ライフサイエンスコーパス: filensin

1 d two beaded filament proteins (phakinin and filensin).

2 fiber cell: CP49 (phakinin) and CP115/CP95 (filensin).

3 in beta-B1-crystallin, Hsp-90, spectrin and filensin.

4 olytic N-acetylation and N-myristoylation of filensin.

5 sion of large quantities of MIP26, CP49, and filensin.

6 eta- and gamma-crystallins, MIP26, CP49, and filensin.

7 alysis of both CP49 and its assembly partner filensin.

8 ulted in the coprecipitation of phakinin and filensin.

9 Immunoelectron microscopy confirmed that filensin and AQP0 were present in the same membrane comp

10 d sites of phosphorylation and truncation in filensin and CP49 and revealed two unusual PTMs: postpro

11 The lens beaded filament proteins filensin and CP49 are phosphorylated proteins that under

12 copy, regions of colocalization of AQP0 with filensin and CP49 at the fiber cell plasma membrane in t

13 separated by SDS-PAGE, and the corresponding filensin and CP49 bands were digested by trypsin, Lys C,

14 Filensin and CP49 were enriched by urea extraction of le

15 filament network consisting of the proteins filensin and CP49, and it was recently reported that the

16 Colocalization of AQP0 with filensin and CP49, two proteins identified after mass sp

17 lens-specific intermediate filament proteins filensin and CP49.

18 ajor intrinsic protein 26 (MIP26), CP49, and filensin and morphologic changes such as cell multilayer

19 Nine phosphorylation sites on filensin and seven phosphorylation sites on CP49 were id

20 The mechanism by which the absence of filensin and the beaded filament affects optical clarity

21 used to define the distribution of phakosin, filensin, and beaded filaments.

22 sis was used to assess mRNA levels for CP49, filensin, and gammaS-crystallin (control).

23 ce were probed for the IF proteins phakosin, filensin, and vimentin, using light microscope immunocyt

24 Phakosin and filensin are lens fiber cell-specific intermediate filam

25 ediate filament proteins phakinin (CP49) and filensin (CP115), and vimentin during a mild 20-minute h

26 Messenger RNA levels for filensin, CP49's assembly partner, were normal, but prot

27 haA-, alphaB-, beta-, and gamma-crystallins, filensin, CP49, and MIP/aquaporin 0.

28 acid occurs at the major truncation sites of filensin (D431) and of CP49 (D37).

29 Filensin expression was increased further when cells wer

30 zation including ankyrin-B, spectrin, NrCAM, filensin, ezrin and desmoyokin.

31 Animals homozygous for the mutated filensin gene and wild-type CP49 gene were compared with

32 Disruption of the filensin gene successfully blocked production of filensi

33 1 and the transcriptional start site of the filensin gene.

34 cell-specific intermediate filament protein filensin is essential for beaded filament assembly.

35 In phakosin and filensin knockout mice, initial lens development and the

36 ccurred in previously generated phakosin and filensin knockout mice.

37 Filensin knockouts began to show evidence of light-scatt

38 Filensin levels were sharply reduced, although filensin

39 lensin levels were sharply reduced, although filensin mRNA levels appeared unchanged.

40 nsin gene successfully blocked production of filensin mRNA, reduced levels of filensin's assembly par

41 at FVB/N mice do not have detectable CP49 or filensin protein in the lens, whereas C57BL/6 mice have

42 (residues 33-44), betaA4 (residues 106-117), filensin (residues 78-90), and phakinin (residues 77-89)

43 Low-level affinity was seen between the filensin rod domain and periplakin.

44 oduction of filensin mRNA, reduced levels of filensin's assembly partner CP49, and prevented the asse

45 e the protein levels of its assembly partner filensin, suggesting a mechanism for the regulation of b

46 termediate phenotype, showing a reduction in filensin transcript and moderate light-scattering at 5 m

47 It includes phakosin and filensin, two divergent members of the intermediate fila

48 Filensin was found to be truncated at D431 and L39, and

49 Phakosin and filensin were first detected in the very latest stages o

50 ed, and putative database sequence errors of filensin were identified.

51 ed expression of the beaded filament protein filensin when compared with control cells.