100 ribes the construction of a set of versatile expression vectors based on the In-Fusion cloning enzyme

101 cialties, the design of application-specific expression vectors becomes the new norm.

102 tudying EBV capsid assembly, the baculovirus expression vector (BEV) system was used to express the c

103 e cell is infected with a single baculovirus expression vector (BEV).

104 adenoviral transduction of dominant negative expression vectors blocked IL-17-mediated CRP induction.

105 d vector that can be rapidly converted to an expression vector by a simple restriction enzyme digesti

106 Tobacco mosaic virus (TMV)-based transient expression vectors can express very high levels of forei

107 linical studies is developing a suitable CAR expression vector capable of genetically modifying a bro

108 Considerations of expression vector choice, modifications to sequence, tro

109 A mammalian expression vector coding for FLAG-tagged human ADAMTS4 w

110 We initially constructed a beta-globin expression vector composed of the hybrid cytomegalovirus

111 targeted exosomes through transfection of an expression vector, comprising an exosomal protein fused

112 Transfection of CHO cells with a GPIHBP1 expression vector confers on cells the ability to bind b

113 Fusion expression vectors contained either bacterial alkaline p

114 cotransfected into TM cells with a mammalian expression vector containing a dominant-negative mutant

115 ate which is in turn hybridized to a plasmid expression vector containing an 11-bp target sequence.

116 00A) was transfected with the p426 GPD yeast expression vector containing DNA encoding the wild-type

117 s, we stably transfected INS-1 cells with an expression vector containing the gene for the DNA repair

118 Using an expression vector containing the minimal tissue factor p

119 An expression vector containing the soybean phytase cDNA co

120 Co-transformation of an expression vector containing this gene and pSUPAR6-L3-3S

121 Expression vectors containing constitutively active CaMK

122 Two tissue specific fluorescent expression vectors containing either a GFP or mCherry tr

123 By delivering expression vectors containing either wild-type or mutant

124 ently transfected with combinations of human expression vectors containing genes for TLR2, TLR1, and

125 ategy described here to synthesize mammalian expression vectors containing site-specific cisplatin IC

126 Cotransfection of RNA and an I-SceI expression vector demonstrated insertion of RNA-derived

127 A new protein expression vector design utilizing an N-terminal six-his

128 In this work, a new GFP expression vector designated pUAS-Neostinger was constru

129 Protein expression vectors designed for large-scale protein prod

130 Retroviral transduction with a Hoxa-9 expression vector dramatically enhanced the cytokine-dri

131 ted with an ARHI/luciferase reporter and E2F-expression vectors, E2F1 and 4 reduced ARHI promoter act

132 process, we constructed a series of plasmid expression vectors, each containing a different type II

133 pression of ExoS in HeLa cells by eukaryotic expression vector effectively caused apoptosis in an ADP

134 Transfection of an expression vector encoding constitutively active IKK2 in

135 e inflammasome adaptor ASC, transfected with expression vectors encoding A1PI-M or A1PI-Z.

136 Immunization with plasmid expression vectors encoding hemagglutinin (HA) elicited

137 Expression vectors encoding mutants of the Ras/mitogen-a

138 Cotransfections with expression vectors encoding NF-kappaB subunits were carr

139 We constructed expression vectors encoding short fusion peptides corres

140 -transfections was performed using mammalian expression vectors encoding specific glycosyltransferase

141 a series of co-transfections using mammalian expression vectors encoding specific glycosyltransferase

142 for gp80 independence of vIL-6, a series of expression vectors encoding vIL-6/hIL-6 chimeric and sit

143 transfection of a luciferase construct with expression vectors following MITF siRNA revealed that TF

144 yeast to design a single genomic capture and expression vector for antibiotic production in Bacillus

145 epatocytes were transduced with a lentiviral expression vector for Cd47 using a refined in vitro tran

146 zyme sites for inserting the product into an expression vector for cloning purposes.

147 Cloning of the gene in an expression vector for ex vivo cell-cell fusion and pseud

148 expression, PC12 cells were transfected with expression vector for full length or truncated inactive

149 hage display are typically subcloned into an expression vector for further biochemical characterizati

150 Introduction of an expression vector for KLF15 short hairpin RNA, together

151 nd metastatic by stable transfection with an expression vector for OPN to identify RAN GTPase (RAN) a

152 ransfection of noninvasive R37 cells with an expression vector for RAN resulted in increased anchorag

153 id (MIP) is an alternative, robust transgene expression vector for reprogramming.

