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1 sDNA-T concentration over the range from 1.0 fM to 1.0nM, with a detection limit of 0.47 fM, can be o
3 eprotonated prior to Zn(II) binding, the 1.0 fM Zn(II)-GGG Kd value reflects a Zn(II) complexation re
6 ing (~435 fold) the detection limit from 3.0 fM (without NSs labeling) to 6.9 aM (with NSs labeling).
10 se concentrations ranged from 0.76 fM to 6.0 fM showed that cluster number has a linear relationship
11 ing its detection at a concentration of 0.01 fM, five orders of magnitude lower than that detectable
13 e with the detection limit estimated as 0.03 fM (S/N=3.0) and 0.08 fM (S/N=3.0) for 20-mer ssDNA and
16 OD of 1100 bacteria per 25 muL sample (0.074 fM), and (b) water from the Charles River, with an LOD o
17 imit estimated as 0.03 fM (S/N=3.0) and 0.08 fM (S/N=3.0) for 20-mer ssDNA and for dsDNA (PCR product
19 ient of 0.999, a detection limit between 0.1 fM and 0.5 fM, and a linear dynamic range of over 500-fo
20 (ssDNA) concentrations in the range from 0.1 fM to 0.1 nM with a low detection limit of 62.41 aM, and
21 ch, the detection limit of miRNA-122 was 0.1 fM via direct readout, with a wide detection range from
23 +) (Site I K(a)=1.3 fM(-1), Site II K(a)=1.1 fM(-1)), in conjunction with reversible direct Cu(+) tra
27 otein, reaching a detection limit of 4 +/- 1 fM in buffer and 10 +/- 2 fM in 10-fold diluted nasophar
29 The Scano-miR system was able to detect 1 fM concentrations of miRNA in serum with single nucleoti
30 ors were able to detect glycoproteins from 1 fM (Con A), 10 fM (Ricinus communis agglutinin (RCA), or
33 /- 2.1% for target DNA concentrations from 1 fM to 10 fM, while a capacitance increase was observed w
34 20s and exhibits a large linear range from 1 fM to 10 pM, with a limit of detection (LOD) of 152.93 a
35 n of 0.37 fM and a wide dynamic range from 1 fM to 100 nM along with clear distinction from mismatche
38 complementary strands at concentrations of 1 fM, 1 pM, and 1 microM, both separately and sequentially
40 ted within the dynamic range from 10 aM to 1 fM and detect as low as 2 aM of miR-122 ( approximately
41 235, 419, and 1613 nucleotides at 1 pM to 1 fM and found that the LOD decreased as DNA length increa
45 ion sensitivity in sandwich assays down to 1 fM--a three-order-of-magnitude improvement over most rep
46 itivity allowed us to detect unprecedented 1 fM Hg ions in 20 min in field samples treated by simple
48 o detect glycoproteins from 1 fM (Con A), 10 fM (Ricinus communis agglutinin (RCA), or 100 fM (SNA) w
54 in GABA(A)-mediated inhibition at between 10 fM and 10 nM, a response normally associated with benzod
55 as biphasic, being dose dependent between 10 fM and the peak effect at 10 pM, and inversely related t
57 mic range spanning 6 orders of magnitude (10 fM-1 nM) with a limit of detection of less than 10 attom
58 bodies, we establish a detection limit of 10 fM for the protein IL-2, 150 times more sensitive than t
61 osited gold layer, the detection limit of 10 fM R6G solution concentration with uniform SERS effect a
67 y showed excellent detection sensitivity (10 fM) and specificity and was demonstrated for quantitativ
68 ssociation constant: biotin/streptavidin (10 fM) and HER2/HER2 antibody (0.44 +/- 0.07nM), respective
69 With this assay, detection limits down to 10 fM and 1 pM were achieved for proteins and target DNA, r
70 t insulin with limit of detection down to 10 fM in both buffer solution and diluted human serum witho
71 or target DNA concentrations from 1 fM to 10 fM, while a capacitance increase was observed when H5 ta
77 cted at initial concentrations as low as 100 fM by using a combination of field-amplified injection a
81 ere detected at concentrations as low as 100 fM, corresponding to <1 muL of perfluorocarbon per liter
83 e to enable human CDK2 to be detected at 100 fM or 5 pg/mL, well within the clinically relevant range
84 evels of circulating TF, whole blood (+/-100 fM added TF) was tested under static and flow conditions
87 limits were demonstrated down to nearly 100 fM, which may be low enough to identify certain genetic
88 We demonstrated detection of trace of 100 fM Alexa Fluor 488 (signal-to-noise ratio of 11) with a
92 al system, with a low detection limit of 100 fM, which is approximately 5 orders of magnitude lower t
93 M (Ricinus communis agglutinin (RCA), or 100 fM (SNA) with a linear range spanning 6 (SNA), 7 (RCA),
95 10,000 ssDNA molecules corresponding to 100 fM thrombin in solution) by a subsequent surface RNA tra
96 of eight transistors--is achieved with a 100-fM sensitivity, on par with optical DNA microarrays and
97 probes; the insulin detection limit was 128 fM with a dynamic range of over 4 orders of magnitude in
98 idly and reliably detected up to 1 pg/mL (13 fM) concentration on PNA electrode but, as expected, yie
99 ter Hg(0) concentrations ( approximately 130 fM) observed in the Pacific intertropical convergence zo
102 Sea and found low concentrations (39 +/- 16 fM) above the halocline and high concentrations in anoxi
103 35.4 nl assay volume) for target DNA and 16 fM (338 molecules) for target RNA after 1h on-chip hybri
104 with LOD values of 650 fM (160 amol) and 190 fM (50 amol) for the event-specific and the taxon-specif
105 n limit of 4 +/- 1 fM in buffer and 10 +/- 2 fM in 10-fold diluted nasopharyngeal swabs, which is com
106 he device is able to selectively detect 36.2 fM of EGFR in the total protein solution of 0.1 ng/ml ex
107 t the amperometric detection of glucose at 2 fM concentration in a physiological buffer solution at 1
108 coma cells are also sensitive to fM-GAi; (2) fM-GAi drugs only display inhibitory activity against HG
110 tokine interleukin-2 concentrations from <20 fM to >200 pM were demonstrated, surpassing the conventi
112 blood at a concentration as low as 16 to 20 fM results in pronounced acceleration of clot formation.
