ライフサイエンスコーパス: farnesol

1 become diploid could derive from its use of farnesol.

2 o farnesal, which is subsequently reduced to farnesol.

3 ed by altering both endogenous and exogenous farnesol.

4 e which received a control injection without farnesol.

5 % starting from commercially available (E,E)-farnesol.

6 s inhibited by adding either alpha factor or farnesol.

7 nation of palmitate and of the sesquiterpene farnesol.

8 uired ergosterol for growth and produced E,E-farnesol.

9 did aggravate the fungal apoptotic effect of farnesol.

10 gs treated with zaragozic acid A or rats fed farnesol.

11 but was able to target to the cytoplasm with farnesol.

12 catalyze the interconversion of farnesal and farnesol.

13 ed in the presence of geranylgeranol but not farnesol.

14 d by the C. albicans quorum-sensing molecule farnesol.

15 induction of inflammatory gene expression by farnesol.

16 ase which converts farnesyl pyrophosphate to farnesol.

17 esol with formation of 9-hydroxyfarnesol and farnesol 10,11- and 2,3-epoxides.

18 route to the three geometric isomers of E,E-farnesol (12, 13, and 14) has been developed.

19 Mice receiving C. albicans intravenously and farnesol (20 mM) orally had enhanced mortality (P < 0.03

20 tion to the acyclic allylic alcohols (2Z,6E)-farnesol (6.7%) and nerolidol (3.6%), five cyclic sesqui

21 cAMP-Efg1 signalling cascade is inhibited by farnesol, a C. albicans autoregulatory factor, and small

22 ctivity of 3OC12HSL is compared with that of farnesol, a C. albicans-produced molecule also with a C1

23 e restores LTP to wild-type levels; however, farnesol, a chemically related compound, does not substi

24 que modulation of N-type calcium channels by farnesol, a dephosphorylated intermediate of the mammali

25 th animal and human arteries have shown that farnesol, a natural 15-carbon (C15) isoprenoid, is an in

26 ted reductase degradation by the addition of farnesol, a natural product of mevalonate metabolism, to

27 his inoculation releases the inhibition from farnesol, a quorum-sensing molecule of C. albicans, that

28 At micromolar concentrations, farnesol acts as a relatively non-discriminatory rapid o

29 the appearance of protein degradation after farnesol addition in vitro.

30 Within 15 min of farnesol addition, decreased transcript levels of pqsA,

31 In all experiments, mice administered farnesol alone or Tween 80 alone remained normal through

32 nthesized from newly and previously prepared farnesol analogs.

33 l regulation of vascular tone by farnesol or farnesol analogues.

34 Finally, the effect of farnesol and AFC on the NE response was reproduced in hu

35 olidol were more effective than trans, trans-farnesol and alpha-humulene.

36 dentification of GbTPS1 and GbTPS2, encoding farnesol and bisabolene synthases, respectively.

37 By feeding [1-14C]farnesol and comparing the mass of the dicarboxylic acid

38 eceptor that mediates attraction behavior to farnesol and demonstrates an effective approach to deorp

39 eral cAMP-controlled outputs are affected by farnesol and dodecanol, our findings suggest that these

40 Farnesol and farnesyl phosphate kinases have also been r

41 s, including several exotic alcohols such as farnesol and geraniol.

42 New fluorescent analogues of farnesol and geranylgeraniol have been prepared and then

43 CoAR activity, or the prenylation precursors farnesol and geranylgeraniol.

44 Effects of farnesol and JH on INV and transglutaminase mRNA levels

45 Both farnesol and JH stimulated INV and transglutaminase prom

46 The isoprenoids farnesol and juvenile hormone III (JH), metabolites of t

47 The FXR activators, all-trans farnesol and juvenile hormone III, also accelerated epid

48 yl pyrophosphate (FPP)-derived side products farnesol and nerolidol.

49 lative Th1-inclination property of menthone, farnesol and oridonin may be applied to improve Th2-skew

50 secretion ratios, suggesting that menthone, farnesol and oridonin may have a relative Th1-inclinatio

51 Menthone, farnesol and oridonin treatments did not markedly increa

52 substrate oxidation toward the center of the farnesol and palmitate molecules.