154 Transfection of an expression vector for S100P into a benign, nonmetastatic

155 HepG2 cells transfected with an expression vector for the active nuclear form of SREBP-1

156 a one-step cloning, into a binary multigene expression vector for transient or stable coexpression w

157 Finally, we modified existing expression vectors for 19 epitope-tagged human WNT prote

158 n of fluorescent CNS reporter constructs and expression vectors for constitutively active signaling p

159 We report the generation of versatile expression vectors for detection and isolation of protei

160 transport genes and Gateway((R))-compatible expression vectors for functional analysis and subcellul

161 Introduction of expression vectors for miR-203 into keratinocytes that s

162 Introduction of expression vectors for miR-424 reduced both the levels o

163 be easily adapted for developing specialized expression vectors for other organisms, zero background

164 We have developed new RNA polymerase II expression vectors for RNAi, designated SIBR vectors, ba

165 ein we report development and testing of new expression vectors for Sindbis, Chikungunya, and eastern

166 ric mouse-human genes were cloned into plant expression vectors for stable nuclear transformation of

167 We built cDNA expression vectors for the 13 previously undescribed mis

168 ith a CYP3A4 proximal promoter construct and expression vectors for the NFI isoforms NFIA1.1, NFIB2,

169 Here, we report construction of new expression vectors for two Old World arthritogenic alpha

170 ovide evidence that the use of hairpin siRNA expression vectors for uPAR and MMP-9 may provide an eff

171 in mice in the absence of tumor cells using expression vectors for VEGF-A(164).

172 nzymes, HEK293-F cells were transfected with expression vectors for visual cycle proteins and co-tran

173 nsfection of an IL13 reporter construct with expression vectors for wild type or mutant CREB and ATF-

174 -/-) MEFs that were infected with adenoviral expression vectors for Zmiz2.

175 ibe a detailed protocol for constructing ZFN expression vectors, for generating and introducing ZFN-e

176 In contrast, genomic DNA expression vectors generate physiologically-relevant lev

177 Expression vectors generated by in vitro recombination o

178 oding hemagglutinin-tagged, full-length PUMA expression vector (HA-PUMA), PUMA lacking the Bcl-2 homo

179 lin G (IgG) 5H2, originally produced from an expression vector, has been shown to be a variant contai

180 MBP-facilitated crystallization, a series of expression vectors have been designed with either a shor

181 Improved expression vectors have been tested for protein expressi

182 strate the feasibility of miRNA targeting of expression vector helper functions that are provided in

183 ters was significantly augmented by an LRH-1 expression vector in a co-transfection assay.

184 strated by targeted mutagenesis of an NKX3.1 expression vector in a SMGA reporter assay.

185 As to be assembled into a single baculoviral expression vector in only two steps.

186 hree overlapping fragments from a lentiviral expression vector in the epidermis of living skin equiva

187 lytic form of viral replication with a BZLF1 expression vector in the presence and absence of various

188 First, we built an expression vector in which the Oct3/4 promoter drives th

189 Using expression vectors in 293T cells or Jurkat T cells, we s

190 Coexpression of WT TLR4, CD14, and MD-2 expression vectors in HEK293T cells was first optimized

191 athogens that have been developed as protein expression vectors in insect cells and as transduction v

192 by transfection with C/EBPbeta and NF-kappaB expression vectors in the presence or absence of IL-1bet

193 Gene transfer using an adenoviral hRAMP1 expression vector increased the maximal production of cA

194 Transfection of HUVECs with an MCPIP expression vector induced angiogenesis-related genes and

195 stability, we found that transfection of ZFN-expression vectors induced up to a 15-fold increase in c

196 and prostate cancer cells with a DLC-3alpha expression vector inhibited cell proliferation, colony f

197 transfected with a doxycycline-regulated AHR expression vector, inhibition of AHR expression causes a

198 A Ski siRNA expression vector injected into nude mice resulted in a

199 Using YFP-fusion gene expression vectors, interaction between 14-3-3zeta and c

200 initransposon that can function as a protein expression vector into a plasmid that contains the open

201 of CRC cells stably transfected with a Cten expression vector into nude mice and (b) testing a serie

202 By electroporating a cre-recombinase expression vector into the cortex of E13.5 embryos carry

203 into the node, and electroporation of a GFP expression vector into the midline neural plate, reveale

204 cell lines by stable transfection of ERalpha expression vectors into ER- breast cancer BCap37 cells.