114 cids, with limits of detection as low as 200 fM, were achieved using a capillary format with a total
119 concentration detection limits of 270 +/- 25 fM and mass detection limits of 150 +/- 15 zmol for Chro
120 nsor provided a very low detection limit (25 fM, 0.25 attomol in 10muL sample) for miRNA-21 without a
121 he case of SPR-PI, the detection limit of 25 fM for nanoparticle-enhanced SPR-PI is approximately 20
122 eously detected at concentrations down to 25 fM using a three-sequence hybridization format that empl
124 ation, with a limit of detection down to 260 fM (260 x 10(-15) M), two orders of magnitude higher tha
125 highest known engineered affinity (K(d)=270 fM) to its high affinity wild-type (K(d)=700 pM) through
126 e a limit of detection (LOD) of 10 fg/ml (28 fM) and are able to detect catalytic activity of thrombi
131 ty of these sites for Cu(+) (Site I K(a)=1.3 fM(-1), Site II K(a)=1.1 fM(-1)), in conjunction with re
132 values were calculated and found to be 26.3 fM and 62.04 m degrees , respectively, for the immobiliz
136 its of detection [LODs] ranging from 1 to 30 fM) and high specificity (distinguishing miRNAs with a s
140 and multiplexing capabilities, detecting 326-fM concentrations of SERS nanoparticles and unmixing 10
142 hibitor of AChE, TZ2PIQ-A6 with a K(d) of 33 fM, did not distinguish between the active and OP-inhibi
144 ic range of EV concentration ranging from 35 fM to 35 pM, which matches the typical range of EV conce
145 ay achieved a limit of detection (LOD) of 35 fM and signals were detectable with analyte concentratio
147 is increased by a factor of >10(8), from 36 fM to >4 microM, and the selectivity factor for ANG is n
149 n of miRNA with a limit of detection of 0.37 fM and a wide dynamic range from 1 fM to 100 nM along wi
150 le detection yielded a detection limit of 39 fM (831 molecules in 35.4 nl assay volume) for target DN
154 , the limit of detection of RNA is about 0.4 fM, which is 10 000-fold more sensitive than conventiona
155 riation (CV) across a range from nearly 27.4 fM to 1.7 pM using the described collection method.
158 ncentration detection limits were 520 +/- 40 fM and mass detection limits were 310 +/- 30 zmol with p
159 ) to approximately 7 pg/mL (approximately 40 fM analyte antibody concentration), and also expands the
160 were observed when as little as 40 amol (400 fM) of the desired target was present in the hybridizati
162 ed (not extrapolated) detection limit of 400 fM, which is among the best reported for single-step ele
163 es a limit of detection of 1.2 pg mL(-1) (42 fM) PSA in 25% blood serum, which is about ten times mor
165 ited triplet state concentrations of </=0.45 fM produced by UVA and visible light irradiation of natu
166 and EHI_182030 (10 pg/mL, corresponding 453 fM) with high specificity has been achieved, employing t
169 equilibrium dissociation constant K(d) = 48 fM and slower dissociation kinetics (half-time > 5 days)
174 tion for this assay was determined to be 2.5 fM, and this is the first demonstration of a bar code-ty
176 ngle molecules at concentrations as low as 5 fM, which is approximately a 10(3) reduction in the limi
182 magnitude by direct molecule counting; a 50 fM dual-labeled model sample can be detected with 99.5%