53 the substrate, and in the case of C(73-84), farnesol and palmitate oxidation was inhibited by 1 and

54 and GC-MS were used to identify products of farnesol and palmitate oxidation.

55 Farnesol and squalene are without effect.

56 ndida albicans secretes micromolar levels of farnesol and that accumulation of farnesol in vitro prev

57 attraction behavior to low concentrations of farnesol and that Or83c receptor mutants are defective f

58 ls and raise the possibility that endogenous farnesol and the mevalonate pathway are implicated in ne

59 This mutant (KWN2) produced six times less farnesol and was ca. 4.2 times less pathogenic than its

60 eed1Delta/Delta also excretes 10X more farnesol and while able to form hyphae, it cannot mainta

61 mpounds include certain sterols, oxysterols, farnesol, and geranylgeraniol, as well as the diphosphat

62 c terpenoid polyalkenes, including geraniol, farnesol, and geranylgeraniol, providing an efficient an

63 d -deficient terpenes derived from geraniol, farnesol, and nerol, thereby enabling the effective synt

64 y were partially reversed in the presence of farnesol, and treatment of mesothelioma cells with a spe

65 Because germ-tube formation is inhibited by farnesol, another quorum-sensing molecule, this process

66 Moreover, the effects of farnesol appear to be mediated by the FadA heterotrimeri

67 further evidence that the in vivo effects of farnesol are physiologically meaningful and specific for

68 eine, 1,4-diphenyl-2-butene, and trans,trans-farnesol are shown to inhibit competitively human, horse

69 We identified farnesol as a potent and specific activator for the orph

70 Candida albicans excretes E,E-farnesol as a virulence factor and quorum sensing molecu

71 ssion in recombinant yeast cells established farnesol as the preferred substrate of the FOLK-encoded

72 t (1) that leads to selective epoxidation of farnesol at the 6,7-position, remote from the hydroxyl d

73 ation of MrBI-1 reduced fungal resistance to farnesol but not to hydrogen peroxide, suggesting that M

74 ays, we now show that the pathway derivative farnesol causes Hmg2p to undergo a change to a less fold

75 Our findings demonstrate that in vitro, farnesol causes reductase to become detergent insoluble

76 n Candida albicans, exposure to the oxylipin farnesol causes the regulation of specific genes involve

77 chemical structures were tested: trans,trans-farnesol, cis-nerolidol, (-)-alpha-bisabolol, trans-beta

78 of the sesquiterpenic compounds trans, trans-farnesol, cis-nerolidol, alpha-humulene and guaiazulene,

79 Thus, reduced endogenous farnesol decreased virulence, while providing exogenous

80 tification and characterization of an insect farnesol dehydrogenase (AaSDR-1) that oxidizes farnesol

81 Farnesol dehydrogenase activity was not detected in the

82 , Arabidopsis membranes are shown to possess farnesol dehydrogenase activity.

83 n, growth and development were impaired in a farnesol-dependent manner when A. nidulans was co-cultiv

84 esol, then to farnesoic acid, and finally to farnesol-derived dicarboxylic acids (FDDCAs) which would

85 ently synthesized by anionic coupling of two farnesol-derived diepoxides, which have arisen from elec

86 tablishing that these dicarboxylic acids are farnesol-derived.

87 We finally demonstrated that farnesol did not affect Ca2+-sensitive pathways implicat

88 What does farnesol do in vivo?

89 e of this study was to determine the role of farnesol during infection with a well-established mouse

90 ectedly, at the levels detected (25-50 muM), farnesol enhanced S. mutans-biofilm cell growth, microco

91 To determine whether farnesol enhances the virulence of C. albicans by modula

92 BChls c were found to be farnesol esterified and geranylgeraniol esterified.

93 In contrast, mice pretreated with farnesol exhibited an unexpected elevation in IL-5 level

94 In species of Aspergillus, farnesol exposure induces apoptosis-like changes and alt

95 f Nicotiana tabacum cell cultures to utilize farnesol (F-OH) for sterol and sesquiterpene biosynthesi

96 Apigenin (Api) and tt-farnesol (Far) are two naturally occurring agents that a

97 With regard to exogenous farnesol, farnesol was administered either intraperitone

98 Farnesol (FOH) and other isoprenoid alcohols induce apop

99 of lovastatin with geranylgeraniol (GGOH) or farnesol (FOH) to investigate the role of protein prenyl

100 Interestingly, addition of farnesol (FOL) with lovastatin, to stimulate protein far

101 mone, which could potentially also transport farnesol for virulence and quorum sensing.