205 16-specific DNA methyltransferase (P16-dnmt) expression vector is designed using a P16 promoter-speci

206 The newly created expression vector is expected to enhance transgene expre

207 delivery systems and hypoxia-regulated gene expression vectors is the most important factors.

208 Introducing a LSD1 expression vector lacking the miR-137 recognition site r

209 reversed by re-expression of FUNDC1 and NIX expression vectors lacking the miR-137 recognition sites

210 nt into a heterologous intron in a mammalian expression vector markedly reduced the expression of the

211 Finally, we demonstrate that the ble-2A expression vector may be used to fluorescently label an

212 lower in KO mice than in cell lines via RGN expression vector-mediated genome editing.

213 Introducing an expression vector of Tlx or cyclin D1 that lacks the let

214 rix indicating the cosine similarity between expression vectors of genes in this database.

215 For hippocampal CA3-->CA1 synapses, AAV expression vectors of serotype 1, 5, and 8 led to light-

216 a/K-ATPase alpha1 knockdown PY-17 cells with expression vectors of wild type or mutant alpha1 carryin

217 Episomal gene expression vectors offer a safe and attractive alternati

218 -008a were expressed from the same mammalian expression vector, only Ser5-001 was stably expressed, w

219 th mu-36p66ShcA (micro-36) dominant negative expression vector or isoform-specific p66-small interfer

220 e transfected cells with a wild-type GPIHBP1 expression vector or mutant GPIHBP1 vectors in which spe

221 gether, our findings suggest that miR-143 re-expression vectors or selective agents directed at miR-1

222 ecific Fabpl promoter-driven Cre recombinase expression vector packaged into transferrin-coated nanop

223 We cloned the MnSOD gene, sodA, using the expression vector pBAD, overexpressed the product in Esc

224 A full-length Cx43 cloned into mammalian expression vector pCI-neo was used to transfect testes o

225 alpha3(IV) chain was cloned into a mammalian expression vector, pCI-neo, with and without a collagen

226 1 site in the Gateway (Invitrogen) bacterial expression vector pDEST17, necessary for in vitro site-s

227 n inhibiting osteosarcoma, we constructed an expression vector pEGFP-ING4 and transfected the human o

228 C-terminus, were cloned into the recombinant expression vector pET-28a-PelB and auto-induced in Esche

229 aryotic expression plasmid using prokaryotic expression vector (pET-28a(+)) and converted it to the c

230 entiviral TALE-Kruppel-associated box (KRAB) expression vector platform that enables knockdown of mul

231 They are derived from the broad host range expression vector pNSGroE and have several advantages (i

232 strategies for developing recombinant RVs as expression vectors, potentially leading to next-generati

233 tic function by cloning it into a lentiviral expression vector prior to introduction into the human N

234 he liver of C57BL/6 mice using an adenoviral expression vector produced significant decreases in ADMA

235 c cDNAs with a vaccine strain background and expression vector properties.

236 Co-transfection of APOBEC3A with a TRIB3 expression vector reduced nuclear DNA editing whereas si

237 phorbol 12-myristate 13-acetate or an egr-1 expression vector rescued BKS-2 cells from BCR signal-in

238 f SKI-1/S1P-deficient cells with a SKI-1/S1P expression vector restored release of infectious virus (

239 Complementation of the mutants by an aroA' expression vector restored the wild-type phenotype.

240 eroidogenic COS-1 cells with P450c17 and p38 expression vectors showed that p38alpha, but not p38beta

241 Transfection of cell lines with ZNHIT3 expression vectors showed that the PEHO syndrome mutant

242 Use of a dominant-negative Smad3 (Smad3 DN) expression vector, Smad3 small interfering RNA, and inhi

243 Using this TMV expression vector, some foreign proteins were expressed

244 and light-chain DNAs into a single mammalian expression vector, starting from DNAs encoding the desir

245 he incorporation of the DNMT1-specific shRNA expression vector, suggesting a growth defect with reduc

246 Expression vector synthesis and purification of the zymo

247 and Nanog in Sf9 cells using the baculovirus expression vector system (BEVS).

248 prompted the development of a bidirectional expression vector system (pLucRLuc) that is capable of m

249 , we have taken advantage of the baculovirus expression vector system for production of endotoxin-fre

250 apsids in insect cells using the baculovirus expression vector system.

