1 Glutathione S-transferase pulldown and
far Western analyses demonstrate that the interaction al
2 As assessed by
far Western analyses TraM interacts with TraR by directl
3 Both
far-western analyses and immuno-pull-down assays indicat
4 Using protein-protein pull-down and
far-Western analyses, we further show that the presence
5 ng to cellular proteins based on large-scale
far-western analyses.
6 tion of apoJ/XIP8 with Ku70 was confirmed by
far-western analyses.
7 tly with MLH1 in the yeast-two hybrid assay;
far Western analysis and co-immunoprecipitations confirm
8 measured in the absence of poly(A) RNA using
far Western analysis and confirmed by direct fluorescenc
9 We have used
Far Western analysis and phosphopeptide competition assa
10 Far Western analysis and the rebinding interaction with
11 s sequence (MRPFL) was shown to bind PCNA by
far Western analysis and to compete with p50 for binding
12 Far Western analysis demonstrated a direct association b
13 A trimeric
far Western analysis demonstrated the formation of a ter
14 Far Western analysis of N- and C-terminal deletion mutan
15 Far Western analysis revealed that PELP1 interacts with
16 Far Western analysis revealed that ZO-2 can directly bin
17 Far Western analysis reveals that SHP-2, via its Src hom
18 Far Western analysis shows that eIF3-p44 interacts stron
19 In
Far Western analysis, 29 distinct proteins were identifi
20 Coimmunoprecipitation,
far Western analysis, and glutathione S-transferase bind
21 a human placenta cDNA expression library by
Far Western analysis.
22 lated p62(DOK) was bound by SHP-1 C453S in a
far Western analysis.
23 osphorylated by RSK and used as ligands in a
far Western analysis; only those containing Ser(P)(703)
24 d in vitro to isolated denatured keratins in
Far-Western analysis and to native IFs in pull-down assa
25 umulation along the nuclear periphery and in
far-Western analysis bound to proteins which comigrated
26 nteracted with apobec-1 was also detected by
far-Western analysis in the eluate from the wild-type RN
27 Far-Western analysis suggests increased phosphorylation
28 In the current work, we performed
Far-Western analysis to identify PfPP2C substrates.
29 Using the yeast two-hybrid system and
far-Western analysis, we verified the importance of this
30 gion in protein interactions was verified by
Far-Western analysis.
31 PCNA-binding motif was shown to bind PCNA by
far-Western analysis.
32 Furthermore, "
far Western"
analysis suggested that the largest subunit
33 In
far western and northern North America, the host reservo
34 MG-I, as modified by casein kinase II, using
far Western and protein-protein interaction solution ass
35 nd does so via a direct interaction based on
Far Western and reverse Far Western blotting.
36 We confirmed LOX-PL interactions using
far Western and solid phase binding assays.
37 1 and AP-1 was demonstrated both in vitro by
Far-Western and antibody pulldown assays with recombinan
38 Far-Western and MS analyses identified both well-establi
39 SF/SF2 using a yeast two-hybrid system and a
far Western assay.
40 phosphorylated PDGFR from control cells in a
Far Western assay.
41 Glutathione S-transferase pull-down and
far Western assays showed that ADP-ribosylated Crk-I or
42 ides in both immunoprecipitation and reverse
Far Western assays.
43 Far-Western assays demonstrated that PKBalpha-mediated S
44 Co-immunoprecipitation,
Far-Western assays, and two-hybrid assays showed that TI
45 0-kDa protein homologous with SRC-1/TIF2, by
far-Western-
based expression screening.
46 the GST pulldown, co-immunoprecipitation and
far-western binding assays.
47 tein experiments, coimmunoprecipitations and
Far Western blot analyses to demonstrate direct binding
48 Far Western blot analysis also indicated that the SH3 do
49 Far Western blot analysis confirmed the specific binding
50 Far Western blot analysis confirms that SAPK/JNK binds d
51 Coimmunoprecipitation assays and
far Western blot analysis demonstrate that this mutation
52 xperiments, subsequent studies using reverse
Far Western blot analysis demonstrated that only the car
53 quiescent cells is probably not direct since
Far Western blot analysis did not reveal the binding of
54 Moreover,
Far Western blot analysis identified at least three AKAP
55 Far Western blot analysis implicates A190 of Pol I as we
56 Far Western blot analysis suggested that the tandem SH2
57 Far Western blot analysis was used to demonstrate the bi
58 Far Western blot analysis with either Nck, the SH2 domai
59 eningitidis strains was detected by FACS and
Far Western blot analysis, and occurred in the absence o
60 This binding was confirmed by
Far Western blot analysis, sedimentation on a glycerol g
61 type 3 (CR3, CD11b/CD18) was used to probe a
Far Western blot of a detergent extract of Hc cell wall
62 recognized by DC, VLA-5 was used to probe a
Far Western blot of a yeast freeze/thaw extract (F/TE) t
63 interact tightly and specifically, both on a
far Western blot of yeast vacuolar proteins and in the y
64 wild-type but not kinase-inactive JAK2 in a
far Western blot.
65 Real-time polymerase chain reaction, (
far) Western blot, immunoprecipitation, and immunocytoch
66 the domain of VP2 required for binding VP1,
far-Western blot analyses using a series of truncated VP
67 Here we used
far-Western blot analysis and microscale thermophoresis
68 Far-Western blot analysis established that HMW1B interac
69 to bind only to the beta-subunit of eIF2 by
far-Western blot analysis.
