ライフサイエンスコーパス: filamentous phage

1 a hybrid between adeno-associated virus and filamentous phage.

2 re expressed only when cells are infected by filamentous phage.

3 ed in the context of the pIII protein of M13 filamentous phage.

4 ngle-chain Fv fragments (scFvs) displayed on filamentous phage.

5 e the genome of any previously characterized filamentous phage.

6 biopanning with a random peptide library on filamentous phage.

7 natorial library displayed on the surface of filamentous phage.

8 in vivo whether it supports the assembly of filamentous phages.

9 protein (pII) of Escherichia coli F-specific filamentous phages.

10 nnel required for the assembly and export of filamentous phage across the outer membrane of Escherich

11 Filamentous phage also exploits TolAIII to enter target

12 Genomic analysis suggests that additional filamentous phages also exploit chromosome-encoded outer

13 library by display of expressed proteins on filamentous phage and affinity selection for binding to

14 The repertoires of domains were displayed on filamentous phage and denatured (at 80 degrees C in pH 7

15 Fab fragment of the antibody 17E8 (h17E8) on filamentous phage and sorted for binding to an immobiliz

16 x 10(6) different members) was displayed on filamentous phage and subjected to affinity selection wi

17 gene expression of the minor coat protein of filamentous phage and suggest that some ORF- clones may

18 This report summarizes the properties of filamentous phage and the proteins required for their as

19 Biogenesis of both filamentous phage and type-IV pili involves the assembly

20 ith similarities to open reading frames from filamentous phages and cryptic phage elements.

21 be successfully displayed on the surface of filamentous phage, and after phosphorylation in vitro, c

22 rt of group A colicins, for infection by the filamentous phage, and for maintenance of the integrity

23 sing cassette mutagenesis, then displayed on filamentous phage, and screened for proteins retaining h

24 ger zinc finger proteins (ZFPs) displayed on filamentous phage, and selected for ZFPs that bound alon

25 Filamentous phage are elongated semiflexible ssDNA virus

26 Although all filamentous phages are constructed of chiral components,

27 The filamentous phages are important as cloning vectors, veh

28 Filamentous phages are now the most widely used vehicles

29 Filamentous phage assemble at the membrane of infected c

30 how here that ATP hydrolysis is required for filamentous phage assembly and that the proton motive fo

31 bacteria, type II and type III secretion and filamentous phage assembly systems use related outer mem

32 e functional and structural requirements for filamentous phage assembly, and they may provide guideli

33 CTXphi is unusual among filamentous phages because it can either replicate as a

34 CTXphi is unusual among filamentous phages because it encodes a repressor and fo

35 aquaticus DNA polymerase I is displayed on a filamentous phage by fusing it to a pIII coat protein, a

36 These data demonstrate that N. gonorrhoeae filamentous phage can induce antibodies with anti-gonoco

37 provide information on the requirements for filamentous phage coat assembly, and provide improved sc

38 uirements for protein incorporation into the filamentous phage coat.

39 Filamentous phages consist of a single-stranded DNA geno

40 wn as Shiga toxin producing E. coli) and the filamentous phage CTXphi, that carries genes encoding ch

41 rains of Vibrio cholerae are lysogens of the filamentous phage CTXphi, which carries the genes for ch

42 d converts to a pathogen upon infection by a filamentous phage, CTXPhi, that transmits the cholera to

43 CT), which is encoded within the genome of a filamentous phage, CTXphi.

44 stroms, which is large enough to accommodate filamentous phage (diameter of 65 angstroms).

45 atelet lysis and oxidation in vitro, using a filamentous phage display 7-mer peptide library.

46 Filamentous phage display has been extensively used to s

47 icity of OmpT by two complementary substrate filamentous phage display methods: (i) in situ cleavage

48 We used filamentous phage display to isolate a panel of peptides

49 Binding parameters of 26 different filamentous phages, displaying peptides selective for en

50 e DNA sequences have been determined possess filamentous phage DNA sequences.

51 PulD was 32% identical with a portion of the filamentous phage-encoded protein, pIV.

52 This suggests that the pathway for filamentous phage entry into cells is conserved between

53 Finally, PH75 is the only filamentous phage exhibiting UVRR markers diagnostic of

54 The fact that both colicin N and filamentous phage exploit TolAIII in a similar manner, w

55 In vivo selection of filamentous phage expressing B. burgdorferi protein frag

56 any bacteria to secrete proteins, toxins, or filamentous phages; extrude type IV pili (T4P); or take

57 Filamentous phage f1 encodes protein IV (pIV), a protein

58 Filamentous phage f1 exits its Escherichia coli host wit

59 me overlapping genes II and X carried by the filamentous phage f1 genome are proteins with required b

60 Filamentous phage f1 is exported from its Escherichia co

61 Infection of Escherichia coli by the filamentous phage f1 is initiated by binding of the phag

62 of in-frame overlapping genes II and X from filamentous phage f1 to determine if translational contr

63 The secretin encoded by filamentous phage f1 was purified.

64 , F-actin, microtubules, and viruses such as filamentous phage fd and tobacco mosaic virus (TMV), as

65 Infection of Escherichia coli by the filamentous phage fd starts with the binding of the N2 d

66 the g3p-D1D2 fragment (residues 2-217) from filamentous phage fd to 1.9 A resolution and compared it

67 A filamentous phage, fd, also failed to produce plaques on

68 hain and aromatic side chains in the class I filamentous phage, fd.