251 JAK2 were overproduced using the baculoviral expression vector system.

252 nomechanics, we designed a family of cloning/expression vectors, termed pFS (plasmid for force spectr

253 nduced phenotypes in vivo, we describe siRNA expression vectors that allow coexpression of one or mor

254 Here we have used these features to design expression vectors that can stably express virtually any

255 he roles of PKCbeta1 and beta2, we used cDNA expression vectors that encode wild-type and constitutiv

256 ly, we have generated a series of multi-copy expression vectors that use resistance to nourseothricin

257 on of opsin/iNOS transgene in the CMVSport 6 expression vector, the 4.4 kb Acc65I/Xhol mouse rod opsi

258 however, when treated with FGFR1OP2/wit3.0 -expression vector, the skin wound closure was significan

259 the fragment cloned into other targeting or expression vectors, the multi-cloning sites of three shu

260 clone PCR products directly into destination/expression vectors, thereby bypassing the requirement fo

261 ransfected with either empty vector or DKK-1 expression vector to block Wnt activity.

262 r Huh7 cells transfected with empty or MAT1A expression vector to establish tumors.

263 to enhance cellular uptake and a linearized expression vector to induce enhanced expression of brain

264 human Cu,Zn-SOD gene with TAT in a bacterial expression vector to produce a genetic in-frame His-tagg

265 a lentiviral-based short hairpin RNA (shRNA) expression vector to stably reduce c-Myc expression in a

266 ct genetic approaches were used to construct expression vectors to achieve SAP overexpression, and bo

267 We designed lentiviral re-expression vectors to achieve targeted re-expression of

268 nd epitope (Flag)-tagged wild-type (WT) TLR4 expression vectors to permit a direct comparison of WT a

269 ssing IFN-beta expression, we used transient expression vectors to test the abilities of a diverse co

270 were co-transfected with GAL3ST3 and B3GNT7 expression vectors, transfected cells weakly expressed H

271 e Nurr1 overexpressed SH-SY5Y cells by Nurr1 expression vector transfection rescued the lactacystin-i

272 r recessive null conditions via a viral gene expression vector transferring a cDNA encoding an enzyme

273 Expression vectors used in different biotechnology appli

274 using mixed infections of a wild-type Trpv1 expression vector vTTHR and a nonfunctional 'poreless' T

275 amplified by homology-based cloning, and its expression vector was constructed for transient expressi

276 A zinc-inducible human antizyme gene expression vector was transfected into UM1 human oral sq

277 A mouse embryo cDNA library in a yeast expression vector was used to transform sak1Delta tos3De

278 Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in v

279 Mutations within an HIV-1 IN bacterial expression vector were based on Tn5 and resulted in 57-b

280 type and A205T mutant of the STK11/LKB1 gene expression vectors were created and transfected into RPM

281 High throughput-ready expression vectors were developed that permit rapid clon

282 Based on these findings, dual gene expression vectors were generated, producing an optimal

283 ere introduced into the DHBV genome, genomic expression vectors were transfected into cells which sup

284 oral regulation, Cre/loxP-mediated inducible expression vectors were used in combination with a vecto

285 ide modulator of splicing (bombesin) and SRp expression vectors were used to modulate SRp levels and

286 scFv antibodies (after transfer to mammalian expression vectors) were screened for viability in a neu

287 -viral transfection of a single multiprotein expression vector, which comprises the coding sequences

288 y CD4(+)CD25(-)FOXP3(-) T cells with a FOXP3 expression vector, which resulted in prompt production o

289 commercially available fluorochromes to DNA expression vectors while retaining biological function.

290 It is proposed that this new transient expression vector will be a useful tool for expressing r

291 The optimized FIV-based RNAi expression vectors will find broad use given the extensi

292 me of human BCO1 was cloned into the pET-28b expression vector with a C-terminal polyhistidine tag, a

293 promoter, and that cotransfection of an IRF8 expression vector with an Mdm2 reporter construct stimul

294 Its gene was cloned into an expression vector with an N-terminal polyhistidine tag,

295 ore, the hypoxia/hepatoma dual specific gene expression vector with the Epo enhancer and AFP promoter

296 To test this, we constructed new expression vectors with an artificially synthesized NTR

297 (family Togaviridae) have been developed as expression vectors with demonstrated potential in vaccin

298 AT/enhancer binding protein beta (C/EBPbeta) expression vectors with or without resistin.

299 modules and transfer of assembled arrays to expression vectors without the need for specialized know

300 infection of cells transfected with an ICP22 expression vector yielded ICP22 protein that was modifie