102 alysis, thus suggesting a possible action of farnesol from within the intracellular space.

103 was reversed by geranylgeraniol, but not by farnesol, further confirming that inhibition of geranylg

104 titate incorporation of exogenously provided farnesol, geranylgeraniol, and unnatural analogs of thes

105 farnesol synthesis, and relationships among farnesol, germ tube formation and hyphal maintenance.

106 To determine whether farnesol has a direct effect on macrophage production of

107 An alpha-phosphono lactone derivative of farnesol has been prepared, in both racemic and nonracem

108 We show that externally added farnesol has no effect on hyphal morphogenesis; instead,

109 nsible for the sequential phosphorylation of farnesol have not been identified and the physiological

110 016) identified eed1Delta/Delta as the first farnesol hypersensitive mutant of C. albicans.

111 The eed1 farnesol hypersensitivity can be explained by higher int

112 mice (n = 40) injected with 1.0 ml of 20 mM farnesol i.p. had enhanced mortality (P < 0.03), and the

113 er capacity against DPPH, while trans, trans-farnesol (IC50=1.81mM) and cis-nerolidol (IC50=1.48mM) w

114 Therefore, the role of farnesol in systemic candidiasis is likely due to its ab

115 nal experiments demonstrated the presence of farnesol in the brain (rodents and humans) at physiologi

116 Background farnesol in the cell-free extract was also retained by t

117 In this study we examined the effect of farnesol in the filamentous fungus Aspergillus nidulans.

118 levels of farnesol and that accumulation of farnesol in vitro prevents the yeast-to-mycelium convers

119 Taken together, our data suggest that farnesol, in addition to its quorum-sensing function tha

120 Finally, topical applications of farnesol increased mRNA and protein levels of the differ

121 creased virulence, while providing exogenous farnesol increased virulence.

122 Farnesol increases resistance to oxidative stress in C.

123 reactive oxygen species (ROS) participate in farnesol-induced apoptosis.

124 Additional studies implicate PrpA in farnesol-induced cell death and in the initiation of ase

125 ght be part of a prosurvival response during farnesol-induced ER stress.

126 ckdown of MEK1/2 or MSK1 expression inhibits farnesol-induced expression of CXCL3, IL-1alpha, and COX

127 We provide evidence indicating that the farnesol-induced phosphorylation of p65/RelA at Ser(276)

128 However, at 250 nM, farnesol induces an N-type channel-specific hyperpolariz

129 of human lung adenocarcinoma H460 cells with farnesol induces the expression of a number of immune re

130 It was further demonstrated that AFC and farnesol inhibited KCl and NaF-induced contractions, sug

131 promoter fused to lacZ similarly showed that farnesol inhibited PQS-stimulated transcription.

132 Farnesol inhibited production of both IL-12 p40 and p70

133 The latter is triggered by the release from farnesol inhibition of Cup9 degradation and consequently

134 repression of SOK1 expression in response to farnesol inhibition.

135 Interestingly, farnesol inhibits Cup9 degradation mediated by the N-end

136 Here, we show that farnesol inhibits hyphal initiation mainly through block

137 rnesol dehydrogenase (AaSDR-1) that oxidizes farnesol into farnesal, a precursor of JH, in the CA.

138 g the highest affinity for the conversion of farnesol into farnesal.