69 pparent in capsid subunits of the mesophilic filamentous phages, fd, Pf1, and Pf3, previously examine

70 oter was fused to the protein coding part of filamentous phage gene III (gIII).

71 torial libraries displayed on the surface of filamentous phage has become an important methodology fo

72 Display of peptide libraries on filamentous phage has led to the identification of pepti

73 hough many different display platforms using filamentous phage have been described, no comprehensive

74 hat encodes TCP has also been described as a filamentous phage; however, these sequences are unlike t

75 d within the genome of CTXphi, an integrated filamentous phage identified in 1996.

76 The genes are those in the filamentous phage IKe genome, which encode the abundant

77 Effective use of a filamentous phage in such applications requires an under

78 sequences of the integration sites of other filamentous phages indicates that the XerCD recombinases

79 hich can be induced in Escherichia coli upon filamentous phage infection (specifically phage secretin

80 ty of stressful conditions or agents such as filamentous phage infection, ethanol treatment, osmotic

81 ide new evidence for a conserved pathway for filamentous phage infection.

82 Because the Raman spectrum of a filamentous phage is strongly dependent on the relative

83 guish isotropic and anisotropic solutions of filamentous phages is consistent with previously reporte

84 Unlike other filamentous phage libraries, fd-tet-based libraries are

85 A filamentous phage library expressing random peptides was

86 We have used a filamentous phage library that displays random linear he

87 nly used bicelle, poly(ethylene glycol), and filamentous phage liquid crystalline media, dipolar coup

88 eriplasm of E. coli or at the surface of the filamentous phage M13 retained the immunological specifi

89 eophylline-binding RNA aptamer, dissolved in filamentous phage medium, and used to investigate the lo

90 enesis, protein secretion, DNA transfer, and filamentous phage morphogenesis systems are thought to p

91 n selection to conserve the basic pattern of filamentous phage mRNAs, the major mRNAs representing ge

92 cteristic of cholera, are encoded on a novel filamentous phage named CTXPhi, has resulted in a renewe

93 been developed that is capable of assembling filamentous phage only upon addition of exogenous thiore

94 -enrichment experiments (biopanning) using a filamentous phage peptide library.

95 comparative (1)H NMR metabonomic analyses of filamentous phage pf1 infection in planktonic cultures o

96 ame symmetry (class II) as in the mesophilic filamentous phages Pf1 and Pf3.

97 It is strongly induced by filamentous phage pIV and its bacterial homologues, and

98 Furthermore, filamentous phage promoted bacterial adhesion to mucin a

99 Filamentous phage protein III (pIII), located at one end

100 Assembly and export of filamentous phage requires four non-capsid proteins: the

101 Addition of this domain to the filamentous phage secretin, pIV, or to the unrelated mal

102 bulk production, bio-nanostructures such as filamentous phage showed great potential in materials sy

103 ogenic state and a new tool for the study of filamentous phage structure.

104 The ability of filamentous phages, such as 493, to transfer large amoun

105 ology were preserved and re-formatted in the filamentous phage system to allow direct selection of ca

106 lexible tether to the pIII coat protein of a filamentous phage that also displays the catalyst.

107 CTXphi is a filamentous phage that encodes cholera toxin, one of the

108 XPhi is a Vibrio cholerae-specific temperate filamentous phage that encodes cholera toxin.

109 t regulate production of CTXphi, a temperate filamentous phage that infects Vibrio cholerae and encod

110 ctxB) are encoded in the genome of CTXphi, a filamentous phage that infects Vibrio cholerae.

111 st within the same filaments, in contrast to filamentous phages that have been described as belonging

112 Filamentous phages that infect Escherichia coli require

113 f CTXphi in a way similar to those for other filamentous phages, the CTXphi RS2-encoded gene products

114 using alignment media of stretched gels and filamentous phage to determine the relative orientation

115 his article, we have analyzed the ability of filamentous phage to display a stable form of green fluo

116 he binding of the HBsAg-specific peptides on filamentous phage to mAbs.

117 l OM integrity, and for class A colicins and filamentous phages to enter cells, its precise role has

118 Thus, CTXphi, like the E. coli filamentous phages, uses both a pilus and TolQRA to ente

119 on cell viability, Fab yield and display on filamentous phage using different vectors and bacterial

120 In a filamentous phage vector containing BR96 V region sequen

121 ich are often poorly displayed with standard filamentous phage vectors.

122 Filamentous phage was the first display platform employe

123 ng peptides displayed on the pIII protein of filamentous phage was used in biopanning experiments aga

124 Interaction of fd or M13 filamentous phage with a chloroform/water interface indu