185 Proteins with concentrations as low as 50 fM were detectable with 30 min of preconcentration time.
187 ling was observed when two bismuth atoms (50 fM radiolabeled antibody) were initially bound onto the
190 At present, the sensitivity limit of 50 fM is determined by the encounter rate of the labeled an
195 e to femtomolar concentrations of miRNA (500 fM), has a three-log linear dynamic range and is capable
196 of human thrombin at a concentration of 500 fM; the appropriate thrombin aptamer for the sandwich as
198 FI-SPR provides a detection level (< or =54 fM) 2-3 orders of magnitude lower than other SPR devices
199 was toxin serotype-specific and detected 55 fM BoNT/A (1 LD50/ml) in 5 min and 0.4 fM (0.01 LD50/ml)
201 ow limit of detection (LOD) (< 5 pg/mL or 56 fM), a wide dynamic range (> 6 orders of magnitude), hig
202 the limit of detection (LOD) at 1 pg/ml (57 fM) and a linear relationship between increasing TNF-alp
206 sensor demonstrates a detection limit of 6.6 fM and high selectivity when compared to other non-speci
207 f SOD1 for copper (dissociation constant = 6 fM) and the high intracellular concentrations of both SO
208 eelin displaces the high affinity (K(D) = 60 fM) binding of [(125)I]echistatin (a competitive integri
211 ng 365 nm excitation was determined to be 63 fM, which is 3 times lower than with the DELFIA solution
212 pM for both targets, with LOD values of 650 fM (160 amol) and 190 fM (50 amol) for the event-specifi
216 level by simple spectroscopic analysis (40.7 fM and 2.45fM as measured by UV-vis and dynamic light sc
223 lecules at concentrations c ranging from 750 fM (p > 90%) down to 75 aM (10(-18) molL(-1)) levels (p
224 amples whose concentrations ranged from 0.76 fM to 6.0 fM showed that cluster number has a linear rel
226 sperm can register a minimal gradient of 0.8 fM/microm and be attracted from as far away as 4.7 mm.
227 ions of 3'-MAP excited triplet states of 1.8 fM and above resulted in significant human rotavirus ina
230 kground noise at concentrations down to 58.8 fM with an interassay reproducibility (%RSD of n = 3) <
231 f detection = 1 pg/mL, corresponding to 58.8 fM) and EHI_182030 (10 pg/mL, corresponding 453 fM) with
235 s of endoglin at concentrations as low as 83 fM with high detection specificity and has a three-order
237 f n = 3) < 17.2%, and in buffer down to 5.88 fM with an interassay reproducibility (% RSD, n = 3) of
238 thod enables detection of miRNAs as low as 9 fM and allows the discrimination of one base mismatched
241 ion limit for direct-impact voltammetry (900 fM), and is more than 30 times smaller than the previous
242 re successful in detecting as little as 2.94 fM of pathogen DNA, and using crude extractions of a pat
244 Fluorescence bursts were measured from a fM solution of DNA fragments ranging in size from 7 to 1
245 -cone signals of opposite polarity (-sM and +fM) cancel at low frequencies, but then constructively i
246 input signals of the same polarity (+sM and +fM) sum at low frequencies, but then destructively inter
247 spite their high avidity (K(d) approximately fM, lifetime approximately 4 days), immunity protein rel
248 inhibition of HGF/SF-induced uPA activity by fM-GAi is not uncommon, in that several human tumor glio
251 imes basal uPA activity; and (3) not only do fM-GAi derivatives strongly inhibit uPA activity but the
253 its of 100 attomolar (aM) and 10 femtomolar (fM) in pure samples for two ELISA assays with low and hi
254 vity for uranyl with a Kd of 7.4 femtomolar (fM) and >10,000-fold selectivity over other metal ions.
256 nanofiber-based system realizes femtomolar (fM) sensitivity toward complementary target DNA, and dem
257 lar calcium release channel (RyR) with high (fM) potency and provides a functional link between DDH a
258 romatic, and the fast, non-opponent inputs (+fM and +fL) as achromatic, both contribute to flicker ph
259 s in culture demonstrated that extremely low fM-nM concentrations of morphine and many other bimodall
261 cy, inducing full channel openings at lower (fM) toxin concentrations whereas at higher pM concentrat
262 4 mug (time-dependent) of modern carbon (MC, fM approximately 1) and 4.1 +/- 5.5 mug (time-independen
263 d for the non-opponent luminance mechanism (+fM and +fL) may still generate spectrally opponent signa
264 at successfully detected BPA at femto molar (fM) levels, which is an improvement over prior work by a
268 nt with pM naloxone or naltrexone (NTX) plus fM-nM morphine blocked the excitatory effects and unmask
270 annel has fast M- and L-cone input signals (+fM and +fL), and slow, spectrally opponent cone input si
272 theory, the assay could be sensitive to sub-fM analyte because beads attached via single-immune comp
276 en Ags are present at concentrations down to fM levels, specifically bound Abs can be scored by count
278 n leiomyosarcoma cells are also sensitive to fM-GAi; (2) fM-GAi drugs only display inhibitory activit
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