139 Based on these results, we conclude that farnesol is a nonsterol, mevalonate-derived product that

140 Farnesol is a quorum-sensing molecule produced by C. alb

141 Altogether, our results indicate that farnesol is a selective, high affinity inhibitor of N-ty

142 Farnesol is a sesquiterpene produced by many organisms,

143 Altogether, our results indicate that farnesol is an inhibitor of vascular smooth muscle Ca2+

144 Farnesol is an intermediate in juvenile hormone biosynth

145 that the induction of inflammatory genes by farnesol is mediated by the activation of the NF-kappaB

146 The three farnesol isomers were converted to the corresponding iso

147 hese questions, we confirmed the presence of farnesol kinase activity in Arabidopsis (Arabidopsis tha

148 unction mutations in the FOLK gene abolished farnesol kinase activity, caused an abscisic acid-hypers

149 These observations demonstrate a role for farnesol kinase in negative regulation of abscisic acid

150 falciparum-infected erythrocytes with [(3)H]farnesol labels 50- and 22-28-kDa proteins, whereas [(3)

151 these enzymes do not explain differences in farnesol levels implicating involvement of additional fa

152 We further show that intracellular farnesol levels increase significantly after mevalonate

153 om these studies showed that apigenin and tt-farnesol may enhance the cariostatic effectiveness of fl

154 Taken together, these data suggest that farnesol may play a role in disease pathogenesis, either

155 on, and overexpression of SOK1 overcomes the farnesol-mediated inhibition of hyphal initiation.

156 rovide molecular evidence for a link between farnesol metabolism, abiotic stress signaling and flower

157 These results suggest that oxidation of farnesol might be a rate-limiting step in JH III synthes

158 The activation of the NF-kappaB pathway by farnesol might be part of a prosurvival response during

159 NAD(+)-dependent dehydrogenase that oxidizes farnesol more efficiently than other prenyl alcohol subs

160 ersible, dose-dependent inhibitory effect of farnesol on L-type Ca2+ currents (IC50 = 2.2 microM).

161 vitro system was used to study the effect of farnesol on reductase degradation.

162 ns grown with intermediate concentrations of farnesol or dodecanol indicated a link between cells wit

163 In cultures containing farnesol or dodecanol, hypha formation was restored upon

164 e increased in abundance in cells grown with farnesol or dodecanol.

165 ssary for the repression of hyphal growth by farnesol or dodecanol.

166 ducing stimuli, grew as yeast in medium with farnesol or dodecanol; the heat shock sensitivity of the

167 armacological regulation of vascular tone by farnesol or farnesol analogues.

168 uman keratinocytes (NHK) treated with either farnesol or JH, even at low calcium concentrations (0.03

169 oleic acid), farnesoid X-activated receptor (farnesol or juvenile hormone III), or liver X receptor (

170 plained by higher internal concentrations of farnesol or lower thresholds for response.

171 ontrast, isoprenoids, such as nerolidol, cis-farnesol, or geranylgeraniol, or metabolites in the chol

172 een identified and the physiological role of farnesol phosphorylation has not been fully elucidated.

173 bition of isoprenylation, geranylgeraniol or farnesol prevented accumulation in S phase.

174 and Th2 cytokines, mice were pretreated with farnesol prior to intravenous infection with a sublethal

175 peritoneal macrophages were pretreated with farnesol prior to stimulation with IFN-gamma plus lipopo

176 of farnesol, we propose that it responds to farnesol produced by other fungi.

177 P. aeruginosa, suggesting that the amount of farnesol produced by the fungus is sufficient to impact

178 travenous infection with a sublethal dose of farnesol-producing C. albicans.

179 DPP3 reconstituted (KWN4) regained both its farnesol production levels and pathogenicity.

180 acterized LysR binding site, suggesting that farnesol promoted a non-productive interaction between P

181 th 1,4-diphenyl-2-butene or with trans,trans-farnesol provide molecular insights into these specifici

182 ar pools of geranylgeranyl pyrophosphate and farnesol pyrophosphate and thereby inhibits both geranyl

183 enoid geranylgeraniol, but not the 15-carbon farnesol, raising the possibility that the nonsterol pot

184 stions, including the existence of potential farnesol receptors and transporters, regulation of farne

185 We report here that farnesol reduced KCl- and norepinephrine-dependent cytos

186 In these cells, farnesol reduced KCl-induced [Ca2+]i transients and mimi

187 that mevalonate and geranylgeraniol, but not farnesol, released the lovastatin G1 block.