70 Structure-function analyses using
far-Western blot and transient cotransfection assays rev
71 on of PCSK9 activity, and we demonstrated by
far-Western blot assay that the M1 and M2 domains are ne
72 A was detected with the yeast two-hybrid and
far-Western blot assays.
73 C10258 (MFn8, Lsa66), and LIC10537 (MFn9) by
far-Western blot assays.
74 o I in enzyme-linked immunosorbent assay and
far-Western blot assays.
75 the pea aphid, Acyrthosiphon pisum, using a
far-Western blot method.
76 xible competitive binding assay based on the
far-Western blot technique, in which a battery of Src ho
77 ith Ace from OG1RF mutanolysin extracts on a
far-Western blot.
78 A protein (
far Western)
blot analysis revealed that dTAFIII105, pre
79 ombinant Fg chains and truncation mutants in
Far Western blots and solid-phase binding assays.
80 size from 120 to 200 kDa were identified on
Far Western blots for their ability to bind pTP.
81 Comparative
far Western blots have shown that hTAF(II)135 interacts
82 Using the peptides as probes in
far Western blots showed direct binding of the phosphory
83 ation experiments with knockdown of GAB2 and
Far-Western blots proved the direct interaction of SHP2
84 In "
far Western"
blots of mouse embryonic protein extracts,
85 Binding assays and
far Western blotting analysis demonstrated association o
86 Binding assays and
far Western blotting analysis, using glutathione S-trans
87 Far Western blotting and two-hybrid analyses detect a di
88 Far Western blotting revealed the presence of an approxi
89 ELISA and
Far Western blotting showed that recombinant vimentin bo
90 Far Western blotting showed that this interaction was li
91 Moreover,
far Western blotting using platelet membrane proteins re
92 Far Western blotting with a CRKL-SH3 glutathione S-trans
93 acts directly with DNA polymerase beta using
far Western blotting, affinity precipitation and yeast t
94 PAK2 with CIN85 SH3 domains was confirmed by
Far Western blotting.
95 interaction based on Far Western and reverse
Far Western blotting.
96 Using
far-Western blotting analysis of chemically cleaved and
97 Furthermore,
far-Western blotting analysis shows that SopE directly i
98 n HPV type 11 (HPV-11) E1-binding protein by
far-Western blotting and by microsequence analyses of a
99 The
far-Western blotting and co-immunoprecipitation assays d
100 In
far-Western blotting and guanine nucleotide saturation s
101 Initially, using a combination of
far-western blotting and phosphoCTD affinity chromatogra
102 By
Far-Western blotting and yeast two-hybrid assay, we demo
103 Far-Western blotting detected interaction of SATB1 and C
104 Far-Western blotting identified proteins of 150 and 240
105 sociates to amplify its functional capacity,
far-Western blotting of cholangiocyte epithelial cell pr
106 Far-Western blotting of EBP50 isolated by two-dimensiona
107 Interacting protein partners indicated by
far-Western blotting were confirmed by immunoprecipitati
108 By
far-Western blotting, and verified by coimmunoprecipitat
109 in-binding assay (VOPBA) in combination with
far-Western blotting, gradient-purified EAV particles we
110 phosphorylation by quantitative Western and
far-Western blotting, mass spectrometry, and computation
111 Using copurification analysis and
far-Western blotting, we found that Asp2 and Asp3 could
112 t binding to the NM could be demonstrated by
far-Western blotting.
113 ne, immunoprecipitation, immunoblotting, and
far-Western blotting.
114 action of VirB9 and VirB6-2 was confirmed by
far-Western blotting.
115 and LAT upon CRP stimulation of platelets by
far-Western blotting.
116 nd that comigrated with that detected by pTP
far-Western blotting.
117 system was in contrast to a strong signal by
far-Western blotting.
118 ): 1) phospho-specific mass spectrometry; 2)
far-Western blotting; and 3) live cell single-molecule i
119 Using interaction "
Far Western"
blotting assays, we systematically tested f
120 teract with Sp1 in vitro as analyzed by West(
far)-Western,
electrophoretic mobility shift assay-super
121 hat-GTPgammaSN terminus was analyzed using a
far Western method for examining Galphat-GTPgammaS prote
122 Using
Far Western overlay assay, we have identified additional
123 Far-Western overlays of soluble extracts of cauliflower
124 C terminus (C1aB) was first identified via a
far-Western peptide screen and used to create a prothera
125 We have used
Far Western screening and in vitro interaction assays to
126 Far Western/
solid phase assays established TINag binding
127 shown by mammalian two-hybrid, pulldown, and
far Western studies.
128 munoprecipitation, quantitative imaging, and
far-Western studies with cells expressing wild type, as
129 polyclonal or C3b monoclonal antibodies in a
far-Western technique followed by mass spectroscopy to i
130 Using a
Far Western type of analysis, we detected several potent
131 Far Westerns with digoxigenin-conjugated protein phospha
132 her co-immunoprecipitation experiments or in
Far Westerns with the SH2 domains of these two proteins.
133 e demonstrated by surface plasmon resonance,
far-western,
yeast two-hybrid, recombinant- and native-c