ライフサイエンスコーパス: feeder cell

1 d for expanding these cells in vitro without feeder cells.

2 ed and used to generate embryonic fibroblast feeder cells.

3 rum in the presence of mitomycin-treated 3T3 feeder cells.

4 lated NGFR-expressing cells were free of PA6 feeder cells.

5 le factor (or factors) released by apoptotic feeder cells.

6 ot lead to immortalization in the absence of feeder cells.

7 tin (perlecan, fibulin-2), in the absence of feeder cells.

8 ated with radiation-induced apoptosis of the feeder cells.

9 e Notch ligand Dll4 on hESC-derived vascular feeder cells.

10 B-CLL cells died after removal of macrophage feeder cells.

11 rent to human stem-cell xenoculture on mouse feeder cells.

12 nating potential variability caused by using feeder cells.

13 genitors through coculture of hESCs with OP9 feeder cells.

14 ith Jagged-1 knockdown mesothelial and tumor feeder cells.

15 occurred in both the absence and presence of feeder cells.

16 oribosyltransferase (Hprt) gene and grown on feeder cells.

17 culture conditions with embryonic fibroblast feeder cells.

18 onic bodies in the absence of murine stromal feeder cells.

19 liminates the requirement for animal-derived feeder cells.

20 reactive proliferative response to syngeneic feeder cells.

21 xtension to human iPS cells cultured without feeder cells.

22 d expanded by mitogen and IL-2 on allogeneic feeder cells.

23 the infected cells were grown on fibroblast feeder cells.

24 unction derived from autologous or xenogenic feeder cells.

25 ing in the absence of exogenous cytokines or feeder cells.

26 m in the presence of mitomycin C-treated 3T3 feeder cells.

27 In short-term cultures with IL-12 and feeder cells, a proportion of the immature cells acquire

28 varian cancer cells with Jagged-1-expressing feeder cells activated the promoter activity of candidat

29 solating gene-corrected stem cells free from feeder cells after 3-5 days culture without drugs.

30 After reaching 80% confluence, 3T3 feeder cells and any contaminating fibroblasts were remo

31 yos were transplanted onto neonatal cortical feeder cells and assessed for their ability to generate

32 defined components such as mouse-derived 3T3 feeder cells and fetal bovine serum.

33 Initial derivation is on feeder cells and is followed by adaptation to a feeder-f

34 monstrated that physical contact between the feeder cells and keratinocytes is not required for induc

35 Both feeder cells and Rho kinase inhibition are required for

36 The combination of irradiated fibroblast feeder cells and Rho kinase inhibitor, Y-27632, conditio

37 3T3-J2 feeder cells and ROCK inhibition allowed rapid expansion

38 unknown and variable factors associated with feeder cells and serum.

39 tory culture without the use of heterologous feeder cells and their viability was demonstrated in viv

40 nued cell proliferation is dependent on both feeder cells and Y-27632, and the conditionally reprogra

41 and anti-CD28 monoclonal antibodies, CD4(+) feeder cells, and interleukin 2, provided for marked exp

42 crodrops seeded with mitotically inactivated feeder cells, and then connected with neighboring microd

43 major advantages of senescent fibroblasts as feeder cells are (i) the need to establish primary cultu

44 cient long-term LCL outgrowth and fibroblast feeder cells are not required.

45 n in co-culture with post-mitotic fibroblast feeder cells as compared with keratinocytes grown on tis

46 gene-corrected cells were obtained free from feeder cells at a "purity" of >30%, enriched >2,000-fold

47 In MS-5 feeder cell-based long-term cultures that supported the

48 s that differentiate in this time or require feeder cells, because the feeders must be drug resistant

49 kines IL-2, IL-21 and irradiated 3T3-msCD40L feeder cells can successfully stimulate switch-memory B

50 cient source of autologous iPS cells and, as feeder cells, can also maintain iPS and ES cell pluripot

51 ematopoietic progenitors was determined in a feeder cell coculture system and assayed by quantitating

52 d by using various empirical combinations of feeder cells, conditioned media, cytokines, growth facto

53 Thus, child cells exposed to feeder cell culture represent a novel model system in wh

54 Using a novel feeder cell-culture system, we observed long-term (>pass

55 nic stem cells (ES cells) in vitro depend on feeder cell-derived growth factors that are largely unid

56 Compared with ESCs grown on feeder cells, ESCs grown in feeder cell-free conditions

57 hat keratinocytes cultured in the absence of feeder cells exhibit a migratory phenotype and suggest t