188 he CD36 homolog Snmp1 is required for normal farnesol response kinetics.

189 aintenance of hyphal growth may increase the farnesol response threshold.

190 uncation and deletion experiments revealed a farnesol-responsive region (-2452 to -1880 base pairs (b

191 the AP-1 site at -2116 to -2110 bp abolished farnesol responsiveness, identical to effects by peroxis

192 atin-treated cells, geranylgeraniol, but not farnesol, restored replication complex assembly and vira

193 E,E-farnesol results in up to an 8.5-fold increase in macrop

194 , quercetin, trans-ferulic acid, trans,trans-farnesol, rutin, gallic acid or sinapic acid).

195 Therefore, modulation of farnesol secretion to stimulate host immune recognition

196 Farnesol signaling was characterized as a quorum-sensing

197 Pretreatment with farnesol significantly reduced the elevation of both IFN

198 to chocolate, but not lavender, butanol, or farnesol, so that an interaction of route and odorant ma

199 mobility shift assays showed that, like PQS, farnesol stimulated PqsR binding to the pqsA promoter at

200 Here, we show that farnesol stimulates robust activation of Or83c-expressin

201 nt by numerous criteria, highly specific for farnesol structure, and requires an intact Hmg2p sterol-

202 ol receptors and transporters, regulation of farnesol synthesis, and relationships among farnesol, ge

203 on-specific pyrophosphatases responsible for farnesol synthesis.

204 detected in kidneys from mice receiving i.p. farnesol than in those from mice receiving control i.p.

205 Two farnesyl analogues were used: farnesol, the natural dephosphorylated form of farnesyl

206 or farnesyl diphosphate is its conversion to farnesol, then to farnesoic acid, and finally to farneso

207 DPH, the purified CYP4C7 metabolized (2E,6E)-farnesol to a more polar product that was identified by

208 However, after the addition of farnesol to cells in vivo, calnexin remains stable, wher

209 Here, we report that the addition of farnesol to cultures of Pseudomonas aeruginosa, an oppor

210 d with mammalian enzymes, the ability to use farnesol to label both farnesyl and geranylgeranyl moiet

211 Finally, direct addition of trans,trans-farnesol to the culture medium caused the rapid inductio

212 erved in them by the addition of geraniol or farnesol to the media.

213 ed in explants incubated with clofibrate and farnesol together in suboptimal concentrations which alo

214 d receptor mRNA was not detected in NHK, but farnesol treatment increased activities of both a PPAR r

215 Farnesol treatment reduces the level of IkappaBalpha and

216 With regard to exogenous farnesol, farnesol was administered either intraperitoneally (i.p.

217 urthermore, the increase in PPRE activity by farnesol was dependent upon PPARalpha in CV-1 cells.

218 Farnesol was measured using gas chromatography-mass spec

219 The effect of i.p. farnesol was more pronounced (P < 0.04) when mice were i

220 frq(10) or wc-2Delta effect, i.e., geraniol/farnesol was not required for a visible rhythm.

221 of urinary dicarboxylic acids from rats fed farnesol was virtually identical to that produced by tre

222 For endogenous farnesol, we created a knockout mutation in DPP3, the ge

223 ulans does not secrete detectable amounts of farnesol, we propose that it responds to farnesol produc

224 e dimorphic fungus Candida albicans secretes farnesol, which acts as a quorum-sensing molecule and pr

225 Exogenous farnesol, which enhances membrane protein prenylation, r

226 The C. albicans quorum sensing molecule farnesol, which inhibits Cyr1 and represses filamentatio

227 formate and the presence of Candida-derived farnesol, which is commonly known to exhibit antibacteri

228 E,E-Farnesol, which is secreted by white cells only, is a po

229 Farnesol, which is toxic to plant cells at high concentr

230 osed models showing possible interactions of farnesol with a protected Thr side chain and backbone NH

231 cro Prep High Q and conversion of FPP to E,E-farnesol with alkaline phosphatase.

232 all chimeric proteins catalyzed oxidation of farnesol with formation of 9-hydroxyfarnesol and farneso

233 ncubation with geranylgeraniol, but not with farnesol, with concurrent reversal of the inhibition of