58 mal differentiation was triggered on stromal feeder cells followed by regional specification by means

59 tocol also describes how ASCs can be used as feeder cells for maintenance of other pluripotent stem c

60 th ESCs grown on feeder cells, ESCs grown in feeder cell-free conditions exhibited decreased immunosu

61 em cell-based, chemically-defined, serum and feeder cell-free culture system, we show that the AhR is

62 y human B cell progenitors, we established a feeder cell-free in vitro system allowing the developmen

63 Here we report establishment of an in vitro feeder-cell-free LSC expansion and three-dimensional cor

64 ith other methods, our protocol does not use feeder cells, has a minimum dependence on proteins (purm

65 inocytes grown in co-culture with fibroblast feeder cells have an extended in vitro lifespan and dela

66 ale to stem cells that can be cultured (with feeder cells, if necessary) for >/=3 days.

67 ltures and irradiated cells commonly used as feeder cells in cloning techniques.

68 urified progenitors were plated onto stromal feeder cells in the presence of a large excess of differ

69 nitial TCR stimulation, but neither the PBMC feeder cells in the REP or the activated TIL expressed 4

70 m cells, we investigated whether Y-27632 and feeder cells induced a stem-like phenotype.

71 or (Y-27632), in combination with fibroblast feeder cells, induces normal and tumor epithelial cells

72 ures from experimental animals for preparing feeder cells is obviated; (ii) the risk of contamination

73 d, more importantly, that irradiation of the feeder cells is required for this induction.

74 of embryoid body, sera from animals, and the feeder cells isolated from mouse.

75 idomas can be immortal, they may depend on a feeder cell layer and may be genetically unstable.

76 ce of interleukin-2 (IL-2) and an allogeneic feeder cell layer, or IL-2 and other hematopoietic growt

77 ansion in vitro in the absence of serum or a feeder cell layer, suggesting that additional signals ar

78 itor cell-purification steps or support by a feeder cell layer.

79 n the absence of exogenous growth factors or feeder cell layers.

80 d) transgenic fish using a zebrafish ovarian feeder cell line (OFC3) that was engineered to express z

81 latency by long-term cultivation on the H80 feeder cell line in the absence of TCR stimulation.

82 of the cell culture system was the use of a feeder cell line that was initiated from ovaries of a tr

83 The feeder cell line was selected in G418 and engineered to

84 ivated mouse embryonic fibroblasts (MEFs) or feeder cells of human origin.

85 ined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and

86 ES) cells rely on growth factors provided by feeder cells or exogenously to maintain their pluripoten

87 Moreover, the use of human feeder cells reduces the risk of zoonosis.

88 Removal of feeder cells resulted in rapid differentiation of DCs an

89 us telomerase expression and co-culture with feeder cells results in efficient extension of lifespan

90 ulated into spinner flasks containing HEL299 feeder cells seated on microcarrier beads.

91 hematopoietic precursors in vitro use either feeder cell, serum, conditioned culture medium or embryo

92 mically defined conditions in the absence of feeder cells, serum, and leukemia inhibitory factor.

93 e MEF and human placental stromal fibroblast feeder cells, some proteins were only expressed in suppo

94 activity alone when grown in co-culture with feeder cells, suggesting that loss of the p16(INK4a)/Rb

95 growth in culture, whether in the fibroblast feeder cell system or in the specialized K-sfm medium fo

96 induce pluripotency, and requires the use of feeder cells that add complexity and variability to the

97 ated every 2 weeks with irradiated alogeneic feeder cells, that had similar functional properties thu

98 ep for 1 month were transferred onto Sertoli feeder cells, they differentiated into functional sperm

99 ly immunodeficient children were cultured on feeder cells, they well supported R5, but not X4 HIV-1 r

100 ouse and human T cells on antigen-expressing feeder cells to develop higher-affinity TCRs.

101 verrode the requirement of serum factors and feeder cells to maintain mESCs in culture.

102 are then dispersed and plated on irradiated feeder cells to propagate and isolate individual iPSC cl

103 m cell typically rely on protein matrices or feeder cells to support attachment and growth, while mec

104 helial cells cultured in the presence of 3T3 feeder cells undergo biochemical differentiation, as evi

105 d colonies separated from one another by the feeder cells were detached as a sheet from the dish and

106 ree culture system, devoid of animal-derived feeder cells, were sorted by relative cell size and char

107 actors, we have established sub-lines of STO feeder cells which exhibit variable ability in supportin

108 ves coculture of irradiated mouse fibroblast feeder cells with normal and tumor human epithelial cell